Professional Documents
Culture Documents
1. Gloves
2. Tourniquet
3. Alcohol Prep Pads
4. Gauze pads or cotton
5. Needles and lancets
6. Evacuated tube holder or adapter
7. Needle disposal (sharps) container
8. Adhesive tapes
9. Permanent marker or pen
10. Appropriate tubes or sample container
Additional Information:
ANGLE of the needle – 15-30 ⁰
Tourniquet must be 3-4 inches above from the puncture site and do not leave tourniquet for
more than 1 minute.
Disinfecting the site by solutions i.e., 70%alcohol, should be done in concentric circle manner.
Order of Draw
Blood collection tubes must be drawn in a specific order to avoid cross-contamination of additives between
tubes. The recommended order of draw for evacuated tubes:
Invertions
MODE OF
ADDITIVE USES
ACTION
Coagulation tests
Sodium citrate Forms Calcium
(protime and
Light Blue salts to remove 3-4
prothrombin time), full
calcium
draw required
Serum separator
tube (SST)
None contains a gel at
Chemistries, Immunology
Yellow the bottom to 6-8
and Serology
separate blood
from serum on
centrifugation
Sodium Antiglycolytic
Glucoses, requires full draw
fluoride and agent preserves
Gray (may cause hemolysis if 6-8
potassium glucose up to 5
short draw)
oxalate days
Note: Tubes with additives must be thoroughly mixed. Erroneous test results may be obtained when the blood
is not thoroughly mixed with the additive.
LABELING THE SAMPLE
A properly labeled sample is essential so that the results of the test match the patient.
The key elements in labeling are:
NOTE: Both of the above MUST match the same on the requisition form.
Date, time and initials of the phlebotomist must be on the label of EACH tube.
Automated systems may include labels with bar codes along with Patient's ID
number.
3rd and 4th finder of non-dominant hand Heel punctures are best for newborn and infants
COMPLICATIONS
1. Fainting/Syncope
2. Hematoma
3. Thrombosis
4. Petichaie
5. Excessive bleeding
6. Siezures
7. Collapsed veins
8. Hemoconcentration
9. Hemolysis
10. Allergies