Professional Documents
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Review
Introduction of Microbial Biopolymers in Soil
Treatment for Future Environmentally-Friendly
and Sustainable Geotechnical Engineering
Ilhan Chang 1,† , Jooyoung Im 2,† and Gye-Chun Cho 2, *,†
1 Geotechnical Engineering Research Institute, Korea Institute of Civil Engineering and Building
Technology (KICT), Goyang 10223, Korea; ilhanchang@kict.re.kr
2 Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and
Technology (KAIST), Daejeon 34141, Korea; jooyoungim@kaist.ac.kr
* Correspondence: gyechun@kaist.edu; Tel.: +82-42-350-3622; Fax: +82-42-350-7210
† These authors contributed equally to this work.
Abstract: Soil treatment and improvement is commonly performed in the field of geotechnical
engineering. Methods and materials to achieve this such as soil stabilization and mixing with
cementitious binders have been utilized in engineered soil applications since the beginning of
human civilization. Demand for environment-friendly and sustainable alternatives is currently rising.
Since cement, the most commonly applied and effective soil treatment material, is responsible for
heavy greenhouse gas emissions, alternatives such as geosynthetics, chemical polymers, geopolymers,
microbial induction, and biopolymers are being actively studied. This study provides an overall
review of the recent applications of biopolymers in geotechnical engineering. Biopolymers are
microbially induced polymers that are high-tensile, innocuous, and eco-friendly. Soil–biopolymer
interactions and related soil strengthening mechanisms are discussed in the context of recent
experimental and microscopic studies. In addition, the economic feasibility of biopolymer
implementation in the field is analyzed in comparison to ordinary cement, from environmental
perspectives. Findings from this study demonstrate that biopolymers have strong potential to
replace cement as a soil treatment material within the context of environment-friendly construction
and development. Moreover, continuing research is suggested to ensure performance in terms of
practical implementation, reliability, and durability of in situ biopolymer applications for geotechnical
engineering purposes.
1. Introduction
Geotechnical engineering, especially the treatment and usage of soil (or earth) in construction,
is a long-standing technical field, dating to the beginning of human civilization. In ancient
Mesopotamia and Babylonia, mud was used as a common construction material for various forms
of bricks (i.e., sun-dried and kiln-fired) to create the architecture of the city. Sumerians widely used
bitumen as a binder to enhance the strength and durability of earth walls [1]. In ancient Egypt,
earth engineering methods were developed to create river embankments to control the annual flooding
of the Nile river [2]. Engineers in ancient China used a sticky rice mortar that contained amylopectin
as a construction binder for the Great Wall [3].
As civilization has advanced, the durability and strength of construction materials has improved
as well. The discovery of natural pozzolan materials such as volcanic ash improved construction
materials, including the well-known concrete of ancient Rome. The representative formula for Roman
concrete was a mixture of volcanic dust with a binder such as gypsum or lime, with added aggregates,
and it was used to create highly durable structures including arches and domes [4]. After the Industrial
Revolution, ordinary cement (i.e., Portland cement) became the most widely used construction and
building material, not only for structural purposes, but also for soil stabilization and strengthening
purposes (Figure 1).
The main purpose of soil treatment and improvement (i.e., engineered soil) is to enhance the
engineering characteristics of a particular soil, including its strength (i.e., resistance), hydraulic
conductivity, and durability against repeating wetting and drying, as well as for environmental
revitalization [5]. Two primary methods are conventionally applied to produce engineered soil:
mechanical improvement and chemical treatment. Mechanical improvement is a process of reinforcing
the strength of the soil through physical processes such as compaction, drainage, external loading
(e.g., surcharge), consolidation, or other means. Chemical treatment involves chemical reactions such
as hydration or pozzolanic reactions inside the soil to create artificial binding, such as the use of
calcium silicate hydrate (C-S-H) between soil particles [5].
As an alternative to such traditional soil treatment and improvement techniques, biological
approaches are now being actively investigated in the field of geotechnical engineering, including
microbe injection and byproduct precipitation. In particular, microbial induced polymers—or
biopolymers—have been introduced as a new type of construction binder, especially for soil treatment
and improvement.
To date, most studies on these applications of biopolymers have been experimental efforts that
have produced preliminary findings and analyses, and the number of theoretical explanations and
case studies of practical implementation in the literature are still limited. In response, this paper
provides a detailed review of biopolymer applications in geotechnical engineering including the most
recent studies. In this review, strengthening mechanisms between typical biopolymers and soils
based on microscopic inter-particle interactions are summarized. The advantages and disadvantages
of biopolymer applications are compared with those of existing soil engineering methods. Finally,
the potential for practical implementation is evaluated via an economic feasibility analysis, including
environment-friendly considerations.
Sustainability 2016, 8, 251 3 of 23
2.1. CO2 Emissions related to Cement Usage in Civil and Geotechnical Engineering
Ordinary Portland cement currently dominates the field of materials used for construction
and civil engineering purposes: 5245 million tons of hydraulic (i.e., ordinary Portland) cement
were produced worldwide in 2012 [6]. Portland cement has multiple engineering benefits, such as
high strength and durability, workability, and hydraulicity, as well as low cost (60~100 USD/ton,
in developed countries), and these factors have contributed to its widespread use in various
applications for ground improvement, concrete structures, and pavement in civil and construction
engineering [7].
In geotechnical engineering projects, cement has been used in numerous formats, including
deep cement mixing (DCM), grouting, soil nails, and soil stabilization, and is now the most favored
material for engineered soil. However, despite cement’s benefits and its numerous applications,
the overdependence on and overuse of cement has given rise to a number of environmental concerns.
In the production of cement, the calcination of calcium carbonate (i.e., 5CaCO3 + 2SiO2 Ñ
3CaO¨ SiO2 + 2CaO¨ SiO2 + 5CO2 ) liberates 0.55 tons of CO2 per 1 ton of Portland cement, which is
then emitted to the atmosphere. In addition, approximately 0.4 tons of CO2 are emitted due to the
burning of carbon fuel during the production of 1 ton of cement, since calcination requires heat as high
as 1450˝ C. The total amount of CO2 emitted per each ton of cement produced is 0.95 tons [8].
In 1995, for example, 1453 M¨ tons of cement were produced worldwide, accounting for
approximately 5% of the global annual CO2 emissions [9]. By 2003, the amount of CO2 emissions
from cement production had increased to 7% of the global CO2 emissions [10]. This means that the
rate of CO2 emissions related to cement production (Figure 2a) has doubled during the past 30 years,
from 4.2% in 1980 to 9.0% in 2012. Moreover, the annual growth rate of cement related CO2 emission
continues to increase annually—it reached 10% in 2010—in spite of global efforts to reduce CO2
emissions by limiting its annual growth rate to less than 3% (Figure 2b).
Figure 2. CO2 emissions of cement through the years (after [11] (a) and [12] (b)).
Sustainability 2016, 8, 251 4 of 23
CO2 emissions related to the usage of cement in geotechnical applications (e.g., mixing, grouting,
soil stabilization) amount to approximately 2% of the total CO2 emissions by cement (i.e., 3.05 B¨ tons
in 2011) [11,13]. Thus, replacing 10% of cement usage with low-carbon materials in geotechnical
engineering implementations leads to a reduction of 6.1 M¨ tons of CO2 , which is close to 10% of the
annual CO2 emissions from Austria (66.68 M¨ tons) in 2012 [14].
separation, filtering, reinforcement, and drainage purposes across a large range of applications [31–33].
While such materials can provide higher strength, specifically tensile strength, the strengthening is
almost completely dependent on the material itself and not the soil, making them undesirable for
various engineering practices.
In the late 20th century, geotechnical engineers began introducing chemically synthesized
polymers as soil conditioners to replace the use of conventional binders, such as lime and cement,
for geotechnical engineering and agricultural purposes. Polyacrylamide (PAM) [34] is a simple
linear- or cross-linked polymer synthesized by acrylamide (i.e., CH2 CHCONH2 ) subunits, which
are non-toxic and hydrophilic [35]. Anionic polyacrylamide (PAM) has negative charge density and
induces electrostatic bonding with clay particles [36,37], which results in reduced soil erosion and
runoff control [38]. PAM consequently has been used in various fields such as agriculture, construction,
and military applications (e.g., temporary helicopter landing fields) as a soil erosion reduction
agent [38,39]. Sodium silicate (Na2 (SiO2 )n O) and other chemicals, including acrylics, lignosulfonates,
and phenolasts, have been widely implemented in soil grouting practices, especially for sandy soils.
However, chemically synthesized polymers and solutions have raised concerns about toxicity and
water pollution problems [40], and this has restricted the usage of chemical solutions near water
conservation regions in particular.
Meanwhile, electroosmotic chemical treatment (ECT) has been attempted in several studies to
improve the strength of soft soils [41,42]. However, the ECT method requires massive injection of
chemical solutions to produce an increase in osmotic pressure, and has not yet been accepted for
practical implementation [43].
Geopolymers are polymers synthesized from either organic or inorganic, such as silicon-based,
origins [44,45]. The geopolymerization process involves initial mixing in an alkaline solution,
which dissolves silicon and aluminum ions (i.e., alkali-activation) from soil or industrial byproducts
such as fly ash or blast furnace slag. Hydroxyl ions (2OH´ ) remaining in the alkaline solution then react
to form one free water molecule (H2 O) and an oxide ion (O2´ ) [46]. The released oxygens then form
monomers with the dissolved silicon and aluminum ions, which undergo condensation. Geopolymers
require isolated heating (above 60 ˝ C) for proper geopolymerization (hardening) [47,48], which can
limit site application for geotechnical engineering purposes. Studies have shown that geopolymers
are highly susceptible to water and show significant degradation in strength when saturated in water,
especially in acid solutions, due to depolymerization of the aluminosilicate polymers and removal of
silicic acid [49].
calcium ions [55]. The calcium carbonate precipitates bind with the soil grains to increase the strength
and stiffness of the soil [56].
Several studies have been performed to increase the strength and stiffness of soils using urease
positive microorganisms [53,57]. In these studies, the microorganism Sporosarcina pasteurii was injected
directly into the soil to allow for calcite precipitation by the microorganisms themselves.
The use of urea, CaCl2 , and urease leads to the precipitation of calcite through enzymatic processes
without microbial activities, which is suitable for soil strengthening and increasing stiffness [58]. MICP
has also been found to be applicable in various other fields, including the remediation of heavy
metals [59], CO2 sequestration [60], and the repair of concrete [61,62].
However, MICP has several shortcomings in terms of field implementation. MICP is most
applicable to coarse sediments, and accompanies difficulties when used with fine grained soils due to
infiltration problems: the pore sizes of fine grained sediments provide an unsuitable environment for
bacteria growth [63]. Moreover, the transport, cultivation, and fixation performance of bacteria is not
consistent. Thus, recent studies have adopted the use of urease enzyme instead of using bacteria to
promote the hydrolysis of urea, for the chemical precipitation of calcite [64]. Furthermore, the use of
MICP results in a highly concentrated ammonium chloride byproduct, which needs to be removed
and treated, generally in the form of soil flushing [65].
Even though the limitations of MICP are problematic, the bio-soil method has been shown to have
several promising features, including high strengthening, low environmental impact, self-proliferation,
and biodegradation. A number of researchers have thus focused on the direct use of biogenic excrement
(i.e., biopolymers) instead of attempting to cultivate the microorganisms in the soil.
The characteristics of common biopolymers are summarized in Table 1, and the following sections
review notable case studies in geotechnical engineering.
3.3.2. Chitosan
Chitosan (P-(1,4)-2-amino-2-deoxy-D-glucose) is the degraded form of chitin, found in the
exoskeletons of crustaceans and the cell walls of fungi. It is typically extracted by alkali sodium
hydroxide treatment. This hydrophilic biopolymer has been used in a variety of applications.
As a biorenewable, biocompatible, biodegradable, and biofunctional biopolymer, chitosan has been
used and researched in the fields of food/nutrition, material science, medical science, microbiology,
and immunology, as well as other fields such as agriculture, wastewater treatment, and degradation
products [81,90,91]. In geotechnical and geoenvironmental contexts, chitosan has been shown to be
capable of decontaminating groundwater containing contaminants such as copper (II) (Cu2+ ) and
phosphorus (P-) [92,93]. Chitosan coated sand particles have practical implications in filters for
Sustainability 2016, 8, 251 8 of 23
remediation of contaminated groundwater [81] and can create a suitable plugging effect by reducing
the hydraulic conductivity of sandy soils [94].
3.3.3. Curdlan
Curdlan is a high molecular weight linear β-1,3-glucan biopolymer produced by pathogenic
bacteria (e.g., Agrobacterium biobar, Alcaligenes faecalis) that forms an elastic gel when heated in an
aqueous solution [79]. Curdlan has been used as an additive in concrete mixtures as a superplasticizer
to improve workability and prevent cement-aggregate separation [95]. From a geotechnical perspective,
curdlan has been suggested for use in grouting due to its large scale soil clogging potential [96].
3.3.4. Beta-Glucan
Beta-glucans are biopolymers that consist of D-glucose monomers that are linked by glycosidic
bonds [97]. β-glucan is naturally found in various formations, such as in cellulose, bran, and the cell
walls of yeast, fungi, and bacteria [77].
A small amount (0.25%) of beta-glucan biopolymer (i.e., mass ratio to soil) in soil provides a
compressive strength value of 2650 kPa, comparable to that of a 10% cement mix (2170 kPa), while
0.5% beta-glucan mixing yields soil strength up to 4310 kPa [98]. From a geotechnical perspective,
the presence of beta-glucan in soil increases the Atterberg limit values as well as shear stiffness (G),
while it seems to have minor or even no effects on the constrained modulus (M) of soil [99]. A recent
study showed that beta-glucan treatment promotes vegetation growth in treated soils as well as high
strength, and in particular it promoted vegetation growth on barren soil, indicating its potential for
use as a countermeasure for desertification [100].
a similar gelation rheology and strengthening effect to agar gum, gellan gum is preferred for future
mass commercialization because it can be easily produced via microbial fermentation, whereas agar
gum has to be extracted from seaweed (i.e., algae).
(a)
(b)
(c)
(d)
Figure 4. Cont.
Figure 4. Cont.
Sustainability 2016, 8, 251 11 of 23
Figure 4. SEM images of biopolymer-soil mixtures: (a) Beta-glucan and Korea residual soil;
(b) Beta-glucan and glassbeads; (c) Xanthan gum and kaolin clay; (d) Xanthan gum and sand (Jumunjin,
Korea); (e) Gellan gum and kaolin clay; (f) Gellan gum and sand (Jumunjin, Korea); and (g) Agar gum
and kaolin clay (images from [98,103,106]).
However, this does not mean that biopolymers have a negligible impact in terms of their use with
sand particles, because well-graded soil with coarse particles treated with biopolymers shows higher
strength than that obtained with pure clay, such as kaolinite [103].
Figure 5 shows the inter-particle cohesion and friction angle variation of 1% gellan gum
biopolymer mixed soils, evaluated via direct shear tests. Although the unconfined compressive
strength (Figure 3) and cohesion of biopolymer mixed clay is much higher than that produced in
pure sand, the friction characteristics of sandy soils (i.e., sand-clay mixtures) are more appropriate
for practical applications than those of pure clay. Thus, the strengthening mechanism of biopolymers
Sustainability 2016, 8, 251 12 of 23
in ordinary soils (i.e., those containing both sand and clay) is believed to be a combination of the
formation of biopolymer-clayey soil matrices (i.e., cohesion enhancement) and friction improvement
via coarse particles acting as aggregates (Figure 6). Inter-particle cohesion enhancement depends
on the strength of the biopolymer-clayey soil matrices, which is higher with lower water content.
The friction angles of the soil increase with biopolymer treatment due to improved particle contact.
The stiffer dried gels add a substantial amount of strength with a small increase in the contact radius,
especially at lower water content, as opposed to the softer wet gels, which exhibit almost no increase
in strength with a small increase in the contact radius.
Figure 5. Direct shear test result of 1% gellan gum biopolymer mixed soils.
Figure 6. Schematic model for biopolymer treated soils: (a) chain type biopolymers with sandy soils;
(b) gel type biopolymers with sandy soils; (c) chain type biopolymer with clayey soils; and (d) gel type
biopolymers with clayey soils.
Sustainability 2016, 8, 251 13 of 23
5. Discussion
Moreover, biopolymers can be introduced into the soil by various practical modes of application
including mixing, injection, spraying, and grouting, and they can be used for building materials,
earth pavement, and farmland erosion prevention (Figure 7). Furthermore, biopolymers form a stable
gel matrix inside soil that does not damage the local ecosystem. Combined with their water retaining
properties in soil, biopolymers are thought to be capable of promoting vegetation growth.
The largest advantage of direct biopolymer implementation compared to other bio-soil methods
is that biopolymers can be produced ex-situ (i.e., exo-cultivation) and applied in-situ with a higher
degree of quality control, whereas MICP requires time-consuming in-situ cultivation. Moreover,
biopolymers can be commercially mass produced, and react with soil particles immediately after
mixing, which allows them to be utilized for temporary or rapid supporting purposes.
The material cost for 0.5% xanthan gum biopolymer for soil treatment has decreased from $250 to
$28 during the last three decades.
While the majority of biopolymers are currently quite expensive, the actual cost of their use
in geotechnical engineering largely depends on the choice of biopolymer. Additionally, the present
market prices of most candidate biopolymers are for food grade quality with very high purity, and that
standard results in significantly higher production cost. For geotechnical applications, edible grade
purity is unnecessary, and thus the price of biopolymers can be reasonably expected to be lower when
they are produced specifically for construction and geotechnical engineering applications.
xanthan gum (market price: www.alibaba.com), in 2014; c EU ETS (European Union Emission Trading Scheme)
carbon emission trade: 22 USD/ton of CO2 , in 2012.
With global greenhouse gas reduction efforts and carbon emission trading (considering a rate of
22 USD/ton CO2 ) [121], the CO2 emission trade imposed on soil-cement mixtures (with 10% cement)
has been estimated to be 2.75 USD, while 0.5% xanthan gum treatment would secure 0.55 USD CERs
(Certified Emission Reductions) for 1 ton of soil treatment, respectively. When this CO2 emission
trading is considered, in economic terms xanthan gum soil treatment becomes remarkably competitive,
being only 3.6% more expensive than cement, as seen in Table 3. Thus, especially when considering
the aim of environment-friendly construction and development, biopolymers have high potential to
replace high carbon emitting soil treatment materials.
Sustainability 2016, 8, 251 17 of 23
6. Conclusions
Ground enhancement methods have been intensively studied and developed over centuries.
The use of cement in geotechnical engineering could be considered the beginning of modern ground
enhancement methods, and numerous studies have since been performed to enhance the properties of
cement. However, in the late 20th century, environmental concerns gave rise to increasing demand for
Sustainability 2016, 8, 251 18 of 23
environment-friendly construction methods. The development of green cement and several possible
cement substitutes, including the direct use of biopolymers in the soil layer, have been proposed.
Recent studies have shown that biopolymers can strengthen soils, and they offer several
advantages in such applications, including being environment-friendly and effective at low
concentrations. Several studies have shown that the strengthening induced by biopolymer treatment
is maximized in the presence of fines, especially clay particles. For polysaccharide-type biopolymers,
hydroxyl groups on the polymer surfaces induce hydrogen bonding with water molecules, making
them hydrophilic, and enabling the formation of firm (i.e., viscous) hydrocolloids or hydrogels.
Meanwhile, when water is scarce, as in conditions of drought or dry soils, biopolymers can form
direct hydrogen bonds with clay particles, or indirect ionic bonds with these particles, in the presence
of intermediate ions such as alkali- or alkali earth- metal ions in the soil. Direct and indirect bonding
leads to the formation of a firm biopolymer-clay matrix, which provides a significant increase in soil
cohesion. Proper mixing of coarse particles, clay particles, and biopolymers is thus expected to provide
optimal strengthening effects, due to the combination of increased mechanical friction between coarse
particles, and a cementation effect between biopolymer-clay matrices.
In contrast to cement, biopolymers have strong potential to reduce carbon dioxide emissions.
Moreover, some biopolymers show functionality to support vegetation growth and stabilization,
which can be applied as countermeasures for farmland preservation and anti-desertification and
against other threats to environmental conservation.
Although their benefits are numerous, several challenges related to the use of biopolymers remain
to be addressed, including sensitivity to water, market costs, and possible biological degradation.
Overall, given the wide variety of available biopolymers, the flexibility of their modification,
and the numerous advantageous properties that they possess, the use of biopolymers in geotechnical
engineering appears to have a promising future.
Acknowledgments: The research described in this paper was financially supported by a National Research
Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2015R1A2A2A03006268), by a
grant from the Strategic Research Project (Development of Key Excavation Solutions for Expandable Urban
Underground Space) funded by the Korea Institute of Civil Engineering and Building Technology (KICT), and by
the KAIST End-Run Program (No. N01150661) supported by the Korea Ministry of Science, ICT and Future
Planning (MISP). The second author is supported by the “U-City Master and Doctor Course Grant (Education)
Program” under the Korea Ministry of Land, Infrastructure and Transport (MOLIT).
Author Contributions: Ilhan Chang and Gye-Chun Cho conceived and constructed the outline and context
of the review. Ilhan Chang and Jooyoung Im provided detailed reviews on previous studies and analyzed
the data. All authors wrote the paper together with different contributions (Jooyoung Im was responsible for
Sections 2–4; Ilhan Chang contributed to Sections 5 and 6; and Gye-Chun Cho provided an overall summary in
Sections 1 and 6).
Conflicts of Interest: The authors declare no conflict of interest.
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