CATUDIO, FRANCIS JOSEPH B

BIO 191 Introduction to Genomics and Bioinformatics

HYDROSTATIC GENE TRANSFER C E L L S Q U E E Z E

 The cell is allowed to flow in a device, called as the

microfluidic delivery platform.
 As the cells move through the device, the channel
width narrows until a cell finds itself in a tight spot.
 The cell membrane will change in form in order
for the cell to fit into the narrow channel.
 The forced, rapid change in cell shape creates
temporary holes in the membrane without
damaging or killing the cell
 DNA is now then allowed to enter the cell through
the pores created from the rapid change in cell
shape.
 Hydrodynamic force generated by a pressurized  As the cell returns to its original shape, the pores
Injection of large volume of DNA is applied into slowly closes and the DNA that entered it are
the blood vessel trapped inside.
 Capillary endothelium is permeabilized and pores ADVANTAGES DISADVANTAGES
are generated - It allows transfer of molecules
 DNA enters the parenchyma cells through the without damaging or killing the cell (as
pores compared to electroporation)
 With time, the membrane pores close, trapping - it can be used in a wide variety of
the DNA inside. animal cells, including those that are
fragile to the current transfection
ADVANTAGES DISADVANTAGES methods
- An effective tool for - not feasible in humans -large molecules are allowed to enter
establishing disease in model since it may induce the cell
animals irregularity in cardiac
- More gene product are function
produced as compared to
intramuscle injection of References
plasmid DNA Johnson, T. (2013, June 3). Researchers Put Squeeze On Cells
- The use of animal system as
To Deliver. Retrieved from National Institute of
compared to bacteria or
yeast system is that Biomedical Imaging and Bioengineering:
expression of transgene in https://www.nibib.nih.gov/news-
rodents avoids the problem events/newsroom/researchers-put-squeeze-cells-
of misfolding and improper deliver
glycosylation
Suda, T., & Dexi, L. (2007). Hydrodynamic Gene Delivery: Its
Principles and Applications. Cell Press, 2063-2069.