Direct HDL-Cholesterol Kit (D-HDL-C)

Cat. 0200
Clearance method
Size: R1 (60ml×1) + R2 (20ml×1)
Intended Use: Blank is calibrated to zero.
For the quantitative determination of high-density lipoprotein Read Acalibrator and Asample.
cholesterol (HDL-C) in human Serum

Principle of reaction: Calculation：

1. Elimination of chylomicron (CM), LDL-cholesterol and Acalibrator

VLDL-cholesterol by cholesterol esterase, cholesterol
oxidase and catalase in Reagent 1. This is colorless reaction. Con. of HDL-C＝ -------- ×Con. of Calibrator
2. Specific reaction of HDL-cholesterol after release of HDL-C Asample (mmol/L or mg/dL)
by detergent in Reagent 2.
Components: Expected Values:
Reagent Content Concentration Adult Males: 1.16-1.42mmol/L (45-55mg/dL)
Cholesterol esterase
≥1000U/L Adult Females: 1.29-1.55mmol/L (50-60mg/dL)
Cholesterol oxidase
R1 ≥800U/L Regulation:＞1.04mmol/L (＞40mg/dL)
Catalase
≥900U/L
Low value:＜0.91mmol/L
Toos

4AA Conversions:

detergent mmol/L×38.67=mg/dL; mg/dL×0.02586=mmol/L

R2
Peroxidase (POD) The reference range should be determined by each hospital to
≥4000U/L
confirm with the characteristics of the region being tested.
NaN3
Linearity：
D-HDL-C calibrator serum(note on the label)
The test is linear up to 2.59mmol/L(100mg/dL)
Shelf Life:
12 months at 2～8℃, protecting from light.
Precautions：
Sample:
1. If working solution show purple color, discard the reagents.
Fresh Serum ， or EDTA and heparinized plasma on an empty 2. In vitro use only. Sodium azide is include, avoid ingestion or
stomach are the recommended specimens. Centrifuge and contact with skin or mucous membranes.
remove the serum or plasma as soon as possible after collection
(within 3 hours). 3. Human material used in formulating this calibrator has been tested
and found to be negative for HBs antigens, anti-HCV antibody, and
The sample is stable in 12 hours at room temperature(15~30℃), anti-HIV antibody. However, handle with care as patient sample
5 days at 2~8℃, or 1 month long when kept frozen in -20 ℃ or preparation is recommended.
below. Once frozen or melted, it shall not be refrozen. Extreme
turbidity serum should be diluted before assaying. 4. Shall be stored at 2 ～ 8℃. Avoid being stored at temperature
over 8℃ or below 0℃.
Assay conditions:
Wavelength：546nm Bibliography：
Temperature：37℃
Cuvette：1cm. 1. 《Expected Values Handbook of Laboratory Medicine》, Li-hua Zhu
Procedure:
1998, (in Chinese)
Ready to use.

Blank Calibrator Sample

Registration number：SFDA-3050260(2003)
Sample -- -- 6μl Standard number：YZB/GB 0667-2003
Calibrator -- 6μl --

Water 6μl -- -- Biosino Bio-Technology and Science Inc.

R1 600μl 600μl 600μl Add：No.27, Chaoqian Road, Changping District,
102200 Beijing, China
Incubate 5 minutes at 37℃
Tel：+86-10-69722840, Fax：+86-10-69722840
R2 200μl 200μl 200μl Website：www.zhongsheng.com.cn
Email: trade@zhongsheng.com.cn
Incubate 5 minutes at 37℃