Mesenchymal Stem Cell-Conditioned Medium with Serum Injured

Accelerates Wound Healing in Full-Thickness Skin Defect Model

Agung Putra1,2,3, Durotul Jannah4, Fadhli Rizal Makarim1, Rita Aryanti1, Citra
Desi Dariya5, Rizky Aslia Kusuma Putra5, Ade Yorga Tonara5, Azizah Retno
Kustiyah6

1. Stem Cell And Cancer Research (SCCR), Medical Faculty, Universitas Islam
Sultan Agung (UNISSULA), Semarang, Indonesia.
2. Departement of Pathological Anatomy, Medical Faculty, Universitas
Universitas Islam Sultan Agung (UNISSULA), Semarang, Indonesia.
3. Department of Postgraduate Biomedical Science, Faculty of Medicine,
Universitas Islam Sultan Agung (UNISSULA), Semarang, Indonesia
4. Department of Neurology of Medical Faculty, Universitas Islam Sultan Agung
(UNISSULA), Semarang, Indonesia
5. Undergraduate Program of Medical Faculty, Universitas Islam Sultan Agung
(UNISSULA), Semarang, Indonesia
6. Departement of Pediatric of Medical Faculty, Universitas Islam Sultan Agung
(UNISSULA), Semarang, Indonesia
Correspondence: Dr. dr. H. Agung Putra,M. Si. Med., Chairman of Stem Cell and
Cancer Research (SCCR) Laboratory, Medical Faculty, Universitas Islam Sultan
Agung Semarang, Jl. Raya Kaligawe KM. 4 Semarang, Jawa Tengah 50112 Phone.
+628164251646 Email: dr.agungptr@gmail.com

Acknowledgments: We would like to thanks to Stem Cell And Cancer Research

Labolatory, Medical Faculty, Universitas Islam Sultan Agung Semarang for all
Facility to finish this research.
 ABSTRACT
Background: Umbilical cord-mesenchymal stem cells (UC-MSCs) accelerating
wound closure by increasing VEGF and PDGF level leading to re-epithelialization,
cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium
(MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the
effect of MSC-CM with Serum Injured in accelerating wound healing remains
unclear. This study aims to evaluate effect of several doses topical MSC-CM in
accelerating wound healing.
Method: Eighteen Wistar rats were randomly assigned into vehicle group (Veh),
treatment group (T1, T2) and were received serum-free gel (Veh), MSC-CM gel 1:5
(T1), MSC-CM gel 1:10 (T2) respectively, twice daily for 9 days. PDGF and VEGF
level measured using ELISA on day 3 and 6.

Result: This study showed a significant increase in VEGF and PDGF level (p<0.05)
on day 3 and 6, along with a significant increase (p<0.05) in fibroblast density. 

Conclusion:   In   conclusion,   topical   gel   with   high   concentration   of   MSC­CM   is

effective to accelerate wound closure by enhancing PDGF and VEGF level in full­
thickness skin defect rats compared to control group. 

Keyword: Fibroblast, PDGF, UCMSCs­CM, VEGF, Wound healing