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Mouse IgG2a kappa Isotype Control, PE,

eBioscience™
Catalog Number 12-4724-41 Product data sheet

Details Species Reactivity

Size 25 Tests Species reactivity Not Applicable

Host/Isotope Mouse / IgG2a, kappa Tested Applications Dilution *

Class Control Control (Ctrl) Assay-Dependent
Type Isotype Control Flow Cytometry (Flow) 5 µL (0.5 µg)/test

Clone eBM2a Published Applications

Target Class IgG2a Flow Cytometry (Flow) See 11 publications below
Conjugate PE Control (Ctrl) See 2 publications below

Form Liquid * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment
using appropriate negative and positive controls.
Concentration 5 µL/Test
Purification Affinity chromatography
PBS, pH 7.2, with 0.1% gelatin, 0.2%
Storage buffer
BSA
Contains 0.09% sodium azide
4° C, store in dark, DO NOT
Storage Conditions
FREEZE!

Product specific information

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This
mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis. Applications Tested: This Mouse IgG2a K
Isotype Control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at the
same concentration as the experimental antibody.- test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood
cells. This can be used at test size: 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100
µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser,
Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

Background/Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be
caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence.
Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have
no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as
chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so
that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works
best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.

NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.

Thermo Fisher Scientific

10255 Science Center Drive
San Diego, CA 92121
PubMed References For Mouse IgG2a kappa Isotype Control, PE, eBioscience™
11 Flow Cytometry References
Species / Dilution Summary
12-4724 was used in Flow cytometry/Cell sorting to demonstrate that L. casei modulates the interaction between IECs and
immune cells during the immune response.

Veterinary research (Nov 2011; 42: null)

Not Applicable / Not Cited
"Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal
Pig / Not Cited epithelial cells."
Author(s):Hosoya S,Villena J,Shimazu T,Tohno M,Fujie H,Chiba E,Shimosato T,Aso H,Suda Y,Kawai Y,Saito T,Alvarez S,
Ikegami S,Itoh H,Kitazawa H
PubMed Article URL:http://dx.doi.org/10.1186/1297-9716-42-111
12-4724 was used in Flow cytometry/Cell sorting to study carotenoid activity via biologically active polymers from
spontaneous carotenoid oxidation.
Not Applicable / Not Cited
PloS one (Jun 2015; 9: null)
Human / Not Cited "Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity."
Author(s):Johnston JB,Nickerson JG,Daroszewski J,Mogg TJ,Burton GW
PubMed Article URL:http://dx.doi.org/10.1371/journal.pone.0111346
12-4724 was used in Flow cytometry/Cell sorting to identify, pheontype, and assess endocytic ability of peripheral blood
dendritic cell subsets in a single platform using an 11-color flow cytometric assay.

Not Applicable / Not Cited Journal of immunological methods (Feb 2009; 341: 106)
Human / Not Cited "An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood
dendritic cell subsets in a single platform."
Author(s):Wang JC,Kobie JJ,Zhang L,Cochran M,Mosmann TR,Ritchlin CT,Quataert SA
PubMed Article URL:http://dx.doi.org/10.1016/j.jim.2008.11.002
12-4724 was used in Flow cytometry/Cell sorting to study the molecular mechanisms behind primary human lung cancer
outgrowth in response to LPS.

Oncotarget (Jul 2016; 7: 42172)

Human / Not Cited
"TLR4/ROS/miRNA-21 pathway underlies lipopolysaccharide instructed primary tumor outgrowth in lung cancer
patients."
Author(s):Zhang X,Wang C,Shan S,Liu X,Jiang Z,Ren T
PubMed Article URL:http://dx.doi.org/10.18632/oncotarget.9902
12-4724 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma
brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.
Not Applicable / Not Cited
PLoS pathogens (Jul 2016; 12: null)
Mouse / Not Cited "Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells."
Author(s):Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ
PubMed Article URL:http://dx.doi.org/10.1371/journal.ppat.1005733
12-4724 was used in Flow cytometry/Cell sorting to show that JQ1 (a BET inhibitor) potently reduces the viability of B-ALL cell
lines with high-risk cytogenetics.

Human / Not Cited Blood (Oct 2012; 120: 2843)

"BET bromodomain inhibition targets both c-Myc and IL7R in high-risk acute lymphoblastic leukemia."
Author(s):Ott CJ,Kopp N,Bird L,Paranal RM,Qi J,Bowman T,Rodig SJ,Kung AL,Bradner JE,Weinstock DM
PubMed Article URL:http://dx.doi.org/10.1182/blood-2012-02-413021
12-4724 was used in Flow cytometry/Cell sorting to prove that single-agent chemotherapy can eradicate large, established
tumours and induce long-term immune memory.

Mouse / Not Cited Oncoimmunology (Apr 2015; 4: null)

" T-cell responses and immune memory."
Author(s):Wu J,Waxman DJ
PubMed Article URL:http://dx.doi.org/10.1080/2162402X.2015.1005521

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.

NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.

Thermo Fisher Scientific

10255 Science Center Drive
San Diego, CA 92121
 12-4724 was used in Flow cytometry/Cell sorting to statistically analyse miRNA microarray data, revealing that guanine-rich
sequences are a dominant feature of exosome-dominant miRNAs.

Not Applicable / Not Cited PloS one (Apr 2017; 11: null)
Human / Not Cited "Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell
Types."
Author(s):Momose F,Seo N,Akahori Y,Sawada S,Harada N,Ogura T,Akiyoshi K,Shiku H
PubMed Article URL:http://dx.doi.org/10.1371/journal.pone.0154134
12-4724 was used in Flow cytometry/Cell sorting to investigate the conditions contributing to the development of CIDP and
related conditions.

Not Applicable / Not Cited PloS one (Sep 2009; 4: null)

Mouse / Not Cited "Interleukin-10 overexpression promotes Fas-ligand-dependent chronic macrophage-mediated demyelinating
polyneuropathy."
Author(s):Dace DS,Khan AA,Stark JL,Kelly J,Cross AH,Apte RS
PubMed Article URL:http://dx.doi.org/10.1371/journal.pone.0007121
12-4724 was used in Flow cytometry/Cell sorting to investigate disruption of immune silencing by proteinase 3 on apoptotic
cells.

Not Applicable / Not Cited The Journal of clinical investigation (Nov 2015; 125: 4107)
Human / Not Cited "Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis."
Author(s):Millet A,Martin KR,Bonnefoy F,Saas P,Mocek J,Alkan M,Terrier B,Kerstein A,Tamassia N,Satyanarayanan SK,Ariel
A,Ribeil JA,Guillevin L,Cassatella MA,Mueller A,Thieblemont N,Lamprecht P,Mouthon L,Perruche S,Witko-Sarsat V
PubMed Article URL:http://dx.doi.org/10.1172/JCI78182
12-4724 was used in Flow cytometry/Cell sorting to describe a role for host caspase-1 and ASC and the parasite GRA15
protein in Toxoplasma gondii-induced IL-1 production.

Human / Not Cited mBio (Jul 2013; 4: null)

"Human innate immunity to Toxoplasma gondii is mediated by host caspase-1 and ASC and parasite GRA15."
Author(s):Gov L,Karimzadeh A,Ueno N,Lodoen MB
PubMed Article URL:http://dx.doi.org/10.1128/mBio.00255-13
2 Control References
Species / Dilution Summary
12-4724 was used as a Control in experiments to investigate the role of TMED7 in the regulation of TLR4 signalling.

Science signaling (Jul 2014; 7: null)

Human / Not Cited "The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane."

Author(s):Liaunardy-Jopeace A,Bryant CE,Gay NJ

PubMed Article URL:http://dx.doi.org/10.1126/scisignal.2005275
12-4724 was used as a Control in experiments to discover that the PDCD-1 polymorphism rs36084323 was significantly
associated with rheumatoid arthritis risk in Han Chinese population.

International journal of genomics (May 2014; 2014: null)

Human / Not Cited
"A Promoter Region Polymorphism in PDCD-1 Gene Is Associated with Risk of Rheumatoid Arthritis in the Han
Chinese Population of Southeastern China."
Author(s):Liu C,Jiang J,Gao L,Hu X,Wang F,Shen Y,Yu G,Zhao Z,Zhang X
PubMed Article URL:http://dx.doi.org/10.1155/2014/247637

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.

NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.

Thermo Fisher Scientific

10255 Science Center Drive
San Diego, CA 92121