Combatir, Kaori Maire S., and Velasco, Kye Alexandrea J.

,
“Evaluation of Antimitotic Activity of Carrot (Daucus carota)
Peel Extract Using Onion (Allium cepa L.) Root Assay”
Unpublished Undergraduate Thesis, Bachelor of Science in
Biology, Premedicine track, College of Arts and Sciences, West
Visayas State University, La Paz, Iloilo City.

Abstract

This study aimed to test the antimitotic activity of carrot

peel extract using onion root assay based on percent

inhibition, mitotic index, and median mitotic inhibition. The

setups consisted of positive control (Alpha Lipoic acid at

0.1mg/ml), negative control (Cyclophosphamide at 0.1mg/ml) and

experimental groups (carrot peel extract at 10 mg/ml, 20mg/ml

ang 30mg/ml + Cyclophosphamide at 0.1mg/ml each). Onion root

cells exposed to 3 carrot peel extract concentrations did not

produce aberrant chromosomes. Chromosomal aberrations were

found on cyclophosphamide which include polyploidy and sticky

metaphase, anaphase with bridge, sticky anaphase, vagrant

chromosome in telophase and disturbed telophase. Chromosomal

aberrations found on alpha lipoic acid were anaphase with

bridge and disturbed telophase. The 10% CPE + cyclophosphamide

treatment has the only positive mean value for the mitotic

inhibition.One-Way multivariate analysis of variance showed no

significant difference on the on mitotic inhibition and

mitotic index among the treatments used, p>0.05. Thus, at

various concentrations of CPE extract may possess the

protective mechanism of producing chromosomal damage.