21) Who discovered split gene ?

(A) P. Chambor
(B) Johanssen
(C) P. Leader
(D) P.Sharp and R.Roberts
Correct Answer :P.Sharp and R.Roberts

22) Rate of mutation in nature is

(A) 1x105
(B) 1x108
(C) 1x105
(D) 1x1012
Correct Answer :1x105

23) Artificial synthesis of mRNA was done by ?

(A) M.Nirenberg (1951)
(B) H.G. Khurana
(C) Okha (1955)
(D) F. Griffith
Correct Answer :H.G. Khurana

24)
Role of ‘Single Stranded Binding’ (SSB) protein in DNA replication
is
(A) Separator Primer
(B) Stabilizes Unwounded DNA
(C) Prevents Supercoiling
(D) None of above
Correct Answer :Stabilizes Unwounded DNA

25) Clover leaf model of tRNA was proposed by.

(A) Nirenberg & Mathai
(B) Robert Holley
(C) Okha
(D) H.G.Khurana
Correct Answer :Robert Holley
26)
In DNA replication the Okazaki fragments on the lagging strand a
re joined together by.
(A) Helicase
(B) DNA Ligase
(C) DNA Polymerase
(D) Correct Answer :DNA Ligase

27)
According to Meselson & Stahl experiment of DNA replication, DN
A molecules are
centrifuged in CsCl density gradient, they separate on the basis of
?
(A) Mass Number
(B) Volume
(C) Density
(D) All of above
Correct Answer :All of above

28) In meiosis, chromosomes are reduplicated during

(A) Prophase I
(B) Prophase II
(C) Interphase
(D) Inter Kinesis
Correct Answer :Interphase

29) After a m-
RNA has been translated and leaves the ribosome or polysome, it
is.?
(A) Dehydrated
(B) Degraded
(C) Dehydrolysed
(D) Oxidised
Correct Answer :Degraded
30)
In mitosis, just after duplication, the two sister chromatids remai
n attached at
(A) Spindle
(B) Asters
(C) Centromere
(D) Centrioles

Correct Answer : Centromere

1)
Who among the following rediscovered Mendel’s laws of genetics?
(A) De Vries
(B) Carl Correns
(C) Tschermark
(D) All of the above
Correct Answer : All of the above

2) For genetical units, Mendel used the word _______ and Corren
s called them as _______.
(A) Factors, Elements
(B) Elements, Factors
(C) Gene, Exon
(D) Exon, Gene
Correct Answer : Elements, Factors

3) Altman (1892), referred to the mitochondria as

(A) Bioplast
(B) Power house of cell
(C) Thermoplast
(D) None of above
Correct Answer : Bioplast

4) The genetic code (Triplet) is

(A) Degenerate
(B) Ambiguous
(C) Degenerate and Ambiguous both
(D) None of above
Correct Answer : Degenerate
5) A triplet genetic code was first suggested by.
(A) Johansson
(B) Gamow
(C) Watson
(D) Crick
Correct Answer : Gamow

6) Watson & Crick (1953), explained double helical structure of D

NA with the help of
(A) HPLC
(B) Xray diffraction
(C) Thermocycler
(D) Southern Blotting
Correct Answer : Xray diffraction

7)
In a double helical DNA molecule the two helices have a distance
of
(A) 3.4
(B) 20
(C) 34
(D) 10
Correct Answer :34

8)
Temin and Baltimore (1975), discovered reverse transcription in
(A) HIV
(B) Rous Sarcoma Virus
(C) Hepatitis B Virus
(D) None of above
Correct Answer :Rous Sarcoma Virus

9) Euchromatin region of chromosomes is considered genetically

active because is this region
(A) Transcription of RNA from DNA takes place
(B) Darker Staining takes place
(C) Lighter staining taker place
(D) All of the above
Correct Answer :-
Transcription of RNA from DNA takes place

10) Diploid number of chromosome is Ophioglossum is.

(A) 1600
(B) 1262
(C) 631
(D) 2400

Correct Answer :1262

11) In Mendel’s trihybrid experiment, F2 Phenotypic ratio was

(A) 9:3:3:1
(B) 27:9:9:9:3:3:3:1
(C) 27:27:27:9:9:9
(D) 9:9:9:3:3:1
Correct Answer : 27:9:9:9:3:3:3:1

12) A type of dominance relation in which the phenotype of heter

ozygote is more
extreme than that of either homozygous parent, is known as
(A) Incomplete dominance
(B) Over dominance
(C) Codominance
(D) None of above
Correct Answer : Over dominance

13) Which one is a test cross

(A) TT x Tt
(B) Tt x Tt
(C) TT x TT
(D) Tt x tt
Correct Answer :Tt x tt

14) The term ‘Crossing Over’ was first introduced by

(A) Bateson & Punnet
(B) Morgan & Castle (1912)
(C) Janssen (1909)
(D) Sutton & Boveri
Correct Answer : Morgan & Castle (1912)

15) According to C.B. Bridges (1925), if the sex index value (X/A)
in drosophila is 0.5 or less then the offspring will be.
(A) Male
(B) Female
(C) Both male and female
(D) None of these
Correct Answer : Male

16) Genes that are carried on the same chromosome are called
(A) Linked Genes
(B) Unlinked Genes
(C) Dominant genes
(D) Recessive Genes
Correct Answer : Linked Genes

17) When the duplicated gene sequence is attached to another po

sition owning to
inter chromosomal duplication then it is known as
(A) Inversion
(B) Transposed Duplication
(C) Direct Tandem Duplication
(D) None of the above
Correct Answer : Transposed Duplication

18) Mutation which occurs during DNA replication is called

(A) Frame Shift Mutation
(B) Copy Error Mutation
(C) Spontaneous Mutation
(D) None of the above
Correct Answer : Copy Error Mutation

19) The Genome D present in bread wheat is supposed to have b

een derived from
(A) Aegilops Squarrosa
(B) Aegilops Speltoides
(C) Triticum Monococcum
(D) None of the above
Correct Answer : Aegilops Squarrosa

20) Split genes are found in

(A) Eukaryotes
(B) Prokaryotes
(C) Eukaryotes and Prokaryotes Both
(D) None of the above

Correct Answer : Eukaryotes

21) Who discovered split gene ?

(A) P. Chambor
(B) Johanssen
(C) P. Leader
(D) P.Sharp and R.Roberts
Correct Answer :P.Sharp and R.Roberts

22) Rate of mutation in nature is

(A) 1x105
(B) 1x108
(C) 1x105
(D) 1x1012
Correct Answer :1x105

23) Artificial synthesis of mRNA was done by ?

(A) M.Nirenberg (1951)
(B) H.G. Khurana
(C) Okha (1955)
(D) F. Griffith
Correct Answer :H.G. Khurana
24)
Role of ‘Single Stranded Binding’ (SSB) protein in DNA replication
is
(A) Separator Primer
(B) Stabilizes Unwounded DNA
(C) Prevents Supercoiling
(D) None of above
Correct Answer :Stabilizes Unwounded DNA

25) Clover leaf model of tRNA was proposed by.

(A) Nirenberg & Mathai
(B) Robert Holley
(C) Okha
(D) H.G.Khurana
Correct Answer :Robert Holley

26)
In DNA replication the Okazaki fragments on the lagging strand a
re joined together by.
(A) Helicase
(B) DNA Ligase
(C) DNA Polymerase
(D) Correct Answer :DNA Ligase

27)
According to Meselson & Stahl experiment of DNA replication, DN
A molecules are
centrifuged in CsCl density gradient, they separate on the basis of
?
(A) Mass Number
(B) Volume
(C) Density
(D) All of above
Correct Answer :All of above

28) In meiosis, chromosomes are reduplicated during

(A) Prophase I
(B) Prophase II
(C) Interphase
(D) Inter Kinesis
Correct Answer :Interphase

29) After a m-
RNA has been translated and leaves the ribosome or polysome, it
is.?
(A) Dehydrated
(B) Degraded
(C) Dehydrolysed
(D) Oxidised
Correct Answer :Degraded

30)
In mitosis, just after duplication, the two sister chromatids remai
n attached at
(A) Spindle
(B) Asters
(C) Centromere
(D) Centrioles

Correct Answer : Centromere

41)The generation of nuclear mRNA from precursor RNA requires

the activity of
(A) Spliceosomes
(B) Endonuclease
(C) Exonuclease
(D) Supersolenoid
Correct Answer :Spliceosomes

42)
Plasmids which may integrate into the bacterial chromosomes are
called
(A) Conjugative Plasmids
(B) RPlasmids
(C) Episomes
(D) FPlasmids
Correct Answer :Episomes

43)
Heritable change in gene expression that does not involve change
in underlying
DNA is termed as
(A) Eugenics
(B) Epigenetics
(C) Phytogenetic
(D) Genetic Engineering
Correct Answer :Epigenetics

44) In Sicklecell syndrome, the amino acid substituted is.

(A) Glutamic acid by valine in chain
(B) Valine by glutamic acid in chain
(C) Glutamic acid by valine in chain
(D) None of the above
Correct Answer :Glutamic acid by valine in chain

45) Which of the following is a recessive trait in garden pea.

(A) Round Seeds
(B) Tall sterm
(C) Wrinkled Seeds
(D) Coloured Seed Coat
Correct Answer :Wrinkled Seeds

46)
Which country was first to begin commercial use of transgenic to
mato & tobacco?
(A) India
(B) America
(C) China
(D) Canada
Correct Answer :China
47) In India Inter-
ministerial committee to decide commercial use of GMO5 is
(A) GEAC
(B) DBT
(C) DST
(D) EPA
Correct Answer :GEAC

48) The first genome sequenced was ?

(A) Haenophilus influence
(B) E. coli
(C) Tobacco
(D) None
Correct Answer :Haenophilus influence

49)
The genetic exchange between different evolutionary linkages is r
eferred to as:
(A) Horizontal gene transfer
(B) Lateral gene transfer
(C) Horizontal gene transfer and Lateral gene transfer both
(D) None of above
Correct Answer :-
Horizontal gene transfer and Lateral gene transfer both

50) Most comprehensive database for SNP5 is:

(A) EMBL
(B) SWISS PROT
(C) HGBASE
(D) Mito

Correct Answer :HGBASE

51) Gene density in human genome is:

(A) One gene per 2 kb
(B) 1 gene per 10 kb
(C) 1 gene per 100 kb
(D) None of above
Correct Answer :1 gene per 100 kb

52)
Process that identifies gene their regulatory sequences and their f
unction is termed as
(A) Synteny
(B) Annotation
(C) PCR
(D) DNA fingerprinting
Correct Answer :Annotation

53) The printed oligonucleotide chips are produced by?

(A) DNA Sequences
(B) Photolithography
(C) 3D printing
(D) None of above
Correct Answer :Photolithography

54) Which of the following is an in-

vivo system to study protein interactions?
(A) Micro arrays
(B) Yeast 2 hybrid system
(C) SDSPAGE
(D) None of above
Correct Answer :Yeast 2 hybrid system

55) Which of the following is not protein sequence database?

(A) SWISSPROT
(B) PDB
(C) dbEST
(D) TrEMBL
Correct Answer :dbEST

56)
The method of developing multilines in wheat was first outlined b
y?
(A) Hull (1945)
(B) Borlaug (1953)
(C) M.S. Swami Nathan
(D) None of these
Correct Answer :Borlaug (1953)

57) Germplasm collection maintained in India at

(A) NBPGR New Delhi
(B) CPRI, Shimla
(C) CRRI, Cuttack
(D) All of above
Correct Answer :All of above

58) Who made the first artificial interspecific plant hybrid?

(A) Borlaug (1953)
(B) T. Fairchild
(C) M.S.Swaminathan
(D) None of the these
Correct Answer :T. Fairchild

59)
Germplasm collections are done largely from a area where agricul
ture is:
(A) Not in practice
(B) Advanced
(C) Advanced
Stille primitive
(D) None of these
Correct Answer :Stille primitive

60)
Combining desirable characters in one plant and then multiplying
it,is called as:
(A) Selection
(B) Tissue culture
(C) Genetic engineering
(D) Crop improvement

Correct Answer :Crop improvement

61) The oldest method of plant breeding is
(A) Introduction
(B) Hybridization
(C) Selection
(D) None of above
Correct Answer :Selection

62)
Method of selection in plants showing vegetative propagation is:
(A) Pedigree selection
(B) Pure line selection
(C) Clonal selection
(D) Mass selection
Correct Answer :Clonal selection

63)
The best way to obtain bacteria and virus free plants through tiss
ue culture is
(A) Micro propagation
(B) Seed germination after gamma irradiation
(C) Shoot tip culture
(D) All of above
Correct Answer :Micro propagation

64)
The hybrid progeny of cytoplasmic male sterile plants would be?
(A) Hermaphrodite
(B) Male sterile
(C) Female sterile
(D) All of these
Correct Answer :Male sterile

65)
The process involving inspection, fumigation and growing the intr
oduced plant material in isolation is known as
(A) Acclimatization
(B) Quarantine
(C) Adaptation
(D) All of above
Correct Answer :Quarantine

66)
If the introduction of any wild species or land cultivation is done, f
or developing genetically improved variety, by selection or cross
hybridization, it is referred to as:
(A) Primary Introduction
(B) Secondary Introduction
(C) Modification
(D) None of the above
Correct Answer :Secondary Introduction

67)
Which of the following varieties of wheat was selected from one o
f the variety of CIMMYT, Mexico) ?
(A) Sonora 64
(B) Lerma roso
(C) Kalyan Sona
(D) All of the above
Correct Answer :Kalyan Sona

68)
Which of the following weeds have been introduced in India from
other countries along with plant materials ?
(A) Argemone Mexicana
(B) Eicchornea crassipes
(C) Parthenium
(D) All of above
Correct Answer :All of above

69) Pure line selection is also called

(A) Inbred selection
(B) Progeny selection
(C) Single line selection
(D) All of above
Correct Answer :All of above

70) Mutation frequency during plant breeding depends on

(A) Dose (concentration × time)
(B) Temperature
(C) pH Value
(D) All of the above

Correct Answer :All of the above

71) Induction of polyploidy in potato is achieved by treating with?
(A) Lanolin Paste
(B) Colchicine
(C) Xrays
(D) None of these
Correct Answer :Colchicine

72) Kufri Alankar, Kufri Chamatkar, upto-

date are important varieties of?
(A) Okra
(B) Capsicum
(C) Potato
(D) Rice
Correct Answer :Potato

73)
Which of the following in not fusarium wilt resistance variety of ch
ick pea?
(A) Radhey
(B) Gaurav
(D) Vijay
Correct Answer :Gaurav

74) A quantitative trait locus (QTL)?

(A) Contains a known gene which affects a polygenic trait
(B) Contains a single gene which affects a polygenic trait
(C) Contrast all of the variation for a polygenic trait
(D) None of these
Correct Answer :None of these
75)
What would be the colors of hulls of wheat plants heterozygous fo
r all three hull colors Loci?
(A) Light red
(B) Dark red
(C) Medium red
(D) All of above
Correct Answer :Medium red

76) Dominance can easily confused with over dominance if?

(A) SNP5 are involved
(B) QTL5 are involved
(C) rDNA is involved
(D) None of these
Correct Answer :QTL5 are involved

77)
When two or more events occur simultaneously. They are called
(A) Independent events
(B) Simple events
(C) Compound events
(D) All of above
Correct Answer :Compound events

78)
When the probability is calculated on the basis of reasoning even
before any trail or experiment it is called?
(A) Mathematical Probability
(B) Apriori Probability
(C) Both the above
(D) None of these
Correct Answer :Apriori Probability

79) PBR322 is an example of

(A) Shuttle Vector
(B) Phage Vector
(C) Plasmid Vector
(D) Cosmid Vector
Correct Answer :Plasmid Vector

80) Which pair is right?

(A) Multi Copy Plasmid
(B) Relaxed Replication
(C) Simple Copy Plasmid
(D) Relaxed Replication
Multiply Plasmid
Stringent Replication
None of these

Correct Answer :Multi Copy Plasmid Relaxed Replication

81) tetr, kanr are example of

(A) Marker genes
(B) Cos Site
(C) Ori site
(D) All of above
Correct Answer :Marker genes

82)
Cosmids are prepared by inserting______of phage into a ______
.
(A) TDNA, Plasmid
(B) CosSite, Plasmid
(C) Genome, Plasmid
(D) None of these
Correct Answer :CosSite, Plasmid

83) Isolation of B+ gene from B thuringiensis was done by ?

(A) Barton et al (1987)
(B) Venter et al (1998)
(C) Baltimore
(D) Braun
Correct Answer :Barton et al (1987)
84) Isozymes analysis of hybrid verification employs
(A) Starch gene
(B) Polyacrylamide gene
(C) Starch gene and Polyacrylamide gene both
(D) None of these
Correct Answer :Starch gene and Polyacrylamide gene both

85)
In Haploid culture, chromosome duplication without nuclear divisi
on taker place it is known as ?
(A) Eumitosis
(B) Endomitosis
(C) Cloning
(D) All of the above
Correct Answer :Endomitosis

86)
Immobilized cell systems uses _______ for cell immobilization.
(A) Calcium alginate
(B) Agarose Beads
(C) Calcium alginate and Agarose Beads both
(D) None of these
Correct Answer :Calcium alginate and Agarose Beads both

87) Intron are absent in ..?

(A) Zein Storage genes of maize
(B) Soybean Protein Genes
(C) Zein Storage genes of maize and Soybean Protein Genes both
(D) None of these
Correct Answer :-
Zein Storage genes of maize and Soybean Protein Genes both

88) Cryopreservation is the storage of material at ultra-

low temperatureby.
(A) Very Rapid Cooling
(B) Gradual Cooling
(C) Gradual Cooling & Simultaneous Dehydration at Low
Temperature
(D) All of the above
Correct Answer : All of the above

89)
Electrophoretic separation of DNA molecules, several megabases i
n size, achieved by ?
(A) PFGE
(B) FISH
(C) SDSPAGE
(D) None of these
Correct Answer :PFGE

90)
Confirmation of DNA transformation in rDNA teachnology can be
done with the help of
(A) Western Blotting
(B) Southern Blotting
(C) PFGE
(D) PCR

Correct Answer :Southern Blotting

91) Which of the following is type I restriction endonuclease?
(A) E co K
(B) E co B
(C) Alu I
(D) E co K and E co B both
Correct Answer :E co K and E co B both

92) Which of the following is DNA modifying enzymes?

(A) Kinase
(B) DNA Polymerase
(C) Alkaline Phosphatase
(D) All of above
Correct Answer :All of above

93) Cloning large piece of DNA can be achieved by

(A) Phase
(B) Cosmids
(C) YAC5
(D) All of above
Correct Answer :All of above

94) Amplification buffer for PCR reaction contains

(A) KCL
(B) Tris CL
(C) 1.5 mm MgCl2
(D) All of the above
Correct Answer :All of the above

95) Co-
dominent marker not used in MAS methot of plant breading is?
(A) SSR
(B) SNP
(C) ASLP
(D) All of the above
Correct Answer :ASLP

96) RFLPQTL linkage relationship can be studied in

(A) Self Pollinated Species
(B) Among Inbred Lines in CrossPollinated Species
(C) Self Pollinated Species and Among Inbred Lines In Cross
Pollinated Species both
(D) None of these
Correct Answer :-
Self Pollinated Species and Among Inbred Lines In Cross
Pollinated Species both

97)
QTLS for root traits are being used to develop high yielding draug
ht resistant in ?
(A) Rice
(B) Maize
(C) Rice and Maize both
(D) None of these
Correct Answer :Rice and Maize both
98) Gene silencing is caused by?
(A) DNA methylation
(B) CoSuppression
(C) Antisense RNA
(D) All of above
Correct Answer :All of above

99) Genes conferring insect resistance to plants are?

(A) Bt gene
(B) ipt gene
(C) Pht gene
(D) All of above
Correct Answer :All of above

100)
Hepatitis B surface antigen has been expressed in tobacco to
(A) Induce Immunity Against HB5 Ag
(B) Induce Hepatitis B in Tobacco
(C) Induce Immunity Against HB5 Ag and Induce Hepatitis B in
Tobacco/HB5 Ag
(D) None of these
Correct Answer :
Induce Immunity Against HB5 Ag/ HB5 Ag