Bioactive Compounds from Penicillium oxalicum WR3,

A Marine Derived Fungus from the Sponge Haliclona fascigera

Dian Handayani1*, Netty Suharti1, Retno Amilia1, Triana Hertiani2

1
Faculty of Pharmacy/Sumatran Biota Laboratory, Andalas University, Kampus Limau
Manis, Padang 25163, West Sumatra, Indonesia
2
Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, Yogyakarta, Indonesia

*corresponding:
Name: Dian Handayani
Address: 1Faculty of Pharmacy/Sumatran Biota Laboratory, Andalas University, Kampus
Limau Manis, Padang 25163, West Sumatra, Indonesia
Email: dianhandayani@phar.unand.ac.id

ABSTRACT

Marine derived fungi associated with marine sponges are known produced bioactive
secondary metabolite. As part of our continuous efforts in search for cytotoxic and
antibacterial compounds from natural sources, a study on the bioactive compound of the
fungus Penicillium oxalicum (WR3) isolated from the marine sponge Haliclona
fascigera has been conducted. Two known fungal metabolite, culvularin (1) and
sydowinin B (2) were isolated from the culture media of this fungus. Both compounds
were evaluated for the antibacterial and cytotoxic activities against Hela and T47D cell
lines. The Minimum Inhibitor Concentrations (MIC) of culvularin (1) and sydowinin B
(2) againts against Staphylococcus aureus were 125 µg/disc and 500 µg/disc,
respectively. Both compounds were shown to be cytotoxic against the human cancer cell
line, T47D and HELA in vitro (IC50 = 142.66 ppm and 164.88 ppm).

Keywords: Penicillium oxalicum, Halicona fascigera, Antibacterial activity, Cytotoxic

activity, culvularin, sydowinin B.
ABSTRAK
Senyawa Bioaktif dari Penicillium oxalicum WR3,
Jamur Laut yang Berasal dari Spons Haliclona fascigera

Jamur laut yang berasosisi dengan spons laut dikenal menghasilkan metabolit sekunder
bioaktif. Sebagai bagian dari upaya kami yang berkesinambungan dalam mencari
senyawa sitotoksik dan antibakteri dari sumber daya alami, penelitian tentang senyawa
bioaktif dari jamur Penicillium oxalicum (WR3) yang diisolasi dari spons laut
Haliclona fascigera telah dilakukan. Dua senyawa metabolit jamur yang telah dikenal,
culvularin (1) dan sydowinin B (2) diisolasi dari media kultur jamur ini. Kedua senyawa
dievaluasi untuk aktivitas antibakteri dan sitotoksik terhadap kultur sel Hela dan T47D.
Konsentrasi Inhibitor Minimum (MIC) dari culvularin (1) dan sydowinin B (2) terhadap
Staphylococcus aureus masing-masing adalah 125 μg/disc dan 500 μg/disc. Kedua
senyawa terbukti sitotoksik terhadap kultur sel kanker manusia, T47D dan HELA secara
in vitro (IC50 = 142,66 ppm dan 164,88 ppm).

Kata kunci: Penicillium oxalicum, Halicona fascigera, Aktivitas Antibakteri, Aktivitas

Sitotoksik, Culvularin, Sydowinin B.