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Natural sources of organic matter includes plant decay and dead leaf. However, plant
growth and decay maybe unnaturally accelerated when nutrients and sunlight are overly
abundant due to human influence. Microorganisms such as bacteria or protozoa are
responsible for decomposing organic waste. When organic matter such as dead plant, leaves,
grass clipping, manure, sewage or even food waste is present in a water supply, the bacteria
will begin process of breaking down this waste. When this happen, much of the available
dissolved oxygen is consumed by aerobic bacteria, robbing other aquatic organisms of the
oxygen they need to survive.
OBJECTIVES
PROBLEM STATEMENT
DISTILLED WATER
BOD INCUBATOR
PROCEDURE
1. 5ml of water sample A and B was poured into two of BOD bottle with label A and B
which was to differentiated between them.
2. The two of sample BOD bottle was filled with dilution water. Added the dilution
water until the bottles were completed fully.
3. Carefully cap of BOD bottles with the ground-glass stopper. Tip the bottle to one side
and checked whether it have an air bubble.
4. If an air bubble was present, added glass beads to the bottle until the bubble was
removed. cap the bottle and checked again for an air bubble. Repeat if necessary.
5. If no bubble was present in the bottle sample, create a water seal by adding distilled to
the top of the BOD bottle to minimize evaporation from the water seal.
6. The initial DO concentration of each bottle sample (A1) was measured by using DO
meter before placed in to the incubator.
7. Carefully inserted the self-stirring sensor into the BOD bottle, avoiding air
entrapment.
8. Turn on the stirrer and allow for a few minutes until the DO and temperature reading
was stabilized.
9. Placed the two bottles in the incubator at 22.8 °C and incubated for 5 days.
10. At the end of 5 days, remove the BOD bottles from the incubator and measure the
final DO by using DO meter.
RESULT AND DATA
To determine the value of the BOD in mg/L, use the following formula:
For example:
Sample size = 50 mL