Abstracts / International Journal of Infectious Diseases 73S (2018) 3–398
Final Abstract Number: SADI 03.004
Session: Microbiología y Antibióticos/Microbiology and Antibiotics
Date: Thursday, March 1, 2018
Time: 09:00-10:30
Room: Libertador C
Type: Oral Presentation
Stress survival and antimicrobial
characterization of S. Enteritidis from different
hosts in Chile
M. Fresno ∗ , P. Retamal
Universidad de Chile, Santiago, Chile
Background: Salmonella is a Gram negative bacteria that colo-
nizes the intestinal tract of humans and animals. Infections with
S. Enteritca are of great concern to the public health, being one of
the main causes of foodborne diseases in Chile and the world. Wild
birds are of increasing importance to public health, because of their
association with highly transmisible zoonotic pathogens, and their
hability to disseminate them. In this work, the S. Enteritis strains
isolated from different hosts were charactherized to enlighten the
human-animal interfase of this bacteria in Chile. Phenotypic assays
were done to determine the pathogenic potential of the isolates of
Salmonella of different hosts.
Methods & Materials: In this work we determined the oxidative
stress survival (H2 O2 15 mM and NaNO2 10 mM), acidic pH (pH3),
starvation (D10 and D20) and antimicrobial susceptibility of 90 S.
Enteritidis strains, isolated from poultry, shore birds and humans
from different regions of the country, comparing between each
strain and among hosts. The statistical analysis were done with
InfostatÒ software.
Results: The results show that there is statistical differences
among the studied strains in all stress survival assays. When
analyzed by host, significant differences (p < 0.05) were found in
starvation assays (D10 and D20). No differences were found in the
oxidative stress and acidic pH assays when grouped by host. When
analyzing antimicrobial susceptibility, most of the strains have
resistance to at least one antimicrobial. The strains isolated from
shore birds had a high resistance to tetracycline (73,3%), human
strains had a higher resistance to sulfisoxazol (40%) and in poultry
the highest resistance was for nalidixic acid (43,3%). We detected
multiresistance in the 36,7% of the strains, most of which came
from shore birds (42,4%). Finally, grouped by host, there are no
differences (p > 0.05) in the presentation of the resistance.
Conclusion: Shore birds can be reservoirs of Salmonella strains
with potential risk to public and animal health, and could partially
explain the progressive rise in the incidence of these serotype-
associated outbreaks.
There is a high resistance rate of AM commonly use in Chile,
which justifies monitoring these hosts as bio-indicators of AM resis-
tance spread in the environment.
https://doi.org/10.1016/j.ijid.2018.04.3444
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7
Final Abstract Number: SADI 03.005
Session: Microbiología y Antibióticos / Microbiology and Antibiotics
Date: Thursday, March 1, 2018
Time: 09:00-10:30
Room: Libertador C
Type: Oral Presentation
Community-acquired uncomplicated urinary
tract infections (UTI): current etiology and
antimicrobial susceptibility in Argentina. A
prospective, observational, multicentre study
M.J. Lopez Furst 1,∗ , A. Mykietiuk 2 , P. Pessacq 3 ,
P.G. Scapellato 4 , L. Clara 5 , C. Nemirovsky 5 ,
A. Otreras 6 , J. Martinez 7 , M. Gañete 8 , G. Bertoni 9 ,
A. Sandor 10 , M. Galvez 11 , A. Crespo 12 ,
M. Peralta 13 , F. Barberis 14
1 Sanatorio Julio Mèndez, Buenoa Aires, Argentina
2 Instituto Medico platense, La Plata, Argentina
3 Hospital Italiano de La Plata, La Plata, BA, Argentina
4 Hospital D F Santojanni, Buenos Aires, Argentina
5 Hospital Italiano, Buenos Aires, Argentina
6 Hospital Santojanni, Buenos Aires, Argentina
7 Hospital Britanico de Buenos Aires, Buenos Aires,
Argentina
8 Clinica Juncal, Temperley, Argentina
9 Hospital Rodolfo Rossi, La Plata, Argentina
10 Instituto Sagrado Corazon, Buenos Aires, Argentina
11 Hospital Castro Rendon, Neuquen, Argentina
12 Hospital Iturraspe, Santa fe, Argentina
13 AMEBPBA, Buenos Aires, Argentina
14 Instituto Dupuytren, Buenos Aires, Argentina
Background: UTI are a frequent problem in primary care and
represent one of the main reasons for the prescription of antibi-
otics. Latest IDSA guidelines recommend local prospective studies
and unbiased resistance surveillance of uropathogens in order to
improve antimicrobial prescription and prevention of resistance.
This study aims to analyze the etiology and antimicrobial suscep-
tibility of uropathogens causing uncomplicated UTI from clinical
practice to laboratory data in order to avoid bias in resistant pat-
terns.
Methods & Materials: Methods: prospective, multicenter (14
hospitals), observational study (08/16/16 to 08/31/17). Inclusion
criteria: non-pregnant woman older than 15 years with urinary
symptoms and positive urine culture. Microbiological procedures
were performed according to the CLSI standards.
Results: Out of 487 patients recruited, 467 fulfilled the inclu-
sion criteria. Median age 37.6 years, 339 (72%) of episodes were
cystitis. Isolates: E. coli 82%, Proteus spp. 4%, Klebsiella spp. 3%.
Global antibiotic resistance: ampicillin-sulbactam 22%, trimetho-
prim /sulfamethoxazole 26.7%, 1st generation cephalosporins 9.9%,
quinolones 9%, nitrofurantoin 6.6%. Analyzing in particular E. coli
resistance: 22.4% for ampicillin-sulbactam, 28% for trimetho-
prim/sulfamethoxazole, 1st generation cephalosporins 8.7%, for
ciprofloxacin, 9.6% and for nitrofurantoin 0.6%. There were no dif-
ferences in resistance when comparing the group of patients over
50 years of age (397) versus those under 50 (97). We also observed
that previous antibiotic use in patients younger than 50 years old
(52), was associated with a significant increase in the resistance
rates, ampicillin-sulbactam 24%(NS), trimethoprim /sulfamethox-
azole 41.3%(p = 0.020; OR: 2.07), 1st generation cephalosporins
21.7%(p = 0.001; OR 3.56) quinolones 34.1%, p < 0.001; OR 10.77),
nitrofurantoin 8% (NS).