Book of Abstracts, 2018

10th CMAPSEEC:
BOOK OF ABSTRACTS
10th Conference on Medicinal and Aromatic Plants of
Southeast European Countries
May 20-24, 2018, Split, Croatia

10th CMAPSEEC
10th Conference on Medicinal and Aromatic Plants
of Southeast European Countries
Jointly organized by:
Association for Medicinal and Aromatic Plants of Southeast European

Countries (AMAPSEEC), Belgrade, Serbia
and
University of Zagreb, Faculty of Agriculture, Zagreb, Croatia
Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
Centre of Excellence for Biodiversity and Molecular Plant Breeding
(CroP-BioDiv), Zagreb, Croatia
Under the patronage of:
Ministry of Agriculture of the Republic of Croatia, Zagreb, Croatia
Ministry of Science of the Republic of Croatia, Zagreb, Croatia
Academy of Agricultural Sciencies, Zagreb, Croatia
Editors
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Technical Editor
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ISBN: 978-953-7878-82-5
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Further information:
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University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology
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10th CMAPSEEC
Dear Friends and Colleagues,

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of Agriculture, Zagreb, Croatia, Institute for Adriatic Crops and Karst Reclamation,
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10th CMAPSEEC
President of the Organizing Commitee

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Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
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President of the Association for Medicinal and Aromatic Plants of South-
east European Countries (AMAPSEEC), Belgrade, Serbia
University of Belgrade, Faculty of Agriculture, Belgrade, Serbia
Members
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Martina Grdiša, Zagreb, Croatia .ODXGLMD&DURYLü6WDQNR=DJUHE&URDWLD
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Tatjana Klepo, Split, Croatia 0LOHQ,*HRUJLHY3ORYGLY%XOJDULD
7RQND1LQþHYLü6SOLW&URDWLD Elvira Gille, Piatra Neamt, Romania
0DUNR5XQMLü6SOLW&URDWLD Martina Grdiša, Zagreb, Croatia
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Filip Varga, Zagreb, Croatia Alban Ibraliu, Tirana, Albania
0DMDäXSDQ3RXU=DJUHE&URDWLD Tatjana Klepo, Split, Croatia
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I. MEDICINAL AND AROMATIC PLANTS ECOLOGY,
DIVERSITY AND ETHNOBOTANY
Monday, May 21st
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25
Ethnobotany of the Adriatic Islands in Croatia
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Chloroplast DNA Diversity and Phylogeography of 6DOYLDRI¿FLQDOLVL. and 26
6DOYLDODYDQGXOLIROLD Vahl
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28
Wild Labiates Aromatic Plants and their Bee Pollinators
0DULMD-XJ'XMDNRYLü,YDQDâROLü0DUWLQD*UGLãD/XNDV]/XF]DM)LOLS9DUJD
29
The Use of Medicinal Plants in the Knin Area, Croatia
'HPXVK%DMUDNWDUL%LOMDQD%DXHU=RUDQ.DYUDNRYVNL/XO]LP=HQHOL
Environmental Pollution and Heavy Metals Accumulation in Salix alba L. 30
(Salicaceae), Along the River Stream of Sitnica
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31
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Structure of 6DOYLDEUDFK\RGRQ Vandas (Lamiaceae) Population
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27
Wild Raspberry from the Central Balkan: From Ethnobotany to Bioactivity
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32
Microsatellite Diversity of Dalmatian Sage Populations from the Balkan
and Apennine Peninsulas
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Medicinal Plants as a Food Source for Pollinators – Fluctuation of 33
Attractiveness of Selected Species During the Day
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34
Genetic Diversity of Immortelle (+HOLFKU\VXP LWDOLFXP /Roth/ G. Don)
Populations in Croatia
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3HJJ\/HRQWDULWRX)RWLQL/DPDUL*ULJRULV,DWURX9DVLOLV3DSDVRWLURSRXORV
Genetic Diversity of Natural Populations of 6DOYLDIUXWLFRVD Mill. and Salvia 35
SRPLIHUD L. from Peloponnese Based on SSR Markers
Christian Zidorn
36
Chemosystematics of the &LFKRULHDH Tribe of the Asteraceae Family
øVPDLOùHQNDUGHú*L]HP%XOXW$KPHW'R÷DQ(UWDQ7X]ODFÕ
37
An Analysis on Wild Edible Plants of the Turkish Asteraceae Taxa
Martina Grdiša )LOLS 9DUJD 0DULMD -XJ'XMDNRYLü 7RQND 1LQþHYLü =ODWNR
/LEHU,YDQ5DGRVDYOMHYLü0DUWLQD%LRãLü=ODWNRâDWRYLü
38
Genetic Background of Dalmatian Pyrethrum (7DQDFHWXPFLQHUDULLIROLXP /
Trevir. /Sch. Bip.) Insecticidal Potential
Filip Varga,YDQ5DGRVDYOMHYLü0DUWLQD*UGLãD=ODWNRâDWRYLü
Predicting Suitable Habitat for Dalmatian Pyrethrum (7DQDFHWXP 39
FLQHUDULLIROLXP /Trevir. /Sch. Bip.) in Croatia
Poster Section M-1 (Monday, May 21st,14:30-15:30)

P-1 Marjol Meço, Alfred Mullaj
Assessing the Medicinal and Aromatic Plants in Watershed of the Middle Section of 40
Devolli River
P-2 *L]HP%XOXW$KPHW'RJDQ,VPDLO6HQNDUGHV5RMGD$YFÕ(UWDQ7X]ODFÕ
The Medicinal and Wild Food Plants of Batman City Center and Kozluk District 41
(Batman-Turkey)
P-30DULQD0DþXNDQRYLü-RFLü6QHåDQD-DULü
Medicinal Plants Traditionally Used in the Treatment of Diabetes in the Region of 42
Suva Planina Mountain (Southeastern Serbia)
P-4 Biljana Bauer%LOMDQD&KXOHYD$QD.RFHYVND
Ethnomedicinal Study of Preparations Obtained from -XJODQGLVLPPDWXULIUXFWXV 43
from the Folk and Traditional Medicine of Republic of Macedonia
P-5 9LNWRULD0DULQRYD
Demographic Characteristics of the Local Population in Certain Settlements on the 44
North Black Sea Coast (Bulgaria) in the Use of Medicinal Plants
P-6 Violeta Dimitrova, Alexander Tashev
Investigation of Medicinal Plants on the Territory of Petrohan Training and 45
Experimental Forest Range (Western Stara Planina, Bulgaria)
P-7 øVPDLOùHQNDUGHú$KPHW'R÷DQ*L]HP%XOXW
3UHOLPLQDU\6WXG\RIWKH)RON0HGLFLQDO3ODQWV8VHGLQ7DúN|SU.DVWDPRQX 46
Turkey)
P-8*HRUJLD)DVVRX*UHJRULV,DWURX)RWLQL/DPDULVassilis Papasotiropoulos
Genetic Diversity of +HOOHERUXVRGRUXV Waldst. & Kit. Subsp. &\FORSK\OOXV (A. 47
Braun) Maire & Petitm. Populations in Greece
P-9 0DUNR66DERYOMHYLü0LORUDG9XMLþLü0DULMDûRVLü$QHWD6DERYOMHYLü
48
Some Insights into Properties of %U\RSK\WHV as Neglected Medicinal Plants
P-10 Petya Boycheva
Ethnobotany of Medicinal Plants Used in Some Parts of the Northern Black Sea 49
Coast Region (Bulgaria)
P-11(OLQD<DQNRYD7VYHWNRYD,YDOHQD,OLHYDStoyan Stoyanov, Boriana Sidjimova,

Marina Stanilova
50
Reproductive Biology of the Endangered Bulgarian Endemic &HQWDXUHDDFKWDURYLL
Urum. (Asteraceae)
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51
Anatomical and Micromorphological Investigations of $UWHPLVLDDUERUHVFHQV L. -
Wild-Growing Medicinal Plant from Montenegro
P-13 Seçil Karahüseyin$\QXU6DUÕ

52
Plants Traditionally Used as Antitussives in Turkey
P-14 Ahmet Dogan

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(Tunceli-Turkey)
P-15 /DLPDâYHLVW\Wơ$OHVLD.UXFKRQRN.ULVWLQD/RåLHQơ
Feverfew 7DQDFHWXPSDUWKHQLXP (L.) Sch. Bip. – Native Species from South East 54
Europe Used in Lithuanian and Belorussian Traditional Medicine
P-16 Ibraliu Alban*L[KDUL%HOXO.DGLDVL1DMDGD$JDVWUD$UOLQGD7DNR5HD+\VNR

Jonida
55
6LGHULWLVUDHVHUL Boiss. & Heldr. (Çaji i Malit) Distribution in Albania, Challenges for
the Future
P-17 Marina Stanilova, Svetlana Bancheva, Malina Delcheva, Stoyan Stoyanov, Boriana
6LGMLPRYD%RU\DQND7UD\NRYD
56
,Q6LWX Conservation of Three Endemic 9HUEDVFXP Species in Bulgaria by Population
Reinforcement
P-18 ,YDQD9LWDVRYLü.RVLü0DULMD-XJ'XMDNRYLü.DWLMD'ROLQD0LUMDQD-HULþHYLü
àXNDV]àXF]DM 57
Plants Used in Traditional Alcoholic Beverages of the Adriatic Islands (Croatia)
P-19 Ina Aneva, (NDWHULQD.R]XKDURYD3HWHU=KHOHY6LPHRQ/XNDQRY0DULD3HQHYD

Medicinal Plants in the Intensively Managed Agricultural Landscape in Pazardzhik - 58
Plovdiv Region of Bulgaria
P-20 %RULV/D]DUHYLü.ODXGLMD&DURYLü6WDQNR
(IIHFWRI$UEXVFXODU0\FRUUKL]DHRQ3KRVSKRUXV'H¿FLW6WUHVV'XULQJ(DUO\ 59
Development Stage of Basil (2FLPXPEDVLOLFXP L.)
P-21 Peter Zhelev,QD$QHYD$QWRDQHWD7UHQGD¿ORYD0LOND7RGRURYD

60
Genus ,QXOD L. in Bulgaria – Distribution and Natural Habitats
P-221DHOD&RVWLFă$QL‫܈‬RDUD6WUDWX,ULQD%R]Elvira Gille
Comparative Anatomy, Micromorphology and Biochemistry of 6DPEXFXVQLJUD L. 61
Fruits
P-23 Yunus Dogan, Anely Nedelcheva
62
0HGLFLQDO%RWDQLFDO.QRZOHGJH$PRQJWKH/DVW5HPDLQLQJ<|UNVRIWKH%DONDQV
P-24
,YDQâRãWDULü=ODWNR/LEHU%RMDQD5DGXORYLü=RUD'DMLü6WHYDQRYLü=ODWNRâDWRYLü 63
Assessment of Population Diversity in Wild 5XEXVLGDHXV L. Populations from Serbia
P-259LãQMD2UHãþDQLQâWH¿FD)LQGUL*XãWHN
64
The Treatment of Menorrhagia with Bioapigyn Herbal Ointment and Pessaries
P-26 Agastra Arlinda, Gixhari Belul, Ibraliu Alban
Evaluation of the Diversity of Common Juniper (-XQLSHUXV&RPPXQLV L.) from the 65
Tippical Geographic Areas in Albania
P-27 Pietro Fusani1LFROD$LHOOR/XLVD3DOPLHUL6WHIDQR3LD]]D6WHIDQ0DUWHQV
)DEUL]LR6FDUWH]]LQL
Morphological, Agronomic and Genetic Characterization of 5KRGLRODURVHD L. 66
Accessions from the Province of Trento, as Preliminary Results for a Study on the
Biodiversity of the Species
P-28 6DLIXOODK1DLNDQGLVK
Essential Oil Extract, Determination of Nano-Emulsions Characteristic and 67
Allelopathic Potential of Medicinal Plant 'XFURVLDDQHWKLIROLD (Dc.) Boiss
P-29 Ina Aneva0LOHQD1LNRORYD3HWHU=KHOHY.DW\D*HRUJLHYD
68
Population Status and Habitats of $OOLXPXUVLQXP L. in Bulgaria
P-30 Ina Aneva(OLQD<DQNRYD3HWHU=KHOHY5R]DOLD1LNRORYD
Population Status and Habitat Characteristic of Two 6LGHULWLV Species in Bulgaria 69
and Greece
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70
Traditional Herbal Teas Consumed in Mersin (Turkey)
P-32 Svetlana Bancheva
71
Ethnobotanical Survey of Medicinal Plants from Asteraceae in Bulgaria
P-33 .ODXGLMD&DURYLü6WDQNR=ODWNR/LEHU)LOLS9DUJD0DUWLQD*UGLãD0LKDLOR5LVWLü
=ODWNRâDWRYLü
72
How Do Morphotypes and Chemotypes Relate to Genotypes? Intraspecies Diversity
of 2FLPXPEDVLOLFXP L.
P-34 ,YDQDâROLü)LOLS9DUJD0DULMD-XJ'XMDNRYLü/XNDV]/XF]DM0DUWLQD*UGLãD
Traditional Preparation of Syrups, Marmalades, Liqueurs and Herbal Grappas in 73
the Knin Area, Croatia
Tour of Split
II. PHYTOCHEMICAL ANALYSIS, PHARMACOLOGY,
BIOLOGICAL ACTIVITY AND TOXICITY (PART I)
Tuesday, May 22nd
0RGHUDWRUV1DWDOLMD$WDQDVRYD3DQFHYVND)LOLS9DUJD
3OHQDU\/HFWXUH-RKDQQHV1RYDN
Chemistry and Bioactivity of 6DOYLDRI¿FLQDOLV L. and Related Species – a 75
Multi-Layered Picture
,QYLWHG/HFWXUH+DUWZLJ6FKXOW]
9:35 Analysis of Aromatic and Medicinal Plants Applying Various Vibrational 76
Spectroscopy Techniques – Possibilities and Limitations
1DWDOLMD$WDQDVRYD3DQFHYVND']RNR.XQJXORYVNL
,Q9LWUR Antifungal Potency of Extract from Anise Fruits (3LPSLQHOODDQLVXP 77
L.) and Five Essential Oils Against Some Plant Pathogenic Fungi
Alev Önder6HUDS*XU'LGHP<LOPD]2UDO
The Clove Essential Oil and Its Major Component Eugenol Successfully
78
Restored Erectile Dysfunction of Streptozotocin-Induced Type I Diabetes in
Rats
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5DGX,RQLWD3DXOD$OH[DQGUD3RVWX0DULXV0LKDVDQ'UDJRV/XFLDQ*RUJDQ
Cornelia Mircea, 0RQLFD+DQFLDQX/XFLDQ+ULWFX2DQD&LRDQFD
Ameliorative Effects of 0DWULFDULDFKDPRPLOOD L. Hydroalcoholic Extract 79
on Scopolamine-Induced Memory Impairment in Rats: A Behavioral and
Molecular Study
1DWDOLMD$WDQDVRYD3DQFHYVND']RNR.XQJXORYVNL
Antibacterial Activity of Crude Extracts of 3LQXVQLJUD J.F. Arnold and 3LQXV 80
V\OYHVWULV L. Compare with Alpha- and Beta- Pinene
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81
Botanicals in Food Supplements and Novel Food
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82
Phytochemical Evaluation of ,Q9LWUR Cultures from $VWUDJDOXVJO\F\SK\OORV
L.
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Biogenetic Relationship between Some,QXOD Species Growing in Bulgaria 83
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Antimicrobial and Antifungal Activities of Methanol Extract of $UJHPRQH 84
PH[LFDQD L. and Its Anti-Hepatitis Potential
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+RZ'RHV$ELRWLF6WUHVV,QÀXHQFHD+HDOWK3URPRWLQJ3K\WRFKHPLFDOVLQ 85
%UDVVLFDCrops?
Salim Bouchentouf
Molecular Modeling and Molecular Docking for Medicinal Plant 86
Valorization
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&DUPHQ(ğHEUHQFXElvira Gille2DQD7&LXSHUFD5DGX1HFXOD5X[DQGUD0
Cretu, Ionescu Elena
87
Evaluation of the Polyphenolic and Phytosterolic Content from 6FLUSRLGHV
KRORVFKRHQXV(L.) Soják
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0 88
Bioactive Compounds in %UDVVLFDROHUDFHD L. Vegetables Traditionaly Grown
in Croatia
Rumyana Simeonova, Vessela Vitcheva, Magdalena Kondeva-Burdina, Dimitrina
Zheleva, Vessela Balabanova, Reneta Gevrenova
89
Protective Potential of Lyophilized Extract of &OLQRSRGLXPYXOJDUH L.
(Lamiaceae) in Spontaneously Hypertensive Rats (SHRS)
=ODWLQD.RNDQRYD1HGLDONRYD3DUDVNHY1HGLDONRY
8+3/&+506%DVHG)ODYRQRLG3UR¿OLQJRIWKH$HULDO3DUWVIURP 90
&KHQRSRGLXPIROLRVXP Asch. (Amaranthaceae)
Elvira Gille, Ruxandra Mihaela Cretu, Valentin Grigoras, Radu Necula, Dana
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91
Phytochemical Evaluation of Some Food Supplements with 0HOLVVD
RI¿FLQDOLV L. from Romania
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Sponsor Section

Gala Dinner
Poster Section Ph-1 (Tuesday, May 22nd, 14:15-15:15)

P-35 Nina Djapic9MHNRVODY6DMIHUW
Volatile Compounds from )RHQLFXOXPYXOJDUH Mill. Yellow Leaves Obtained by 92
Supercritical Fluid Extraction
P-36 $GDP0DWNRZVNL6RQLD3DQGHO(ZD0XUD6HEDVWLDQ*UDQLFD$QDWROL\'RURVKHQNR
1RQQD,O\LQVND7DWLDQD*RQWRYD<DQD.ULXNRYD
93
Polyphenol Diversity and ,Q9LWUR Antioxidant Properties of Tubers of Ornamental
'DKOLD Varieties
P-37 Majid Jafarimoghadam, Elham Ghasemi, Nasim Khalesi
The Effects of Organic Fertilizers on Carotenoid Compounds and Yield in Saffron 94
(&URFXVVDWLYXV L.)
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Antibacterial Activity of Different Leaf Extracts of &HQWDXUHDFDOFLWDUSD L. from Serbia
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96
2ULJDQXPPDMRUDQD L. and 2YXOJDUH L. from Serbia are Promising as Functional
Food
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Antioxidant and Antineurodegenerative Activities of 0HOLVVDRI¿FLQDOLV L. And 0HQWKD 97
X SLSHULWD L. Cultivated in Serbia
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Phytotoxic Activity of Aqueous Extracts from Different $UWHPLVLDDEVLQWKLXP L. 98
Chemotypes on Seed Germination of Lettuce and Basil
P-42 Maja Molnar, 0DUWLQD-DNRYOMHYLü6WHOD-RNLü
Optimization of Supercritical Fluid Extraction of Carnosic Acid from Sage Leaves 99
(6DOYLDRI¿FLQDOLV L.)
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100
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Combination of Cytostatic and Antioxidant Principles in Leukemia Cells Treatment
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Standardized $MXJDJHQHYHQVLV L. Aerial Parts Extracts with Antitumor Potential
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102
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Effect of Inoculation with Salt Tolerant Strain of 5KL]RELXP on Growth and Protein 103
Production of Alfalfa under Salt Constraints
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Ionic Liquid-Supported Solid–Liquid Extraction for the Quantitative Determination of 104
Galantamine in /HXFRMXPDHVWLYXP L. (Amaryllidaceae)
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Phytochemical Evaluation and Assessment of Biological Effects of $MXJD5HSWDQV L.
Aerial Parts from Romania
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Short Review of the Anticancer and Cytotoxic Activity of Some Species from Genus 106
(XSKRUELD
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Ionic Liquid-Assisted Micellar Extraction for the Quantitative Determination of 107
Sesquiterpenic Acids in 9DOHULDQDRI¿FLQDOLV L. (Caprifoliaceae)
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Microstructure of the Common Selfheal (3UXQHOODYXOJDULV L.) Nutlets and Mucilage 108
Envelope
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Effect of $UWHPLVLDDEVLQWKLXP L. Extract against )DVFLRODKHSDWLFDLarvae
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9HURQLFDF\PEDODULD Bodard – When the GC-MS Peaks of Volatile Compounds Appear
P-55 <XOLDQ 9R\QLNRY 3DUDVNHY 1HG\DONRY 'LPLWULQD =KHOHYD'LPLWURYD 9HVVHOD
Balabanova, Reneta Gevrenova
112
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Phytochemical Analysis and Antiradical Potential of Methanol Extracts of +LHUDFLXP 113
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Antioxidant, Cytotoxic, Acetylcholinesterase and Tyrosinase Enzyme Inhibitory
Activities of Six ,QXOD Species from Bulgaria
P-58 Blagica Jovanova6YHWODQD.XOHYDQRYD7DWMDQD.DGLINRYD3DQRYVND
Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of 115
Selected Aromatic Herbs
P-59$QGUH\60DUFKHY'DULQD7<D]RYD,YDQND..R\FKHYD, Madeleine Neumann, Jutta
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116
NMR-Based Metabolomics of 9HUEDVFXPSXUSXUHXP (Janka) Hub. - Mor. and Their
Transformed Roots
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117
Antioxidative Activity of Leaf Extracts of $FKLOOHDFRDUFWDWD Poir. and +LHUDFLXP
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Antioxidative Activity of Leaf Extracts of Three $UWHPLVLD Species from SE Europe
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Determination of Total Hypericins of +\SHU\FXPSHUIRUDWXP L., from Different
Locations in Western R. Macedonia
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Evaluation of Inhibitory Potential of /LJXVWLFXPDODWXP (Bieb.) Sprengel Essential Oils
and Extracts against Enzymes Involved in Alzheimer Disease and Type II Diabetes
P-64 Stefan Kolašinac,OLQND3HüLQDU6WHYD/HYLü'UDJDQD5DQþLü=RUD'DMLü6WHYDQRYLü
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Raman Spectroscopic Characterization of Carotenoids from Rose Hips Herbal Tea
Mixtures
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0LFURPHULDFURDWLFD (Pers.) Schott - Chemical Composition and Variability of Essential
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,QÀXHQFHRI5KRGLRODURVHD L. Calli Extracts on CD4 T Cells Phenotype Changes
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HPLC-DAD-ESI-MSn Analysis of Phenolic Compounds in +\SHULFXPSHUIRUDWXP L.
Wild-Growing Plants from Pelister National Park, Republic of Macedonia
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Production of Nitric Oxide, Reactive Oxygen Species and COX2 by Neutrophils under
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Chemical Composition and Free Radical Scavenging Properties of 3XOLFDULDVDOYLLIROLD 127
Bunge., 3JQDSKDORGHV (Vent.) Boiss. and 3XOLJLQRVD Steven Ex Dc. Essential Oils
P-70 Marin Zagnat, Corina Cheptea
Comparative Analysis of Volatile Oils Obtained from Seven Romanian Brands of 128
Chamomile Tea
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Phenolic Compounds in Selected Plant Species
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Glycyrrhizin Content in the Roots of *O\F\UUKL]DJODEUD L. from Bulgarian Populations
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Antioxidant Capacity and Total Polyphenol Content of Lavender Cultivars (L. 132
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Variability of Total Essential Oils Content in Leaves of Bay Laurel (/DXUXVQRELOLV L.) in 133
12 Distinct Populations of Croatian Adriatic Area in Relation with Geographic Position
of Locations, Leaf Lamina Surface and Genetic Variability
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Antidepressant-Like Effect of Salidroside and Curcumin on the Immunoreactivity of
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Antioxidant System during Micropropagation?
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Cavernosum Tissue
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Block Copolymer Nanocarriers of Caffeic Acid Phenethyl Ester: Preparation and
Antioxidant Activity
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Chemical Characterization and Radical-Scavenging Activity of Propolis from Pitcairn
Island
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of Nine +HUDFOHXP Taxa from Southeastern Europe
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Screening of Cytotoxicity of &HQWDXUHD Species Aqueous Extracts Fractions on Human
Fibroblasts
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Endemic Plants
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Pulmonariosides A and B – New Phenolic Compounds from the Aerial Part of 3XOPRQDULD 145
RI¿FLQDOLV L.
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Danova, Marina Dimitrova, Iva Doycheva, Ivan Evtimov, Katya Georgieva, Kristina
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146
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Genus 7K\PXV in Bulgaria – a New Project Aimed at Revealing of Species’ Metabolite
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BIOLOGICAL ACTIVITY AND TOXICITY (PART II)
Thursday, May 24th
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148
Novel Extraction Technologies for Medical and Aromatic Plants
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,RDQ7RPXWD/DXULDQ9ODVHDaniela-Saveta Popa
9:25 149
The Investigation of Biological Activity of Several Walnut (-XJODQVUHJLD L.)
Septum Extracts
6KNHOTLP.DUDM$OLFH-DTXHOLQH+DFN-RDFKLP0OOHU
Thin Layer Drying Behaviour of IROLXPFXPÀRUH&UDWDHJL from Different 150
Plant Flowering Stages
Reneta Gevrenova%DUEDUD.RáRG]LHM0D[+HQU\
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9:55 151
Oleanane-Type Triterpenoid Carboxylic Acid 3, 28-O-Bidesmosides
(Gotcab) Saponins. A Case Study of *\SVRSKLOD L. (Caryophyllaceae)
9HVVHOD %DODEDQRYD 1LNR %HQEDVVDW <XOLDQ 9R\QLNRY 5HQHWD *HYUHQRYD
Dimitrina Zheleva-Dimitrova
152
State of the Art Method on the Base of GC-Orbitrap-HRMS for
Sesquiterpene Lactones in $UQLFDPRQWDQD L
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+ULVWRY3DXOLQD6XJDUHYD9DOHQWLQ/R]DQRY
153
Bioinformatic Inside on 3RUWXODFD ROHUDFHD L. (Purslane) with Bulgarian
and Greek Origin
Marius Emil Rusu, Andrei Mocan, Ana-Maria Gheldiu, Daniela-Saveta Popa,
,RDQ7RPX‫܊‬D/DXULDQ9ODVH
154
Optimal Experimental Design Developed to Prepare Walnut (-XJODQVUHJLD
L.) Septum Extracts with High Content of Bioactive Compounds
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Essential Oil Composition of 7DQDFHWXP3DUWKHQLXP L. from Eastern Black 155
Sea Region, Turkey
Cornelia Mircea &ULVWLQD 7XFKLOXV 2DQD &LRDQFD &ULVWLQD ,DQFX 8UVXOD
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Common Juniper (-XQLSHUXVFRPPXQLV L.) in Slovakia
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Characterisation of Medicinal and Aromatic Plants (MAPS)
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P-88 /H9LHW'XQJ/H9DQ0LQK
An ,Q 9LWUR Study on &RPEUHWXP TXDGUDQJXODUH Kuz., 9HUQRQLD DP\JGDOLQD Del.
159
and &OHURGHQGUXP LQHUPH (L.) Gaertn. Plant Species from the South of Vietnam for
Į-Amylase Inhibitory Activity
P-89 Gabriela Vochita, Cosmin-Teodor Mihai, Pincu Rotinberg, Elena Ionescu, Carmen-
Elena Tebrencu, Ruxandra-Mihaela Cretu, Daniela Gherghel
160
Preliminary Data on the Impact of 6RUEXVDXFXSDULD L. Bark Extract on ,Q9LWUR Cell
Viability
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1LQD&LRFDUODQ;DYLHU6LPRQQHW 161
3KHQROLF&RQWHQWDQG3UR¿OHRI7KUHH$UWHPLVLD Species from Republic of Moldova
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Variability and Composition of Essential Oil Content of Selected Spices (2FLPXP 162
EDVLOOLFXP L., 6DWXUHMDKRUWHQVLV L. and 0DMRUDQDKRUWHQVLVMoench.)
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Main Phenolic Compounds and Antioxidant Activity of Methanolic Extract of &DUGXXV 163
NHUQHUL Simonkai ssp. $XVWUR2ULHQWDOLV)UDQFR,QÀRUHVFHQFHV
P-93 2DQD &LRDQFD, Cristina Cerasela Mircea (Arsene), Ana Flavia Burlec, Adrian Spac,
$QGUHHD&RUFLRYD(OYLUD*LOOH6LOYLD5REX0RQLFD+DQFLDQX
164
0RUSKRORJLF DQG &KHPLFDO 3UR¿OH RI 7ZR $MXJD Species Cultivated in Northern
Romania
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Content of Biologically Active Compounds in +\VVRSXV RI¿FLQDOLV L. Traditionally 165
Cultivated, In Vitro Propagated and Plants from Natural Habitats
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6DIQHU'DOLERU%HGHNRYLü 166
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Concern on the Pharmacopoeia Extraction Procedure for Determination of Arbutin 167
Content in Bearberry Leaves
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Cristina Iancu, Ana Flavia Burlec, Andreea Corciova, Ivan Salamon, 0RQLFD+ăQFLDQX
168
Investigation of the Antibacterial Activity of Some Anthocyanidin-Rich Extracts on
Clinical Isolates of UPEC
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'UDJRYLü8]HODF
169
Microwave and Ultrasound Assisted Extractions of Polyphenols from Dandelion
(7DUD[DFXPRI¿FLQDOH (L.) Weber Ex F.H. Wigg) Root and Leaf
P-99 Magdalena Kondeva-Burdina, Georgi Popov, $OHNVDQGDU 6KNRQGURY, Ilina Krasteva,
Vassil Manov
170
Evaluation of the Antioxidant Potential of Alcesefoliside from $VWUDJDOXVPRQVSHVVXODQXV
L. in ,Q9LWUR Cell Systems
P-100 Valentin Grigoras 5X[DQGUD0LKDHOD &UHWX 5DGX 1HFXOD *HRUJLDQD/XPLQLWD
Gavril, Elvira Gille 171
Characterization of Invazive Plant Species for Possible Use in Phytoterapy
P-101 7HUH]D0XFKRYi-DUPLOD1HXJHEDXHURYi
172
Evaluation of the Edible Flowers of Medicinal Plants
P-102'LDQGUD3LQWDü0DULMD/HVMDN1DWDãD6LPLQ'HMDQ2UþLü1HGD0LPLFD'XNLü
Comparison of Phenolic Composition and Biological Activity of Commercially Available 173
White Wines Originating from Fruška Gora, Serbia
P-103 18NZXDQL.ZDMD7$2PRGHOH68PDU
Antinociceptive Activity of Methanolic Leaves Extract of &UDWHYD$GDQVRQ Dc. in Albino 174
Rat
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Extraction of Pyrethrins from Dalmatian Pyrethrum (7DQDFHWXP FLQHUDULLIROLXP / 175
Trevir. / Sch. Bip.) By Matrix-Solid Phase Dispersion
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176
Evaluation of *O\F\UUKL]D L. Genetic Resources in the Czech Republic
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177
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Activities of Endemic -XULQHDW]DUIHUGLQDQGLi Davidov
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Chitosan-Pectine Microparticles Loaded with Sage Extract for Extending Shell-Life of
Sausages
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Elvira Gille 179
Nutraceuticals Based on Bioactive Principles of Vegetal Origin
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Carrot Juice as Functional Food of Plant Origin
P-1101DGLD)HNLK+RFLQH$OODOL6DOLPD0HUJKDFKHSalim Bouchentouf, Jean Costa
,Q9LWURAssessment of Two Species of the Genus 3LQXVGrowing in Algeria for Their 181
Antimicrobial and Antioxidant Activity
P-111 ,YRQD (OH] *DURIXOLü 6DQGUD 3HGLVLü 6DQMD 'UDJRYLü 0DWHMD &HVWDU 9DOHULMD
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Activity of Mastic Tree Leaves
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Kulevanova 183
Antioxidative Potential of Macedonian Pine
P-113 Cristina Iancu, Cornelia Mircea 2DQD &LRDQFD $QGUHHD 0XUăUD‫܈‬X 1LQD )LOLS
0DGDOLQD0RFDQX0RQLFD+DQFLDQX
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Activity
P-114 $]UDĈXORYLü$QGUHD6HGODU9HGUDQDýLNHãýXOLü,YDQD*HQHUDOLü0HNLQLü)UDQNR
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%XQLDVHUXFDJR L.: Chemistry and Biological Activity
III. CULTIVATION, BREEDING AND BIOTECHNOLOGY
OF MEDICINAL AND AROMATIC PLANTS
Thursday, May 24th
0RGHUDWRUV)UDQN0DUWKH%RULV/D]DUHYLü
3OHQDU\/HFWXUH±0LOHQ,*HRUJLHY
Metabolomics, Plant Biotechnology and Lead Finding: Perfect Holistic 187
Match?
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Choosing the Right Approach for Conducting Medicinal Plants Cultivation 188
Trial
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189
Effect of Irrigation on the Production and Secondary Metabolites of Summer
Savory (6DWXUHMDKRUWHQVLV L. ‘Budakalászi’)
)XUNDQ&REDQ+DNDQ2]HU
Impact of Nitrogen Fertilization and Sowing Norms on Chlorophyll 190
Fluorescence of Fenugreek (7ULJRQHOODIRHQXPJUDFHXP L.) Genotypes
Ivan Salamon
191
The Successful Breeding Work of Medicinal Plants in Slovakia
6WHIIHQ6FKRFN, Sebastian Romuli, Joachim Müller
Drying Performance of Amaranth Leaves ($PDUDQWKXV Spp) using an 192
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2DQD &LRDQFD, Cristina Cerasela Mircea (Arsene), Ana Flavia Burlec, Adrian
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Metabolites in Fertilized Ornamental Asteraceae
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Two New Chemotypes of Balm (0HOLVVDRI¿FLQDOLV L.)
Closing Ceremony
Poster Section C-1 (Thursday, May 24th, 13:40-14:40)

P-115 9ODGLPLU,OLQNLQ, Marina Stanilova
Initiation of ,Q9LWUR Clones of 7DQDFHWXPFLQHUDULLIROLXP Trevir. Sch. Bip. 195
(Asteraceae)
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Growth and Essential Oils of 6DOYLDRI¿FLQDOLV L. Plants Derived from Conventional 196
or Aeroponic Produced Seedlings
P-117 %RU\DQND7UD\NRYD, Marina Stanilova
Rooting of Leaves of +DEHUOHDUKRGRSHQVLV Friv. (Gesneriaceae) using a Vertical 197
Aeroponic System
P-118 /LO\DQD1DFKHYD, Nataliya Dimitrova, Valeria Ivanova
,Q9LWUR Establishment and Culture of Endangered Medicinal Plants with 198
Ornamental Value &DPSWRWKHFDDFXPLQDWD Decne (Nyssaceae)
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Antioxidant Enzymes and Oxidative Stress Markers in +\SHULFXPSHUIRUDWXPL.
Transgenic Shoot Cultures
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Iatrou, )RWLQL1/DPDUL
Seasonal Variation of the Essential Oil and Polyphenol/Flavonoid Content of Leaves 200
of 6DOYLDIUXWLFRVD Mill. And 6SRPLIHUD L. Plants Cultivated in the Botanical
Garden of University of Patras
P-121 Ely Zayova0DULD*HQHYD/XGPLOD'LPLWURYD0DULD3HWURYD
201
Shoot Multiplication System of Hyssop (+\VVRSXVRI¿FLQDOLV L.)
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Gianoulis, 3DVFKDOLQD&KDW]RSRXORX
202
The Effect of Irrigation and Organic Fertilization on the Essential Oil Composition
of 0DWULFDULDFKDPRPLOOD L.
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Abraham
203
Phenotypic Evaluation of 6LGHULWLVVFDUGLFD Gris. Selected Clones, and Pre-Breeding
Evaluation for Yield Potential and Qualitative Traits
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Phytochemical Investigations of Three Different Accessions of 3HULOODIUXWHVFHQs (L.) 204
Britt
P-125 $QWRQtQ9DFXOtN+HOHQD3OXKiþNRYi-DQD%HQHãRYi
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Options of Herbicides Protection of Marian Thistle (6LO\EXPPDULDQXP L.)
P-126 Smiljana Goreta Ban, *YR]GHQ'XPLþLü
206
Auxin Application to Enhance Rooting of Dalmatian Sage, Immortelle and Lavandin
P-127 'DQLHO%HFNHU)UDQN0DUWKH
207
Breeding Synthetics in Caraway (&DUXP&DUYL L.)
P-128 *6WHINRY=.HVNRYVNL0'DUNRYVND6HUD¿PRYVND
Cultivation Practices of the First Large Scale Indoor Facilities for Production of High 208
THC Cannabis in Republic of Macedonia
Book of abstracts – 10th CMAPSEEC
I. MEDICINAL AND AROMATIC PLANTS ECOLOGY,

DIVERSITY AND ETHNOBOTANY
24 May 20-24, 2018, Split, Croatia

Book of abstracts – 10th CMAPSEEC Plenary lecture
ETHNOBOTANY OF THE ADRIATIC ISLANDS IN

CROATIA
àXNDV]àXF]DM1,YDQD9LWDVRYLü.RVLü20DULMD-XJ'XMDNRYLü3, Katija
Dolina40LUMDQD-HULþHYLü5

'HSDUWPHQWRI%RWDQ\)DFXOW\RI%LRORJ\8QLYHUVLW\RI5]HV]yZXO=HOZHURZLF]D%
5]HV]yZ3RODQG
2
Department of Agricultural Botany, Faculty of Agriculture, University of Zagreb,
6YHWRãLPXQVNDFHVWD=DJUHE&URDWLD
3
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5
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DUHLQKDELWHG7KH$GULDWLFLVODQGVDUHYHU\DWWUDFWLYHSODFHVIRUWRXULVP7KH\DUHLQÀXHQFHG
ERWK E\ WKH LQÀX[ RI ZHOORII QHZ LQKDELWDQWV IURP GLIIHUHQW FRXQWULHV RI (XURSH DQG WKH
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QXPHURXVZLOGYHJHWDEOHVDQGDUHZLOOLQJWRWDONDERXWLW,QFRQWUDVWWRWKLVVRPHODUJHLVODQGV
KDYH DOPRVW FRPSOHWHO\ ORVW WKHLU NQRZOHGJH RI SODQWV7KLV LV IRU H[DPSOH WKH FDVH ZLWK
'XJL2WRNDQGWKH/DVWRYRLVODQGV2XUSUHOLPLQDU\UHVXOWVVXJJHVWWKDWNQRZOHGJHZDVEHWWHU
preserved on larger islands with living agriculture and with more lively communities, where
WKHLQWHUFKDQJHDQGWUDQVIHURINQRZOHGJHLVHDVLHU2QVPDOOHULVODQGVWKHUHDUHIHZHUKDELWDWV
for gathering wild edible plants, and a large proportion of inhabitants are retired people who
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OLIH$SDUWIURPRQ.RUþXODVHYHUDOLQGLYLGXDOVZLWKFRQVLGHUDEOHUHVHUYRLUVRINQRZOHGJHFDQ
VWLOOEHIRXQGRQ%UDþâROWDDQG3DãPDQ
Key words: ethnobotany, wild vegetables, wild edible plants, wild food plants
May 20-24, 2018, Split, Croatia 25

Book of abstracts – 10th CMAPSEEC Oral presentations – Invited speaker
CHLOROPLAST DNA DIVERSITY AND

PHYLOGEOGRAPHY OF SALVIA OFFICINALIS L. AND
SALVIA LAVANDULIFOLIA Vahl
Zlatko Liber1,2'DQLMHOâNUWLü1,YDQ5DGRVDYOMHYLü1,2, Marija Jug-
'XMDNRYLü3, Llorenç Sáez4, Romeo di Pietro5=ODWNRâDWRYLü2,6

University of Zagreb, Faculty of Science, Division of Biology, Department of Botany and
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2
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3
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6SOLW&URDWLD

Universitat Autònoma de Barcelona, Facultat de Biociències, Carrer de la Vall Moronta,
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5
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University of Zagreb Faculty of Agriculture, Department of Seed Science and Technology,
6YHWRãLPXQVNDFHVWD+5=DJUHE&URDWLD
Dalmatian sage (6DOYLD RI¿FLQDOLV / DQG 6SDQLVK VDJH Salvia lavandulifolia Vahl) are
perennial, evergreen shrubs from the family Lamiaceae %HFDXVH RI WKH HVVHQWLDO RLOV ERWK
VSHFLHVKDYHEHHQWUHDVXUHGDVPHGLFLQDOKHUEVThe taxonomic position of S. lavandulifolia
is still controversial since certain authors consider it as subspecies of the 6RI¿FLQDOLVThe
natural distribution area of 6RI¿FLQDOLVLVFRDVWDOUHJLRQRIWKHZHVWHUQ%DONDQDQGVRXWKHUQ
Apennine Peninsulas while S. lavandulifoliaLVQDWLYHWR,EHULDQ3HQLQVXOD*enetic diversity
and phylogeographical pattern of Dalmatian and Spanish sage were analysed comparing two
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SRSXODWLRQVTwelveFKORURSODVWKDSORW\SHVZHUHIRXQGRQWKH%DONDQ3HQLQVXOD6RI¿FLQDOLV),
six on the Apennine Peninsula (6RI¿FLQDOLVDQGRQ,EHULDQ3HQLQVXODS. lavandulifolia
7KH WZR VSHFLHV GLG QRW VKDUH KDSORW\SHV ZKLOH %DONDQ DQG $SHQQLQH SRSXODWLRQV RI S.
RI¿FLQDOLV KDG WZR KDSORW\SHV LQ FRPPRQ 6 RI¿FLQDOLV exhibited higher haplotype and
nucleotide diversity than S. lavandulifolia
Key words: 6DOYLD RI¿FLQDOLV, Salvia lavandulifolia, phylogeography, chloroplast DNA,

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WILD RASPBERRY FROM THE CENTRAL BALKAN:

FROM ETHNOBOTANY TO BIOACTIVITY
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=RUD'DMLü6WHYDQRYLü3

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2
Department of Biology and Ecology, Faculty of Science, University of Kragujevac 3Faculty
of Agriculture, University of Belgrade
Raspberry (Rubus idaeus/5RVDFHDHLVDZHOONQRZQVSHFLHVQDWLYHWR(XURSHDQGQRUWK

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leaves, fruits and some traditional fruit products of wild raspberry populations of the central
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7KHYDOXHVREWDLQHGIRUWRWDOSKHQROVUDQJHGIURPWRPJRI*$JDQGWR
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ZDV GHWHFWHG LQ WKH OHDI PHWKDQRO H[WUDFWV WKDQ LQ WKH IUXLW H[WUDFWV /HDI DQG IUXLW H[WUDFW
were the most effective against Escherichia coli$7&&$QWLFDQFHUDFWLYLW\ZDVVWXGLHG
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performed at the nine high-mountain rural sites (corresponding to sites where plant material for
phytochemical study was collected) belonging to regions of southwest (SWS) and southeast
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mostly used for prevention and healing of gynecological disorders, and during pregnancy and
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raspberry traditional products can be recommended as healthy, or even the functional food, but
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Key words: Rubus idaeus, phenols, antioxidant, antimicrobial, anticancer, leaf, fruit,
traditional products

Book of abstracts – 10th CMAPSEEC Oral presentations
WILD LABIATES AROMATIC PLANTS AND THEIR BEE

POLLINATORS
Ekaterina Kozuharova1, Ina Aneva2, Hristo Valchev3, Dave Goulson4

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Bulgaria
2
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3
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Particularly important is the pollination process when seed reproduction is the leading way
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EHHQREVHUYHGRYHUUHFHQWGHFDGHV7KHDLPRIWKLVUHYLHZVWXG\LVWRHYDOXDWHWKHLPSRUWDQFH
RIEHHVDVSROOLQDWRUVRIZLOG/DPLDFHDDURPDWLFSODQWV7KHREMHFWRIRXUVWXG\ZHUHAcinos
arvensis/DP'DQG\A. suaveolens6HW6*'RQCalamintha nepeta/6DYLNepeta
cataria / Origanum vulgare / Mentha VS GLY Thymus VS GLY Micromeria dalmatica
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Key words:KHUEDOWHDDURPDWLFEHHV/DPLDFHDH

THE USE OF MEDICINAL PLANTS IN THE KNIN AREA,

CROATIA
0DULMD-XJ'XMDNRYLü1,YDQDâROLü2, Martina Grdiša2,4, Lukasz Luczaj3,
Filip Varga2,4

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Croatia
2
University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology,
3
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.QLQDUHD)RUW\WZRQDWLYHLQKDELWDQWVIHPDOHDQGPDOHZHUHLQWHUYLHZHGLQVHPL
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IDPLOLHVZHUHUHFRUGHG5RVDFHDHZDVWKHPRVWUHSUHVHQWHGIDPLO\ZLWK species followed by
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followed by Plantago major/DQGRumex pulcher/ZKLOHVSHFLHVZHUH
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0LOOHelichrysum italicum5RWK*'RQDQGRumex pulcher/DQG)/RIZDVVFRUHGE\
Equisetum arvense/Sempervivum tectorum/DQGCrataegus monogyna-DFT7KHGLVHDVHV
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recorded plant species were for treating digestive system disorders VNLQVXEFXWDQHRXV
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Key words: ethnobotany, PHGLFLQDOSODQWVWUDGLWLRQDONQRZOHGJH

ENVIRONMENTAL POLLUTION AND HEAVY METALS

ACCUMULATION IN SALIX ALBA L. (SALICACEAE),
ALONG THE RIVER STREAM OF SITNICA
Demush Bajraktari1, Biljana Bauer2, Zoran Kavrakovski2, Lulzim
Zeneli3

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Sitnica, which passes near the Kosovo Thermal Power Plants, and accumulation of selected
KHDY\PHWDOV$V&G&U1LDQG3ELQZDWHUVRLODQGZLOORZEDUNVDPSOHVSalix alba/
Salicaceae), considering the fact that industrial area of Kosovo Thermal Power Plants is one
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that the content of these pollutants in this study area, differ within elements and samples, and
they indicate a possible threat to environment pollution and impact of the industrial sector in
WKHOLYLQJHQYLURQPHQW&KURPLXPQLFNHODQGOHDGLQZDWHUVDPSOHVZHUHDERYHWDUJHWYDOXHV
DQG LQ VRPH FDVHV DERYH LQWHUYHQWLRQ YDOXHV +HDY\ PHWDOV FRQWHQW LQ VRLO VDPSOHV YDULHG
:KLOHFKURPLXPZDVZLWKLQSHUPLVVLEOHOLPLWVPD[FRQFHQWUDWLRQVRIDUVHQLFFDGPLXPDQG
OHDG ZHUH DERYH LQWHUYHQWLRQ YDOXHV ,Q ZLOORZ EDUN VDPSOHV FRQFHQWUDWLRQV RI QLFNHO ZDV
GHWHFWHGLQWKHXSSHUDOORZHGOLPLWV&DGPLXPDQGFKURPLXPPRVWO\ZHUHDERYHSHUPLVVLEOH
OLPLWV5HVXOWVLQGLFDWHGDSRVVLEOHWKUHDWWRHQYLURQPHQWSROOXWLRQDQGLPSDFWRIWKHLQGXVWULDO
VHFWRULQWKHHQYLURQPHQWDQGLIDGHTXDWHSURWHFWLRQPHDVXUHVDUHQRWWDNHQGHJUDGDWLRQRI
OLYLQJHQYLURQPHQWFDQFRQWLQXH
Key words: heavy metals, analysis, accumulation, environmental pollution

STRONG INFLUENCE OF CLONALITY ON THE

FINE SCALE SPATIAL AND GENETIC STRUCTURE
OF SALVIA BRACHYODON VANDAS (LAMIACEAE)
POPULATION
,YDQ5DGRVDYOMHYLü1,2=ODWNRâDWRYLü2,32OHJ$QWRQLü47RQL1LNROLü1,
Dario Hruševar1, Zlatko Liber1,2

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Salvia brachyodon 9DQGDV LV D QDUURZO\ HQGHPLF VSHFLHV UHVWULFWHG WR RQO\ WKUHH NQRZQ
populations in the central Adriatic region and it is treated as a highly vulnerable species in
0RQWHQHJURDQGVWDWXWRULO\VWULFWO\SURWHFWHGLQGLJHQRXVSODQWVSHFLHVLQ&URDWLD,WLVDSDUWLDOO\
clonal species and reproduces both sexually through seeding and clonally from perennial
XQGHUJURXQGVWRORQV7KHVSHFLHVLVFKDUDFWHUL]HGE\ULFKFRQWHQWRIHVVHQWLDORLORIZKLFK
chemical composition is rather similar to other closely related sage species including well-
VWXGLHGFRPPRQVDJH7KHVWXGLHGSRSXODWLRQRIS. brachyodon, located on Pelješac Peninsula,
&URDWLDLVWKHODUJHVWRIDOONQRZQSRSXODWLRQV,QRUGHUWRDVVHVVOHYHOVRIJHQHWLFGLYHUVLW\
LQGLYLGXDOV ZHUH JHRUHIHUHQFHG VDPSOHG DQG JHQRW\SHG XVLQJ HLJKW PLFURVDWHOOLWHPDUNHUV
For additional assessment of trade-off between sexual and clonal reproduction, the ramets
DQGLQÀRUHVFHQFHZHUHFRXQWHG3RSXODWLRQJHQHWLFDQDO\VLVUHYHDOHGKLJKOHYHOVRIJHQHWLF
GLYHUVLW\ZLWKQRVLJQL¿FDQWGHSDUWXUHIURP+DUG\:HLQEHUJHTXLOLEULXP$OWKRXJKQXPHURXV
small clones dominated population over only few large ones, clonal reproduction strongly
SUHYDLORYHUVH[XDOUHSURGXFWLRQ6LQFHGLIIHUHQWJHQHWVYLUWXDOO\GLGQRWintermingle with each
RWKHUSRSXODWLRQ¶VFORQDODUFKLWHFWXUHUHOLHVRQSKDODQ[VWUDWHJ\RIUDPHWV¶VSDWLDOGLVWULEXWLRQ
5HVXOWVUHYHDOHGWKDWFORQHVRIGLIIHUHQWVL]HVÀRZHUZLWKVLPLODULQWHQVLW\DQGFRQVHTXHQWO\
WKHODUJHUFORQHVFRQWULEXWHPRUHWRVH[XDOUHSURGXFWLRQWKDQWKHVPDOOHURQHV7KHDQDO\VLV
RI FORQH VL]H GLVWULEXWLRQ UHYHDOHG WKDW VWXGLHG SRSXODWLRQ ZDV FKDUDFWHUL]HG E\ 5HSHDWHG
Seedling Recruitment (RSR) strategy of new genets recruitment, which assumed constant
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ODUJHDQGVPDOOFORQHVLPSO\LQJVSHFL¿FFRORQL]DWLRQVWUDWHJ\RIWKHSRSXODWLRQ
Key words: Salvia brachyodon Vandas, clonality, genetic diversity, genets, ramets

MICROSATELLITE DIVERSITY OF DALMATIAN SAGE

POPULATIONS FROM THE BALKAN AND APENNINE
PENINSULA
=ODWNRâDWRYLü1,2'DQLMHOâNUWLü3,YDQ5DGRVDYOMHYLü2,3, Marija Jug-
'XMDNRYLü4, Romeo di Pietro5, Zlatko Liber2,3

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GHOO¶$UFKLWHWWXUD9LD)ODPLQLD5RPH,WDO\
Dalmatian sage (6DOYLD RI¿FLQDOLV / /DPLDFHDH LV D ZHOONQRZQ DURPDWLF DQG PHGLFLQDO
0HGLWHUUDQHDQ SODQW WKDW LV QDWLYH LQ FRDVWDO UHJLRQV RI WKH ZHVWHUQ %DONDQ DQG FHQWUDO DQG
VRXWKHUQ $SHQQLQH 3HQLQVXODV DQG LV FRPPRQO\ FXOWLYDWHG ZRUOGZLGH :H XVHG HLJKW
PLFURVDWHOOLWHPDUNHUVWRLQYHVWLJDWHJHQHWLFGLYHUVLW\DQGSRSXODWLRQVWUXFWXUH$WRWDORI
VSHFLPHQVIURPORFDWLRQVDFURVVWKH%DONDQ3HQLQVXODDVZHOODVIURPORFDWLRQVIRUPWKH
$SHQQLQH3HQLQVXODZHUHVDPSOHGDQGJHQRW\SHG7KHPRGHOEDVHGVWUXFWXUHDQDO\VLVUHYHDOHG
WKHSUHVHQFHRI¿YHJHRJUDSKLFDOO\FRKHUHQWJHQHWLFFOXVWHUVWKDWGLIIHUHGVLJQL¿FDQWO\LQDOOHOLF
ULFKQHVV REVHUYHG DQG H[SHFWHG KHWHUR]\JRVLW\ 7KH KLJKHVW OHYHO RI DOOHOLF ULFKQHVV ZDV
found in populations located in the central part of the eastern Adriatic coast, while decreases in
allelic richness were evident towards the northwestern Adriatic coast and southern and eastern
UHJLRQVRIWKH%DONDQ3HQLQVXOD3RSXODWLRQVIURP$SHQQLQH3HQLQVXODKDGVXEVWDQWLDOO\ORZHU
DOOHOLFULFKQHVVWKDQWKRVHIURPEDONDQ3HQLQVXOD7KHJHQHWLFUHODWLRQVKLSVDPRQJWKHJHQHWLF
FOXVWHUVZHUHHYDOXDWHGEDVHGRQQHWQXFOHRWLGHGLVWDQFH$1HLJKERU±-RLQLQJWUHHEDVHGRQWKH
QHWQXFOHRWLGHGLVWDQFHVZDVJHQHUDWHGVKRZLQJWKDWWKHWZR$SHQQLQHFOXVWHUVZHUHPDUNHGO\
GLYHUJHQWIURPHDFKRWKHUDVZHOODVIURPWKHWKUHH%DONDQFOXVWHUVVXJJHVWLQJWKHLUORQJWHUP
JHQHWLFLVRODWLRQWKDWOHDGWRDUHGXFWLRQLQJHQHGLYHUVLW\
Key words: 6DOYLDRI¿FLQDOLV, medicinal and aromatic plants, plant genetic resources, genetic
diversity, genetic structure

MEDICINAL PLANTS AS A FOOD SOURCE FOR

POLLINATORS – FLUCTUATION OF ATTRACTIVENESS
OF SELECTED SPECIES DURING THE DAY
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WKH LQVHFW SROOLQDWRUV :KHQ HYDOXDWLQJ WKH DWWUDFWLYHQHVV RI LQGLYLGXDO SODQW VSHFLHV LW LV
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QHFWDU DQG SROOHQ LQ D GLIIHUHQW TXDQWLW\ GXULQJ WKH GD\7KLUW\HLJKW VSHFLHV RI 0$3V DV D
VRXUFH RI EXPEOH EHHV JUD]LQJ ZHUH DVVHVVHG RQ H[SHULPHQWDO SORWV [P2 LQ 7KH
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ZLWKIDYRUDEOHZHDWKHUVL[WLPHVSHUGD\±,WZDVIRXQGWKDWVWXGLHGVSHFLHVFDQ
EHFOHDUO\GLYLGHGLQWRVHYHUDOJURXSVDFFRUGLQJWRWLPHRIWKHLUDWWUDFWLYHQHVVIRUEXPEOHEHHV
Although some species (Papaver rhoeas/9HUEDVFXPGHQVLÀRUXP%HUWRO$OWKHDRI¿FLQDOLV
/HWFDUHWKHPRVWDWWUDFWLYHIRUEXPEOHEHHVLQWKHHDUO\PRUQLQJDQGODWHUWKHLUDWWHQGDQFH
GURSV VLJQL¿FDQWO\ IRU PRVW RI WKH HYDOXDWHG 0$3 VSHFLHV WKH SHDN RI EXPEOH EHHV WUDI¿F
LQ WKH ÀRZHUV UHÀHFWHG VRPHZKHUH EHWZHHQ DQG DP 7KH UHVXOWV FOHDUO\ VKRZ
WKDW DVVHVVLQJ WKH VLJQL¿FDQFH RI 0$3V DV ZHOO DV RWKHU SODQWV DV D VRXUFH RI D EXPEOH
bee or alternatively honey bee pasture, cannot be based on observations made in only one
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UHÀHFWVSHFL¿FFKDUDFWHULVWLFVRIQHFWDUDQGRUSROOHQSURGXFWLRQIURPLQGLYLGXDOVSHFLHV7KH
REWDLQHGUHVXOWVPXVWEHWDNHQLQWRDFFRXQWDOVRZKHQGUDZLQJXSWKHYDULRXVVHHGLQJPL[WXUHV
DVDIRRGVRXUFHIRUSROOLQDWRUV
Key words:EHHVJUD]LQJEXPEOHEHHPHGLFLQDOSODQWVSROOLQDWRUDWWUDFWLRQ

GENETIC DIVERSITY OF IMMORTELLE

(HELICHRYSUM ITALICUM /ROTH/ G. DON)
POPULATIONS IN CROATIA
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Grdiša3,4'HMDQ3OMHYOMDNXãLü5=ODWNRâDWRYLü3,4

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Belgrade, Serbia
Immortelle (Helichrysum italicum5RWK*'RQLVDSHUHQQLDOWKHUPRSKLORXVVXEVKUXEZLGHO\

GLVWULEXWHGLQWKH0HGLWHUUDQHDQEDVLQ,PPRUWHOOHHVVHQWLDORLOLVKLJKO\SUDLVHGKDYLQJDZLGH
UDQJHRIUHPDUNDEOHPHGLFLQDOSURSHUWLHV,QUHFHQW\HDUVPDVVLYHXQFRQWUROOHGFRPPHUFLDO
exploitation of wild immortelle populations prompts the Ministry of Environmental and
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DQGSRSXODWLRQVWUXFWXUHRILPPRUWHOOHSRSXODWLRQVIURP&URDWLDZHUHH[DPLQHGE\XVLQJ
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ZHUHLQ+DUG\:HLQEHUJHTXLOLEULXP:ULJKW¶VLQGH[RIJHQHWLFGLIIHUHQWLDWLRQZDVVLJQL¿FDQW
EXW ORZ VXJJHVWLQJ H[WHQVLYH JHQH ÀRZ DPRQJ SRSXODWLRQV 7KH DQDO\VLV RI PROHFXODU
variance indicated that the most of the genetic diversity was attributable to differences among
individuals within populations in concordance with the patterns expected for an outcrossing
DQGPRGHUDWHO\ORQJOLYHGVSHFLHV%D\HVLDQ$QDO\VLVRI3RSXODWLRQ6WUXFWXUH%$36UHVXOWHG
LQDVVLJQPHQWRIWKHLQYHVWLJDWHGVDPSOHVLQWZRFOXVWHUV6DPSOHVRULJLQDWLQJIURPSRSXODWLRQV
sampled in northern Adriatic were assigned to Cluster A, while the Cluster B included the
VDPSOHV EHORQJLQJ WR SRSXODWLRQV IURP WKH PLGGOH DQG VRXWKHUQ$GULDWLF7KH UHVXOWV ZHUH
FRPSDUHGZLWKWKRVHREWDLQHGIURP$)/3DQDO\VHVRI'DOPDWLDQVDJHSalvia RI¿FLQDOLV/
and Dalmatian pyrethrum (Tanacetum cinerariifolium 7UHYLU 6FK %LS SRSXODWLRQV IURP
Croatia in which the similar pattern of genetic diversity and population structure has been
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Key words: Helichrysum italicumPHGLFLQDODQGDURPDWLFSODQWVJHQHWLFGLYHUVLW\$)/3V

plant genetic resources

GENETIC DIVERSITY OF NATURAL POPULATIONS OF

SALVIA FRUTICOSA MILL. AND SALVIA POMIFERA L.
FROM PELOPONNESE BASED ON SSR MARKERS
Peggy Leontaritou1,2, Fotini Lamari3, Grigoris Iatrou2, Vasilis
Papasotiropoulos4

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5XUDO'HYHORSPHQWDQG)RRG3DQHSLVWLPLRX3DWUDV*UHHFH
2
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3
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Department of Agricultural Technology, Technological Educational Institute of Western
*UHHFH$PDOLDGD*UHHFH
S. fruticose 0LOONQRZQLQWKHLQWHUQDWLRQDOWUDGHDV*UHHNVDJHDQGS. pomifera /. NQRZQDV

&UHWDQVDJHERWKFDOOHGLQ*UHHFH³IDVNRPLOR´DUHWZRSalvia species growing naturally in
3HORSRQQHVH6RXWKHUQ*UHHFH7KH\DUHERWKXVHGDVUHPHGLHVDQGVSLFHVVLQFHDQFLHQWWLPHV
LQWKH0HGLWHUUDQHDQ7KHGHPDQGRIWKHDURPDWLFDQGPHGLFLQDOSODQWV¶LQGXVWU\IRUTXDOLW\
products, urges the need for development of plant propagating material for farmers to grow
V\VWHPDWLFDOO\DQGLQFRPSOLDQFHZLWKPRGHUQFXOWLYDWLRQSUDFWLFHV,QWKLVIUDPHWKHSXUSRVH
of this study, which is part of a bigger project, was to assess the genetic diversity existing among
ten natural populations of S. fruticosaDQG¿YHQDWXUDOSRSXODWLRQVRIS. pomifera growing in
3HORSRQQHVHXVLQJQLQH665PDUNHUVWKDWKDYHEHHQSUHYLRXVO\GHYHORSHGIRU6RI¿FLQDOLV
In S. fruticosa all nine and in S. pomifera eight out of nine SSR primers examined, were
VXFFHVVIXOO\DPSOL¿HG7KHWRWDOQXPEHURIUHYHDOHGDOOHOHVZDVDQGIRUS. fruticosa and
S. pomiferaUHVSHFWLYHO\:KLOHPRVWRIWKH665PDUNHUVVWXGLHGUHYHDOHGDOOHOHVSHUORFXV
there were three that were extremely polymorphic revealing a number of alleles, ranging from
WR,QS. fruticosaWKHREVHUYHGKHWHUR]\JRVLW\UDQJHGIURPWRDQGWKHH[SHFWHG
KHWHUR]\JRVLW\ IURP WR ZKLOH LQ S. pomifera IURP WR DQG IURP WR
UHVSHFWLYHO\1RWDEO\WKH3&R$ZDVDEOHWRVHSDUDWHWKHGLIIHUHQWVSHFLHVDQGPRUHRYHU
to group together S. fruticosa populations originating from neighboring geographical regions
RI3HORSRQQHVH0RUHRYHUDVVKRZQE\$029$KLJKPROHFXODUYDULDQFHZDVIRXQGZLWKLQ
SRSXODWLRQVDQGOHVVHUDPRQJWKHPLQERWKVSHFLHV
Key words: Salvia*UHHNVDJH&UHWDQVDJHPLFURVDWHOOLWHVSRSXODWLRQJHQHWLFV

CHEMOSYSTEMATICS OF THE CICHORIEAE TRIBE OF

THE ASTERACEAE FAMILY
Christian Zidorn
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Germany
The Cichorieae WULEH RI WKH $VWHUDFHDH IDPLO\ LV ZHOO FKDUDFWHUL]HG E\ D FRPELQDWLRQ RI
PLON\ ODWH[ DQG KRPRJDPRXV FDSLWXOD ZLWK ¿YH GHQWDWH OLJXODWH ÀRZHUV 7KH ODWH[ KDV D
ELWWHUWDVWHFDXVHGE\VHVTXLWHUSHQHODFWRQHVDQGSDUWLFXODUO\FKDUDFWHULVWLFIRUCichorieae,
WKHLU JOXFRVLGHV7KH WULEH HQFRPSDVVHV DERXW JHQHUD VSHFLHV DQG DGGLWLRQDOO\ D
multitude of micro - species in the genera Hieracium, Pilosella, and TaraxacumCichorieae
encompass medicinal plant species, such as Lactuca virosa/DQGHieracium pilosella /
3LORVHOODRI¿FLQDUXP/):6FKXOW] 6FK%LSDQGPDQ\ELWWHUWDVWLQJYHJHWDEOHVVXFK
as lettuce (Lactuca sativa / FKLFRU\ DQG UDGLFFKLR Cichorium intybus / DQG HQGLYH
(Cichorium endivia/$GGLWLRQDOVSHFLHVDUHFROOHFWHGDVYHJHWDEOHVLQWKHZLOGHJLactuca
alpine/:DOOU%HVLGHVVHVTXLWHUSHQHVWULWHUSHQHVFRXPDULQVÀDYRQRLGVSKHQROLFDFLGV
and more recently, stilbenoids have been found in the Cichorieae+RZHYHUFKHPLFDOGLYHUVLW\
LVJUHDWHVWLQWKHFODVVRIVHVTXLWHUSHQHODFWRQHV(DFKJHQXVVHHPVWREHFKDUDFWHUL]HGE\D
VSHFL¿FVHWRIFRPSRXQGVEHORQJLQJWRWKHVXEFODVVHVRIHXGHVPDQROLGHVJHUPDFUDQROLGHV
DQGRU JXDLDQROLGHV 7KH &LFKRULHDH HYHQ IHDWXUH RQH JURXS RI VHVTXLWHUSHQH ODFWRQHV
K\SRFUHWHQROLGHVLHJXDLDQROLGHVZKLFKKDVVRIDURQO\KDYHEHHQIRXQGLQPHPEHUVRI
the Cichorieae, and here only in the HypochaeridinaeVXEWULEH&KHPRV\VWHPDWLFVWXGLHVFDQ
QRWRQO\EHXWLOL]HGWRFKDUDFWHUL]HWD[DDWGLIIHUHQWOHYHOVVXFKDVVSHFLHVRUJHQHUDWKH\FDQ
DOVRDLGLQWKHLGHQWL¿FDWLRQRISDUHQWDOVSHFLHVRIK\EULGVLeontodon x grassiorum Zidorn, a
new Leontodon VVWUK\EULGZDVUHFHQWO\GLVFRYHUHGLQ$XVWULD7KHQRWKRVSHFLHVSURYHGWREH
DK\EULGEHWZHHQDQ$]RUHDQHQGHPLFLeontodon species, Leontodon hochstetteri00RXUD
/ 6LOYD DQG L. hispidus / 7KH QHZ K\EULG ZDV PRUSKRORJLFDOO\ DQG SK\WRFKHPLFDOO\
FKDUDFWHULVHG 0RUHRYHU PROHFXODU SK\ORJHQHWLF VWXGLHV ZHUH SHUIRUPHG 7R XQUDYHO WKH
H[DFWRULJLQRIWKHK\EULGDFRPELQDWLRQRIDOOWKUHHGDWDVHWVZDVQHFHVVDU\7KHREVHUYHG
SDWWHUQVZLOOEHGLVFXVVHGZLWKVSHFLDOHPSKDVLVRQWKHLPSRUWDQFHRIFKHPLFDOPDUNHUVLQWKH
GLVFRYHU\DQGFKDUDFWHULVDWLRQRIK\EULGV
Key words:$VWHUDFHDHFKHPRV\VWHPDWLFVVHVTXLWHUSHQHODFWRQHV

AN ANALYSIS ON WILD EDIBLE PLANTS OF THE

TURKISH ASTERACEAE TAXA
øVPDLOùHQNDUGHú*L]HP%XOXW$KPHW'R÷DQ(UWDQ7X]ODFÕ
University of Marmara, Faculty of Pharmacy, Department of Pharmaceutical Botany,

7ÕEEL\H6WUHHW1RøVWDQEXO7XUNH\
$VWHUDFHDH LV RQH RI WKH ODUJHVW IDPLOLHV LQ WKH )ORUD RI7XUNH\$OVR LW LV RQH RI WKH PRVW
important plant families in which there are many popular plants mainly used as food and
WHDLQ7XUNH\EHFDXVHRIWKHHVVHQWLDORLOVFRQWHQW7KHDLPRIWKLVVWXG\LVWKHDQDO\VLVRI
WKHZLOGHGLEOHSODQWVRIWKH$VWHUDFHDHIDPLO\LQ7XUNH\EDVHGRQRXUORFDOHWKQRERWDQLFDO
UHVHDUFK DQG DYDLODEOH VFLHQWL¿F OLWHUDWXUH 7KH LQIRUPDWLRQ DERXW WKH SODQWV ZDV REWDLQHG
IURP WKH ORFDO SHRSOH WKURXJK RSHQ DQG VHPLVWUXFWXUHG LQWHUYLHZV $FFRUGLQJ WR WKH OLVW
EDVHGRQRXULQYHVWLJDWLRQVDQGWKHOLWHUDWXUHUHFRUGVJHQHUDLQFOXGLQJWD[DRIWKH
IDPLO\$VWHUDFHDHLVXVHGIRUIRRGWHDDQGRWKHUSXUSRVHV$PRQJWKHPAchillea, Centaurea,
Gundelia, Onopordum, Scorzonera and Tragopogon are the most popular genera in the
ORFDOLWLHVRI7XUNH\
Key words:$VWHUDFHDHZLOGHGLEOHSODQWV7XUNH\

GENETIC BACKGROUND OF DALMATIAN

PYRETHRUM (TANACETUM CINERARIIFOLIUM /Trevir./
Sch. Bip.) INSECTICIDAL POTENTIAL
Martina Grdiša1,5, Filip Varga1,50DULMD-XJ'XMDNRYLü2, Tonka
1LQþHYLü2, Zlatko Liber3,5,YDQ5DGRVDYOMHYLü3,50DUWLQD%LRãLü4, Zlatko
âDWRYLü1,5

Faculty of Agriculture, University of Zagreb, 6YHWRãLPXQVNDFHVWD=DJUHE&URDWLD
2
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0DUXOLüHYWUJD=DJUHE&URDWLD

University of Zagreb, Faculty of Chemical Engineering and Technology, 0DUXOLüHYWUJ
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5
Centre of Excellence for Biodiversity and Molecular Plant Breeding, 6YHWRãLPXQVNDFHVWD
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3\UHWKULQLVWKHVHFRQGDU\PHWDEROLWHV\QWKHVL]HGLQ'DOPDWLDQS\UHWKUXPTanacetum cinerariifolium
7UHYLU 6FK %LS DQG UHFRJQL]HG IRU LWV SRWHQW LQVHFWLFLGDO DFWLYLW\ ZLWK QR DGYHUVH HIIHFWV RQ
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WKUHDWHQHGE\DQWKURSRJHQLFKDELWDWORVVDQGGHJUDGDWLRQUHODWHGWRIDFWRUVVXFKDVXUEDQL]DWLRQDQG
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*D]HWWH'HVSLWHLWVKLVWRULFDOVLJQL¿FDQFHDQGHFRQRPLFYDOXHOLPLWHGJHQHWLFDQG
ELRFKHPLFDO GDWD RQ 'DOPDWLDQ S\UHWKUXP LV DYDLODEOH 7KHUHIRUH WKH LPSRUWDQFH RI WKLV SURMHFW
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are: (i GHYHORSPHQW RI PLFURVDWHOOLWH SULPHUV 665 6LPSOH 6HTXHQFH 5HSHDWV IRU 'DOPDWLDQ
S\UHWKUXPiiXWLOL]DWLRQRIQHZO\GHYHORSHG665DQG$PSOL¿HG)UDJPHQW/HQJWK3RO\PRUSKLVP
$)/3PDUNHUVLQDVVHVVLQJJHQHWLFGLYHUVLW\RI'S\UHWKUXPQDWXUDOSRSXODWLRQViiiRSWLPL]DWLRQ
RI0DWULF6ROLG3KDVH'LVSHUVLRQ063'WHFKQLTXHDQGLWVXWLOL]DWLRQLQWKHH[WUDFWLRQRIS\UHWKULQV
DQGLGHQWL¿FDWLRQDQGGHWHUPLQDWLRQRIVL[H[WUDFWHGS\UHWKULQFRPSRQHQWVLQVDPSOHGSRSXODWLRQV
ZLWK+LJK3HUIRUPDQFH/LTXLG&KURPDWRJUDSK\'$'+3/&'$'ivLGHQWL¿FDWLRQRI$)/3
PDUNHUVOLQNHGWRWKHWUDLWORFLUHODWHGWRWKHFRQWHQWRIHDFKS\UHWKULQFRPSRQHQWWKURXJK$VVRFLDWLRQ
Mapping studies which will facilitate the management of natural populations in future breeding
SURJUDPPHV EDVHG RQ 0DUNHU$VVLVWHG 6HOHFWLRQ 0$6 (PHUJLQJ WHFKQRORJLHV DQG PHWKRGV
proposed in this project will lead to the development of new approaches to biodiversity monitoring,
plant genetic resources conservation, future breeding programmes and agricultural exploitation of
'DOPDWLDQS\UHWKUXP
Keywords: association mapping, biochemical diversity, Dalmatian pyrethrum, genetic diversity,
insecticide, MSPD, pyrethrin, Tanacetum cinerariifolium
Acknowledgment: This work has been fully supported by the Croatian Science Foundation under
the project ‘Genetic background of Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir. / Sch.
Bip.) insecticidal potential’ - (PyrDiv) (IP-06-2016-9034).

Book of abstracts – 10th CMAPSEEC Poster presentations
PREDICTING SUITABLE HABITAT FOR DALMATIAN

PYRETHRUM (TANACETUM CINERARIIFOLIUM /Trevir.
/ Sch. Bip.) IN CROATIA
Filip Varga1,3,YDQ5DGRVDYOMHYLü2,3, Martina Grdiša1,3=ODWNRâDWRYLü1,3

2
0DUXOLüHYWUJD=DJUHE&URDWLD
3
+5=DJUHE&URDWLD
Dalmatian pyrethrum (Tanacetum cinerariifolium7UHYLU6FK%LSLVDWKHUPRSKLOLFSODQW

species of the Asteraceae family naturally distributed on the East Adriatic coast and its offshore
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QHHGWRGHWHUPLQHSRWHQWLDOQHZORFDOLWLHVZKHUHWKLVVSHFLHVFDQEHIRXQG7KHDLPRIWKLV
study was to combine all distribution data available for Dalmatian pyrethrum in Croatia and to
SUHGLFWSRWHQWLDOORFDOLWLHVIRUIXWXUHHFRJHRJUDSKLFDOVXUYH\V7KHGDWDZDVPDLQO\REWDLQHG
IURP¿HOGVWXGLHVFRQGXFWHGLQWKHSHULRGIURPWRDVZHOODVIURPDYDLODEOHVFLHQWL¿F
OLWHUDWXUHDQGKHUEDULXPFROOHFWLRQV$WRWDORIORFDOLWLHVRIKLJKJHRJUDSKLFFRRUGLQDWHV
SUHFLVLRQZHUHUHFRUGHGPRVWIUHTXHQWO\LQJDUULJXHDQGGU\JUDVVODQGV0D[(QWVRIWZDUHZDV
XVHGWRSUHGLFWHFRORJLFDOO\VXLWDEOHUDQJHVEDVHGRQELRFOLPDWLFYDULDEOHVREWDLQHGIURP
WKH:RUOG&OLPGDWDVHW7KHPRVWVXLWDEOHJHRJUDSKLFDODUHDVIRUT. cinerariifolium in Croatia
are located along the coastline and on the islands with exception of the most northern part of
,VWULD7KHPRGHODOVRUHYHDOHGVRPHVXLWDEOHDUHDVLQ%RVQLDDQG+HU]HJRYLQD0RQWHQHJUR
DQG$OEDQLD,VRWKHUPDOLW\DQG0HDQ7HPSHUDWXUHRI'ULHVW4XDUWHUKDGWKHKLJKHVWLQÀXHQFH
RQWKHSRWHQWLDOGLVWULEXWLRQRIWKLVVSHFLHV7KHLQIRUPDWLRQREWDLQHGZLOOEHXVHGLQSODQQLQJ
IXWXUH¿HOGVWXGLHVDVZHOODVEXLOGLQJSUHGLFWLRQPRGHOVIRUGLVWULEXWLRQRIWKHVSHFLHVLQWKH
IXWXUHGXHWRFOLPDWHFKDQJH
Key words: suitable habitat modelling, Tanacetum cinerariifoliumPD[HQWPRGHO%DONDQ

Peninsula
Acknowledgment: This work has been fully supported by the Croatian Science Foundation
under the project ‘Genetic background of Dalmatian pyrethrum (Tanacetum cinerariifolium /
Trevir./ Sch. Bip.) insecticidal potential’ - (PyrDiv) (IP-06-2016-9034).

ASSESSING THE MEDICINAL AND AROMATIC PLANTS

IN WATERSHED OF THE MIDDLE SECTION OF
DEVOLLI RIVER
Marjol Meço1, Alfred Mullaj2

University of Tirana, Faculty of Natural Science, Department of Biology, Tirana, Albania
2
University of Tirana, Faculty of Natural Science, Research Center for Flora and Fauna,
Tirana, Albania
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medicinal and aromatic plants (MAPs) in watershed of the middle section of the Devolli River,
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EHORQJVWR0$3V7KHVHSODQWVEHORQJWRJHQHUDDQGIDPLOLHVPDLQO\WRWKH/DPLDFHDH
$VWHUDFHDH5RVDFHDHDQG)DEDFHDH+HPLFU\SWRSK\WHVWD[DIROORZHG
E\3KDQHURSK\WHWD[DDUHWKHPRVWDEXQGDQWOLIHIRUPV$KLJKHUSHUFHQWDJHRIWKHWD[D
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FRQVHUYDWLRQFRQFHUQWKDWDUHSURWHFWHGE\1DWLRQDO/HJLVODWLRQRULQWKH1DWLRQDO5HG'DWD
%RRN$FFRUGLQJWRWKHORFDOLQIRUPDWLRQDQGWKHGDWDREWDLQHGWKURXJKWKHLQWHUYLHZVZLWK
WKHFRPSDQLHVZKLFKJDWKHUSODQWVLQWKLVDUHDODUJHUTXDQWLWLHVRIWKHVHVSHFLHVDUHH[SRUWHG
PRVWO\LQ86$DQG*HUPDQ\DQGIURPWKHPLWLVJHQHUDWHGDERXWSHU\HDU0RUH
WKDQ RI WKLV YDOXH FRPHV IURP H[SRUWDWLRQ RI Lavandula angustifolia 0LOO DQG IURP
other wild MAPs such are Primula veris /., Juniperus communis /., J. oxycedrus /., Satureja
montana/Origanum vulgare /., Cistus incanus/DQGSideritis raeseri%RLVVDQG+HOGU7KH
FXOWLYDWLRQRI0$3VLVEHFRPLQJLQFUHDVLQJO\LPSRUWDQWLQWKLVUHJLRQ&XUUHQWO\Lavandula
angustifolia0LOOGRPLQDWHVWKHFXOWLYDWHG0$3V6PDOOHUVXUIDFHVDUHDOVRFXOWLYDWHGZLWK
6DOYLDRI¿FLQDOLV/., Cyanus segetum/5RVPDULQXVRI¿FLQDOLV/HWF7KLVVWXG\VKRZVWKDW
VWRFNVRI0$3VLQWKHZDWHUVKHGRIWKHPLGGOHVHFWLRQRIWKH'HYROOL5LYHUKDYHGHFOLQHGLQ
the past decades with some species becoming rare or endangered due to habitat loss, habitat
PRGL¿FDWLRQDQGRYHUH[SORLWDWLRQ
Key words: watershed, Devolli River, MAPs

THE MEDICINAL AND WILD FOOD PLANTS OF

BATMAN CITY CENTER AND KOZLUK DISTRICT
(BATMAN-TURKEY)
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7X]ODFÕ
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hVNGDU,VWDQEXO7XUNH\
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XVHGDVWUDGLWLRQDOIRONPHGLFLQHLQWKHUHJLRQDQGHOHYHQRIWKHVHDUHDOVRXVHGDVDVRXUFHRI
ZLOGIRRG)XUWKHUPRUHQLQHWD[DDUHZLOGVRXUFHVIRURWKHUHWKQRERWDQLFDOXVDJHVIRUWKHDUHD
The plants most commonly used in the region as medicinal remedies are Malva nicaeensis
$OOPistacia khinjuk 6WRFNVPlantago majorVXEVSintermedia*LOLEDQGTeucrium polium/
Plants are mostly used for the treatment of gastrointestinal system diseases, respiratory system
GLVHDVHVDQGGLDEHWHV7KHVSHFLHVPRVWFRPPRQO\XVHGIRUIRRGDUHGundelia tournefortii
/Pistacia khinjuk 6WRFNVDQGRhus coriaria/7KLVHWKQRERWDQLFDOVWXG\FRQGXFWHGLQERWK
districts will enable the traditional use of wild plants both as food sources and herbal remedies
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Key words:HWKQRERWDQ\PHGLFLQDOSODQWV.R]OXN%DWPDQ7XUNH\

MEDICINAL PLANTS TRADITIONALLY USED IN THE

TREATMENT OF DIABETES IN THE REGION OF SUVA
PLANINA MOUNTAIN (SOUTHEASTERN SERBIA)
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Serbia
2
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UHVRXUFHIRUDODUJHPDMRULW\RIWKHSHRSOHRIWKLVUXUDOUHJLRQIRUWKHLUSULPDU\KHDOWKFDUH
7KLV LV GXH WR WKH VSHFL¿F JHRJUDSKLFDO SRVLWLRQ WKH KLJK ELRGLYHUVLW\ HWKQLF DQG FXOWXUDO
FKDUDFWHULVWLFV DQG IRON WUDGLWLRQ7KLV HWKQRERWDQLFDO VXUYH\ ZDV XQGHUWDNHQ WR LQYHVWLJDWH
and document the anti-diabetic plant species used by the local people living on Suva Planina
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SRWHQWLDOGHYHORSPHQWRIQHZHIIHFWLYHDQGVDIHPHGLFLQHV7KHVWXG\ZDVEDVHGRQWKH¿HOG
survey and details about the herb collection, mode of preparation of herbal remedies, treatment
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were collected from the locals through semi structured interviews, group discussions and
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$ WRWDO RI SODQW VSHFLHV DQG WZR IXQJL DUH WUDGLWLRQDOO\ XVHG IRU PHGLFLQDO SXUSRVHV
LQ WKH LQYHVWLJDWHG UHJLRQ RI ZKLFK DUH XVHG LQ WKH WUHDWPHQW RI GLDEHWHV The
PRVW IUHTXHQWO\ PHQWLRQHG DQWLGLDEHWLF PHGLFLQDO SODQWV ZHUH Alchemilla vulgaris / WKH
whole plant), Allium ampeloprasum / DHULDO SDUW Centaurium umbellatum *LOLE DHULDO
part), Gentiana cruciata/KHUEHelianthus tuberosus/IUHVKUKL]RPHJuglans regia/
(internal hardened part of endocarp), Mentha piperita/DHULDOSDUWMorus alba/OHDI
Petroselinum crispum0LOO$:+LOOOHDIDQGURRWDQGSorbus domestica/IUXLW)RU
PHGLFLQDOSXUSRVHVWKHIUHVKRUGULHGDHULDORUXQGHUJURXQGSODQWSDUWVLQFOXGLQJWKHUKL]RPH
DUHXVHGDQGJHQHUDOPHWKRGIRUSUHSDULQJKHUEDOUHPHGLHVLQFOXGHVLQIXVLRQDQGGHFRFWLRQ
5LFK SODQW GLYHUVLW\ FRPELQHG ZLWK ORFDO VNLOOV H[SHULHQFHV DQG NQRZOHGJH RI WKH KHDOLQJ
SURSHUWLHVRIKHUEVLQWKHUHJLRQRI6XYD3ODQLQD0WUHSUHVHQWDQLQYDOXDEOHKHULWDJHWREH
SUHVHUYHGIRUIXWXUHJHQHUDWLRQV
Key words: medicinal plants, diabetes, Suva Planina Mountain (Southeastern Serbia)

ETHNOMEDICINAL STUDY OF PREPARATIONS

OBTAINED FROM JUGLANDIS IMMATURI FRUCTUS
FROM THE FOLK AND TRADITIONAL MEDICINE OF
REPUBLIC OF MACEDONIA
Biljana Bauer1, Biljana Chuleva2, Ana Kocevska3

)DFXOW\RI3KDUPDF\8QLYHUVLW\RI6V&\ULODQG0HWKRGLXV0DMND7HUH]D1R
6NRSMH5HSXEOLFRI0DFHGRQLD
2
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3
6SHFLDO+RVSLWDOIRU*\QHFRORJ\DQG2EVWHWULFV?´0RWKHU7HUHVD?´&KDLU6NRSMH
Republic of Macedonia
Republic of Macedonia has old ethnomedicine traditions, consisting of many recipes with
KHUEDODQLPDODQGPLQHUDORULJLQDOLQJUHGLHQWV7KHIRONDQGWUDGLWLRQDOPHGLFLQHRI5HSXEOLF
RI0DFHGRQLDSD\VVSHFLDODWWHQWLRQWRGLVHDVHSUHYHQWLRQ,WLVLQWHUHVWLQJWRPHQWLRQDQLFH
practice in Republic of Macedonia: conserving of the juglandis immaturi fructus in honey as
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ZLWKZHDNVWRPDFK2QHOLWWOHVSRRQKRQH\DQGRQHZDOQXWDUHXVHGLQVSULQJSHULRGHYHU\
PRUQLQJ DJDLQVW ZHDNQHVV WXEHUFXORVLV DQG VFURIXOD DW JODQGXUDO WXEHUFXORVLV ,W LV XVHG
JHQHUDOO\ IRU LPPXQLW\$OVR juglandis immaturi fructus DUH VWRUHG LQ UDNL IRU ZHHNV RQ
VXQRULQDZDUPSODFH$IWHUWKDWWKHOLTXLGLV¿OWUDWHGDQGXVHGDFFRUGLQJWRWKHQHHGRIWKH
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IRU UHPRYLQJ WKH ZHDNQHVV RI WKH RUJDQLVP UHPRYLQJ EDFWHULD IURP WKH LQWHVWLQH DQG IRU
EDODQFHVWKHYLVFRXVGHQVLW\RIWKHEORRG:DOQXWVLQWKHVHUHFLSHVDUHQRWKHDWDQGWKHUHLV
QRKDUPIXOHIIHFWIURPKHDW7KHVHXVHIXOFXVWRPVDUHFRQWLQXLQJWREHH[SDQGHGDWHYHU\VWHS
E\WKHSHRSOHDQGYDOXHGDVJRRGROGPHGLFDOUHFHLSWV,WVKRXOGEHFKHPLFDOO\DQGFOLQLFDOO\
H[DPLQHGKRZYLWDPLQVDUHSUHVHUYHGLQWKDWFDQ,QWKLVZD\³VZHHWÝLWDPLQ&PJ
JYLWDPLQ'PJJVRGLXPPJJFDOFLXPPJJDQGUDNL
YLWDPLQ& PJJ YLWDPLQ ' PJJ VRGLXPPJJ, potassium
PJJFDOFLXPPJJRIWKHZDOQXWVLVRXUEHVWIRONFDQRIYLWDPLQVDQG
RWKHUPHGLFLQDOLQJUHGLHQWV
Key words:KRQH\YLWDPLQVUDNLPLQHUDOV

DEMOGRAPHIC CHARACTERISTICS OF THE LOCAL

POPULATION IN CERTAIN SETTLEMENTS ON THE
NORTH BLACK SEA COAST (BULGARIA) IN THE USE
OF MEDICINAL PLANTS
Viktoria Marinova
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7KH SXUSRVH RI WKLV VWXG\ ZDV WR HVWDEOLVK WKH GHPRJUDSKLF LQGLFDWRUV DQG WR WDNH LQWR
account the peculiarities of the medicinal plants use in human and veterinary medicine by
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ZHUH VWXGLHG $NVDNRYR DQG 7RSROL 9DUQD GLVWULFW 7KH VXUYH\ ZDV FRQGXFWHG EHWZHHQ
-XQH DQG$XJXVW ,QWHUYLHZV ZLWK WKH ORFDO SRSXODWLRQ ZHUH FRQGXFWHG XVLQJ RULJLQDO
TXHVWLRQQDLUHVSUHSDUHGXSIURQW7KHUHVSRQGHQWVEHORQJHGWRGLIIHUHQWJHQGHUHWKQLFLW\DJH
DQGHGXFDWLRQJURXSV7KHGDWDRQKRZWRJDWKHUPHGLFLQDOSODQWVUHFRPPHQGDWLRQVIRUXVH
GUDZEDFNVIUHTXHQF\RIXVHRIPHGLFLQDOSODQWVDUHFRPSDUHG
Key words:PHGLFLQDOSODQWVHWKQRERWDQ\1RUWKHUQ%ODFN6HDFRDVW

INVESTIGATION OF MEDICINAL PLANTS ON

THE TERRITORY OF PETROHAN TRAINING AND
EXPERIMENTAL FOREST RANGE (WESTERN STARA
PLANINA, BULGARIA)
Violeta Dimitrova, Alexander Tashev
8QLYHUVLW\RI)RUHVWU\)DFXOW\RI)RUHVWU\'HSDUWPHQWRI'HQGURORJ\.OLPHQW2KULGVNL
%OYG6R¿D%XOJDULD
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VLJQL¿FDQWVFLHQWL¿FDQGSUDFWLFDOLPSRUWDQFHUHODWHGWRWKHFRQVHUYDWLRQRIWKHLUUHVRXUFHV
DQG WKHLU VXVWDLQDEOH XVH 7KH SUHVHQW VWXG\ SUHVHQWV UHVXOWV RI WKH LQYHQWRU\ RI PHGLFLQDO
plants on the territory of the Petrohan Training and Experimental Forest Range (Western Stara
3ODQLQD%XOJDULD$WRWDORIVSHFLHVRIYDVFXODUSODQWVEHORQJLQJWRJHQHUDDQG
IDPLOLHV ZHUH GHVFULEHG &KDUDFWHULVWLF RI WKH V\VWHPDWLF VWUXFWXUH RI WKH VWXGLHG SODQW ZDV
GRQHDQGWKHLUFODVVL¿FDWLRQDFFRUGLQJWRELRORJLFDOW\SHOLIHIRUPÀRULVWLFJHROHPHQWVSHULRG
RIÀRZHULQJDQGHFRORJLFDOJURXSLVSUHVHQWHG7KHHFRORJLFDODQGELRORJLFDOFKDUDFWHULVWLF
as well as information on the usable parts of the medicinal plants and the biologically active
VXEVWDQFHVFRQWDLQHGDUHSUHVHQWHG7KHLQIRUPDWLRQJDWKHUHGGXULQJWKHVWXG\LVDSUHUHTXLVLWH
IRU IXWXUH UHVRXUFH UHVHDUFK 6SHFLI\LQJ VSHFLHV FRPSRVLWLRQ DQG UHVRXUFH VWDWXV ZLOO
FRQWULEXWHWRWKHLUDVVHVVPHQWDVDQHFRV\VWHPVHUYLFHRIIHUHGE\IRUHVWFRPPXQLWLHV)LQDOO\
we present a systematic list of the medicinal plants on the territory of the Petrohan Training
DQG([SHULPHQWDO)RUHVW5DQJH
Key words: medicinal plants, systematic, ecobiological analysis

PRELIMINARY STUDY OF THE FOLK MEDICINAL

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øVPDLOùHQNDUGHú$KPHW'R÷DQ*L]HP%XOXW
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YLVLWHG 7KH VSHFLPHQV RI WKH SODQWV XVHG DV IRON UHPHGLHV KDYH EHHQ FROOHFWHG DQG WKH
information about the local names, the part(s) used, the ailments treated, the therapeutic effect,
the preparation, the methods of administration and the duration of treatment were obtained
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ZKLFKDUHWKHPDWHULDOVRIWKLVVWXG\ZHUHLGHQWL¿HGXVLQJWKH³)ORUDRI7XUNH\DQGWKH(DVW
$HJHDQ ,VODQGV´ DQG WKH\ DUH NHSW LQ WKH +HUEDULXP RI WKH )DFXOW\ RI 3KDUPDF\ 0DUPDUD
8QLYHUVLW\0$5($FFRUGLQJWRWKHUHVXOWVRIRXUHWKQRERWDQLFDOVWXG\WD[DLVXVHGDV
DWUDGLWLRQDOIRONPHGLFLQHLQ7DúN|SU$FFRUGLQJWRWKHPDMRULW\RIWKHSODQWVZKLFKKDYH
similar usage, the plants are mostly used for respiratory, integumentary system, circulatory
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Key words:7DúN|SUWUDGLWLRQDOIRONPHGLFLQHHWKQRERWDQ\

GENETIC DIVERSITY OF HELLEBORUS ODORUS

Waldst. & Kit. subsp. CYCLOPHYLLUS (A. Braun) Maire &
Petitm. POPULATIONS IN GREECE
Georgia Fassou1, Gregoris Iatrou1, Fotini Lamari2, Vassilis
Papasotiropoulos3

/DERUDWRU\RI%RWDQ\'HSDUWPHQWRI%LRORJ\8QLYHUVLW\RI3DWUDV3DWUDV*UHHFH
/DERUDWRU\RI3KDUPDFRJQRV\ &KHPLVWU\RI1DWXUDO3URGXFWV'HSDUWPHQWRI3KDUPDF\
2
University of Patras, Patras, Greece

3
Department of Agricultural Technology, Technological Educational Institute of Western
*UHHFH$PDOLDGD*UHHFH
Helleborus odorus:DOGVW .LWVXEVScyclophyllus $%UDXQ0DLUH 3HWLWPLVNQRZQ

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RIPHPRU\DQGDOVRXVHGDVSDLQNLOOHUDQGDQWLGHSUHVVDQWVLQFHWKHWKFHQWXU\5HFHQWVWXGLHV
demonstrate that its main compounds hellebrin and hellebrigenin may have an anticancer effect
DQGFDQEHXVHGDVGUXJV In the current study, genetic diversity of eight different populations
of H. odorus VXEVS cyclophyllus, covering a great range of this subspecies geographical
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DOVRFRQ¿UPHGE\WKH83*0$GHQGURJUDP0RUHRYHUWKHKLJKHVWOHYHORIJHQHWLFGLYHUVLW\
ZDVIRXQGLQ9HORXKLSRSXODWLRQǿ 33% + ZKLOHWKHORZHVWLQWKH
SRSXODWLRQIURP0W2O\PSXVǿ 33% +
Key words: Helleborus, ISSR, genetic diversity

SOME INSIGHTS INTO PROPERTIES OF BRYOPHYTES

AS NEGLECTED MEDICINAL PLANTS
0DUNR66DERYOMHYLü0LORUDG9XMLþLü0DULMDûRVLü$QHWD6DERYOMHYLü
Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade,

7DNRYVND%HOJUDGH6HUELD
Bryophytes DUH WKHVHFRQG ELJJHVWJURXS RI ODQG SODQWVDIWHUDQJLRVSHUPV+RZHYHUWKH\

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GXH WR WKHLU VL]H DQG VPDOO ELRPDVV SURGXFWLRQ +RZHYHU WKHUH DUH H[DPSOHV LQ WUDGLWLRQDO
PHGLFLQHWRVRPHH[WHQWLQGLIIHUHQWZRUOGDUHDVBryophytes are very diverse group and many
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KDYH QHYHU EHHQ VWXGLHG7KH WHVW DQG DQDO\VHV RI PHGLFLQDO XVH DQG FKHPLFDO FRQVWLWXHQWV
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In vitro HVWDEOLVKPHQW JURZWK DQG \LHOG RSWLPL]DWLRQ DOORZ XV WR VWXG\ WKHVH SURSHUWLHV LQ
PDQ\QRWRQO\ELRPDVVOLPLWHGEXWDOVRUDUHDQGWKUHDWHQHGVSHFLHV&DVHVWXGLHVLQYDULRXV
VSHFLHV ZLOO EH SUHVHQWHG HJ RUJDQLF XQVDWXUDWHG DFLGV VXJDUV SKHQROV DQG VRPH RWKHU
FRPSRXQGVLQGLIIHUHQWVSHFLHV6RPHRIWKHPZLOOEHHPSKDVL]HGDVSRWHQWLDOVRXUFHRIUDUH
DQGLQWHUHVWHGFKHPLFDOFRQWHQWHJSRO\XQVDWXUDWHGRPHJDIDWW\DFLGLQMnium hornum
+HGZ WULVDFFKDULGH NHVWRVH LQ Rhodobryum ontariense .LQGE .LQGE RU DQWLWXPRU
ELVELEHQ]LOHVPDUFKDQWLQVLQWKDOORVHOLYHUZRUWMarchantia polymorpha /+DUYHVWWLPH
RUHQYLURQPHQWDOIDFWRUVFDQVLJQL¿FDQWO\DIIHFWWKHWDUJHWFRPSRXQG\LHOGDVZHOODVHIIHFWRI
FUXGHH[WUDFWLQWHVWHGSKHQRPHQD$QRWKHUVLJQL¿FDQWYDOXHRIbryophytes is the potential use
LQ PDQ\ ELRWHFKQORJLFDO SURFHVVHV ZKHUH ELRSHVWLFLGHV DQGRU VXEVWDQFH ZLWK DQWLIHHGLQJ
HIIHFWVFDQEHH[WUDFWHG
Key words: bryophytes, chemistry, medicinal plants

ETHNOBOTANY OF MEDICINAL PLANTS USED IN

SOME PARTS OF THE NORTHERN BLACK SEA COAST
REGION (BULGARIA)
Petya Boycheva
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This study is part of an ethnobotanical investigation of the medicinal plants in the Northern
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RI2E]RU7KHERXQGDULHVZHUHGHWHUPLQHGXVLQJWKHPDSRIWKHÀRULVWLFUHJLRQVLQ%XOJDULD
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2 communities ZHUHVXUYH\HG7KHVWXG\ZDVFDUULHGRXWEDVHGRQWKHVXUYH\PHWKRGRORJ\
,QWHUYLHZV ZLWK WKH ORFDO SHRSOH ZHUH FRQGXFWHG XVLQJ RULJLQDO TXHVWLRQQDLUHV SUHSDUHG
XSIURQW7KHUHVSRQGHQWVEHORQJHGWRGLIIHUHQWJHQGHUHWKQLFLW\DJHDQGHGXFDWLRQJURXSV
7KLVZRUNSUHVHQWVWKHUHVXOWVRIVXUYH\VLQVPDOOFRPPXQLWLHVRQO\VLQFHWKH¿HOGZRUNLV
QRWRYHU\HW7KHGDWDIURPWKHGLIIHUHQWDSSOLFDWLRQVRIPHGLFLQDOSODQWVLQWKHOLIHRIWKHORFDO
SHRSOHZHUHSURFHVVHGDQGVXPPDUL]HG
Key words:HWKQRERWDQ\HWKQRPHGLFLQHHWKQRYHWHULQDU\1RUWK%ODFN6HDFRDVW

REPRODUCTIVE BIOLOGY OF THE ENDANGERED

BULGARIAN ENDEMIC CENTAUREA ACHTAROVII
Urum. (ASTERACEAE)
Elina Yankova-Tsvetkova, Ivalena Ilieva, Stoyan Stoyanov, Boriana
Sidjimova, Marina Stanilova
,QVWLWXWHRI%LRGLYHUVLW\DQG(FRV\VWHP5HVHDUFK%XOJDULDQ$FDGHP\RI6FLHQFHV$FDG
*%RQFKHYVWU6R¿D%XOJDULD
Centaurea is the fourth largest genus of the family Asteraceae comprising hundreds of species,
PDQ\ RI WKHP XVHG DV PHGLFLQDO SODQWV Centaurea achtarovii 8UXP LV D ORFDO %XOJDULDQ
endemic, distributed in Pirin Mountain, protected by the Biodiversity Act and included in the
5HG 'DWD %RRN RI %XOJDULD DV ³HQGDQJHUHG´7KH SUHVHQW VWXG\ UHSRUWV WKH GDWD RI FXUUHQW
PRQLWRULQJDQGWKH¿UVWUHVXOWVRQWKHUHSURGXFWLYHELRORJ\RIC. achtarovii concerning both
SRSXODWLRQVRIWKLVSRRUO\VWXGLHGVSHFLHVDURXQG2UHOHNVXPPLWDQGDW.D]DQLWHORFDOLW\7KH
PRQLWRULQJZDVFRQGXFWHGLQVDPSOLQJSORWVRIVTXDUHPHWHUVLQHDFKSRSXODWLRQ%RWK
SRSXODWLRQVZHUHLQDYHU\JRRGFRQGLWLRQ3RSXODWLRQRI2UHOHNZDVODUJHUDQGZLWKKLJKHU
GHQVLW\WKDQWKDWRI.D]DQLWHKDLQGLYLGXDOVSHUP2YHJHWDWLYHDQGJHQHUDWLYH
DQGKDLQGLYLGXDOVSHUP2YHJHWDWLYHDQGJHQHUDWLYHUHVSHFWLYHO\)ORZHUEXGV
DQGRSHQÀRZHUVLQGLIIHUHQWVWDJHVRIGHYHORSPHQWZHUHWUHDWHGDFFRUGLQJWRFODVVLFDOSDUDI¿Q
PHWKRGV LQ RUGHU WR UHYHDO WKH SDUWLFXODULWLHV RI WKH UHSURGXFWLYH VSKHUH ,W ZDV HVWDEOLVKHG
that the structures in male and female generative spheres were stable, the processes leading to
IRUPDWLRQRIHPEU\RVDQGVHHGVZHUHEDODQFHGDQGDSRPL[HVGLGQRWRFFXU$OOWKHVHIHDWXUHV
GHWHUPLQHGWKHORZSODVWLFLW\RIWKHVSHFLHV3ROOHQYLDELOLW\DVVHVVHGE\DFHWRFDUPLQHWHVWZDV
UHODWLYHO\KLJK±RYHUZKLOHVHHGYLDELOLW\HVWLPDWHGE\WHWUD]ROLXPWHVWZDVORZ
In vitroVHHGJHUPLQDWLRQZDVH[WUHPHO\SRRU7KHORZSODVWLFLW\RIPDOHDQGIHPDOHJHQHUDWLYH
spheres decreased the adaptive mechanisms of C. achtaroviiWRWKHHQYLURQPHQWDOFRQGLWLRQV
The low viability of seeds, and the small amount of pollen, although with relatively high
IHUWLOLW\GHWHUPLQHGDORZUHSURGXFWLYHFDSDFLW\RIWKHVSHFLHV7KHUHIRUHin situ measures
OLNHUHGXFLQJJuniperus sibirica%XUJVGFRYHUDJHLVPRUHDSSURSULDWHIRUFRQVHUYDWLRQRIC.
achtarovii
Key words: embryo and endosperm formation, micro- and macrosporogenesis, monitoring,
endemics

ANATOMICAL AND MICROMORPHOLOGICAL

INVESTIGATIONS OF ARTEMISIA ARBORESCENS
L. - WILD-GROWING MEDICINAL PLANT FROM
MONTENEGRO
3HGMD-DQDüNRYLü10LODQ*DYULORYLü1'UDJDQD5DQþLü2=RUD'DMLü
6WHYDQRYLü2'DQLMHOD6WHãHYLü3, Petar D. Marin1

University of Belgrade - Faculty of Biology, Institute of Botany and Botanical Garden
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2
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3
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,Q WKLV ZRUN DQDWRPLFDO DQG PLFURPRUSKRORJLFDO LQYHVWLJDWLRQV RI YHJHWDWLYH RUJDQV RI
Artemisia arborescens / &RPSRVLWDH ZLOGJURZLQJ PHGLFLQDO SODQW IURP 0RQWHQHJUR
ZHUHFRQGXFWHG7KHPDLQJRDOZDVWRH[DPLQHWKHDQDWRP\DQGSDUWLFXODUDQDWRPLFDODQG
PLFURPRUSKRORJLFDO FKDUDFWHULVWLFV ZKLFK DUH LQ UHODWLRQ ZLWK SURGXFWLRQ RI VSHFLDOL]HG
PHWDEROLWHV0LFURVFRSLFVOLGHVZHUHSUHSDUHGIROORZLQJWKHVWDQGDUGKLVWRORJLFDOSURFHGXUHV
Triarch type of the vascular bundle is present in primary root structure, whereas typical
VHFRQGDU\ JURZWK RFFXUV LQ ROGHU URRW 7KH VWHP LV FKDUDFWHUL]HG E\ FROODWHUDO YDVFXODU
EXQGOHVVHSDUDWHGE\ZLGHSDUHQFK\PDUD\V7KHODUJHVWSDUHQFK\PDFHOOVRFFXULQWKHSLWK
In the older stem, below the epidermis, well developed hypodermis could be noticed, made
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DUUDQJHGE\FROOHQFK\PDDQGFKORUHQFK\PDDQGRQHFHQWUDODQGWZRODWHUDOYDVFXODUEXQGOHV
&RQFHUQLQJOHDIDQDWRP\WKHLVRODWHUDOSDOLVDGHVWUXFWXUHDQG.UDQ]DQDWRP\&SK\VLRORJ\
DUHREVHUYHG6HFUHWRU\GXFWVDUHSUHVHQWLQWKHVWHPFRUWLFDOSDUHQFK\PDDQGLQWKHOHDIDQG
SHWLROHSDUHQFK\PD1XPHURXVPRUSKRORJLFDOO\YDULDEOH7VKDSHGQRQJODQGXODUDVZHOODV
JODQGXODUWULFKRPHVRFFXURQWKHVXUIDFHRIDOODHULDOYHJHWDWLYHRUJDQV(DUOLHUKLVWRFKHPLFDO
FKDUDFWHUL]DWLRQ RI WKH ROHRUHVLQ SURGXFHG E\ VHFUHWRU\ GXFWV RI VRPH Artemisia species
VKRZHGWKHSUHVHQFHRIWHUSHQRLGVHVVHQWLDORLOVDONDORLGVIDWW\DFLGVDQGSRO\DFHW\OHQHV
ZKLOHLQWKHJODQGXODUWULFKRPHVVHVTXLWHUSHQHODFWRQHVVWHURLGVDQGWDQQLQVDUHUHYHDOHGLQ
DGGLWLRQWRHVVHQWLDORLOVZKHUHDOORIWKHPDUHNQRZQIRUWKHLUPHGLFDOSURSHUWLHV7KXVRXU
¿QGLQJVDUHRIDQLPSRUWDQFHIRUIXWXUHKLVWRFKHPLFDODQGSK\WRFKHPLFDOLQYHVWLJDWLRQVRIWKLV
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Key words: anatomy, micromorphology, Artemisia

PLANTS TRADITIONALLY USED AS ANTITUSSIVES IN

TURKEY
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7XUNH\
5HVSLUDWRU\GLVRUGHUVDUHWKHPRVWFRPPRQGLVHDVHVLQZRUOGZLGH&RXJKDV\PSWRPRIPRVW
respiratory illnesses, is a physiological mechanism that clears the airways and is also treated
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WKHUDSHXWLF DUVHQDO IRU KHDOHUV LQ$IULFD DQG WKH PRVW RI GHYHORSLQJ FRXQWULHV 'HVSLWH WKH
GHYHORSPHQWRIDYDULHW\RIGUXJVFRXJKLVDGLI¿FXOWGLVHDVHWREHHUDGLFDWHG(WKQRERWDQLFDO
studies carried out by traditional methods of treatment are recorded and this information is
DLPHGWRFRQWULEXWHWRWKHGHYHORSPHQWRIWKHGUXJ7KHLQIRUPDWLRQDERXWKRZWRWUDGLWLRQDOO\
XVHWKHVHPHGLFLQDORUZLOGSODQWVKDVEHHQWUDQVIHUUHGIURPJHQHUDWLRQWRJHQHUDWLRQ,QWKLV
study, ethnobotanical studies have been conducted and found a lot of taxa used in traditional
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Key words:FRXJKPHGLFLQDOSODQWVWUDGLWLRQDOWUHDWPHQW7XUNH\

MEDICINAL PLANTS IN FOLK TRADITION: A

PRELIMINARY ETHNOBOTANICAL STUDY OF
3h/h0h5781&(/,785.(<
Ahmet Dogan
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6WUHHW1R,VWDQEXO7XUNH\
7KHJRDORIWKLVVWXG\LVWRLGHQWLI\WKHSODQWVXVHGDVWUDGLWLRQDOIRONPHGLFLQHLQ3OPU
)RUWKLVSXUSRVHWKH¿HOGZRUNZDVGRQHEHWZHHQ$SULO1RYHPEHU'XULQJWKLV
UHVHDUFKDOOYLOODJHVRI3OPUGLVWULFWZHUHYLVLWHG7KHVSHFLPHQVRIWKHSODQWVXVHGDVIRON
remedies, have been collected and the information about the local names, the part(s) used, the
ailments treated, the therapeutic effect, the preparation, the methods of administration and
the duration of treatment were obtained from the native people living in the research area by
SHUVRQDOLQWHUYLHZV7KHSODQWVSHFLPHQVZKLFKDUHWKHPDWHULDOVRIWKLVVWXG\ZHUHLGHQWL¿HG
XVLQJWKH³)ORUDRI7XUNH\DQGWKH(DVW$HJHDQ,VODQGV´DQGWKH\DUHNHSWLQWKH+HUEDULXPRI
WKH)DFXOW\RI3KDUPDF\0DUPDUD8QLYHUVLW\0$5($QHWKQRERWDQLFDODQDO\VLVVKRZHG
WKDWSODQWWD[DDUHXVHGDVDWUDGLWLRQDOIRONPHGLFLQHLQ3OPU$FFRUGLQJWRWKHPDMRULW\
of the plants which have similar usage, the plants are mostly used for treatment of wounds,
UHVSLUDWRU\GLJHVWLYHDQGFLUFXODWRU\V\VWHPGLVHDVHV
Key words:7XQFHOL7XUNH\IRONPHGLFLQDOSODQWVHWKQRERWDQ\

FEVERFEW TANACETUM PARTHENIUM (L.) Sch. Bip. –

NATIVE SPECIES FROM SOUTH EAST EUROPE USED
IN LITHUANIAN AND BELORUSSIAN TRADITIONAL
MEDICINE
/DLPDâYHLVW\Wơ1, Alesia Kruchonok2.ULVWLQD/RåLHQơ3

3ODQW*HQH%DQN/LWKXDQLD6WRWLHVVWU/7$NDGHPLMD/LWKXDQLD
2
Central Botanical Garden of the National Academy of Sciences of Belarus,
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3
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7KHULFKDQGGLYHUVHWUDGLWLRQVRIPHGLFLQDOSODQWVLQ/LWKXDQLDDQG%HODUXVFRXQWULHVDUHWKH
UHVXOWRIWKHHWKQLFDOO\PL[HGSRSXODWLRQVRIWKLVUHJLRQ,QYHVWLJDWLRQVLQWRPHGLFLQDOSODQWV
LQ/LWKXDQLDZHUHFRPPHQFHGLQWKHthFHQWXU\8QWLOWKHQ&DWKROLFPRQDVWHULHVSOD\HGD
VLJQL¿FDQW SDUW LQ VSUHDGLQJ SK\WRWKHUDSKHXWLF SUDFWLVHV ,Q /LWKXDQLD DQG %HODUXV D ÀRZHU
JDUGHQXVHGWREHDGHFRUDWLYHSDUWRIWKHKRPHVWHDG,QIRUPHUWLPHVERWKZLOGDQGFXOWLYDWHG
plants from the garden were used in everyday life too, many plants were used to cure various
LOOQHVV 2QH RI WKHP IHYHUIHZ Tanacetum parthenium / 6FK%LS. QDWLYH WR %DONDQ¶V
3HQLQVXODKDVDORQJKLVWRU\RIXVHDQGJURZLQJQHDUKRPHVWHDG,WZDVPHQWLRQHGLQth
FHQWXU\7KHPDLQREMHFWLYHVRIWKLVVWXG\ZHUHWRFROOHFWLQIRUPDWLRQRQWKHPHGLFLQDOXVHV
RIIHYHUIHZLQSDVWDQGQRZDGD\V7KHSODQWZDVZLGHO\JURZLQWKHHDUO\RIWKHth century
WKURXJKRXW/LWKXDQLDDQG%HODUXVIRUPHGLFLQDODQGRUQDPHQWDOSXUSRVHV7KHIHYHUIHZZDV
LPSRUWDQWIRUWUHDWPHQWZRPHQ¶VLOOQHVVHVVXSSUHVVLQJLQÀDPPDWLRQVSDVPVDQGKHDGDFKHV
GLJHVWLYH V\VWHP GLVRUGHUV DV D VHGDWLYH 1RZDGD\V WKH WUDGLWLRQDO NQRZOHGJH FRQFHUQLQJ
XVH RI IHYHUIHZ UHPDLQV LQ WKH PHPRULHV RI ROGHU SHRSOH 7KLV SODQW FDQ EH IRXQG LQ ROG
KRPHVWHDGV HVSHFLDOO\ LQ WKH IRUHVWHG SDUW RI 6RXWK (DVWHUQ /LWKXDQLDQ DQG 1RUG (DVWHUQ
%HODUXV
Key words: feverfew, use, traditional medicine

SIDERITIS RAESERI Boiss. & Heldr. (ÇAJI I MALIT)

DISTRIBUTION IN ALBANIA, CHALLENGES FOR THE
FUTURE
Alban Ibraliu1, Belul Gixhari2, Najada Kadiasi1, Arlinda Agastra1, Rea
Tako1, Jonida Hysko1

Department of Plant Sciences and Technologies, Agricultural University of
Tirana, Tirana, Albania
2
$OEDQLDQ*HQHEDQN$JULFXOWXUDO8QLYHUVLW\RI7LUDQD7LUDQD$OEDQLD
Sideritis raeseri %RLVVHW +HOGU dDML L 0DOLW LV LQFOXGHG LQ WKH 5HG %RRN RI$OEDQLD DV
DQHQGDQJHUHGVSHFLHVJURZLQJZLOGLQ$OEDQLD7KHDLPRIWKLVUHVHDUFKZDVWRLQYHVWLJDWH
the natural distribution areas of Sideritis raeseri and to identify the possible cultivation
areas assessing the relationships between environmental conditions and the physiological
and morphometric characteristics of Sideritis raeseri SRSXODWLRQV %\ LGHQWLI\LQJ WKH PRVW
important natural growing areas of Sideritis raeseri populations, this study will contribue in
WKHLQFUHDVLQJRIWKHUHSUHVHQWDWLYHQHVVRIdDMLL0DOLW germplasm LQJHQHEDQNGDWDEDVHDQG
WKHSRVVLEOHTXDQWLW\RILQFRPHVIRUWKHORFDOSHRSOHDVWKHORFDOHPSOR\PHQWVDUHFRQQHFWHG
to an increasment of SideritisSRSXODWLRQVLQWKHPRVWQDWXUDOJURZLQJDUHDVRIWKLVVSHFLHV
'HWHUPLQLQJWKHPDSLPSDFWRIFXOWLYDWLRQRIdDMLL0DOLWQDWXUDOSRSXODWLRQVLVDQLPSRUWDQW
XQGHUWDNLQJHVSHFLDOO\LQVXVWDLQDELOLW\RIWKHIDPLOLHVDQGLQGLYLGXDOVVLWXDWHGRQWKHQDWXUDO
growing areas of SideritisSRSXODWLRQV)LQDOO\WKHVWXG\KDVFRQWULEXWHGLQWKHGHYHORSPHQW
RIdDMLL0DOLWGDWDEDVHFRYHULQJWKHJHRJUDSKLFQDWXUDOGLVWULEXWLRQRISideritis raeseri and
VSDUVHO\FXOWLYDWLRQDUHDVQHDURI1DWXUDO3DUNVLQ$OEDQLD
Key words: Sideriti raeseri, Albania, database

IN SITU CONSERVATION OF THREE ENDEMIC

VERBASCUM SPECIES IN BULGARIA BY POPULATION
REINFORCEMENT
Marina Stanilova, Svetlana Bancheva, Malina Delcheva, Stoyan
Stoyanov, Boriana Sidjimova, Boryanka Traykova
Genus Verbascum 6FURSKXODULDFHDH FRPSULVHV DERXW VSHFLHV VRPH RI WKHP XVHG LQ
IRONPHGLFLQHDVWHDRLQWPHQWEDWKVKRZHYHUPDQ\VSHFLHVDUHHQGHPLFDQGXQH[SORUHGIRU
WKHLUELRDFWLYHFRPSRXQGVVerbascum tzar-borisii'DYɟ[6WRM6WHI*DWDQGVerbascum
GDYLGRI¿L 0XUE DUH %XOJDULDQ HQGHPLFV WKH ¿UVW RQH ZLWK WZR SRSXODWLRQV RI DERXW
LQGLYLGXDOVWKHODWWHUZLWKDXQLTXHVHYHUHO\IUDJPHQWHGSRSXODWLRQVerbascum anisophyllum
0XUELVD%DONDQHQGHPLFZLWKWZRSRSXODWLRQVLQ%XOJDULDDQGRQHLQ6HUELD7KHVHVSHFLHV
DUHLQFOXGHGLQWKH5HG'DWD%RRNRI%XOJDULDDV³&ULWLFDOO\(QGDQJHUHG´DQGSURWHFWHGE\
WKH%LRGLYHUVLW\$FW7KHLQYHVWLJDWLRQGHDOVZLWKin situ conservation of the target species,
based on monitoring of their populations, seed germination rate, and in vitroSODQWSURSDJDWLRQ
,Q QDWXUH DOO WKUHH VSHFLHV DUH FKDUDFWHUL]HG ZLWK XQVWDEOH SURSRUWLRQV EHWZHHQ YHJHWDWLYH
URVHWWHVDQGJHQHUDWLYHÀRZHULQJLQGLYLGXDOVYDU\LQJIURP\HDUWR\HDU2EVHUYDWLRQVLQWKH
ex situ collection of IBER proved V. anisophyllum to be monocarp, while plants of the perennial
V. tzar-borisii UHDFKHG ÀRZHULQJ VWDJH RQ WKHLU VHFRQG \HDU DQG EORRPHG IRXU FRQVHFXWLYH
\HDUV LQ WKH JUHHQKRXVH WZLFH \HDUO\ IRUPLQJ VHYHUDO ÀRZHU VWDONV 6HHG JHUPLQDWLRQ UDWH
ZDVVLJQL¿FDQWO\LQFUHDVHGin vitroE\VHHGVRDNLQJLQJLEEHUHOOLFDFLGSULRUWRFXOWLYDWLRQRQ
06PHGLXPIURPWRIRU9GDYLGRI¿LIURPWRIRUV. anisophyllum, and from
WRIRUV. tzar-borisii3ODQWVZHUHex vitro adapted in a phytotron, and after wintering
LQWKHXQKHDWHGJUHHQKRXVHWKH\ZHUHSODQWHGLQWKHLUSRSXODWLRQVRIRULJLQV. tzar-borisii
SODQWVLQ0DUFKV. anisophyllum, and 25 9GDYLGRI¿LSODQWVLQ-XQH0RQLWRULQJ
LQ0D\FRQ¿UPHGWKHVXFFHVVIXODFFOLPDWL]DWLRQRIV. tzar-borisii plants (28 rosettes
DQGÀRZHULQJ0RQLWRULQJRIWKHSODQWVIURPWKHWZRRWKHUVSHFLHVLVIRUHVHHQIRU-XQH
Key words: mullein, in vitro seed germination, monitoring

PLANTS USED IN TRADITIONAL ALCOHOLIC

BEVERAGES OF THE ADRIATIC ISLANDS (CROATIA)
,YDQD9LWDVRYLü.RVLü10DULMD-XJ'XMDNRYLü2, Katija Dolina3, Mirjana
-HULþHYLü4àXNDV]àXF]DM5

University of Zagreb, Faculty of Agriculture, Department of Agricultural Botany,
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to the south of the Adriatic, are: Myrtus communis / LVODQGV IROORZHG E\
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WUDGLWLRQ GLVWLOOHG LQWR JUDSSD EDVHG RQ Vitis vinifera / UDNLMD OR]D DGGHG DIWHU WKH
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and ArtemisiaRUVRPHRWKHUVSHFLHVFDQEHXVHGDVWKHEDVHIRUUDNLMDHJArbutus, Ficus
carica/HWF7KHDERYHPHQWLRQHG7$%VDUHXVHGLQHYHU\GD\OLIHIRULQVWDQFHDV
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Key words:HWKQRERWDQ\$GULDWLFLVODQGVUDNLMDOLTXHXUWUDGLWLRQDODOFRKROLFEHYHUDJH
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MEDICINAL PLANTS IN THE INTENSIVELY MANAGED

AGRICULTURAL LANDSCAPE IN PAZARDZHIK -
PLOVDIV REGION OF BULGARIA
Ina Aneva1, Ekaterina Kozuharova2, Peter Zhelev3, Simeon Lukanov1,
Maria Peneva4

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The objective of present study was to assess the diversity of medicinal plants in the agricultural
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use were included in the study ranging from cereal crops (the predominant type) through
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was recorded in the remnants of woody vegetation (Solitary trees and small groups of trees
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XVLQJRISHVWLFLGHVSRVVHVVDVHULRXVREVWDFOHWRWKHXVHRIWKHVHVSHFLHVIRUPHGLFLQDOSXUSRVHV
Key words: medicinal plants, land use, agriculture
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Project “BIOGEA”

EFFECT OF ARBUSCULAR MYCORRHIZAE ON

PHOSPHORUS DEFICIT STRESS DURING EARLY
DEVELOPMENT STAGE OF BASIL (OCIMUM
BASILICUM L.)
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Key words:EDVLODUEXVFXODUP\FRUUKL]DHURRWWUDLWVFKORURSK\OOÀXRUHVFHQFHPLQHUDO
content

GENUS INULA L. IN BULGARIA – DISTRIBUTION AND

NATURAL HABITATS
Peter Zhelev1, Ina Aneva2$QWRDQHWD7UHQGD¿ORYD3, Milka Todorova3

2
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2
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Genus Inula LV UHSUHVHQWHG E\ VSHFLHV LQ %XOJDULD 0RVW RI WKHP DUH ZLGHO\ GLVWULEXWHG
DFURVVWKHFRXQWU\DQGWKUHHVSHFLHVDUHFRQVLGHUHGUDUH2QHVSHFLHV± I. spiraeifolia/LV
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group includes species occurring on limestone in open places (for example, I. oculus-christi
/ DQG I. aschersoniana -DQND )UHTXHQWO\ WKH\ DUH SDUW RI WKH KDELWDWV $ (DVWHUQ VXE
PHGLWHUUDQHDQGU\JUDVVODQGV&DOFDUHRXVURFN\VORSHVZLWKFKDVPRSK\WLFYHJHWDWLRQDQG
$UERUHVFHQWPDWRUUDOZLWKJuniperusVSS7KHVHFRQGJURXSFRQVLVWVRIVSHFLHVZKLFKDUH
found mostly on calcareous soils but occur also under wide range of ecological conditions (for
example, I. bifrons//I. conyza/7KHWKLUGJURXSLQFOXGHVRQO\RQHVSHFLHV±I. ensifolia
/ZKLFKLVIRXQGH[FOXVLYHO\LQIRUHVWKDELWDWVPRVWO\6XE0HGLWHUUDQHDQSLQHIRUHVWV
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species can also be found in the forests, especially in the peripheral parts (for example, I.
helenium / 7KH IRXUWK JURXS LQFOXGHV WKH PRVW WROHUDQW VSHFLHV RFFXUULQJ LQ YDULHW\ RI
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Key words: Inula, Asteraceae, medicinal plants, habitats
Acknowledgement: This work was supported by the NSF, Ministry of Education and Science,
Bulgaria, Project DN 09/11.

COMPARATIVE ANATOMY, MICROMORPHOLOGY

AND BIOCHEMISTRY OF SAMBUCUS NIGRA L. FRUITS
1DHOD&RVWLFă1,2$QL‫܈‬RDUD6WUDWX1, Irina Boz3, Elvira Gille4

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Integrated Centre for Environmental Science Studies in the North-East Development Region
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Department of Experimental and Applied Biology, NIRDBS-Institute of Biological Research
,DVL5RPDQLDG1,5'%6´6WHMDUXO´%LRORJLFDO5HVHDUFK&HQWUH3LDWUD1HDPW5RPDQLD
Sambucus nigra/LVDSRSXODURUQDPHQWDOVKUXEDQGDKLJKO\YDOXHGPHGLFLQDOSODQWDVLWLV
DVRXUFHRIPDQ\ELRDFWLYHFRPSRXQGV,WVÀRZHUVDQGIUXLWVDUHZLGHO\XVHGLQWKH5RPDQLDQ
WUDGLWLRQDOPHGLFLQH)UXLWRISambucus nigra is of increasing commercial interest for juice,
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of Sambucus nigra fruits and the accumulation of bioactive substances in these organs, is
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physico-biochemical features of Sambucus nigraIUXLWVKDUYHVWHGIURP¿YHGLIIHUHQWORFDWLRQV
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RIK\SRGHUPDOFHOOVDQGRIPHVRFDUSFHOOVWKHWKLFNQHVVRIPHVRFDUSSDUHQFK\PDOD\HUV7KH
micromorphology of the epicarp showed the aspect of cuticle, with numerous parallel oriented
crystalline wax platelets, as well as spherical deposits of wax alternating with smooth areas
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IURP WKH IUXLWV MXLFH FRQWHQWV LQ ZDWHU GU\ PDWWHU DQG WRWDO PLQHUDO HOHPHQWV 7KH DERYH
VSHFL¿HG SDUDPHWHUV LQGLFDWH VSHFL¿F YDULDWLRQV UHODWHG WR WKH IUXLWV VRXUFH ORFDWLRQ D KLJK
content in water and solid matter, a moderate content in mineral elements, a strong negative
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Key words: Sambucus nigra, structure, epicarp, mesocarp, micromorphology, biochemistry
Acknowledgement: The authors wish to thank the BIODIVERS 2 National Project (PN 16-
190401) for the funding.

MEDICINAL BOTANICAL KNOWLEDGE AMONG THE

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includes Achillea millefolium /., Cornus mas, Hypericum perforatum /., Juglans regia /.,
Matricaria chamomilla, MenthaVSSOriganum vulgare /Plantago major /., ThymusVSS
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ASSESSMENT OF POPULATION DIVERSITY IN WILD

RUBUS IDAEUS L. POPULATIONS FROM SERBIA
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SODQWNLQJGRP5DVSEHUU\Rubus idaeus/LVGLSORLGDQGEHORQJVWRVXEJHQXV,GDHREDWXV
Raspberry leaves are used in traditional medicine for treatment of various disorders most
commonly related to menstruation, parturition, and ailments of the gastrointestinal tract and
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intrapopulation diversity and interpopulation relationships of seven wild growing populations
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the samples into three clusters of which to cluster C belong exclusively individuals originating
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THE TREATMENT OF MENORRHAGIA WITH

BIOAPIGYN® HERBAL OINTMENT AND PESSARIES
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for the topical treatment of menorrhagia using macerates and essential oils of medicinal
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Alchemilla vulgaris/, Achilea millefolium/, Plantago major/, Polygonum aviculare/,
6DOYLDRI¿FLQDOLV/, Quercus robur/, Olea europaea/&DOHQGXODRI¿FLQDOLV/, Matricaria
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caryophyllata, Cympobogon martini, Cinnamomum camphora FW FLQHRO, Thymus vulgaris
FWWLPRO, Origanum compactum.2WKHULQJUHGLHQWVLQFOXGHGKRQH\JO\FHUROCera alba and
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XVHGSHVVDULHVLQVWHDG7KHUHVXOWVZHUHREWDLQHGDWEDVHOLQHDQGIROORZLQJWKHWKLUGPHQVWUXDO
F\FOH7KHLPSURYHPHQWZDVDFFRPSDQLHGE\UHGXFWLRQLQWKHQXPEHURIVSHQWVDQLWDU\SDGV
RUWDPSRQVZDNLQJXSGXULQJWKHQLJKWLQRUGHUWRFKDQJHWKHSDGVRUWDPSRQVWKHGXUDWLRQ
RIEOHHGLQJDSSHDUDQFHRIWKHEORRGFORWVDQGWKHQXPEHURISDLQNLOOHUVFRQVXPHG,QERWK
JURXSVDVLJQL¿FDQWGHFUHDVHZDVREVHUYHGIRUDOOWKHV\PSWRPVS6LJQL¿FDQWO\EHWWHU
results were obtained in case of the ointment compared to the pessaries as expected due to the
KLJKHUSHUFHQWDJHRIWKHDFWLYHLQJUHGLHQWV7KHDYHUDJHGXUDWLRQRIEOHHGLQJLVUHGXFHGIURP
WRGD\VLQWKHFDVHRIWKHRLQWPHQWDQGIURPWRGD\VLQWKHJURXSZKRXVHG
SHVVDULHV7KHDYHUDJHQXPEHURISDLQNLOOHUV LVUHGXFHGIURPWRDQGIURPWR
LQWKHJURXSXVLQJWKHRLQWPHQWDQGSHVVDULHVUHVSHFWLYHO\'HFUHDVHLQRWKHUV\PSWRPV
UDQJHGIURPXSWRLQWKHJURXSXVLQJWKHRLQWPHQWDQGIURPWRLQ
WKHFDVHRISHVVDULHVXVDJH7KRVHUHVXOWVFRXOGEHFRUUHODWHGZLWKWKHLQJUHGLHQWVZLWKSURYHQ
KDHPRVWDWLFYDVRFRQVWULFWRUDVWULQJHQWDQGVSDVPROLWLFDFWLYLW\
Key words: olive oil macerates, medicinal plants, essential oils, menorrhagia, herbal ointment,
pessaries

EVALUATION OF THE DIVERSITY OF COMMON

JUNIPER (JUNIPERUS COMMUNIS L.) FROM THE
TYPICAL GEOGRAPHIC AREAS IN ALBANIA
Arlinda Agastra1, Belu Gixhari2, Alban Ibraliu1

Department of Plant Sciences and Technologies, Agricultural University of Tirana,
Tirana, Albania
2
$OEDQLDQ*HQHEDQN$JULFXOWXUDO8QLYHUVLW\RI7LUDQD7LUDQD$OEDQLD
Juniperus communis /DZLOGVKUXEVSHFLHVJURZLQJHVSHFLDOO\LQPRXQWDLQDUHDVRI$OEDQLD

LQDVVRFLDWLRQZLWKWKHRWKHUPRVWLPSRUWDQWFRQLIHURXVVSHFLHV,WLVLQFOXGHGLQWKH5HG%RRN
RI$OEDQLDDVDQHQGDQJHUHGVSHFLHVJURZLQJZLOGLQ$OEDQLD1RZDGD\VEHUULHVRI-XQLSHUXV
are exported from Albania to many countries and especially in EU countries and are considered
as the important income source for the local people that live in the poor mountain areas of the
FRXQWU\$VVHVVPHQWRIJHRJUDSKLFGLVWULEXWLRQRIVHYHUDOSRSXODWLRQVRI-XQLSHUXVREVHUYHG
LQGLVWULFWVRI$OEDQLD0DOHVLH0DGKH7URSRMH.XNHV'LEHU(OEDVDQ/LEUD]KG%LOLVKW
.RUFH(UVHNH/HVNRYLNZDVFDUULHGRXW%HUU\IUXLWVZHUHDOVRFROOHFWHGIURPGLIIHUHQW
QDWXUDOJURZLQJDUHDVRI-XQLSHUXVLQ$OEDQLD6SDWLDODQDO\VLVXVLQJJULGVTXDUHFHOOVRI[
NPDQG[NPGHWHFWVWKHDUHDVRIKLJKGLYHUVLW\RI-XQLSHUXVSRSXODWLRQV7KHJHRJUDSKLF
DUHDVZHUHVHSDUDWHGLQWRVPDOOJULGVTXDUHFHOOVDQGJULGFHOOVRI[NPDQG[NP
was used to assess the geographic distribution, diversity indices, and richness estimators of
RUHJDQRSRSXODWLRQV7KHELRGLYHUVLW\PRQLWRULQJRI-XQLSHUSRSXODWLRQVJURZLQJQDWXUDOO\LQ
GLIIHUHQWPRXQWDLQDUHDVRI$OEDQLDFRQWULEXWHVWRWKHLQFUHDVLQJHI¿FLHQF\DQGHQKDQFHPHQW
RIEHYHUDJHVDQGIRRGLQGXVWU\
Key words: Juniperus communis, geographic distribution, Albania

MORPHOLOGICAL, AGRONOMIC AND GENETIC

CHARACTERIZATION OF RHODIOLA ROSEA L.
ACCESSIONS FROM THE PROVINCE OF TRENTO,
AS PRELIMINARY RESULTS FOR A STUDY ON THE
BIODIVERSITY OF THE SPECIES
Pietro Fusani1, Nicola Aiello1, Luisa Palmieri2, Stefano Piazza2, Stefan
Martens2, Fabrizio Scartezzini1

Research Centre for Forestry and Wood, Council for Agricultural Research and Economics
&5($SLD]]D1LFROLQLORF9LOOD]]DQR7UHQWR,WDOLD
2
5HVHDUFKDQG,QQRYDWLRQ&HQWUH(GPXQG0DFK)RXQGDWLRQ9LD(0DFK6DQ
0LFKHOHDOO¶$GLJH71,WDO\
Rhodiola rosea/LVDGLRHFLXVSHUHQQLDOSODQWEHORQJLQJWRWKHIDPLO\RI&UDVVXODFHDHZKLFK
JURZVLQ(XURSH$VLD DQG1RUWK$PHULFD,Q (XURSHLWVGLVWULEXWLRQDUHDHQFRPSDVVHVWKH
1RUWKRI(XURSHDQGPRVWRIWKHPRXQWDLQVRI&HQWUDO(XURSHLQFOXGLQJWKHHQWLUH$OSLQH$UF
,WV XQGHUJURXQG SDUWV UKL]RPHV DQG URRWV FRQWDLQ DFWLYH FRPSRXQGV ZLWK ZHOO HVWDEOLVKHG
DGDSWRJHQLFSURSHUWLHVZKLFKPDNHLWDZRUOGZLGHNQRZQPHGLFLQDOSODQW$WWKHPRPHQWWKH
PDUNHWGHPDQGRIUDZPDWHULDOLVPRVWO\VXSSOLHGE\WKHZLOGFROOHFWLRQDQGLWVFXOWLYDWLRQLV
EHFRPLQJLQFUHDVLQJO\QHFHVVDU\GXHWRWKHULVNRIHQGDQJHULQJLWVORQJWHUPVXUYLYDOLQWKH
areas in which it is collected, but just a few cultivated varieties of the species are available
RQ WKH PDUNHW 7KH DLP RI WKLV VWXG\ ZDV WR HYDOXDWH WKH PRUSKRORJLFDO DQG DJURQRPLF
characteristics and the genetic variability of eight accessions of the species originating from as
many wild populations collected in the province of Trento, North east of Italy, and cultivated
ex situ3ODQWOHWVRIWKHYDULRXVDFFHVVLRQVZHUHREWDLQHGIURPVHHGVDQGWUDQVSODQWHGLQDQ
H[SHULPHQWDOWULDODWPDVOLQWKHVXPPHURI0RUSKRORJLFDOWUDLWVZHUHUHFRUGHG
IURP WKH WKLUG WR WKH ¿IWK \HDU RI FXOWLYDWLRQ E\ WKH XVH RI GHVFULSWRUV OLVW IRU WKH VSHFLHV
HVSHFLDOO\PRGL¿HGIRUWKLVVWXG\DQG\LHOGVRIURRWVDQGUKL]RPHVZHUHUHFRUGHGLQWKHIRXUWK
DQG¿IWK\HDUIURPWUDQVSODQW)LQDOO\WKHSODQWOHWVEHORQJLQJWRWKHVHOHFWHGDFFHVVLRQVZHUH
JHQRW\SHGXVLQJSXEOLVKHG665Simple sequence repeatPDUNHUV$JUHDWYDULDELOLW\LQ
morphological traits, genetics and yields was observed among investigated accessions and this
FRXOGMXVWLI\LIVXSSRUWHGE\WKHFXUUHQWO\XQGHUZD\TXDOLWDWLYHFKDUDFWHUL]DWLRQRIWKHVDPH
DFFHVVLRQVWKHLPSURYHPHQWRIWKHVSHFLHVE\DEUHHGLQJSURJUDP
Key words: Rhodiola rosea PRUSKRORJLFDO FKDUDFWHUL]DWLRQ DJURQRPLF FKDUDFWHUL]DWLRQ

JHQHWLFFKDUDFWHUL]DWLRQ

ESSENTIAL OIL EXTRACT, DETERMINATION

OF NANO-EMULSIONS CHARACTERISTICS AND
ALLELOPATHIC POTENTIAL OF MEDICINAL PLANT
DUCROSIA ANETHIFOLIA (DC.) Boiss
Saifullah Naikandish
6KLUD]8QLYHUVLW\,UDQ
This research aims to identify and determine the chemical composition of the essential oil
(2 FKDUDFWHULVWLFV RI (2 QDQRHPXOVLRQ DOOHORSDWKLF HIIHFWV RI (2 DQG DTXHRXV H[WUDFW
of the aerial parts of Ducrosia anethifolia'&%RLVV7KHSODQWPDWHULDOZDVVXEMHFWHGWR
K\GURGLVWLOODWLRQ E\ XVLQJ &OHYHQJHU DSSDUDWXV DQG WKH REWDLQHG (2 ZDV DQDO\]HG E\ *&
DQG*&06LQVWUXPHQW7KHPDMRUFRPSRXQGVRI(2ZHUHQGHFDQDOGRGHFDQDO
QGHFDQRO (GRGHFDQDO DQG DOSKDSHQLQH 7KH
(2 QDQRHPXOVLRQV ZHUH PDGH E\ WKH VWDELOL]HU VROXWLRQV RI SRO\YLQ\O DOFRKRO 39$ DQG
SRO\YLQ\OS\UUROLGRQH3937KHFKDUDFWHULVWLFVRIWKHQDQRHPXOVLRQLQFOXGLQJGHQVLW\S+
electrical potential, total dissolved solids (TDS), electrical conductivity, electrical resistance,
]HWD SRWHQWLDO SDUWLFOH VL]H YLVFRVLW\ DQG DQWLR[LGDQW ZHUH LQYHVWLJDWHG DQG FRPSDUHG WR
WKH FRQWURO 7KH UHVXOWV VKRZHG WKDW GHQVLW\ S+ HOHFWULFDO UHVLVWDQFH ]HWD SRWHQWLDO DQG
SDUWLFOHVL]HGHFUHDVHGZKLOHHOHFWULFDOSRWHQWLDO7'6HOHFWULFDOFRQGXFWLYLW\YLVFRVLW\DQG
DQWLR[LGDQWDFWLYLW\RIQDQRHPXOVLRQ(2LQFUHDVHG7KHHIIHFWVRIDTXHRXVH[WUDFWVRQVHHG
germination and growth of Lepidium sativum / DQG $PDUDQWKXV UHWURÀH[XV / at different
concentrations DQGWKHHIIHFWVRI(2RQVHHGVRI$UHWURÀH[XV
DWWKHFRQFHQWUDWLRQVRIDQG//, and on the seeds of L. sativum
DWWKHFRQFHQWUDWLRQRIDQG//ZHUHLQYHVWLJDWHG7KH
measured characteristics included: germination percentage, plumule length, radical length,
JHUPLQDWLRQVSHHGDOOHORSDWKLFLQGH[VHHGVYLJRULQGH[DQGDOORPHWULFFRHI¿FLHQW. The results
VKRZHGWKDWWKHDTXHRXVH[WUDFWKDGWKHKLJKHVWLQKLELWLRQHIIHFWRQWKHPHDVXUHGSURSHUWLHV
of A. UHWURÀH[XVand L. sativumDWWKHFRQFHQWUDWLRQVRIDQGJHUPLQDWLRQUHDFKHG
]HUR UHVSHFWLYHO\ 7KH D anethifolia (2 KDG VLJQL¿FDQW LQKLELWLRQ HIIHFW RQ WKH PHDVXUHG
properties of $UHWURÀH[XVDWWKHFRQFHQWUDWLRQRI//JHUPLQDWLRQUHDFKHG]HUR7KH
HIIHFWRI(2ZDVVLJQL¿FDQWRQWKHIROORZLQJSDUDPHWHUVJHUPLQDWLRQVSHHGVHHGYLJRULQGH[
DOORPHWULFFRHI¿FLHQWUDGLFDOOHQJWKDQGSOXPXOHOHQJWKRIL. sativum seedsKRZHYHULWGLG
QRWDIIHFWWKHJHUPLQDWLRQSHUFHQWDJHDQGDOOHORSDWKLFLQGH[
Keyword: Ducrosia anethifolia, essential oil, extract, antioxidant, phenolic compound, poly
vinyl alcohol, polyvinyl pyrrolidone, allelopathic activity

POPULATION STATUS AND HABITATS OF ALLIUM

URSINUM L. IN BULGARIA
Ina Aneva1, Milena Nikolova1, Peter Zhelev2, Katya Georgieva1

2
Allium ursinum/LVDYDOXDEOHPHGLFLQDOSODQWXVHGDOVRIRUIRRG:HSUHVHQWUHVXOWVRIDVWXG\
RQSODQWFRPPXQLWLHVDQGKDELWDWVRIUHSUHVHQWDWLYHORFDOLWLHVLQ%XOJDULD7KHVSHFLHVRFFXUV
LQÀRULVWLFUHJLRQVDQGPRVWORFDOLWLHVDUHLQPHVRSK\WLFIRUHVWVWDQGV)ORULVWLFFRPSRVLWLRQ
FRQVLVWVRIUHODWLYHO\VPDOOQXPEHURIVSHFLHVW\SLFDOIRUWKHIRUHVWKDELWDWV3RSXODWLRQVWDWXV
of the species is stable, and in many places, it is subjected to collection with economic purpose
DQGE\WKHORFDOSHRSOH7KHPRVWFRQVHUYHGDQGQXPHURXVSRSXODWLRQVZHUHHVWDEOLVKHGLQ
6WDUDSODQLQD0WVDQGWKHVFDUFHVWSRSXODWLRQVZHUHIRXQGLQ/XOLQ0WV9LWRVKDUHJLRQGXH
WRDQWKURSRJHQLFSUHVVXUH&XUUHQWO\A. ursinum is not considered endangered and is allowed
IRU VXVWDLQDEOH XVH 7KH PDLQ KDELWDWV ZKHUH WKH VSHFLHV ZDV UHFRUGHG DUH Asperulo
FagetumEHHFKIRUHVWV%$0RHVLDQVLOYHU¿UIRUHVWV:0RHVLDQEHHFKIRUHVWV
Galio-CarpinetumRDNKRUQEHDPIRUHVWVAcidophilous Picea forests of the montane to
alpine levels (Vaccinio-Piceetea7KHKLJKHVWÀRULVWLFGLYHUVLW\ZDVUHFRUGHGLQWKHKDELWDW

Key words: medicinal plant, habitats, wild garlic
Bulgaria, Project DN 01/3

POPULATION STATUS AND HABITAT

CHARACTERISTIC OF TWO SIDERITIS SPECIES IN
BULGARIA AND GREECE
Ina Aneva1, Elina Yankova1, Peter Zhelev2, Rozalia Nikolova1

2
%DONDQ3HQLQVXODLVDUHJLRQRIKLJKGLYHUVLW\RISideritisVHFWLRQ(PSHGRFOLD7KHDLPRI
SUHVHQWVWXG\LVWRFKDUDFWHUL]HWKHSRSXODWLRQVWDWXVDQGKDELWDWVRIWZRHQGHPLFVSHFLHV±
Sideritis scardica*ULVHEIURP%XOJDULDDQG*UHHFHDQGSideritis euboea+HOGUIURP*UHHFH
7KH¿HOGREVHUYDWLRQVZHUHSHUIRUPHGLQ6ODY\DQND0WV5KRGRSHV0WV3LULQ0WV%XOJDULD
DQG2UYLORV0WV%R]GDJ0WV2O\PSRV0WVDQG'\U¿V0WV*UHHFH7KH*UHHNORFDOLWLHV
ZHUH LQ EHWWHU FRQGLWLRQ EDVHG RQ D V\VWHP RI LQGLFDWRUV OLNH SRSXODWLRQ VL]H GHQVLW\
YLDELOLW\ DQG QDWXUDOQHVV RI SODQW FRPPXQLWLHV S. scardica is collected for herbal tea both
in Bulgaria and Greece, but the studies have shown that Bulgarian localities are subjected to
FRQVLGHUDEO\ VWURQJHU DQWKURSRJHQLF SUHVVXUH$OVR %XOJDULDQ SRSXODWLRQV DUH LQ WKH PRVW
QRUWKHUQSHULSKHUDOSDUWRIWKHQDWXUDOGLVWULEXWLRQRIWKHVSHFLHV1DWXUDOSRSXODWLRQVRIWKH
narrow endemic S. euboea are in good condition which is illustrated by number of different
FULWHULD SRSXODWLRQ VL]H FRPSRVLWLRQ RI LWV QDWXUDO SODQW FRPPXQLWLHV ZHDN DQWKURSRJHQLF
SUHVVXUH 7KLV LV LOOXVWUDWHG E\ WKH SUHVHQFH RI QXPHURXV XQKDUYHVW LQÀRUHVFHQFHV DOUHDG\
ZLWK PDWXUH VHHGV LQ WKH HQG RI WKH YHJHWDWLRQ VHDVRQ HDUO\ 6HSWHPEHU 6HHG DQG SROOHQ
YLDELOLWLHVDVPHDVXUHGE\WHWUD]ROLXPDQGDFHWRFDUPLQHWHVWVUHVSHFWLYHO\ZHUHVLPLODULQ
WKH %XOJDULDQ DQG *UHHN SRSXODWLRQV DQG WKH GLIIHUHQFHV ZHUH QRW VWDWLVWLFDOO\ VLJQL¿FDQW
7KHÀRULVWLFLQYHQWRU\RIWKHQDWXUDOFRPPXQLWLHVRIWKHVWXGLHGVSHFLHVDOORZHGWRLGHQWLI\
WKHQDWXUDOKDELWDWVRIZKLFKWKH\DUHSDUW7KH\LQFOXGHGERWKIRUHVWDQGJUDVVODQGKDELWDWV
Forest habitats were dominated by Pinus nigra$UQ P. peuce Griseb, P. heldreichii Christ
and Abies cephalonica/RXG7KHUHVXOWVRIWKHVWXG\DUHGLVFXVVHGLQWKHOLJKWRIWKHVSHFLHV
FRQVHUYDWLRQDQGVXVWDLQDEOHXVH
Key words: Mountain tea, Sideritis, population status
Acknowledgement: The work was supported by the Program for career development of young
VFLHQWLVWV%$6*UDQWʋ')13

TRADITIONAL HERBAL TEAS CONSUMED IN MERSIN

(TURKEY)
7XUDQ$NGDJ6OH\PDQ'RJX+DYYD1XU'HPLU
'HSDUWPHQWRI$QLPDODQG3ODQWV3URGXFWLRQ0HUDP9RFDWLRQDO6FKRRO1HFPHWWLQ(UEDNDQ
8QLYHUVLW\.RQ\D7XUNH\
7UDGLWLRQDO KHUEDO WHDV KDYH EHHQ NQRZQ DQG ORYHG IRU \HDUV 7KH FXOWXUH UHODWHG WR
KHUEDOWHDVKDVEHHQFUHDWHGZLWKP\WKVLGLRPVDUWVDQGLQGXVWU\7KHSUHSDUDWLRQRIWHDKDV
EHHQGHWHUPLQHGE\UHJLRQRUFRXQWU\FXOWXUHDQGNQRZOHGJHDQGE\SRWHQWLDORIULFKÀRUDLQ
7XUNH\(LWKHUZLOGRUFXOWLYDWHGKHUEDOVDUHHYDOXDWHGDVWUDGLWLRQDOWHD+HUEDOWHDVDUHPDGH
XSRIVLQJOHSODQWVSHFLHVRUSODQWPL[WXUHV7KH\DUHFRQVXPHGIRUWKHLUSOHDVDQWDURPDWLF
ÀDYRUVDQGDOVRDVDLOPHQWVHJDJDLQVWFROGIDWLJXHDQGLQVRPQLD8VXDOO\KHUEDOWHDVDUH
REWDLQHG IURP URRW VWHP OHDI DHULDO SDUW DQG ÀRZHU DQG SUHSDUHG IRU GULQNLQJ LQ ERLOLQJ
ZDWHUDIWHUEHLQJGULHG9DULRXVVFLHQWL¿FVWXGLHVKDYHVKRZQWKDWSKHQROLFVXEVWDQFHVLQWHD
FRPSRVLWLRQKDYHDQWLR[LGDQWDQWLPLFURELDODQGDQWLFDUFLQRJHQLFSURSHUWLHV/RFDOSHRSOHRI
WKHFLW\RI0HUVLQKDYHEHHQFRQVXPLQJWUDGLWLRQDOKHUEDOWHDV3UHVHQWVWXG\LVDUHYLHZRI
WKHHWKQRERWDQLFDOVWXGLHVWKDWZHUHGRQHE\(YHUHVWHWDO(úHQ0HWLQ
6DGD\6D÷ÕUR÷OXHWDO6DUJÕQDQG(YHUHVWHWDORQWUDGLWLRQDO
KHUEDOWHDVLQ0HUVLQDQGLWVVXUURXQGLQJV$FFRUGLQJWRWKHUHVXOWVWD[DZHUHLGHQWL¿HGDV
WUDGLWLRQDOKHUEDOWHDVLQWKHVWXG\DUHDEHORQJLQJWRGLIIHUHQWIDPLOLHV7KHHQFRXQWHUHG
SODQW IDPLOLHV WUDGLWLRQDOO\ FRQVXPHG DV WHD ZHUH /DPLDFHDH WD[D$VWHUDFHDH WD[D
/DELDWDHWD[RQ/DXUDFHDHWD[RQ2OHDFHDHWD[RQ2UFKLGDFHDHWD[RQ5RVDFHDH
WD[RQ 6FURSKXODULDFHDH WD[RQ7LOLDFHDH WD[RQ DQG =LQJLEHUDFHD WD[RQ7KH
aerial parts, leaves, roots and fruits were determined as the most favored parts for traditional
KHUEDO WHDV 7UDGLWLRQDO KHUEDO WHDV DUH FRQVXPHG LQ D YDULHW\ RI ZD\V (WKQRERWDQLFDOO\
0HUVLQLVULFKLQÀRUDGXHWRLWVJHRJUDSKLFDOSRVLWLRQ$VDUHVXOWWUDGLWLRQDOKHUEDOWHDVDUH
VWLOOFRQVXPHGLQWKHFLW\RI0HUVLQ7XUNH\
Key words: traditional, herbal tea, Mersin

ETHNOBOTANICAL SURVEY OF MEDICINAL PLANTS

FROM ASTERACEAE IN BULGARIA
Svetlana Bancheva
Institute of Biodiversity and Ecosystem research, Bulgarian Academy of Sciences
The present study contains revision of the results on traditional use of wild medicinal plants
IURP$VWHUDFHDH LQ %XOJDULD 7KLV LV WKH ODUJHVW IDPLO\ RI YDVFXODU SODQWV LQ WKH %XOJDULDQ
ÀRUD ZLWK PRUH WKDQ VSHFLHV F RI WKH WRWDO ÀRUD 7KHUH KDYH EHHQ GHWHFWHG
$VWHUDFHDH VSHFLHV ZKLFK DUH EHLQJ XVHG ZLWK HWKQR WKHUDSHXWLF SXUSRVH 'LIIHUHQW KHUEDO
SDUWVDUHEDVLVIRUSUHSDUDWLRQRILQIXVLRQVGHFRFWWLQFWXUHV7KHPRVWVLJQL¿FDQWSODQWVRQ
WKH¿HOGRIHWKQRSKDUPDFRORJ\DUHDVIROORZVAchillea millefolium/Arctium tomentosum
0LOO Artemisia absinthium / Artemisia santonicum / Carduus acanthoides / Carlina
acanthifolia$OOCarlina vulgaris/Carthamus lanatus/Centaurea cyanus/Cichorium
intybus / Cnicus Benedictus / Helichrisum arenarium / 0RHQFK,, Inula helenium /
Matricaria chamomilla / Solidago virga-aurea /LQQ Sylibum marianum / *DHUWQ
Tanacetum vulgare / 7DUD[DFXP RI¿FLQDOH Weber ex Wigg, Tussilago farfara / HWF
Majority of herbs is being used for treatment of illnesses of respiratory, gastrointestinal,
IRUWUHDWPHQWRIVNLQFRQGLWLRQVDVZHOODVIRUQHUYRXVV\VWHPDQGHWF6RPHVSHFLHVSOD\V
important role as antioxidants in protecting cell membranes against pathological conditions
such as carcinogenesis, atherosclerosis, liver disease, prevention and treatment of cancer,
DJLQJPXWDJHQHVLV7KHHFRORJ\DQGSRSXODWLRQVVWDWXVRIVRPHVSHFLHVKDYHEHHQVWXGLHGDQG
UHFRPPHQGDWLRQVKDYHEHHQPDGHIRUWKHVXVWDLQDEOHXVHRIWKHLUUHVRXUFHV
Key words: Asteraceae, Bulgaria, medicinal plants

HOW DO MORPHOTYPES AND CHEMOTYPES RELATE

TO GENOTYPES? INTRASPECIES DIVERSITY OF
OCIMUM BASILICUM L.
.ODXGLMD&DURYLü6WDQNR1,2, Zlatko Liber2,3, Filip Varga1,2, Martina
Grdiša1,20LKDLOR5LVWLü4†=ODWNRâDWRYLü1,2

2
+5=DJUHE&URDWLD
3
University of Zagreb Faculty of Science, Division of Biology, Department of Botany,
0DUXOLüHYWUJD+5=DJUHE&URDWLD

,QVWLWXWHIRU0HGLFLQDO3ODQW5HVHDUFK³-RVLI3DQþLü´7DGHXãD.RãüXãND%HOJUDGH
Serbia
Basil (Ocimum basilicum / LV D ZLGHO\ NQRZQ PHPEHU RI /DPLDFHDH IDPLO\ ZLWK D ORQJ
WUDGLWLRQRIFXOWLYDWLRQ&XOWLYDWLRQIRUGLIIHUHQWPDUNHWUHTXLUHPHQWVOHGWRJUHDWLQWUDVSHFL¿F
YDULDWLRQDWERWKPRUSKRORJLFDODQGELRFKHPLFDOOHYHO7KHDLPRIWKLVUHVHDUFKZDVWRDVVHVV
diversity among basil accessions maintained at the Collection of Medicinal and Aromatic Plants
DVDSDUWRIWKH1DWLRQDO%DQNRI3ODQW*HQHV(LJKW\EDVLODFFHVVLRQVEHORQJLQJWR¿YHEDVLO
PRUSKRW\SHV7UXHEDVLO6PDOOOHDI/HWWXFHOHDI3XUSOHEDVLO$DQG3XUSOHEDVLO%DQG¿YH
FKHPRW\SHV+LJKOLQDORRO/LQDORROWUDQVĮEHUJDPRWHQH/LQDORROPHWK\OFKDYLFRO/LQDORRO
WUDQVPHWK\O FLQQDPDWH DQG +LJK PHWK\O FKDYLFRO ZHUH DQDO\VHG XVLQJ $)/3 PDUNHUV
6L[WHHQ$)/3SULPHUFRPELQDWLRQV\LHOGHGDWRWDORISRO\PRUSKLFPDUNHUV$QDO\VLVRI
PROHFXODUYDULDQFH$029$UHYHDOHGWKDWPRVWRIWKHJHQHWLFGLYHUVLW\ZDVDWWULEXWDEOHWR
GLIIHUHQFHVDPRQJDFFHVVLRQVZLWKLQPRUSKRW\SHVDQGZLWKLQFKHPRW\SHV%D\HVLDQ$QDO\VLV
of Population Structure (BAPS) resulted in assignment of the investigated accessions to four
FOXVWHUV7KH DFFHVVLRQV EHORQJLQJ WR µJUHHQ¶ PRUSKRW\SHV 7UXH EDVLO 6PDOOOHDI /HWWXFH
OHDIZHUHDVVLJQHGWR&OXVWHUV$RU%DQG3XUSOHEDVLO$DFFHVVLRQVWR&OXVWHUV$RU&3XUSOH
basil B morphotype was genetically the most admixed, having accessions belonging to all four
JHQHWLFFOXVWHUV
Key words:DFFHVVLRQ$)/3PDUNHUVEDVLOFKHPRW\SHVJHQHWLFGLYHUVLW\PRUSKRW\SHV
Acknowledgement: This work is part of the research programme on conservation of medicinal

and aromatic plants carried out by the Working Group on Medicinal and Aromatic Plants
(KWWSFSJUGKFSKVKUJEPDS), ZKLFK LV ¿QDQFHG E\ WKH 1DWLRQDO 3URJUDPPH IRU WKH
Conservation and Sustainable Use of Plant Genetic Resources for Food and Agriculture of
the R. of Croatia.

TRADITIONAL PREPARATION OF SYRUPS,

MARMALADES, LIQUEURS AND HERBAL GRAPPAS IN
THE KNIN AREA, CROATIA
,YDQDâROLü1, Filip Varga1,40DULMD-XJ'XMDNRYLü2, Lukasz Luczaj3,
Martina Grdiša1,4

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in Knin area in south-eastern Croatia during wider ethnobotanical survey on plant use in the
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BIOLOGICAL ACTIVITY AND TOXICITY (PART I)

Book of abstracts – 10th CMAPSEEC Plenary lecture
CHEMISTRY AND BIOACTIVITY OF SALVIA

OFFICINALIS L. AND RELATED SPECIES – A MULTI-
LAYERED PICTURE
Johannes Novak
University of Veterinary Medicine Vienna, Institute for Animal Nutrition and Functional
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Garden sage (6DOYLDRI¿FLQDOLV/*UHHNVDJHS. fruticosa0LOODQGDSSOHVDJHS. pomifera

/DUHWKUHHFORVHO\UHODWHGSalviaVSHFLHVQDWLYHWRWKH(DVWHUQ0HGLWHUUDQHDQEDVLQ7KHWKUHH
species are widely used as aromatic planta as well as in medicine for several reasons based
on different groups compounds, demonstrating a complex picture of chemical composition
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fruticosaLVULFKLQFLQHROHDQGS. fruticosa is rich in thujones, 6RI¿FLQDOLVis intermediate
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IRUWKXMRQHVLQWKH(XURSHDQ8QLRQIRUIRRGDQGSKDUPDFHXWLFDOSURGXFWVDUHGLIIHUHQW7KH
high antioxidant activity of sage is a further complex area, based on two different compound
groups, the (lipophilic) diterpenes carnosol and carnosic acid as well as on high amounts of
K\GURSKLOLFURVPDULQLFDFLG$IXUWKHULQWHUHVWLQJFRPSRXQGLVWKHSHQWDF\FOLFWULWHUSHQRLG
XUVROLFDFLGZKLFKLVXVHGDVDQHPXOVL¿HUDQGLVDFWLYHDJDLQVWEUDLQGLVRUGHUV7KHPDQ\
different activities in sage based on many different compounds or compound groups do not
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GLIIHUHQWLDWLRQLQWREUHHGLQJOLQHVZLWKGLIIHUHQWPD[LPL]HGDFWLYLWLHV
Key words: 6DOYLDRI¿FLQDOLV, monoterpenes, thujones, antioxidant activity, carnosol,

carnosic acid, rosmarinic acid, ursolic acid

ANALYSIS OF AROMATIC AND MEDICINAL PLANTS

APPLYING VARIOUS VIBRATIONAL SPECTROSCOPY
TECHNIQUES - POSSIBILITIES AND LIMITATIONS
Hartwig Schulz
Julius Kühn-Institute (JKI), Institute for Ecological Chemistry, Plant Analysis and Stored
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WHFKQLTXHV)RUWKLVWHVWLQJRISODQWPDWHULDOVXFKDVSK\WRSKDUPDFHXWLFDOSURGXFWVLVXVXDOO\
performed in accordance with validated standard methods described in the Food Chemical
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to this approach, there is also a need to apply various rapid high-throughput methods aiming
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NIR and Raman spectroscopy) in combination with various chemometric algorithms are
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Raman spectroscopy has especially been found to be a reliable and non-destructive method for
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spectrometer systems are available which need only small sample amounts of a few microliters
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on plant tissues as well as on fractions isolated from the plant material by hydro-distillation
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allow the discrimination of different species, and even to classify chemotypes among the same
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Key words:TXDOLW\FRQWUROFKHPRW\SHV,55DPDQ

IN VITRO ANTIFUNGAL POTENCY OF EXTRACT

FROM ANISE FRUITS (PIMPINELLA ANISUM L.)
AND FIVE ESSENTIAL OILS AGAINST SOME PLANT
PATHOGENIC FUNGI
Natalija Atanasova-Pancevska, Dzoko Kungulovski
Department of Microbiology and Microbial Biotechnology, Institute of Biology, Faculty

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Macedonia
The negative environmental impact of pesticides used for disease control in plants is intensively
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extract from anise fruits (Pimpinella anisum/DQG¿YHHVVHQWLDORLOV_-pinene, `-pinene,
estragole, fenchone and trans-anethole) were investigated in vitroDJDLQVW¿YHLPSRUWDQWSODQW
pathogenic fungi (Alternaria alternata, Fusarium oxysporum, Rhizoctonia solani, Botrytis
cinerea and Plasmopara viticolaDWGLIIHUHQWFRQFHQWUDWLRQVPLPL'062
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Department of Microbiology and Microbial Biotechnology, Institute of Biology, Faculty of
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vitro DQWLPLFURELDO VFUHHQLQJ 7KH PHWKRGRORJ\ XVHG LV EDVHG RQ PHDVXULQJ WKH 0LQLPXP
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compared with controls, the highest antifungal activity of extract from anise fruits (Pimpinella
anisum/ZDVUHFRUGHGIRUFusarium oxysporum, Botrytis cinerea and Plasmopara viticola at
DFRQFHQWUDWLRQRI0)& PLPL'062DQG0,&FRQFHQWUDWLRQZDVPLPL
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microorganism was Botrytis cinerea 7KH KLJKHVW FRQFHQWUDWLRQ RI 0,& DQG 0)& VKRZHG
fenchone (25 mL mL'062DQGPLPL'062UHVSHFWLYHO\ZLWKPlasmopara
viticola and Alternaria alternata7KHUHVXOWVLQGLFDWHGWKDWDOOWHVWHGVXEVWDQFHVKDGDQWLIXQJDO
DFWLYLW\DJDLQVWDOOWHVWHGIXQJLLQGLIIHUHQWFRQFHQWUDWLRQV7KHVHVXEVWDQFHVRUVRPHPL[WXUH
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study paves the way for the development of bioactive natural products with phytosanitary
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Key words: antifungal, phytopathogen, essential oil, inhibition

THE CLOVE ESSENTIAL OIL AND ITS MAJOR

COMPONENT EUGENOL SUCCESSFULLY RESTORED
ERECTILE DYSUNCTION OF STREPTOZOTOCIN-
INDUCED TYPE I DIABETES IN RATS
Alev Önder1, Serap Gur1, Didem Yilmaz-Oral1,2

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2
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duration of diabetes and PDE5i are not as effective in the treatment of diabetes-associated
('&XUUHQWO\VHYHUDOPHGLFLQDOSODQWVDQGVHFRQGDU\PHWDEROLWHVDUHQDWXUDOUHPHGLHVIRU
('$WURSLFDOSODQWSyzygium aromaticum/0HUULOO 3HUU\RUFORYHIURPWKH0\UWDFHDH
IDPLO\KDVDSKURGLVLDFDFWLYLW\,Q(XURSHDQG$VLDLWKDVEHHQXVHGIRUFXOLQDU\DQGPHGLFLQDO
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VWUHSWR]RWRFLQLQGXFHGGLDEHWLFUDWV$GXOWPDOHWZHQW\6SUDJXH'DZOH\UDWVZHUHGLYLGHGLQWR
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UDWFRUSXVFDYHUQRVXP&&LQRUJDQEDWKVWXGLHV'LDEHWLFUDWVKDGVLJQL¿FDQWO\GHFUHDVHGin
vivoHUHFWLOHUHVSRQVHVZKLFKZHUHLPSURYHGDIWHULQWUDFDYHUQRVDODGPLQLVWUDWLRQRI&2DQG
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Key words: clove, ED, Syzygium aromaticum, diabetes

AMELIORATIVE EFFECTS OF MATRICARIA

CHAMOMILLA L. HYDROALCOHOLIC EXTRACT ON
SCOPOLAMINE-INDUCED MEMORY IMPAIRMENT IN
RATS: A BEHAVIORAL AND MOLECULAR STUDY
Radu Ionita1, Paula Alexandra Postu1, Marius Mihasan1, Dragos Lucian
Gorgan1, Cornelia Mircea2, Monica Hancianu2, Lucian Hritcu1, Oana
Cioanca2

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investigate the effects of the hydroalcoholic extract of M. chamomilla on memory processes
in a scopolamine-induced a rat model of amnesia and to reveal its underlying mechanism of
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along with the total content of reduced glutathione and protein carbonyl and malondialdehyde
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acid, apigenin-7-glucoside, rutin, cynaroside, luteolin, apigenin and derivatives of apigenin-7-
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and the oxidant-antioxidant balance in the rat hippocampus was recovered by the treatment
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suggest that the extract could be a potent neuropharmacological agent against amnesia via
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ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS

OF PINUS NIGRA J.F. Arnold AND PINUS SYLVESTRIS L.
COMPARE WITH ALPHA- AND BETA- PINENE
Natalija Atanasova-Pancevska, Dzoko Kungulovski
Department of Microbiology and Microbial Biotechnology, Institute of Biology, Faculty

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Macedonia
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nigra -) $UQROG DQG Pinus sylvestis / IURP 0DFHGRQLD ZHUH DQDO\VHG 7KHVH UHVXOWV
were compared with in vitro antibacterial activites of _< and `< pinene, as one of the main
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microorganism we used a panel of different bacteria, including four Gram negative bacteria
(E. coli ATCC 8739, Ps. aeruginosa$7&&S. typhimurium, S. enteridis) and nine Gram
positive bacteria (B. subtilis$7&& B. pumillus 1&7& Bacillus VS S. citrus,
S. albus, S. aureus, M. luteus, L. monocytogenes and S. lutea7ZR WHVWHG H[WUDFWV VKRZHG
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all Gram positive bacteria showed as more sensitive against all tested substances compare to
*UDPQHJDWLYHEDFWHULD&UXGHH[WUDFWVIURPPinus nigra showed as more potent against Gram
negative bacteria compare to extract from Pinus sylvestris and Ps. aeruginosa$7&&
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*UDPSRVLWLYHEDFWHULDWKHUHDUHQRDQ\VLJQL¿FDQWGLIIHUHQFHVZLWKLQDFWLYLW\RIH[WUDFWVIURP
P. nigra and P. sylvestris )URP *UDP SRVLWLYH EDFWHULD S. citrus showed biggest sensitivity
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`-pinene, in this screening _-pinene showed better results from `-pinene for both groups of
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VXEVWDQFHV,WZDVFRQFOXGHGWKDWWKHWHVWHGH[WUDFWVVKRZHGSURPLVLQJDQWLPLFURELDODFWLYLW\
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Key words: antibacterial, Pinus, extract, broth-microdilution assays

BOTANICALS IN FOOD SUPPLEMENTS AND NOVEL

FOOD
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Based on the development of self-healing awareness, food supplements are experiencing growth,
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attention to whether some of the ingredients are in novel foods or are the ingredients of a new
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Key words: botanicals, food supplements, novel food, labelling, legislation frame, European
Union, Republic of Croatia

PHYTOCHEMICAL EVALUATION OF IN VITRO

CULTURES FROM ASTRAGALUS GLYCYPHYLLOS L.
Aleksandar Shkondrov, Pavlinka Popova, Ilina Krasteva, Petranka
Zdraveva, Iliana Ionkova
'HSDUWPHQWRI3KDUPDFRJQRV\)DFXOW\RI3KDUPDF\0HGLFDO8QLYHUVLW\RI6R¿D
'XQDY6W6R¿D%XOJDULD
Astragalus glycyphyllos/)DEDFHDHLVDSHUHQQLDOKHUEDFHRXVSODQWZLGHO\GLVWULEXWHGLQ

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DFWLYHVXEVWDQFHVSURYHGLQWKHVSHFLHV±SRO\VDFFKDULGHVVDSRQLQVDQGÀDYRQRLGV6HYHUDO
pharmacological studies revealed the hepatoprotective and antioxidant potential of extracts
IURPWKHSODQW7KHDLPRIWKHSUHVHQWVWXG\ZDVWRHVWDEOLVKin vitro cultures of A. glycyphyllos
DQG WR LQYHVWLJDWH WKH TXDQWLW\ RI ÀDYRQRLGV DQG VDSRQLQV LQ H[WUDFWV IURP HDFK FXOWXUH$
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parts of large intact A. glycyphyllosSODQWVZHUHXVHGWRIRUPFDOOXV6XVSHQVLRQFXOWXUHVZHUH
HVWDEOLVKHGE\WUDQVIHUULQJVPDOOSLHFHVRIFDOOXVWROLTXLGPHGLXPZKLFKZDVVXEVHTXHQWO\
SODFHG RQ D J\UDWRU\ VKDNHU 5XWLQ ZDV SURYHG LQ KLJKHVW DPRXQW LQ VKRRW FXOWXUHV RQ 06
FXOWXUHPHGLXPQJPJGZ&DPHOOLDVLGH$ZDVGHWHUPLQHGLQKLJKHVWTXDQWLW\LQVKRRWV
QJPJGZ6DSRQLQ6ZDVTXDQWL¿HGDVQJPJGZIRUWKHVDPHFXOWXUH
$OOFXOWXUHVGHYHORSHGLQWKHGDUNSURYHGORZHVWDPRXQWVRIDQDO\WHVGHVSLWHRIKRUPRQHV
&DOOXVHV JURZQ LQ OLJKW GLVSOD\HG KLJKHU TXDQWLW\ LI WKH WKUHH PRQLWRUHG FRPSRXQGV WKDQ
VXVSHQVLRQFXOWXUHV7KHUHVXOWVRIWKHVWXG\LQGLFDWHWKDWin vitro cultures of A. glycyphyllos
FRXOGVHUYHDVDQDOWHUQDWLYHZD\IRUSURGXFWLRQRIÀDYRQRLGVDQGVDSRQLQV
Key words: Astragalus glycyphyllos, in vitroFXOWXUHVVDSRQLQVÀDYRQRLGV8+3/&

+5(6,06
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BIOGENETIC RELATIONSHIP BETWEEN SOME INULA

SPECIES GROWING IN BULGARIA BASED ON THEIR
PHYTOCHEMICAL PROFILE
$QWRDQHWD7UHQGD¿ORYD1, Victoria Ivanova1, Milka Todorova1, Ina
Aneva2

2
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences,
6R¿D%XOJDULD
Inula$VWHUDFHDHLVDODUJHJHQXVZLWKPRUHWKDQVSHFLHVGLVWULEXWHGLQ$IULFD$VLDDQG

(XURSHInula helenium/Inula racemosa+RRNIDQGInula britannica/DUHIUHTXHQWO\XVHG
LQHWKQRPHGLFLQHInulaVSHFLHVDUHFRQWLQXRXVO\WKHWDUJHWRIVFLHQWL¿FLQYHVWLJDWLRQVWR¿QG
their active principles and new pharmacological applications, or from chemotaxonomical point
RI YLHZ 5HFHQWO\ ZH KDYH VWDUWHG SK\WRFKHPLFDO LQYHVWLJDWLRQ RI Inula species growing in
%XOJDULD([WUDFWVIURPI. helenium, I. aschersoniana-DQNDI. britannica/I. oculus-christi/
I. germanica/I. conyza *ULHVV0HLNOHI. ensifolia/DQGI. bifrons//ZHUHVWXGLHG,W
ZDVIRXQGWKDWWKHPHWKDQROH[WUDFWVRIDOORIWKHPFRQWDLQHGFKORURJHQLFDQG
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LQWKLVFDVH)XUWKHUWKHODFNRIVHVTXLWHUSHQHODFWRQHVLQI. conyza, I. ensifolia, and I. bifrons
GLVWLQJXLVKHGWKHVHVSHFLHVIURPWKHRWKHUIRXU:LWKH[FHSWLRQRII. germanicaVHVTXLWHUSHQH
lactones were studied in I. helenium (alantolactone, isoalantolactone), I. aschersoniana
(parthenolide, diepoxycostunolide, inusoniolide), I. britannica (ivalin, britannin, gaillardin,
GLK\GURLQXFKLQHQROLGH % SXOFKHOOLQ & I. oculus-christi (gaillardin, pulchellin E,
SXOFKHOOLQ & ȕȕHSR[\JDLOODUGLQ ĮĮHSR[\HSLJDLOODUGLQ ĮDFHWR[\Įȕ
GLK\GUR[\ȕJXDLDGLHQĮROLGH ĮĮHSR[\SXOFKHOOLQ ( ,W KDV EHHQ
IRXQG WKDW VSHFLHV SURGXFHG GLIIHUHQW W\SH RI ODFWRQHV ROLGHV LQ I. aschersoniana and
ROLGHV LQ WKH RWKHU VSHFLHV$QRWKHU VLJQL¿FDQW IHDWXUH IRU I. aschersoniana was the
SURGXFWLRQRIJHUPDFUDQROLGHVDQGDVHFRJXDLDQROLGH(XGHVPDQROLGHVLYDOLQDQGLWV&
oxygenated derivatives- pulchellin B and C) and guaianolides (gaillardin and biogenetically
related to it) isolated from I. britannica and I. oculus-christi VKRZHG WKHLU VLPLODULW\ 7KH
eudesmanolides found in I. heleniumLQGLFDWHGWKDWLWLVFORVHUWRWKHODWWHUWZRVSHFLHV7KH
obtained results could help in determining the place of these species within the general scheme
RIWKHJHQXV
Key words: Inula$VWHUDFHDHVHVTXLWHUSHQHODFWRQHVELRJHQHWLFUHODWLRQVKLS
Bulgaria, Project DN 09/11

ANTIMICROBIAL AND ANTIFUNGAL ACTIVITIES OF

METHANOL EXTRACT OF ARGEMONE MEXICANA L.
AND ITS ANTI-HEPATITIS POTENTIAL
Yusuf Haruna, Ukamaka
Department of Medicinal Chemistry, Faculty of Science, Kebbi State University of Science

and Technology, Aliero
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LVRQHRIWKHPRVWIDVFLQDWLQJVFLHQWL¿FDGYHQWXUHVIRUKDOIRIWKHFHQWXU\,QGLYLGXDOL]DWLRQ
RIVHYHUDOW\SHVRIKHSDWLWLVRQO\HPHUJHGDIWHUZRUOGZDUWZR7KHLULGHQWL¿FDWLRQKDVEHHQ
DVVRFLDWHGZLWKPLOHVWRQHVZKLFKUHYROXWLRQL]HGPHGLFLQHDQGSXEOLFKHDOWK7KHGLVFRYHU\RI
+%9EURXJKWWKH¿UVWHYHUYDFFLQHQRWSUHSDUHGE\WLVVXHFXOWXUHEXWLQLWLDOO\GLUHFWO\IURP
SODVPDDQGVRRQDIWHUWKDWWKH¿UVWYDFFLQHZDVSURGXFHGE\JHQHWLFHQJLQHHULQJ,QFUHDVLQJ
HYLGHQFHVVXJJHVWWKDW9LUDO+HSDWLWLVLVDFULWLFDOGLVHDVHWKDWNLOOVDERXWWRSHRSOH
SUHPDWXUHO\ GXH WR LWV LQIHFWLRQ7UHDWPHQW ZLWK DYDLODEOH LQWHUIHURQV RQO\ VXSSUHVVHV YLUDO
UHSURGXFWLRQ LQ DERXW WR RI SDWLHQWV ZLWK FKURQLF KHSDWLWLV 0RVW SHRSOH GR QRW
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'HWHUPLQDWLRQ RI LQKLELWLRQ ]RQH RQ DJDU PHGLD XVHG LQ WKLV VWXG\ VKRZHG WKDW Argemone
mexicana/H[WUDFWH[KLELWHGDQWLPLFURELDOHIIHFWVDJDLQVWE. coli, B. subtilis and S. aureus,
DWDQGPJPLUHVSHFWLYHO\7KHDQWLIXQJDODFWLYLW\RIWKHH[WUDFWVRIWKHVDPH
concentrations against A. niger, A. fumigatus and M. VSHFLHV ZDV GRVH GHSHQGHQW 7KH
SK\WRFKHPLFDOVFUHHQLQJRIWKLVSODQWUHYHDOHGWKHSUHVHQFHRIWDQQLQVVDSRQLQVÀDYRQRLGV
WHUSHQRLGV DONDORLGV JO\FRVLGHV HWF ZKLFK DUH UHSRUWHG WR EH UHVSRQVLEOH IRU QXPHURXV
PHGLFLQDO XVHV RI SODQWV A. mexicana is a species whose pharmacological properties such
DVDQWLKHSDWRWR[LFDFWLYLW\DQWLPDODULDODQWLEDFWHULDOKHSDWRSURWHFWLYHDQWL+,9DQGDQWL
KHSDWLWLV KDYH EHHQ GHVFULEHG E\ WKH WUDGLWLRQDO PHGLFDO SUDFWLWLRQHUV ,Q WKH WUHDWPHQW RI
MDXQGLFHLWKDVEHHQEHHQFRQ¿UPHGE\VHYHUDODXWKRUVKHQFHWKHUHLVDQHHGIRUIXUWKHUVWXGLHV
It is therefore important, for us as scientist responsible for the discovery of noble drugs, to
FKDUDFWHUL]HWKLVSODQWLQRUGHUWR¿QGVDIHUSRWHQWGUXJVIRUWKLVVFRXUJHKHSDWLWLV
Key words: Argemone mexicana, bacterial, extract, fungi, hepatitis

HOW DOES ABIOTIC STRESS INFLUENCE A HEALTH-

PROMOTING PHYTOCHEMICALS IN BRASSICA
CROPS?
Branka Salopek-Sondi1,GD/LQLü1,YD3DYORYLü1, Jiri Gruz2, Dunja
Šamec1

'HSDUWPHQWIRU0ROHFXODU%LRORJ\5XÿHU%RãNRYLü,QVWLWXWH&URDWLD
2
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$6&5&]HFK5HSXEOLF
&URSSODQWVIURPWKH%UDVVLFDFHDHIDPLO\LQFOXGLQJFDEEDJHVNDOHVEURFFROLHWFDUHDPRQJ
WKH ROGHVW NQRZQ FXOWLYDWHG SODQWV DQG DUH DOVR FRPPHUFLDOO\ LPSRUWDQW YHJHWDEOHV ZRUOG
ZLGH7KH\KDYHDQLPSRUWDQWSODFHLQWKHFXOWXUHDQGWUDGLWLRQDOFXLVLQHRIZRUOGZLGHDQGDUH
ZLGHO\XVHGLQWUDGLWLRQDOPHGLFLQH'XHWRDIIRUGDEOHSULFHDQGDYDLODELOLW\DWORFDOPDUNHWV
Brassicaceae stands out as an important source of food, providing nutrients and health-
SURPRWLQJ SK\WRFKHPLFDOV JOXFRVLQRODWHV SKHQROLF FRPSRXQGV HWF ZKLFK SDUWLFXODUO\
DWWUDFWHG PXFK UHFHQW VFLHQWL¿F DWWHQWLRQ ,Q WKH OLJKW RI FOLPDWH FKDQJHV Brassica crops
PD\EHKLJKO\LQÀXHQFHGE\XQSOHDVDQWHQYLURQPHQWDOFRQGLWLRQVRUGLYHUVHDELRWLFVWUHVVHV
+HUHLQZHLQYHVWLJDWHGWKHLPSDFWRIDELRWLFVWUHVVHVRQWKHSUR¿OHRIVSHFLDOL]HGPHWDEROLWHV
in Brassica species traditionally grown in Croatia (Brassica oleracea/YDUcapitataIalba
FYµ9DUDåGLQVNL¶DQGB. oleraceaYDUacephala), and compared with B. rapaVVSpekinensis,
VSHFLHV NQRZQ DV PRGHUDWH VHQVLWLYH WR DELRWLF VWUHVVHV 7KH UHVXOWV VKRZHG WKDW DELRWLF
VWUHVV FDXVHG TXDOLWDWLYH DQG TXDQWLWDWLYH FKDQJHV LQ VSHFLDOL]HG PHWDEROLWHV SDUWLFXODUO\
SRO\SKHQROLFVDQGDQWLR[LGDQWDFWLYLW\GHSHQGRQVSHFLHVDQGDSSOLHGVWUHVV7KHVHFKDQJHV
LQ VSHFLDOL]HG PHWDEROLWHV DUH GLVFXVVHG LQ UHODWLRQ WR VWUHVV WROHUDQFH RI GLIIHUHQW Brassica
VSHFLHVYDULHWLHV
Key words: BrassicaFURSVZKLWHFDEEDJHNDOHDELRWLFVWUHVVSK\WRFKHPLFDOV

MOLECULAR MODELING AND MOLECULAR

DOCKING FOR MEDICINAL PLANT VALORIZATION
Salim Bouchentouf
'RFWRU7DKDU0RXOD\8QLYHUVLW\RI6DLGD$OJHULD/DERUDWRU\RI1DWXUDO3URGXFWVDQG
Bioactives, Tlemcen
Interest to medicinal and aromatic plants for therapeutic purposes is a highly sought-after area
RIUHVHDUFKDQGDQDUHDWKDWKDVQRW\HWUHYHDOHGDOOLWVVHFUHWV$URXQGWKHZRUOGUHVHDUFKHUV
identify the therapeutic virtues of different plants from different regions while identifying the
chemical components, in particular the bioactive molecules whose study of their effects for
WKHWUHDWPHQWRIFHUWDLQSDWKRORJLHVUHPDLQVORQJDQGVRPHZKDWGLI¿FXOWJLYHQWKHFRPSOH[LW\
RI WKH SURFHGXUH ,Q WKLV FRQWH[W PROHFXODU PRGHOLQJ DQG 0ROHFXODU 'RFNLQJ KDV EHHQ
DGRSWHGDVDQLPSRUWDQWVWHSEHIRUHLQYHVWLJDWLRQSODQWYLUWXHVLQWKHWUHDWPHQWRIDQ\GLVHDVH
0ROHFXODU 'RFNLQJ LV D FRPSXWDWLRQDO PHWKRG WKDW VDYHV WLPH DQG PRQH\ LQ VWXG\LQJ WKH
HIIHFWRIELRDFWLYHPROHFXOHVEHIRUHWHVWLQJDQGGHVLJQLQJGUXJV0RVWRIWKHSKDUPDFHXWLFDO
ODERUDWRULHVXVH0ROHFXODU'RFNLQJWRLGHQWLI\WKHEHVWELRDFWLYHPROHFXOHVFDSDEOHWRLQKLELW
HQ]\PHVUHVSRQVLEOHRISDWKRORJLHV7KLVSUHVHQWDWLRQZLOOJLYHDQLQVLJKWWRWKLVPHWKRGDV
ZHOODVLWVDGYDQWDJHVIRUWKHYDORUL]DWLRQRIDURPDWLFDQGPHGLFLQDOSODQWVYLDWKHLUFKHPLFDO
FRPSRVLWLRQ
Key words:0ROHFXODU'RFNLQJELRDFWLYHVPROHFXOHVWKHUDSHXWLFHIIHFWVSODQWV
YDORUL]DWLRQ

EVALUATION OF THE POLYPHENOLIC AND

PHYTOSTEROLIC CONTENT FROM SCIRPOIDES
HOLOSCHOENUS (L.) Soják
&DUPHQ(ğHEUHQFX1,3, Elvira Gille2, Oana T. Ciuperca1, Radu
Necula2,4, Ruxandra M. Cretu2, Ionescu Elena1,3

7KH&RPPHUFLDO6RFLHW\IRU0HGLFLQDO3ODQW5HVHDUFKDQG3URFHVVLQJ´3/$17$925(/´
6$&X]D9RGD6WUHHW3LDWUD1HDPW5RPDQLD
2
1,5'%6´6WHMDUXO´%LRORJLFDO5HVHDUFK&HQWUH$OH[DQGUXFHO%XQ3LDWUD
Neamt, Romania
3
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)DFXOW\RI&KHPLVWU\?´$OH[DQGUX,RDQ&X]D?´8QLYHUVLW\,DVL5RPDQLD
Scirpoides holoschoenus/6RMiN&\SHUDFHDHLVDVSHFLHVNQRZQVLQFHDQWLTXLW\DQGXVHG

LQ WKH HWKQR PHGLFLQH RI ¿VKHUPHQ DQG VDLORUV LQ WKH 'DQXEH 'HOWD DQG WKH %ODFN 6HD DV
a decoction for maintaining and preserving the health of the digestive, biliary and hepatic
WUDFWEXWQRWXVHGLQPRGHUQWKHUDS\WKHFRPSRVLWLRQLQDQWLR[LGDQWDQWLLQÀDPPDWRU\DQG
KHSDWRSURWHFWLYHSULQFLSOHVLVOLWWOHNQRZQ7KHDLPRIWKHSUHVHQWVWXG\ZDVWRLGHQWLI\DQG
TXDQWLI\ VHYHUDO SRO\SKHQROLF FRPSRXQGV DQG SK\WRVWHUROV RI WKH Scipoides holoschoenus
SODQW DHULDO SDUWV DQG URRW 7KH LGHQWL¿FDWLRQ DQG TXDQWL¿FDWLRQ RI WKHVH FRPSRXQGV ZDV
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limited range of phenolic and sterolic substances: chlorogenic acid, ferulic acid, rosmarinic
DFLG DSLJHQLQ OXWHROLQ ȕVLWRVWHURO DQG VWLJPDVWHURO 4XDQWLWDWLYH GHWHUPLQDWLRQV UHYHDOHG
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URRWPJUHVSHFWLYHO\PJPLDQGSFRXPDULFDFLGPJUHVSHFWLYHO\PJ
PL E\ +3/& FKURPDWRJUDSK\ PHWKRG 7KH REWDLQHG UHVXOWV PD\ VHUYH WR SURPRWH
the use of the Scirpoides holoschoenus/VSHFLHVDVDQWLR[LGDQWDQGKHSDWRSURWHFWLYHDJHQWV
Key words: Scirpoides holoschoenus/DQWLR[LGDQWV)7,5VSHFWURIRWRPHWU\+37/&

FKURPDWRJUDSK\+3/&FKURPDWRJUDSK\

BIOACTIVE COMPOUNDS IN BRASSICA OLERACEA L.

VEGETABLES TRADITIONALY GROWN IN CROATIA
Dunja Šamec13HWUD,YDQLãHYLü1,YD3DYORYLü1,YDQD5DGRMþLü
5HGRYQLNRYLü2, Branka Salopek-Sondi1

5XÿHU%RãNRYLü,QVWLWXWH'HSDUWPHQWRI0ROHFXODU%LRORJ\%LMHQLþNDF=DJUHE
Croatia
2
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Zagreb, Croatia
&UXFLIHURXVYHJHWDEOHVDUHUHFRJQL]HGDVDIXQFWLRQDOIRRGEHFDXVHGLIIHUHQWHSLGHPLRORJLFDO
and meta-analysis suggested that consumption of cruciferous has preventive role against a
variety of disease due to the presence of the bioactive compounds such as glucosinolates,
SRO\SKHQROVFDURWHQRLGVHWF,QWKHSUHVHQWVWXG\ZHDLPHGWRLGHQWLI\ELRDFWLYHFRPSRXQGV
(glucosinolates, polyphenols, carotenoids, ascorbic acid) and to measure antioxidant activity
RI ZKLWH FDEEDJH FY 9DUDåGLQVNL &URDWLDQ QDPH YDUDåGLQVNR ]HOMH /DWLQ QDPH Brassica
oleracea / YDU capitata I alba DQG ÀDW OHDYHV NDOH &URDWLDQ QDPH UDãWLND /DWLQ QDPH
Brassica oleracea/YDUacephala), two B. oleracea vegetables commonly used in Croatian
FRXVLQ DQG WUDGLWLRQDO PHGLFLQH ,Q DGGLWLRQ ZH DOVR PHDVXUHG DFWLYLW\ RI HQGRJHQRXV
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899,6PHWKRGV,QWKHVWXG\ZHLQFOXGHYHJHWDEOHVDWGLIIHUHQWGHYHORSLQJVWDJHVDVZHOO
DV IURP GLIIHUHQW VHHG SURGXFHUV 5HVXOWV VKRZHG WKH SUHVHQFH RI WKH ELRDFWLYH FRPSRXQGV
DVVRFLDWHGZLWKKHDOWKEHQH¿WVDQGVLJQL¿FDQWDQWLR[LGDQWDFWLYLW\LQERWKB. oleraceaYDULHWLHV
%DVHG RQ FRPSDUDWLYH DQDO\VLV RI GLIIHUHQW FUXFLIHURXV YHJHWDEOHV ZKLWH FDEEDJH DQG NDOH
VKRZKLJKHUDPRXQWRISRO\SKHQROVDQGJOXFRVLQRODWHVWKDQ&KLQHVHFDEEDJHRUEURFFROL
Key words: Brassica, glucosinolates, polyphenols, carotenoids, Croatia

PROTECTIVE POTENTIAL OF LYOPHILIZED EXTRACT

OF CLINOPODIUM VULGARE L. (LAMIACEAE) IN
SPONTANEOUSLY HYPERTENSIVE RATS (SHRS)
Rumyana Simeonova1, Vessela Vitcheva1, Magdalena Kondeva-
Burdina1, Dimitrina Zheleva2, Vessela Balabanova2, Reneta Gevrenova2

Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy,
0HGLFDO8QLYHUVLW\RI6R¿D´'XQDY´VWU6R¿D
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Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical
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EHHQWKRXJKWWREHHIIHFWLYHIRUWKHSUHYHQWLRQDQGFRQWURORI+71,QWKLVVWXG\O\RSKLOL]HG
extract of Clinopodium vulgare / /DPLDFHDH /(&9 ZDV DQDO\]HG E\ XOWUDKLJK
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observed effects on tissue level were consistent with the histopathological observations of the
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Key words: Clinopodium vulgare /.,6+5R[LGDWLYHVWUHVVDQWLR[LGDQWHQ]\PHV
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0HGLFDO6FLHQFH3URMHFWʋ&RQWUDFW'

UHPLC-HRMS BASED FLAVONOID PROFILING OF THE

AERIAL PARTS FROM CHENOPODIUM FOLIOSUM Asch.
(AMARANTHACEAE)
Zlatina Kokanova-Nedialkova, Paraskev Nedialkov
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6R¿D%XOJDULD
The genus Chenopodium$PDUDQWKDFHDHKDVEHHQHVWLPDWHGWRFRPSULVHDERXWVSHFLHV

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Key words: Chenopodium foliosum$PDUDQWKDFHDHÀDYRQRLGV8+3/&+506

PHYTOCHEMICAL EVALUATION OF SOME FOOD

SUPPLEMENTS WITH MELISSA OFFICINALIS L. FROM
ROMANIA
Elvira Gille1, Ruxandra Mihaela Cretu1, Valentin Grigoras1, Radu
Necula1,2, Dana Bobit3, Georgiana Luminita Gavril1

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2
3
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The dried leaves are long used in traditional and modern medicine for headaches, mental stress,
nervous disturbances of sleep, cardiovascular, respiratory and gastrointestinal problems, and as
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and sedative) have been attributed mainly to its essential oil (citral A and B) and phenolic
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bioactive compounds responsible for their phytotherapeutic properties: polyphenols (gallic
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evaluation 0RI¿FLQDOLV essential oils by GC-MS indicated the presence of the main volatile
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Key words: lemon balm, food supplements, rosmarinic acid, volatile fractions

VOLATILE COMPOUNDS FROM FOENICULUM

VULGARE MILL. YELLOW LEAVES OBTAINED BY
SUPERCRITICAL FLUID EXTRACTION
Nina Djapic, Vjekoslav Sajfert
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Zrenjanin, Serbia
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ÀXLG H[WUDFWLRQ ZLWK &22 was used for the extraction of volatile compounds from yellow
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ZHUHSUHVHQWLQWKHDPRXQWRI'LWHUSHQHVSUHVHQWLQWKHH[WUDFWZHUHQHRSK\WDGLHQH
DQGR[\JHQDWHGGLWHUSHQHSK\WRO,QWKH\HOORZFoeniculum vulgare leaves
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can be used for the isolation of the most abundant compounds: 2-acetylcyclopentanone and
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Key words: Foeniculum vulgare, yellow leaves, SFE

POLYPHENOL DIVERSITY AND IN VITRO

ANTIOXIDANT PROPERTIES OF TUBERS OF
ORNAMENTAL DAHLIA VARIETIES
Adam Matkowski1, Sonia Pandel2, Ewa Mura2, Sebastian Granica3,
Anatoliy Doroshenko4, Nonna Ilyinska5, Tatiana Gontova5, Yana
Kriukova5

Department of Pharmaceutical Biology and Botany, Medical University, Wroclaw, Poland
2
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University of Wroclaw
3
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical
University, Poland

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Dahlias are popular ornamental plants, but they are also used as alternative food source with
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x hortensis $V PHPEHUV RI WKH $VWHURLGHDH VXEIDPLO\ GDKOLDV DUH D SRWHQWLDO VRXUFH RI
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DQGDFWXDOFRQWHQWRIWKHVHFRPSRXQGVLQGLIIHUHQWSDUWVRIWKHSODQWV,QWKLVVWXG\ZHDQDO\]HG
the diversity of phenolic compounds in extracts from leaves and tubers of nine differently
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tested for the antioxidant activity using in vitroVSHFWURSKRWRPHWULFDVVD\V'33+VFDYHQJLQJ
phosphomolybdenym reduction, polyunsaturated fatty acids peroxidation) and inhibition of
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the plant material, and unproblematic growing, dahlias which are also generally considered as
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Key words:'DKOLD$VWHUDFHDHFDIIR\OTXLQLFDFLGVDQWLR[LGDQW/&06
Acknowledgment: This study was supported by Wroclaw Medical University grant ST-909.

THE EFFECTS OF ORGANIC FERTILIZERS ON

CAROTENOID COMPOUNDS AND YIELD IN SAFFRON
(CROCUS SATIVUS L.)
Majid Jafarimoghadam1, Elham Ghasemi2, Nasim Khalesi2

Tehran Payamnoor University, Department of Agriculture, Iran
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2
In order to assess some physiological and agronomic traits in saffron, a factorial experiment
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DQGWKD5HVXOWVVKRZHGWKDWDOOSDUDPHWHUVZHUHDIIHFWHGE\PDLQHIIHFWVDQGLQWHUDFWLRQV
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PRUHUDWHVRIDOOIHUWLOL]HUVVKRZHGKLJKHUDPRXQWVRISDUDPHWHUV)RULQVWDQFHDSSOLFDWLRQ
of 5 t ha PSMC demonstrated the highest percentage of crocin, picrocrocin and safranal
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Key words:VDIIURQRUJDQLFIHUWLOL]HUVFURFLQSLFURFURFLQVDIUDQDODQGGU\VWLJPD\LHOG

ANTIBACTERIAL ACTIVITY OF DIFFERENT LEAF

EXTRACTS OF CENTAUREA CALCITARPA L. FROM
SERBIA
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0LODQ*DYULORYLü3HGMD-DQDüNRYLü

,Q WKLV ZRUN ZH LQYHVWLJDWHG DQWLPLFURELDO DFWLYLW\ RI PHWKDQROLF 0H2+ HWK\ODFHWLF
(W2$F HWKDQROLF (W2+ DQG DFHWRQLF 0H&2 OHDI H[WUDFWV RI Centaurea
calcitrapa / DJDLQVW EDFWHULDO SK\WRSDWKRJHQLF DQG KXPDQ RSSRUWXQLVWLF DQG SDWKRJHQLF
VWUDLQV0DQ\Centaurea species exhibit different biological activities and are often used in
IRONPHGLFLQH7KHH[WUDFWVZHUHREWDLQHGXVLQJVWDQGDUGPDFHUDWLRQSURFHGXUHDQGPRGL¿HG
diffusion well method, was used as a preliminary screening method for the antimicrobial
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strains [Listeria monocytogenes $7&& 0HWKLFLOOLQUHVLVWDQW Staphylococcus aureus
056$$7&& Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC
6KLJHOODÀH[QHUL$7&&Escherichia coli ATCC 25922, Pseudomonas syringae
pv. aptata33DQG3Xanthomonas arboricola pv. juglandis,=%DQG,=%
Xanthomonas campestris pv. campestrisDQGAgrobacterium tumefaciens@VWUDLQVZHUH
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LQKLELWLRQRUVKRZHGDSRVLWLYHDFWLYLW\,QVHQVLWLYHVWUDLQVZHUHE. coli and both phytopathogenic
strains of X. arboricola pv. juglandis7KHDQWLEDFWHULDOSRWHQWLDORIWKHWHVWHGH[WUDFWVZDVDV
IROORZV0H&2‫(ޒ‬W2+‫ޒ‬0H2+‫(ޒ‬W2$FH[WUDFW7KHEHVWDFWLYLW\ZDVVKRZQE\
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The obtained results showed that C. calcitrapa possesses pronounced antimicrobial activity,
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Key words: Centaurea, leaf extracts, antibacterial activity

ORIGANUM MAJORANA L. AND O. VULGARE L. FROM

SERBIA ARE PROMISING AS FUNCTIONAL FOOD
0DULDQD2DOÿH1$QD$OLPSLü$UDGVNL1, Katarina Šavikin2, Petar D.
Marin16RQMD'XOHWLü/DXãHYLü1

University of Belgrade, Faculty of Biology, Department of Plant Morphology and
6\VWHPDWLFV,QVWLWXWHRI%RWDQ\DQG%RWDQLFDO*DUGHQ³-HYUHPRYDF´7DNRYVND
Belgrade, Serbia
2
Belgrade, Serbia
Genus Origanum/FRPSULVHVVSHFLHVXVHGDVVSLFHPHGLFLQHVIUDJUDQFHDQGRUQDPHQWDO
SODQWV$PRQJWKHPO. majorana /VZHHWPDUMRUDPDQGO. vulgare/RUHJDQRDUHKLJKO\
YDOXHG SODQWV LQ FXOLQDU\ HVSHFLDOO\ LQ WKH 0HGLWHUUDQHDQ 6LQFH OLWHUDWXUH VXUYH\ VKRZHG
versatile biological activities of Origanum VSS WKH DLP RI RXU VWXG\ ZDV WR H[DPLQH WKH
antioxidant and antineurodegenerative activities of O. majorana and O. vulgareIURP6HUELD
The plant material was obtained from the Institute for Medicinal Plant Research “Dr Josif
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The analyses were carried out spectrophotometrically, including the ones of the total phenolic
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were used for the assessment of the antioxidant activity, whereas the antineurodegenerative
activity was evaluated using acetylcholinesterase (AChE) and tyrosinase (TYR) inhibition
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DFWLYLW\WKDQWKRVHRIVZHHWPDUMRUDPDQGWKHDFWLYLWLHVZHUHFRQFHQWUDWLRQGHSHQGHQW$OVR
RUHJDQRDTXHRXVH[WUDFWVVKRZHGDKLJKHUUDWHRIHQ]\PHLQKLELWLRQLQ$&K(DQG
LQ7<5DVVD\DWWKHFRQFHQWUDWLRQRIPJP/) in comparison with sweet marjoram
H[WUDFWV73&DQG7)&ZHUHVWURQJO\FRUUHODWHGDPRQJHDFKRWKHUU DVZHOODVZLWK
DOORIWKHDQWLR[LGDQWDVVD\VU 0RUHRYHUWKHFRUUHODWLRQDPRQJ$&K(DQGWKH
DQWLR[LGDQWDVVD\VZDVZHDNZKLOH7<5DVVD\VKRZHGDPRGHUDWHFRUUHODWLRQZLWKDOODSSOLHG
WHVWVH[FHSWȕFDURWHQHEOHDFKLQJDVVD\7KHJLYHQUHVXOWVSRLQWHGWDUJHWOriganum species as
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Key words: Origanum majorana, Origanum vulgare/DPLDFHDHELRORJLFDODFWLYLWLHV

extracts

ANTIOXIDANT AND ANTINEURODEGENERATIVE

ACTIVITIES OF MELISSA OFFICINALIS L. AND
MENTHA x PIPERITA L. CULTIVATED IN SERBIA
0DULDQD2DOÿH1$QD$OLPSLü$UDGVNL1, Katarina Šavikin2, Sonja
'XOHWLü/DXãHYLü1

University of Belgrade, Faculty of Biology, Department of Plant Morphology and
6\VWHPDWLFV,QVWLWXWHRI%RWDQ\DQG%RWDQLFDO*DUGHQ³-HYUHPRYDF´7DNRYVND
Belgrade, Serbia
2
Belgrade, Serbia
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WKH DQWLR[LGDQW DQG DQWLQHXURGHJHQHUDWLYH SRWHQWLDO RI VDPSOHV IURP 6HUELD &UXGH H[WUDFWV
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antineurodegenerative activity was evaluated using acetylcholinesterase (AChE) and tyrosinase
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indicate that both species can be considered as strong antioxidant and antineurodegenerative
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PHYTOTOXIC ACTIVITY OF AQUEOUS EXTRACTS

FROM DIFFERENT ARTEMISIA ABSINTHIUM L.
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AND BASIL
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evaluation of phytotoxic activities of wormwood on the germination of Lactuca sativa /
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DQGFOLPDWLFFKDPEHUZLWKSURJUDPRI&&NO[OLJKWLQWHQVLW\ZLWKKGD\K
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$TXHRXVextracts of high concentrations of both wormwood chemotypes demonstrated strong
inhibition RQWKHJHUPLQDWLRQRIEDVLODQGOHWWXFH
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OPTIMIZATION OF SUPERCRITICAL FLUID

EXTRACTION OF CARNOSIC ACID FROM SAGE
LEAVES (SALVIA OFFICINALIS L.)
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COMBINATION OF CYTOSTATIC AND ANTIOXIDANT

PRINCIPLES IN LEUKEMIA CELLS TREATMENT
Paraskev Nedialkov1, Yana Ilieva1, Diana Ivanova2, Alexander Tashev3,
Marta Olech4, Renata Nowak4, George Angelov2, Flora Tsvetanova2,
Teodora Atanassova1, Zlatina Kokanova-Nedialkova1

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/XEOLQ3RODQG
The Juniperus sabina / OHDI H[WUDFW VKRZHG D VXSHULRU DQWLFDQFHU DFWLYLW\ RQ 1% DFXWH
SURP\HORF\WLFOHXNHPLDFHOOVFRPSDUHGWRWKHH[WUDFWVRIVRPHRWKHUPHGLFLQDOSODQWVVXFK
as Ruta graveolens/OHDYHVInula britannica/ÀRZHUKHDGVInula helenium/URRWV
Betula pendula5RWKFRUWH[Buxus sempervirens/OHDYHVHWF,QUHVSRQVHWRGHPDQGVRI
the clinical practice in the prevention of harmful action of cytostatic agents on healthy tissues,
we aimed our study at estimating the impact of Rhodiola rosea/*ROGHQURRWH[WUDFWDQ
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cytostatic agents in the presence or absence of Rh. roseaH[WUDFWZHUHDQDO\]HG,&YDOXHV
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in the future clinical trials regarding combination of cytostatic and antioxidant principles
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Science Fund, project “Development of green eco-technologies” of the Bulgarian Academy
of Sciences and ‘Erasmus plus’ agreement (2016-2020) between the Bulgarian Academy of
Sciences and the Medical University of Lublin, Poland.

STANDARDIZED AJUGA GENEVENSIS L. AERIAL

PARTS EXTRACTS WITH ANTITUMOR POTENTIAL
Anca Toiu1, Valentin Florian Rauca2, Manuela Banciu2, Laurian Vlase3,
Ana-Maria Gheldiu3, Alexandru Gâvan3, Ilioara Oniga1

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2
Babes-Bolyai University, Faculty of Biology and Geology, Department of Molecular
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3
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7HFKQRORJ\DQG%LRSKDUPDF\9%DEHV6WUHHW&OXM1DSRFD5RPDQLD
Ajuga genevensis / /DPLDFHDH LV DQ KHUEDFHRXV ÀRZHULQJ VSHFLHV XVHG LQ 5RPDQLDQ
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The anticancer activity of natural extracts is attributed to their synergistically acting complex
PL[WXUH RI SK\WRFKHPLFDOV 7KLV UHVHDUFK DLPHG WR REWDLQ VWDQGDUGL]HG H[WUDFWV IURP A.
genevensis (Ag) aerial parts and to evaluate the cytotoxic potential by testing the effects
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LQ WKH ¿QDO SURGXFW 7KH $J DHULDO SDUWV GULHG H[WUDFW FRXOG EH D SURPLVLQJ LQWHUPHGLDWH
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Key words: Ajuga genevensis, polyphenols, iridoids, antitumor potential
Acknowledgement: This research received funding from UEFISCDI, Romania, project no.
31,,587(

BIOACTIVITY AND CHEMICAL PROFILE STUDY OF

ESSENTIAL OILS FROM VARIOUS MEDICINAL PLANTS
Meriem Bouzeraa-Bessila1, Hayette Bouzeraa2, Nawel Labed3

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antibiotics due to bacterial multiresistance and antibiotherapy failure, we have focused on
the study of three aromatic plants (6DOYLD RI¿FLQDOLV / Lavandula angustifolia 0LOO Ruta
montana /FRPPRQO\XVHGE\WKHORFDOSRSXODWLRQ7KHVWXG\FRQVLVWV¿UVWLQFKDUDFWHUL]LQJ
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hydrodistillation and then in evaluation of their antibacterial and antifungal activities by
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properties, considered interesting in this study, on the tested fungi and Gram (+) and Gram (-)
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some antibiotics multiresistant bacteria and to offer, eventually, possibility of their use in
SKDUPDFHXWLFDODQGDJULIRRGLQGXVWULHV
Key words: antibacterial activity, antifungal activity, 6DOYLDRI¿FLQDOLV, Lavandula

angustifolia, Ruta montana /

EFFECT OF INOCULATION WITH SALT TOLERANT

STRAIN OF RHIZOBIUM ON GROWTH AND
PROTEIN PRODUCTION OF ALFALFA UNDER SALT
CONSTRAINTS
Meriem Bouzeraa-Bessila1, Hayette Bouzeraa2 and Nawel Labed3

/DERUDWRU\RISODQW%LRORJ\DQG(QYLURQPHQW'HSDUWPHQWRI%LRORJ\$QQDED8QLYHUVLW\
Algeria
2
Department of Biology, Tebessa University, Algeria
3
Department of Biology, El- Tarf University, Algeria
Alfalfa or Medicago sativa / LV D VSHFLHVIURPIDPLO\)DEDFHDH,WLVFKDUDFWHUL]HGE\ DQ

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from genus Rhizobium LW FDQ JURZ RQ VRLOV GH¿FLHQW LQ QLWURJHQ ZLWKRXW DSSOLFDWLRQ RI
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renewable production, at lower cost molecules of interest without negative environmental
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In order to promote alfalfa in these areas, the plants inoculation by adapted and compatible
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LQRFXODWLRQPHWKRGLVDQHI¿FLHQWDOWHUQDWLYHDWDOOOHYHOVRIWKHVDOWVWUHVVUHVXOWLQJLQKLJKHU
protein yields and aerial dry biomass when compared to alfalfa plants treated with chemical
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Key words:DOIDOID5KL]RELXPVDOWFRQVWUDLQWVELRPDVVDQGSURWHLQ\LHOGV

IONIC LIQUID-SUPPORTED SOLID–LIQUID

EXTRACTION FOR THE QUANTITATIVE
DETERMINATION OF GALANTAMINE IN LEUCOJUM
AESTIVUM L. (AMARYLLIDACEAE)
Ivan Svinyarov, Rozalina Keremedchieva, Milen G. Bogdanov
)DFXOW\RI&KHPLVWU\DQG3KDUPDF\6R¿D8QLYHUVLW\6W.O2KULGVNL6R¿D%XOJDULD
$TXHRXVVROXWLRQVRIDVHULHVRIK\GURSKLOLFLPLGD]ROLXPS\UUROLGLQLXPDQGDPPRQLXP
EDVHG LRQLF OLTXLGV ZHUH XVHG DV H[WUDFWDQWV IRU WKH HIIHFWLYH H[WUDFWLRQ RI JDODQWDPLQH
from aerial parts of Leucojum aestivum / 7KH LQÀXHQFH RI WKH DQLRQ FKORULGH EURPLGH
DFHVXOIDPDWH WULÀXRURDFHWDWH DFHWDWH WKLRF\DQDWH VDFFKDULQDWH DQG GLFLDQDPLGH FDWLRQ
LPLGD]ROLXPS\UUROLGLQLXPDQGDPPRQLXPZLWKGLIIHUHQWVXEVWLWXHQWVDON\OFKDLQOHQJWKLQ
WKHLPLGD]ROLXPFDWLRQH[WUDFWLRQWLPHSDUWLFOHVVL]HWHPSHUDWXUHFRQFHQWUDWLRQDQGSODQW
PDWHULDOH[WUDFWDQW UDWLR ZDV LQYHVWLJDWHG 2SWLPXP FRQGLWLRQV IRU TXDQWLWDWLYH H[WUDFWLRQ
RI JDODQWDPLQH ZHUH IRXQG7KH GDWD REWDLQHG LQ WKLV VWXG\ UHVXOWHG LQ WKH GHYHORSPHQW RI
an analytical procedure for determination of galantamine in plant material of L. aestivum by
PHDQV RI LWV SUHOLPLQDU\ H[WUDFWLRQ ZLWK DQ DTXHRXV VROXWLRQ RI >&&LP@ &O IROORZHG E\
+3/&TXDQWL¿FDWLRQ7KLVFRXOGEHRIDJUHDWLPSRUWDQFHIURPDQLQGXVWULDOVWDQGSRLQWGXHWR
WKHIDVWHUDQGVDIHUQDWXUHRIWKHSURSRVHGPHWKRG
Key words:JDODQWDPLQHLRQLFOLTXLGVLeucojum aestivumVROLG±OLTXLGH[WUDFWLRQ
$FNQRZOHGJHPHQW7KH¿QDQFLDOVXSSRUWRIWKH1DWLRQDO6FLHQFH)XQGRI%XOJDULDDWWKH
Ministry of Education and Science (project DFNI T 02/23) is greatly acknowledged by the
authors.

PHYTOCHEMICAL EVALUATION AND ASSESSMENT

OF BIOLOGICAL EFFECTS OF AJUGA REPTANS L.
AERIAL PARTS FROM ROMANIA
Ana-Maria Gheldiu1, Anca Toiu2, Laurian Vlase1, Alina Pârvu3, Andrei
Mocan4, Ilioara Oniga2

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RI3KDUPDFHXWLFDO7HFKQRORJ\DQG%LRSKDUPDFHXWLFV9%DEHV6WUHHW&OXM1DSRFD
5RPDQLD
2
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3
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RI3DWKRSK\VLRORJ\9%DEHV6WUHHW&OXM1DSRFD5RPDQLD

RI3KDUPDFHXWLFDO%RWDQ\9%DEHV6WUHHW&OXM1DSRFD5RPDQLD
In Romanian traditional medicine, several Ajuga species are commonly used for their
EHQH¿FLDO HIIHFWV VXFK DV KHSDWRSURWHFWLYH DQWLLQÀDPPDWRU\ DQG ZRXQG KHDOLQJ HIIHFWV
The aim of this study was to investigate Ajuga reptans / HWKDQRO DHULDO SDUWV H[WUDFWV E\
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RI SRO\SKHQROV WRWDO SKHQROLF FRQWHQW DQG WRWDO ÀDYRQRLG FRQWHQW VSHFWURSKRWRPHWULF DQG
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7($&DQG(3)VSHFWURVFRS\DVVD\VZHUHXVHG7KHDQWLLQÀDPPDWRU\HIIHFWRIWKUHHHWKDQRO
H[WUDFWFRQFHQWUDWLRQVZHUHHYDOXDWHGin vivo on a model turpentine oil-
LQGXFHG LQÀDPPDWLRQ LQ UDWV E\ GHWHUPLQLQJ VHYHUDO SDUDPHWHUV :%& FRXQW GLIIHUHQWLDO
WBC count, serum total nitrites and nitrates, total oxidative status, total antioxidant response
DQGR[LGDWLYHVWUHVVLQGH[7KHVHWKUHHH[WUDFWGLOXWLRQVZHUHDOVRHYDOXDWHGin vitro for the
DELOLW\WRLQKLELWSKDJRF\WRVLV&DIIHLFDQGIHUXOLFDFLGVTXHUFLWULQOXWHROLQDQGDSLJHQLQZHUH
LGHQWL¿HG E\ /&06 LQ DOO VDPSOHV +DUSDJLGH 2DFHW\OKDUSDJLGH KDUSDJRVLGH DXFXELQ
DQG FDWDOSRO ZHUH LGHQWL¿HG DQG TXDQWL¿HG LQ DOO H[WUDFWV 7KH PDMRU LULGRLGV FRQVWLWXHQWV
ZHUH 2DFHW\OKDUSDJLGH DQG KDUSDJLGH 7KH GLOXWHG H[WUDFW KDG WKH EHVW LQKLELWRU\
DFWLYLW\RQSKDJRF\WRVLVDQGR[LGDWLYHVWUHVV&RPSDUHGWRGLFORIHQDFWKHAjuga reptans
H[WUDFWVKRZHGEHWWHUDQWLLQÀDPPDWRU\DQGDQWLR[LGDWLYHVWUHVVHIIHFWVDQGWKLVFRXOGMXVWLI\
WKH LPSRUWDQFH RI WKH FRUUHODWLRQ EHWZHHQ WKH DFWLYLW\ DQG WKH GRVH Ajuga reptans extracts
FRQWDLQELRDFWLYHFRPSRXQGVZKLFKSRVVHVVDQWLLQÀDPPDWRU\DQGDQWLR[LGDQWDFWLYLW\WKXV
FRQ¿UPLQJLWVXVHLQWUDGLWLRQDOPHGLFLQHDVDQDQWLLQÀDPPDWRU\DJHQW
Key words: Ajuga reptansSRO\SKHQROVLULGRLGVDQWLR[LGDQWDQWLLQÀDPPDWRU\
Acknowledgement: This research received funding from UEFISCDI, Romania, project no.
31,,587(

SHORT REVIEW OF THE ANTICANCER AND

CYTOTOXIC ACTIVITY OF SOME SPECIES FROM
GENUS EUPHORBIA
Mihail Aleksandrov1, Viktorija Maksimova1, Rubin Gulaboski1, Liljana
Koleva Gudeva2

Goce Delcev University, Faculty of Medical Sciences, Department of Pharmacy, Krste
0LVLUNRY$6WLS5HSXEOLFRI0DFHGRQLD
2
Goce Delcev University, Faculty of Agriculture, Department of Plant Biotechnology, Krste
0LVLUNRY$6WLS5HSXEOLFRI0DFHGRQLD
(XSKRUELDFHDHIDPLO\LVDZLGHO\VSUHDGIDPLO\WKDWFRQWDLQVPRUHWKDQVSHFLHVLQWKH
genus Euphorbia7KHSODQWVRIWKLVJHQXVKDYHEHHQXVHGIRUDORQJWLPHLQWUDGLWLRQDOPHGLFLQH
7KHLU PDLQ DFWLYH FRPSRQHQWV DONDQHV WULWHUSHQHV SK\WRVWHUROV WDQQLQV SRO\SKHQROV DQG
ÀDYDQRLGVDUHVXSSRVHGWREHUHVSRQVLEOHIRUGLIIHUHQWW\SHVRIDFWLYLW\Euphorbia formosana
+D\DWDLVDPHGLFLQDOSODQWXVHGWRWUHDWUKHXPDWLVPOLYHUFLUUKRVLVKHUSHV]RVWHUDORQJDV
WXPRUVXSSUHVVRUEuphorbia tirucalli/KDVEHHQXVHGWRREWDLQPHWKDQROLFH[WUDFWV7KHLU
cytotoxic activity has been examined against many different types of cancer cells, as well as
colon cancer cell line, liver cancer cell line, ovarian cancer cell line and prostate cancer cell
OLQH/HXNHPLFFHOOOLQHV7+3DQG+/KDYHEHHQLQKLELWHGGRVHGHSHQGHQWDIWHUKRI
WUHDWPHQWZLWKȝJP/ Euphorbia formosana+D\DWDIn vitro anticancer activity assays
of Euphorbia formosana+D\DWDVXJJHVWSRWHQWDQWLFDQFHUHIIHFWVWKDWFDXVHERWKFHOOF\FOH
DUUHVWDQGDSRSWRVLVRIOHXNHPLFFDQFHUFHOOV7KHHWKDQROLFH[WUDFWRIEuphorbia helioscopia
/LQKLELWHGWKHJURZWKRIRQO\WKUHHFDQFHUFHOOOLQHV+HS7'DQG3&
&HOO YLDELOLW\ DVVD\V ZHUH FRQGXFWHG RQ WKH SDQFUHDWLF FDQFHU SULPDU\ WXPRU FHOO
line to assess the relative toxicity of the Euphorbia tirucalli/H[WUDFWV7KHWR[LFLW\RIERWK
extracts was found to be dose dependent, with cell viability decreasing with increasing extract
FRQFHQWUDWLRQ%RWKDTXHRXVDQGPHWKDQROH[WUDFWVGHPRQVWUDWHGVLPLODUDFWLYLW\DWȝJP/
ZLWKDYLDELOLW\RIZKLOHRQO\WKHPHWKDQROH[WUDFWH[HUWHGDVLJQL¿FDQWGHFUHDVHLQFHOO
YLDELOLW\IURPȝJP/7KHSURQRXQFHGF\WRWR[LFDFWLYLW\RIWKLVYHU\IHZVSHFLHVIURPWKH
genus Euphorbia, suggest that it could be very interesting to investigate more deeply about
WKHLUSRWHQWDQWLFDQFHUDELOLW\6RWKHDLPRIWKLVVWXG\ZDVWRUHYLHZWKHDQWLFDQFHUDFWLYLW\RI
some of the species examined experimentally by now, using different in vitro or in vivoDVVD\V
Key words: anticancer effects, cytotoxic effects, genus Euphorbia, medicinal plants

IONIC LIQUID-ASSISTED MICELLAR EXTRACTION

FOR THE QUANTITATIVE DETERMINATION OF
SESQUITERPENIC ACIDS IN VALERIANA OFFICINALIS
L. (CAPRIFOLIACEAE)
Ivan Svinyarov, Milen G. Bogdanov
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7KH SHUIRUPDQFH RI DTXHRXV VROXWLRQV RI D VHULHV RI K\GURSKLOLF LRQLF OLTXLGV ,/V LQ WKH
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EURPLGHDFHVXOIDPDWHWULÀXRURDFHWDWHDFHWDWHWKLRF\DQDWHVDFFKDULQDWHDQGGLFLDQDPLGH
FDWLRQ W\SH LPLGD]ROLXP S\UUROLGLQLXP DQG DPPRQLXP DQG VXEVWLWXWLRQ SDWWHUQ
FRQFHQWUDWLRQ H[WUDFWLRQ WLPH WHPSHUDWXUH DQG SODQW PDWHULDOH[WUDFWDQW UDWLR ,W ZDV
IRXQG WKDW VSHFL¿F LQWHUDFWLRQV VXFK DV K\GURJHQ ERQGLQJ LRQGLSROH K\GURSKRELF RU ʌʌ
interactions, do not affect the extraction yields (EY) and that the extraction of acetoxyvalerenic
DQGYDOHUHQLFDFLGVLVVWURQJO\GHSHQGHQWRQWKHDELOLW\RI,/WRIRUPPLFHOOHV7KHSURSRVHG
PLFHOOHEDVHG PHFKDQLVP RI H[WUDFWLRQ ZDV FRQ¿UPHG E\ DGGLWLRQDO H[SHULPHQWV ZLWK
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)XUWKHURSWLPL]DWLRQRIWKHH[WUDFWLRQFRQGLWLRQVEURXJKWDERXWWKHGHYHORSPHQWDQGYDOLGDWLRQ
RIDQDOWHUQDWLYHPHWKRGIRUTXDQWL¿FDWLRQRIVHTXLWHUSHQLFDFLGVLQURRWVRI9RI¿FLQDOLV by
PHDQVRILWVSUHOLPLQDU\H[WUDFWLRQZLWKDQDTXHRXVVROXWLRQRI>&&LP@&OIROORZHGE\
+3/&TXDQWL¿FDWLRQ7KHPHWKRGGHYHORSHGHQVXUHVWKHVDPH(<DVWKHUHIHUHQFHSURFHGXUHV
EXWGRHVQRWUHTXLUHVSHFLDOHTXLSPHQWLWLVHDV\WRSHUIRUPDQGH[FOXGHVWKHKDUPIXORUJDQLF
solvents from the sample preparation step, the latter being of a great importance from an
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Key words:LRQLFOLTXLGVVROLGOLTXLGH[WUDFWLRQYDOHULDQVHVTXLWHUSHQHVDFLGV
$FNQRZOHGJHPHQW7KH¿QDQFLDOVXSSRUWRIWKH1DWLRQDO6FLHQFH)XQGRI%XOJDULDDWWKH
authors.

MICROSTRUCTURE OF THE COMMON SELFHEAL

(PRUNELLA VULGARIS L.) NUTLETS AND MUCILAGE
ENVELOPE
Anely Nedelcheva1, Venelina Angelkova2, Ivan Svinyarov2, Milen G.
Bogdanov2

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6R¿D%XOJDULD
2
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%RXUFKLHU%OYG6R¿D%XOJDULD
Common selfheal (Prunellae spica3K(XUKDVIRXUEURZQRYRLGQXWOHWVWKDWDUHUHWDLQHGLQ

WKHSHUVLVWHQWFDO\[7KHVHQXWOHWVDUHFKDUDFWHUL]HGZLWKSUHVHQFHRIP\[RFDUS\DQGSURGXFH
PXFLODJHZKHQWKHQXWOHWVDUHK\GUDWHG6XFKK\GUDWLRQRFFXUVZKHQWKHKHUEDOVXEVWDQFHLV
SUHSDUHGDVDQLQIXVLRQRUGHFRFWLRQ7KLVVXJJHVWVWKDWSDUWRIWKHKHDOLQJDFWLRQRIKHUEDO
preparations is due to the composition of the nutlets and their mucilage envelope, which
GHWHUPLQHV WKH QHHG IRU WKHLU GHWDLOHG VWXG\ 7KH IROORZLQJ PLFURVWUXFWXUDO IHDWXUHV RI WKH
nutlets were studied by light and scanning electron microscopy: (i) morphology of dry nutlets
VXUIDFHZLWKRXWOLQHVRIWKHPXFLODJHVHFUHWLQJFHOOVWKLFNOD\HUVRIGHSRVLWHGPXFLODJHRI
the mucilage secreting cells) - pericarp composed of mucilage rectangular secreting cells
DFFRPSDQLHGE\SRO\JRQDOFHOOVZLWKWKLFNHQHGZDOOVDQGJUDQXODUFRQWHQWVLLPRUSKRORJ\
RIK\GUDWHGDQGODWHO\GULHGQXWOHWVWKLQ¿OPVXUURXQGLQJWKHQXWOHWVDQGPHPEUDQHVREVHUYHG
FRUUHVSRQGWRGU\PXFLODJHRQWKHVXUIDFH±KRPRJHQRXVVPRRWKVWUXFWXUHVRPHZULQNOHV
DQGIUDFWXUHVRIWKHPXFLODJLQRXVPDWHULDOFHOOXORVH¿EULOVDUHDUUDQJHGZLWKLQWKHPXFLODJH
8SRQ IXOO K\GUDWLRQ ¿ODPHQWV EHFDPH DSSDUHQW DQG FRQIRUPHG WR D WUDQVSDUHQW µ¶FDSVXOH¶¶
DWWDFKHG WR WKH QXWOHW 7KH JHQHUDO PRUSKRORJ\ RI QXWOHW GHWHUPLQHV WKH VSHFL¿FLW\ RI WKH
process and three main stages were outlined: initial mucilage developing in white hilum at
QDUURZDQGFXWHHQGIRUPDPXFLODJHDURXQGGLVWLQFWLYHGDUNOLQHVUXQGRZQFHQWUHRIHDFK
QXWOHWIDFHDQGDURXQGVWLIIJHODWLQRXVHQYHORSHRYHUWKHQXWOHWVXUIDFHZLWKDGLVWLQFWRXWHU
ERXQGDU\7KH VWXG\ UHSRUWV WKH FRPSDUDWLYH HIIHFW RI ZDWHU DQG LRQLF OLTXLGV IRU PXFLODJH
GHYHORSLQJSURFHVV
Key words: common selfheal, Prunella vulgarisPXFLODJHP\[RFDUS\LRQLFOLTXLGVQXWOHWV

that are retained

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authors.

INFLUENCE OF PLANT AGE ON THE CONTENT AND

COMPOSITION OF ESSENTIAL OIL OF FENNEL SEEDS
Crop Reseach Institute, Division of Crop Genetics and Breeding, Genetic Resources of
9HJHWDEOHVDQG6SHFLDOW\&URSVâOHFKWLWHOĤ2ORPRXF
Fennel (Foeniculum vulgare0LOOLVDZHOONQRZQDQGRIWHQFXOWLYDWHGPHGLFLQDODQGDURPDWLF

SODQW$FFRUGLQJWRYDULRXVFOLPDWLFFRQGLWLRQVLWFDQEHJURZQDVRQHWRWKUHH\HDUFURS7KH
HIIHFWRISODQWDJHRQWKHHVVHQWLDORLO(2FRQWHQWDQGFRPSRVLWLRQZDVWKHVXEMHFWRIWKLV
VWXG\7KHVHHGVRIJHQRW\SHVIURPWKH&]HFKFROOHFWLRQRISODQWJHQHWLFUHVRXUFHVZHUH
HYDOXDWHG LQ WZR VHDVRQV DQG DV D RQH WZR DQG WKUHH\HDUV ROG SODQWV 7KH
HYDOXDWHG IHQQHO DFFHVVLRQV RULJLQDWHG IURP WKH &]HFK 5HSXEOLF RU IRUPHU &]HFKRVORYDNLD
DVZHOODVIURP*HUPDQ\+XQJDU\WKH1HWKHUODQGV3RODQGDQG6ORYHQLD7KH(2FRQWHQW
ZDVDQDO\]HGE\K\GURGLVWLOODWLRQDQGWKHFRQWHQWRIWKHPDMRU(2FRPSRQHQWVE\*&06
DFFRUGLQJWR3KDUPDFRSRHD%RKHPLFD$OOWKHDVFHUWDLQHGYDOXHVRI(2FRPSRQHQWVFRQWHQW
are expressed as the average (Av) of all evaluated genotypes, plant ages and vegetative seasons
LWVVWDQGDUGGHYLDWLRQ7KHWZRZD\DQDO\VLVRIYDULDQFHZLWKRXWUHSOLFDWLRQZDVXVHGIRU
WKHVWDWLVWLFDOHYDOXDWLRQ:HIRXQGRXWWKDWWKHWHVWHGJHQRW\SHVSURGXFHGLQDYHUDJHRIWZR
VHDVRQVDQGWKUHHGLIIHUHQWFURSDJHV±RI(2DQGWKHKLJKHVW(2\LHOG
ZDVGLVFRYHUHGLQWZR\HDUVROGFURS7KHVWDWLVWLFDOO\VLJQL¿FDQWGLIIHUHQFHVȜ LQ(2
content were found between the tested genotypes as well as between the plants of different
DJHZKLOHVXUSULVLQJO\QRVWDWLVWLFDOO\VLJQL¿FDQWGLIIHUHQFHȜ ZDVIRXQGEHWZHHQWKH
YHJHWDWLRQVHDVRQV7KHFRQWHQWRIWUDQVDQHWKROWKHPDMRUFRPSRXQGRIIHQQHO(2ZDVIRXQG
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IDFWRUZKLFKVWDWLVWLFDOO\Ȝ LQÀXHQFHGLWVFRQWHQW$OVRFRQWHQWRIHVWUDJRO$Y
DQG IHQFKRO $Y ZHUH VWDWLVWLFDOO\ Ȝ LQÀXHQFHG RQO\ E\
JHQRW\SHVZKLOHWKHFRQWHQWRIȕSLQHQH$YDQGȕP\UFHQH
ZHUHLQÀXHQFHGE\JHQRW\SHȜ RUDVZHOODVE\WKHYHJHWDWLYHVHDVRQȜ
$FFRUGLQJWRRXUUHVXOWVWKH(2FRQWHQWZDVWKHRQO\FKDUDFWHULVWLFLQÀXHQFHGE\GLIIHUHQWDJH
RIIHQQHOSODQWVZKLOHWKH(2FRPSRVLWLRQUHPDLQHGVWDEOH2IFRXUVHWKHKXJHGLIIHUHQFHVFDQ
be expected in the yield of plant biomass per unit of area, because the 2-year old plants were
PRVWO\REVHUYHGKLJKHUDQGJHQHUDOO\PRUHUREXVWZKHQFRPSDUHGWR\RXQJHVWSODQWV2QWKH
RWKHUKDQGVRPHJHQRW\SHVZHUHKDQGLFDSSHGE\WKH¿UVWDQGHVSHFLDOO\E\WKHVHFRQGEDG
ZLQWHULQJ7KH&]HFKRULJLQYDULHW\0RUDYVNêVHHPHGWKHEHVWDGDSWHGWRWKH&]HFKFOLPDWLF
DQGVRLOFRQGLWLRQDOWKRXJKWKHVHUHVXOWVDUHEDVHGRQO\RQREVHUYDWLRQV8QIRUWXQDWHO\WKH
VHHG\LHOGSHUXQLWRIDUHDZDVQRWZHLJKHG
Key words: Foeniculum vulgare, essential oil, plant age, trans-anethol

EFFECT OF ARTEMISIA ABSINTHIUM L. EXTRACT

AGAINST FASCIOLA HEPATICA LARVAE
Katya Georgieva1, Veselin Nanev2, Nely Tsocheva-Gaytandzhieva2, Ina
Aneva1, Strahil Berkov1, Milena Nikolova1

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2
Institute of Experimental Morphology, Pathology and Anthropology with Museum,
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Fasciola hepatica is a helminth parasite affecting wild and domestic animals and humans
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GHYHORSPHQW RI WKUHH ODUYDO VWDJHV WDNHV SODFH ± VSRURF\VWV UHGLDH DQG FHUFDULDH 7KH
VXSSUHVVLRQ RI WKH LQWUDVQDLO GHYHORSPHQW RI WKH ODUYDH LV DQ RSSRUWXQLW\ WR EUHDN WKH OLIH
F\FOHRISDUDVLWHRQWKHLQWHUPHGLDWHKRVWOHYHO5HFHQWO\DWWHPSWVDUHEHLQJPDGHWRLGHQWLI\
QDWXUDOO\ RFFXUULQJ SODQWV KDYLQJ VHOHFWLYHO\ ODUYLFLGDO DFWLRQ 7KLV VWXG\ ZDV FRQGXFWHG
to evaluate the effect of methanolic extract from aerial parts of Artemisia absinthium/RQ
the vitality of F. hepatica sporocysts, rediae and cercariae, incubated in physiological saline
for Galba truncatula $PRQJ WKH DSSOLHG FRQFHQWUDWLRQV WKRVH KLJKHU WKDQ PJ /
have a negative effect on the vitality of the larval population after 2 hours of experiment
ZLWK D GHOD\ UDWH H[FHHGLQJ 0HWDEROLF SUR¿OH RI Artemisia absinthium extract was
analysed by gas chromatography-mass spectrometry (GC-MS) and high-performance thin-
OD\HUFKURPDWRJUDSK\+37/&&RPSRXQGVEHORQJLQJWRIDWW\RUJDQLFDQGSKHQROLFDFLGV
IDWW\DOFRKROVÀDYRQRLGVFDUERK\GUDWHVDQGRWKHUZHUHLGHQWL¿HG%DVHGRQWKHUHVXOWVWKH
extract from A. absinthium gave potent larvacidal activity against the F. hepatica larval stages,
DVVRFLDWHGZLWKLWVVQDLOKRVW
Key words:ODUYDFLGDODFWLYLW\*&06PHWDEROLWHSUR¿OH

VERONICA CYMBALARIA Bodard – WHEN THE GC-MS

PEAKS OF VOLATILE COMPOUNDS APPEAR
0DULMD1D]OLü1, Dario Kremer20LUNR5XãþLü11DGD%H]Lü1, Valerija
'XQNLü1

8QLYHUVLW\RI6SOLW)DFXOW\RI6FLHQFH'HSDUWPHQWRI%LRORJ\5XÿHUD%RãNRYLüD+5
6SOLW&URDWLD
2
)DFXOW\RI3KDUPDF\DQG%LRFKHPLVWU\8QLYHUVLW\RI=DJUHE$.RYDþLüD+5
Zagreb, Croatia
Genus Veronica/EHORQJVWRWKH3ODQWDJLQDFHDHIDPLO\DQGDFFRUGLQJWRPRUSKRORJLFDOWUDLWV
LWLVGLYLGHGLQWRVXEJHQXVHV,Q&URDWLDSODQWVSHFLHVRIJHQXVVeronica are spread on a
YDULHW\RIKDELWDWVIURPDTXDWLFPDUVK\IRUHVWFDUERQDWHPRXQWDLQSDVWXUHVDQGPRXQWDLQ
OLPHVWRQHFDYHVWRURFNDQGURFNFUDFNV¿HOGVDQGUXGHUDOKDELWDWV7KHLQYHVWLJDWHGVSHFLHV
Veronica cymbalaria %RGDUG EHORQJV WR 6RXWK(XURSHDQ0HGLWHUUDQHDQ WHURSK\WHV 3ODQW
PDWHULDOZDVFROOHFWHGIURP6SOLW&URDWLDLQWKHVSULQJ$SULO:DWHUGLVWLOOHGHVVHQWLDO
RLOVIURPDHULDOSDUWVRILQYHVWLJDWLRQSODQWKDYHEHHQDQDO\VHGE\*&DQG*&06XVLQJ9)
PVFDSLOODU\FROXPQDQGWKH¿UVWSHDNVDSSHDUDIWHUPLQXWHV7KHWRWDO\LHOGRIRLOZDV
EDVHGRQGU\ZHLJKWRIVDPSOHV7HQFRPSRXQGVZHUHGHWHUPLQHGUHSUHVHQWLQJRI
WKHWRWDORLO&RPSRVLWLRQRIWKLVHVVHQWLDORLOLVFKDUDFWHUL]HGE\DKLJKFRQFHQWUDWLRQRISK\WRO
VHYHUDOGLIIHUHQWK\GURFDUERQVLQWRWDODQGFDU\RSK\OOHQHR[LGH,Q
RXUSUHYLRXVUHVHDUFKSK\WROZDVDOVRLGHQWL¿HGLQWKHRLORIV. spicata,QRXUUHYLHZZHIRXQG
one more species, Veronica thymoides3+'DYLVVXEVSpseudocinerea0$)LVFKHUWKDWZDV
LQYHVWLJDWHGZLWKWKHVDPHPHWKRGRORJ\&RPSRXQGSHDNVIRUV. thymoides also appeared after
PLQXWHV RI WKH *&06 DQDO\VLV DQG WKH PRVW DEXQGDQW FRQVWLWXHQW ZDV KH[DWULDFRQWHQH
7KH SUHVHQW VWXG\ JLYHV DGGLWLRQDO NQRZOHGJH DERXW FKHPRV\VWHPDWLFSUR¿OH RI WKH
genus Veronica
Key words: Veronica, secondary metabolites, GC-MS, phytochemical

LC-HRMS PROFILING OF SOME STEROID

ALKALOIDS FROM LEAVES EXTRACT OF SOLANUM
SCHIMPERIANUM Hochst.
Yulian Voynikov1, Paraskev Nedyalkov2, Dimitrina Zheleva-Dimitrova1,
Vessela Balabanova2, Reneta Gevrenova2

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2
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7KH6RODQDFHDHIDPLO\DUHZHOONQRZQIRUWKHSURGXFWLRQRIQXPHURXVVHFRQGDU\PHWDEROLWHV
DPRQJZKLFKVWHURLGDOJO\FRVLGHVDUHRQHPDMRUFODVV+HUHLQWKHSUR¿OLQJRIWKLVFODVVRI
semipolar metabolites along with approaches for their isolation from complex plant extracts
DUH GLVFXVVHG %DVHG RQ KLJKUHVROXWLRQ DFFXUDWH PDVV PHDVXUHPHQWV DQG FKDUDFWHULVWLF
fragmentation patterns we were able to tentatively identify and differentiate several solanidine-
W\SHDQGVRODVRGLQHW\SHDONDORLGVWKDWPDLQO\GLIIHULQWKHLUVLGHFKDLQF\FOLFIRUVRODQLGLQH
W\SHDQGRSHQFKDLQIRUVRODVRGLQHW\SHDONDORLGV6XFKFRPSRXQGVLQFOXGHYDULRXVQLWURJHQ
FRQWDLQLQJ SHQWDF\FOLF VWHURLG DONDORLGV OLNH VRODQRSXEDPLQH VRODQRFDSVLQH VRODVRGLQH
UXELMHUYLQHDQGRWKHUGHULYDWLYHV0DVVDQDO\VHVZHUHFDUULHGRXWRQD4([DFWLYH3OXVPDVV
VSHFWURPHWHU7KHUPR)LVKHU6FLHQWL¿FHTXLSSHGZLWKDKHDWHGHOHFWURVSUD\LRQL]DWLRQ+(6,
,,SUREH7KHUPR6FLHQWL¿FDQGWKHWDUJHWHGDFTXLVLWLRQZHUHFDUULHGRXWRQSDUDOOHOUHDFWLRQ
PRQLWRULQJ350PRGH
Key words: SolanumJO\FRDONDORLG/&06

PHYTOCHEMICAL ANALYSIS AND ANTIRADICAL

POTENTIAL OF METHANOL EXTRACTS OF
HIERACIUM NAEGELIANUM3DQþLü$1'H.
SCHEPPIGIANUM Freyn UNDERGROUND PARTS
9LROHWD0LOXWLQRYLü16LOYDQD3HWURYLü10DUMDQ1LNHWLü2, Aleksej
.UXQLü3'HMDQ1LNROLü3

University of Belgrade-Faculty of Pharmacy, Department of Pharmacognosy,
9RMYRGH6WHSH%HOJUDGH6HUELD
2
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University of Illinois, College of Pharmacy, Department of Medicinal Chemistry and
3KDUPDFRJQRV\&KLFDJR,/86$
The genus Hieracium/LVDXWRFKWKRQRXVLQ(XUDVLD1RUWK$IULFDDQG1RUWK$PHULFDDQGWKH

YDVWPDMRULW\RIWKHWD[DDUHGLVWULEXWHGLQ(XURSHHieracium naegelianum 3DQþLüEHORQJLQJ
WRPRQRW\SLFVXEHQGHPLFVHFWLRQ+VHFW1DHJHOLDQD=DKQH[6]HOąJLVDVPDOOFDHVSLWRVH
SODQW ZLWK ORQJ UKL]RPH ,W LQKDELWV VXEDOSLQH DQG DOSLQH RURVXEPHGLWHUUDQHDQ VFUHHV DQG
PDLQO\LVHQGHPLFIRUWKHPRXQWDLQVLQ:%DONDQZLWKDVLQJOHSRSXODWLRQLQ6$SHQQLQHV
Hieracium scheppigianum Freyn is a hybridogenous apomictic species probably originated
from H. gymnocephalum*ULVHEH[3DQW+VHFW3DQQRVD=DKQ=DKQDQGH. bupleuroides
&&*PHOLQVO+VHFW'UHSDQRLGHD0RQQLHU7KHVSHFLHVFDQEHIRXQGRQO\RQVFUHHV
URFN\SODFHVRUURFN\SDVWXUHVLQVXEDOSLQHDQGDOSLQH]RQHLQ'LQDULGHV7KHXQGHUJURXQG
parts of H. naegelianum and H. scheppigianum were collected on Mt Durmitor, Republic of
0RQWHQHJUR 'ULHG DQG SRZHUHG SODQW PDWHULDO ZDV PDFHUDWHG ZLWK GLFKORURPHWKDQH DQG
PHWKDQRO VXFFHVVLYHO\ %RWK VROYHQWV ZHUH HYDSRUDWHG XQGHU UHGXFHG SUHVVXUH DQG GULHG
PHWKDQROH[WUDFWVZHUHXVHGIRUIXUWKHULQYHVWLJDWLRQ8VLQJ/&06PHWKRGVHVTXLWHUSHQH
ODFWRQHJO\FRVLGHRIJXDLDQROLGHW\SHFUHSLVLGH(DQGVL[SKHQROLFDFLGVLHFKORURJHQLFDFLG
DQG¿YHGLFDIIHR\OTXLQLFDFLGVF\QDULQDQGGLFDIIHR\OTXLQLFDFLGVZHUH
LGHQWL¿HGDQGTXDQWL¿HGLQERWKGULHGPHWKDQROH[WUDFWV&UHSLVLGH(ZDVWKHPRVWDEXQGDQW
FRPSRXQG DQG PJJ ZKLOH DPRQJ SKHQROLF DFLGV GLFDIIHR\OTXLQLF
DFLGDQGPJJZDVGRPLQDQWLQERWKLQYHVWLJDWHGH[WUDFWV5DGLFDOVFDYHQJLQJ
DFWLYLW\ ZDV HVWLPDWHG XVLQJ FRORULPHWULF '33+ DQG 2+ UDGLFDO DVVD\V DQG ERWK H[WUDFWV
VKRZHG SURPLQHQW DQG FRQFHQWUDWLRQ GHSHQGHQW DFWLYLW\ ZLWK 6&'33+ RI DQG
JP/DQG6&2+RIDQGJP/UHVSHFWLYHO\7KLVLVWKH¿UVWUHSRUWRQ
secondary metabolites and antiradical potential of underground parts of H. naegelianum and
H. scheppigianum
Key words: HieraciumXQGHUJURXQGSDUWVSKHQROLFDFLGVVHVTXLWHUSHQHODFWRQHDQWLUDGLFDO

ANTIOXIDANT, CYTOTOXIC,
ACETYLCHOLINESTERASE AND TYROSINASE
ENZYME INHIBITORY ACTIVITIES OF SIX INULA
SPECIES FROM BULGARIA
$QWRDQHWD7UHQGD¿ORYD1, Victoria Ivanova1, Gulmira Ozek2, Suleyman
Yur2,3, Temel Ozek2,3, Ina Aneva4, Ralitza Veleva5, Tanya Topouzova-
Hristova5, Jordan Doumanov5, Milka Todorova1

2
'HSDUWPHQWRI3KDUPDFRJQRV\)DFXOW\RI3KDUPDF\$QDGROX8QLYHUVLW\(VNLúHKLU
3
0HGLFLQDO3ODQW'UXJDQG6FLHQWL¿F5HVHDUFK&HQWHU$8%,%$0$QDGROX8QLYHUVLW\
(VNLúHKLU7XUNH\

,QVWLWXWHRI%LRGLYHUVLW\DQG(FRV\VWHP5HVHDUFK%XOJDULDQ$FDGHP\RI6FLHQFHV
6R¿D%XOJDULD
5
)DFXOW\RI%LRORJ\6R¿D8QLYHUVLW\?´6W.OLPHQW2KULGVNL?´6R¿D%XOJDULD
Continuing our research on Inula species, growing in Bulgaria we have focused our attention on
SKHQROLFFRQVWLWXHQWVDQGDQWLR[LGDQWF\WRWR[LFDQGHQ]\PHLQKLELWRU\DFWLYLW\RIPHWKDQROH[WUDFWV
of six Inula species - I. aschersoniana -DQND I. conyza '& I. ensifolia / I. oculus-christi /
I. bifrons//DQGI. germanica /+37/&ZDVXVHGIRUTXDOLWDWLYHDQDO\VLVRISKHQROLFDFLGV
7RWDO SKHQROLF FRQWHQW ZDV GHWHUPLQHG E\ )ROLQ±&LRFDOWHX PHWKRG 7KH IUHH UDGLFDO VFDYHQJLQJ
SRWHQWLDO ZDV DVVHVVHG DJDLQVW '33+ and ABTS UDGLFDOV $FHW\OFKROLQHVWHUDVH LQKLELWLRQ ZDV
HYDOXDWHGXVLQJ(OOPDQ¶VPHWKRGZKLOHW\URVLQDVHLQKLELWLRQE\WKHPLFURWLWHUSODWHDVVD\UHSRUWHG
E\0DVXGD7KHF\WRWR[LFDFWLYLW\WRJP/) and analysis of cell morphology were
GHWHUPLQHGE\FU\VWDOYLROHWDVVD\2QO\WKHI. oculus-christi and I. aschersoniana exstracts reached
,&IRUWKHPDOLJQDQWFHOOV$RQKZKLOHYLDELOLW\ZDVDURXQGIRUWKHQRQFDQFHU
RQHV0'&.ȱȱ+37/&FRPSDULVRQRIWKHPHWKDQROH[WUDFWVZLWKFRPPHUFLDOVWDQGDUGVVKRZHG
SUHVHQFHRIFKORURJHQLFDQGGLFDIIHR\OTXLQLFDFLGVI. ensifolia, I. conyza and I.
germanicaPJ*$(J(UHVSFRQWDLQHGWKHKLJKHVW
DPRXQWVRISKHQROLFVDQGGHPRQVWUDWHGWKHKLJKHVWSHUFHQWDJHRILQKLELWLRQRI'33+UDGLFDOVDW
FRQFHQWUDWLRQRIJP/DQGUHVSHFWLYHO\I. bifrons
and I. germanica extracts were found to be the most active Inula VDPSOHVZLWK7($&YDOXHV
DQGPJP/UHVSHFWLYHO\7KHVHWZRVDPSOHVDOVRVKRZHGWKHEHVWLQKLELWRU\
SRWHQWLDODJDLQVWW\URVLQDVHHQ]\PH,&DQGPJP/UHVS$OOWHVWHG
H[WUDFWVGHPRQVWUDWHGZHDNDQWL$&K(DFWLYLW\,QK$JRRGFRUUHODWLRQEHWZHHQWRWDO
SKHQROLFFRQWHQWDQGDQWLR[LGDQWDFHW\OFKROLQHVWHUDVHDQGW\URVLQDVHHQ]\PHLQKLELWRU\DFWLYLWLHV
ZDVREVHUYHGE\FDOFXODWLQJ3HDUVRQ¶VFRUUHODWLRQFRHI¿FLHQWV
Key words: Inula, Asteraceae, phenolic acids, biological activity

DETERMINATION OF THE TOTAL PHENOLIC

CONTENT, ANTIOXIDANT ACTIVITY AND
CYTOTOXICITY OF SELECTED AROMATIC HERBS
Blagica Jovanova1, Svetlana Kulevanova2, Tatjana Kadifkova
Panovska1

6V&\ULODQG0HWKRGLXV8QLYHUVLW\)DFXOW\RI3KDUPDF\,QVWLWXWHRI$SSOLHG%LRFKHPLVWU\
0DMND7HUH]D6NRSMH5HSXEOLFRI0DFHGRQLD
2
6V&\ULODQG0HWKRGLXV8QLYHUVLW\)DFXOW\RI3KDUPDF\,QVWLWXWHRI3KDUPDFRJQRV\
0DMND7HUH]D6NRSMH5HSXEOLFRI0DFHGRQLD
Aromatic plants used as culinary herbs contain wide range of phytochemicals with distinct
ELRDFWLYH SURSHUWLHV DIIHFWLQJ WKH SRSXODWLRQ WKDW XWLOL]HV WKHP +RZHYHU WKHUH LV VWLOO
LQVXI¿FLHQWGDWDRQWKHLUDQWLR[LGDQWFDSDFLW\DQGF\WRWR[LFLW\7KHSUHVHQWVWXG\LQYHVWLJDWHG
WKHDQWLR[LGDQWSURSHUWLHVDQGF\WRWR[LFSRWHQWLDORI¿YHDURPDWLFKHUEVAllium schoenoprasum
/$635Allium ursinum/$85Anthriscus cerefolium/$&+Capsicum annuum
/ &$) DQG Foeniculum vulgare 0LOO )9+ )ROLQ&LRFDOWHX PHWKRG DQG$O&O PHWKRG
ZHUHSHUIRUPHGWRGHWHUPLQHWRWDOSKHQROLFFRQWHQW73&DQGWRWDOÀDYRQRLGFRQWHQW7)&
$QWLR[LGDQW DFWLYLW\ RI HWKDQRO H[WUDFWV ZDV H[DPLQHG E\ ¶GLSKHQ\OS\FU\OK\GUD]\O
'33+ )HUULF UHGXFLQJ DQWLR[LGDQW SRZHU )5$3 1RQVLWHVSHFL¿F 1662+ DQG 6LWH
VSHFL¿FGHR[\ULERVHGHJUDGDWLRQ662+DVVD\V7KHF\WRWR[LFSRWHQWLDORIZDWHUHWKDQRO
H[WUDFWVZDVHYDOXDWHGE\%ULQHVKULPSOHWKDOLW\DVVD\%6/$&RQVLGHUDEOHYDULDWLRQVZHUH
REVHUYHGDPRQJ73&YDOXHVIURPWRPJ*$(J crude extract and among the
7)&YDOXHVIURPWRPJ4(JFUXGHH[WUDFW+LJKHVWTXDQWLW\RIWRWDOÀDYRQRLGVZDV
PHDVXUHGLQ&$)ZKLFKLVLQDFFRUGDQFHZLWKWKHKLJKDPRXQWRIWRWDOSRO\SKHQROV$FFRUGLQJ
WKH '33+ DVVD\ KLJKHVW UDGLFDO VFDYHQJLQJ DFWLYLW\ ZDV REVHUYHG IRU &$) ZKLOH $&+
VKRZHG ORZHVW DQWLR[LGDQW DFWLYLW\ 0RUHRYHU WKH )5$3 FDSDFLW\ LV GHFUHDVLQJ DV IROORZV
&$)!$85!)9+!$635!$&+$GGLWLRQDOO\1662+DQG662+DVVD\VLQGLFDWHGWKDW
&$)KDVSURPLQHQWK\GUR[\OVFDYHQJLQJDQGFKHODWLQJSURSHUWLHV2EWDLQHG/&YDOXHVE\
%6/$UHYHDOHGGLIIHUHQWF\WRWR[LFLW\DPRQJWKHH[WUDFWV$FFRUGLQJ&ODUNVRQ¶VVFDOHVWURQJ
F\WRWR[LFLW\ ZDV REVHUYHG IRU &$) PRGHUDWH F\WRWR[LF HIIHFWV IRU )9+ DQG$635 ZHDN
F\WRWR[LFLW\GHPRQVWUDWHG$85ZKLOH$&+FDXVHGQRF\WRWR[LFHIIHFWVDJDLQVWWKHVKULPSV
The obtained data in the present study indicates that all extracts possess notable antioxidant
SURSHUWLHVDQGF\WRWR[LFSRWHQWLDO'XHWRWKHLUVLJQL¿FDQWELRDFWLYLW\WKHH[DPLQHGVSHFLHV
present promising dietary sources in prevention of pathological conditions associated with
DFFXPXODWLRQRIIUHHUDGLFDOV
Key words: dietary antioxidants, antioxidant capacity, cytotoxic potential, brine shrimp
OHWKDOLW\DVVD\/&

NMR-BASED METABOLOMICS OF VERBASCUM

PURPUREUM (Janka) Hub.-Mor. AND THEIR
TRANSFORMED ROOTS
Andrey S. Marchev1, Darina T. Yazova2, Ivanka K. Koycheva1,
Madeleine Neumann3-XWWD/XGZLJ0OOHU3, Milen I. Georgiev1

Group of Plant Cell Biotechnology and Metabolomics, The Stephan Angeloff Institute of
0LFURELRORJ\%XOJDULDQ$FDGHP\RI6FLHQFHV5XVNL%OYG3ORYGLY%XOJDULD
2
)DFXOW\RI%LRORJ\6R¿D8QLYHUVLW\?´6W.OLPHQW2KULGVNL?´.R]LDN6WU6R¿D
Bulgaria
3
,QVWLWXWHRI%RWDQ\7HFKQLVFKH8QLYHUVLWlW'UHVGHQ=HOOHVFKHU:HJE'UHVGHQ
Germany
The genus Verbascum / PXOOHLQV FRPSULVHV RI DERXW VSHFLHV RI ÀRZHULQJ SODQWV
ZLWKLQWKH6FURSKXODULDFHDHIDPLO\0XOOHLQVKDYHEHHQXVHGLQWKHWUDGLWLRQDOIRONPHGLFLQH
for treatment of a wide range of human ailments, inter alia bronchitis, tuberculosis, asthma,
DQG VHYHUDO RWKHUV LQÀDPPDWLRQ SURFHVVHV 7KH DLP RI WKH VWXG\ ZDV WR GHWHUPLQH WKH
metabolic differences of V. purpureum-DQND+XE0RUPRWKHUSODQWDQGVRQLFDWLRQDVVLVWHG
Agrobacterium rhizogenes-mediated genetic transformation (SAArT) induced hairy roots by
FRPELQLQJ105EDVHGPHWDERORPLFV'DQG'DSSURDFKDQGPXOWLYDULDWHGDWDDQDO\VLV
7KLVLVWKH¿UVWUHSRUWRQWKHJHQHWLFWUDQVIRUPDWLRQRIV. purpureum plants with the application
of metabolimics approach to observe the metabolome alterations as a result of the foreign
7'1$ WUDQVIHU 7KH JHQHWLF WUDQVIRUPDWLRQ RI WZR VHOHFWHG IDVW JURZLQJ KDLU\ URRW OLQHV
GHQRWHGDV93'DQG93'ZDVFRQ¿UPHGE\3&5DQDO\VLV7KHPHWDEROLFDQDO\VLVRIA.
rhizogenesKDVEHHQSHUIRUPHGIRU¿UVWWLPHDVZHOODQGLWZDVIRXQGWRFRQWDLQRQO\SULPDU\
PHWDEROLWHVVXFKDVVXJDUVDQGDPLQRDFLGV7KHPRVWGLVWLQJXLVKHGIHDWXUHRIWKHWUDQVIRUPHG
URRWOLQHVZDVWKHLGHQWL¿FDWLRQRIYHUEDVFRVLGHDSKHQ\OHWKDQRLGJO\FRVLGHZLWKLPSRUWDQW
DQWLLQÀDPPDWRU\DQWLWXPRULJHQLFDQGLPPXQHVWLPXODWLQJDFWLYLWLHV7KHSUHVHQFHRIVRPH
SULPDU\PHWDEROLWHVLQFOXGLQJDPLQRDFLGVRUJDQLFDFLGVDQGVXJDUVZDVUHSRUWHGDVZHOO
The results reported here revealed that the hairy roots from V. purpureum have the potential
to be used as an alternative source for biosynthesis of biologically active molecules, such as
YHUEDVFRVLGH
Key words: Verbascum purpureum/KDLU\URRWV105Agrobacterium rhizogenes,

Verbascoside
Acknowledgement: This work has been supported by a grant from NSF of Bulgaria and
'$$'*HUPDQ\FRQWUDFWQXPEHU'176*HUPDQ\

ANTIOXIDATIVE ACTIVITY OF LEAF EXTRACTS

OF ACHILLEA COARCTATA Poir. AND HIERACIUM
WALDSTEINII Tausch FROM MACEDONIA
3HGMD-DQDüNRYLü-HOLFD1RYDNRYLü0LODQ*DYULORYLü1HPDQMD
5DMþHYLü3HWDU'0DULQ

In this study the antioxidative capacity of leaf extracts of two potentially medicinal plants:
Achillea coarctata3RLUDQGHieracium waldsteinii7DXVFKIURP0DFHGRQLDZHUHLQYHVWLJDWHG
,WLVZHOONQRZQWKDWAchillea/DQGHieracium/VSHFLHVDUHZLGHO\XVHGLQIRONPHGLFLQH
The extracts were obtained using Soxhlet type apparatus, with two different solvents, ethyl-
DFHWDWHDQGPHWKDQRO7KHDQWLR[LGDWLYHFDSDFLW\ZDVHYDOXDWHGXVLQJ$%76)5$3DQG'33+
DVVD\V7RWDOSKHQROLFDQGÀDYRQRLGFRQWHQWZDVDOVRGHWHUPLQHG7KHKLJKHVWDQWLR[LGDWLYH
activity was detected with methanol extracts of A. coarctata '33+ ,& PJ
PL- )5$3 P0 J)H $%76 $$(& PJ PL ,& PJ PL)
and H. waldsteinii '33+ ,& PJ PL )5$3 P0 J)H$%76$$(&
PJmL,& PJPL7KHWRWDOSKHQROLFFRQWHQWYDULHGIURPȝJPL
WRȝJmLZKLOHWKHWRWDOÀDYRQRLGFRQFHQWUDWLRQVZHUHLQWKHUDQJHIURPȝJ
PLWRȝJ PL7KH WRWDO SKHQROLF FRQWHQW ZDV WKH KLJKHVW LQ WKH PHWKDQRO H[WUDFW
RI A. coarctata JPLZKLOHWKHWRWDOÀDYRQRLGFRQWHQWZDVLQWKHPHWKDQROH[WUDFW
RIH. waldsteinii J PL 2EWDLQHG UHVXOWV VKRZHG D FRUUHODWLRQ EHWZHHQ DQWLR[
LGDWLYH DFWLYLW\ DQGFRQFHQWUDWLRQVRIWKHSKHQROLFFRPSRXQGV
Key words: Achillea, Hieracium, leaf extracts, antioxidative activity

ANTIOXIDATIVE ACTIVITY OF LEAF EXTRACTS OF

THREE ARTEMISIA SPECIES FROM SE EUROPE
3HGMD-DQDüNRYLü10LODQ*DYULORYLü1-HOLFD1RYDNRYLü1, Nemanja
5DMþHYLü1'DQLMHOD6WHãHYLü2, Petar D. Marin1

2
8QLYHUVLW\RI0RQWHQHJUR)DFXOW\RI1DWXUDO6FLHQFHVDQG0DWKHPDWLFV'åRUGåD
9DãLQJWRQDEE3RGJRULFD0RQWHQHJUR
,Q WKH SUHVHQW VWXG\ DQWLR[LGDWLYH DFWLYLW\ RI PHWKDQROLF HWKDQROLF DQG HWK\ODFHWDWH
extracts of leaves from three Artemisia (Compositae) species (A. campestris / IURP
Montenegro, A. lobeli $OO YDU canescens '& %ULTX IURP 6HUELD DQG A. absinthium /
IURP5RPDQLDZHUHLQYHVWLJDWHG$OOWKUHHVSHFLHVDUHXVHGLQWUDGLWLRQDOPHGLFLQHDQGLWLV
GRFXPHQWHGWRSRVVHVVPHGLFLQDOSURSHUWLHV7KHH[WUDFWVZHUHREWDLQHGXVLQJWKHVWDQGDUG
SURFHGXUHZLWK0H2+(W2+DQG(W2$FVWRUHGLQGDUNDWURRPWHPSHUDWXUHIRUK
7KHDQWLR[LGDWLYHFDSDFLW\ZDVDVVHVVHGXVLQJ'33+$%76DQG)5$3DVVD\V7RWDOSKHQROLF
DQGÀDYRQRLGFRQWHQWZDVDOVRGHWHUPLQHG7KHKLJKHVWDQWLR[LGDWLYHDFWLYLW\LQ'33+DVVD\
ZDVGHWHFWHGZLWK0H2+DQG(W2+H[WUDFWVRIA. l.YDUcanescens,& PJ
PL DQG,& PJPLUHVSHFWLYHO\7KHKLJKHVWDQWLR[LGDWLYHDFWLYLW\LQ$%76DV
VD\ZDVGHWHFWHGZLWK0H2+H[WUDFWRIA. campestris$$(& PJPL,&
PJ PL, ZKLOH LQ )5$3 DVVD\ WKH KLJKHVW DQWLR[LGDWLYH DFWLYLW\ ZDV GHWHFWHG ZLWK
(W2+H[WUDFWof A. campestrisP0J)H$OOH[WUDFWVRIA. absinthiumVKRZHGZHDN
DQWLR[LGDWLYHDFWLYLW\ 7KH WRWDO SKHQROLF FRQWHQW YDULHG IURP ȝJ PL WR ȝJ
PL, while the WRWDOÀDYRQRLGDPRXQWZDVLQUDQJHIURPȝJPLWRȝJPL
7RWDO SKHQROLF DQG ÀDYRQRLG FRQWHQW ZDV WKH KLJKHVW LQ (W2+ H[WUDFWV RI A. l. YDU
canescens DQGJPLUHVSHFWLYHO\*LYHQUHVXOWVVKRZHGDFRUUHODWLRQEH
WZHHQDQWLR[LGDWLYHDFWLYLW\DQG FRQWHQW RI SKHQROLF FRPSRXQGV 7KHVH UHVXOWV LQGLFDWH WKDW
A. l. YDU canescens and A. campestris have the good antioxidative capacity and could be
considered as natural sources of DQWLR[LGDQWDJHQWV
Key words: Artemisia, leaf extracts, antioxidative activity

DETERMINATION OF TOTAL HYPERICINS OF

HYPERYCUM PERFORATUM L., FROM DIFFERENT
LOCATIONS IN WESTERN R. MACEDONIA
$ULMHWD6KDEDQL0DULMD.DUDSDQG]RYD,YDQD&YHWNRYLNM.DUDQ¿ORYD
Gjoshe Stefkov, Svetlana Kulevanova
,QVWLWXWHRI3KDUPDFRJQRV\)DFXOW\RI3KDUPDF\6V&\ULODQG0HWKRGLXV8QLYHUVLW\
6NRSMH50DFHGRQLD
Hypericum perforatum / +3 FRQWDLQV QXPHURXV FRPSRXQGV ZLWK GRFXPHQWHG ELRORJLFDO

DFWLYLW\ 7KH PRVW LQWHUHVWHG FRQVWLWXHQWV LQFOXGH QDSKWKRGLDQWKURQHV K\SHULFLQ DQG
pseudohypericin which have been used for the treatment of neurological disorders and
GHSUHVVLRQ,QDGGLWLRQK\SHULFLQKDVVKRZQDQWLYLUDODFWLYLW\DJDLQVWVHYHUDOW\SHVRIYLUXVHV
LQFOXGLQJWKHKXPDQLPPXQRGH¿FLHQF\YLUXV+,9DQGKDVEHHQWHVWHGDVDSKRWRVHQVLWL]HU
LQWKHWUHDWPHQWRIFDQFHU$FFRUGLQJWRWKLVIDFWWKHDLPRIWKLVVWXG\ZDVWRGHWHUPLQHWKH
OHYHORIWRWDOK\SHULFLQVH[SUHVVHGDVK\SHULFLQIURPDHULDOSDUWVRI+3FROOHFWHGIURPGLIIHUHQW
ORFDWLRQVLQ:HVWHUQ50DFHGRQLDGXULQJWKH\HDU+\SHULFLQFRQWHQWRIWKHVDPSOHV
ZDVPHDVXUHGDWQPXVLQJ899LVVSHFWURSKRWRPHWU\7KHDQDO\VLVVKRZHGWKDWOHYHOV
of hypericin varied depending on the location and the time of collection as well as the part
RIWKHSODQWKHUEDÀRZHURUOHDIWKDWKDVEHHQLQYHVWLJDWHG7KHORZHVWOHYHORIK\SHULFLQ
ZDVGHWHFWHGLQOHDYHVRI+3FROOHFWHGLQ0DYURYRZKLOHWKHKLJKHVW
ZDVGHWHFWHGLQ+3ÀRZHUVFROOHFWHGLQ7HWRYR,QFRQFOXVLRQWKHDHULDOSDUWVIURP
Macedonian wild- growing Hypericum perforatum can be considered as a good source of
K\SHULFLQVLQDFFRUGDQFHWRWKHUHTXLUHPHQWVRIWKH(XURSHDQ3KDUPDFRSRHLD
Key words: Hypericum perforatumK\SHULFLQVSHFWURSKRWRPHWU\

EVALUATION OF INHIBITORY POTENTIAL OF

LIGUSTICUM ALATUM (Bieb.) Sprengel ESSENTIAL OILS
AND EXTRACTS AGAINST ENZYMES INVOLVED IN
ALZHEIMER DISEASE AND TYPE II DIABETES
Gulmira Ozek1$QWRDQHWD7UHQGD¿ORYD2, Suleyman Yur1,3, Ceyda A.
Berdanoglu1, Fatih Goger1,3, Temel Ozek1,3

'HSDUWPHQWRI3KDUPDFRJQRV\)DFXOW\RI3KDUPDF\$QDGROX8QLYHUVLW\(VNLVHKLU
2
3
(VNLVHKLU7XUNH\
,Q WKHSUHVHQW ZRUN WKH HVVHQWLDO RLOV KH[DQH DQG PHWKDQROH[WUDFWVREWDLQHGIURPWKHOHDI

and fruits of Ligusticum alatum%LHE6SUHQJHOZHUHLQYHVWLJDWHGIRUFKHPLFDOFRPSRVLWLRQ
IUHH UDGLFDO '33+ and ABTS) scavenging potential and inhibitory effects against
acetylcholinesterase (from Electrophorus electricus) and porcine pancreatic _DP\ODVH
$FHW\OFKROLQHVWHUDVH LQKLELWLRQ RI WKH VDPSOHV ZDV HYDOXDWHG XVLQJ (OOPDQ¶V PHWKRG
Antidiabetic potentials of the extracts and the oils were evaluated via measurement of inhibition
of _DP\ODVHHQ]\PHXVLQJ.,,PHWKRG(SR[\WUDQVSVHXGRLVRHXJHQ\ODQJHODWH
(DQHWKROHFDU\RSK\OOHQHR[LGH`FDU\RSK\OOHQHDQGa-himachalene
ZHUHGHWHFWHGLQWKHOHDI,QWKHIUXLWV=ÌDUQHVHQH(DQHWKROH
HSR[\WUDQVSVHXGRLVRHXJHQ\ODQJHODWH]L]DQHQHaKLPDFKDOHQHDQG
_]LQJLEHUHQH ZHUH IRXQG DV PDMRU FRQVWLWXHQWV 7KH HVVHQWLDO RLOV DQG WKH H[WUDFWV
of L. alatumGHPRQVWUDWHGDQWLDFHW\OFKROLQHVWHUDVHDFWLYLW\UDQJHGEHWZHHQDQG
7KHKLJKHVW7($&YDOXHVZHUHREWDLQHGIRUWKHH[WUDFWREWDLQHGZLWKPHWKDQRO
P0DQGWKHHVVHQWLDORLOP0IURPWKHOHDI7KHH[WUDFWVGHPRQVWUDWHGZHDN
LQKLELWRU\HIIHFW,QKXSWRWRZDUGVWR_DP\ODVH7KHSUHVHQWZRUNLVWKH¿UVW
contribution about biological potential of Ligusticum alatumIUXLWDQGOHDIH[WUDFWV
Key words: Ligusticum alatum, composition, _-amylase, acetylcholinesterase, antioxidant
Acknowledgement: Authors thanks to TUBITAK (Project No 116S021) and BAS (Bilateral

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RAMAN SPECTROSCOPIC CHARACTERIZATION

OF CAROTENOIDS FROM ROSE HIPS HERBAL TEA
MIXTURES
Stefan Kolašinac1,OLQND3HüLQDU16WHYD/HYLü2'UDJDQD5DQþLü1, Zora
'DMLü6WHYDQRYLü1, Hartwig Schulz3

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University of Belgrade, Faculty of Agriculture, Department of Chemistry and Biochemistry,
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been recently considered as a complex of species (the aggregate) due to genetic and related
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beta-carotene, and rubixanthin, in addition to considerable content of vitamin C, the total
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different type of carotenoids in rose hip fresh fruit as well as in local Serbian herbal tea products
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FP$QDO\VLVRIWKHYLEUDWLRQPRGHDWFP strongly indicates the predominance of
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The preliminary results show that Raman spectroscopy can be used in carotenoid analysis in
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Key words: rose hip, herbal tea, spectroscopy method, beta-carotene

MICROMERIA CROATICA (Pers.) Schott - CHEMICAL

COMPOSITION AND VARIABILITY OF ESSENTIAL OIL
DEPENDING ON LOCALITY
Elma Vuko1, Gordana Rusak29DOHULMD'XQNLü1, Dario Kremer3, Ivan
Kosalec 3, Biljana Apostolska1DQG1DGD%H]Lü1

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Split, Croatia
2
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Zagreb, Croatia
3
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Croatia
7KHHVVHQWLDORLO(2ZDVLVRODWHGIURPMicromeria croatica3HUV6FKRWWDQHQGHPLFSODQW

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detailed GC and GC-MS analysis to determine chemical composition and the similarity and
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studied populations of M. croaticaFDQEHFRQVLGHUHGWREHTXLWHXQLIRUP$VRPHZKDWPRUH
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Key words: Micromeria croaticaHVVHQWLDORLOȕFDU\RSK\OOHQHFDU\RSK\OOHQHR[LGH

INFLUENCE OF RHODIOLA ROSEA L. CALLI

EXTRACTS ON CD4 T CELLS PHENOTYPE CHANGES
Andrey S. Marchev1, Petya A. Dimitrova2, Kalin Stoyanov3, Milen I.
Georgiev1

2
Department of Immunology, The Stephan Angeloff Institute of Microbiology, Bulgarian
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Bulgaria
Rhodiola rosea/H[WUDFWDQGLWVPDMRUELRDFWLYHFRQVWLWXHQWVVDOLGURVLGHURVDULQURVDYLQDQG

rosin) have been found to alter CD3 T cell growth and apoptosis via modulation of IFN-gamma
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roseaFDOOLH[WUDFWVDQGSW\URVRODWORZGRVHPD\KDYHSRWHQWLDOWRDIIHFWWKHSKHQRW\SHSUR¿OH
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Key words: Rhodiola rosea, calli, T cells, Foxp3, immunity
Acknowledgement: This study was supported by Program for career development of young
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HPLC/DAD/ESI-MSN ANALYSIS OF PHENOLIC

COMPOUNDS IN HYPERICUM PERFORATUM L. WILD-
GROWING PLANTS FROM PELISTER NATIONAL
PARK, REPUBLIC OF MACEDONIA
Oliver Tusevski1, Marija Krstikj1, Jasmina Petreska Stanoeva2, Marina
Stefova2, Sonja Gadzovska Simic1

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2
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The aim of this study was to provide comprehensive understanding of phenolic compounds
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SKHQROLF DFLGV TXLQLF DFLG ZDV GHWHFWHG LQ DOO WHVWHG H[WUDFWV ZKLOH FKORURJHQLF DFLG DQG
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DFLGZDVGHWHFWHGLQ52DQG)6EXWQRWLQ1)6H[WUDFWV5HJDUGLQJWKHÀDYDQROVHSL
catechin and several oligomeric procyanidins were found in all tested samples, whereas
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2UKDPQRVLGH ZDV FRQ¿UPHG LQ 1)6 DQG )6 1DSKWKRGLDQWKURQHV ZHUH UHSUHVHQWHG
with pseudohypericin, hypericin and protopseudohypericin in FS, while only hypericin
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GHSUHQ\OUKHHGLD[DQWKRQH%JDUFLQRQH&GLK\GUR[\WULPHWKR[\[DQWKRQHFDGHQVLQ
* FDGHQVLQ & DQG VHYHUDO [DQWKRQH GHULYDWLYHV ZDV H[FOXVLYHO\ FRQ¿UPHG RQO\ LQ 52
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[DQWKRQHV LQ 1)6 )6 DQG 52 IURP H. perforatum that can be used as biologically active
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Key words:+3/&'$'(6,06QHypericum perforatum/SKHQROLFFRPSRXQGV

PRODUCTION OF NITRIC OXIDE, REACTIVE OXYGEN

SPECIES AND COX2 BY NEUTROPHILS UNDER THE
INFLUENCE OF CLINOPODIUM VULGARE L.
Kristiana Amirova1, Petya Dimitrova2, Kalin Stoyanov3, Deyan
Donchev4, Ivanka Koycheva1,5, Andrey S. Marchev1,5, Milen I.
Georgiev1,5

Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria
2
Department of Immunology, The Stephan Angeloff Institute of Microbiology, Bulgarian
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Bulgaria

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content of chlorogenic and caffeic acids, as well as, catechin of C. vulgare from Pirin
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gUHVSHFWLYHO\7KHLQÀXHQFHRIPHWKDQROH[WUDFWRIC. vulgare has been investigated
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ORZ GRVHV J P/) the plant extract reduced twice the apoptosis in both neutrophil
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VKRFNQHXWURSKLOSRSXODWLRQV7KHREWDLQHGGDWDVKRZHGWKDWWKHH[WUDFWRIC. vulgare may
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Key words: Clinopodium vulgare/QHXWURSKLOVLQÀDPPDWLRQSKHQROLFVR[LGDWLYHVWUHVV

free radicals

Acknowledgement: This project for establishment of CPSBB has received funding from The
European Union’s Horizon 2020 research and innovation programme under grant agreement
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CHEMICAL COMPOSITION AND FREE RADICAL

SCAVENGING PROPERTIES OF PULICARIA
SALVIIFOLIA Bunge., P. GNAPHALODES (Vent.) Boiss.
AND P. ULIGINOSA Steven ex DC. ESSENTIAL OILS
Komila Eshbakova1, Gulmira Ozek26OH\PDQ<XU2,3, Temel Ozek2,3

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2
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,Q WKH SUHVHQW ZRUN WKUHH Pulicaria VSHFLHV FROOHFWHG LQ 8]EHNLVWDQ Pulicaria salviifolia
%XQJH P. gnaphalodes 9HQW %RLVV DQG P. uliginosa 6WHYHQ H[ '& ZHUH VXEMHFWHG WR
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aWHUSLQHQH ZPXXURORO DQG aFDGLQHQH ZHUH IRXQG DV WKH PDLQ
FRQVWLWXHQWV7KHRLORIP. gnaphalodes was found to be rich with oxygenated monoterpenes,
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FKU\VDQWKHQRQH DV ZHOO DV R[\JHQDWHG VHVTXLWHUSHQHV FDGLQDGLHQ_-ol
DQGHXGHVPDGLHQ`RO7KHRLORIP. uliginosa was found to contain
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HQRQH 7FDGLQRO bFDGLQHQH FDU\RSK\OOHQH R[LGH
JHUPDFUDGLHQ`RODQGSXOHJRQHDVWKHPDLQFRQVWLWXHQWV7KHRLOV
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PulicariaVSHFLHV
Key words: Pulicaria, essential oil, gas chromatography, antioxidant

COMPARATIVE ANALYSIS OF VOLATILE OILS

OBTAINED FROM SEVEN ROMANIAN BRANDS OF
CHAMOMILE TEA
Marin Zagnat, Corina Cheptea
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&KDPRPLOH WHD LV RQH RI WKH PRVW SRSXODU WHDV LQ 5RPDQLD IRU LWV WKHUDSHXWLF TXDOLWLHV DV
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,WLVDOVRUHVSRQVLEOHIRUWKHWDVWHDQGHVSHFLDOO\IRUWKHVPHOORIWKHYHJHWDEOHSURGXFW7KDW¶V
ZK\ZHKDYHGHFLGHGWRREWDLQDQGDQDO\VHWKHYRODWLOHRLOVRIWKHSXUFKDVHGSURGXFWV9RODWLOH
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RSWLPL]HGIRUWKHDQDO\VHGSURGXFW7KHVDPSOHVL]HRISODQWSURGXFWDQGWKHDPRXQWRIKH[DQH
were according to the European Pharmacopoeia 5th(G7KHREWDLQHGVROXWLRQVRIFKDPRPLOH
RLOLQKH[DQHZHUHRIYDULRXVVKDGHVRIEOXHIURPSDOHWRLQWHQVH7KHUHVXOWVRIWKHDQDO\VHV
VKRZHG FRQVLGHUDEOH GLIIHUHQFHV EHWZHHQ WKH REWDLQHG YRODWLOH RLOV ERWK TXDOLWDWLYHO\ DQG
HVSHFLDOO\TXDQWLWDWLYHO\9RODWLOHRLOFRQFHQWUDWLRQVLQWKHSXUFKDVHGSURGXFWVYDULHGEHWZHHQ
DQG7KHREWDLQHGUHVXOWVVKRZRQFHDJDLQWKHQHFHVVLW\WRDSSO\PRUHVWULQJHQW
UHTXLUHPHQWVIRUPHGLFLQDOSODQWVDQGIRUKHUEDOSURGXFWV
Key words: chamomile, volatile oil, microwave-assisted hydrodistillation,

PHENOLIC COMPOUNDS IN SELECTED PLANT

SPECIES
Helena Pluhackova1, Rastislav Boško1, Sylvie Belakova2

Department of Crop Science, Breeding and Plant Medicine, Mendel University in Brno,
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2
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high antioxidant activity useful for the prevention of cardiovascular diseases, bacterial growth
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DWWKH5HVHDUFK,QVWLWXWHRI%UHZLQJDQG0DOWLQJ3/&7KHREWDLQHGUHVXOWVLQGLFDWHWKDWWKH
extraction of free phenolic compounds seems to be the best method for obtaining phenolic
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GLYCINE MAX L. Merr. SECONDARY METABOLITES AS

NUTRACEUTICALS
Georgina Kosturkova1, Milena Nikolova2, Margarita Dimitrova1,
Rositsa Todorova3, Sashank Tidke4, Ravishankar Gokare4

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Bulgaria
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Dayananda Sagar Institutes, Bangalore, India
Glycine max/0HUULVZRUOGIDPRXVIRULWVKLJKFRQWHQWRISK\WRSURWHLQVRLOV

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Bulgarian Glycine maxYDULHWLHVIRUWKHLUSRWHQWLDOWREHXVHGLQKHDOWKFDUHSURJUDPV
Key words: Glycine max, nutraceuticals, secondary metabolites
Acknowledgement: Presented investigations are under the Indo-Bulgarian Intergovernmental

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GLYCYRRHIZIN CONTENT IN THE ROOTS OF

GLYCYRRHIZA GLABRA L. FROM BULGARIAN
POPULATIONS
Asya Kozhuharova, Strahil Berkov, Marina Stanilova
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roots (Radix Glycyrrhizae) used for treatment of asthma, renal calculus, ulcer, psoriasis, and
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Thus, the licorice population near Beltsov was selected as the most appropriate source of
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ANTIOXIDANT CAPACITY AND TOTAL POLYPHENOL

CONTENT OF LAVENDER CULTIVARS (L.
ANGUSTIFOLIA Mill., L. X INTERMEDIA (L.) Emeric) AT
DIFFERENT GROWING AREAS IN HUNGARY
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Lavandula species are worldwide grown essential oil producing medicinal plants with
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were aimed at studying the antioxidant capacity and total polyphenol content of samples
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VARIABILITY OF TOTAL ESSENTIAL OILS CONTENT

IN LEAVES OF BAY LAUREL (LAURUS NOBILIS L.) IN
12 DISTINCT POPULATIONS OF CROATIAN ADRIATIC
AREA IN RELATION WITH GEOGRAPHIC POSITION
OF LOCATIONS, LEAF LAMINA SURFACE AND
GENETIC VARIABILITY
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variability, and variability of total essential oils content in leaves of bay laurel populations,
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results, it is possible to conclude that the populations of bay laurel from locations of South East
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Key words: bay laurel, Laurus nobilis //DXUDFHDHHVVHQWLDORLOV

ANTIDEPRESSANT-LIKE EFFECT OF SALIDROSIDE

AND CURCUMIN ON THE IMMUNOREACTIVITY OF
ANIMALS SUBJECTED TO A CHRONIC MILD STRESS
MODEL
Liliya Vasileva1,2,6, Kremena Saracheva2, Mariya Ivanovska3,4, Atanaska
Petrova3, Andrey Marchev1,5, Milen Georgiev1,5,Mariana Murdjeva3,4,
Damyanka Getova6

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Technological Center for Emergency Medicine (TCEMED), Division of Immunological
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by combination of salidroside and curcumin suggesting synergic interaction regarding their
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II. DO PLANT GROWTH REGULATORS INFLUENCE

HYSSOP (HYSSOPUS OFFICINALIS L.) ANTIOXIDANT
SYSTEM DURING MICROPROPAGATION?
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Micropropagation of +\VVRSXVRI¿FLQDOLV/RIIHUVWKHEHVWWHFKQLTXHIRUTXDOLW\DQGGLVHDVH
free uniform plant material, but there are no studies have been conducted on the effect of plant
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and guaiacol peroxidase activities was measured when plants are cultured on the MS medium
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of water- and lipid-soluble metabolites with antioxidant capacity expressed as ascorbate or
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levels were observed in the MS medium without growth regulators, followed by the treatments
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of low molecular metabolites – water and lipid soluble metabolites with antioxidant capacity,
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metabolites
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VACCINIUM MACROCARPON Aiton (CRANBERRY)

EXTRACT CAUSES THE RELAXATION IN RAT CORPUS
CAVERNOSUM TISSUE
Alev Önder1, Serap Gur1, Didem Yilmaz-Oral1,2

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Vaccinium macrocarpon Aiton (Ericaceae), commonly called cranberry, is native to Eastern

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SDWKZD\ 2YHUDOO WKHVH in vitro studies suggested that consumption of cranberry-E may be
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of the current study could be used by physicians to recommend cranberry extract ingestion to
GHFUHDVHWKHLQFLGHQFHRI('SDWLHQWVZLWK87,DVDQDOWHUQDWLYHWKHUDS\
Key words: cranberry, ED, Vaccinium, Ericaceae

BLOCK COPOLYMER NANOCARRIERS OF CAFFEIC

ACID PHENETHYL ESTER: PREPARATION AND
ANTIOXIDANT ACTIVITY
V. Bankova1, M. Popova1, B. Trusheva1, G. Grancharov2, Y. Yordanov3,
D. Aluani3, V. Tzankova3, M. Kondeva-Burdina3, B. Tzankov3, K.
Yoncheva3, P. Petrov2

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2
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3
0HGLFDO8QLYHUVLW\RI6R¿D'XQDYVW6R¿D%XOJDULD
Caffeic acid phenethyl ester (CAPE) is one of the most important constituents of poplar
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WKH \LHOGV UHDFWLRQ WLPHV DQG WKH UDWLR (= LVRPHUV RI WKH REWDLQHG SURGXFW 1H[W &$3(
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PHGLXP DQG HQKDQFHG ELRDYDLODELOLW\ 6SKHULFDO QDQRVL]HG PLFHOOHV ZHUH SUHSDUHG IURP D
SRO\HWK\OHQHR[LGHESRO\¡FDSURODFWRQESRO\HWK\OHQHR[LGH3(2E3&/
3(2 WULEORFN SRO\PHU V\QWKHVL]HG E\ ³FOLFN´ UHDFWLRQ 7KH PLFHOODU V\VWHP FRPSULVLQJ D
ELRGHJUDGDEOH3&/FRUHFRQWDLQLQJ&$3(DQGDK\GUDWHG3(2VKHOOH[KLELWHGVXSHULRUFROORLG
VWDELOLW\LQDTXHRXVPHGLD7KHQWKHDQWLR[LGDQWDFWLYLW\RI&$3(ORDGHGPLFHOOHVRQDPRGHO
RIK\GURJHQSHUR[LGH±GDPDJHG+HS*FHOOVZDVVWXGLHGDQGFRPSDUHGWRWKDWRISXUH&$3(
&HOOV ZHUH SUHWUHDWHG ZLWK WKH VXEVWDQFHV LQ WKH FRQFHQWUDWLRQ LQWHUYDO ± J PL
and WKHQ GDPDJHG ZLWK ȝ0 +222 6LJQL¿FDQW SURWHFWLYH HIIHFWV RI ERWK ORDGHG DQG
QRQORDGHG &$3( ZHUH REVHUYHG DW FRQFHQWUDWLRQ J PL, revealing that the micellar
carriers UHWDLQWKHDQWLR[LGDQWSURSHUWLHVRI&$3(
Key words:FDIIHLFDFLGSKHQHWK\OHVWHUEORFNFRSRO\PHUQDQRFDUULHUVDQWLR[LGDQWDFWLYLW\
Acknowledgement: The work was supported by the National Science Fund of Bulgaria
(DN-09/1-2016).

PROPOLIS FROM VIETNAM – CHEMICAL PROFILES,

PLANT ORIGIN AND BIOLOGICAL ACTIVITY
M. Popova1, B. Trusheva1, V. Bankova1, K. Georgieva1, Le Nguyen
Thanh2, I. Tsvetkova3, H. Najdenski3

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6FLHQFHV$FDG*%RQFKHYVWUEO6R¿D%XOJDULD
2
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Vietnam
3
7KH6WHSKDQ$QJHORII,QVWLWXWHRI0LFURELRORJ\%XOJDULDQ$FDGHP\RI6FLHQFHV$FDG*
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3URSROLVLVDEHHKLYHSURGXFWLPSRUWDQWWRVRFLDOLPPXQLW\RIWKHEHHFRORQ\,WLVZHOONQRZQ
to humans for its preventive and healing properties and nowadays it is included in numerous
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VLWHRIUHVLQFROOHFWLRQ7KHVWXG\RISURSROLVIURPXQH[SORUHGVFDQWLO\H[SORUHGUHJLRQVKDV
the potential to uncover new biologically active compounds with important pharmacological
HIIHFWV:HVWXGLHGSURSROLVIURP9LHWQDPWZRVDPSOHVRIApis mellifera propolis and four
VDPSOHVIURPORFDOVWLQJOHVVEHHV&KHPLFDOSUR¿OHVRIWKHVDPSOHVZHUHREWDLQHGE\*&06
DQDO\VLVRIWKHLUHWKDQROH[WUDFWV7KHKRQH\EHHVDPSOHVZHUHFKDUDFWHUL]HGE\WKHSUHVHQFH
RIDON\OSKHQROVDON\OSKORURJOXFLQROVDQDFDUGLFDFLGVDQGWULWHSHQRLGVW\SLFDOFRQVWLWXHQWV
of Mangifera indica / D SODQW ZHOONQRZQ DV SURSROLV VRXUFH LQ WURSLFDO UHJLRQV 2Q WKH
other hand, the stingless bee propolis samples differed between them and from the A. mellifera
samples, but all of them had varying amounts of the typical metabolites of the resin of the
Vietnamese tree Cratoxylum cochinchinense/RXU%OXPHVSHFL¿FSUHQ\ODWHG[DQWKRQHV7KH
samples with highest content of prenylxanthones demonstrated the highest radical scavenging
DFWLYLW\ DJDLQVW '33+7KH DQWLPLFURELDO DFWLYLW\ RI WKH VDPSOHV ZDV DOVR GHWHUPLQHG7KH
GHWDLOHGVWXG\RIWKHVWLQJOHVVEHHVSURSROLVLVLQSURJUHVV
Key words: propolis, plant source, biological activity

CHEMICAL CHARACTERIZATION AND RADICAL-

SCAVENGING ACTIVITY OF PROPOLIS FROM
PITCAIRN ISLAND
Boryana Trusheva, Kristina Georgieva, Rosa Dimitrova, Milena
Popova, Vassya Bankova
6FLHQFHV$FDG*%RQFKHY6WUEO6R¿D%XOJDULD
Propolis (bee glue) is a resinous hive product collected by bees from certain plant sources and
NQRZQVLQFHDQFLHQWWLPHVIRULWVKHDOLQJSURSHUWLHV7KHUHLVDQXPEHURIVFLHQWL¿FHYLGHQFH
WKDW LW KDV DQWLPLFURELDO DQWLR[LGDQW DQWLLQÀDPPDWRU\ LPPXQRPRGXODWLQJ DQWLXOFHU
DQWLGLDEHWLF DQG DQWLFDQFHU DFWLYLWLHV %HFDXVH RI LWV EURDG VSHFWUXP RI ELRORJLFDO DFWLYLWLHV
there is an undying interest on the composition of propolis, which depends on the vegetation of
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RI SURSROLV IURP (XURSH 6RXWK$PHULFD$VLD DQG WKH 3DFL¿F UHJLRQ H[LVW LQ WKH OLWHUDWXUH
KRZHYHUWKHUHLVQRGDWDDERXWSURSROLVIURP3LWFDLUQ,VODQGV,QWKHODVW\HDUVLWLVRIJURZLQJ
FRPPHUFLDOLQWHUHVWEHFDXVH3LWFDLUQ¶VEHHSRSXODWLRQLVGLVHDVHIUHHDQGWKHLUSURGXFWVDUH
FRQVLGHUHGDVFOHDQRISROOXWDQWV7KHDLPRIWKLVZRUNZDVWRGHWHUPLQHYRODWLOHDQGQRQ
volatile composition as well as radical-scavenging activity of propolis from Pitcairn Island
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K\GURGLVWLOODWLRQ H[WUDFWLRQ RI UDZ SURSROLV LQ D /LNHQV1LFNHUVRQ W\SH DSSDUDWXV DQG WKHQ
DQDO\]HGE\*&067KHPDLQYRODWLOHFRPSRQHQWVZHUHPRQRWHUSHQHVSPHQWKDGLHQ
RODQG_SLQHQHDQGVHVTXLWHUSHQHV`FDU\RSK\OOHQHFDU\RSK\OOHQH
R[LGHDQGYHUEHQRQH7KHPDMRUQRQYRODWLOHFRQVWLWXHQWVDFFRUGLQJWRWKH*&
06DQDO\VLVDIWHUVLO\ODWLRQZHUHWHUSHQRLGVPDLQO\GLWHUSHQHVDQGOHVVWULWHUSHQHV3KHQROLF
FRPSRQHQWVFDUGDQROVDONHQ\OUHVRUFLQROVDQGDQDFDUGLFDFLGVDUHSUHVHQWLQVLJQL¿FDQWO\
ORZHUDPRXQWVDVÀDYRQRLGVDQGWKHXVXDOO\SUHVHQWSKHQROLFDFLGVDUHFRPSOHWHO\DEVHQW2Q
WKHEDVLVRIWKHRYHUDOOFKHPLFDOSUR¿OHK\SRWKHVHVZHUHPDGHUHJDUGLQJWKHSODQWVRXUFHVRI
WKHVDPSOH'33+UDGLFDOVFDYHQJLQJDFWLYLW\RISURSROLVHWKDQROH[WUDFWZDVWHVWHGDQGWKH
UHVXOWVVKRZHGZHHNDFWLYLW\
Key words: propolis, GC/MS, volatiles, diterpenes, DPPH
Acknowledgement: The authors would like to thank to Pitcairn Island Producers’ Co-operative
(PIPCO) for providing the sample.

GC/MS BASED METABOLITE PROFILING OF

BULGARIAN GLYCINE MAX (L.) Merr.VARIETIES
Milena Nikolova1, Strahil Berkov1, Margarita Dimitrova2, Rositsa
Todorova3, Georgina Kosturkova2

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2
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Bulgaria
3
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Soybean (Glycine max / 0HUU LV RQH RI WKH PRVW ZLGHO\ FXOWLYDWHG FURSV LQ WKH ZRUOG
IRULWVQXWULWLRQDODQGPHGLFLQDOSURSHUWLHV,Q%XOJDULDVHULHVRIHOLWHVR\EHDQYDULHWLHVZLWK
LPSURYHG\LHOGDQGQXWULWLRQTXDOLWLHVKDVEHHQUHOHDVHGDWWKH Experimental Station in Soya,
3DYOLNHQL ,Q WKH SUHVHQW VWXG\ %XOJDULDQ VR\EHDQ VHHG FXOWLYDUV ZHUH H[DPLQHG DQG ZHUH
FRPSDUHGZLWK$PHULFDQYDULHW\³+RGVRQ´ZRUOGVWDQGDUGIRUWKHLUPHWDEROLWHSUR¿OHVE\
*&06$YDULHW\RIIDWW\DFLGVVDWXUDWHGDQGXQVDWXUDWHGDPLQRDFLGVLQGLVSHQVDEOHDQG
LQWHUFKDQJHDEOHRUJDQLFDQGSKHQROLFDFLGVDYDULHW\RIFDUERK\GUDWHVZDVIRXQG7KHVWXGLHG
Bulgarian cultivars showed comparable even in terms of extractable lipid components better
SUR¿OHV WKDQ ZRUOG VWDQGDUG ³+RGVRQ´7KH SUHVHQW UHVXOWV VKRZHG WKDW %XOJDULDQ VR\EHDQ
FXOWLYDUV DUH SURPLVLQJ IRRG ZLWK D KLJK QXWULWLRQDO YDOXH DQG EHQH¿FLDO WR KXPDQ KHDOWK
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Key words: Bulgarian cultivars, fatty acids, Glycine max
Acknowledgement: Presented investigations are under the Indo-Bulgarian Intergovernmental

3URJUDPIRU5 7&ROODERUDWLRQSURMHFW'176

CHEMOSYSTEMATIC SIGNIFICANCE OF
UNDERGROUND PARTS AND FRUIT ESSENTIAL OIL
CONSTITUENTS OF NINE HERACLEUM TAXA FROM
SOUTHEASTERN EUROPE
Ljuboš Ušjak16LOYDQD3HWURYLü1, Milica Drobac10DUMDQ1LNHWLü2

University of Belgrade - Faculty of Pharmacy, Department of Pharmacognosy, Vojvode
6WHSH%HOJUDGH6HUELD
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Chemical composition data of essential oils from underground parts and fruits of nine Heracleum
/WD[D$SLDFHDHFROOHFWHGLQ6HUELD0RQWHQHJUR0DFHGRQLDDQG6ORYHQLDZHUHVWDWLVWLFDOO\
DQDO\]HG WR HYDOXDWH FKHPRV\VWHPDWLF VLJQL¿FDQFH RI WKHLU FRPSRQHQWV (LJKW LQYHVWLJDWHG
taxa (H. orphanidis%RLVVDQGUHSUHVHQWDWLYHVRIH. sphondylium group: H. sphondylium/
H. sibiricum/H. montanum6FKOHLFKH[*DXGLQH. ternatum9HOHQH. pyrenaicum VXEVS
pollinianum%HUWRO)3HGURWWL 3LJQDWWLH. pyrenaicumVXEVSorsinii *XVV)3HGURWWL
3LJQDWWLDQGH. verticillatum3DQþLüEHORQJWRWKHW\SHVHFWLRQRIWKHJHQXV$GGLWLRQDOO\
DQDO\]HGWD[RQH. austriacumVXEVSsiifolium6FRS1\PDQEHORQJVWR+VHFW:HQGWLD
+RIIP'&(VVHQWLDORLOVZHUHK\GURGLVWLOOHGXVLQJ&OHYHQJHUW\SHDSSDUDWXVDQGDQDO\]HG
E\*&),'DQG*&06&KHPRV\VWHPDWLFVLJQL¿FDQFHRIFRQVWLWXHQWVZDVHYDOXDWHGXVLQJ
PXOWLYDULDWHVWDWLVWLFV3&$Q0'6DQG83*0$6WDWLVWLFDODQDO\VLVLQFOXGHGRXUSUHYLRXVO\
SXEOLVKHG GDWD RQ WKH RLOV RI VDPSOHV RI XQGHUJURXQG SDUWV DQG RI IUXLWV DV ZHOO DV
DGGLWLRQDOO\DQDO\]HGRLOVRIVDPSOHVRIXQGHUJURXQGSDUWVDQGRIIUXLWV8QGHUJURXQGSDUWV
oils of representatives of H. sphondylium JURXS ZHUH FKDUDFWHUL]HG E\ PRQRWHUSHQHV ZLWK
ȕSLQHQH=ȕRFLPHQHRUOLPRQHQHEHLQJGRPLQDQWDPRQJWKHPZKLOHH. orphanidis and
H. austriacumXQGHUJURXQGSDUWVRLOVZHUHGRPLQDWHGE\=IDOFDULQRO)UXLWRLOVZHUHULFKLQ
DOLSKDWLFHVWHUVVXFKDVRFW\ODFHWDWHWKHPHPEHUVRI+VHFW+HUDFOHXPDQGRFW\OKH[DQRDWH
(H. austriacum6WDWLVWLFDODQDO\VLVRIXQGHUJURXQGSDUWVHVVHQWLDORLOVVKRZHGWKDWLQYHVWLJDWHG
WD[DZHUHJURXSHGDFFRUGLQJWRWKHLUWD[RQRPLFFODVVL¿FDWLRQ6XEVSHFLHVRIH. pyrenaicum
were grouped together, and the members of H. sphondylium group were well separated from H.
orphanidis and H. austriacum5HJDUGLQJIUXLWHVVHQWLDORLOVVXFKUHODWLRQVKLSVZHUHFOHDUO\
REVHUYHGLQWKUHHGLPHQVLRQDOQ0'63&$UHYHDOHGWKDWGLIIHUHQWFRPSRXQGVQRWRQO\DERYH
PHQWLRQHGGRPLQDQWRQHVLQÀXHQFHGWKHVHSDUDWLRQRILQYHVWLJDWHGWD[D
Key words: Heracleum underground parts and fruits, essential oils, GC-FID and GC-MS,
multivariate statistics, chemosystematics

SCREENING OF CYTOTOXICITY OF CENTAUREA

SPECIES AQUEOUS EXTRACTS FRACTIONS ON
HUMAN FIBROBLASTS
Anja Golemac1, Sanja Radman1%RåHQD6RþLN1, Francois Xavier
Pellay1, Ivana Carev2, Olivera Politeo2

0HGLWHUUDQHDQ,QVWLWXWHIRU/LIH6FLHQFHV6SOLW&URDWLD
2
Faculty of Chemistry and Technology, Split, Croatia
CentaureaVSHFLHVDUHXVHGDVPHGLFDOSODQWVRUDVDIRRGLQHDVWHUQ0HGLWHUUDQHDQDUHD7KH
DLPRIRXUVWXG\ZDVWRVFUHHQWKHF\WRWR[LFLW\RIWZHOYHGLIIHUHQWDTXHRXVH[WUDFWVIURPVHYHQ
Croatian CentaureaVSHFLHVDQGWRFRPSDUHWKHLU+3/&SUR¿OHWRLGHQWLI\WR[LFIUDFWLRQV7KH
toxic properties of plant extracts and their constituents may be used in pharmaceutical and
FRVPHWLFLQGXVWU\&\WRWR[LFLW\RICentaurea extracts and fractions was measured on primary
¿EUREODVWVWKURXJKWKH077DVVD\7KHH[WUDFWVZHUHSDUWLWLRQHGXVLQJUHYHUVHGSKDVH+3/&
ZLWK&FROXPQDQGJUDGLHQWRISRODUVROYHQWV$TXHRXVH[WUDFWV$(ZHUHSUHSDUHGIURP
wild growing Centaurea ragusina /C. scabiosa /C. solstitialis /C. rupestris /C. alba
/., C. jacea/DQGC. salonitana 9LV$PRQJWKHWHVWHGDTXHRXVH[WUDFWVWKHKLJKHVWF\WRWR[LF
activity was found in C. ragusina, C. salonitana, C. solstitialis and C. alba(YHU\RWKHU$(
showed low toxicity and C. scabiosa, wild edible VSHFLHVVKRZHGQRWR[LFLW\HYHQDWJ/RI
H[WUDFW6RPHRIWKHIUDFWLRQVLVRODWHGE\+3/&ZHUHWHVWHGIRUWKHLUWR[LFLW\EDVHGRQWKHLU
+3/&SUR¿OH3HDNVWKDWZHUHFKDUDFWHULVWLFRIHDFKVSHFLHVZHUHFROOHFWHGDQGWHVWHGE\077
RQ¿EUREODVWDWWKHHTXLYDOHQWIRUJ/RIH[WUDFW6RPHRIWKHVHIUDFWLRQVVHHPWRH[SODLQSDUW
RIWKHWR[LFLW\RIWKHH[WUDFW)XWXUHLQYHVWLJDWLRQVRIFKHPLFDOFRPSRVLWLRQVKRXOGKLJKOLJKW
WKH PDLQ FRPSRXQGV LQ WKHVH H[WUDFWV UHVSRQVLEOH IRU WKRVH ELRORJLFDO DFWLYLWLHV$V IDU DV
previous research showed, it is expected that some of the main groups of compounds could
EHVHVTXLWHUSHQHODFWRQHVSKHQROLFFRPSRXQGVDQGRUJDQLFDFLGV6HVTXLWHUSHQHODFWRQHVDUH
usually connected with toxic properties of Centaurea extracts
Key words: Centaurea, extracts, cytotoxicity

GC/MS BASED METABOLITE PROFILING AND

ANTIOXIDANT ACTIVITY OF BALKAN AND
BULGARIAN ENDEMIC PLANTS
Milena Nikolova1, Ina Aneva I1, Petar Zhelev2, Strahil Berkov1

Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences,
E$FDG*%RQFKHY6R¿D%XOJDULD
2
8QLYHUVLW\RI)RUHVWU\.OLPHQW2KULGVN\6R¿D%XOJDULD
%DONDQDQG%XOJDULDQHQGHPLFSODQWVViola rhodopaea%HFNHU9LRODFHDHVeronica rhodopaea

9HOHQ 'HJHQ H[ 6WRM 6WHIDQRY 3ODQWDJLQDFHDH Silene roemeri )ULY &DU\RSK\OODFHDH
Jasione bulgarica6WRM 6WHIDQGCampanula lanata)ULY&DPSDQXODFHDHZHUHH[DPLQHG
0HWDEROLWHSUR¿OHVRIPHWKDQROLFH[WUDFWVRIVWXGLHGVSHFLHVZHUHDQDO\VHGE\*&06DQG
+37/&$QWLR[LGDQW SRWHQWLDO ZDV DVVD\HG E\ '33+ PHWKRG DQG ȕFDURWHQHOLQROHLF DFLG
WHVW7KHKLJKHVWDQWLR[LGDQWSRWHQWLDOZDVHVWLPDWHGIRUWKHH[WUDFWVRIVeronica rhodopaea,
Jasione bulgarica and Viola rhodopaea)DWW\DFLGVDQGDOFRKROVSKHQROLFDQGRUJDQLFDFLGV
ÀDYRQRLGVVWHUROVFDUERK\GUDWHVZHUHLGHQWL¿HG7KHSUHVHQWGDWDDUHUHSRUWHGIRUWKH¿UVW
WLPHIRUWKHVWXGLHGVSHFLHV
Key words: Viola rhodopaea, Jasione bulgarica, Campanula lanata, Veronica rhodopaea,
Silene roemeri

PULMONARIOSIDES A AND B – NEW PHENOLIC

COMPOUNDS FROM THE AERIAL PART OF
PULMONARIA OFFICINALIS L.
-XVW\QD.U]\ĪDQRZVND.RZDOF]\NàXNDV]3HFLR0DULXV].RZDOF]\N
Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research
,QVWLWXWHXO&]DUWRU\VNLFK3XáDZ\3RODQG
3XOPRQDULDRI¿FLQDOLV/OXQJZRUWEHORQJLQJWR%RUDJLQDFHDHIDPLO\LVFRQVLGHUHGWRSRVVHVV

WKHUDSHXWLF SURSHUWLHV ,W KDV D ORQJ WUDGLWLRQ RI XVH LQ IRON PHGLFLQH DV D UHPHG\ DJDLQVW
YDULRXV OXQJ GLVRUGHUV LQFOXGLQJ WXEHUFXORVLV DVWKPD DQG FRXJKV 7KLV KHUE DOVR KDV WKH
H[SHFWRUDQWDQWLLQÀDPPDWRU\DQWLWXVVLYHDQGGLDSKRUHWLFSURSHUWLHV8VHGH[WHUQDOO\LWFDQ
EHYHU\EHQH¿FLDOLQWKHWUHDWPHQWRIEXUQVZRXQGVFXWVDQGHF]HPD+RZHYHUYHU\IHZ
SKDUPDFRORJLFDOVWXGLHVFRQ¿UPLQJWKHHIIHFWVRIWKHWUDGLWLRQDOXVHRIPulmonariae Herba
DUH DYDLODEOH 6LPLODUO\ VFLHQWL¿F OLWHUDWXUH GHVFULELQJ VHFRQGDU\ PHWDEROLWHV UHVSRQVLEOH
IRU WKH ELRORJLFDO DFWLYLW\ RI WKLV KHUE LV YHU\ VSDUVH DQG VHOHFWLYH$OWKRXJK D IHZ VWXGLHV
concerning phenolic compounds are available, other classes of compounds are practically
XQNQRZQ :H HPSOR\HG PXOWLVWDJH LVRODWLRQ SURFHGXUH LQFOXGLQJ VROLG SKDVH H[WUDFWLRQ
DQG IHZ FKURPDWRJUDSKLF VWHSV WR IXUWKHU FKDUDFWHUL]H Pulmonariae Herba and to isolate
QHZ FRPSRXQGV7KHLU VWUXFWXUHV ZHUH GHWHUPLQHG EDVHG RQ LWV +5472)060606 DQG
105 VSHFWURVFRS\ GDWD LQFOXGLQJ RQHGLPHQVLRQDO + & DQG &'(37 DV ZHOO DV
WZR GLPHQVLRQDO ++ &26< ++72&6< ++ 52(6< +& +64& +&
+0%&VSHFWUD$PRQJWKHQHZO\LVRODWHGFRPSRXQGVDUHWZRGHULYDWLYHVRIGLIHUXOLFDFLG
SURYLVLRQDOO\ FDOOHG SXOPRQDULRVLGH $ 0Z &+2 DQG SXOPRQDULRVLGH
%0Z&+27KHFKDUDFWHULVWLFVWUXFWXUDOIHDWXUHRISXOPRQDULRVLGHVLV
diferulic acid residue in aryltetralin form bridged via sucrose to either caffeic or ferulic acid
2UKDPQRS\UDQRVLGH
Key words: 3XOPRQDULDRI¿FLQDOLV, Boraginaceae, pulmonariosides
Acknowledgement: This work has been supported by a grant of the National Science Centre,
3RODQG1R'1=

GENUS THYMUS IN BULGARIA – A NEW PROJECT

AIMED AT REVEALING OF SPECIES’ METABOLITE
PROFILE AND GENETIC DIVERSITY
Ina Aneva1, Peter Zhelev2, Kalina Alipieva3, Vassya Bankova3,
Strahil Berkov1, Kalina Danova3, Marina Dimitrova1, Iva Doycheva1,
Ivan Evtimov2, Katya Georgieva1, Kristina Georgieva1, Tsvetinka
Grozdanova3, Vladimir Ilinkin1, Viktoriya Ivanova3, Todor Karakiev,
Teodor Nedelin2, Milena Nikolova1, Rozaliya Nikolova1, Milena Popova3,
Boriana Sidjimova1, Marina Stanilova1, Stoyan Stoyanov1, Milka
Todorova3, Boryanka Traikova1$QWRDQHWD7UHQGD¿ORYD3, Elina Yankova1

2
3
Genus Thymus FRPSULVHV PRUH WKDQ VSHFLHV RI SHUHQQLDO KHUEDFHRXV RU IUXWLFRVH SODQWV
FODVVL¿HGLQWRHLJKWVHFWLRQV7RWDORIVSHFLHVZLWKQXPHURXVVXEVSHFLHVDQGYDULHWLHVDUHOLVWHGLQ
)ORUD(XURSDHD7ZHQW\VSHFLHVRFFXUQDWXUDOO\LQ%XOJDULD2QHRIWKHPLVHQGHPLFWRWKHFRXQWU\
(Thymus perinicus 9HOHQ-DODV IRXUDUHHQGHPLFVWRWKH%DONDQ3HQLQVXODT. albanus+HLQU
%UDXQ H[:HWWVW T. comptus *ULVHE T. longedentatus 'HJHQ 8UXP 5RQQLJHU T. stojanovii
Degen) and other three are sub-endemics (T. atticus ýHODN., T. moesiacus Velen., T. sibthorpii Benth.
The studies on the genetic variation at species and within-species level will help the elucidation of
some unresolved taxonomic issues and at the same time will serve as characteristics of the genetic
UHVRXUFHVRIWKHVSHFLHV7KHVSHFLHVRIJHQXVThymusSURYRNHVXEVWDQWLDOLQWHUHVWZRUOGZLGHIURP
phytochemical point of view, due to their diverse biological activities with potential for application
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main objective of the present project is to perform a complete genetic and phytochemical study on
the species of genus ThymusLQ%XOJDULD'LVWLQJXLVKLQJWKHWD[DDFFRUGLQJWRWKHLUPRUSKRORJ\
chorology, ecological and genetic characteristics, metabolite content, essential oil composition,
expression of antioxidant activity will allow composition of a general picture of the species of genus
ThymusLQ%XOJDULD5HVXOWVRIWKHVWXGLHVZLOOEHDEDVLVIRUGHWHUPLQLQJWKHSODFHRIHDFKVSHFLHV
ZLWKLQWKHJHQHUDOVFKHPHRIWKHJHQXV7KHLQIRUPDWLRQJDWKHUHGZLOOVHUYHDVDVFLHQWL¿FEDVLVIRU
the initial stages of cultivation of the prospective (with best phytochemical characteristics) species,
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Key words: Thyme, diversity, taxonomy, active compounds


BIOLOGICAL ACTIVITY AND TOXICITY (PART II)

NOVEL EXTRACTION TECHNOLOGIES FOR MEDICAL

AND AROMATIC PLANTS
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University of Zagreb, Faculty of Food Technology and Biotechnology, Department of

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The components of medicinal and aromatic plants are used in many areas of food industry
(nutraceuticals, food supplements), pharmaceutical industry (herbal medicinal products and
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in the isolation of active components from medicinal and aromatic plants using various
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extraction and microwave assisted extraction enhance the mass transfer, reduce the extraction
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Key words:XOWUDVRXQGH[WUDFWLRQRSWLPL]DWLRQELRDFWLYHFRPSRXQGV

THE INVESTIGATION OF BIOLOGICAL ACTIVITY

OF SEVERAL WALNUT (JUGLANS REGIA L.) SEPTUM
EXTRACTS
Marius Emil Rusu1, Andrei Mocan2, Anca Pop3, Ionel Fizesan3, Ana-
Maria Gheldiu1, Ioan Tomuta1, Laurian Vlase1, Daniela-Saveta Popa3

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2
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Medicine and Pharmacy, Cluj-Napoca, Romania
3
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and Pharmacy, Cluj-Napoca, Romania
The increasing use of healthy unsaturated fats, more prone to oxidation, the understanding
of the pathology of oxidative stress in the organisms, and the negative side-effects of some
synthetic antioxidant compounds point to the research of natural antioxidants of plant origin
ZKLFK FRXOG EH XVHG LQ IRRG DQGRU SKDUPDFHXWLFDO LQGXVWU\ 7R LQYHVWLJDWH WKH in vitro
biological activity of walnut septum extracts with high content in antioxidants, and to determine
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DQGF\WRWR[LFLW\RQDOOWKUHHFDQFHUFHOOOLQHV7KHVDPHF\WRWR[LFGRVHUHVSRQVHFXUYHZDV
REVHUYHGIRU$DQG+HS*DIWHUKDQGKH[SRVXUHFHOOYLDELOLW\GHFUHDVHGE\
DQGUHVSHFWLYHO\2Q0&)DKRUPHWLFUHVSRQVHZDVREVHUYHGVLJQL¿FDQWLQFUHDVH
in cellular metabolic activity at intermediate concentration levels and cytotoxic activity at
WKH KLJKHVW WHVWHG GRVH 7KH HVWURJHQLF DFWLYLW\ RI H[WUDFWV ZDV FRQ¿UPHG 2XU UHVHDUFK
demonstrates that walnut septum can be a source of bioactive compounds for biopharma or
food industry and assert the extension of the investigation in further evaluating the metabolic
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Key words:ZDOQXWVHSWXPHQ]\PHVF\WRWR[LFLW\FDQFHU

THIN LAYER DRYING BEHAVIOUR OF FOLIUM

CUM FLORE CRATAEGI FROM DIFFERENT PLANT
FLOWERING STAGES
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+RKHQKHLP6WXWWJDUW
Crataegus/KDZWKRUQVLVDZHOOGH¿QHGJHQXVWUDGLWLRQDOO\GH¿QHGDVEHORQJLQJWRWKHWULEH

CrataegeaeVXEIDPLO\0DORLGHDHRI5RVDFHDH7KHQXPEHURICrataegus species may vary
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behaviour of Folium cum Flore Crataegi was investigated by using a dynamic vapour sorption
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isotherm would determine the optimal time of drying when using assisted air movement
GU\HUV 'U\LQJ RI Crataegus fresh material for determination of optimal drying conditions
were investigated for the mixture (Folium cum Flore Crataegi) material at air temperature of
DQG&VSHFL¿FKXPLGLW\RIJNJDQGDLUÀRZRIPVDQGPDWXULW\
KDUYHVWLQJEDWFKHV/RDGLQJGHQVLW\RIIUHVKOHDYHVDQGÀRZHUVRINJP-2, which develops
DQLQLWLDOOD\HUGHSWKRIDERXWPPZDVFDOFXODWHGDVRSWLPDOGHQVLW\EDVHGRQSUHYLRXV
H[SHULHQFHZLWKVLPLODUSURGXFWV,WLVNQRZQWKDWSODQWPDWHULDOVH[SRVHGWRZDWHUDFWLYLW\
EHORZ RU UHODWLYH KXPLGLW\ FDQ EH VWRUHG VDIHO\ DW DPELHQW WHPSHUDWXUH )URP WKH
measured data, it can be observed that drying CrataegusDWDPRLVWXUHFRQWHQWRIJJGE
0&DQGVWRULQJDWWHPSHUDWXUHRI&LVVDIHDQGZKHQVWRULQJDWKLJKHUWHPSHUDWXUHV
VXFKDV&LWLVQHFHVVDU\WRGU\DW0&JJGE0&
Key words: Crataegus, medicinal plants, drying behaviour, thin layer, sorption isotherm

CURRENT APPROACH AND CHALLENGE FOR

THE PROFILING OF GLUCURONIDE OLEANANE-
TYPE TRITERPENOID CARBOXYLIC ACID 3,
28-O-BIDESMOSIDES (GOTCAB) SAPONINS. A CASE
STUDY OF GYPSOPHILA L. (CARYOPHYLLACEAE)
Reneta Gevrenova1%DUEDUD.RáRG]LHM2, Max Henry3

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France
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among the highest glycosylated triterpenoid saponins widespread in the taxonomic groups
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and G. glomerate 3DOOH[$GDPVDQGFXOWLYDWHGG. scorzonerifolia6HUG. acutifolia 6WHYH[
Spreng, G. altissima/G. paniculate/*SDFL¿FD.RPG. oldhamiana0LTG. zhegualensis
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The principal compound analysis (PCA) grouped cultivated G. paniculata, G. oldhamiana and
G. zhegualensisLQDZHOOGH¿QHGFOXVWHU7KLVH[DPSOHRIDVVHVVLQJWKH*27&$%FRXOGEHXVHG
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Key words:WULWHUSHQRLGVDSRQLQV*27&$%Gypsophila

STATE OF THE ART METHOD ON THE BASE OF GC-

ORBITRAP-HRMS FOR SESQUITERPENE LACTONES
IN ARNICA MONTANA L.
Vessela Balabanova1, Niko Benbassat1, Yulian Voynikov2, Reneta
Gevrenova1, Dimitrina Zheleva-Dimitrova1

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Bulgaria
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Arnica montana/ÀRZHUVKDYHDORQJKLVWRU\DVKHUEDOPHGLFLQHVIRUH[WHUQDOXVHRQLQMXULHV
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magnetic resonance methods for the analysis of A. montana 67/V KDYH EHHQ GHYHORSHG
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Key words: Arnica montanaVHVTXLWHUSHQHODFWRQHV*&2UELWUDS+506
$FNQRZOHGJHPHQW7KHVWXG\ZDV¿QDQFLDOO\VXSSRUWHGE\*UDQW'IURPWKH
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BIOINFORMATIC INSIDE ON PORTULACA OLERACEA

L. (PURSLANE) WITH BULGARIAN AND GREEK
ORIGIN
Vessela Balabanova1, Reneta Gevrenova1, Dimitrina Zheleva-
Dimitrova1, Iassen Hristov2, Paulina Sugareva2, Valentin Lozanov2

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Bulgaria
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Bulgaria
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Component Analysis (PCA) combined with both descriptive and differential analyses were
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times, comparison with commercial standards and literature data these secondary metabolites
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&RPELQLQJ/&06GDWDZLWKPXOWLYDULDWHVWDWLVWLFDODQDO\VLVZDVSURYHQHIIHFWLYHLQVWXG\LQJ
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Key words: Portulaca oleraceaELRLQIRUPDWLFPHWKRGV/&±+5$06VHFRQGDU\

metabolites

OPTIMAL EXPERIMENTAL DESIGN DEVELOPED TO

PREPARE WALNUT (JUGLANS REGIA L.) SEPTUM
EXTRACTS WITH HIGH CONTENT OF BIOACTIVE
COMPOUNDS
Marius Emil Rusu1, Andrei Mocan2, Ana-Maria Gheldiu1, Daniela-
Saveta Popa3,RDQ7RPX‫܊‬D1, Laurian Vlase1

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Romania
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3
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experimental design with optimum extraction conditions based on which septum extracts rich
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FRQGHQVHGWDQQLQVDQGDQWLR[LGDQWFDSDFLW\GHWHUPLQHGE\7($&DVVD\7KHSK\WRFKHPLFDO
SUR¿OHRIO\RSKLOL]HGH[WUDFWVREWDLQHGE\WXUERH[WUDFWLRQZDVIXUWKHUGHWHUPLQHGE\/&06
MS analysis of individual polyphenolic compounds and lipophilic compounds: phytosterols
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K\GURSKLOLFDQGOLSRSKLOLFELRDFWLYHFRPSRXQGV7KHSK\WRFKHPLFDOSUR¿OHRIWKHDQDO\VHG
extracts proves that walnut septum can be a valuable source of biologically active compounds
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Key words: Juglans regia/VHSWXPH[WUDFWVH[SHULPHQWDOGHVLJQELRDFWLYHFRPSRXQGV

ESSENTIAL OIL COMPOSITION OF TANACETUM

PARTHENIUM L. FROM EASTERN BLACK SEA REGION,
TURKEY
Hakan Ozer1, Furkan Coban1, Ebru Mete2

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Feverfew (Tanacetum parthenium / EHORQJLQJ WR WKH IDPLO\ $VWHUDFHDH LV D SHUHQQLDO
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DUWKULWLVVWRPDFKDFKHVWRRWKDFKHVLQVHFWELWHVLQWUDGLWLRQDOPHGLFLQH1RZDGD\VLWVGULHG
herbal preparations in capsule and tablets has been sold as food supplement products and
GLVHDVHSUHYHQWLRQLQZRUOGPDUNHWV/LWHUDWXUHRQWKHHVVHQWLDORLOFRPSRVLWLRQRITanacetum
partheniumLVYHU\OLPLWHGLQ7XUNH\DQGLWVHVVHQWLDORLOFRPSRVLWLRQFDQYDU\ODUJHO\ZLWK
GLIIHUHQW HFRORJLFDO FRQGLWLRQV 7KHUHIRUH ZH LQYHVWLJDWHG WKH HVVHQWLDO RLO FRPSRVLWLRQ RI
Tanacetum parthenium FROOHFWHG IURP *XPXVKDQH SURYLQFH 7XUNH\ ,n this stXdy forty
foXr Fomponents representinJ of the essential oil of aerial parts of Tanecatum
parthenium were obtained by hydrodistillation and examined by *& and *&06 7KHPDLQ
FRPSRQHQW ZDV FDPSKRU DQG RWKHU PDMRU FRPSRQHQWV ZHUH JXDLRO
FDU\RSK\OOHQH R[LGH ERUQ\O DFHWDWH (YHUEHQRO FDPSKHQH
DQG ĮFDGLQRO 3UHVHQFH RI FDPSKRU KLJKOLJKW WKH SRWHQWLDO RI WKLV SODQW
as a drug source in the alternative medicine due to its antiseptic, antipruritic, aphrodisiac,
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Key words: feverfew, Asteraceae, essential oil, camphor, GC-MS

AFLATOXIGENIC STRAINS ISOLATED FROM

MEDICINAL HERBS
Cornelia Mircea1, Cristina Tuchilus2, Oana Cioanca3, Cristina Iancu1,
Ursula Stanescu3, Gabriela Tatarînga4, Monica Hancianu3

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RI3KDUPDFHXWLFDO%LRFKPLVWU\DQG&OLQLFDO/DERUDWRU\8QLYHUVLWDWLL6WUHHW,DVL
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VWDELOLW\RIH[WUDFWVSUHSDUHGIURPWKHP7KHDLPRIRXUVWXG\ZDVWRDVVHVVWKHOHYHORIIXQJDO
FRQWDPLQDWLRQ RI VDPSOHV RI PHGLFLQDO KHUEV DQG WR HYDOXDWH WKH DELOLW\ RI Aspergillus
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DJDUIRUWRWDOJHUPV3HWULGLVKHVZHUHLQFXEDWHGGD\VDW&IRUWRWDOJHUPVDQGGD\VDW
&IRUIXQJLGHYHORSPHQW7KHQXPEHURIFRORQ\IRUPLQJXQLWV&)8H[SUHVVHGLQ&)8J
KDVEHHQGHWHUPLQHGDIWHULQFXEDWLRQ)RUIXQJLWKHQXPEHURI&)8JZDVXQGHUWKHOLPLWRI
JDQGUDQJHGEHWZHHQ&)8J&KDPRPLOODHÀRV6DQG6DQG&)8J
(Urticae herba ±6)XQJLUHSUHVHQWHGRYHURIWRWDOJHUPVIRUDOOVDPSOHVWKDWZHUH
DQDO\VHG)URPAspergillus genus, Aspergillus nigerZDVWKHPRVWIUHTXHQWO\LGHQWL¿HGDQG
Theae foliumZDVWKHPRVWFRQWDPLQDWHGVDPSOHZLWKWKLVVWUDLQAspergillus niger isolated
IURPVDPSOHVKDVWKHDELOLW\WRSURGXFHDÀDWR[LQVZKHQVDPSOHKXPLGLW\DQGHQYLURQPHQWDO
WHPSHUDWXUHDUHLQFUHDVHG$ÀDWR[LQ%ZDVLGHQWL¿HGE\7/&DQGTXDQWL¿HGE\+3/&DIWHU
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sample (&KDPRPLOODHÀRV±6DQGȝJJVDPSOH&KDPRPLOODHÀRV6
Key words: fungi, contamination, medicinal herbs

COMMON JUNIPER (JUNIPERUS COMMUNIS L.) IN

SLOVAKIA
Ivan Salamon
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Common Juniper (Juniperus communis/LVVKUXERUVPDOOWUHHIWKLJK%DUNLVUHGGLVK

EURZQVKUHGGLQJRIILQSDSHU\SHHOV/HDYHVQHHGOHWDSHUWRDVSLQ\WLSLQZKRUOVRIV
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RI WKLV 6ORYDNLDQ QDWLRQDO OLTXRU GLVWLOOHU FRPSDQLHV SUHIHU WKH MXQLSHU IUXLWV ZLWK WKH KLJK
SLQHQHFRQWHQWVDVGRQRUVRIDURPDRGRUDQGORZHUHVVHQWLDORLOTXDQWLW\6DPSOHVRIMXQLSHU
IUXLWVZHUHFROOHFWHGIURPGLIIHUHQWORFDWLRQVLQ6ORYDNLDDQGWKHFRQWHQWRIHVVHQWLDORLO
DQGWKHLU*&SUR¿OHVZHUHGHWHUPLQHG7KHFRQWHQWRIHVVHQWLDORLOVUDQJHGEHWZHHQ
DQG7KHTXDOLWDWLYHTXDQWLWDWLYHFKDUDFWHULVWLFVRIWKHMXQLSHUHVVHQWLDORLOZHUH
SUHVHQWHGIRXUPDLQFRPSRQHQWVĮSLQHQH±VDELQHQH±
P\UFHQH±DQGFDU\RSK\OOHQH±
Ecological diversity of sites, where juniper occurs, its geological age tertiary resulted in great
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Key words: Juniper, GC, chemotypes
Acknowledgement: This research was supported by the Slovak Research and Development
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(Juniperus communis L.) for the production of fruits”.

TARGETED METABOLITE PROFILING TO SUPPORT

CHEMICAL AND BIOCHEMICAL CHARACTERISATION
OF MEDICINAL AND AROMATIC PLANTS (MAPS)
Stefan Martens1, Eirini Sarrou2, Paschalina Chatzopoulou2

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Cultivation of medicinal and aromatic plants (MAPs) is of growing interest to guarantee

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pharmaceuticals, nutraceuticals, veterinary medicine), but also to protect natural habitats
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increased interest in MAPs is considerably associated with naturally occurring antioxidants
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biosynthesis) in plants, especially in non-model or crop plants such as MAPs, is a research
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RI0$3VDUHWKHPRVWLPSRUWDQWFRPSRXQGVNQRZQIRUWKHLUDQWLR[LGDQWSURSHUWLHVDQGDUH
RIWHQLIQRWLGHQWL¿HGDVDFWLYHSULQFLSOHXVHGDVPDUNHUFRPSRXQGVLQTXDOLW\DVVHVVPHQWRI
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EHDSSOLHGIRUWKHGHYHORSPHQWRIQHZ'1$PROHFXODUPDUNHUV613VFKLSFRQWDLQLQJWKH
VHTXHQFHVRINH\JHQHVLQYROYHGLQWKHPHWDEROLFSDWKZD\VRILQWHUHVWFRXOGEHXVHGLQIXWXUH
EUHHGLQJSURJUDPVWRVFUHHQSRSXODWLRQVVHJUHJDWLQJWKHWDUJHWWUDLWDQGWRSURGXFHDOLQNDJH
PDS8VLQJWKHVHWRROVLPSURYHGFXOWLYDUVRIGLIIHUHQW0$3VFDQEHJHQHUDWHGLQFOXGLQJD
VROLGTXDOLW\FRQWUROWKURXJKRXWWKHSURGXFWLRQSURFHVV
Key words: metabolite analysis, breeding, MAPs

AN IN VITRO STUDY ON COMBRETUM

QUADRANGULARE Kuz., VERNONIA AMYGDALINA Del.
AND CLERODENDRUM INERME (L.) Gaertn. PLANT
63(&,(6)5207+(6287+2)9,(71$0)25Į
-AMYLASE INHIBITORY ACTIVITY
Le Viet Dung, Le Van Minh
Vietnam National Institute of Medicinal Materials
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drug or diet to delay the production or absorption of glucose by inhibiting carbohydrate-
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of use by Vietnamese people in the South, we have carried out an in vitro study on Combretum
quadrangulare .X] Vernonia amygdalina 'HO DQG Clerodendrum inerme / *DHUWQ IRU
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chloroform, ethyl acetat, n-butanol, water) from the leaves of the above plant species have been
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LQKLELWRU\ DFWLYLW\ )RU WKH OHDYHV RI Combretum quadrangulare, the strongest inhibition
ZDV DWWDLQHG E\ WKH QKH[DQH IUDFWLRQ ZLWK DQ ,& YDOXH RI J PL )RU WKH
OHDYHV of Vernonia amygdalina and Clerodendrum inerme, the chloroform fractions
showed the PRVW SRZHUIXO ZLWK ,& YDOXHV RI J PL DQG J PL UHVSHF
WLYHO\0HDQZKLOHWKH ,& YDOXH RI DFDUERVH ZDV J PL 7KH UHVXOWV VXJJHVWHG
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Key words: Combretum quadrangulare.X]Vernonia amygdalina'HOClerodendrum

inerme/*DHUWQĮDP\ODVHLQKLELWLRQ

PRELIMINARY DATA ON THE IMPACT OF SORBUS

AUCUPARIA L. BARK EXTRACT ON IN VITRO CELL
VIABILITY
Gabriela Vochita1, Cosmin-Teodor Mihai1,2, Pincu Rotinberg1, Elena
Ionescu3,4, Carmen-Elena Tebrencu3,4, Ruxandra-Mihaela Cretu5,
Daniela Gherghel1

Institute of Biological Research Iasi, branch of National Institute of Research and
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Neamt, Romania
Polyphenol-rich extracts obtained from different vegetable sources are currently under
investigation for their use as alternative therapeutic compounds for different diseases, the most
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RIYLDELOLW\DQGUHVSHFWLYHO\ZDVUHJLVWHUHGDWGRVHVRIDQGJP/
UHVSHFWLYHO\7KHF\WRWR[LFSRWHQWLDOZDVFRUUHODWHGZLWKWKHSRO\SKHQROLFFRQWHQWRIURZDQ
EDUNH[WUDFWVZKLFKGHSHQGVRQWKHH[WUDFWLRQFRQGLWLRQVDKLJKHU\LHOGRIH[WUDFWLRQEHLQJ
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SUHOLPLQDU\GDWDFRQ¿UPWKHF\WRWR[LFSURSHUWLHVRIWKHSRO\SKHQROLFK\GURDOFRKROLFH[WUDFWV
The further investigations will be employed in order to prove the reproducible character of the
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interaction the polyphenolic phytoextracts with the apoptosis process, cell cycle, healing
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Key words: Sorbus aucuparia/EDUNH[WUDFWVYLDELOLW\F\WRWR[LFLW\077
Acknowledgement: This work was supported by a grant of the Romanian National Authority
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PHENOLIC CONTENT AND PROFILE OF THREE

ARTEMISIA SPECIES FROM REPUBLIC OF MOLDOVA
Camelia P. Stefanache1, Valentin Grigoras1, Radu Necula1,2, Adriana
Trifan3, Oana Bujor1, Nina Ciocarlan4, Xavier Simonnet5

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Botanical Garden (Institute) of ASM, Chisinau, Republic of Moldova
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7KHDLPRIWKHSUHVHQWVWXG\ZDVWRDVVHVVWKHSKHQROLFFRQWHQWDQGSUR¿OHRIArtemisia species
IURP5HSXEOLFRI0ROGRYD:HIRFXVHGRQA. annua /A. absinthium/DQGA. lerchiana
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A. annuaOHDYHVKDUYHVWHGDWSUHÀRZHULQJVWDJHDQGA. absinthium and A. lerchiana herba
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FRPSDULVRQZLWKDXWKHQWLFVWDQGDUGV$PRQJWKHWKUHHArtemisia species in the study, A. annua
KDGWKHKLJKHVWFRQWHQWRISKHQROLFFRPSRXQGVPJ*$(JGU\
extract), followed by A. lerchianaPJ*$(JGU\H[WUDFWDQG
A. absinthiumPJ*$(JGU\H[WUDFW7KH+3/&'$'DQDO\VLV
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dry plant material in A. annua±PJJLQA. absinthiumDQG±PJJ
in A. lerchiana&KORURJHQLFDQGFDIIHLFDFLGVDORQJVLGHÀDYRQRLGVK\SHURVLGHLVRTXHUFLWULQ
DQGOXWHROLQJOXFRVLGHZHUHLGHQWL¿HGLQWKHArtemisiaH[WUDFWV$PRQJWKHWKUHHArtemisia
species, A. lerchianaKDGDPRUHFRPSOH[ÀDYRQRLGVSHFWUXP7KHKLJKSKHQROLFFRQWHQWRI
these Artemisia species suggests their possible use in the food and pharmaceutical industries
DVDQLPSRUWDQWVRXUFHRIDQWLR[LGDQWV
Key words: A. annua, A. absinthium, A. lerchiana, pytochemical screening

VARIABILITY AND COMPOSITION OF ESSENTIAL

OIL CONTENT OF SELECTED SPICES (OCIMUM
BASILLICUM L., SATUREJA HORTENSIS L. AND
MAJORANA HORTENSIS Moench.)
-DQD%HQHãRYi+HOHQD3OXKiþNRYi
Department of Crop Science, Breeding and Plant medicine, Faculty of Agronomy,

0HQGHO8QLYHUVLW\LQ%UQR=HPČGČOVNi%UQR&]HFK5HSXEOLF
The thesis Variability and composition of essential oil content of selected spices (Ocimum
basillicum /Satureja hortensis/DQGMajorana hortensis0RHQFKGHDOVZLWKSODQWVSHFLHV
RI ZKRVH PDLQ LQJUHGLHQW LV RLO 7KH SURFHVV RI WKH LVRODWLRQ RI HVVHQWLDO RLOV IURP SODQWV
LQGLYLGXDO FRPSRQHQWV RI HVVHQWLDO RLOV WKHLU YDULDELOLW\ DQG XVH DUH GHVFULEHG 6HOHFWHG
UHSUHVHQWDWLYHVRIWKHVRFDOOHGJUHHQVSLFHVEHORQJWRWKHPLQWIDPLO\/DPLDFHDHZKRVH
W\SLFDOIHDWXUHLVYHU\KLJKFRQWHQWRIHVVHQWLDORLOV,QWKHSUDFWLFDOSDUWWKHIROORZLQJVSHFLHV
are evaluated: basil (Ocimum basilicum), summer savory (Satureja hortensis/DQGPDUMRUDP
(Origanum majoranaV\QMajorana hortensis(VVHQWLDORLOFRQWHQWZDVGHWHUPLQHGLQHDFK
species by steam distillation and then the composition of essential oils was determined by gas
FKURPDWRJUDSK\
Key words: essential oils, basil, marjoram, savory, steam distillation, gas chromatography

MAIN PHENOLIC COMPOUNDS AND ANTIOXIDANT

ACTIVITY OF METHANOLIC EXTRACT OF CARDUUS
KERNERI Simonkai SSP. $8675225,(17$/,6 Franco
INFLORESCENCES
Ivanka Dimitrova-Dyulgerova1, Iliya Zhelev2

3ORYGLY8QLYHUVLW\?´3DLVLL+LOHQGDUVNL)DFXOW\RI%LRORJ\'HSDUWPHQWRI%RWDQ\DQG
PHWKRGVRIELRORJ\WHDFKLQJ7]DU$VVHQVWUHHW3ORYGLY%XOJDULD
2
Medical University of Varna, Faculty of Pharmacy, Department of Pharmaceutical sciences,
0DULQ'ULQRYVWU9DUQD%XOJDULD
3KHQROLF DFLG DQG ÀDYRQRLG SUR¿OHV RI WKH %DONDQ HQGHPLF SODQW VSHFLHV Carduus kerneri
6LPRQNDLVVSaustro-orientalis)UDQFRZHUHLQYHVWLJDWHGIRUWKH¿UVWWLPH7HQSKHQROLFDFLGV
DQGHLJKWÀDYRQRLGVZHUHLGHQWL¿HGDQGTXDQWL¿HGLQWKHLQÀRUHVFHQFHVE\KLJKSHUIRUPDQFH
OLTXLGFKURPDWRJUDSK\7KHPDLQÀDYRQRLGVZHUHIRXQGWREHOXWHROLQJ
gGZDSLJHQLQJJGZK\SHURVLGJJ dw), and the main
SKHQROLFDFLGVZHUHVLQDSLFDFLGJJGZDQGFKORURJHQLFDFLG
J J GZ 7KH DQWLR[LGDQW DFWLYLW\ RI PHWKDQROLF H[WUDFW RI LQÀRUHVFHQFHV KDV EHHQ
investigated, employing four different established testing systems: scavenging activity on
GLSKHQLOSLFU\OK\GUD]\O '33+ ¶D]LQRELVHWK\OEHQ]RWKLD]ROLQHVXOIRQDWH
$%76 UDGLFDO FDWLRQ GHFRORUL]DWLRQ DVVD\ IHUULF UHGXFLQJ DQWLR[LGDQW SRZHU )5$3 DQG
FRSSHUUHGXFWLRQDQWLR[LGDQWDVVD\V)5$37KHKLJKHVWDQWLR[LGDQWDFWLYLW\YDOXHV
mMTE gZHUHPHDVXUHGE\WKH$%76DVVD\DPRQJDOOSHUIRUPHGPHWKRGV
Key words: Carduus kerneri+3/&ÀDYRQRLGVSKHQROLFDFLGVDQWLR[LGDQWDFWLYLW\

MORPHOLOGIC AND CHEMICAL PROFILE OF

TWO AJUGA SPECIES CULTIVATED IN NORTHERN
ROMANIA
Oana Cioanca1, Cristina Cerasela Mircea (Arsene)1, Ana Flavia Burlec1,
Adrian Spac1, Andreea Corciova1, Elvira Gille2, Silvia Robu3, Monica
Hancianu1

³*ULJRUH73RSD´8QLYHUVLW\RI0HGLFLQHDQG3KDUPDF\8QLYHUVLWă‫܊‬LL6WUHHW,D‫܈‬L
Romania
2
³'XQDUHDGH-RV´8QLYHUVLW\)DFXOW\RI0HGLFLQHDQG3KDUPDF\*DODWL5RPDQLD
3
1DWLRQDO,QVWLWXWHRI5 'IRU%LRORJLFDO6FLHQFHV³6WHMDUXO´%LRORJLFDO5HVHDUFK&HQWUH
Piatra Neamt, Romania
Ajuga genevensis / DQG Ajuga reptans / DUH WZR PHGLFLQDO SODQWV XVHG LQ 5RPDQLDQ
WUDGLWLRQDOPHGLFLQHIRUWKHLUDQWLLQÀDPPDWRU\SURSHUWLHVIRUUKHXPDWLVPDQGJRXW7KH\DUH
DOVRFUHGLWHGZLWKDVWULQJHQWWRQLFDQGGLXUHWLFDFWLYLWLHV$OWKRXJKWKHVHVSHFLHVDUHFRPPRQ
IRU WKH ZLOG ÀRUD LQ RXU FRXQWU\ RXU LQYHVWLJDWLRQ ZDV FRQGXFWHG RQ VSHFLPHQV KDUYHVWHG
LQ 0D\ IURP WKH H[SHULPHQWDO ¿HOGV RI ³6WHMDUXO´ %LRORJLFDO 5HVHDUFK &HQWUH 3LDWUD
1HDPW5RPDQLD7KHLULQWURGXFWLRQLQFXOWXUHDVDVRXUFHRIELRDFWLYHFRPSRXQGVLVLQWHQGHG
IRUSKDUPDFHXWLFDOSXUSRVHV7KHUHIRUHWKHLQYHVWLJDWLRQRIWKHLUFKDUDFWHULVWLFVDQGEHQH¿WVLV
RIH[WUHPHLPSRUWDQFHIRUIXWXUHXVHLQSKDUPDFHXWLFDOSUHSDUDWLRQVDQGRUIRRGVXSSOHPHQWV
7KHDHULDOSDUWVFKDUDFWHULVWLFVZHUHREVHUYHGZLWKDQHOHFWURQLFPLFURVFRSHLQVHYHUDO¿QH
FRORXUHGVHFWLRQVIRUHDFKVSHFLHV0RUHRYHUWKHLUFKHPLFDOSUR¿OHZDVDVVHVVHGRQK\GUR
DOFRKROLF H[WUDFWV E\ VSHFWURSKRWRPHWU\ DQG WKLQ OD\HU FKURPDWRJUDSK\ WHFKQLTXHV 7KH
UHVXOWVFRQ¿UPHGWKHWD[RQRPLFDQGFKHPLFDOYDULDELOLW\EHWZHHQWKHLQYHVWLJDWHGVDPSOHV
The microscopic features (glandular and surface hairs, epidermis structure, the number of and
W\SHRIVWRPDWDVKRZHGVLPLODULWLHVDQGGLIIHUHQFHVDWWKHVDPHWLPH,QWHUPVRIFRPSRXQG
SUR¿OHWKHSRO\SKHQROVÀDYRQHVDQGSRO\SKHQROLFDFLGVZHUHULFKHULQAjuga reptans
PJDQGPJUHVSHFWLYHO\ZKHUHDVAjuga genevensisFRQWDLQHGKLJKHUTXDQWLWLHVRI
LULGRLGVPJDVFRPSDUHGWRPJ)XUWKHUUHVHDUFKLVVWLOOXQGHUJRLQJWRDVVHVV
WKHLQWHQVLW\RIWKHLUELRORJLFDOSUR¿OHLQQHXURLQÀDPPDWLRQPRGHORQODERUDWRU\UDWV
Key words: AjugaPLFURVFRS\FKHPLFDOSUR¿OHSRO\SKHQROVLULGRLGV

CONTENT OF BIOLOGICALLY ACTIVE COMPOUNDS

IN HYSSOPUS OFFICINALIS L. TRADITIONALLY
CULTIVATED, IN VITRO PROPAGATED AND PLANTS
FROM NATURAL HABITATS
Ira Stancheva, Maria Geneva, Marieta Hristozkova, Ely Zayova
The changes of biologically active substances in methanolic extracts from traditionally

cultivated +\VVRSXVRI¿FLQDOLV/SODQWVIURPVHHGVin vitro propagated and adapted under
¿HOG FRQGLWLRQV SODQWV DQG SODQWV IURP QDWXUDO KDELWDWV DUH FRPSDUHG 7KH FRQWHQW RI WKH
LQYHVWLJDWHGQRQHQ]\PDWLFORZPROHFXODUPHWDEROLWHV±ZDWHUDQGOLSLGVROXEOHPHWDEROLWHV
ZLWKDQWLR[LGDQWFDSDFLW\SKHQROVÀDYRQRLGVUHDFKHGWKHKLJKHVWYDOXHVLQWKHÀRZHUVDQG
leaves in the in vitroSURSDJDWHGDQGDGDSWHGXQGHU¿HOGFRQGLWLRQVSODQWV/HDIDQGÀRZHU
extracts of + RI¿FLQDOLV in all three plant breeding modes showed different antioxidant
potential, but the highest values were observed in in vitroSURSDJDWHGSODQWV'LIIHUHQFHVKDYH
been observed between the total content of essential oil and the components of essential oils
GHSHQGLQJRQWKHZD\RISODQWVFXOWLYDWLRQ+LJKHVWFRQWHQWRIHVVHQWLDORLOLQSHUFHQWDJHRIWKH
GUXJZDVPHDVXUHGLQSODQWVIURPQDWXUDOKDELWDWV(VVHQWLDORLOIURPSODQWVSURSDJDWHGIURP
VHHGVKDYHWKHKLJKHVWYDOXHVRIWKHVDELQHQ(VVHQWLDORLOGHULYHGIURPin vitro propagated
SODQWVKDYHWKHKLJKHVWFRQWHQWRISLQRFDPSKRQ2LOGHULYHGIURPQDWXUDOKDELWDWVSODQWVKDYH
WKHKLJKHVWFRQWHQWRIȕELQHQDQGSLQRFDPSHQ
Key words: +\VVRSXVRI¿FLQDOLV, antioxidant metabolites, essential oil
3URMHFW'1

INFLUENCE OF MEDICINAL AND AROMATIC PLANTS

ON EGG YOLK SENSORY TRAITS IN LAYING HEN
Ivica Kos1=ODWNR-DQMHþLü2.ODXGLMD&DURYLü6WDQNR3,YDQ9QXþHF1,
,YDQâLULü1, Toni Safner4'DOLERU%HGHNRYLü2

University of Zagreb, Faculty of Agriculture, Department of Animal Science and
7HFKQRORJ\6YHWRãLPXQVNDFHVWD=DJUHE&URDWLD
2
University of Zagreb, Faculty of Agriculture, Department of Animal Nutrition,
3

University of Zagreb, Faculty of Agriculture, Department of Plant Breeding, Genetics and
%LRPHWULFV6YHWRãLPXQVNDFHVWD=DJUHE&URDWLD
Many medicinal plants with naturally present pigments have a potential of replacing synthetic
SLJPHQWV XVHG IRU \RON FRORULQJ SURSHUWLHV LQ HJJ SURGXFWLRQ 7KHUHIRUH WKH DLP RI WKLV
research was to determine the effect of addition of basil (Ocimum basilicum / % SRW
marigold (&DOHQGXODRI¿FLQDOLV/&GDQGHOLRQ7DUD[DFXPRI¿FLQDOH:HEHUH[:LJJ'
and marigold (Tagetes erecta/0LQGLHWRQHJJ\RONVHQVRU\WUDLWV&RQWUROJURXSZDVIHG
ZLWKDVWDQGDUGFRPPHUFLDOGLHWIRUOD\HUVZLWKV\QWKHWLFSLJPHQWVIRU\RONFRORULQJLQFOXGHG
7ZROHYHOVDQGRIHDFKSODQWÀRZHU&'0RUKHUE%SRZGHUKDYHEHHQDGGHGIRU
ZHHNVLQWULDOGLHWVDQGWKHQVHQVRU\DQDO\VLVRIFRRNHGHJJ\RONVZDVSHUIRUPHG,QWRWDO
97 consumers among Faculty of Agriculture staff and students evaluated samples by using
SRLQW KHGRQLF VFDOHV IRU FRORU DURPD WH[WXUH ÀDYRU RIIRGRUV DQG RYHUDOO LPSUHVVLRQ
'LIIHUHQFHV RI PHGLDQ VFRUHV EHWZHHQ VDPSOHV ZHUH WHVWHG XVLQJ .UXVNDO:DOOLV UDQN VXP
WHVW)RUWUDLWVZKHUHVLJQL¿FDQWGLIIHUHQFHVEHWZHHQVDPSOHVZHUHFRQ¿UPHGGLIIHUHQFHRI
PHGLDQRIHDFKWUHDWPHQWDQGFRQWUROJURXSZDVWHVWHGXVLQJ6WHHOPHWKRG,WZDVIRXQGWKDW
DURPDWH[WXUHRIIRGRUVDQGRYHUDOOLPSUHVVLRQZHUHQRWVLJQL¿FDQWO\DIIHFWHGE\WKHDGGLWLRQ
RIPHGLFLQDODQGDURPDWLFSODQWVLQIHHG6LJQL¿FDQWO\KLJKHUUDWHG\RONFRORUVFRUHRIFRQWURO
JURXSDQGWUHDWPHQW0DWZHUHIRXQGZKHQFRPSDUHGWRWUHDWPHQWV%&DQG'DWERWK
OHYHOV)ODYRUVFRUHVRIDOOWUHDWPHQWVH[FHSWWUHDWPHQW0DWZHUHVLJQL¿FDQWO\ORZHUUDWHG
ZKHQFRPSDUHGWRFRQWUROJURXS$GGLWLRQRIFDOHQGXODDQGGDQGHOLRQÀRZHUSRZGHUDQGEDVLO
KHUESRZGHULQOD\HUVIHHGDSSHDUHGWREHLQDGHTXDWHIRUHJJ\RONVHQVRU\WUDLWV2QWKHRWKHU
hand, marigold is found to be a potential replacement for synthetic pigments with emphasis
RQIXUWKHUUHVHDUFK
Key words:0$3VVHQVRU\WUDLWVHJJ\RON

CONCERN ON THE PHARMACOPOEIA EXTRACTION

PROCEDURE FOR DETERMINATION OF ARBUTIN
CONTENT IN BEARBERRY LEAVES
*6WHINRY,&YHWNRYLNM.DUDQ¿ORYD9.HUOXNX0.DUDSDQG]RYD
S. Kulevanova
6NRSMH50DFHGRQLD
Arctostaphylos uva-ursi/6SUHQJIDP(ULFDFHDHDOVRNQRZQDVEHDUEHUU\RUEHDU¶VHDULV

a small procumbent woody groundcover shrub, widely distributed on a global level and used as
XULQDU\WUDFWDQWLVHSWLFDQGGLXUHWLFGXHWRWKHSUHVHQFHRIDUEXWLQDQGK\GURTXLQRQH7KHKHUE
FRQWDLQVK\GURTXLQRQHJO\FRVLGHVDUEXWLQPHWK\ODUEXWLQJDOOR\OGHULYDWLYHV
RIDUEXWLQDQGIUHHK\GURTXLQRQHDVGHFRPSRVLWLRQSURGXFWRIDUEXWLQ7KH
RI¿FLQDOKHUEDOVXEVWDQFHFRQVLVWVRIZKROHRUFXWGULHGOHDYHVDQGVKRXOGQRWFRQWDLQOHVVWKDQ
RIDQK\GURXVDUEXWLQDFFRUGLQJWRWKH(XURSHDQ3KDUPDFRSRHLD7KHFRQWHQWRIDUEXWLQ
FDQEHGHWHUPLQHGLQSODQWH[WUDFWVE\VSHFWURSKRWRPHWU\RUFDSLOODU\]RQHHOHFWURSKRUHVLVEXW
+3/&PHWKRGZDVIRXQGWREHWKHPRVWVXLWDEOHDQGLQFOXGHGLQWKH3K(XUPRQRJUDSK
6HYHUDOZLOGJURZLQJSRSXODWLRQVRIEHDUEHUU\IURP50DFHGRQLDZHUHDVVHVVHGRQDUEXWLQ
FRQWHQWDQGDOOWKHUHVXOWVZHUHDSSUR[LPDWHO\ORZHUWKDQWKHUHTXLUHGPLQLPXPRI
$FFRUGLQJWKHPHWKRGGHVFULEHGLQWKH3K(XUWKHZDWHUH[WUDFWZDVSUHSDUHGIURPGULHG
EHDUEHUU\ OHDYHV ([WUDFWLRQ SURFHGXUH FRPSULVHV WZR VWHSV ZDWHU PDFHUDWLRQ RI SRZGHUHG
KHUEDO GUXJ HDFK RI PLQXWHV KHDWHG RQ ZDWHU EDWK XQGHU UHÀX[ ($SDUW IURP WKLV
WZR LQGHSHQGHQWO\ GLIIHUHQW H[WUDFWLRQ SURFHGXUHV ZHUH DOVR SHUIRUPHG PLQXWHV ZDWHU
PDFHUDWLRQ RQ PDJQHWLF VWLUUHU RQ URRP WHPSHUDWXUH ( RU ERLOLQJ ( 2EWDLQHG UHVXOWV
revealed that the content of arbutin is much higher in the extract obtained by E2 but lower
LQ WKH H[WUDFW REWDLQHG E\ ( FRPSDUHG ZLWK WKH SKDUPDFRSRHLDV¶ PHWKRG (7KH UROH RI
LQFUHDVHGH[WUDFWLRQWHPSHUDWXUHLVTXHVWLRQDEOHWDNLQJLQWRFRQVLGHUDWLRQWKDWDUEXWLQKDVYHU\
JRRGVROXELOLW\LQZDWHURIJ/DWURRPWHPSHUDWXUH&DVGHWHUPLQHGXVLQJWKH
ÀDVNPHWKRG$RI&RPPLVVLRQ'LUHFWLYH((&
Key words: Arctostaphylos uva-ursi, sample-preparation, solubility, arbutin

INVESTIGATION OF THE ANTIBACTERIAL ACTIVITY

OF SOME ANTHOCYANIDIN-RICH EXTRACTS ON
CLINICAL ISOLATES OF UPEC
Remo Dorneanu1, Oana Cioanca1, Cornelia Mircea12DQD&KL¿ULXF2,
Cristina Tuchilus1, Cristina Iancu1, Ana Flavia Burlec1, Andreea
Corciova1, Ivan Salamon30RQLFD+ăQFLDQX1

³*ULJRUH73RSD´8QLYHUVLW\RI0HGLFLQHDQG3KDUPDF\
8QLYHUVLWă‫܊‬LL6WUHHW,D‫܈‬L5RPDQLD
2
Research Institute of the University of Bucharest, Bucharest, Romania
3
8QLYHUVLW\RI3UHVRYWK1RYHPEHU6WUHHW3UHVRY6ORYDN5HSXEOLF
Uropathogenic Escherichia coli (UPEC) is the most common cause of recurring urinary tract
LQIHFWLRQVZRUOGZLGH7KHXVXDOWUHDWPHQWLQFOXGHVDQWLELRWLFVWKDWVWRSWKHPXOWLSOLFDWLRQRI
SDWKRJHQV1HYHUWKHOHVVDFTXLUHGUHVLVWDQWVWUDLQVWKDWFDQDGDSWHDVLO\KDYHVSUHDGDOORYHU
WKHZRUOGGXHWRLUUDWLRQDOXVHRIDQWLELRWLFVDQGPDQ\SDWKRJHQVUHVLVWDQWWRÀXRURTXLQRORQHV
RUWKLUGJHQHUDWLRQFHSKDORVSRULQZHUHLGHQWL¿HGLQFOLQLFDOUHVHDUFK7KHUHIRUHWKLVVWXG\LV
part of a larger research that aims to identify natural compounds that can be used to prevent
or treat urinary tract infections, either on their own or as part of a complex treatment which
LQFOXGHVDQDQWLELRWLF$QWKRF\DQLQVDUHQDWXUDOFRPSRXQGVZHOOUHFRJQL]HGIRUWKHLUKHDOWK
EHQH¿WV 6HYHUDO DQWKRF\DQLQULFK H[WUDFWV O\RSKLOL]HG SRZGHUV IURP Aronia melanocarpa
/ ZHUH DVVHVVHG LQ WHUPV RI WKHLU DQWLPLFURELDO HIIHFW RQ FOLQLFDO LVRODWHV RI 83(& 7KH
FKHPLFDOSUR¿OHRIWKHH[WUDFWVZDVLGHQWL¿HGE\83/&WHFKQLTXHV7KHPLQLPDOLQKLELWRU\
FRQFHQWUDWLRQ0,&DQGPLQLPDOELR¿OPHUDGLFDWLRQFRQFHQWUDWLRQ0%(&RIWKHH[WUDFWV
against one standard (E. coli ATCC) and a few clinical isolated strains (E. coli E.
coliDQGE. coliZHUHGHWHUPLQHG7KHUHVXOWVLQGLFDWHGWKDWWKHH[WUDFWVKDYHD
JRRGDQWLEDFWHULDODFWLYLW\PJP/0,&RU0%(&1HYHUWKHOHVVWKHV\QHUJLVWLFHIIHFWV
ZHUH PRGHVW DQG D VOLJKW LQFUHDVH RI WKH JURZWK LQKLELWLRQ ]RQH GLDPHWHU IRU WKH DQWLELRWLF
QLWURIXUDQWRLQLPLSHQHPDQGQRUÀR[DFLQGLVNVVXSSOHPHQWHGZLWKWKHLQYHVWLJDWHGH[WUDFWV
ZDVQRWHG)XUWKHUVWXGLHVDUHQHFHVVDU\WRGUDZDFRQFOXVLRQUHJDUGLQJV\QHUJLVPEHWZHHQ
DQWLELRWLFDQGDQWKRF\DQLGLQULFKH[WUDFWVDQGLWVLPSRUWDQFH
Key words: UPEC, anthocyanins, Aronia melanocarpa, antimicrobial

MICROWAVE AND ULTRASOUND ASSISTED

EXTRACTIONS OF POLYPHENOLS FROM DANDELION
(TARAXACUM OFFICINALE (L.) Weber ex F.H. Wigg)
ROOT AND LEAF
'DQD7HQåHUD'DQLMHOD%XUVDü.RYDþHYLü0DWHD0LUNRYLü3UHGUDJ
3XWQLN9HULFD'UDJRYLü8]HODF
)DFXOW\RI)RRG7HFKQRORJ\DQG%LRWHFKQRORJ\8QLYHUVLW\RI=DJUHE3LHURWWLMHYD
Zagreb
Microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) as eco-

friendly procedures are widely employed in the analysis and the extraction of bioactive
FRPSRXQGVIURPSODQWV,QFRPSDULVRQWRWKHFRQYHQWLRQDOVROYHQWH[WUDFWLRQPHWKRG0$(
is more advanced as it heats the matrix internally and externally without a thermal gradient
thus target compounds can be extracted more effectively and protectively using less energy
DQG VROYHQW 0RUHRYHU WKH PDLQ DGYDQWDJHV RI XVLQJ 8$( LQFOXGHV PRUH HIIHFWLYH PL[LQJ
of matrix and solvent, faster mass transfer, reduced thermal and concentration gradients, and
OHVVHQHUJ\DQGVROYHQWFRQVXPSWLRQ+HQFHWKHDLPRIWKLVVWXG\ZDVWRLQYHVWLJDWH8$(DQG
MAE for isolation of total phenols from dandelion (7DUD[DFXPRI¿FLQDOH/:HEHUH[)+
:LJJURRWVDQGOHDYHV6HYHUDOSDUDPHWHUVVXFKDVHWKDQROFRQFHQWUDWLRQVYVYY
DQGWLPHPLQZHUHVWXGLHGIRUERWKWHFKQLTXHV0RUHRYHU0$(ZDVFRQGXFWHG
DW YDULRXV WHPSHUDWXUH & ZKLOH VRQLFDWLRQ ZDV SHUIRUPHG DW N+] DW
DQGDPSOLWXGHRIWKHIXOOSRZHU:LQWKHDPELHQWDWPRVSKHUH0$(KDVJLYHQ
WKH KLJKHVW \LHOGV IRU URRW DW & PLQ DTXHRXV HWKDQRO YY DQG IRU GDQGHOLRQ
OHDIDW&PLQDQGDTXHRXVVROXWLRQRIHWKDQROYY,QDGGLWLRQKLJKDPSOLWXGH
DQGKLJKSUHVHQFHRIHWKDQROLQWKHVROYHQWXQGHUPLQLPSURYHGVLJQL¿FDQWO\
WKH8$(SURFHVVRISRO\SKHQROVUHFRYHU\IURPURRWVZKLOH8$(SURFHVVZLWKDTXHRXV
HWKDQROYYDPSOLWXGHLQPLQZDVIRXQGDVWKHRSWLPDOIRUSRO\SKHQROVUHFRYHU\
IURPWKHGDQGHOLRQOHDI,QFRQFOXVLRQERWKWHFKQLTXHVFRQ¿UPHGVXLWDELOLW\IRUSRO\SKHQROV
UHFRYHU\IURPGDQGHOLRQURRWVDQGOHDYHV
Key words: microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE),

dandelion (7DUD[DFXPRI¿FLQDOH), polyphenols

EVALUATION OF THE ANTIOXIDANT POTENTIAL

OF ALCESEFOLISIDE FROM ASTRAGALUS
MONSPESSULANUS L. IN IN VITRO CELL SYSTEMS
Magdalena Kondeva-Burdina1, Georgi Popov2, Aleksandar Shkondrov3,
Ilina Krasteva3, Vassil Manov2

0HGLFDO8QLYHUVLW\RI6R¿D'XQDY6W6R¿D%XOJDULD
2
Department of Internal non-communicable Diseases, Pathology and Pharmacology, Faculty
RI9HWHULQDU\0HGLFLQH8QLYHUVLW\RI)RUHVWU\6W.OLPHQW2KULGVNL%OYG6R¿D
Bulgaria
3
'HSDUWPHQWRI3KDUPDFRJQRV\)DFXOW\RI3KDUPDF\0HGLFDO8QLYHUVLW\RI6R¿D'XQDY
6W6R¿D%XOJDULD
Astragalus monspessulanus/)DEDFHDHLVDFOXPSIRUPLQJSHUHQQLDOSODQWQDWLYHWRWKH

,EHULDQ3HQLQVXOD)UDQFH6ZLW]HUODQGWKH$SHQQLQH3HQLQVXODWKH%DONDQ3HQLQVXOD(DVWHUQ
(XURSH DQG 1RUWKZHVW $IULFD 3UHYLRXV VWXGLHV VKRZHG WKDW D SXUL¿HG VDSRQLQ IUDFWLRQ
REWDLQHGIURPWKHSODQWSRVVHVVHGF\WRWR[LFLW\LQ+HS*FHOOVDQGWKHQEXWDQROH[WUDFWRI
the overground parts exhibited hepatoprotective and antioxidant activities against in vitro and
in vivo &&OLQGXFHG DFXWH OLYHU GDPDJH FRPSDUDEOH WR WKDW RI VLO\PDULQ$ QHZ ÀDYRQRO
WHWUDJO\FRVLGHWZRQHZÀDYRDONDORLGVDQGWKHHLJKWNQRZQÀDYRQRLGVLHDODQJLÀDYRVLGH
DOFHVHIROLVLGHPDXULWLDQLQTXHUFHWLQȕURELQRELRVLGHFRVPRVLQHDSLJHQLQƍ2JOXFRVLGH
WULIROLQDQGUXWLQZHUHUHSRUWHGIURPWKHVSHFLHV7KHDLPRIWKHFXUUHQWVWXG\ZDVWRHYDOXDWH
WKH HIIHFW RI WKH UDUH ÀDYRQRLG DOFHVHIROLVLGH TXHUFHWLQ2Į/UKDPQRS\UDQRV\Oĺ
>Į/UKDPQRS\UDQRV\Oĺ@ȕ'JDODFWRS\UDQRVLGHXVLQJin vitroPRGHOVRIOLYHULQMXU\
In in vitroPRGHOVRI&&OLQGXFHGPHWDEROLFELRDFWLYDWLRQDQGW%X22+LQGXFHGR[LGDWLYH
VWUHVVLQLVRODWHGUDWKHSDWRF\WHVWKHÀDYRQRLGH[HUWHGDQWLR[LGDQWDQGF\WRSURWHFWLYHDFWLYLW\
VLPLODUWR6LO\ELQGLVFHUQHGE\SUHVHUYHGFHOOYLDELOLW\DQG*6+OHYHOVDVZHOODVE\GHFUHDVHG
/'+DFWLYLW\DQG0'$TXDQWLW\7KHHIIHFWVRIDOFHVHIROLVLGHZDVFRPPHQVXUDEOHZLWKWKRVH
RI6LO\ELQ$%
Key words: alcesefoliside, Astragalus monspessulanus, carbon tetrachloride, hepatotoxicity,

oxidative stress, phytochemicals
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&RXQFLODW0HGLFDO8QLYHUVLW\RI6R¿D

CHARACTERIZATION OF INVAZIVE PLANT SPECIES

FOR POSSIBLE USE IN PHYTOTERAPY
Valentin Grigoras1, Ruxandra-Mihaela Cretu1, Radu Necula1,2,
Georgiana-Luminita Gavril1, Elvira Gille1

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2
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PHGLFLQH LQ 5RPDQLD ,Q WKLV VWXG\ ZH VHDUFKHG IRU VRPH VROXWLRQV WR OLPLW WKH H[SDQVLRQ
of invasive plant species and also to determine the possible use of their active compounds
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7UDQV\OYDQLD5RPDQLD9DULRXVDVSHFWVKDYHEHHQVWXGLHGVXFKDVWKHVL]HRIDUHDVZLWKZLOG
populations, the vegetation time, the number of individuals per surface unit, the vegetative
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FRQWHQW RI DFWLYH SULQFLSOHV ZLWK DQWLR[LGDQW DFWLYLW\ SRO\SKHQROV ÀDYRQRLGV WULWHUSHQHV
HVVHQWLDORLODQGYRODWLOHIUDFWLRQVZDVGHWHUPLQHG7KHVHDQDO\VHVUHYHDOHGWKHSUHVHQFHRI
YDULDEOHDPRXQWVRIDFWLYHSULQFLSOHVVSHFLHVDQGHQYLURQPHQWGHSHQGHQW7KHSUHVHQFHRI
the germacren D could explain the invasive capacity of Impatiens glandulifera5R\OH
and Solidago gigantea $LW 7KH F\WRWR[LF DQG JHQRWR[LF FDSDFLW\ RI JHUPDFUHQ
'LVUHFRJQL]HGLQYDULRXVW\SHVRIHVVHQWLDORLOV$IHDWXUHFKDUDFWHULVWLFRIWKHHelianthus
tuberosus/LVWKHSUHVHQFHRISRO\SKHQROVLQDOOSODQWRUJDQVZLWKWKHKLJKHVWDPRXQWIRXQG
LQOHDYHVPJJDOOLFDFLGHTXLYDOHQWVSHUJGULHGZHLJKW2XUUHVHDUFKZLOOFRQWLQXH
RYHUWKHQH[WIHZ\HDUVWRHOXFLGDWHPRUHDVSHFWVRIWKHLQYDVLYHSODQWVSHFLHVLQ5RPDQLD
Key words: invasive plant species, biomass, active principles, phytotherapy
Acknowledgement: The authors wish to thank the BIODIVERS 2 National Project (PN 16-
190401) for the funding.

EVALUATION OF THE EDIBLE FLOWERS OF

MEDICINAL PLANTS
Tereza Muchová, Jarmila Neugebauerová
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7$&ZHUHPHDVXUHG$$ZDVGHWHUPLQHGE\+3/&7KHKLJKHVWDPRXQWVZHUHPHDVXUHGLQ
Primula elatior+LOOPJNJDQGWKHORZHVWLQLamium purpureum/
PJNJ 73& ZDV GHWHUPLQHG VSHFWURSKRWRPHWULFDOO\ ZLWK )ROLQ &LRFDOWHX UHDJHQW
The highest amounts were measured in Origanum vulgare / PJ *$(
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GHWHUPLQHGVSHFWURSKRWRPHWULFDOO\ZLWKVRGLXPFKORULGHDQGDOXPLQLXPQLWULGH7KHKLJKHVW
amounts were measured in Origanum vulgare/P0.$7NJDQGWKHORZHVW
in Centaurea cyanus / P0 .$7NJ 7$& ZDV GHWHUPLQHG E\ WKH '33+
PHWKRG7KHKLJKHVWDPRXQWVZHUHPHDVXUHGLQRehmannia glutinosa*DHUWQ6WHXG
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COMPARISON OF PHENOLIC COMPOSITION AND

BIOLOGICAL ACTIVITY OF COMMERCIALLY
AVAILABLE WHITE WINES ORIGINATING FROM
FRUŠKA GORA, SERBIA
'LDQGUD3LQWDü0DULMD/HVMDN1DWDãD6LPLQ'HMDQ2UþLü1HGD
0LPLFD'XNLü
Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences,

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wine phenolics that not only affect organoleptic properties but are also mainly responsible
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antioxidant activity was evaluated spectrophotometrically by measuring the reducing power
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nitric oxide radicals, and by inhibiting lipid peroxidation, while the neuroprotective effect was
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failed to inhibit lipid peroxidation, in other performed assays, the samples exhibited moderate
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commercial white wines, distinguishing them as a promising source of natural antioxidants and
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ANTINOCICEPTIVE ACTIVITY OF METHANOLIC

LEAVES EXTRACT OF CRATEVA ADANSONII DC. IN
ALBINO RAT
N. Ukwuani-Kwaja, T. A Omodele, S. Umar
Department of Biochemistry, Faculty of science, Kebbi State University of Science and

Technology, Aleiro, Kebbi state, Nigeria
Pain is the most common reason for physician consultation in most developed countries, but
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Previous studies have demonstrated that Crateva adansonii'&VWHPEDUNSURGXFHGDQDOJHVLF
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leaves extract of C. adansonii XVLQJ WDLOÀLFN DQG DFHWLF DFLGLQGXFHG ZULWKLQJ PHWKRGV
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administration of C. adansonii OHDYHV ZDV VLJQL¿FDQWO\ LQFUHDVHG S GLIIHUHQFH LQ WKH
pain reaction time and number of writhing respectively compared to the standard drug aspirin
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Key words: acetic acid, antinociceptive, Aspirin, Crateva adansoniiWDLOÀLFN

EXTRACTION OF PYRETHRINS FROM DALMATIAN

PYRETHRUM (TANACETUM CINERARIIFOLIUM /Trevir./
Sch. Bip.) BY MATRIX-SOLID PHASE DISPERSION
0DUWLQD%LRãLü1,QHV7RSDORYLü1'DULR'DELü1, Filip Varga2,3, Martina
Grdiša2,3

Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology,
8QLYHUVLW\RI=DJUHE0DUXOLüHYWUJ=DJUHE&URDWLD
2
Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb,
3
Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv),
Dalmatian pyrethrum (Tanacetum cinerariifolium 7UHYLU6FK%LS.) is a plant species of the

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potential of Dalmatian pyrethrum is measured by the content and composition of pyrethrins
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its insecticidal potential, content and composition of pyrethrins was assessed by matrix-solid
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Acknowledgment: This work has been fully supported by the Croatian Science Foundation
under the project ‘Genetic background of Dalmatian pyrethrum (Tanacetum cinerariifolium /
Trevir./ Sch. Bip.) insecticidal potential’ - (PyrDiv) (IP-06-2016-9034).

EVALUATION OF GLYCYRRHIZA L. GENETIC

RESOURCES IN THE CZECH REPUBLIC
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of licorice (Glycyrrhiza / )DEDFHDH 5RRWV DQG VWRORQV DUH IUHTXHQWO\ XVHG LQ SKDUPDF\
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Glycyrrhiza / FRQVLVWV RI DERXW VSHFLHV DQG DPRQJ WKHP WKH IROORZLQJ VSHFLHV ZHUH
evaluated: Glycyrrhiza glabra/Glycyrrhiza echinata/*O\F\UUKL]DSDOOLGLÀRUD Maxim,
Glycyrrhiza macedonica%RLV(W2USKGlycyrrhiza foetida'HVIDQGGlycyrrhiza uralensis
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PHENOLIC PROFILE, ANTIOXIDANT,

ACETYLCHOLINESTERASE AND TYROSINASE
ENZYME INHIBITORY ACTIVITIES OF ENDEMIC
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$QWRDQHWD7UHQGD¿ORYD1, Nikolina Kutova1, Gulmira Ozek2, Milka
Todorova1, Suleyman Yur2,3, Temel Ozek2,3

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CHITOSAN-PECTINE MICROPARTICLES LOADED

WITH SAGE EXTRACT FOR EXTENDING SHELL-LIFE
OF SAUSAGES
,YDQD&YHWNRYLNM.DUDQ¿ORYD1, Gjoshe Stefkov1, Marija
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Carcarevska2, Ljubica Cambuleva2, Dushko Shalabalija2, Vesna
Kotevska1, Ana Kaftandzieva3, Svetlana Kulevanova1

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high polyphenolic content and volatile compounds with well-established antimicrobial and
antioxidant potency directly to be added to food to improve its taste, smell and other organoleptic
properties, to increase the freshness of products and at least to preserve the products in a
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(innovative formulations and technological processes) to the aim of avoiding undesirable
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TEM images showed that the particles were with spherical morphology and relatively smooth
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E\WZRPHWKRGVȕFDURWHQHOLQROHLFDFLGDQGWKLREDUELWXULFDFLGUHDFWLYHVXEVWDQFHVDVVD\
CTP-SE showed higher antioxidant capacity compared to ascorbic acid (reference standard
VXEVWDQFHEXWUDWKHUPRGHUDWHDFWLYLW\FRPSDUHGWREXW\ODWHGK\GUR[\DQLVROH
Key words: 6DOYLDRI¿FLQDOLV/IRRGSUHVHUYDWLYHELRGHJUDGDEOHSDFNLQJ

NUTRACEUTICALS BASED ON BIOACTIVE

PRINCIPLES OF PLANT ORIGIN
Anca Boldea1, Radu Necula2,3, Ruxandra Mihaela Cretu2, Georgiana
Luminita Gavril2, Elvira Gille2

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2
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3
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,DVL5RPDQLD
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5HJLRQ 5RPDQLD RUJDQL]HG LQ SKDUPDFLHV SURYLGHV TXDOLW\ SKDUPDFHXWLFDO VHUYLFHV WR
SDWLHQWVDQGFXVWRPHUVE\WKHFRQWLQXRXVUHVHDUFKDFWLYLW\RI0DQDJHU3KDUP$QFD%ROGHD
3K' LQ 3KDUPDF\ LV IRFXVHG RQ IRUPXODWLQJ QHZ SURGXFWV IRU LPSURYLQJ OLIH TXDOLW\
1HZ IRUPXODWLRQV IRU LQWHUQDO JUDQXOHV FDSVXOHV V\UXS DQG H[WHUQDO VWLFN HVVHQWLDO RLO
XVHZHUHH[FOXVLYHO\SUHSDUHGE\$QFD3KDUPDF\7KHVHDUHEDVHGRQGLIIHUHQWPL[WXUHVRI
VWDQGDUGL]HG HVVHQWLDO RLOV HDFK SXUFKDVHG IURP PDQXIDFWXUHUV HJ WHD WUHH Eucalyptus
radiata$&XQQH['&Commiphora myrrha1HHV(QJOThymus vulgaris/FWWKXMDQRO
Cinnamomum camphora / - 3UHVO Hyssopus VS Satureja montana / Calophyllum
inophyllum /Lavandula spica/Nigella sativa/Abies alba0LOO6DOYLDRI¿FLQDOLV/
Citrus, Laurus nobilis/0HODOHXFDYLULGLÀRUD6ROH[*DHUWQOriganum compactum Benth,
RosmarinusFWYHUEHQRQH6RPHIRUPXODWLRQVDOVRFRQWDLQGLIIHUHQWK\GURDOFRKROLFH[WUDFWV
from Echinacea, Astragalus, Sambucus'XHWRWKHLUFRPSRVLWLRQWKHVHIRUPXODWLRQVFODLPWR
be used in phytotherapy to strengthen the immune system (immune-modulators and immune-
VWLPXODQWV LQ FRPPRQ FROG DQG ÀX UHVSLUDWRU\ LQIHFWLRQV FDXVHG E\ GLIIHUHQW SDWKRJHQV
UKLQR SKDU\QJLWLV EURQFKLWLV VDOSLQJLWLV SQHXPRQLD RWLWLV HWF DV QDWXUDO DQWLELRWLFV DQG
GLVLQIHFWDQWDQWLVHSWLFDQWLEDFWHULDODQWLIXQJLFDQWLYLUDODQWLLQÀDPPDWRU\DQWLWXVVLYHDQWL
FDWDUUKDOPXFRO\WLFDQGDQWLS\UHWLFDJHQWV7KHVHIRUPXODWLRQVZHUHDQDO\VHGE\³6WHMDUXO´
%5&XVLQJ*&06WRHYDOXDWHWKHYRODWLOHIUDFWLRQVLWZDVSHUIRUPHGZLWKDQ$JLOHQW1
JDV FKURPDWRJUDSK\ LQVWUXPHQW FRXSOHG WR DQ$JLOHQW PDVV VSHFWURPHWHU$ FDSLOODU\
FROXPQPîPPLGP'%ZDVXVHGIRUVHSDUDWLRQ7KHRWKHU*&±06
conditions (inlet mode, injection temperature, separation temperature program, scan range)
ZHUHRSWLPL]HG7KHVSHFWURPHWHUZDVRSHUDWHGLQHOHFWURQLPSDFW(,PRGHWKHTXDGUXSROH
DQGLRQL]DWLRQVRXUFHWHPSHUDWXUHZHUHDQG&UHVSHFWLYHO\7KHQHZIRUPXODWLRQV
ZLWKVWDQGDUGL]HGHVVHQWLDORLOVDUHFKDUDFWHUL]HGE\WKHGRPLQDQFHRIYRODWLOHIUDFWLRQVZLWK
VLJQL¿FDQWSKDUPDFRORJLFDODFWLYLWLHV
Key words: nutraceuticals, immunity, essential oils, gas-chromatography, phytotherapy

CARROT JUICE AS FUNCTIONAL FOOD OF PLANT

ORIGIN
0DUNR3HWHN%RULV/D]DUHYLü7RPLVODY.DUDåLMD0LKDHODâDWYDU,ULV
9XOHWLü0LUMDQD+HUDNûXVWLü
University of Zagreb, Faculty of Agriculture, Department of Plant Nutrition,

6YHWRãLPXQVNDFHVWD=DJUHE
&DUURWLVLPSRUWDQWYHJHWDEOHFURSWKDWLVJURZQIRUURRWVWKDWFDQEHGLIIHUHQWO\FRORXUHG,W
EHORQJVWRJURXSRIIXQFWLRQDOIRRGVWXIIVRISODQWRULJLQ,WLVULFKLQPLQHUDOVYLWDPLQVDQG
RWKHUSK\WRQXWULHQWVDQGKDVEHQH¿FLDOHIIHFWRQKXPDQKHDOWK&DUURWLVMXLF\YHJHWDEOHDQGLW
LVRIWHQXVHDVDSDUWRIGLIIHUHQWMXLFHPL[WXUHVDQGEDE\IRRG6XJDUFRQWHQWDQGS+RIMXLFH
SOD\LPSRUWDQWUROHLQWDVWHRIMXLFHDVZHOODVLQDONDOL]DWLRQRIWKHKXPDQERG\$OWKRXJKWKH
VXJDUFRQWHQWLQFDUURWLVKLJKHUWKDQWKHPRVWRWKHUYHJHWDEOHVLWKDVORZFDORULFYDOXH7KH
DLPRIWKLVVWXG\ZDVWRGHWHUPLQHDQGFRPSDUHWKHS+DQGVXJDUFRQWHQWLQMXLFHRIRUDQJH
FRORXUHGFDUURWURRWV6DPSOLQJZDVSHUIRUPHGLQDWULSOLFDWHLQWKHFLW\RI=DJUHELQWKUHH
VHOOLQJFKDQQHOVVDPSOHVLQWUDGHFKDLQVVDPSOHVLQPDUNHWVDQGVDPSOHVLQVWRUHVRI
HFRORJLFDOSURGXFWV7KHDYHUDJHS+YDOXHRIFDUURWMXLFHDFFRUGLQJWRVHOOLQJFKDQQHOUDQJHG
IURPWR6WDWLVWLFDOO\WKHKLJKHVWVXJDUFRQWHQWZDVGHWHUPLQHGLQFDUURWMXLFHIURP
VWRUHVRIHFRORJLFDOSURGXFWV%UL[
Key words:%UL[MXLFHS+VXJDUYHJHWDEOH

IN VITRO ASSESSMENT OF TWO SPECIES OF THE

GENUS PINUS GROWING IN ALGERIA FOR THEIR
ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY
Nadia Fekih1, Hocine Allali1, Salima Merghache1, Salim Bouchentouf1,
Jean Costa2

/DERUDWRLUHGHV6XEVWDQFHV1DWXUHOOHV %LRDFWLYHV/$61$%,2'pSDUWHPHQWGH&KLPLH
)DFXOWpGHV6FLHQFHV8QLYHUVLWp$ERX%HNU%HONDwG%37OHPFHQ$OJpULH
2
/DERUDWRLUH&KLPLHGHV3URGXLWV1DWXUHOV8QLYHUVLWpGH&RUVH805&156&DPSXV
*ULPDOGL%3&RUWH)UDQFH
A comparative study of volatile compounds from Pinus pinea / DQG Pinus pinaster Aiton
obtained by hydrodistillation extraction by GC and gas chromatography mass spectroscopy
*&06 ZDV FRQGXFWHG 7KH FROOHFWLYH HVVHQWLDO RLO RI P. pinea ZDV ULFK LQ OLPRQHQH
IROORZHG E\ ĮSLQHQH DQG ȕSKHOODQGUHQH 7KH PDMRU FRPSRXQGV IURP
the collective essential oil of P. pinaster were: ĮSLQHQHȕSLQHQHDQG(
ȕFDU\RSK\OOHQH7KHUHVXOWVVKRZHGWKDWWKHRLOVKDYHDJUHDWSRWHQWLDODVDQWLELRWLFV
against some microorganisms: Klebsiella pneumoniae, Enterococcus faecalis and Candida
albicans7KHPD[LPXP]RQHRILQKLELWLRQRIP. pinea was obtained against Candida albicans (23
PP$QWLR[LGDQWFDSDFLW\ZDVDVVHVVHGE\in vitro WHVWLQJXVLQJGLSKHQ\OSLFU\OK\GUD]\O
'33+DVVD\DQGVLJQL¿FDQWDFWLYLW\ZDVIRXQGIRUWKHYDULRXVRLOV7KHVHUHVXOWVVXJJHVWHG
that essential oils of P. pinea and P. pinaster processes antimicrobial and antioxidant properties
DQGDUHWKHUHIRUHDSRWHQWLDOVRXUFHRIDFWLYHLQJUHGLHQWVIRUIRRGDQGSKDUPDFHXWLFDOLQGXVWU\
Key words: Pinaceae, essential oils, GC, GCMS, biological activities

INFLUENCE OF HARVEST TIME AND GROWING

LOCATION ON THE PHENOLICS AND ANTIOXIDANT
ACTIVITY OF MASTIC TREE LEAVES
,YRQD(OH]*DURIXOLü16DQGUD3HGLVLü16DQMD'UDJRYLü2, Mateja
Cestar19DOHULMD)DELMDQLü1-DVQDäLEHUW19HULFD'UDJRYLü8]HODF1

8QLYHUVLW\RI=DJUHE)DFXOW\RI)RRG7HFKQRORJ\DQG%LRWHFKQRORJ\3LHURWWLMHYD
Zagreb, Croatia
2
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The objective of this study was to evaluate the effect of growing location and harvest time on
WRWDOSKHQROLFV73ÀDYRQRLGV7)K\GUR[\FLQQDPLFDFLGV7+&$DQGÀDYRQROV7)ODY
of mastic tree (Pistacia lentiscus /LQQ OHDYHV DV ZHOO DV RQ LWV DQWLR[LGDQW DFWLYLW\ $$
/HDYHVZHUHVDPSOHGDWIRXUGLIIHUHQWJURZLQJORFDWLRQV%DUEDULJD/XQ+YDU.RUþXODDW
WKUHHKDUYHVWSHULRGV0D\$XJXVW2FWREHU4XDQWLWDWLYHGHWHUPLQDWLRQVRISKHQROLFV
were conducted spectrophotometrically and AA by ferric reducing antioxidant power method
)5$3 2XU ¿QGLQJV VXJJHVW WKDW SKHQROLF FRQWHQW DQG $$ YDULHG DFFRUGLQJ WR JURZLQJ
ORFDWLRQDQGKDUYHVWWLPH7KHPHDQ73FRQWHQWUDQJHGIURPWRPJJ, TF from
WRPJJ7+&$IURPWRPJJDQG7)ODYIURPWRPJJ7KH
KLJKHVW737+&$DQG7)ODYFRQWHQWZDVGHWHUPLQHGLQ0DVWLFWUHHOHDYHVKDUYHVWHGLQ0D\DW
.RUþXODEXWDOVRDOPRVWDOODQDO\VHGSDUDPHWHUVZHUHWKHORZHVWLQOHDYHVKDUYHVWHGLQ$XJXVW
DWVDPHJURZLQJORFDWLRQ7KHVDPHWUHQGZDVREVHUYHGLQVDPSOHVIURP/XQDQG%DUEDULJD
EXWKDUYHVWHGLQ$XJXVWDQG2FWREHULQGLFDWLQJGHFUHDVHLQSKHQROLFFRQWHQWDQGDQWLR[LGDQW
DFWLYLW\RIPDVWLFWUHHOHDYHVDWODWHUKDUYHVWLQJSHULRGV$129$VKRZHGDVLJQL¿FDQWHIIHFW
RI JURZLQJ ORFDWLRQ RQ DOO SKHQROLFV ZKLOH KDUYHVW WLPH DIIHFWHG PRUH7) FRQWHQW DQG$$
7KHKLJKHVWPJJDQGWKHORZHVWPJJ) AA was determined in leaves from
%DUEDULJD,VWULDKDUYHVWHGLQ$XJXVWDQG2FWREHUUHVSHFWLYHO\
Key words: antioxidant activity, harvest time, growing location, mastic tree leaf, phenolics

ANTIOXIDATIVE POTENTIAL OF MACEDONIAN PINE

0DULMD.DUDSDQG]RYD,YDQD&YHWNRYLNM.DUDQ¿ORYD*MRVKH6WHINRY
Svetlana Kulevanova
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7KHLPSRUWDQFHRIR[LGDWLRQLQWKHERG\KDVEHHQZLGHO\UHFRJQL]HGDVR[LGDWLYHPHWDEROLVP
LVHVVHQWLDOIRUWKHVXUYLYDORIFHOOV$VLGHHIIHFWRIWKLVGHSHQGHQFHLVWKHSURGXFWLRQRIIUHH
radicals and other reactive oxygen species that cause oxidative changes as well as destructive
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WKXVVKXWWLQJGRZQWKHFHOOXODUUHVSLUDWLRQ7KHRQO\GHIHQVHPHFKDQLVPDJDLQVWWKHHIIHFWV
RIH[FHVVLYHR[LGDWLRQVLVSURYLGHGE\WKHDFWLRQRIYDULRXVDQWLR[LGDQWV$OWKRXJKLWLVZHOO
NQRZQ WKDW VRPH RI SLQH VSHFLHV DUH FKDUDFWHUL]HG E\ VLJQL¿FDQW DQWLR[LGDQW DFWLYLW\ YHU\
OLWWOH LV NQRZQ DERXW WKH DQWLR[LGDWLYH SRWHQWLDO RI HQGHPLF 0DFHGRQLDQ SLQH 03 Pinus
peuce*ULVHE3LQDFHDH,QWKLVRUGHUDQWLR[LGDWLYHDFWLYLW\RI03¶VHVVHQWLDORLODQGPHWKDQRO
H[WUDFWVRIQHHGOHVDQGEDUNKDVEHHQLQYHVWLJDWHGE\'33+7%$56DQG)5$3DVVD\0HWKDQRO
H[WUDFWVRI03¶VQHHGOHVKDYHVKRZHGFRQVLGHUDEOHUDGLFDOVFDYHQJLQJDFWLYLW\LQ'33+DVVD\
DV WKH SHUFHQWDJH RI LQKLELWLRQ DQG ,& YDOXHV UDQJHG IURP DQG IURP
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PL-1IRU7%$56DQGIURPDQGPJ mL-1IRU)5$3DVVD\UHVSHFWLYH
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Key words: Pinus peuceHVVHQWLDORLOH[WUDFWEDUNQHHGOHV

PELARGONIUM RADENS H. E. Moore:

PHYTOCHEMICAL PROFILE AND IN VITRO
ANTIOXIDANT ACTIVITY
Cristina Iancu1, Cornelia Mircea1, Oana Cioanca2$QGUHHD0XUăUD‫܈‬X1,
Nina Filip3, Madalina Mocanu3, Monica Hancianu2

³*ULJRUH73RSD´8QLYHUVLW\RI0HGLFLQHDQG3KDUPDF\)DFXOW\RI3KDUPDF\'HSDUWPHQW
RI3KDUPDFHXWLFDO%LRFKHPLVWU\DQG&OLQLFDO/DERUDWRU\8QLYHUVLWDWLL6WUHHW
Iasi, Romania
2
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RI3KDUPDFRJQRV\8QLYHUVLWDWLL6WUHHW,DVL5RPDQLD
3
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8QLYHUVLWDWLL6WUHHW,DVL5RPDQLD
Pelargonium radens + ( 0RRUH LV D VSHFLHV WKDW EHORQJV WR 3HODUJRQLXP IDPLO\ DQG
UHSUHVHQWVDWD[RQRPLFLPSRUWDQFHIRULWVFODVV1RZDGD\VLWVH[WUDFWVKDYHJUHDWLPSRUWDQFH
IRU SHUIXPH LQGXVWU\ 7KH PDFURVFRSLFDO DQG RUJDQROHSWLF DQDO\VLV LQGLFDWHG WKDW FUXVKLQJ
the leaves allows a stronger citrus fragrance to release, probably due to glandular hairs
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ZDVREWDLQHGE\8+3/&WHFKQLTXH7KHFKHPLFDOSUR¿OHLQFOXGHGUXWRVLGHJPJ
FLQDURVLGHJPJFYHUFHWRODUDELQRVLGHJPJDQGNDHPSIHUROJ
PJDOVRFDWHFKLQJPJDQGHSLFDWHFKLQJPJDQGSROLSKHQROLFGHULYDWLYHV
PDLQO\FDIHLFDFLGJPJ7KHELRORJLFDOSUR¿OHRIWKHLQYHVWLJDWHGH[WUDFWVKRZHGD
JRRGDQWLR[LGDQWDFWLYLW\DJDLQVWWKH'33+DQG$%76UDGLFDOV0RUHRYHUDVWURQJVFDYDQJHU
SRWHQWLDO ZDV QRWHG DJDLQVW VXSHUR[LGH DQLRQ )RU WKH SURSHU DVVHVVPHQW RI WKH DQWLR[LGDQW
SRWHQWLDOZHFDOFXODWHGWKHHI¿FLHQWFRQFHQWUDWLRQIRUHDFKDVVD\7KHUHVXOWVLQGLFDWHG
WKDW WKH H[WUDFW ZDV PRVW DFWLYH DJDLQVW$%76 J PL-1 IROORZHG E\ '33+
J PL-1 DQG VXSHUR[LGH DQLRQ J PL-1 7KH FRQFHQWUDWLRQ
RIWKHDFWLYHcompounds and their reducing character has a great impact in the actual antioxi-
dant activity of SODQW H[WUDFWV 0RVW FRPPRQO\ SODQWV FUHDWH VXFK VHFRQGDU\ PHWDEROLWHV WR
DGDSWDQGVXUYLYHWKHHQYLURQPHQWDOFKDQJLQJFRQGLWLRQV:HFDQRQO\EHQH¿WDVKXPDQVE\
XVLQJKLJKTXDOLW\SODQWPDWHULDO
Key words: Pelargonium, antioxidant, poliphenols

BUNIAS ERUCAGO L. : CHEMISTRY AND BIOLOGICAL

ACTIVITY
$]UDĈXORYLü1, Andrea Sedlar19HGUDQDýLNHãýXOLü4,YDQD*HQHUDOLü
0HNLQLü2)UDQNR%XUþXO3,YLFD%ODåHYLü1

8QLYHUVLW\RI6SOLW)DFXOW\RI&KHPLVWU\DQG7HFKQRORJ\'HSDUWPHQWRI2UJDQLF
&KHPLVWU\5XÿHUD%RãNRYLüD6SOLW
2
University of Split, Faculty of Chemistry and Technology, Department of Food Technology
DQG%LRWHFKQRORJ\5XÿHUD%RãNRYLüD6SOLW
3
University of Split, Faculty of Chemistry and Technology, Department of Analytical
&KHPLVWU\5XÿHUD%RãNRYLüD6SOLW

6FKRRORI0HGLFLQHâROWDQVND6SOLW
9RODWLOH VXOIXU FRPSRXQGV DUH IRXQG LQ PDQ\ VSHFLHV RI SODQW IDPLOLHV DQG SODQW
JHQHUDLQFOXGLQJWKHPRVWLPSRUWDQWIDPLO\%UDVVLFDFHDH*OXFRVLQRODWHVȕWKLRJOXFRVLGH
1K\GUR[\VXOIDWHVZLWKYDULDEOHVLGHFKDLQVDUHVHFRQGDU\PHWDEROLWHVLGHQWL¿HGLQRYHU
GLIIHUHQWSODQWIDPLOLHV7KHVHFRPSRXQGVDUHIRXQGSDUWLFXODUO\LQWKH%UDVVLFDFHDHIDPLO\
Bunias erucago/&RUQ5RFNHW&UHVWHGZDUW\FDEEDJHLVZLOGJURZLQJSODQWLQ6RXWKHUQ
(XURSH/HDYHV\RXQJVWHPVDVZHOODVURRWVDUHHGLEOHSDUWVHDWHQUDZRUFRRNHG³3D]LMD´
D7XUNLVKQDPHIRUEHHW%HWDRU³SDNROHü´LVWUDGLWLRQDOIRRGSUHSDUHGDVDPL[WXUHRIZLOG
vegetables boiled in a mixture where the dominant species is B. erucagoSamples of B. erucago
ZHUHFROOHFWHGIURPWZRGLIIHUHQWORFDWLRQV0DUMDQKLOO6SOLWDQGLVODQGRI%UDþâNULS7KH
JOXFRVLQRODWHLGHQWL¿FDWLRQZDVFDUULHGRXWLQGLUHFWO\E\WKHLUGHJUDGDWLRQYRODWLOHSURGXFWV
were obtained by hydrodistillation and organic solvent extraction, mostly isothiocyanates and
QLWULOHVXVLQJ*&06WHFKQLTXH$QDO\VLVRIB. erucagoIURPLVODQGRI%UDþKDVVKRZQWKH
presence of glucotropaeoline and glucodehydroerucin while in the plant collected on Marjan
KLOO ¿YH W\SHV RI JOXFRVLQRODWHV KDYH EHHQ LGHQWL¿HG JOXFRQDSLQ JOXFRWURSDHROLQ KHSW\O
JOXFRVLQRODWH JOXFRGHK\GURHUXFLQ DQG JOXFRHUXFLQ9RODWLOH VXOIXU LVRODWHV ZHUH DOVR WHVWHG
IRUWKHLUDQWLR[LGDWLYHDQWLFDQFHUDQGFKROLQHVWHUDVHLQKLELWRU\DFWLYLWLHV
Key words: Bunias erucago/JOXFRVLQRODWHV*&06ELRORJLFDODFWLYLW\

III. CULTIVATION, BREEDING AND BIOTECHNOLOGY

OF MEDICINAL AND AROMATIC PLANTS

Book of abstracts – 10th CMAPSEEC PosterPleanry lecture
presentations
METABOLOMICS, PLANT BIOTECHNOLOGY AND

LEAD FINDING: PERFECT HOLISTIC MATCH?
Milen I. Georgiev1,2, Andrey Marchev1,2, Ivanka Koycheva1,2, Kristiana
Amirova2, Liliya Vasileva2

7KH6WHSKDQ$QJHORII,QVWLWXWHRI0LFURELRORJ\%XOJDULDQ$FDGHP\RI6FLHQFHV5XVNL
%OYG3ORYGLY%XOJDULD
2
Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria
3HU GH¿QLWLRQ PHWDERORPLFV UHSUHVHQWV D FRPSUHKHQVLYH KROLVWLF DSSURDFK FRPSULVLQJ

RI V\VWHPDWLF LGHQWL¿FDWLRQ DQG TXDQWL¿FDWLRQ RI DOO PHWDEROLWHV LQ DQ RUJDQLVP DW JLYHQ
FRQGLWLRQV 7KH FRPSUHKHQVLYH DQDO\VLV RI WKH FKHPLFDO ¿QJHUSULQWV OHIW E\ PHWDEROLF
SURFHVVHV VWDUWHG WR SOD\ D FUXFLDO UROH LQ WKH SHUVRQDOL]HG PHGLFLQH 6LQFH WKH ULVH RI WKH
omics age several platforms for high throughput analyses of targeted metabolites have been
GHYHORSHG1XFOHDUPDJQHWLFUHVRQDQFH105DSSHDUVYHU\VXLWDEOHDQGDGHTXDWHSODWIRUP
to carry out metabolomics analyses, as it allows simultaneous detection of diverse range of
abundant (primary and secondary) metabolites, which opens novel avenues to fully explore the
WRWDOELRFKHPLFDOPDFKLQHU\RISODQWV$JUHDWDGYDQWDJHRI+105VSHFWURPHWU\RYHUWKH
RWKHUDQDO\WLFDOSODWIRUPVLVWKHSRVVLELOLW\IRUTXDQWL¿FDWLRQDQGKHQFHWKHGLUHFWFRPSDULVRQ
RIFRQFHQWUDWLRQVRIDOOFRPSRXQGVSUHVHQWLQWKHVDPSOH6RPHFDVHVWXGLHVIURPDXWKRU¶V
laboratory, on the application of NMR-based metabolomics concept in natural products
UHVHDUFKSODQWELRWHFKQRORJ\DQGOHDG¿QGLQJZLOOEHWKRURXJKO\SUHVHQWHGDQGGLVFXVVHG
Key words:105QDWXUDOSURGXFWVVXVWDLQDELOLW\OHDG¿QGLQJ
$FNQRZOHGJHPHQW$XWKRUVDFNQRZOHGJHWKH¿QDQFLDOVXSSRUWRIWKH(XURSHDQ8QLRQ¶V
Horizon 2020 research and innovation programme, project PlantaSYST (SGA-CSA No.
XQGHU)3$1R

CHOOSING THE RIGHT APPROACH FOR

CONDUCTING MEDICINAL PLANTS CULTIVATION
TRIAL
'HMDQ3OMHYOMDNXãLü
,QVWLWXWHIRU0HGLFLQDO3ODQWV5HVHDUFKÄ'U-RVLI3DQþLü³
7DGHXãD.RãüXãND%HOJUDGH6HUELD
Pioneer attempts in the cultivation of wild plants as well as improving the genetic base or
agro-technology, can often be unsuccessful or misleading unless researchers consider some of
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consumption in weeding and harvesting could limit the number of plants and dimensions of the
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Different approaches could be applied in dealing with outliers, population distributions and
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EFFECT OF IRRIGATION ON THE PRODUCTION AND

SECONDARY METABOLITES OF SUMMER SAVORY
(SATUREJA HORTENSIS L. ‘BUDAKALÁSZI’)
3pWHU5DGiFVL1, Katalin Inotai23pWHU5DMKiUW1, Szilvia Sárosi1, Éva
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Key words: savory, stress, drought, water supply, irrigation

IMPACT OF NITROGEN FERTILIZATION AND SOWING

NORMS ON CHLOROPHYLL FLUORESCENCE OF
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photosynthesis play a critical role in understanding the reactions of plants to environmental
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associated with the exposure of plants to stress, indicating that most of the captured light is not
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THE SUCCESSFUL BREEDING WORK OF MEDICINAL

PLANTS IN SLOVAKIA
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mint (Menthaîpiperita/DQGODYHQGHULavandula angustifolia/DUHWKHPRVWFXOWLYDWHG
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countries in which particular attention has been devoted to the research of medicinal, aromatic
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Key words: new variety, chamomile, mint

DRYING PERFORMANCE OF AMARANTH LEAVES

(AMARANTHUS spp.) USING AN INFLATABLE SOLAR
DRYER
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leaves has become a challenge for processors due to its exposure to contamination and high
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Key words: solar drying, leaf drying, solar bubble dryer, plant

ASSESSMENT OF THE PROFILE AND ACCUMULATION

DYNAMICS OF SECONDARY METABOLITES IN
FERTILIZED ORNAMENTAL ASTERACEAE
Oana Cioanca1, Cristina Cerasela Mircea (Arsene)1, Ana Flavia Burlec1,
Adrian Spac1, Andreea Corciova1, Elvira Gille2, Silvia Robu3, Monica
Hancianu1

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The ornamental species represent an interesting potential source of secondary metabolites

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Chrisanthemum indicum / Rudbeckia hirta / Echinacea purpurea / 0RHQFK
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TWO NEW CHEMOTYPES OF BALM (MELISSA

OFFICINALIS L.)
Johannes Kittler1+DQV.UJHU2, Ute Kästner1, Ulrike Lohwasser3,
Detlef Ulrich2, Bärbel Zeiger2&KULVWRSK%|WWFKHU2, Andrea Krähmer2,
Gennadi Gudi2, Heidi Heuberger4, Anna Artemyeva5, Olga Zvereva5,
Daniel Becker1, Frank Marthe1

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proven sedative, spasmolytic and antiviral effects, it is often used in watery or alcoholic
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gas chromatography (GC) was conducted, as well as extraction as method adapted for small
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INITIATION OF IN VITRO CLONES OF TANACETUM

CINERARIIFOLIUM Trevir. Sch. Bip. (ASTERACEAE)
Vladimir Ilinkin, Marina Stanilova
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences 23,

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Dalmatian pyrethrum (Tanacetum cinerariifolium 7UHYLU 6FK %LS Chrysanthemum

cinerariefolium) is a perennial herbaceous plant belonging to the Asteraceae family, endemic to
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DUHOHDGLQJLQVHFWLFLGHVLQRUJDQLFIDUPLQJ7KHDLPRIWKHSUHVHQWVWXG\ZDVWRLQLWLDWHin vitro
clones of T. cinerariifoliumDVD¿UVWVWHSIRUVHOHFWLRQRIKLJKSURGXFWLYHS\UHWKULQFORQHV
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from ex situFROOHFWLRQRIS\UHWKUXPLQ%XOJDULDZHUHXVHGDVDVWDUWLQJPDWHULDO$IWHUVWDQGDUG
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formed at the base of the explants were separated, and the longer ones were additionally cut
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Key words: Dalmatian pyrethrum, pyrethrins, bioinsecticides

GROWTH AND ESSENTIAL OILS OF SALVIA

OFFICINALIS L. PLANTS DERIVED FROM
CONVENTIONAL OR AEROPONIC PRODUCED
SEEDLINGS
Boryanka Traykova, Marina Stanilova, Milena Nikolova, Strahil
Berkov
6DOYLD RI¿FLQDOLV / /DPLDFHDH LV FXOWLYDWHG LQ PDQ\ FRXQWULHV DV D YDOXDEOH PHGLFLQDO
and aromatic plant with antiseptic and spasmolytic effects due to its essential oils, used as
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seedlings production, used as a control, began in November (in terrines with ordinary soil)
while that on aeroponic vertical system began the next year in April (seeds sown into peat cubes
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the application of aeroponic system shortened the period from germination to harvest for 5
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Key words: sage, medicinal and aromatic plant, hydroponic technologies, cultivation

ROOTING OF LEAVES OF HABERLEA RHODOPENSIS

Friv. (GESNERIACEAE) USING A VERTICAL
AEROPONIC SYSTEM
Boryanka Traykova, Marina Stanilova
Haberlea rhodopensis)ULYLVDWHUWLDU\UHOLFWDQGHQGHPLFVSHFLHVWRWKH%DONDQ3HQLQVXOD
GLVWULEXWHG RQO\ LQ %XOJDULD DQG *UHHFH 7KLV SHUHQQLDO KHUEDFHRXV SODQW LV JURZLQJ LQ
VKDG\ URFN FUHYLFHV RQ OLPHVWRQH DW DOWLWXGHV RI ± P ,W EHORQJV WR WKH VR FDOOHG
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FRPSOHWHZDWHUORVVDQGWRUHFRYHUIDVWXSRQUHZDWHULQJ3ODQWVZHUHXVHGLQWKHSDVWIRULWV
KHDOLQJDQGUHMXYHQDWLQJSURSHUWLHV7KHVSHFLHVLVSURWHFWHGE\WKH%LRGLYHUVLW\$FWDQGWKH
$FWRI0HGLFLQDO3ODQWV7KHDLPRIWKHVWXG\ZDVWRVWLPXODWHWKHURRWLQJRIH. rhodopensis
leaves by applying hydroponic technologies, testing different substrates and IBA treatment,
DVD¿UVWVWHSWRZDUGSODQWSURSDJDWLRQ/HDYHVIURPRQH%XOJDULDQORFDOLW\ZHUHXVHGDVD
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PLQHUDOZRRODQGSHUOLWHLQDVHPLSHUPHDEOHWLVVXHVXUURXQGHGHLWKHUE\NHUDP]LWHRUDJURODYD
SHEEOHVOHDYHVSHUYDULDQW+DOIRIWKHOHDYHVLQHDFKYDULDQWZHUHWUHDWHGZLWK,%$SRZGHU
SULRUWROHDIHPEHGGLQJLWWKHVXEVWUDWH%HVWUHVXOWVZHUHREWDLQHGZLWK,%$WUHDWHGOHDYHV
RQSHUOLWHVXUURXQGHGZLWKDJURODYDZKHUHRIWKHOHDYHVGHYHORSHGYLJRXUURRWV\VWHP
IRUZHHNV%RWKVXEVWUDWHVDQG,%$LQÀXHQFHGWKHURRWLQJSURFHVV7KHHIIHFWRI,%$ZDV
SRVLWLYHH[FHSWIRUWKHYDULDQWZLWKNHUDP]LWHSUREDEO\GXHWRLWVSURSHUW\WRGHFUHDVHS+LQ
WKHSRWV7KHORZHVWSHUFHQWRIURRWHGOHDYHVZDVREWDLQHGZLWKSHDWFXEHVZLWKRXW,%$
WUHDWPHQW,QWKHYDULDQWZLWKPLQHUDOZRROLQWHUPHGLDWHVXFFHVVZDVREVHUYHG6LQJOHURVHWWHV
of H. rhodopensisEHJDQWRDSSHDUDIWHUPRQWKVRIWKHOHDIFXOWLYDWLRQ
Key words: endemics, relicts, resurrection plants, hydroponic cultures, medicinal plants

IN VITRO ESTABLISHMENT AND CULTURE

OF ENDANGERED MEDICINAL PLANTS WITH
ORNAMENTAL VALUE CAMPTOTHECA ACUMINATA
Decne (NYSSACEAE)
Lilyana Nacheva1, Nataliya Dimitrova1, Valeria Ivanova2

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2
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Camptotheca acuminata Decne (happy tree, cancer tree, or tree of life) is a deciduous
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PRQRWHUSHQHLQGROHDONDORLGLVRODWHGIURPWKHEDUNDQGVWHPVRIC. acuminata&DPSWRWKHFLQ
and its derivatives are used for the treatment different cancers and have potential for treatment
RIDZLGHUDQJHRIYLUXVHVDQGSDUDVLWHV7KHDLPRIWKHSUHVHQWVWXG\ZDVWRGHYHORSPHWKRGV
for in vitroPLFURSURSDJDWLRQRIWKLVYDOXDEOHSODQW'LIIHUHQWFXOWXUDOPHGLDEDVHGRQERWK
060XUDVKLJHDQG6NRRJRU'.:'ULYHUDQG.XQL\XNLIRUPXODWLRQVZLWK
GLIIHUHQW F\WRNLQLQV %$3 RU LVRSHQWHQ\ODGHQLQH RU PHWDWRSROLQ KDYH EHHQ LQYROYHG LQ
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Key words: Camptotheca acuminata, camptothecin, micropropagation

ANTIOXIDANT ENZYMES AND OXIDATIVE STRESS

MARKERS IN HYPERICUM PERFORATUM L.
TRANSGENIC SHOOT CULTURES
Oliver Tusevski, Marija Krstikj, Ivona Rashkovska, Marija Runtevska,
Sonja Gadzovska Simic
'HSDUWPHQWRI3ODQW3K\VLRORJ\$UFKLPHGRYDVWU6NRSMH5HSXEOLFRI0DFHGRQLD
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DQGR[LGDWLYHVWUHVVPDUNHUFRQWHQWVLQHypericum perforatum/WUDQVJHQLFVKRRWV767KH
TS clones were regenerated from the corresponding hairy roots induced by Agrobacterium
rhizogenes $PHGLDWHGWUDQVIRUPDWLRQ,QDGGLWLRQFRQWUROURRWVZHUHXVHGIRUUHJHQHUDWLRQ
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GHPRQVWUDWHGWKDWWKHXSUHJXODWLRQRI3;DFWLYLW\QNDWāPJ proteins) in TS clones
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RI&$7DQG$3;LQ76FORQHVPD\EHFRPSHQVDWHGE\WKHDFWLYLW\RI3;WKDWDFWFRQFXUUHQWO\
WRUHPRYH+2227KH76FORQHVH[KLELWHGDQHQKDQFHPHQWLQ62'DFWLYLW\8 PJ
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WKHDFWLYDWLRQRIDQWLR[LGDQWGHIHQFHV\VWHPVLJQL¿FDQWO\FRQWULEXWHGWRWKHLPSURYHPHQWRI
oxidative stress tolerance in H. perforatumWUDQVJHQLFSODQWV
Key words:DQWLR[LGDQWHQ]\PHVHypericum perforatum/R[LGDWLYHVWUHVVPDUNHUV

transgenic shoots

SEASONAL VARIATION OF THE ESSENTIAL OIL AND

POLYPHENOL/FLAVONOID CONTENT OF LEAVES OF
SALVIA FRUTICOSA Mill. AND S. POMIFERA L. PLANTS
CULTIVATED IN THE BOTANICAL GARDEN OF
UNIVERSITY OF PATRAS
Giannis Anastasopoulos1, Peggy Leontaritou2, Vassilis
Papasotiropoulos3, Gregoris Iatrou2, Fotini N. Lamari1

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2
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3
'HSDUWPHQWRI$JULFXOWXUDO7HFKQRORJ\7(,RI:HVWHUQ*UHHFH$PDOLDGD*UHHFH
SalviaLVWKHODUJHVWJHQXVRIWKH/DPLDFHDHIDPLO\DQGLQFOXGHVDERXWWD[DVSDQGVVS
WKURXJKRXW WKH ZRUOG S. fruticosa 0LOO *UHHN VDJH DQG S. pomifera / &UHWDQ VDJH DUH
FRPPRQLQ*UHHFHDQGDUHXVHGLQWUDGLWLRQDOPHGLFLQHDQGFRRNLQJ7KHSXUSRVHRIWKLVVWXG\
was the analysis of seasonal variation of the volatile and non-volatile secondary metabolites of
S. fruticosa and S. pomiferaFORQHVFXOWLYDWHGLQWKHERWDQLFDOJDUGHQRI8QLYHUVLW\RI3DWUDV
These clones originate from plants collected at Messenia and Achaia regions (S. fruticosa),
and from Arcadia region (S. pomiferaQ DQGUHVSHFWLYHO\'ULHGOHDYHVFROOHFWHGLQ
$SULO-XO\'HFHPEHUDQG2FWREHUZHUHVXEMHFWHGWRVWHDPGLVWLOODWLRQIRUKDQGDQDO\VLV
ZDVSHUIRUPHGE\*&061RQYRODWLOHVZHUHH[WUDFWHGZLWKPHWKDQRODIWHUGHIDWWLQJ
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VHDVRQVWKHKLJKHUYDOXHVZHUHREVHUYHGGXULQJVSULQJWLPH6WDWLVWLFDOO\VLJQL¿FDQWGLIIHUHQFHV
RIWKHHVVHQWLDORLO\LHOGZHUHDOVRUHFRUGHGWKHKLJKHVW\LHOGZDVUHFRUGHGLQVXPPHUZKLOH
WKHORZHVWLQVSULQJ7KHPDLQS. fruticosaHVVHQWLDORLOFRPSRQHQWVZHUHHXFDO\SWRO
FDPSKRU DQG YLULGLÀRURO ZKHUHDV LQ S. pomifera oil were
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In both S. fruticosa populations, the levels of alpha-pinene were higher in summer, while in
VSULQJ FDU\RSK\OOHQH DQG LWV R[\JHQDWHG GHULYDWLYHV ZHUH VLJQL¿FDQWO\ HOHYDWHG 5HJDUGLQJ
S. pomiferaWKHORZHVWFRQWHQWLQWKXMRQHZDVREVHUYHGLQVSULQJ,QFRQFOXVLRQZHREVHUYHG
SRSXODWLRQDQGVHDVRQDOYDULDWLRQLQWKHFKHPLFDOSUR¿OHRIWKHHVVHQWLDORLOVRIWKHFORQHV
VWXGLHG7KLVZRUNFRQWULEXWHVWRGHOLQHDWLQJWKHPHWDEROLFPHFKDQLVPVDQGWRLGHQWLI\LQJWKH
SDUDPHWHUVFULWLFDOIRULVRODWLQJKLJKTXDOLW\Salvia HVVHQWLDORLOV
Key words: Salvia, cultivation, essential oil, polyphenols

SHOOT MULTIPLICATION SYSTEM OF HYSSOP

(HYSSOPUS OFFICINALIS L.)
Ely Zayova, Maria Geneva, Ludmila Dimitrova, Maria Petrova
$Q HI¿FLHQW PHWKRG IRU WKH PLFURSURSDJDWLRQ RI K\VVRS +\VVRSXV RI¿FLQDOLV / KDV EHHQ
GHYHORSHG7KHSODQWVZHUHFXOWXUHGRQ0XUDVKLJHDQG6NRRJPHGLXP06VXSSOHPHQWHG
ZLWKEHQ]\ODPLQRSXULQHWKLGLD]XURQ]HDWLQDQGLQGROHEXW\ULFDFLGGXULQJIRXUZHHNV
RIFXOWXUH7KHDERYHPHQWLRQHGF\WRNLQLQVZHUHDSSOLHGLQFRQFHQWUDWLRQDQGPJO
0D[LPXPPXOWLSOLFDWLRQIUHTXHQF\QXPEHUVRIVKRRWVVKRRWOHQJWKIUHVKDQGGU\ZHLJKWZHUH
HVWDEOLVKHGDWPJOEHQ]\ODPLQRSXULQHFRPELQHGZLWKPJOLQGROHEXW\ULFDFLG
7KHRWKHUWHVWHGF\WRNLQLQVWKLGLD]XURQDQG]HDWLQZHUHOHVVHIIHFWLYHIRUWKHPLFURSURSDJDWLRQ
RI K\VVRS )RU URRW LQGXFWLRQ WKH PLFURSURSDJDWHG SODQWV IURP WKH PRVW HIIHFWLYH YDULDQW
06VXSSOLHGZLWKPJOEHQ]\ODPLQRSXULQHDQGPJO indole-3-butyric acid) were
FXOWXUHGRQURRWGHYHORSPHQWPHGLXP7KHXQLIRUPPLFURVKRRWVZHUHH[FLVHGDQGWUDQVIHUUHG
to the rooting medium contained half strength MS medium supplemented with three types of
DX[LQVLQGROHEXW\ULFDFLGDQGLQGROHDFHWLFDFLGDSSOLHGDWDFRQFHQWUDWLRQRIPJO
ZLWKDGGLWLRQRIVXFURVH7KHREWDLQHGUHVXOWVVXJJHVWWKDWDOOWKUHHLQYHVWLJDWHGDX[LQV
could be used separately in ½ MS medium for rooting of +RI¿FLQDOLV,QGROHEXW\ULFDFLG
ZDVPRUHHIIHFWLYHWRSURGXFHSODQWVZLWKZHOOGHYHORSHGURRWVWKHUHIRUHWKLVDX[LQZDVIRXQG
WREHWKHEHVWURRWLQJKRUPRQHDVRSSRVHGWRĮQDSKWKDOHQHDFHWLFDFLGDQGLQGROHDFHWLF
DFLG7KHPXOWLSOHSODQWVZHUHVXFFHVVIXOO\ex vitroDGDSWHGZLWKVXUYLYDO7KHGHVFULEHG
protocol allows the establishment of numerous micropropagated plants of +RI¿FLQDOLV
Key words: +\VVRSXVRI¿FLQDOLV, micropropagation, plant growth regulators
3URMHFW'1

THE EFFECT OF IRRIGATION AND ORGANIC

FERTILIZATION ON THE ESSENTIAL OIL
COMPOSITION OF MATRICARIA CHAMOMILLA L.
Eleni Wogiatzi1, Eirini Sarrou2, Athina Logotheti1, Alexandros
Vrachoritis1, Kyriakos Gianoulis1, Paschalina Chatzopoulou2

Technological Educational Institute of Thessaly, Department of Agronomy Technology,
/DULVVD*UHHFH
2
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5HVRXUFHV'HSDUWPHQWRI$URPDWLFDQG0HGLFLQDO3ODQWV7KHUPL7KHVVDORQLNL
Greece
Chamomile (Matricaria recutita/IDP$VWHUDFHDHLVDQLPSRUWDQWPHGLFLQDOSODQWZLOG

JURZQDQGFXOWLYDWHGZRUOGZLGH7KHGUXJLVFRQVWLWXWHGIURPWKHGULHGÀRZHUKHDGVDQGLW
is used for the preparation of herbal teas, in phytotherapy, cosmetics, in veterinary medicine,
HWF &KDPRPLOH HVVHQWLDO RLO DQG H[WUDFWV FRQWDLQ PRUH WKDQ VHFRQGDU\ PHWDEROLWHV
ZKLFK FRQWULEXWH WR LWV SKDUPDFRORJLFDO SURSHUWLHV 6HVTXLWHUSHQHV ÀDYRQRLGV FRXPDULQV
DQGSRO\DFHW\OHQHVDUHWKHPRVWLPSRUWDQWJURXSRIFRQVWLWXHQWV&KDPRPLOH¶VEOXHHVVHQWLDO
RLO FRQWDLQV PDLQO\ VHVTXLWHUSHQH GHULYDWLYHV DQG DPRQJ WKHP Į ELVDERORO FKDPD]XOHQH
Į ELVDERORO R[LGHV$ DQG % DUH WKH PDLQ FRPSRXQGV &KDPD]XOHQH DQG Į ELVDERORO DUH
FRQVLGHUHGWKHPRVWELRDFWLYHFRQVWLWXHQWVDQGNQRZQIRUWKHLUDQWLLQÀDPPDWRU\DQWLVHSWLF
DQWLSKORJLVWLFDQGVSDVPRO\WLFSURSHUWLHV7KHHVVHQWLDORLO\LHOGDQGFRPSRVLWLRQLVDIIHFWHG
E\WKHJHQRW\SHWKHHQYLURQPHQWDOFRQGLWLRQVWKHFXOWLYDWLRQSUDFWLFHVHWF7KHLQFUHDVLQJ
demand for chamomile-based products, resulted in the development of high yielding varieties,
concerning the essential oil yield and the content of bioactive compounds, and the increase of
FKDPRPLOHFXOWLYDWLRQDOORYHUWKHZRUOG&KDPRPLOHFURSVDGRSWLQJORZLQSXWIDUPLQJDQG
RUJDQLFDJULFXOWXUDOSUDFWLFHVLVDFKDOOHQJHIRUWKHSURGXFWLRQRIKLJKTXDOLW\UDZPDWHULDO
DQGLQDFFRUGDQFHWRWKHLQGXVWU\GHPDQGV,QWKHSUHVHQWVWXG\WKHHIIHFWRILUULJDWLRQDQG
RUJDQLFIHUWLOL]DWLRQRQWKHHVVHQWLDORLOFRPSRVLWLRQRIDORFDO&KDPRPLOHFXOWLYDUFXOWLYDWHG
LQH[SHULPHQWDO¿HOGLQ7KHVVDO\&*UHHFHZDVLQYHVWLJDWHG7KHUHVXOWVVKRZHGWKDWĮ
ELVDEROROFRQWHQWZDVKLJKHUXQGHULUULJDWLRQFRQGLWLRQVWKRXJKWKHHIIHFWRIIHUWLOL]DWLRQZDV
QRWVLJQL¿FDQWFRPSDUHGWRWKHFRQWUROQRWUHDWPHQW2QWKHRWKHUKDQGWKHFKDPD]XOHQH
yield in the essential oil was increased under rainfed conditions and application of the lower
OHYHOVRIRUJDQLFIHUWLOL]DWLRQ
Key words:&KDPRPLOHHVVHQWLDORLOIHUWLOL]DWLRQLUULJDWLRQ

PHENOTYPIC EVALUATION OF SIDERITIS SCARDICA

Gris. SELECTED CLONES, AND PRE-BREEDING
EVALUATION FOR YIELD POTENTIAL AND
QUALITATIVE TRAITS
Eirini Sarrou1, Nikos Ploumidis2, Kostas Antoniadis2, Paschalina
Chatzopoulou1, Eleni Abraham2

+HOOHQLF$JULFXOWXUDO2UJDQL]DWLRQ³'(0(7(5´,QVWLWXWHRI3ODQW%UHHGLQJDQG*HQHWLF
5HFRXUVHV'HSDUWPHQWRI0HGLFLQDODQG$URPDWLF3ODQWV7KHUPL7KHVVDORQLNL
Greece,
2
/DERUDWRU\RI5DQJH6FLHQFH'HSDUWPHQWRI)RUHVWU\1DWXUDO(QYLURQPHQW$ULVWRWOH
8QLYHUVLW\RI7KHVVDORQLNL7KHVVDORQLNL*UHHFH
Sideritis scardica *ULV /DPLDFHDH FRPPRQO\ NQRZQ DV 0RXQWDLQ WHD LV DQ HQGHPLF WR
%DONDQ FRXQWULHV PHGLFLQDO DQG DURPDWLF VSHFLHV ,W PDLQO\ WKULYHV LQ ORZ IHUWLOLW\ VRLOV LQ
PRXQWDLQRXVDUHDVDQGDWDOWLWXGHVDERYHPZKLOHLVFRQVLGHUHGWREHWKUHDWHQHG
GXHWRRYHUH[SORLWDWLRQUHVXOWLQJRQVKULQNLQJWKHSRSXODWLRQ¶VVL]HLQQDWXUDOKDELWDWV/DWHO\
the increased number of reports on Sideritis VS ELRDFWLYLWLHV LH DQWLR[LGDQW DQWLEDFWHULDO
DQWLLQÀDPPDWRU\SURWHFWLYHWRQHXURGHJHQHUDWLYHGLVHDVHVHWFDQGLWVWUDGLWLRQDOXVHVDJDLQVW
JDVWURLQWHVWLQDOGLVRUGHUVWULJJHUHGWKHLQGXVWULDOGHPDQGIRUKLJKTXDOLW\UDZPDWHULDOZLWK
VXSHULRU TXDOLWDWLYH FKDUDFWHULVWLFV 7KH SUHVHQW VWXG\ UHSRUWV WKH SUHEUHHGLQJ HYDOXDWLRQ
of a promising S. scardica population, selected among several others native to Greece, for
H[SUHVVLQJ VXSHULRU SKHQRW\SLF WUDLWV 7KUHH FORQHV ZHUH GHYHORSHG IURP WKUHH LQGLYLGXDO
plants, respectively, which were initially selected for their superior morpho-metric traits,
in order to assess their morphological and agronomic characteristics, yield potential and
TXDOLWDWLYH WUDLWV $OO WKH PHDVXUHPHQWV ZHUH FRQGXFWHG DW WKH VWDJH RI IXOO EORRPLQJ RQ
LQGLYLGXDOV FORQH $ VWDWLVWLFDO VLJQL¿FDQW YDULDWLRQ ZDV GHWHFWHG EHWZHHQ WKH FORQHV
FRQFHUQLQJ WKH WLPH RI EORRPLQJ WKH OHQJWK RI VWHPV DQG LQÀRUHVFHQFH WKH IUHVK DQG GU\
ZHLJKWRIELRPDVVSURGXFWLRQLQGLFDWLQJKLJKSKHQRW\SLFGLYHUVLW\RIWKHVWDUWLQJSRSXODWLRQ
7KHJHQHWLFDQGFKHPLFDOFKDUDFWHUL]DWLRQRIWKHLQGLYLGXDOSODQWVLQWKHFORQHVLQFRPELQDWLRQ
ZLWKSKHQRW\SLFZLOOGHWHUPLQHVXSHULRUJHQRW\SHVIRUIXWXUHEUHHGLQJSXUSRVHV
Key words: Sideritis scardica, pre-breeding, clones, phenotyping

PHYTOCHEMICAL INVESTIGATIONS OF THREE

DIFFERENT ACCESSIONS OF PERILLA FRUTESCENS
(L.) Britt.
Hiwa M. Ahmed1,2, Szilvia Tavaszi-Sárosi13pWHU5DGiFVL1eYD1pPHWK
Zámbori1

6]HQW,VWYiQ8QLYHUVLW\)DFXOW\RI+RUWLFXOWXUDO6FLHQFH'HSDUWPHQWRI0HGLFLQDODQG
$URPDWLF3ODQWV9LOOiQ\LVWU%XGDSHVW+XQJDU\
2
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.XUGLVWDQ5HJLRQDO*RYHUQPHQW,UDT
Perilla frutescens/%ULWWLVDQDQQXDODURPDWLFSODQWWKDWEHORQJVWR/DPLDFHDHIDPLO\DQG

has been used traditionally to cure a number of human ailments as well as culinary herb and
VSLFHV7KHDLPRISUHVHQWVWXG\ZDVWRFRPSDUHWKHSURGXFWLRQDQGVHFRQGDU\PHWDEROLWHV
of three morphologically different Perilla frutescensDFFHVVLRQV3IGDUNJUHHQOHDYHV3I
EULJKW JUHHQ OHDYHV DQG 3I SXUSOH OHDYHV 3ODQWV KDYH EHHQ JURZQ LQ FOLPDWLFFKDPEHU
XQGHUFRQWUROOHGFRQGLWLRQKGD\QLJKW&5+7KHHVVHQWLDORLOFRQWHQW
DQG FRPSRVLWLRQ WRWDO SRO\SKHQRO FRQWHQW 73& DV ZHOO DV DQWLR[LGDQW FDSDFLW\ $2& RI
WKHOHDYHVZHUHLQYHVWLJDWHG7KH3IVKRZHGWKHKLJKHVWHVVHQWLDORLOFRQWHQWPLg
ZKLOH RWKHUV VKRZHG ORZHU DFFXPXODWLRQ OHYHO PL J 3I PL J
3IUHVSHFWLYHO\7KHHVVHQWLDORLOFRPSRVLWLRQZDVLGHQWL¿HGE\JDVFKURPDWRJUDSK\±PDVV
VSHFWURPHWU\*&06:HIRXQGSHULOODOGHK\GHDQGOLPRQHQHDVDPDMRU
HVVHQWLDORLOFRPSRQHQWRI3IZKLOHLQ3ISHULOODNHWRQHDQGOLPRQHQH
DQGLQ3IGHK\GURHOVKROW]LDNHWRQHDQGVKLVRIXUDQZHUHGHWHFWHGLQWKH
KLJKHVWUDWLR7KHKLJKHVW73&PJJ*$(GPZDVPHDVXUHGLQ3IIROORZHGE\3I
PJJ*$(GPDQGWKHORZHVWYDOXHZDVGHWHFWHGLQ3IPJJ*$(,QWHUPV
RI$2&3IPJ$$(JGPVKRZHGKLJKHUYDOXHVFRPSDUHGWR3IPJ$$(
gGPDQG3IPJ$$(JGP%DVHGRQWKHUHVXOWVIRUHVVHQWLDORLOV3IDQGIRU
SRO\SKHQROVWKH3IFRXOGEHFRQVLGHUHGIRUIXUWKHUSKDUPDFRORJLFDOVWXGLHV
Key words:DQWLR[LGDQWHVVHQWLDORLO/DPLDFHDHSRO\SKHQRO

OPTIONS OF HERBICIDES PROTECTION OF MARIAN

THISTLE (SILYBUM MARIANUM L.)
$QWRQtQ9DFXOtN1+HOHQD3OXKiþNRYi2, Jana Benešová2

$JULWHF3ODQW5HVHDUFK/WG'HSDUWPHQW3ODQW3URWHFWLRQV=HPČGČOVNi&=
âXPSHUN&]HFK5HSXEOLF0HQGHO8QLYHUVLW\LQ%UQR
2
Department of Crop Science, Breeding and Plant Medicine, Faculty ofAgriSciences,
=HPHGHOVND%UQR&]HFK5HSXEOLF
Silybum marianum / LV D VXI¿FLHQWO\ UREXVW DQG FRPSHWLWLYH SODQW DJDLQVW PRVW RI ZHHGV
%XWHVSHFLDOO\LQWKHHDUO\JURZWKDQGGHYHORSPHQWVWDJHVRIJURZWKLWFDQEHVLJQL¿FDQWO\
VXSSUHVVHGE\TXLFNO\HPHUJLQJDQQXDOPRQRFRWVDQGEURDGOHDYHGZHHGV$WWKHVDPHWLPH
SHUHQQLDO ZHHGV FDQ EH D ELJ SUREOHP ,Q RXU FRQGLWLRQV FRXFK JUDVV DQG WKLVWOH VRZQ DUH
SUREOHP $OWKRXJK LQ WKH SDVW WKHUH ZDV DOVR SHUIRUPHG PHFKDQLFDO FXOWLYDWLRQ KRHLQJ
FXUUHQWO\LQWKHFDVHRIRFFXUUHQFHRIZHHGVFKHPLFDOSURWHFWLRQLVFKRVHQLHXVLQJDVXLWDEOH
DQGUHJLVWHUHGKHUELFLGH&XUUHQWO\IRUDSSOLFDWLRQRQSylybum marianum permitted herbicides
DUH67203(ZLWKDLSHQGLPHWKDOLQDSSOLHGSUHHPHUJHQWO\WKHQ5(),1(6;ZLWK
DLWKLIHQVXOIXURQPHWK\ODQG%HWDQDOPD[[3URUHVSHFWLYHO\%HWDQDO0D[[3UR2'ZLWK
DLGHVPHGLSKDPHWKRIXPHVDWHSKHQPHGLSKDPDQGOHQDFLO7KHVHIRUPXODWLRQVDUHGHVLJQHG
IRU SRVWHPHUJHQFH UHVSHFWLYHO\ HDUO\ SRVWHPHUJHQFH WUHDWPHQW ,W LV LQWHUHVWLQJ WKDW WKHUH
LV QR UHJLVWHUHG JUDPLQLFLGH IRU FRQWUROOLQJ RI DQQXDO DQG SHUHQQLDO PRQRFRWV ZHHGV 2XU
H[SHULPHQWVVKRZHGWKDWQRUPDOUDQJHRIJUDPLQLFLGHVXVHGLQWKH&]HFK5HSXEOLFLVDKLJKO\
selective to Silybum marianumSODQWVHYHQDWGRVHUDWHFRQWUROOLQJFRXFKJUDVV
Key words: Sylibum marianum/KHUELFLGHVVHOHFWLYLW\\LHOG

AUXIN APPLICATION TO ENHANCE ROOTING OF

DALMATIAN SAGE, IMMORTELLE AND LAVANDIN
Smiljana Goreta Ban1,2*YR]GHQ'XPLþLü3

,QVWLWXWHRI$JULFXOWXUHDQG7RXULVP&DUOR+XJXHV3RUHþ&URDWLD
2
&HQWUHRI([FHOOHQFHIRU%LRGLYHUVLW\DQG0ROHFXODU3ODQW%UHHGLQJ6YHWRãLPXQVND
=DJUHE&URDWLD
3
7KH&URDWLDQFRDVWDQGLVODQGVDUHNQRZQE\LWVULFKQHVVRIQDWXUDOO\RFFXUULQJDURPDWLFDQG
PHGLFLQDO SODQWV 7KH 'DOPDWLDQ VDJH 6DOYLD RI¿FLQDOLV / DQG LPPRUWHOOH Helichrysum
italicum5RWK*'RQDUHQDWLYHSODQWVGLVWULEXWHGDORQJFRDVWDQGLVODQGVZKLOHODYDQGLQ
(Lavandula x intermedia (PHULF H[ /RLVHO LV VXFFHVVIXOO\ JURZQ FRPPHUFLDOO\ DQG LQ
JDUGHQV7KHDLPRIWKLVZRUNZDVWRWHVWWKHHI¿FDF\RIDX[LQVIRUURRWLQJRISODQWVFROOHFWHG
IURP QDWXUDO KDELWDW LQ &URDWLD 7KH VDJH DQG LPPRUWHOOH ZHUH FROOHFWHG DW RQH ORFDWLRQ
ZKHUHDV ODYDQGLQ ZDV FROOHFWHG DW WKUHH ORFDWLRQV DW LVODQG +YDU7KH FXWWLQJV ZHUH GLSSHG
LQVROXWLRQFRQWDLQLQJLQGROHDFHWLFDFLG,$$DWPJ/, indole-3-butyric acid (IBA)
PJ/QDSKWKDOHQHDFHWLFDFLG1$$DWPJ/, or tap water (control), and grown
XQGHUFRQGLWLRQVIDYRXUDEOHIRUURRWLQJ7KHURRWLQJSHUFHQWDJHRIVDJHFXWWLQJVZDVHQKDQFHG
E\ DSSOLFDWLRQ RI DX[LQV WR FRPSDUHG WR FRQWURO DQG VLPLODU WUHQG ZDV
REVHUYHGIRUQXPEHURIURRWVSHUFXWWLQJ$SSOLFDWLRQRI,$$RU,%$LQFUHDVHGVDJHURRWOHQJWK
FRPSDUHG WR FRQWURO RU 1$$ 7KH URRWLQJ SHUFHQWDJH RI LPPRUWHOOH ZDV XQGHU ZKHQ
,$$DQGFRQWUROZHUHDSSOLHGZKLOH1$$DSSOLFDWLRQUHVXOWHGLQURRWHGFXWWLQJV7KH
application of NAA increased number of roots, but no effect of auxins on immortelle cutting
URRWOHQJWKZDVREVHUYHG7KHUHZDVQRGLIIHUHQFHLQURRWLQJSHUFHQWDJHRIODYDQGLQFXWWLQJV
FRQVLGHULQJDX[LQDSSOLFDWLRQFRPSDUHGWRFRQWUROKRZHYHUVLJQL¿FDQWO\KLJKHUSHUFHQWDJHRI
rooted cuttings was observed for landraces from Brusje and Velo grabje compared to landraces
IURP+YDU7KHDX[LQDSSOLFDWLRQLQFUHDVHGWKHQXPEHUDQGOHQJWKRIWKHURRWVSHUFXWWLQJRI
ODQGUDFHVIURP%UXVMHDQG9HORJUDEOMHFRPSDUHGWRRQHVIURP+YDU$X[LQDSSOLFDWLRQFDQ
VLJQL¿FDQWO\LQFUHDVHURRWLQJSHUFHQWDJHRIVDJHDQGLPPRUWHOOHDQGHQVXUHIDVWDQGWUXHWR
W\SHSURSDJDWLRQRIJHQRW\SHVZLWKGHVLUDEOHPRUSKRORJLFDODQGELRFKHPLFDOWUDLWV
Key words: propagation, transplants, hormone

BREEDING SYNTHETICS IN CARAWAY

(CARUM CARVI L.)
Daniel Becker, Frank Marthe
-XOLXV.KQ,QVWLWXWH,QVWLWXWHIRU%UHHGLQJ5HVHDUFKRQ+RUWLFXOWXUDO&URSV4XHGOLQEXUJ
Caraway (Carum carvi/VHHGVDUHSULPDULO\XVHGDVVSLFHRUWHD'XHWRHVVHQWLDORLOFRQWHQW

LWHDVHVJDVWURLQWHVWLQDODIÀLFWLRQV,QWKHSDVWFDUDZD\ZDVJURZQHQWLUHO\DVELHQQLDOSODQWV
XQWLODQQXDOFXOWLYDUVZHUHGHYHORSHGLQWKHWK+RZHYHUDQQXDOFXOWLYDUVIDLOWRUHDFK
WKH OHYHO RI \LHOG RI ELHQQLDO FDUDZD\ GRZQ WR WKH SUHVHQW GDWH 7KHUHIRUH ZH HQGHDYRXU
WRWU\QRYHOZD\VRIEUHHGLQJIRUFDUDZD\&DUDZD\EUHHGLQJXVXDOO\FRQGXFWHGSRSXODWLRQ
EUHHGLQJ WR LPSURYH FXOWLYDUV ,Q DGGLWLRQ OLQH EUHHGLQJ ZDV LPSOHPHQWHG WR JDLQ PRUH
KRPRJHQHRXVSRSXODWLRQV%HVLGHV'+WHFKQRORJ\KDVEHHQGHYHORSHG7KLVPLJKWIDFLOLWDWH
K\EULGEUHHGLQJLQIXWXUHWRH[SORLWKHWHURVLV7KRXJKODFNLQJDV\VWHPIRUSROOLQDWLRQFRQWURO
EUHHGLQJRIV\QWKHWLFFXOWLYDUVLVWHVWHGDVD¿UVWDSSURDFK)RUWKLVSXUSRVHDSRO\FRVVZLOOEH
SHUIRUPHGRILQEUHGOLQHVDQGDQQXDOFXOWLYDUVDVVWDQGDUGV3HUIRUPDQFHRILQEUHGOLQHV
FXOWLYDUVDQGSURJHQLHVZLOOEHWHVWHGLQVXEVHTXHQW\HDUVWRPHDVXUHWKHJHQHUDOFRPELQLQJ
ability (GCA) and to get a notion of the actual heterosis effect, which can be exploited by
EUHHGLQJV\QWKHWLFV
Key words: caraway, Carum carvi, breeding synthetics

CULTIVATION PRACTICES OF THE FIRST LARGE

SCALE INDOOR FACILITIES FOR PRODUCTION OF
HIGH THC CANNABIS IN REPUBLIC OF MACEDONIA
G. Stefkov1, Z. Keskovski20'DUNRYVND6HUD¿PRYVND3

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)DFXOW\RI0HGLFDOVFLHQFHV8QLYHUVLW\³*RFH'HOFHY´6KWLS5HSXEOLFRI0DFHGRQLD
Cannabis (Cannabis sativa/&DQQDEDFHDHLVDZHOONQRZQGUXJDQGDFRQWUROOHGVXEVWDQFH

ZKLFK SRVVHVVLRQ DQG XVH DUH LOOHJDO LQ PRVW FRXQWULHV RI WKH ZRUOG 1RZDGD\V WKH XVH RI
FDQQDELVDQGLWVOHJDOL]DWLRQIRUPHGLFDOXVHKDVEHFRPHDZRUOGZLGHWUHQG/DZVDQGDWWLWXGHV
WRZDUGFDQQDELVDUHFKDQJLQJWKHVHGD\V/HJDOL]DWLRQRIFDQQDELVXVHIRUPHGLFDOSXUSRVHV
is a hot topic at the global level and in most countries, there have been initiatives to amend the
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UHODWHGSURGXFWVV\QWKHWLFDOO\SURGXFHGDYDLODEOHWRSDWLHQWV,QFKDQJHVRIWKH/DZ
IRUQDUFRWLFVXEVWDQFHVLQ50ZHUHPDGHFRQFHUQLQJOHJDOL]DWLRQRIFDQQDELVIRUPHGLFLQDO
DQGVFLHQWL¿FSXUSRVHVLQDZD\RIFXOWLYDWLRQSURGXFWLRQRIFDQQDELVPHGLFDOSUHSDUDWLRQV
DV ZHOO DV LPSRUW RI SKDUPDFHXWLFDOV DQG FRVPHWLFV FRQWDLQLQJ FDQQDELV RU FDQQDELQRLGV
7KHUHIRUHGXHWRWKHLQFUHDVHGJOREDOQHFHVVLW\RIPHGLFDOFDQQDELVZLWKKLJK7+&FRQWHQWLQ
RUGHUWRUHVSRQGWRWKHFKDOOHQJHVDULVLQJIURPWKLVLVVXH1<6.+ROGLQJKDYHVWDUWHGDODUJH
VFDOHLQGRRUSURGXFWLRQRIGLIIHUHQWYDULHWLHVRIFDQQDELVXVLQJVHYHUDOFXOWLYDWLRQSUDFWLFHV
Alongside the production of plant material, from indigenous and foreign origin, cannabis
H[WUDFWVPDLQO\FDQQDELVRLODQGYDULRXVSURGXFWVDUHSUHSDUHG
Key words: medicinal cannabis, legal growing

SPONSORS AND DONATORS

Zračna luka Split d.o.o.

BOTI COMMERCE D.O.O. CEKOM 3LJ
OPĆINA MUĆ OPĆINA KISTANJE

Book of Abstracts, 2018

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10th CMAPSEEC:

May 20-24, 2018, Split, Croatia

Jointly organized by:

Association for Medicinal and Aromatic Plants of Southeast European

Dear Friends and Colleagues,

President of the Organizing Commitee

Poster Section M-1 (Monday, May 21st,14:30-15:30)

P-11(OLQD<DQNRYD7VYHWNRYD,YDOHQD,OLHYDStoyan Stoyanov, Boriana Sidjimova,

P-13 Seçil Karahüseyin$\QXU6DUÕ

P-14 Ahmet Dogan

P-16 Ibraliu Alban*L[KDUL%HOXO.DGLDVL1DMDGD$JDVWUD$UOLQGD7DNR5HD+\VNR

P-19 Ina Aneva, (NDWHULQD.R]XKDURYD3HWHU=KHOHY6LPHRQ/XNDQRY0DULD3HQHYD

P-21 Peter Zhelev,QD$QHYD$QWRDQHWD7UHQGD¿ORYD0LOND7RGRURYD

Poster Section Ph-1 (Tuesday, May 22nd, 14:15-15:15)

Poster Section Ph-2 (Thursday, May 24th, 13:40-14:40)

Poster Section C-1 (Thursday, May 24th, 13:40-14:40)

I. MEDICINAL AND AROMATIC PLANTS ECOLOGY,

24 May 20-24, 2018, Split, Croatia

ETHNOBOTANY OF THE ADRIATIC ISLANDS IN

May 20-24, 2018, Split, Croatia 25

CHLOROPLAST DNA DIVERSITY AND

Key words: 6DOYLD RI¿FLQDOLV, Salvia lavandulifolia, phylogeography, chloroplast DNA,

26 May 20-24, 2018, Split, Croatia

WILD RASPBERRY FROM THE CENTRAL BALKAN:

Raspberry (Rubus idaeus/5RVDFHDH LVDZHOONQRZQVSHFLHVQDWLYHWR(XURSHDQGQRUWK

May 20-24, 2018, Split, Croatia 27

WILD LABIATES AROMATIC PLANTS AND THEIR BEE

28 May 20-24, 2018, Split, Croatia

THE USE OF MEDICINAL PLANTS IN THE KNIN AREA,

Key words: ethnobotany, PHGLFLQDOSODQWVWUDGLWLRQDONQRZOHGJH

May 20-24, 2018, Split, Croatia 29

ENVIRONMENTAL POLLUTION AND HEAVY METALS

Key words: heavy metals, analysis, accumulation, environmental pollution

30 May 20-24, 2018, Split, Croatia

STRONG INFLUENCE OF CLONALITY ON THE

May 20-24, 2018, Split, Croatia 31

MICROSATELLITE DIVERSITY OF DALMATIAN SAGE

32 May 20-24, 2018, Split, Croatia

MEDICINAL PLANTS AS A FOOD SOURCE FOR

May 20-24, 2018, Split, Croatia 33

GENETIC DIVERSITY OF IMMORTELLE

Immortelle (Helichrysum italicum5RWK*'RQ LVDSHUHQQLDOWKHUPRSKLORXVVXEVKUXEZLGHO\

Key words: Helichrysum italicumPHGLFLQDODQGDURPDWLFSODQWVJHQHWLFGLYHUVLW\$)/3V

34 May 20-24, 2018, Split, Croatia

GENETIC DIVERSITY OF NATURAL POPULATIONS OF

S. fruticose 0LOONQRZQLQWKHLQWHUQDWLRQDOWUDGHDV*UHHNVDJHDQGS. pomifera /. NQRZQDV

Key words: Salvia*UHHNVDJH&UHWDQVDJHPLFURVDWHOOLWHVSRSXODWLRQJHQHWLFV

May 20-24, 2018, Split, Croatia 35

CHEMOSYSTEMATICS OF THE CICHORIEAE TRIBE OF

36 May 20-24, 2018, Split, Croatia

AN ANALYSIS ON WILD EDIBLE PLANTS OF THE

University of Marmara, Faculty of Pharmacy, Department of Pharmaceutical Botany,

May 20-24, 2018, Split, Croatia 37

GENETIC BACKGROUND OF DALMATIAN

38 May 20-24, 2018, Split, Croatia

PREDICTING SUITABLE HABITAT FOR DALMATIAN

Dalmatian pyrethrum (Tanacetum cinerariifolium7UHYLU6FK%LS LVDWKHUPRSKLOLFSODQW

Key words: suitable habitat modelling, Tanacetum cinerariifoliumPD[HQWPRGHO%DONDQ

May 20-24, 2018, Split, Croatia 39

ASSESSING THE MEDICINAL AND AROMATIC PLANTS

Key words: watershed, Devolli River, MAPs

40 May 20-24, 2018, Split, Croatia

THE MEDICINAL AND WILD FOOD PLANTS OF

May 20-24, 2018, Split, Croatia 41

P-11(OLQD<DQNRYD7VYHWNRYD,YDOHQD,OLHYDStoyan Stoyanov, Boriana Sidjimova,

P-13 Seçil Karahüseyin$\QXU6DUÕ

P-16 Ibraliu Alban*L[KDUL%HOXO.DGLDVL1DMDGD$JDVWUD$UOLQGD7DNR5HD+\VNR

P-19 Ina Aneva, (NDWHULQD.R]XKDURYD3HWHU=KHOHY6LPHRQ/XNDQRY0DULD3HQHYD

P-21 Peter Zhelev,QD$QHYD$QWRDQHWD7UHQGD¿ORYD0LOND7RGRURYD

Raspberry (Rubus idaeus/5RVDFHDHLVDZHOONQRZQVSHFLHVQDWLYHWR(XURSHDQGQRUWK

Key words: ethnobotany, PHGLFLQDOSODQWVWUDGLWLRQDONQRZOHGJH

Immortelle (Helichrysum italicum5RWK*'RQLVDSHUHQQLDOWKHUPRSKLORXVVXEVKUXEZLGHO\

Key words: Helichrysum italicumPHGLFLQDODQGDURPDWLFSODQWVJHQHWLFGLYHUVLW\$)/3V

S. fruticose 0LOONQRZQLQWKHLQWHUQDWLRQDOWUDGHDV*UHHNVDJHDQGS. pomifera /. NQRZQDV

Key words: Salvia*UHHNVDJH&UHWDQVDJHPLFURVDWHOOLWHVSRSXODWLRQJHQHWLFV

Dalmatian pyrethrum (Tanacetum cinerariifolium7UHYLU6FK%LSLVDWKHUPRSKLOLFSODQW

Key words: suitable habitat modelling, Tanacetum cinerariifoliumPD[HQWPRGHO%DONDQ

Helleborus odorus:DOGVW .LWVXEVScyclophyllus $%UDXQ0DLUH 3HWLWPLVNQRZQ

Bryophytes DUH WKHVHFRQG ELJJHVWJURXS RI ODQG SODQWVDIWHUDQJLRVSHUPV+RZHYHUWKH\

Juniperus communis /DZLOGVKUXEVSHFLHVJURZLQJHVSHFLDOO\LQPRXQWDLQDUHDVRI$OEDQLD

Key words: Rhodiola rosea PRUSKRORJLFDO FKDUDFWHUL]DWLRQ DJURQRPLF FKDUDFWHUL]DWLRQ