‘om Pharmaceuti Pharmaceutical Analysis 46 Analysis Questions Alerts! Common questions in pharmacy exam is to ask! * Chromatography methods like HPLC, GC, TLC * Spectroscopic methods like mass spectroscopy © Bioassays like ELISA, gel electrophoresis western blot, Tips infection 1. | todry wet 2. | the method of ionization of | 3. | purification and analysis powders, which are MS w/c results in well- of pharmaceuticals heat sensitive established fragmentation drugs pattern that are useful in identification of unknown 4. | Analysis and protein separation 6. | protein isolation detection of volatile chemical in samples [7 [enzyme linked 8. | HPLC 9. | UV, DAD, Fluorescent & | | immunosorbent RI assay 10. | Gas 11. | capillary action 12. | amine, amino sugars and Chromatography proteins 13. | Increase in solubility | 14. | Decrease in solubility by | 15. | Detectors, printers by addition of salt addition of salt 16.| Detection of HIV | 47. | Mass Spectroscopy Pump is present in what type of chromatography? (HPLC) Types of detectors used in HPLC? (UV, DAD, Fluorescent and Rl) Drugs present in blood samples can be detected by? (MS) Copyright © 2000-2012 TIPS Inc. Unauthorized reproduction of this manual is prohibited. This manual is 46-1 being used during review sessions conducted by PharmacyPrep.www, rep.com Pharmaceutical Analysis © Salting in is? (Increase in solubility by addition of salt) ‘© Salting out is? (Decrease in solubility by addition of salt) '* Mechanism of TLC is based on? (Capillary action) ‘* Niphydrin is used to detect? (Amines, amino acids, amino sugars, and proteins) ‘+ What are the factors that does not affect resolution in HPLC? (Detectors and printers) GC-MS is? ( Gas chromatography and mass spectrometry) + Lyophilization (Freezing) is used for? (heat labile products) Electron impact (E!) ionization mass spectroscopy is? (Molecular ion detection) HPLC is used for? (Purification and analysis) ELISA is? (enzyme linked immunosorbent assay) ELISA is used for? (to diagnose AIDS) + Western blot test for? Nucleic acid (DNA and RNA) analysis * Gel electrophoresis separates? (proteins) Copyright © 2000-2012 TIPS Inc. Unauthorized reproduction ofthis manual is prohibited. This manual is 46-2 ‘being used during review sessions conducted by PharmacyPrep,Social/Behavioral and Administrative Sources Part Social / Behavioral and Administrative Sciences Copyright © 2000-2012 TIPS Ine. Unauthorized reproduction ofthis manual s prohibited. This manual is 47] being used during review sessions conducted by PharmacyPrep.