Professional Documents
Culture Documents
USER’S MANUAL
● Product guarantee
● Index
Foreword
At first, we really appreciate your purchase of TEK-II MINI 3-diff hematology analyzer.
Please read this user’s manual carefully before using, and conduct the operation correctly.
Please keep it properly after reading for future scan.
In order to ensure the safe operation of instruments, please abide by the following notices.
● This illumination content include all special fittings setting (sell separately).If you have not
purchased this kind of special fittings setting, please jump over this contents when reading this
operation manual.
● This instrument is only used for whole blood cell inspection analyzing, it may not work
normally except for this purpose.
● Please read illumination before using this instrument, and use it under knowing well this
illumination situation.
● Please operate this instrument under the management of engineers who have well trained by
training company pointed by Tecom.
● Please check whether this instrument can work normally by testing QC material when using
this instrument, incorrect testing result may cause wrong diagnosis.
● Do not disassemble or reassemble the unit of instrument without the permission of our
company because it is possible to cause the danger and the damage of instrument.
● Don’t apply the method not indicated in user’s manual because it is possible to cause the
damage to instrument.
● Exporting the test result is recommended. That is for prevent the storage history data lost
because of the instrument failure.
● The approved technicians by Tecom conduct assembling, augmenting, reassembling,
improving and repairing the equipments. Otherwise, Tecom will not responsible for it.
I. I
Copyright and declaration
Tecom Science Corporation has the copyright of this unpublicized manual and has the right to treat
it as conventional data. This manual is only used as operate and maintain TECOM products for
your reference. Others have no right to make it public.
This manual contains some proper data protected by the copyright law. It can not be duplicated, or
translated to other languages without written consent from Tecom Science Corporation.
TECOM does not make any guarantee to this material, including guarantee responsibility of
implied merchantability proposed to it for some specific purpose. Tecom is not responsible for the
mistakes in the material and the accidental of indirect loss caused by the actual use of this manual.
The display figure in this book may be a little difference with the actual display figure.
Due to the renewal of products, sometimes there would be some situations which disagree with the
content of this manual, please pardon us for not giving notice separately.
I. II
Product Guarantee
Guarantee period:
One year after the installation date.
Guarantee content:
Tecom will supply free amendment to customers due to the trouble caused by fault of our design,
manufacturing during the guarantee period, but Tecom will take relative action as amend method
according to the trouble content.
I. III
Using environment for instrument:
The relative service department of our company carries on the installation when purchase.
Instrument should meet the following conditions and be used under the corresponding
environment.
1. Less dust, good ventilation.
2. Avoid direct sunlight.
3. It is at least 20 cm far from the wall behind the instrument so as to radiate and connect
pipelines easily.
4. There must be a power supply or socket within 1.5 meters away from the machine.
5. Good horizontal level, the intensity and areas are enough for enduring and placing 4 sets
instrument (about 50Kg).All the reagent should be placed on the same horizontal level as the
instrument, shall not be higher or lower than the instrument, the waste barrel shall be placed on
the ground.
6. The room temperature should kept between 10 ~35 ,and the change of room temperature
shall within ±2 during testing process.
7. The room moisture shall not higher than 90%, no dew.
8. It is prohibited to use the instrument in the environment where the moisture is above 90%.If the
instrument is used in the environment where the temperature is below 10 or above 30 , it is
necessary to install an air conditioner.
9. No appreciable shake.
10. No acute change for power supply. (within AC220V±10%)
11. No near strong electro-magnetic disturbance (centrifuge, discharge device etc.)
12. There is sole grounding extremity.(the grounding resistance shall below 10Ω)
13. This instrument is affected by electromagnetic wave, and may affect the data, cause the
mistaking operation.
14. The instrument should stored within -10 55 , the relative humidity shall not higher than
95%, atmospheric pressure between 500hpa to 1060hpa, no corrosive gas, shall place it in good
ventilation and clean room.
I. IV
Directions for safe use
Before using, please read “Directions for safe Use” and using illumination carefully and conduct
the operation correctly.
For the sake of using instrument safely and correctly, and keep you and others or the possession far
away from the damage, we use various symbols and signs.
The meanings of symbols and signs are as follows:
Please understand its meanings fully, then read the body of this book.
I. V
Signs and meanings
О
Alternating current shut down(electrical source
cut)
grounding
Attention
To explain the important information in the
operating process and some special operating
skills. If not comply with the illumination, maybe
affect the output result or damage the products.
Warning In the lab, when contacting with any
Admonish users pay attention to the potential possible biohazardous materials,
dangers. There would do harm to human beings You should wear the standard
or damage the products if not complying with protective clothes and gloves and
them. comply with the prescriptions of
Warning safe operation or deal with them
Waste liquid have potential bio-infective hazard. according to the demand of local
government.
Warning
Some substances (samples, substance of quality
control, standard substance, waste liquor)have
potential bio-infective hazard.
Warning Contact the manufactory to recycle
Admonish users pay attention to the potential or deal with them according to the
dangers, electronic rubbish, easy to pollute demand of local government.
environment.
CE is the sign EU protect in accord, product
should comply with the requirement of Directive
98/79/EC.
Measurement sign
I. VI
Danger of electric shock
Remind the user to avoid getting electric shock.
Warning
I. VII
● In case the unusual odor appears, smoking is sent our, please cut off the power
supply at once, and pull out the plug from the outlet.
If continue to use it, there will be danger of fire accident, getting electric shock or getting
hurt.
Please contact us or our agent as soon as possible.
● The operator shouldn’t touch the inner circuit of the instrument, especially when the
hand is wet, which will result in the damage of instrument, or getting the electric
shock.
● Use the appointed tools or parts and components. The use and shift substitute is very
dangerous.
● Do not spill blood or reagent in the instrument, and do not insert pin and other
Otherwise there will be dangerous of short circuit and electric shock. In case the unusual
situation appears, Please cut off the power supply at once, and pull out the plug from the outlet.
Warning
I. VIII
Direction for electric source wire, voltage, connection and grounding:
● It is absolutely necessary to insert the electric source plug into the appointed outlet.
● When installing the instrument, the electric source or case must be grounded; otherwise it
will result in the unstableness of the instrument performances and the possibility of getting
● Don’t put the heavy thing on the electrical source wire or pull it tightly, otherwise it will
damage the electrical source wire so as to cause short circuit or will break the wire so as to
● Before opening the instrument to maintain its inner parts the electrical source must firstly be
cut off to prevent from getting the electrical shock or causing troubles.
cut off electrical source firstly, otherwise, there will be the danger of electrical shock or
Warning
I. IX
Biohazardous Material:
● Do not touch these materials (samples, substance of quality control, standard substance,
disposing waste liquor without careful, firstly wash by the disinfect liquor, then wash
evenly by soap.
● If contaminated by the blood or waste liquor, immediately wash with sterilized liquor and
then rinse with clean water, and follow the doctor’s instructions.
● Take more care when dealing with samples, must put on rubber glove, having the
possibility infected by viruses. In case touch the eyes or wound, firstly flush with plenty
Warning
Biohazardous Material:
● When disposing with the waste liquor, do not touch waste liquor directly, must put on
rubber glove, because there have the danger of potential biology epidemic. If touched by
waste liquor, flush with disinfect liquid firstly, and then wash with soap fully.
● Customers have the obligation to follow all the related regulations regarding the release of
Warning
I. X
Reagent using notice:
● If touched by eyes, must flush with plenty of water immediately, and follow the doctor’s
Instructions.
● If take by accident, call the doctor immediately and at the same time drink plenty of water
and sick.
● If the skin or hands are contacted with the reagent, please flush it with plenty of clean
water immediately.
Attention
I. XI
Direction for matched reagent:
● After sealing off, care that the dust, dirt or microbe and shouldn’t mix with in reagent.
● In the process of replacing and applying reagent, care that the bubble should not be
produced in it.
● When maintaining and repairing instruments, use appointed tools or the parts and
● Do not let anyone who doesn’t understand the instrument or hasn’t been trained specially
use the instrument at will. Anyone who uses the instrument must be trained specially, or
● If there is trouble with the instrument, please contact our company or our distributors. Our
company is responsible for repairing it. The guarantee period is shown in the contract.
● In case there is other problem with the instrument, please read the Chapter Eight of this
Operation Instruction Manual carefully. The person in charge of the instrument should
deal with the problem. If necessary, please contact our company or our distributors.
● Do not be installed where chemical medicines are stored or gas could be produced.
I. XIII
Chapter One Installation
2. Grounding
The power supply should adopt three-pin plug, If there is grounding jack in the outlet, the plug
may be inserted directly for using. If there is no grounding jack, the adapter must be used to
ground the earth wire.
3. Stable voltage
Power of 220V,50Hz is required, wave is required within ±10 .
Special power supply should be used for equipment, and should be used under stable voltage, if
it is conditional, high precision purified stable power supply should be equipment.
Warning
● It is absolutely necessary to insert the electric source plug into the appointed outlet.
● When installing the instrument, the electric source or case must be grounded; otherwise it
will result in the unstableness of the instrument performances and the possibility of getting
● Do not put the heavy thing on the electrical source wire or pull it tightly, otherwise it will
Damage the electrical source wire so as to cause short circuit or will break the wise so as to
1-1
Chapter One Installation
4. Connection of pipeline
Take out the pipeline of diluent, lyse, detergent and waste liquor. and connect it according to
the sign behind the equipment on one end, then connect the other end with the reagent (waste
liquor)barrel. Please pay attention to each sign of pipeline. Do not mix it and protect pipelines.
Do not fold it.
Paper outlet
Pd
Paperboard key
Printing Head
Boss
SEL Key Paper outlet
Front Plate LF Key Papering Axe
Transparent Indicator
Window Light Clip trough
Get the front plate down from the printer by moving the front plate forth with finger, please refer
to Fig.1
Fig.1
Get the bosses at two sides of the printing head with fingers and move them out, please refer to
Fig.2 and Fig.3
1-2
Chapter One Installation
Fig 2 Fig 3
Draw the printing head of papering place with hands, please refer to Fig.4.
Fig 4 Fig 5
Please make the paperboard key forth at the left side of printing head, please refer to Fig.5
The thermal paper has two sides, only the smooth side can be printed and the side must be adown;
Press the LF Key and the paper will put in the paper inlet slowly and please press the LF Key
Press the paperboard key down, please refer to Fig 6;Press the printing head into printer with
hand.
Refer to Fig.7.Put the papering axe into the paper hole. use thumb and forefinger to clamp both
Fig 6 Fig 7
1-3
Chapter One Installation
Fig 8 Fig 9
Fit the papering axe into the clip trough of the printer, please refer to Fig.8;push “SEL” Key and
the indicator light is on, make the paper out from the outlet with the front plate and cover the
front plate and then the printer keep in waiting condition, please refer to Fig.9
Note Thermal printer only uses special thermal paper and need not change the ribbon.
6. Connection of barcode scanner (expand choose the fittings)
Make sure the instrument is under the power off condition, it is better to pull out the power line
from the connection board, then connect the interface of code scanner with the interface of
scanner behind.
7. Connection outside printer (expand choose the fittings)
Make sure the instrument and printer are in the power off condition, it is better to pull out the
power line from the connection board, then connect the interface of printer to the USB
interface or PRINTER interface of the instrument behind.
8. Connection of computer (choose the fittings)
Make sure the instrument and computer are in the power off condition, it is better to pull out
the power line from the connection board, then connects the RS-232 interface of computer
mainboard with RS-232 interface of the equipment back through serial port wire. Please see the
Installation of online application software in reference part I.
Warning
● When connecting the peripheral fixings (printer and barcode scanner),it is necessary to cut
off electrical source firstly, otherwise, there will be the danger of electrical shock or cause
1-4
Chapter Two Introduction
Reagent of maintain:
Concentrate Cleaner
Reagent of QC substance:
QC substance.
Other consumables:
Printing paper
2-1
Chapter Two Introduction
2-2
Chapter Two Introduction
After adding the hemolytic reagent, the range of the white blood cell volume is 35—400fl
● Platelet Histogram
10 20 30 fl
2-3
Chapter Two Introduction
RBC/PLT 1 50000
or tip blood
Interface RS232
Volume 370mm×275mm×400mm
V. Performances of Instrument
PLT
Item Linearity Rel≥0.99
ative Coefficient r
WBC ≥0.99
RBC ≥0.99
HGB ≥0.99
2-4
Chapter Two Introduction
Record review
Soak and Rinse
Testing
Aperture
Prime Det
Prime Dil
Prime reagent
Menu Prime Lyse
Prime All
Calibration
Range
Setup
Preference
Time Setup
Target QC
QC
Float QC
Shut down
2-5
Chapter Two Introduction
Menu Record
F1 7 8 9 ESC
Thank you for use Testing
T E K -II Mini Analyzer F2 4 5 6 PgU
Automatic 3-diff Maintenan
Hematology Analyzer F3 1 2 3 PgD
QC
F4 0 PRI YES
Shut
F5 NO
xxxx-xx-xx xx-xx-xx
2.Key Explanation
Name Function
0~9 For the input of sample number, data of setting value, etc.
For the selection of menu, the cursor will be up, down, left and right while press
the keys
PgUp, PgDn For the change and read if there are several pages
Exit For the exit of the interface and back to the above interface
3. Explanation
2-6
Chapter Two Introduction
4. Testing Interface
Result
ID 001
Item Result Unit Item Result Unit
WBC 0.0 10^9/L LY% 0.0 %
RBC 0.00 10^12/L MID% 0.0 %
HGB 0 g/L GR% 0.0 %
HCT 0.000 L/L LY# 0.0 10^9/L
MCV 0.00 fL MID# 0.0 10^9/L
MCH 0.00 pg GR# 0.0 10^9/L
MCHC 0 g/L RDW-CV 0.0 %
PLT 0 10^9/L RDW-SD 0.0 fL
PDW 0.0 %
MPV 0.0 fL
PCT 0.000 L/L
Histogram ID. Set F2
ID. Set Press F2 and the cursor will stay in the“No:” inputting the new number can replace
[fl]
0 2 5 10 20 30
Next Page
2-7
Chapter Two Introduction
entirety, reagent used in this instrument is open, but in order to ensure good result, please ensure
that all the products were inspected by manufacturer. Propose do not use reagent or QC substance
supplied by different suppliers, otherwise the instrument can not obtain its performance target, The
reagents have not been inspected may supply irresponsible data. The reagent mentioned in this
text is refer to the special reagent used in TEK-II MINI automatic 3-diff hematology analyzer.
Each type of reagent shall be examined before using, damage of container may affect the
quality of reagent. If the package is damaged, please check if there is leakage or damped evidence.
If this situation exit, then the reagent can not be used. It is necessary to know its notice items
1. Reagent
The reagent matched to use in this instrument are: diluent, lyse, detergent.
● Diluent
Diluent will dilute the blood sample and at the same time it can supply with the similar
environment for blood plasma, to ensure the configuration of the blood cell within certain time,
● Lyse
Lyse can destroy red blood cell membrane quickly, and lessen the volume of cell
fragment to the volume that will be noninterference to the WBC counting, and meanwhile
change the configuration of WBC, divide into three diff to classified. The destroyed RBC
will release hemoglobin and reactive with lyse to come into being a special complex
● Detergent
Detergent is used to clean the pipeline and counting pool, this detergent is a specific
2-8
Chapter Two Introduction
● Concentrated detergent
Warning
● In case of entering into eyes by mistake, shall rinse with volumes of water promptly and
● In case of taking by mistake, shall call for doctor immediately and meanwhile drink
● In case of stain on hands or skin, shall rinse with volumes of water promptly.
Standard substance and QC substance are used to calibrate and quality control for the
instrument. Standard is a kind of whole blood product or latex atom for commercial producing, are
used for calibrate for instrument and ensure the veracity of testing result. The instrument has been
calibrated before leaving factory, but it may cause excursion during the process of transporting
and operating, system deviation can be found through QC, and it needs to calibrate for the
instrument.
QC substance can be commercial whole blood or fresh blood, is used for checkout whether
the instrument test is normal. Generally, QC substances have three types of high, mid, low value.
Running three types of QC substance can checkout the running state of instrument to ensure the
2-9
Chapter Two Introduction
Warning
contact with waste, immediately rinse with disinfect liquor at first and then wash with
soap completely.
● When polluted by blood, waste, please rinse with disinfect liquor, then flush with clean
● Pay more attention to that you shall wear rubber glove when deal with sample. It has
possibility of infected by pathogen. In case of it enter eyes or wound, shall rinse with
About550
Diluent 20L/bucket
samples/20L
Lyse About950
1L/bottle
samples/1L
Detergent 5/L bucket or About
1/L bottle 60-100mL/day
Note: The consumption of reagent is related to the quantity of everyday samples, times of start
2-10
Chapter Two Introduction
Attention
● After sealing off, care that the dust, dirt or microbe shouldn’t mix with reagent.
● In the process of replacing and applying reagent, care that the bubble should not be
produced in it.
● Use the reagent according to the notices recorded on the label or reagent.
● WBC ≤0.3×109/L
● HGB ≤1g/L
2-11
Chapter Three Principle
When the cell flows through the aperture rapidly, because the cell is not a good conductor, the
increase of voltage R of aperture is equivalent to the decrease of sectional area A of aperture under
the condition of a constant electric current, the instantaneous change of voltage appears and an
electric pulse is produced, the quantity of electric pulse is equal to the quantity of cells flowing
through the gem aperture. The magnitude of electric pulse is proportional to the magnitude of cells
so as to count the quantity of cells and determine the magnitude of cells.
1.
3-1
Chapter Three Principle
2. The MCH is automatically calculated using the instrument’s RBC and HGB measurement
3. The MCHC is automatically calculated using the HGB RBC and MCV. The computations
4. The distribution width of red blood cell: if the peak value of distribution curve of red blood
cell’s granularity is 100 , the distribution width of red blood cell is counted from the points
1.
3-2
Chapter Three Principle
L1 L2
5. The specific volume of platelet is evaluated by the following formula according to PLT and
MPV
6. The distribution width of platelet: if the peak value of distribution curve of platelet
granularity is 100 , the distribution width of red blood cell is counted from the points accounting
L1 L2
Explanation of Parameter
1. White Blood Cell (WBC)
White blood cell may be divided into three parts by mutual action of dilution and lyse:
lymphocytes group; mainly lymphocyte; mid-size cell group; mainly monocytes cell, eosinophils,
The test of white blood cell may be interfered by abnormal specimen, such as: the coacervation of
platelet, nucleated red cell, condensed globulin, abnormal lymphocyte etc., all these will cause
abnormal to the count of white blood cell and histogram. The drug having the cell’s toxicity and
1.
3-3
Chapter Three Principle
immunity inhibition used for chemotherapy may change the action of hemolytic agent on white
blood cell to cause the count of white blood cell decrease obviously.
2. Red Blood Cell (RBC)
The visible components of blood cell are contained in the dilution of red blood cell, including: red
blood cell, white blood cell and platelet. In the process of counting red blood cell the volume of
platelet is smaller than that of red blood cell, therefore the red blood cell may be divided from
platelet. The difference in normal proportion of white blood cell to red blood cell is very wide, so
the influence of white blood cell on red blood cell may neglect.
The test of red blood cell may be interfered by abnormal specimen, such as: cold agglutination
syndrome, the noticeable increase of white blood cell count, a large quantity of huge platelet etc.,
all these can cause abnormal to the count of red blood cell and histogram.
3. Hemoglobin (HGB)
After lyse is added to the diluted blood, the red blood cell is dissolved to release hemoglobin,
which is combined with the hemolytic agent to form the derivative of hemoglobin. The
The test of hemoglobin can be interfered by the abnormal specimen such as hyperlipemia, high
bilirubinemia disease etc., all these will cause abnormal to the test of hemoglobin.
4. Platelet (PLT)
The test of platelet can be interfered by the abnormal specimen, such as small red blood cell,
incomplete red blood cell, coacervation of platelet etc., all these can cause abnormal to the test of
1.
3-4
Chapter Three Principle
5mL
The counting pool of
Diluent RBC The count of RBC, PLT
3mL
25μL
The counting pool of
WBC The count of WBC
3.015mL
1mL
about 3ml dilution is poured at the same time to dilute the whole blood the concentration of 1:251
2. Take 25 µl dilution of blood cell 1:251 to be poured into the counting pool of RBC, into which
3. Afterwards 210 µl dilution of blood cell of 1:50,000 passes the aperture and the count of red
blood cell and platelet is carried out according to the principle of electrical impedance.
4. After 1ml lyse is added into the counting pool of white blood cell (3.0ml), the dilution is added
to be diluted to the concentration of 1:268. Then 210µl dilution of blood cell passes the aperture
and the count of white blood cell is carried out according to the principle of electrical impedance,
at the same time the photoelectric colorimetric determination of hemoglobin content is carried out
1.
3-5
Chapter Three Principle
In order to diff WBC more accurately, the precise boundary mark is used for this
instrument.
1.
3-6
Chapter Three Principle
I. Collection of Specimens
1. Choice of anticoagulant: EDTA-K2 or EDTA-K3.
2. Dosage of anticoagulant: 1.5~2.2mg/ml blood.
3. According to the dosage of anticoagulant take venous blood or capillary blood.
4. The tissue fluid may be easily mixed with the tip blood taken from earlobe or finger because of
taking blood improperly to cause the blood to be solidified and the platelet to decrease, therefore,
the blood should be mixed with anticoagulant as quickly as possible when it is taken.
5. The quantity of blood to be taken must be more than 50ul.
6. The blood should be determined after it is fully mixed.
7. It would best that the fresh blood should be determined within 15 minutes~4 hours after it is
taken.
8. After being refrigerated the anticoagulant blood may cause the white blood cell’s classification
to deviate and the histogram abnormal. After taking it out from refrigerator the blood will be
tested after it is placed 30 minutes at the normal atmospheric temperature.
Warning
● Do NOT touch the waste these materials (sample, reagent, QC substance, standard
contact with waste, immediately rinse with disinfect liquor at first and then wash with
soap completely.
● When polluted by blood, waste, please rinse with disinfect liquor, then flush with clean
● Pay more attention to that you shall wear rubber glove when deal with sample. It has
possibility of infected by pathogen. In case of it enter eyes or wound, shall rinse with
1.
3-7
Chapter Three Principle
1. Turn the test tub (bullet head) upside down, mix the blood evenly (see Fig. 1).
2. Open the lid of test tube, pay attention to not scattering the blood (see Fig. 2).
3. Place the specimen under the aspirator tip, move the specimen until the aspirator tip enters the
blood, then press down the start key (see Fig.2)
4. After the aspirator tip rises automatically, move the specimen away.
1.
3-8
Chapter Three Principle
I. Frontal plane
II Rear plane
1.
3-9
Chapter Three Principle
Keyboard: The input of letters, number and the operating control of instrument
Start key: Press down this key, the test circulation of system is started.
1.
3-10
Chapter Three Principle
1.
3-11
Chapter Four Operation Program
4-1
Chapter Four Operation Program
Examining system
Prompt
Examining system, please wait· · ·
Fig. 4-1
3) The instrument will check the switches of the electromagnetic valves, motor and pump in the
system automatically, and also use the diluent to wash the fluid pipeline and make local test
automatically, and report the result.
WBC 000.0
RBC 00.00
HGB 0000
PLT 0000
Enter
Fig. 4-2
4-2
Chapter Four Operation Program
4) Please press the “Enter” to enter main menu after background test and the screen displays
Main menu
Review
QC
Shut down
xxxx-xx-xx xx xx xx
3. Test
Press F2 test key in main menu and enter testing program, then test the daily samples.
4-3
Chapter Four Operation Program
4. Exit
1 Press F6 exit key in main menu and enter exiting program
Prompt
Shut down··
2 The circuit washing is done automatically during exiting, system will display shut down
Prompt
4-4
Chapter Five Review Record
●TEK-II Mini Analyzer has perfect function to store information and the storage is 1200
samples in which 100 samples with histograms and 1100 samples without histograms.
●All daily results can be stored automatically and the storage can be shown.
1. 3 5-1
Chapter Five Review Record
Press F1 in main menu to enter into the Record review and the screen displays:
Record review
Input date:[yy-mm-dd]
xxxx-xx-xx
1. Review Method
1 Input Date: Please input the date inquired and the screen displays:
Record review
xxxx-xx-xx
Total tests xxx
No. range xxx-xxx
Input ID
ID xxx
1. 3 5-2
Chapter Five Review Record
2. Input inquiry number: The screen displays the sample inquiried after confirming the inputted
data:
Record Review
NO 001
Item Result Unit Item Result Unit
WBC 9.3 109/L LY% 17.7 %
RBC 4.14 1012/L MONO% 3.3 %
HGB 121 g/L GR% 78.9 %
HCT 0.361 L/L LY# 1.6 109/L
MCV 87.1 fL MONO# 0.3 109/L
MCH 29.3 pg GR# 7.3 109/L
MCHC 336 g/L RDW 12.8 %
PLT 204 109/L PDW 14.7 %
MPV 12.8 fL
PCT 0.262 L/L
Diagram
Record Review
NO 001 WBC
Item Result Unit
WBC 0.0 109/L
RBC 0.00 1012/L [fl]
HGB 0 g/L 0 50 100 300 400
HCT 0.000 L/L RBC
MCV 0.0 fL
MCH 0.0 pg
MCHC 0 g/L [fl]
PLT 0 109/L 0 50 100 200
PLT
****-**-** ** **
[fl]
0 2 5 10 20 30
Next Page
1. 3 5-3
Chapter Six QC of Instrument
The aim of checking instrument’s quality is to ensure the exact and credible of the data and follow the
requirement of clinic, therefore, the instrument must proceed laboratory quality control is necessary to
control the quality of the instrument report’s result. So as to understand the condition of instrument, find out
problems; analyze reasons, take measure decrease and cancel the error in the process of test.
● Target QC
Press the quality control key F4 in main interface and enter the program of quality control, the screen
displays:
QC
Target
Float
6-1
Chapter Six QC of Instrument
I Purpose of target QC
The method of target QC is a method of QC with known target value. The QC method can detect the
instrument’s precision; at the same time, it can detect the exactness of instrument. Indicate in time whether
the calibration index is exact or not and the error of instrument is large or small.
screen displays:
WBC
xx.x xx.x
RBC
x.xx x.xx
PLT
xxx xxx
HGB
xxx xxx
MCV
xxx.x xxx.x
Figure of target QC: The tested results are vertical coordinates, the determined times are horizontal
coordinates in the figure of target QC, the central position of the vertical axis is target value ( X ), a line of
horizontal target value is drawn from that point. Then four points of up-down limits of the alarming limit of
allowable error (target value ±2SD) and maximum allowable limit (target value ±3SD) are fixed on the
vertical axis respectively, the four straight lines are drawn from the four points to parallel with the line of
target value, which is called alarming boundary line and maximum allowable boundary line respectively.
6-2
Chapter Six QC of Instrument
The target value QC is the known target value, please enter the target value firstly before quality control. The
target QC should be tested before starting operation every day to affirm whether the instrument’s condition is
normal or not.
Methods:
1. Press F1 of the target QC in the interface of quality control to enter the program of the target QC.
3. Press F3 to input new target value of the target substance and move“← →” input target value and SD
4.Press F2 and aspirator needle move down automatically for test and the screen displays:
QC test
Testing…
4.1 Overturn the test tube filled with QC substance for 20 times to mix it fully and evenly.
4.2 Open the lid of test tube and put the tube under the aspirator needle and press the start key.
4.3 The aspirator needle will move up automatically after sucking and then the substance can be moved
away.
4.4 The determined value in the picture of target QC is a vertical coordinate after the system is determined,
determined times are horizontal coordinates which draw the point of the quality control and the picture of the
quality control.
4.5 Press ESC to exit the program of target QC and then back to main interface.
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Chapter Six QC of Instrument
the test value exceeds the alarming line but in the maximum allowable boundary line, the test result is valid,
but it means that the error is larger, and please check carefully the target QC substance, reagent, instrument,
If tested value exceeds the maximum allowable boundary line, it means that it exceed control and the tested
result is invalid, and please find out the reasons of causing error, cancel it and test again.
The exactness of the determined result, the magnitude and the property of error can be analyzed and decided
6-4
Chapter Six QC of Instrument
The X B analysis carries out monitoring the determined quality of the instrument through monitoring the
stability of the average value of red cell index (MCV, MCH and MCHC) of stochastic specimen group. It is
supplemented with the quality control substance but can not replace each other.
The Purpose of using the method of floating average value method is to observe the instrument operating
condition, control the working precision in laboratory and the consistency of the test between days and
batches while detecting specimens, whether the result can be reported exactly and in time.
Press F2 floating average value key in the QC interface, the system will enter into the floating average
Float
MCV
MCH
MCHC
The decision of X B target value: Test the stochastic 500 patients continuously with the calibrated
hematology analyzer (calibrate the instrument again after determined 100 patients’ specimens), and count the
average values of red cell index, and the average value is used as the X B target value, which should be in
are 20 patients in a batch, the QC scope of the floating average value should be set up in 20 days. A
horizontal line should be drawn as target value line at the middle of vertical coordinate, the four points of
6-5
Chapter Six QC of Instrument
up-down limit of the alarming boundary of allowable error (target value ±2SD) and maximum allowable
boundary (target value ±3SD) are fixed out at the vertical axis respectively, the four straight lines are drawn
from the four points to be paralleled with the line of target value, which are called alarming boundary line
The precondition of X B analysis is to guarantee that the specimens tested by instrument is the random
sending specimens of comprehensive hospital, shall not be the classified according to the types of diseases.
If the X B analysis is made on the specimens of the pure groups of newly born babies or chemotherapies,
While the calibrated instrument test the index of red cell , the average value of the MCV, MCH and MCHC
is basically kept unchangeable no matter how many the specimens are. The change of the average value of
the parameter can be considered to be caused by instrument, reagent, operation and so on but not caused by
the change of the patients’ specimens. So the monitoring purpose can be reached through the change of the
With the floating average value QC software, the instrument can count the average value of the MCV, MCH
and MCHC with 20 specimens in one batch. The new counted data of each batch is within the established
scope of X B target value, which means the condition of instrument is fine. If it exceeds the scope, the
instrument will prompt the abnormality of instrument condition, reagent quality and operation.
If all the results are in the alarming limit in the picture of the floating average value methods, this means that
The MCHC is regarded as the most sensitive remark of losing control of the instrument. Once the MCHC
value exceeds the alarming limit in the picture of the floating average value, it means that the instrument
loses control. However, the MCV and MCH sensitivity is weak than MCHC, it is unnecessary to record the
instrument out of control, if the result exceeds 2SD a time. Only when the result exceeds 3% for 3 times or
2%-3% for 5 times continuously, it is necessary to regard the instrument is out of control. The loss of control
of MCV and MCH should not be regarded as the fault of instrument carelessly, because the counting holes
clog may also cause the loss of control of MCV and MCH, the counting aperture clog can be solved by
rinsing.
6-6
Chapter Seven Instrument Maintenance
Maintenance
Soak and rinse
Prime reagent
Setup
The system is washed perfectly with detergent about 4 minutes. Back to maintenance interface after rinsing.
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Chapter Seven Instrument Maintenance
II Aperture rinse
Press aperture rinse key F2 in maintenance interface to enter the program of aperture rinse, and the
screen displays:
Aperture rinsing
button to start
Put the concentrated detergent under aspirator needle according to the interface and press test key, the
aspirator needle will suck about 6ml liquid automatically and wash aperture again and again.
Aperture rinsing
Aperture is rinsing…
Back to maintenance interface after washing and this processing last for about 3 minutes.
7-2
Chapter Seven Instrument Maintenance
III Diagnosis
Press F3 diagnosis in the maintenance interface to enter the program of diagnosis and then the screen
Diagnosis
Valve
Ev1 Ev2 Ev3 Ev4 Ev5 Ev6 Ev7 Ev8 Ev9 Ev10
C C C C C C C C C C
Motor
M1 M2 M3 M4 M5 M6 M7
L/R U/D U/D U/D U/D O/C U/D
Entering the program can check one by one whether the electro-magnetic valve, motor, pump, switch and
HGB voltage value of the instrument are normal or not, help the users and the technicians diagnose the
operating state of each part of the instrument and to carry out the necessary maintenance .
1. Parts Diagnosis
2 The 1-10 electro-magnetic valve will carry out the change between On and Off by Pressing “Enter” to
3 To check the motor to press “Enter ” to check the function of the motor such as Up and Down, Left and
Right.
4 To check the pump to press “Enter” and the screen will display the checking processing of the pump.
Note: Each part must return the original position after carrying out diagnosis.
7-3
Chapter Seven Instrument Maintenance
1 Press F5 to confirm the voltage data and the screen will displays the device’s voltage.
3. Diagnosis Exit
Press Esc and the device will check and recover the working condition of each part automatically.
IV Prime reagents
Press F4 in the interface of prime reagent to enter the program of prime reagent and then the screen
displays:
Prime Reagent
Prime Det
Prime All
xxxx-xx-xx xx-xx
1) Prime Detergent
Press F1 of prime rinse liquid in the maintenance interface and the screen displays:
Prime Det
Priming
7-4
Chapter Seven Instrument Maintenance
Then system enters the processing of piloting rinse liquid and back to maintenance interface after finishing
the operation.
2) Prime Diluent
Press F2 of prime diluent in maintenance interface and the screen displays:
Priming Diluent
Priming
Then system enters the processing of prime diluent and back to maintenance interface after finishing the
operation.
3) Prime Lyse
Press F3 of lyse reagent in maintenance interface, and the screen displays:
Prime Lyse
Priming
7-5
Chapter Seven Instrument Maintenance
Then system carry out priming lyse and back to maintenance interface after finishing the operation.
4 Prime All
Press F4 in maintenance interface and the screen displays:
Prime All
Priming
Then system carries out priming detergent,lyse and diluent then back to maintenance interface after
7-6
Chapter Seven Instrument Maintenance
In order to keep instrument in its best state, it is necessary to maintain the instrument periodically.
pipeline automatically with the diluent, determine the blank of reagent. The normal blank of reagent is the
By turning off the machine, the system automatically rinses the counting pool, aperture and connecting
pipeline with detergent, soak the counting pool and aperture with detergent till turning on the machine
next time, this way of rinse can strongly guarantee the cleanliness and smoothness of the testing system.
1) The setup of times of continuous work: Users can set the times of continuous work according to the
condition of using the instrument, the instrument will automatically carry out washing aperture once after
reaching the set times of continuous work to insure that the instrument is used in better state.
2) Spare interval time: Users can set it according to the using condition of instrument. Instrument arrives at
the time and the system automatically carries out rinsing aperture once, then enter into the reset state to
ensure that the instrument stays in hibernate state, and the consumption of energy is reduced.
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Chapter Seven Instrument Maintenance
Warning
V. Weekly Maintenance
1. Check whether the power source and grounding wire meet the requirement or not.
2. Carry out once soak and rinse of the instrument or aperture rinse.
Use clean and wet cloth to wipe up the surface of the instrument and wipe off the bloodstain in time. Do
not wash with chemical reagent or sanding reagent. When rinsing, pull out the power supply plug, and
insert the power supply plug after instrument is clean and dry.
The instrument sets the program of system diagnosis that carries out once system diagnosis of each part
every month to ensure that each part of system can normally work..
Press F3 of system diagnosis in the maintenance interface to enter the program of system diagnosis,
which can check one by one whether the magnetic valve, motor, pump, sensor switch and HGB voltage
value of instrument are normal or not, and help users and maintenance technicians to diagnose the working
state of each part of instrument to carry out the necessary safeguard and maintenance of instrument in time.
7-8
Chapter Seven Instrument Maintenance
Warning
Warning
aperture cleaning. In the course of using the instrument, when appears the condition of high blank count,
incorrect measured value and bad repetition, and it prompts there might be half blocked aperture or
uncleanness of the tested pipeline, clean and maintain aperture and tested pipeline by the above-stated three
7-9
Chapter Seven Instrument Maintenance
When soak and rinse, system repeatedly soak and rinse the counting pool and related pipeline with 32 ml
This program is used when the dirt of the counting pool and related pipeline are difficult to be washed off.
When washing the aperture, system repeatedly rinses the aperture with 6ml concentrated liquor. This
precision of tests.
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Chapter Seven Instrument Maintenance
Hematology analyzer likes other instruments, must be calibrated with calibration substance of the known
and exact value so as to decrease the error of the system, get exact and reliable test result.
The calibration should not be done periodically. If the quality control’s data are over the normal condition
with time going, the instrument can be calibrated after instrument’s installation or maintenance. Please
Please do not calibrate it, if the abnormal data of the quality control are caused by the disturbance of
instrument, degenerative reagent and the quality control substance invalid on expiry.
1. Methods of Calibration
● Automatic calibration.
● Manual calibration.
2. Calibration Substance
1 Check whether it is over the deadline or not firstly, degenerative or polluted or not.
2 Put it for a while after taking it out from fridge, and make it reach the room temperature.
The fine condition of the instrument should be kept before calibration; it can be calibrated when the
2 The pipeline system of the instrument should be soaked and rinsed, and check whether the blank of the
3 Get a portion of fresh blood from healthy person and test it for 5-10 times, the statistical precision is
qualified.
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Chapter Seven Instrument Maintenance
4 Check the carryover rate carried by the instrument, count the carryover rate carried by the instrument to
be qualified or not.
Press F5 in maintenance interface to enter the setup program and then the screen displays:
Setup
Calibration
Time Setup
I Calibration
Press F1 of calibration in setup interface to enter the calibration program and then the screen
displays:
Calibration
●Auto Calibration ●Manual Calibration
Item Target Result Coefficient
WBC
RBC
PLT
HGB
MCV
MPV
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Chapter Seven Instrument Maintenance
Note The system will alarm if the factor is exceeded the setup range and then the change is invalid.
1 The manual calibration is applied if the determined result of calibration substance in the device is
2 Press “← →” to move cursor and input new factors and then Press “Enter”.
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Chapter Seven Instrument Maintenance
Press F2 of parameter range in set interface and enter the program of parameter range and then the screen
displays:
Range
Item min max Item min max
WBC 04.0 10.0 LY% 20.0 45.0
RBC 3.50 5.50 MID% 02.0 09.0
HGB 110 160 GR% 45.0 75.0
HCT 0.350 0.500 LY 01.0 03.3
MCV 080.0 100.0 MID 00.2 00.7
MCH 28.0 33.0 GR 01.8 06.4
MCHC 320 360 RDW-CV 11.5 14.5
PLT 100 300 RDW-SD 37 55
PDW 15.5 18.2
MPV 07.0 12.0
PCT 0.100 0.282
1. Setup Method
1 The parameter range has been set when the device is in factory, but the range is only for reference, so
2 Press “← →” to move the cursor and input the range of the clinic reference value of each parameter with
3 Press “Enter” after inputting value, and the system confirms the inputted value. If the setup is not
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Chapter Seven Instrument Maintenance
LY 1.0—3.3 109/L
LY% 20.0—45.0
Mid% 2.0—9.0
GR% 45.0—75.0
MCV 80-100 fL
MCH 28.0—33.0 Pg
RDW-CV 11.5—14.5 %
RDW-SD 37--55 fL
MPV 7.0-12.0 fL
PDW 15.5-18.2 %
There is a little difference of normal rang setup among different types, users can set the range according to
different actual conditions. If the test value is over the reference value has been set, “H” will show in the
printing result, if the value is lower, “L” will show in the printing result.
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Chapter Seven Instrument Maintenance
screen displays:
Preference
Continuous tests xx
Idle Time xx
Instrument SN xxxxxxxx
Ver. xx.xx.xx
Report + Histogram F2 On
Explanation
1.Continuous tests Use digital keys to input number in the menu of working parameter, input “↑19AB
data ↓” then “Di, Di,Di” sound will raise for three times, it means that the testing times has reached to
the setup times (max. 99 times and min. 01 times). The instrument will prompt automatically whether to
clean the aperture or not. If you do not choose, the system will impliedly carry out a aperture wash
2.Idle Time Use digital keys to input number in the menu of working parameter, input “↑29AB data ↓”
then “Di, Di,Di” will raise for three times; it means that the waiting times has reached to the setup times
(max. 99 h and min. 1 h). The instrument will carry out a blank test automatically and exit the test
5.Auto Print: Press F1 to change the mode; Press F1 and the screen displays “On”, and the result will be
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Chapter Seven Instrument Maintenance
printed automatically, if Press F1 again and the screen displays “Off”, the result will not be printed, if
6.Report + Histogram : Press F2 to change the mode; Press F2 and the screen displays “On”, and the
device will print out the histogram while print the result, if Press F2 again the screen displays “Off”, the
7.Report print format:The report print format as below.When the device connected with a computer,there
7-17
Chapter Seven Instrument Maintenance
7-18
HGB 105 g/L 110-160 PDW 16.6 % 15.5-18.2
10 20 30 fL
HCT 0.364 L/L 0.350-0.500
Chapter Seven Instrument Maintenance
IV Time Setup
Press F4 time setup key in set interface to enter the program of time setup and the screen displays:
Time Set
Date [yy-mm-dd]
xxxx-xx-xx
Time [hh-mm-ss]
xx xx xx
Note: The old ones can be replaced automatically, if input new date and time. Press “Enter” key to
7-19
Chapter Eight Analysis and Elimination of Troubles
I Background
Trouble Reason Analyzing Ways of Elimination
1. The background of a. Aperture or testing pipeline is a. Carry out the soaking and rinsing program, clean
WBC, RBC and PLT dirty. the testing pipeline and aperture of instrument.
exceed the setup range b. The reagent syringe of lyse leaks b. Carry out lyse prime to eliminate the air bubbles.
when start the gas or has air bubbles. c. Carry out a diluent prime to eliminate the air
instrument. c. The reagent syringe of diluent bubbles.
leaks gas or has air bubbles. d. Insert the pipeline under the liquor surface of
d. The pipeline of reagent did not reagent.
inserted under the liquor surface of e. Add reagent.
reagent. f. Replace the unqualified reagent with the qualified
e. Lack reagent. reagent.
f. Reagent is unqualified. g. Connect well the earth wire of instrument.
g. The earth wire with ground is in h. Move away the source of interference.
poor contact.
h. The instrument is interfered.
2. The test values of a. The lyse pipeline did not inserted a. Insert the pipette of lyse under the surface of
WBC, HGB are high under the liquor surface of lyse. liquor.
at the same time. b. Lack of lyse. b. Implement lyse.
c. EV6 Valve worked abnormal c. Rinse or replace the EV6 valve
d. Lyse nozzle blocked in WBC d. Clear block.
counting pool
3. The backgrounds of a. The aperture of RBC is dirty. a. Rinsing and wash the aperture.
RBC and PLT exceed b. The counting pipe of RBC leaks b. Connect well the counting pipe of RBC.
the setup range. gas. c. Carry out a prime diluent to eliminate air bubbles.
c. The injector of reagent leaks gas. d. Replace the unqualified lyse with qualified
d. The diluent is unqualified. diluent.
4. Only the background a. The aperture of WBC is dirty. a. Rinsing aperture, and wash the aperture.
of WBC exceeds the b. The lyse is unqualified. b. Replace the unqualified lyse with qualified one.
set range. c. The counting pipe of WBC or c. Connect well the counting pipe of WBC, carry
reagent syringe leaks gas. out a prime lyse to eliminate the air bubbles.
d. M4 injector no reverse pump d. Check or replace the M4 injector.
reactive.
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Chapter Eight Analysis and Elimination of Troubles
5. Only the background of a. The earth wire of instrument is in a. Reconnect well the earth wire.
hemoblast exceeds the poor contact. b. Move away the source of interference.
setup range. b. The instrument is interfered. c. Replace the unqualified diluent with qualified one.
c. The diluent is unqualified. d. Carry out the rinsing program of aperture.
d. The aperture is dirty. e. Replace or resolder the electrode line.
e. The electrode line of RBC
counting pool is in poor connected
or oxidated.
6. The voltage of HGB a. Lack the reagent. a. Add reagent, and restart the instrument once.
exceeds the range of b. The testing bulb of HGB burns out. b. Replace the bulb.
850-950. c. The electric eye is aging or c. Replace the electric eye.
damaged. d. Replace the TL082 Chip.
d. TL082 Chip work badness. e. Check relative pipeline
e. The liquor in counting pool of f. Tune up the HGB voltage potentiometer.
WBC is not enough.
f. The voltage excursion.
II Testing
Trouble Reason Analyzing Ways of Elimination
1. The test value of a. The aspirator needle is slightly a. Use concentrated detergent as the specimen to do several
b. The aperture of WBC is half b. Carry out cleaning gem aperture to clean it.
c. When the capacity of lyse is added out checking the switch-on and switch-off of the
more, check whether the liquor electromagnetic valve EV6, observe whether the test can
too higher or not. The normal d. Use QC blood or human being vena blood mirror
d. Not revised.
8-2
Chapter Eight Analysis and Elimination of Troubles
2. The test value of a. The end of lyse pipette is not a.Insert the pipette of lyse under the liquor surface of lyse.
WBC is high. inserted under the liquor surface of b.Add the lyse.
b. The lyse lacks. d.Replace unqualified lyse with the qualified one.
c. The aperture of WBC is dirty. e.Enter into the program of system diagnosis, repeatedly
d. The lyse is unqualified. carry out the checking of the switch-on and switch-off of
e. The proportion of diluent isn’t electromagnetic valve EV6, observe whether the test is
surface in counting pool is too low. f. Enter the program of system diagnosis, repeatedly carry
The normal liquor surface is out the checking of switch-on and switch-off of
about 5mm higher than the electromagnetic valve EV9 and EV4 observe whether the
f. The testing temperature of the g. Enter the program of system diagnosis, repeatedly carry
instrument is low. out the checking of going up and down of motor M5,
g. The earth wire doesn’t contact well observe whether the test is normal or not.
h. The instrument is interfered. i. Connect well again the earth wire of instrument.
3. The test values of a. The aperture of RBC is half a. Carry out the program of aperture and wash aperture.
RBC and PLT are blocked. b. Entering the program of system diagnosis, repeatedly
low. b. The amount of second sample is carry out the checking of going up and down of motor
not enough. M7, observe whether the test can be normal or not.
c. RBC counting pool vent liquor c. Entering the program of system diagnosis, repeatedly
be normal or not.
RBC and PLT are b. The aperture of RBC is dirty. b. Carry out the cleaning gem aperture, clean the gem
correct, and check whether the c. Enter the program of system diagnosis, repeatedly carry
liquor surface in counting pool of out the checking of switch-on and switch-off of the
RBC is too low or not. (The normal electromagnetic valve EV9, EV4 and EV5, observe whether
liquor surface is about 5mm higher the test can be normal or not .and carry out the checking of
the submerged aperture. going up and down of the motor M5, observe whether the
8-3
Chapter Eight Analysis and Elimination of Troubles
RBC and HGB are b. The placement of diluent is too b. Heighten the place of diluent and the level of instrument.
c. The voltage of HGB is abnormal. HGB voltage is between 850-950 or not. otherwise, tune
6. The test value of a. The test voltage of HGB is a. Replace the luminotron of HGB and electric eye.
HGB is zero or abnormal. b. Carry out soaking and rinsing once, clean the counting
c. The lyse is unqualified. c. Enter the program of diagnosis, repeatedly carry out the
pool of WBC is not better. electromagnetic valve. and carry out the checking of going
electromagnetic valve.
7. The test values of a. The voltage of HGB is checked to a. Replace the luminotron of HGB.
WBC, RBC and be abnormal. b. Carry out soaking and rinsing once, clean the counting
PLT are normal, b. The counting pool of WBC is dirty. pool of WBC.
displays zero.
8. The test values of a. The proportion of diluent is not a. Enter the program of system diagnosis, repeatedly check
WBC, RBC and correct, check whether the liquor the switch-on and switch-off of the electromagnetic valve
PLT are all surfaces in two counting pools of EV9, EV8, EV4, EV5 and EV7, observe whether the test
unstable. blood cell are abnormal or not. can be normal or not. Entering the program of system
The normal liquor surface is diagnosis, repeatedly carry out the checking of going up
about 5mm higher than the and down of motor M5, observe whether the test can be
b. The counting time is unstable. b. Check whether the counting syringe works normally or
d. The earth wire of instrument falls c. Carry out the program of aperture wash, and wash the
off. aperture.
8-4
Chapter Eight Analysis and Elimination of Troubles
9. The test value of a. The aperture of RBC is half a. Carry out the program of aperture wash and wash the
unstable. b. The earth wire of instrument does b. Connect well the earth wire of instrument.
10. The test values of a. The aperture of RBC is blocked. a. Wash the aperture.
RBC and b. The connecting pipe of counting b. Connect well the counting pipeline.
hemoblast are all syringe of RBC falls off. c. Repeatedly carry out the checking of going up and down
sample is abnormal.
11. The test value of a. The aperture of WBC is blocked. a. Wash the aperture
WBC is zero. b. The connecting pipe of counting b. Connect well the counting pipeline.
12. Aspirator needle a. The Aspirator needle is blocked. a. Using the rinse liquid instead of specimen, continuously
doesn’t suck the b. The pipe of specimen sucking leaks determine several times.
specimen or the gas. b. Check the leaking part and connect well the related
sucked specimen is c. The aspirator needle doesn’t pipeline. Check whether the upper end of connecting pipe
not enough. completely insert into the of aspirator needle falls off or not.
d. M4 motor can not work normally. d. Entering the program of diagnosis, repeatedly carry out
III Drainage.
8-5
Chapter Eight Analysis and Elimination of Troubles
1.The liquor in counting a. The electromagnetic valveEV5 a. Entering the program, repeatedly carry out
pool of WBC can not be can not work normally. checking switch-on and switch-off of valve EV5,
drained completely. b. The pump of liquor drainage can observe whether the liquor drainage can be normal
2. The liquor in counting a. The electromagnetic valveEV7 can a. Entering the program of diagnosis, repeatedly
pool of RBC can not be not work normally. carry out checking switch-on and switch-off of
drained completely. b. The pump of liquor drainage can valve EV7, observe whether the liquor drainage
switch-off.
3. WBC, counting pool of a. The pump of liquor drainage can a. Entering the program of diagnosis, repeatedly
RBC and waste liquor not work normally. carry out checking switch-on and switch-off of
can not be drained b. The electromagnetic valve EV5 or pump of liquor drainage, observe whether the
completely at the same EV7 can not work normally. liquor drainage can be normal or not.
time. c. Buffer bottle leaks gas. b. Entering the program of diagnosis, repeatedly
IV Feeding
8-6
Chapter Eight Analysis and Elimination of Troubles
1. The liquor can not enter a. The reagent pipette doesn’t insert a. Insert the reagent pipette under the surface of
into the counting pool. under the surface of reagent reagent liquor.
c. The reagent syringe can not work out checking going up and down of motor M5 and
not normally open and close. d. Entering the program of diagnosis, repeatedly carry
e. The liquor pipeline falls off. out checking the switch-on and switch-off of the
well again.
2. The liquor can not enter a. The electromagnetic valve can a. Entering the program of diagnosis, repeatedly carry
the counting pool of not work normally. out checking the switch-on and switch-off of valve
WBC. b. EV5 on the bottom of counting EV4, EV8 and EV9, observe whether the liquor can
into or not.
3. The liquor can not enter a. The electromagnetic valve can a. Entering the program of diagnosis, repeatedly carry
the counting pool of not work normally. out checking the switch-on and switch-off of valve
RBC. b. EV7 on the bottom of counting EV4, EV8 and EV9, observe whether the liquor can
into or not.
V Alarm
Alarm Reason Ways of Elimination
8-7
Chapter Eight Analysis and Elimination of Troubles
1. M1 or M2 can not move a. The motor can not move to the a. Readjust or lubricate.
to the position. position or the resistance of motion b. Power supply wiring of motor falls off or the motor
is big. is damaged.
b. The motor can not work. c. Check whether the photoelectricity or the jiggle
2. M3 can not move to the a. The motor can not move to the a. Readjust or lubricate.
position. position or the resistance of motion b. Power supply wiring of motor falls off or the motor
is big. is damaged.
b. The motor can not work. c. Check whether the photoelectricity is failure.
3. M4 can not move to the a. The motor can not move to the a. Readjust or lubricate.
position. position or the resistance of motion b. Power supply wiring of motor falls off or the motor
is big. is damaged.
b. The motor can not work. c. Check whether the photoelectricity is failure.
4. M5 can not move to the a. The motor can not move to the a. Readjust or lubricate.
position. position or the resistance is large. b. Power supply wiring of motor falls off or the motor
failure.
5. M7 can not move to the a. The motor can not move to the a. Readjust or lubricate.
position. position or the resistance is large. b. Power supply wiring of motor falls off or the motor
failure.
VI Printing
VII Display
8-8
Chapter Eight Analysis and Elimination of Troubles
1. Blue screen a. The potentiometer not be tuned a. Tune up the potentiometer to the correct position.
Note: The troubles explained in this instructions can be eliminated according to the instructions as for
the unmentioned troubles in the instruction, please contact our company or the agents.
8-9
Reference I Online Application Software Instruction
Reference I
II. CD Installation
1. win98.winme OS
a) Run setup.exe program in “Accessories” folder firstly. It maybe appears the prompt:”
Whether replace it” when starting install this program. Please click “No”. If it appears
“Ignore”, Please click “Ignore”.
b) Run setup.exe in “Installation package”
2. winxp OS
Run setup.exe in “Installation package”.
If it be changed, please modify the name into “Mini_sy.MDB”. If it doesn’t work yet, please copy
“PC_M” into “C:” partition root directory(not recommend). But the files propertys in “PC_M”
folder needed to be changed after copying finished. Modify the read only property into written.
There are 90 days trial after installation. The “Hospital name” in “work parameters” can be revised
so as to use more convenience. It is needed to inform the distributor the “Hospital name” in “work
patameters” firstly when you want to register this program. Dealer will give you a validation code.
Then this program can be used directly after entering this code into “work parameters”. Otherwise
it can’t work when exceeded the trial time.
NOTICE: The hospital name can’t be changed informally. Otherwise this grogram can’t be run
normally.
TECOM CHINA
Reference I Online Application Software Instruction
IV Edition Instruction
You can obtain the software edition from “Work Parameters”. Different edition match different
object.
TECOM CHINA
Reference I Online Application Software Instruction
I. Data Receiving
Click the receiving key in main menu(or press F3),then the testing interface comes out.. Start test
the specimen after enter the samples data. The Screen prompt receiving data after test complete.
Test result can be shown on the screen about 3 seconds later. Click print button(or press F4) to print
the result.
Attention: Computer only get the data which being tested and get it when the device and
computer both in test program interface. Otherwise the result can’t be received.
Click the assay form edit button in main menu (or press F5).Enter in edit interface. Click add button.
Then the samples data can be edited.
IV Setup
Click setup button in Main Menu(or press F6),Enter in setup interface. This menu include: Pa
rameter Range, Work Parameter, Print Setup, User Setup, Doctor Setup, Dept. Setup.
1.Parameter Range: This function is about various parameter range of blood in human body.
2.Work Parameter: Include Hospital Name, Installation validation code, Print Setup, Time Setup
and Edition information.
3.Print Setup: Include various print format, chose by user.
4.User Setup: Can add and delete user account in this item. But only superpower user can change.
5.Doctor Setup: Can add and delete doctor’s name.
6.Dept. Setup: Can add and delete department name.
V Record Inquiry
Click Record Inquiry button in Main Menu (or press F7).In Record Inquiry menu. enter
the inquiry date or patient name according to the samples for searching. All information received
by computer can be searched in this record.
VI QC
Details in Chapter Six.(This application added non-target QC).The device test QC substance in
Test interface. Data can be received only by computer shows the test interface and at the meantime
TECOM CHINA
Reference I Online Application Software Instruction
VIII Exit
Click Exit, interface prompt confirm frame. Click Yes to exit. Click Cancel to enter in login
interface. So that another doctor can login.
TECOM CHINA
Reference II
1. Hardware connection: use direct series line to connect (2 pin to 2 pin,3 pin to 3 pin)
2. Series port setup: 19200,n,8,1
3. Communications
Contact Character ‘U’ Within 150ms PC send back ‘S’ available. Separate data with “#”
Item Type ‘8’(or‘9’) # ‘8’ ÆItem 18 ,‘9’ÆItem 19. Use other number for future expanding
Number ***#
WBC Histogram 125 **#
RBC Histogram 125 **#
PLT Histogram 125 **#
18 or 19 Items data See data format below
2 RBC **.**#
3 HGB ****#
4 HCT *.***#
5 MCV ***.*#
6 MCH **.*#
7 MCHC ****#
8 PLT ****#
9 LY% **.*#
10 MID% **.*#
11 GR% **.*#
12 LY# ***.*#
13 MID# ***.*#
14 GR# ***.*#
Reference II
15 RDW-CV **.*#
16 RDW-SD **.*#
17 MPV **.*#
18 PCT *.***#
19 PDW **.*#
Transportation
No. Item Instruction
Data Format
Index
A
Aperture Rinse…………………………………………………………………………………7-2
Analysis and Elimination of Troubles…………………………………………………………8-1
Alarm………………………………………………………………………………………… 8-7
B
Background……………………………………………………………………………………8-1
Basic Parameters of Instrument………………………………………………………………2-4
C
Check before Testing…………………………………………………………………………4-1
Connection of pipeline…………………………………………………………………………1-2
Calculation Parameter…………………………………………………………………………3-2
Calibration………………………………………………………………………………………7-12
Calculation of WBC classification………………………………………………………………3-6
Counting Principle of Blood Cell………………………………………………………………3-1
Collection of Specimens…………………………………………………………………………3-7
Collection and Measurement of Specimens ……………………………………………………3-7
D
Drainage…………………………………………………………………………………………8-5
Daily Operation…………………………………………………………………………………4-1
Daily Maintenance………………………………………………………………………………7-7
Diagnosis…………………………………………………………………………………………7-3
Diagnosis and Exit………………………………………………………………………………7-4
Testing of Specimen………………………………………………………………………………3-8
E
Empty the Waste Liquor Barrel…………………………………………………………………7-7
Establishment of WBC Classification…………………………………………………………3-6
Environment of Instrument……………………………………………………………………4
F
Film Keyboard instruction………………………………………………………………………2-6
Feeding……………………………………………………………………………………………8-6
Figure of QC……………………………………………………………………………………6-2
Index
G
General Instruction……………………………………………………………………………2-2
Grounding………………………………………………………………………………………1-1
General Outline of Instrument…………………………………………………………………2-1
General Parameter Range Supplied by Device…………………………………………………7-15
H
HGB Voltage Measurement………………………………………………………………………7-4
I
Installation ………………………………………………………………………………………1-1
Implementation of the Target QC’s Program……………………………………………………6-3
Introduction………………………………………………………………………………………2-1
Inspection Principle………………………………………………………………………………3-1
Introduction of Fluid System……………………………………………………………………3-5
Introduction of technological process……………………………………………………………3-5
Instrument Maintenance…………………………………………………………………………7-1
In Main Menu……………………………………………………………………………………2-6
M
Menu of Tree Structure…………………………………………………………………………2-5
Methods of Calibration…………………………………………………………………………7-11
Monthly Maintenance……………………………………………………………………………7-8
Main interface……………………………………………………………………………………2-6
N
Necessary Maintenance…………………………………………………………………………7-9
O
Operating Program………………………………………………………………………………4-1
Operating Interface………………………………………………………………………………2-6
P
Parameter Calibration…………………………………………………………………………7-14
Parameter Range Setup………………………………………………………………………7-14
Parameter Instruction…………………………………………………………………………2-4
Printer…………………………………………………………………………………………1-2
Power Regulate…………………………………………………………………………………1-1
Purpose of Target QC…………………………………………………………………………6-2
Picture of Method of the Floating average value………………………………………………6-5
Index
Q
Quality Judgement of the Method of the Floating Average Value…………………………6-6
Quality Control of Instrument………………………………………………………………6-1
Quality Judgment of the Quality Control……………………………………………………6-3
R
Record review………………………………………………………………………………5-1
Resistance Protecting Principle……………………………………………………………3-1
Reagent,QC substance and Standard Substance……………………………………………2-7
Reagent pack and deadline…………………………………………………………………2-10
S
Safe for use ………………………………………………………………………………5
Shut down…………………………………………………………………………………4-4
Soak and Rinse……………………………………………………………………………7-1
Starting the machine………………………………………………………………………4-1
Samples Testing……………………………………………………………………………4-1
Setup………………………………………………………………………………………7-11
Structure of External Part…………………………………………………………………3-10
Setup of Calibration…………………………………………………………………………7-12
T
Time setup…………………………………………………………………………………7-19
Test…………………………………………………………………………………………4-3
Testing Program……………………………………………………………………………4-3
Testing Interface……………………………………………………………………………2-7
Target QC……………………………………………………………………………………6-2
Testing………………………………………………………………………………………8-2
The permissive blank value of reagent………………………………………………………2-11
The testing of HGB……………………………………………………………………………3-2
Index
W
Way of Inquiry………………………………………………………………………………5-3
Work Parameter setup………………………………………………………………………7-16
Weekly Maintenance…………………………………………………………………………7-8
Product name Full auto 3-diff hematology analyzer