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Cristina S.C. Calheiros , António O.S.S. Rangel & Paula M.L. Castro
To cite this article: Cristina S.C. Calheiros , António O.S.S. Rangel & Paula M.L. Castro (2014)
Constructed Wetlands for Tannery Wastewater Treatment in Portugal: Ten Years of Experience,
International Journal of Phytoremediation, 16:9, 859-870, DOI: 10.1080/15226514.2013.798622
Wastewaters from tannery industry are complex in composition and providing adequate treat-
ment can be difficult. Constructed wetlands (CW) are regarded as an alternative treatment
to the conventional biological systems, as a developing cost-effective and environmentally
friendly phytoremediation technology. The present review compiles and integrates informa-
tion on CWs technology for the needs of the tannery sector. The following issues arise as
crucial for the implementation of such systems, namely i) an accurate wastewater character-
ization and an effective pretreatment before reaching the CW, ii) choosing the plants species
better adapted to the imposed conditions, iii) substrate selection and iv) range of organic
loadings applied. The examples practiced in Portugal give indication that horizontal subsur-
face flow systems, with expanded clay media, are a suitable option to be considered when
dealing with high organic loading tannery wastewater (up to c.a. 3800 kgCODha−1d−1), be-
ing resilient to a wide range of hydraulic variations. Plants such as Phragmites and Typha
have shown to be adequate for tannery wastewater depuration, with Arundo donax proving
resilient to high salinity wastewaters. The flexibility of implementation allows the CW to be
adapted to different sites with different configurations, being suitable as main secondary or
tertiary treatment stage.
KEY WORDS: Constructed wetland, tannery wastewater, phytoremediation, leather industry, wastewater treat-
ment, Portugal
INTRODUCTION
The leather or tannery industry represents for many countries an important economic
player. Based on 2008 figures, apart from EU, the major leather production centers in the
world are found in Mexico, Argentina, Brazil, South Korea, China, India, and Pakistan
(EC 2012). In the 20th century most of the tanneries changed the production through the
replacement of the traditional vegetable tanning into the chromium tanning technology.
The Portuguese leather industry has 60 active companies; typically small and medium
859
860 C. S. C. CALHEIROS ET AL.
Table 1 Typical physico-chemical characteristics of tannery wastewater from Portuguese sector. Range values
(min.-max.) are presented
Secondary 7.2–8.20 13.3–19.3 68–285 16–88 40–146 3.9–34.2 0.55–3.00 Calheiros et al.
(2012a)
Primary 4.76–8–26 4.74–9.64 835–2261 430–850 43–110 102–160 0.13–0.83 Calheiros et al.
(2009b)
Primary 4.43–8.46 4.74–10.05 808–2449 420–1000 32–324 87–160 0.1–0.95 Calheiros et al.
(2008a)
Primary 4.64–8.75 n.d. 1108–3141 727–1300 33–125 90–230 0.08–0.45 Calheiros et al.
(2007)
that had primary treatment, and between 0.10 and 0.65 for waters after secondary treat-
ment. The biodegradability of the tannery wastewaters in CWs is affected by their intrinsic
specificity (Calheiros et al. 2007). Besides that, biodegradation is limited by the recalcitrant
properties of the wastewater (Calheiros et al. 2009a). The wastewater characterization is
thus imperious in order to determine the treatment level needed.
Most CWs considered in this review were designed for secondary treatment of tannery
wastewater being the main issue the high organic content (Calheiros et al. 2007, 2008a,
2009c). Two systems have also been setup with the purpose of tertiary treatment of high
salinity tannery wastewater, after a conventional primary treatment followed by a secondary
treatment by means of activated sludge (Calheiros et al. 2012a).
Vegetation
Macrophyte species selection plays an important role and may compromise the treat-
ment if unsuitable for such purpose (Brisson and Chazarenc 2009). According to available
data (Dias et al. 2006) the most frequently used plants in Portuguese CW are P. australis,
T. latifolia, Iris pseudacorus, Juncus effusus, and Cyperus spp. However these data refers
mostly to municipal and domestic type wastewater. In a scenario of industrial wastewater,
such as tannery, Calheiros et al. (2007) reported that I. pseudacorus, Canna indica, and
Stenotphrum secundatum were not suitable for this purpose since their survival rates were
not satisfactory to comply with an effective treatment. On the other hand, P. australis and T.
latifolia have shown to proliferate when fed with the same tannery wastewater (Calheiros
et al. 2007, 2008a, 2009c). Thus, even assuming that implementation and dimensioning of
the CW system has been carefully undertaken, plant adaptation and development may be
compromised due to toxic levels of contaminants and nutrient deficiency occurring in this
type of wastewater. Even when plants survive, data on the relation between plant species
and contaminants removal available in the literature is diverse since there are studies that
did not show significant differences between the used plants and removal efficiency (e.g.,
Calheiros et al. 2009c) while others have reported such differences (e.g., Brisson and Chaz-
arenc 2009). For instance Calheiros et al. (2012a) reported good performance of CW planted
with Sarcocornia spp. and Arundo donax concerning nutrient removal and plant adaptation
in polishing tannery wastewater coming from a conventional wastewater treatment plant,
however A. donax was considered the most promising to be used due to its deeper root zone,
vigorous growth and higher capacity to uptake nutrients (P and N). In sum, plants with high
Table 2 Operational characteristics of constructed wetland (CW) systems for tannery wastewater in Portugal
R
Tertiary 2 CW with 3 cells HSF 72 6, 21 Arundo donax Leca NR + sand Calheiros et al. (2010, 2012a)
Sarcocornia fruticosa
R
Secondary 2 CW with 2 cells HSF 2.4 6, 8, 18 Typha latifolia Leca MR Calheiros et al. (2009a, 2009c)
Phragmites australis
R
Secondary 3 CW HSF 1.2 6, 8, 18 Typha latifolia Leca MR, NR, gravel Calheiros et al. (2008a, 2008b)
R
Secondary 5 CW HSF 1.2 3, 6 Canna indica Leca MR Calheiros et al. (2007)
Iris pseudacorus
Phragmites australis
Typha latifolia
Stenotaphrum secundatum
863
864 C. S. C. CALHEIROS ET AL.
Figure 1 Constructed wetland planted with Arundo donax for tannery wastewater polishing
biomass, such P. australis, T. latifolia, and A. donax, have shown to have good capacity to
uptake nutrients (Calheiros et al. 2009c, 2012a) and other metal pollutants found in tannery
wastewater (Bragato et al. 2004; Calheiros et al. 2008b) bringing more efficiency to the
treatment system. Even within the same industrial sector, the plants selected to be used in
CW may be different. For instance Calheiros et al. (2012a), when dealing with high salinity
tannery wastewater, selected Arundo donax (Figure 1) and Sarcocornia, plants that due to
their intrinsic characteristics would be expected to be resilient to the imposed conditions.
Bassica indica is an example of other species that tolerate high salinity as presented by
Shelef et al. (2012).
Wetland Media
The media in CW with subsurface flow should be effective in providing a good
support for plants development, establishment and propagation, for microbial communities
attachment and a satisfactory hydraulic conductivity. If possible, it should be acquired
locally in order to reduce the transportation costs and potentiate local economy. Studies
undertaken in Bangladesh (Saeed et al. 2012), when employed locally available substrates
(i.e., organic coco-peat, cupola slag, and pea gravel) showed different results that have been
in part related to the matrices porosity. Calheiros et al. (2009b) concluded that the type
of substrate and the presence of T. latifolia had a major effect on the species richness and
the structure of bacterial communities, when testing three substrates (two expanded clay
aggregates and one type of gravel) (Figure 2) and one unplanted unit. The type of substrate
CONSTRUCTED WETLANDS FOR TANNERY WASTEWATER IN PORTUGAL 865
Figure 2 Constructed wetland pilot units planted with Typha latifolia in a) Filtralite
R
MR 3–8, b) fine gravel -
R
AGH 4–8 and c) Filtralite NR 3–8.
may also influence the plant propagation and the organic removal from the wastewater
(Calheiros et al. 2008a).
Table 3 Constructed wetlands treatment efficiencies concerning organic loadings (OL) and organic removals
(OR)
OL OR OL OR
(kg BOD (kg BOD (kg COD (kg COD
CW plant and substrate ha−1 d−1) ha−1 d−1) ha−1 d−1) ha−1 d−1) Reference
have claimed that CW may be considered a viable and optional technology for chromium
removal in tannery effluent. Records of Cr removal efficiencies of 90 to 99% (5 mg/L at
the inlet) (Dotro et al. 2010), of 99.83 ± 0.19 (371.70 ± 44.48 mg/L at the inlet) (Kaseva
and Mbuligwe 2010) and of 97 to 100% (2.2–3 mg/L at the inlet) (Tiglyene et al. 2005)
have been published. Caution must be taken with the concentration in vegetal detritus after
prune maintenance is undertaken.
When addressing the phosphorus and nitrogen removal, it has been reported that
the performance is lower when compared to the organics removal, although in some way
expected for this type of systems (Kadlec et al. 2000). For example, Calheiros et al. (2008a),
reported that for an inlet of 0.21–0.43 mg P/L the outlet was 0.18–0.35 mg P/L and for an
inlet of 63–87 mg NH3 -N/L the outlet was 40–56 mg NH3 -N/L. Removal of PO4 reaching
61% (inlet 15 (±7) mg/L, hydraulic retention time = 12.5 d) has been reported for the
treatment of tannery wastewater by CW in Bangladesh (Saeed et al. 2012). High removal
of NH4 -N (99%) from tannery effluents has been reported by Kucuk et al. (2003) for an
inlet of 20 mg/L at a hydraulic retention time of 7 days. Kucuk et al. (2003) has stated
that although COD and PO4 -P are not significantly affected by HRT the ammonia-nitrogen
removal is, which is an important factor when dimensioning the CW system. It is known
that tannery wastewater with high salt contents is difficult to be treated by conventional
biological means (Durai and Rajasimman 2011). Calheiros et al. (2012a) managed to prove
CWs efficiency for organic removal (up to 615 kg COD ha−1 d−1) in the presence of
high salinity. Shelef et al. (2012), when using Bassia indica in a CW, besides the plant
resilience, also registered salinity reduction by 20–60% in comparison with unplanted
systems or systems planted with other wetland plants.
As Faulwetter et al. (2009) emphasized, the most important factor that has influence
on the pollutant removal in CWs is the microbiology inherent to the system, however
there are not many detailed studies on the microbial communities established in such sys-
tems. On his review the prevalence of certain microbial groups related to the removal of
certain pollutants in CWs, such as nitrifiers, denitrifiers, and sulfate reducing and oxidiz-
ing bacteria, is stated. Bacterial community dynamics in horizontal flow CW for tannery
wastewater treatment was assessed by Calheiros et al. (2009a, 2009b) and also when
Table 4 Examples of the use of constructed wetlands for tannery wastewater in operation worldwide (adapted from Calheiros et al. 2012b)
Operational data Performance data
Bangladesh VF-HF-VF P. australis 0.65–1.33–0.65 6 (cm/d)/ 11500 ± 6000 200 ± 60 (98) 4200 ± 2800 80 ± 40 (98) — — Saeed et al. (2012)
4.8–12.5–2.4
India HF Typha — 47.5 (m3/d) / – 1347 – (78) 649 – (93) — — UNIDO (2001)
HF Reed bed — — 1751 579 (–) 776 117 (–) — — Emmanuel and Anand
(2007)
Italy FWS Carex spp. 60 160 (L/h) / 3–3.5 — — — — 0.41 – (71) Bragato et al. (2004)
Marocco VF P. australis 120 (L) 35 (mL/min) / – 530–1216 408–750 (–) — — 534–1000 2.2–3 (99) Tiglyene et al. (2005)
Mexico — Typha spp., S. 450 20 (L/min) / aprox. 12340–17520 68–598 675–1320 36–86 (93–95) 22–31 0.07–0.08 (99) Aguilar et al. (2008)
americanus 2 (96–98)
Portugal HF T. latifolia 1.2 6 (cm/d) / 3.4 1755–2669 600–915 740–1300 345–630 <0.010–0.027 <0.001–0.080 (–) Calheiros et al. (2007)
(54–73) (41–58)
HF P. australis 2.4 18 (cm/d) / 2 1354–2138 500–713 720–1000 353–441 0.021–0.885 0.009–2.63 (–) Calheiros et al.
(57–67) (48–59) (2009c)
3
HF A. donax 72 4 (m /d) / 2 68–285 27–120 16–88 (–) 4–16 (–) — — Calheiros et al.
(2012a)
Tanzania HF P. mauritianus 0.45 0.045 ± 0.005 — — — — 371.70 ± 44.48 0.69 ± 0.65 (99.83 Kaseva and Mbuligwe
(m3/d) / 1.8 ± 0.2 ± 0.19) (2010)
Turkey HF P. australis 378 –/8 210 – (30) — — 0. 20 0.01–0.05 (43–55) Kucuk et al. (2003)
UK HF G. maxima — –/5 1160 150 (87) — — — — Daniels (1998)
USA HF Typha spp. 0.31 11.25 (L/d) / – 980 — 526 5–220 (95–99) 5 0.05–0.2 (90–99) Dotro et al. (2010)
867
868 C. S. C. CALHEIROS ET AL.
high salinity conditions were present (Calheiros et al. 2010). Strains recovered from CWs
treating tannery wastewater have been identified as belonging to genera Pseudomonas,
Bacillus, Halomonas, and Paracoccus (Calheiros et al. 2009a, 2009b). Genera related
with bacteria present in CWs treating high salinity tannery wastewater include Algoripha-
gus, Bacillus, Halomonas, Staphylococus, Rheinheimera, Sphingomonas, Flavobacterium,
Pseudomonas, and Listonella (Calheiros et al. 2010). Main outcomes stated that the type
of substrate and the presence or absent of plants had a major effect on the species richness
and the bacterial communities structure. When different plants were considered, the type
of plant had also a major effect on the established bacterial communities.
CONCLUSIONS
The preservation of tannery industry is very important, especially to Portugal, due to
its historical and economic values. The sustainability of water management at an industrial
scale is directly related with the fluxes of the production process. The experience obtained
with CWs for tannery wastewater treatment has revealed that these systems act as a reliable
choice as secondary or tertiary treatment. The subsurface flow system with expanded clay
as main media and vegetated with high biomass producers, P. australis or T. latifolia or A.
donax has proved to be effective providing depuration of this type of wastewater.
The low cost of implementation, low maintenance and the fact that specialize per-
sonnel to operate them is not needed, are the main assets of the technology.
ACKNOWLEDGMENTS
Cristina S. C. Calheiros thank to Fundação para a Ciência e a Tecnologia (FCT,
Portugal) the grant SFRH/BPD/63204/2009. This work was also supported by National
Funds from FCT through project PEst-OE/EQB/LA0016/2011.
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