Method Validation of the UV Spectrophotometric Method for the Determination of Benzoic Acid in

Data on the UV Spectra of the Blank, Standard Benzoic Acid, & Sample Solutions 1st Meeting
Solution λmax, nm
Blank ---
Standard Benzoic 231.0 explain how your data spectro affect your data
Caffeine Sample 273.0

Data on the UV Absorbance of Standard Benzoic Solutions (w/o Caffeine) 1st meeting
Absorbance
Conc., mg/L

0 -0.0040
2 0.0010
4 0.0150
6 0.0300
8 0.0460
10 0.0620

Data on the UV Absorbance of Standard Benzoic Solutions (w/o Caffeine) 2nd Meeting
Absorbance
Conc., mg/L

0 -0.0040
2 0.0460
4 0.0820
6 0.1310
8 0.1710
10 0.2100

Data on the UV Absorbance of Standard Benzoic Solutions (w/ Caffeine) 1st meeting
Absorbance 0.0111g/100 ml h20 - stock
Conc., mg/L

0 -0.004
2 0.0770
4 0.1340
6 0.2050
8 0.2800
10 0.3380
mination of Benzoic Acid in Soft Drinks

Meeting

0.0700
0.0600
f(x) = 0.0068571429x - 0.0092857143
Linear Analysis of Benzoic Acid 0.0500 R² = 0.9819297647
Conc., Ave. Calibration Curve w/ HCl

Absorbance
0.0400
mg/L Absorbance Parameters Values
0.0300
0 -0.0040 slope 0.0068571429
0.0200
2 0.0010 y-int -0.0092857143
0.0100
4 0.0150 r^2 0.9819297647
6 0.0300 0.0000
0 2 4 6
8 0.0460 -0.0100
10 0.0620 w/o Caffeine Concentration,

0.2500
Linear Analysis of Benzoic Acid
0.2000 f(x) = 0.0213428571x - 0.0007142857
Conc., Ave. Calibration Curve w/ HCl R² = 0.9982539782
mg/L Absorbance Parameters Values Absorbance 0.1500
0 -0.0040 slope 0.0213428571
0.1000
2 0.0460 y-int -0.0007142857
4 0.0820 r^2 0.9982539782 0.0500
6 0.1310
0.0000
8 0.1710 0 2 4

10 0.2100 -0.0500

w/o Caffeine Concentrati

Linear Analysis of Benzoic Acid

Conc., Ave. Calibration Curve w/ HCl
Parameters Values 0.4000
mg/L Absorbance
0 -0.0040 slope 0.0341428571 0.3500
f(x) = 0.0341428571x + 0.000952381
2 0.0770 y-int 0.000952381 0.3000 R² = 0.9981419135
4 0.1340 r^2 0.9981419135 0.2500
Absorbance

6 0.2050 0.2000
8 0.2800 0.1500
10 0.3380 0.1000
0.0500
0.0000
0 2 4 6
-0.0500
Concentration, m
w/ Caffeine
0.0500
0.0000
0 2 4 6
-0.0500
Concentration, m
w/ Caffeine
29x - 0.0092857143
7

4 6 8 10 12

Concentration, mg/L

428571x - 0.0007142857
39782

2 4 6 8 10 12

Concentration, mg/L

71x + 0.000952381
5

4 6 8 10 12

Concentration, mg/L
4 6 8 10 12

Concentration, mg/L
 Method Validation of the UV Spectrophotometric Method for the Determination of Benzoic Acid in So

Data on the UV Absorbance of the Method Blank Sample Solutions 2nd Meeting (0.1 ppm)
Replicate Absorbance
1 -0.001 calib curve from 2nd meeting
2 0.004 slope perform
0.0213429
test for outliers, compute necessarry param
3 -0.001 y-int -0.000714
4 0.003 r^2 0.998254
5 0
6 0.002
7 -0.001
8 -0.002
9 -0.002
10 0.001

sd 0.0021108187
mean 0.0003
LOD 0.0063324561
LOQ 0.0211081869
abs conc
mean+3sd 0.0066324561 0.3442248
mean+10sd 0.0214081869 1.0365282
nation of Benzoic Acid in Soft Drinks

rs, compute necessarry parameters

 Method Validation of the UV Spectrophotometric Method for the Determination

Data on the Absorbance of the Standard Solutions 1st Meeting

Vol. of Stock (104 mg/L) Absorbance
104 mg dissolved in 100 Conc., mg/L Read 1 Read 2 Read 3
mL dH2O
0 0.0010 0.0000 0.0000
1 0.0050 0.0020 0.0100
2 0.0090 0.0080 0.0050
4 0.0190 0.0170 0.0160
6 0.0330 0.0320 0.0380
8 0.0450 0.0460 0.0460
10 0.0620 0.0620 0.0620

Data on the Absorbance of the Standard Solutions 2nd Meeting

Vol. of Stock (104 mg/L)
104 mg dissolved in 100 Conc., mg/L Absorbance
mL dH2O
0 -0.004 -0.0020 -0.0020
1 0.026 0.0200 0.0220
2 0.046 0.0500 0.0440
4 0.082 0.0850 0.0830
6 0.131 0.1350 0.1370
8 0.171 0.1760 0.1720
10 0.21 0.2090 0.2080
hod for the Determination of Benzoic Acid in Soft Drinks
0.0700

0.0600
Conc., Net Linear Analysis f(x) = 0.00616
0.0500 R² = 0.984264
mg/L Absorbance Parameters Values

Absorbance
0 0.0003 slope 0.0061678 0.0400

1 0.0057 y-int -0.002648 0.0300

2 0.0073 r^2 0.9842647 0.0200
4 0.0173
0.0100
6 0.0343
8 0.0457 0.0000
0 2
10 0.0620
1st meeting

Conc., net Linear Analysis

mg/L Absorbance Parameters Values 0.2500
0 -0.0030 slope 0.0211894
1 0.0240 y-int 0.0008754 0.2000 f(x) = 0.0211894198x
R² = 0.9977986442
2 0.0450 r^2 0.9977986
0.1500

Absorbance
4 0.0825
6 0.1340 0.1000
8 0.1715
0.0500
10 0.2090
0.0000
0 2
-0.0500

2nd meeting
 abs
0.0040
0.0700 Predicted Residual
0.0030
0.0600 -0.002648 0.0030
f(x) = 0.0061678043x - 0.0026478953 0.0020
0.0035199 0.0021
0.0500 R² = 0.9842646528 0.0010
0.0096877 -0.0024
0.0400 0.0220233 -0.0047 0.0000

Residual
0 2
0.0343589 0.0000 -0.0010
0.0300
0.0466945 -0.0010 -0.0020
0.0200
0.0590301 0.0030 -0.0030
0.0100 -0.0040
0.0000 -0.0050
0 2 4 6 8 10 12
-0.0060
Concentration, mg/L
1st meeting

0.2500 abs
0.0008754 -0.0039 0.0080
0.2000 f(x) = 0.0211894198x + 0.0008754266 0.0220648 0.0019
R² = 0.9977986442 0.0060
0.0432543 0.0017
0.1500
0.0856331 -0.0031 0.0040
0.1000 0.1280119 0.0060
0.0020

Residual
0.1703908 0.0011
0.0500
0.2127696 -0.0038 0.0000
0 2
0.0000
0 2 4 6 8 10 12 -0.0020
0.0500
-0.0040
2nd meeting Concentration, mg/L
-0.0060
 0.0040
use linest to compare linear reg f test
0.0030
0.0020
0.0010
0.0000
Residual

0 2 4 6 8 10 12
-0.0010
-0.0020
-0.0030
-0.0040
-0.0050
-0.0060
Concentration, mg/L

0.0080

0.0060

0.0040

0.0020
Residual

0.0000
0 2 4 6 8 10 12
-0.0020

-0.0040

-0.0060
Concentration, mg/L
near reg f test
 Method Validation of the UV Spectrophotometric Method for the Determination of Benzoic A

Data on the UV Absorbance of the Test Sample w/ spike (8ppm) 2nd meeting

Absorbance
Replicate %R
DF= 50 concentration,ppm sd 0.1763523617 109.2286
1 0.201 9.4511378849 mean 9.630745203 109.2286
2 0.201 9.4511378849 G rep 6 1.6383875382 110.9856
0 0.204 9.5917001339 gcrit N=6 1.82 112.157
4 0.206 9.6854082999 since Gcrit>Gmax ,retain 112.157
5 0.206 9.6854082999 115.0853
6 0.211 9.9196787149 111.4737
mean 9.630745203

Data on the UV Absorbance of the Spiked Test Sample w/ o spike 2nd meeting
Absorbance sd 0.0023452079
Replicate
DF=50 cocentration mean 0.0145
1 0.012 0.5957161981 grubbs' test ( 1.4924050145
2 0.012 0.5957161981 gcrit N=6 1.82
3 0.014 0.6894243641 since Gcrit>Gmax ,retain
4 0.015 0.7362784471 grubbs' test ( 1.0660035818
5 0.016 0.7831325301 gcrit N=6 1.82
6 0.018 0.8768406961 since Gcrit>Gmax ,retain
mean 0.7128514056
Determination of Benzoic Acid in Soft Drinks

ing
Linear Analysis of Benzoic
Acid Calibration Curve w/
HCl
Parameters Values
slope 0.021342857
y-int -0.0007142857
r^2 0.9982539782 % benzoic acid in the solution

%R
111.4736724676

ting
 Method Validation of the UV Spectrophotometric Method for the Determination of Benzoic A

Data on the UV Absorbance of the Test Sample (1st Meeting)

Absorbance
Replicate
DF=50
concenration
1 0.031 sd 0.009395 44.0027894
2 0.047 mean 0.0476667 67.43375174
3 0.048 68.89818689
4 0.05 grubbs' test (0.06) 1.3127502 71.82705718
5 0.05 gcrit N=6 1.82 71.82705718
6 0.06 since Gcrit>Gmax ,retain 86.47140865
grubbs' test (0.03) 1.7739868
gcrit N=6 1.82
since Gcrit>Gmax ,retain

Data on the UV Absorbance of the Test Sample w/ HCl (2nd Meeting)

Absorbance
Replicate cocn
DF =50
1 0.021 sd 0.0055648 50.87014726
2 0.023 mean 0.0278333 55.55555556
3 0.026 62.58366801
4 0.03 grubbs' test (0.036) 1.4675659 71.95448461
5 0.031 gcrit N=6 1.82 74.29718876
6 0.036 since Gcrit>Gmax ,retain 86.0107095
grubbs' test (0.021) 1.2279633
gcrit N=6 1.82
since Gcrit>Gmax ,retain
for the Determination of Benzoic Acid in Soft Drinks

eting)
Linear Analysis of Benzoic Acid Calibration Curve w/ HCl

Parameters Values
slope 0.0341429
y-int 0.0009524
r^2 0.9981419

day 1 day 2
44.002789 50.870147
67.433752 55.555556
68.898187 62.583668
71.827057 71.954485
Meeting) 71.827057 74.297189
Linear Analysis of 86.471409 86.01071
Benzoic Acid
Calibration Curve w/ 2 tailed t test at 95% confidence
ParametersHCl Values
slope 0.0213429
y-int -0.000714
r^2 0.998254
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Column 1 6 410.4603 68.41004 189.2941
Column 2 6 401.2718 66.87863 169.9532

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 7.035707 1 7.035707 0.039169 0.847079 4.964603
Within Groups 1796.236 10 179.6236

Total 1803.272 11