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Liquid Chromatograph
SCOPE OF WORK
1. SCOPE
This document provides details of the procedures necessary for the successful completion of a Preventive
Maintenance (PM) service call for the 1100 Series Liquid Chromatograph. It clarifies responsibilities
and assures uniform delivery of the service. Only Support Providers trained and certified by Agilent
Technologies will provide the service. Support Providers are Agilent Customer Engineers (CE’s)
and/or Agilent Authorized Support Providers (ASPs). References to other documents are included to
supply additional information (such as part numbers) or to provide clarity.
Delivery time for completion of the PM call is approximately 4.0 hours for a system comprising a pump,
degasser, column compartment, autosampler or manual injector, and a detector. One hour should be
added for each additional detector. Time starts when the Support Provider arrives at the Customer site
and ends when he/she leaves the site. The PM Checklist (a separate document) details the steps
necessary for completion.
NOTE: This document is to be used by Agilent Employees and Agilent ASPs, and is not to be
provided to Customers.
2. CUSTOMER RESPONSIBILITIES
In preparation for the service call, the Call Initiator (see section 3.1 below for a definition of the Call
Initiator) verifies with the Customer that the Support Provider has:
• Access to the liquid chromatograph scheduled for PM and adequate downtime to complete the
procedure.
• Adequate working space around the liquid chromatograph, with all supplies nearby.
• A representative (for example, the operator) present at all times when Support Provider is
performing this service.
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Agilent 1100 Series
Liquid Chromatograph
• The system flushed with HPLC grade water, samples removed from the Autosampler (ALS), and
analytical columns removed from the column compartment.
• Normal operating supplies available including consumables and usage-dependent items such as
paper, pens, and solvents (HPLC grade water, isopropanol, methanol, acetone).
• Failure to provide the above may reduce applicable tests performed in the PM (that is, pressure test,
test injection).
3. AGILENT RESPONSIBILITIES
3.1 Scheduling—The Call Initiator
The service call begins with a call to the Customer by the “Call Initiator.” The Call Initiator may be the
Support Provider, a workload administrator, or a dispatcher, depending on local processes. The Call
Initiator:
1. Calls the Customer, schedules the date and time for service, and confirms Customer preparation.
2. Discusses possible problem areas before the Support Provider goes on site.
3. Asks the Customer to log a service call, if the instrument is not functional.
3.2.1 Delivery
1. Brings all necessary tools, supplies and parts to complete the procedure (see 1100 Series
Service/Reference Manuals for list of tools, supplies and parts) including:
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3. Supplies consumables used during the PM. See sections 4.1.4 through 4.1.11 and 4.1.16 of this
document for a list of included consumables.
3.2.2 Documentation
3. Completes the Firmware Update/Installation Record Form if 1100 or 1200 firmware was updated.
4. Asks the Customer to sign the PM Checklist acknowledging the completion of service.
6. Affixes all data generated to the PM Checklist and gives it to the Customer for addition to the
instrument logbook.
4. PM CHECKLIST
Refer to the 1100 Series PM Checklist.
Support Provider completes the Checklist whether or not the instrument is fully operational.
1. Customer has system flushed with HPLC grade water, all samples removed from the ALS, and
analytical columns removed from the column compartment.
2. Customer has isopropanol (IPA) and HPLC grade water in the appropriate mobile phase containers
available for the procedure.
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4.1.2 PM checklist
1. Verify that the Customer has removed analytical column. If still present, remove it now.
2. Verify with the Customer that system was flushed with HPLC grade water. If not, flush system
now.
3. If 1100 firmware is to be updated, verify that Customer has backed-up all methods.
5. If any module fails a test, record the results in the Test Results box and complete the rest of the
PM Checklist.
NOTE: In the event of any test failure, the Support Provider will log a service call, if the
Customer has a service contract. If the Customer does not have a service contract, the
Support Provider will inform the Customer of the failure (leak, inoperable sensor, test,
etc.), and offer to log a Time and Material service call for the Customer.
− Follow the instructions provided by the Firmware Update/Installation Record Form for
1100/1200 Series HPLC Instruments available from the EPI Warehouse or Compliance CD-
ROM to check the firmware revisions currently installed.
− Review the compatibility text within the firmware update package to ensure the impact of
performing the update is fully understood by you and the customer prior to updating.
− If firmware is not current, ask Customer if they would like the firmware updated. If the
Customer chooses to have the firmware updated, confirm with the customer that they require
this form for their records and follow the procedure in the Firmware Update/Installation Record
Form . This form is to be used only once.
− If the firmware is updated again at a later date, an additional form should be used after obtaining
the required Customer approval.
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2. Record the module model number and serial number on the PM Checklist. If the model/serial
number is not included in the 1100 system, record N/A (not applicable) in this area and N/A in the
module Tests Result box.
3. Check the instrument logbook for recently serviced items. Exclude maintenance on items that were
replaced within 90 days of the current PM.
4. Perform a general inspection of each LC module. Alert the Customer to any potential safety issues
(waste container full, frayed wiring, drain tubes blocked, etc.).
5. Check that all waste trays/interfaces are properly mounted as per the 1100 Series Installation Guide.
6. Check for proper mounting and functionality of all leak sensors. Wash off each sensor with water.
If the sensor is functioning, the LC module indicator light will change to red indicating a leak. Dry
off sensor using a tissue or paper towel.
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Agilent 1100 Series
Liquid Chromatograph
1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
3. Remove and disassemble the pump head following the procedure in the 1100 Pump
Service/Reference Manual.
4. Clean the plungers with methanol or toothpaste. If toothpaste is used, take care to wash off any
residual toothpaste with water or methanol.
5. Clean the support rings and springs in the pump housing with water or methanol. A toothbrush can
be used to help remove stubborn deposits.
6. Exchange the pump seals following the procedure in the 1100 Pump Service/Reference Manual.
7. If the seal wash option is installed remove and replace wash seal and gasket as specified in
1100 Pump Service/Reference Manual.
8. Reassemble the pump head assembly following the procedure in the 1100 Pump Service/Reference
Manual.
9. Prepare a bottle of isopropanol and perform the seal wear-in procedure as defined in the 1100 Pump
Service/Reference Manual. The use of a restriction capillary (5022-2159) is required.
NOTE: This procedure is required for standard seals only (5063-6589). Performing this
procedure on the normal phase application seals (0905-1420) will damage them.
10. Replace the purge valve frit, gold seal, and plastic cap following the procedure in the 1100 Pump
Service/Reference Manual.
11. Replace the outlet ball valve sieve (binary pump), gold seal, and plastic cap as described in the 1100
Pump Service/Reference Manual.
12. Using the same bottle of isopropanol, immerse solvent filter into the solvent and attach restriction
capillary to purge valve.
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13. Prime appropriate channel at 3 mL/min (lower flow rate may be needed to avoid overpressure) for
10 minutes with the purge closed. For a binary pump, purge Channel A. For a binary pump with a
solvent selection valve, purge Channel A2. For a quaternary pump, purge Channel D. This will
require >30 mL of isopropanol.
14. While purging, begin maintenance on the Sampler or Manual Injector and Column Compartment, if
installed.
16. Cap the outlet of the pump with a blanking nut (01080-83202). Perform the built-in leak test
following the procedure in the 1100 Pump Service/Reference Manual.
17. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled pump or a loose fitting.
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1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
3. Remove and disassemble the pump head following the procedure in the 1100 Prep Pump
Service/Reference Manual.
4. Clean the plungers with methanol or toothpaste. If toothpaste is used, take care to wash off any
residual toothpaste with water or methanol.
5. Exchange the pump seals (front and rear, left and right) following the procedure in the 1100 Prep
Pump Service/Reference Manual.
6. Reassemble the pump head assembly following the procedure in the 1100 Prep Pump
Service/Reference Manual.
7. Replace the filter cup in the multi assembly following the procedure in the 1100 Prep Pump
Service/Reference Manual.
8. Pump 200 mL of IPA through the pump before replacing the SST filter assembly.
9. Replace the SST filter assembly following the procedure in the 1100 Prep Pump Service/Reference
Manual.
10. Replace the seal wash pump cartridge following the procedure in the 1100 Prep Pump
Service/Reference Manual.
11. Perform the EMPV cleaning procedure as described in the 1100 Prep Pump Service/Reference
Manual.
12. Perform the built-in leak test following the procedure in the 1100 Prep Pump Service/Reference
Manual.
13. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled pump or a loose fitting.
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1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
3. Remove and disassemble the pump head following the procedure in the 1100 Capillary or Nano
Pump Service/Reference Manual.
4. Clean the plungers with methanol or toothpaste. If toothpaste is used, take care to wash off any
residual toothpaste with water or methanol.
5. Exchange the pump seals following the procedure in the 1100 Capillary or Nano Pump
Service/Reference Manual.
6. Reassemble the pump head assembly following the procedure in the 1100 Capillary or Nano Pump
Service/Reference Manual.
7. Replace the purge valve frit, gold seal, and plastic cap following the procedure in the 1100 Capillary
or Nano Pump Service/Reference Manual (that is if the pump is used in “Normal Mode” and a
manual purge valve is installed).
8. Replace the outlet ball valve sieve, gold seal, and plastic cap as described in the 1100 Capillary or
Nano Pump Service/Reference Manual.
10. Perform the EMPV cleaning procedure following the instructions in Service Notes G1376-04A and
G1376-05.
11. Prepare a bottle of isopropanol and perform the seal wear-in procedure as defined in the
1100 Capillary or Nano Pump Service/Reference Manual. The use of a restriction capillary (5022-
2159) is required.
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NOTE: This procedure is required for standard seals only (5063-6589). Performing this
procedure on the normal phase application seals (0905-1420) will damage them.
12. Using the same bottle of isopropanol, immerse solvent filter into the solvent and prime Channel A2
at 2500 μL/min for 10 minutes. This will require >25 mL of isopropanol.
13. Cap the outlet of the pump with a blanking nut (01080-83202).
14. Perform the built-in pressure test following the procedure in the 1100 Capillary or Nano Pump
Service/Reference Manual.
− If you use the system in micro mode, perform the “Micro mode pressure test.”
− If you use the system in normal mode, perform the “Normal mode pressure test.”
15. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled pump or a loose fitting.
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1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
2. Change the needle and needle seat using the change needle function of the Control Module or
ChemStation maintenance functions. Follow the procedure in the 1100 Autosampler
Service/Reference Manual.
4. Replace the rotor seal following the procedure in the 1100 Autosampler Service/Reference Manual.
5. Check that the chiller unit drain is positioned correctly (if applicable).
6. Purge ALS flow path with isopropanol for 5 minutes using the same channel as the previous
applicable pump maintenance section (4.1.4, 4.1.5, or 4.1.6).
7. If the column compartment contains a column switching valve, cap the outlet of the ALS with a
blanking nut. Otherwise, cap the outlet of the column compartment with the blanking nut (01080-
83202).
8. Run the built-in pressure test following the procedure in the 1100 Pump Service/Reference Manual.
9. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled rotor seal or a loose fitting.
4.1.8 G1367A/G1377A micro well plate sampler and G2258A dual loop autosampler
1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
3. Change the needle and needle seat using the change needle function of the Control Module or
ChemStation maintenance functions. Follow the procedure in the 1100 Well Plate/Autosampler
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Service/Reference Manual. For the Dual Loop ALS G2258A, this procedure is performed only if
the needle is deformed or needle/seat is leaking (must be billed separately as a repair).
NOTE: The G2258A DLAs buffer loop capillary becomes shorter every time a needle is replaced.
After two to three replacements of the needle the buffer loop capillary must also be
exchanged (must be billed separately as a repair) when it has become too short to connect
the needle to it.
4. Inspect all G2258A DLA tubings and capillaries for visual signs of damage (kinks) and replace if
necessary (must be billed separately as a repair).
5. Replace the rotor seal following the procedure in the 1100 Series Well Plate Sampler & Micro Well
Plate Sampler Service/Reference Manual.
6. Replace the peristaltic pump cartridge following the procedure in the 1100 Series Well Plate
Sampler & Micro Well Plate Sampler Service/Reference Manual.
7. Check that the chiller unit drain is positioned correctly (if applicable).
8. Purge ALS flow path with isopropanol for 5 minutes using the same channel used in section 4.1.4.
9. If the column compartment contains a column switching valve, cap the outlet of the ALS with a
blanking nut. Otherwise, cap the outlet of the column compartment with the blanking nut (01080-
83202).
10. Run the built-in pressure test following the procedure in the 1100 Pump Service/Reference Manual.
11. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled rotor seal or a loose fitting.
1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
2. Replace the rotor seal following the procedure in the 1100 Series Manual Injector Service/Reference
Manual.
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Liquid Chromatograph
3. Purge injector flow path with isopropanol for 5 minutes using the same channel as the previous
applicable pump maintenance section (4.1.4, 4.1.5, or 4.1.6).
4. If the column compartment contains a column switching valve, cap the outlet of the injector
(port 3) with a blanking nut. Otherwise, cap the outlet of the column compartment with the
blanking nut (01080-83202).
5. Run the built-in pressure test following the procedure in the 1100 Pump Service/Reference Manual.
6. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled rotor seal or a loose fitting.
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1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
2. If a column switching valve is installed, replace the rotor seal as specified in the 1100 Column
Compartment Service/Reference Manual.
3. Purge column compartment with isopropanol for 5 minutes using the same channel used in
section 4.1.4.
4. Cap the outlet of the column compartment with a blanking nut (01080-83202).
5. Run the built-in pressure test following the procedure in the Pump Service/Reference Manual.
6. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled rotor seal or a loose fitting.
7. Perform the thermostat test following the procedure in the ChemStation’s Diagnosis View and
attach results to the PM Checklist. If there is no ChemStation installed, create your own method on
the Control Module:
− Both Peltier elements must initially be equilibrated to 25 °C. This temperature is held for
12 seconds, and then the set point is changed to 20 °C. The time required to reach 20 °C is a
measure of the cooling efficiency of the Peltier elements.
− At 3.5 minutes, the set point is changed to 30 °C, and both elements begin heating. The time
required to reach 30 °C is a measure of heating efficiency.
− Upon completion of the test, the cooling and heating efficiencies of both Peltier elements must
be calculated and compared to the acceptance criteria: the cooling rate for each Peltier must be
greater than 2 oC/min, the heating rate for each Peltier must be greater than 3 oC/min.
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• Record the time t1, when the set point for cooling (20 oC) is reached, subtract
t0= 0.21 min. The cooling efficiency is = 5 oC / (t1 –t0)
• Record the time t3, when the set point for heating (30 oC) is reached, subtract
t2= 3.51 min. The heating efficiency is = 10 oC / (t3 –t2)
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Agilent 1100 Series
Liquid Chromatograph
1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
2. Replace the rotor seal (and stator face) as specified in the 1100 Series Valves Operator’s Manual.
3. Purge valve(s) with isopropanol for 5 minutes using the same channel used in section 4.1.4.
5. Run the built-in pressure test following the procedure in the pump Service/Reference Manual.
6. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled rotor seal or a loose fitting.
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Agilent 1100 Series
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1. Verify that the outlet of the flow cell is unrestricted and flows directly to the waste reservoir.
2. Remove the flow cell from the detector and establish 2 mL/min flow rate using HPLC grade water.
5. Perform the holmium oxide test as detailed in the 1100 VWD Service/Reference Manual Guide.
Record the value in the Test Result box if this test passes.
− If this test fails, perform the wavelength calibration test also found in the 1100 VWD
Service/Reference Manual Guide.
− If the wavelength calibration test is successful rerun the holmium oxide test. Record result in
the Test Result box.
6. Perform the intensity test as specified in the 1100 VWD Service/Reference Manual and attach
results to the PM Checklist.
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4.1.13 G1315A/B/C Diode Array Detectors and G1365A/B/C Multiple Wavelength Detectors
1. Verify that the outlet of the flow cell is unrestricted and flows directly to the waste reservoir.
2. Remove the flow cell from the detector and establish 2 mL/min flow rate using HPLC grade water.
5. Perform the filter test as specified in the 1100 DAD/MWD Service/Reference Manual and attach
results to the PM Checklist.
6. Perform the dark current test as specified in the 1100 DAD/MWD Service/Reference Manual and
attach results to the PM Checklist.
7. Perform the holmium oxide test as detailed in the 1100 DAD/MWD Service/Reference Manual
Guide. Record if this test passes.
− If this test fails, perform the wavelength calibration test also found in the 1100 DAD/MWD
Reference Guide.
− If the wavelength calibration test is successful rerun the holmium oxide test. Record result in
the Test Result box.
8. Perform the intensity test as specified in the 1100 DAD/MWD Service/Reference Manual and attach
results to the PM Checklist.
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1. Verify that the outlet of the flow cell is unrestricted and flows directly to the waste reservoir.
2. Remove the flow cell from the detector and establish 2mL/min flow rate using HPLC grade water.
Note: If lamp intensity appears low, check the window on the lamp housing. Clean or replace the
quartz window.
5. Perform the wavelength verification test using the Raman band as specified in the 1100 FLD
Service/Reference Manual and attach results to PM Checklist. If this test fails, perform the
wavelength calibration test as specified in the 1100 FLD Service/Reference Manual.
1. If not already performed, flush flow cell with water for 10 minutes.
NOTE: The instrument has to be switched to "Purge" for the flushing of both the reference and
the sample side of the flow cell.
2. Check the diode balance as specified in the 1100 RID Service/Reference Manual. Verify that the
value does not fall outside the range of –0.2 to +0.2. If value is outside the specified range, perform
the optical balance procedure as specified in the 1100 RID Service/Reference Manual. Verify that
the diode balance is –0.2 to +0.2 and record this value in the Test Result box of the PM Checklist.
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1. This maintenance procedure requires the consumables found in 1. in the parts list at the end of
this document.
2. Replace the inlet/waste tubing assembly (G1364A/B/C) or the capillary assembly (G1364D) as
specified in the 1100 series Fraction Collectors Service/Reference Manual.
3. Replace the valve to the needle tubing assembly (not for G1364D) as specified in the 1100 Series
Fraction Collectors Service/Reference Manual.
4. Check that the chiller unit drain is positioned correctly (if applicable).
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1. If ChemStation is the instrument controller, attach all printouts from tests performed to the
PM Checklist.
2. If the instrument firmware was updated at the request of the Customer, complete the IQ Attachment
Protocol and append it to the instrument IQ.
3. Make a test injection of a dilute mixture of acetone in water with no column. One drop in a
2 mL vial of water should be sufficient. Use λ 265 nm, 4 nm BW, >0.01 min PW. Run time should
be 2 minutes or less. The presence of a peak will indicate that the system is plumbed correctly. IF
THE PM IS BEING PERFORMED IN ADVANCE OF AN OQ/PV, USE THE PROTOCOL AS A
GUIDE FOR THE CHECKOUT.
NOTE: Many Customers prefer that water be left on the system. Confer with the Customer prior
to loading Customer’s mobile phase.
NOTE: Some Customers do not approve of the placement of a PM sticker on the instrument.
This is optional and only performed if the Customer approves.
7. Customer and Support Provider sign the PM Checklist indicating service was performed.
5. ACKNOWLEDGEMENTS
Tefzel® is a registered trademark of E.I. DuPont de Nemours Co., Inc.
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Agilent 1100 Series
Liquid Chromatograph
6. PARTS REQUIREMENTS
Please Note: Any parts, that are not included in the parts lists given below, are not part of a
regular Preventive Maintenance Procedure, they are not included in the price of this Service.
If a system has a special setup/configuration that requires the use of additional or special parts for the
instrument service, then these parts must be ordered separately and billed as a repair.
If a customer requests additional service that is not listed in the procedures described above to be
performed at his/her instrumentation, then the additional service must be ordered separately and billed
as a repair. Additionally required parts must be ordered and billed separately.
G1310A/11A/12A – Pump
This maintenance procedure requires the following consumables:
Purge-Valve Assembly
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Purge-Valve Assembly
Seal wash items are needed only, if the seal wash option is installed.
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This maintenance procedure requires the following consumables for each pump (the parts are all
included in the G1361-68710 Prep. Pump PM kit):
Multi-Assy
1x Cartridge 5042-8507
1x Tubing 0890-1764
2x Fittings 5042-6422
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Filter Assembly
1x Frit 5022-2185
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For G1313A/29A (these parts are all included in the G1313-68730 Autosampler PM kit):
For G1387A
1x Needle G1329-80001
For G2260A
1x Needle G2260-87201
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G1367A Well Plate Sampler, G1377A Micro Well Plate Sampler and G2258A Dual
Loop Autosampler
For G1367A (these parts are all included in the G1367-68730 Wellplate Autosampler PM kit):
1x Needle G1367-87201
For G1377A
1x Needle G1377-87201
For G2258A
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Note: If the following parts replacements are necessary because the needle is deformed or if
needle/seat are leaking these are not covered by the PM and they MUST be BILLED
SEPARATELY as a REPAIR:
1x Needle G2258-87306
Note: After a needle has been replaced several times, the buffer loop capillary will become too
short and will also have to be replaced:
G1328A/B – Manual Injection Valve and 5065-9922 Prep. Manual Injection Valve
1x Rotor seal 0101-0623 (Vespel®) for analytical scale manual injection valve (G1328A/B) or
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This maintenance procedure requires the following consumables only if a column-switching valve is
installed (G1316A #055 1100 Series 2PS/6PT or G1316A #057 1100 Series 2PS/10PT Valve options):
1x Rebuild Kit 0101-1359 (Rotor Seal and Stator Face) for valve option #057.
1x Rebuild kit 0101-1359 (Rotor seal and Stator Face) for G1157A
1x Rebuild kit 0101-1358 (Rotor seal and Stator Face) for G1158A
1x Rebuild kit 0101-1290 (Rotor seal and Stator Face) for G1159A
1x Rebuild kit 0101-1288 (Rotor seal and Stator Face) for G1160A
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The maintenance procedure requires NO consumables except the G1321A which may require the
following part:-
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