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LWT - Food Science and Technology 44 (2011) 2180e2184

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LWT - Food Science and Technology


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Status of dietary fiber contents in pigmented and non-pigmented rice varieties


before and after parboiling
Y.S. Savitha, Vasudeva Singh*
Department of Grain Science and Technology, Central Food Technological Research Institute, Mysore 570 020, Karnataka, India1

a r t i c l e i n f o a b s t r a c t

Article history: Five different varieties of paddy (four pigmented and one non-pigmented) were shelled and milled in
Received 27 July 2010 pre and post parboiled form, their dietary fiber contents were estimated. Under similar conditions of
Received in revised form milling, raw rice showed a high degree of polish (DOP), 9e12 g/100 g and parboiled rice showed low
24 May 2011
DOP, 4.6e6.6 g/100 g. Dietary fiber content was high in pigmented rice, 9e10 g/100 g compared to non-
Accepted 8 June 2011
pigmented, w6 g/100 g. Soluble fiber content in pigmented head rice (dehusked) varied from 1 to 1.5 g/
100 g and in its brokens varied from 0.45 to 1.45 g/100 g. Dietary fiber content was low by about 1% in
Keywords:
parboiled rice. In the parboiled rice of pigmented varieties, the total fiber content varied from 7.95  0.15
Pigmented rice
Non-pigmented rice
to 9.05  0.25 g/100 g and the soluble fiber content varied from 0.7 to 0.9 g/100 g. In milled parboiled
Parboiling rice the respective values were 5  0.4 to 6  0.1 g/100 g and 0.85  0.05 to 1.25  0.05 g/100 g.
Shelling However, the soluble fiber content in the non-pigmented brown rice, IR-64 remained same after par-
Polishing boiling, 0.75  0.5 g/100 g. Milled parboiled rice showed higher soluble dietary fiber compared to milled
Dietary fiber raw rice. In conclusion, dietary fiber was high in pigmented rice varieties when compared with non-
pigmented rice.
Ó 2011 Elsevier Ltd. All rights reserved.

1. Introduction nutraceuticals) is termed as the degree of milling/polishing, which


determines the whiteness of rice. Paddy is also processed into
Rice (Oryza sativa L.) possibly the oldest domesticated grain parboiled rice, which is consumed as the staple diet by a large
(w10,000 years), is the staple food of half of the world’s population. section of the population, generally in coastal areas of India.
India is the second leading producer of rice in the entire world, Prior to consumption, food grains are generally subjected to
preceded only by China. India’s annual paddy production is around different processes, which alter the bioavailability of both macro
144 million metric tonnes. Among the cereals, rice is mainly and micronutrients as well as other phytochemicals of nutraceut-
consumed in the whole form after appropriate processing. Milling ical value. It is a known fact that during parboiling, starch gets
is a term that describes the process of converting paddy into rice. gelatinized and subsequently a portion of it gets retrograded. This
The basic principle of a rice milling is the removal or separation of ultimately leads to the formation of Resistant Starch (RS), which is
the husk (dehusking) and the bran (polishing) to produce the edible considered as a “better-for-you” carbohydrate. RS is defined as the
portion (endosperm) for consumption. The extent of losses on the sum of starch and remnants of starch gradation not absorbed in the
edible portion of the grain during milling depends on many factors intestine. The linear and branched polymers of starch, degree of
such as variety of paddy, method of harvesting and drying, mois- gelatinization, hydrothermal treatment, cooling and storage e all
ture content of paddy during milling, degree of milling, the kind of affect the RS content in foods (Goni, Garcia-Diz, Manas, & Saura-
rice mill used etc. Finally during the milling of paddy (rough rice), Calixto, 1996). In cereal products RS fraction is not digestible both
the husk (hull), milled rice (the edible portion), a mixture of bran in vitro and in vivo (Sajilata, Rekha, & Pushpa, 2006). Native starch,
and germ and brokens are obtained. Milling is usually done when retrograded amylose, amylose lipid complex, encapsulated gelati-
paddy is dry about 14 g/100 g of moisture content. The extent of nized starch are 4 types of RS. RS is nutritionally an important
removal of the bran layers (which is a treasure house of several fraction of dietary starch, which akin to dietary fiber components,
escapes digestion and absorption in the small intestine, but later
gets fermented by the gut micro-flora. As a result of microbial
* Corresponding author. Tel.: þ91 821 2510843; fax: þ91 821 2517233.
degradation of RS in the colon, an increase in short-chain fatty acids
E-mail address: singhva2003@yahoo.co.in (V. Singh). (especially butyrate) takes place. This has been studied and found
1
Council of Scientific and Industrial Research, New Delhi to stabilize colonic cell proliferation as a preventive mechanism for

0023-6438/$ e see front matter Ó 2011 Elsevier Ltd. All rights reserved.
doi:10.1016/j.lwt.2011.06.004
Y.S. Savitha, V. Singh / LWT - Food Science and Technology 44 (2011) 2180e2184 2181

colon cancer (Englyst, Kingman, & Cummings, 1992). These are All these paddy varieties were harvested in the month of JuneeJuly
lower in acetate and higher in butyrate than those of conventional 2008 and were cleaned and stored at 4e6  C until use. Pepsin,
fibers. The formation of RS during the processing of starchy foods is pancreatin, a- amylase (heat stable), were procured from M/s Sigma
a new tool for creating fiber-rich foods. Chemical Co., St. Louis, MO, USA. All other chemicals used were of
Generally rice is consumed in a highly polished form, despite analytical grade.
the valuable nutrient content of brown rice (unpolished or polished
to a lower degree). Brown rice is a good contributor of fiber in the 2.2. Methods
diet. The fiber is one of the seven nutritional factors such as
carbohydrate, protein, fat, vitamin, minerals and water. It has been 2.2.1. Shelling/dehusking
verified by a number of researches and investigations in epidemi- A weighed sample (100 g) of paddy from each variety was
ology that rice dietary fiber has an important physiological effect in dehusked using the Laboratory Satake Sheller (Satake corporation
maintaining health. It also helps prevent the formation of gall Tokyo, Japan) with rubber rolls adjusted to grain size so as to
stones, eliminates undesirable metal ions, decrease of blood serum, minimize the breakage. The shelled rice and husk were weighed
wading the fat disease, lowers the probability of constipation and separately for determining the yield of brown rice/dehusked rice.
intestine cancer. Dietary fiber (DF) is a non-starch polysaccharide
complex that comes from the edible parts of plants or analogous 2.2.2. Milling/polishing
carbohydrates that is resistant to digestion and absorption in the The shelled rice (150 g) of each variety was subjected to milling/
human small intestine and undergoes complete or partial polishing using a Mc-Gill miller for 2 min with a pressure of
fermentation in the large intestine. Dietary fiber can be classified 88.85 kPa. It consists of a wire-mesh screen with slotted perforated
into two major components on the basis of solubility: a). soluble enclosure in which a shaft rubs the grain against the casing (fric-
components, such as pectins, gums and b-glucans; and b). insoluble tional). The bran is removed from the dehusked rice which passes
components, which include cellulose, lignin and hemicelluloses through these screens.
(Happi Emaga, Robert, Ronkart, Wathelet, & Paquot, 2008;
Nawirska & Kwasniewska, 2005; Vergara-Valencia, Granados- 2.2.3. Broken rice
Perez, Agama-Acevedo, Tovra, Ruales, & Bello-Perez, 2007). Insol- The milled rice produced is a mixture of head rice and brokens.
uble fiber adds bulk to the stools and speeds up the passage of Grading of milled rice into different categories, such as small and
stools through the colon. Soluble fiber helps in keeping blood sugar large brokens and head rice was determined manually using rect-
levels stable, and it also creates a feel of satiety and prevents angular perforated sheets with dimpled type holes of
overeating. An Indian adult may consume 50e120 g dietary fiber/ diametere3 mm, 4 mm and 5 mm respectively. These perforated
day depending upon the type and amount of cereal/millet used sheets have been used to separate different sized brokens.
(Navitha & Sumathi, 1992), with a desirable level of 40 g/day
(Narasinga Rao & , 1989). 2.3. Parboiling
Commercially more than two thousand varieties of rice are
grown around the world (Deepa, Singh, & Akhilender Naidu, 2008). The paddy (0.5e2 kg) was soaked in previously boiled (w98  C)
There are red and black paddy varieties which have about 38% more tap water (2e3 L) and the mix was stirred, floating chaff removed
protein, about 18% more crude fiber and are richer in lysine, vitamin and the vessel covered with jute sacks and left overnight (Sashikala,
B1 and other minerals compared to conventional varieties Singh, & Ali, 2005). The water was drained off and the soaked paddy
(Chaudhary & Tran, 2001). Colored rice (black and red) consump- spread on small wire-mesh trays, and steamed in an autoclave at
tion is rapidly growing due to their healthy functional food ingre- open atmospheric pressure for 20 min. The paddy was then air-
dients (Kim et al., 2008). Anthocyanins, a group of reddish or purple dried in the shade (1e2 days) to a safe moisture level. Parboiled
flavonoids, are reported to be the primary pigments in these rice paddy obtained was dehusked and milled under similar conditions
varieties (Abdel & Hucl, 1999; 2003). Anthocyanins have been as mentioned above (2.2.1 & 2.2.2).
recognized as health-promoting functional food ingredients due to
their antioxidant activity, anticancer, hypoglycemic and anti- 2.4. Determination of soluble/insoluble dietary fiber
inflammatory effects. At present, red rice is commonly used as
a functional food ingredient in bread, ice cream and liquor (Kim All samples of raw (dehusked head rice and brokens, polished
et al., 2008). Anthocyanins are located in outer layers of the rice head rice and brokens) and parboiled (dehusked and polished head
kernel, which could be separated into anthocyanin-rich fractions rice) were pulverized in a mixer (Johnson Lady Bird plus), and the
for use as functional colorants or functional food ingredients. flour was passed through 60-mesh sieve (250 mm). The flour was
Information on the changes in the total dietary fiber contents gravimetrically defatted prior to fiber analysis by extracting with
before and after parboiling of red rice varieties is limited and petroleum ether (60e80  C) for 12e16 h using a Soxhlet apparatus
scanty. Hence the present study was aimed at bridging this gap (AOAC, 2000). Total dietary fiber was analyzed as per Asp,
with an objective to study the effect of varietal difference of rice Johansson, Hallmer, and Silgestrom (1983) with slight modifica-
subjected to parboiling and their shelling and milling in raw and tions. Reagents used were 0.1 mol/L sodium phosphate buffer, pH
parboiled form. It also aims at determining the total dietary fiber 6.0; 4 mol HCl/L; 4 mol/L NaOH; 95% of ethanol (95 mL of
content in pigmented and non-pigmented rice. ethanol þ 5 mL of water) and 78% of ethanol (78 mL of
ethanol þ 22 mL of water). The procedure in brief is as follows:
2. Materials and methods About one gram of defatted sample was suspended in 25 mL of
0.1 mol/L sodium phosphate buffer (pH 6.0) and treated with100 mL
2.1. Materials of a- amylase at boiling water bath temperature for 15 min, and
cooled to room temperature. About 20 mL of distilled water was
Four red-pigmented paddy varieties viz. Jyothi, Aishwarya, Kar added and the pH was adjusted to 1.5 with 4 mol HCl/L. About
bhatha (medium) and Kar bhatha (small) and one non-pigmented 100 mg of pepsin was added and incubated at 40  C in a water bath
variety, IR-64 were procured from the Agriculture Produce with agitation for 60 min. Twenty milliliters of distilled water was
Market committee (APMC), Bandipalya, Mysore, Karnataka, India. added and the pH was adjusted to 6.8 with 4 mol/L NaOH. The
2182 Y.S. Savitha, V. Singh / LWT - Food Science and Technology 44 (2011) 2180e2184

Fig. 1. Photographs of different parts of paddy. A: Jyothi (Pigmented) and B: IR-64 (non-pigmented); (a). Paddy, (b). Husk, (c). Dehusked grains, (d). Polished grains and (e). Bran.

electrode was rinsed with a few milliliters of water. About 100 mg 2.5. Statistical analysis
of pancreatin was added and incubated at 40  C in a water bath
with agitation for 60 min. The pH was adjusted to 4.5 with 4 mol Data were analyzed using Graph Pad Instat statistical software
HCl/L. The contents were filtered through a dried and weighed (Graph Pad Software, Inc., La Jolla, CA, USA). Each experiment was
crucible (porosity 2) containing 0.5 g of dry Celite as the filter aid. performed in triplicate and the results were expressed as the mean
values  standard deviation. Results were analyzed and signifi-
(a) Residue (insoluble Fiber): Washed with 2  10 mL of distilled cance level was calculated using TukeyeKramer multiple compar-
water and again washed with 2  10 mL of 95% of ethanol ison test by means of one way ANOVA. Values with p < 0.05 were
(95 mL of ethanol þ 5 mL of water), it was dried at 105  C to considered statistically significant.
constant weight (or overnight). After cooling it in a desiccator
(D1), the residue was incinerated at 550  C for at least 5 h. After 3. Results and discussion
cooling in a desiccator (I1) it was weighed again.
(b) Filtrate (soluble Fiber): The volume of the filtrate was adjusted to Photographs of different parts of pigmented and non-pigmented
100 mL. Four hundred milliliters of warm (60  C) 95 g/100 g of paddy are depicted in Fig.1. The husk content in pigmented and non-
ethanol was added. It was allowed to precipitate for 1h (time pigmented rice varieties varied from 21.8 to 23.3 g/100 g, dehusked
could be shortened). It was then filtered through a dried and grains varied from 76.7 to 78.1 g/100 g. Variation was observed in
weighed crucible (porosity 2) containing 0.5 g of dry Celite. head rice content among pigmented varieties, w 41e64 g/100 g.
Washed with 2  10 mL of 78% of ethanol (78 mL of Head rice recovery was least in Jyothi, 41 g/100 g and highest in
ethanol þ 22 mL of water), 2  10 mL of 95% of ethanol (95 mL of Aishwarya w 64 g/100 g, indicating the higher susceptibility of the
ethanol þ 5 mL of water). It was dried at 105  C overnight, grain for breakage in Jyothi w37 g/100 g and least in Aishwarya
weighed after cooling in a desiccator (D2), incinerated at 550  C w13 g/100 g among the pigmented varieties. Brokens in other two
for at least 5 h and then weighed after cooling in a desiccator (I2). pigmented varieties were w20 and w25 g/100 g respectively. This is
clearly shown in the bar diagram (Fig. 2).
Blank: Insoluble and soluble blank values were obtained by After parboiling, the husk content reduced by 1e2% i.e., from
running the procedure without sample (B1 and B2). The blank 21e23 to 19e22 g/100 g in comparison to raw husk contents.
values were checked occasionally. Dehusked grains increased from 78.1 to 80.5 g/100 g as shown in
Calculation: Fig. 3. This is because parboiling maximizes the milling yield by
minimizing the breakage. Grains become harder after the process of
D1  I1  B1
Insoluble dietary fiberðg=100gÞ ¼  100 parboiling. The starch gets completely gelatinized, apparently
W
sufficient solubilization takes place in order to “cement” these
cracks together, thus strengthening the kernel (Priestley, 1976) and
D2  I2  B2
Soluble dietary fiberðg=100gÞ ¼  100 hence lesser breakage in the process of dehusking of parboiled
W
paddy.
Where, W ¼ sample weight (g), D ¼ weight after drying (g), Under similar conditions of polishing, raw rice showed high DOP
I ¼ weight after Incineration (g) and B ¼ weight of ash free (9e12 g/100 g) and parboiled rice showed low DOP (4.6e6.6 g/
blank (g). 100 g). This is attributed to the difficulty in the removal of bran
Y.S. Savitha, V. Singh / LWT - Food Science and Technology 44 (2011) 2180e2184 2183

Table 1
Dietary fiber of dehusked head rice of pigmented (Jyothi, Aishwarya, Kar Bhatha,
medium and small) and non-pigmented, (IR-64) rice varieties before and after
parboiling (g/100 g).

Rice varieties Insoluble fiber Soluble fiber Total fiber


Jyothi RDHR 7.40  0.10a 1.50  0.10a 8.90  0.20a
PDHR 7.25  0.15A 0.70  0.00A 7.95  0.15A
Aishwarya RDHR 9.25  0.05b 0.80  0.10b 10.05  0.15b
PDHR 8.15  0.05B 0.90  0.20B 9.05  0.25B
Kar Bhatha (Medium) RDHR 8.35  0.15c 0.80  0.10cb 9.15  0.25a
PDHR 7.65  0.05C 0.75  0.15A 8.40  0.20C
Kar Bhatha (Small) RDHR 8.00  0.20dc 1.00  0.10dbc 9.00  0.10a
PDHR 7.80  0.10DC 0.75  0.15A 8.55  0.05DC
IR-64 RDHR 5.40  0.30e 0.75  0.05ebc 6.15  0.25c
PDHR 4.90  0.10E 0.75  0.05A 5.60  0.10E
(ae)
RDHR: Raw Dehusked Head Rice; (AE) PDHR: Parboiled Dehusked Head Rice.
Values are mean  standard deviation of three determinations (n ¼ 3).
Values within the same column with different letters are significantly different at
p < 0.05.

and IR-64 (1.00  0.10, 0.80  0.10 and 0.75  0.05 g/100 g)
respectively.
Fig. 2. Shelling and milling properties of pigmented (aed) and non-pigmented (e) rice
On parboiling, the dietary fiber contents reduced to a different
varieties (raw). Pigmented- (a: Jyothi, b: Aishwarya, c: Kar Bhatha (Medium), d: Kar
Bhatha (Small); Non-Pigmented- (e: IR-64)).
extent in each of the varieties. During parboiling, starch gelatinizes
and while drying retrogradation of gelatinized starch takes place,
where linear polymers of glucose with alpha (1/4) linkage precip-
layers from parboiled rice, as it becomes hard in nature while itates (Bhattacharya & Indudhara Swamy, 1967; Mahadevamma &
parboiling and drying process. The hardness of grains is greatly Tharanathan, 2007). The range of decrease in dietary fiber content
affected by the conditions of parboiling like moisture content, the varied from 5 to w11% among pigmented varieties. The extent of
extent of gelatinization and retrogradation (Islam, Shimizu, & decrease was high in Jyothi w11% and that of non-pigmented par-
Kimura, 2004). boiled rice was w9% as shown in Table 1.
Total dietary fiber content in raw dehusked head rice ranged Parboiling reduced the soluble fiber to a greater extent,
from w 8.9e10 g/100 g in pigmented rice, whereas in non- compared to raw rice. In case of Jyothi, about 53% reduction in
pigmented rice it was around 6 g/100 g as reported in Table 1. soluble fiber and in Kar bhatha (small), 25% reduction occurred. In
Among the raw dehusked pigmented rice, Aishwarya showed the other varieties, the extent of decrease was 6e12%. The soluble fiber
highest dietary fiber, w10 g/100 g followed by Kar bhatha in the non-pigmented brown rice remained same, 0.75  0.05 g/
(medium), Kar bhatha (small) and Jyothi (9.15  0.25, 9.0  0.10 and 100 g after parboiling. In the case of Aishwarya, the soluble fiber
8.90  0.20 g/100 g) respectively. content was high by 0.1%, which may be because of change in the
Soluble fiber varied from 0.75 to 1.5 g/100 g in the raw dehusked degree of polish in parboiled rice under similar conditions of
rice varieties. The highest was noticed in Jyothi, 1.50  0.10 g/100 g milling.
followed by kar bhatha (small), Aishwarya, Kar bhatha (medium) Total dietary fiber content in polished rice (raw) was almost 4 g/
100 g in both pigmented and non-pigmented rice varieties, indi-
cating heavy loss of fiber after polishing/milling. However, in par-
boiled polished rice the fiber contents were high (Table 2) ranging
from 4.5 to 6 g/100 g in pigmented and non-pigmented rice vari-
eties. Parboiling, a process carried out on paddy, results in signifi-
cant property changes of the grain (Ali & Bhattacharya, 1976).
Parboiling increases RS production. Higher contents of RS have
been reported in parboiled rice than in raw milled rice. The RS

Table 2
Dietary fiber of polished head rice of pigmented (Jyothi, Aishwarya, Kar Bhatha,
medium and small) and non-pigmented, (IR-64) rice varieties before and after
parboiling (g/100 g).

Rice varieties Insoluble fiber Soluble fiber Total fiber


Jyothi RPHR 3.20  0.10a 1.00  0.10a 4.20  0.00a
PPHR 4.35  0.35A 0.85  0.05A 5.20  0.40A
Aishwarya RPHR 2.90  0.20b 1.25  0.05a 4.15  0.15a
PPHR 4.20  0.30A 1.25  0.05B 5.45  0.25A
Kar Bhatha (Medium) RPHR 3.20  0.00ad 0.90  0.10abd 4.10  0.10a
PPHR 5.05  0.05B 0.90  0.20AB 5.95  0.15BA
Kar Bhatha (Small) RPHR 3.05  0.05abd 0.90  0.20acd 3.95  0.15a
PPHR 5.05  0.25CB 0.95  0.15AB 6.00  0.10CAB
IR-64 RPHR 2.90  0.00cba 1.05  0.05a 3.95  0.05a
PPHR 3.45  0.15D 1.05  0.15AB 4.50  0.00D
Fig. 3. Shelling and milling properties of pigmented (aed) and non-pigmented, (e) rice
(ae)
varieties (parboiled). Pigmented- (a: Jyothi, b: Aishwarya, c: Kar Bhatha (Medium), d: RPHR: Raw Polished Head Rice; (AE) PPHR: Parboiled Polished Head Rice.
Kar Bhatha (Small); Non-Pigmented- (e: IR-64)). Note: In case of Parboiled rice, the Values are mean  standard deviation of three determinations (n ¼ 3).
brokens are negligible in all the varieties viz: Jyothi: 0.04, Aishwarya: 0.23, KB (M): Values within the same column with different letters are significantly different at
0.05, KB(S): 0.02, IR-64: 0.07 g/100 g are shown by the arrow in the fig. p < 0.05.
2184 Y.S. Savitha, V. Singh / LWT - Food Science and Technology 44 (2011) 2180e2184

Table 3 health-promoting functional food ingredients due to their antiox-


Dietary fiber of dehusked and polished broken rice of pigmented (Jyothi, Aishwarya, idant activity. Thus, there should be future nutritional recommen-
Kar Bhatha, medium and small) and non-pigmented, (IR-64) rice varieties (raw) (g/
100 g).
dations for red-rice for the targeted/general population taking into
account the notion of glycemic index (GI).
Rice varieties Insoluble fiber Soluble fiber Total fiber
Jyothi RDBR 6.95  0.05a 1.45  0.05a 8.40  0.00a
RPBR 3.60  0.30A 1.35  0.15A 4.95  0.15A Acknowledgements
Aishwarya RDBR 8.25  0.15b 0.70  0.10b 8.95  0.05b
RPBR 3.95  0.05A 1.00  0.20A 4.95  0.25A
Kar Bhatha (Medium) RDBR 7.15  0.05a 0.45  0.15cb 7.60  0.00c
The authors thank Dr. V. Prakash, Director, Central Food Tech-
RPBR 3.15  0.05B 1.35  0.05A 4.50  0.10B nological Research Institute, Mysore, India, for his support in the
Kar Bhatha (Small) RDBR 7.00  0.00a 1.20  0.10a 8.20  0.10d present study. Mr. S. R. Pradeep for helping in statistical analysis.
RPBR 3.95  0.15A 1.15  0.15AD 5.10  0.00A Ms. Y. S. Savitha, CSIR-SRF, wishes to gratefully acknowledge the
IR-64 RDBR 4.20  0.10c 0.70  0.10dbc 4.90  0.00e
financial support from CSIR, New Delhi, in carrying out this
RPBR 2.15  0.05C 0.95  0.15BACD 3.10  0.20C
investigation.
(ae)
RDBR: Raw Dehusked Broken Rice (AE) RPBR: Raw Polished Broken Rice.
Values are mean  standard deviation of three determinations (n ¼ 3).
Values within the same column with different letters are significantly different at
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