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Mas, Mylyn L.
Ong, Dyan S.
PhCh 135-PHC
ASSAY OF LACTIC ACID
Titrate excess alkali with 1N Sulfuric Volume of Back Titrant Used (1.0 N H2SO4); Nf= 1.0
Acid TS Volume Reading Trial 1 Trial 2 Blank
Initial Reading 0.00 mL 0.00 mL 0.00 mL
*Perform a blank determination Final Reading 21.50 mL 21.65 mL 48.00 mL
Total Volume Used 21.50 mL 21.65 mL 48.00 mL
Reasons for Important Steps:
% A.I = [NT X Nf X (Vblank- Vactual)]backtitrant] X MWanalyte
The solution is boiled for 20 minutes to f x 1000
sample wt (g)
speed up the reaction
Trial 1:
The titration is done in room temperature [1.0 N X 1.0 N x (48 mL-21.50mL) x 90.08/1000)]
because many indicators give different 2.7024
indications at different temperatures. =88.33 %
C3H5O3H (aq) + NaOH(aq) C3H5O3Na(aq)+ H2O Skoog, D., West, D., Holler, F., Crouch, S. (2014). Fundamentals of
(lactic acid) (sodium lactate) analytical chemistry (9th ed.). California: Cengage Learning.
Factor: 1 since 1 mole of NaOH is needed to neutralize United States Pharmacopeial Convention, Inc. (2012). United States
1 mole of lactic acid. Pharmacopeia 35th Revision and National Formulary 30th Edition
Neutralization reaction: (p.3630). Maryland: USPCI.
2NaOH (aq) + H2SO4 (aq) Na2SO4 (aq) + H2O