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An enzyme look more like a tangled chain of amino acids that strung up together. If it
zoomed even further you’d see tons of tons of atoms. And without this atoms s staying in the right
spot the substrate would not be able to fit in the active site of the enzyme. So in what conditions
do the atoms actually stay put and keep their shape and in what conditions do they fail to do so?
There are three conditions that affect this the most: the effect of temperature, pH and the substrate
concentration.
When enzyme and substrate molecules are mixed they randomly collide forming enzyme
substrate complexes. Like most chemical reactions, as temperature increases so does the kinetic
energy of the molecule and therefore also the rate at which these collisions occur. Optimum
temperature is the temperature at which the maximum rate of reaction is achieved. A ten-degree
Centigrade rise in temperature will increase the activity of most enzymes by 50 to 100%.
in the results. If the temperature continues to increase beyond the optimum temperature, the
enzymes begin to lose their three-dimensional structure and becomes denature, therefore reducing
40°C, most enzyme determinations are carried out somewhat below that temperature.
Over a period of time, enzymes will be deactivated at even moderate temperatures. Storage
of enzymes at 5°C or below is generally the most suitable. Some enzymes lose their activity when
frozen.
Enzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme
is most active - is known as the optimum pH. Extremely high or low pH values generally result in
complete loss of activity for most enzymes. This is graphically illustrated in the graph below.
result of adding hydrogen ions H+. Either of these can potentially alter the charges on the amino
acids that make up the enzyme and this can lead to the enzymes active site changing shape because
the bonding changes for example and that could denature the enzyme.
The optimum pH value will vary greatly from one enzyme to another, as Table I shows:
Enzyme pH Optimum
Urease 7.0
Invertase 4.5
Catalase 7.0
When the substrate concentration change, it is assumed that the enzyme concentration remains
constant. Initially, increasing substrate concentration from zero will increase the rate of reaction
as the substrate randomly collide with the enzymes and the enzymes catalyze the chemical
reaction. However, there comes a point where all of the enzyme active sites are filled and the
enzymes are already working as quickly as they can to catalyze the reaction, this is where the
maximum rate of reaction is reached and any further addition of substrate beyond this will not
References
Introduction to Enzymes. (n.d.). Retrieved June 19, 2017, from http://www.worthington-
Honey & Acetic Acid Research Proposal | Fixation ... (n.d.). Retrieved June 19, 2017, from
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