JONATHAN A.

SAWAY 4-BSDM-B

Laboratory Exercise 2

Familiarization of Laboratory Facilities and Equipment in a Biotech Laboratory

1. Laboratories housed at the Biotechnology Unit of Southern Mindanao Agricultural Research

Center (USMARC).

Laboratory Uses and Importance

1. Plant tissue culture
laboratory
2. Isozyme laboratory
3. Animal science laboratory
4. Virus-indexing laboratory
5. Molecular and biotechnology
laboratory
-used to produce clones of a plant in a method known as
micro propagation.
-To clean particular plants of viral and other infections
and to quickly multiply these plants as 'cleaned stock'
for horticulture and agriculture.
- performs electrophoretic analyses of genetically
conditioned enzyme variation by separating multiple
forms of enzymes in a solid matrix
- detection of genetic variation within and among
populations, the identification
of plant clones, hybrids and their distribution in an area,
and studies of
evolutionary dynamics in natural plant populations as
well as plant mating
systems and genetic diversity in rare or invasive species.
- housing, caretaking, genetic and microbial quality
control, anesthesia and analgesia, feeding, experimental
techniques, etc
- contribute to the reliability and reproducibility of the
results of animal experiments and to the life quality of
laboratory animals that are used for research.
-Production of virus antibody by molecular cloning and
expression technology
-Identification and characterization of plant viruses
occurring in economically important crops in, preparation
and preservation of virus antibodies, and development of
indexing techniques.
- it is already used widely in manufacturing insulin for
diabetics, and is central to a range of other biomedical
products.
-provides you with the right mixture of sound scientific
knowledge and commercial expertise to succeed in the
emerging world of biotechnology
2. Laboratory facilities that might be added to the laboratory at Biotech unit University of
Southern Mindanao Agricultural Research Center. (USMARC)

Laboratory Facilities Uses

1. Bioreactor -used for precise phototrophic cultivation of algae and
cyanobacteria. They feature a unique combination of the
cultivator and monitoring device. Light power and spectral
composition as well as the temperature and aeration gas
composition can be set with a high accuracy. The
illumination unit can be removed easily prior to sterilization
and then clicked back into position following the procedure.
2. CO2 Incubator -plate & sample drying problems are greatly reduced with
high humidity levels using this device. An anticipative logic
system controlling both temperature and CO2 levels.
3. Environmental -with programmable controller provides precise
Chamber temperature and humidity control.
4. FTIR The application of programmable gain amplifier, high
Spectrometer accuracy A/D converter and embedded computer improves
the accuracy and reliability of the whole system. It is
user friendly, rich function software enables easy,
convenient and flexible operation. Spectrum
collect, spectrum conversion, spectrum collect, spectrum
conversion, spectrum processing, spectrum analyzing, and
spectrum output function etc. can be performed.
5. Particle Counter -traditional design with measuring unit, computer and
oscillograph. Advance pulse measurement technology, more
than 8000 channels. It is replaced with a pressure sensor,
provides more accurate measurement and control of degree
of vacuum, and higher automation.
6. Particle Size -it is used to measure fog drops: Its structure can be
Analyzer changed according to the requirements. A Fourrier Lens
with different focal length can be used to change the
measuring range.
7. Plant Growth -are ideal for agriculture or biotechnology testing growth of
Chamber plants in an artificial climatic condition. It controls
temperature, relative humidity and illumination. The wide
variety of temperatures and light intensity patterns that are
essential in plant research can now be accurately
reproduced and controlled with the updated range by Plant
Growth Chambers.
8. X-Ray -it can simultaneously analyze elements ranging from Na to
Spectrometer 92U. It is a very rapid method with determination time from
10 seconds to 1000 seconds.
9. Safety Cabinets -are designed for balance applications in the safety storage
industry. Handling of harmful and toxic compounds are
done safely thus reducing the risk of explosion. Safety
Cabinets are high performance unit that are easy to use,
clean and maintain. Particular attention is also paid to
reliability and smooth running.
 10. Water -it is a compact system that purifies water for general
Purification System laboratory use or as a feed source for ultrapure system.
Utilizing tap water for feed.
3. List of available equipment in Biotech unit University of Southern Mindanao Agricultural
Research Center (USMARC).

Laboratory Facilities Uses

1 Thermal cycler - is a laboratory apparatus most commonly used to amplify
segments of DNA via the polymerase chain reaction (PCR)
2 Microcentrifuge - is a piece of laboratory equipment, driven by a motor,
which spins liquid samples at high speed. There are various
types of centrifuges, depending on the size and the sample
capacity.
3 Distilling Apparatus -a set of apparatuses that uses distillation, a common
operation in many laboratories for the purpose of
separating and/or purifying components of a liquid mixture
the apparatus used consists of three major
parts: distillation flask (or ‘pot’) to heat the mixture and
volatilize the components, a condenser to cool the vapors
back to liquid state, and a collection vessel.
4 Fumehood - is a type of local ventilation device that is designed to
limit exposure to hazardous or toxic
fumes,vapors or dusts.
5 Pipetor/micropipettor -is a laboratory tool commonly used in chemistry, biology
and medicine to transport a measured volume of liquid,
often as a media dispenser
6 Vortex - is a simple device used commonly in laboratories to mix
small vials of liquid.
7 Water bath -is a device or a vessel for regulating the temperature of
anything subjected to heat, by surrounding the vessel
containing it with another vessel containing water which
can be kept at a desired temperature.
8 Horizontal gel -also called Agarose gel electrophoresis because it uses
electrophoresis Agarose gel. It maintains a uniform electric field across
apparatus the gel, provide cooling to prevent thermal artifacts, and
allow access to the gel for sample loading and monitoring
the run.
9 Gel documentation - is equipment widely used in molecular biology
system laboratories for the imaging and documentation of nucleic
acid and protein suspended within polyacrylamide or
agarose gels. These gels are typically stained with
ethidium bromide or other fluorophores such as SYBR
Green
10 Polyacrylamide gel - a gel electrophoresis device that uses acrylamide gel that
electrophoresis is use in a separation method frequently
used to analyze DNA fragments generated by restriction
enzymes, and it
is a convenient analytical method for determining the size
of DNA molecules
in the range of 500 to 30,000 base pairs. It can also be
used to
separate other charged biomolecules such as dyes, RNA
 and proteins.
11 Microwave oven -is a device that heats food by bombarding it with
electromagnetic radiation in the spectrum causing
polarized molecules in the food to rotate and build up
thermal energy in a process known as dielectric heating.
12 Stirrer/hot plate -is a portable self-contained tabletop small appliance that
features one, two or more gas burners or electric heating
elements. A hot plate can be used as a stand-alone
appliance, but is often used as a substitute for one of the
burners from an oven range or the cook top of a stove.
13 Electronic digital -is a weighing scale that is used for measuring substances.
balance/Analytical It can be found in place where large quantities of food are
balance prepared like hotel kitchens and institutional kitchens.
People also use them at home. They are easier to use and
they provide more accurate readings.
14 Biofreezer -is a kind of freezer that user a topical over-the-
counter gel for pain relief consisting of menthol with and
other ingredients.
15 Ice maker - may refer to either a consumer device for making ice,
found inside a home freezer; a stand-alone appliance for
making ice, or an industrial machine for making ice on a
large scale.
16 Autoclave/sterilizer - is a device used to sterilize equipment and supplies by
subjecting them to high pressure saturated steam at
121 °C for around 15–20 minutes depending on the size of
the load and the contents.
17 Binder -It is suitable for all sensitive incubation applications and
ensures optimal cell growth.
18 Dual action shaker -is a device that have Orbital or reciprocating shaking action over
a wide speed range (10 to 300 rpm) offering an ideal solution for
your mixing needs
19 Beakers -is a simple container for stirring, mixing and heating
liquids commonly used in many laboratories.
20 Cylinders/Graduated -is a piece of laboratory equipment used to measure the
cylinders volume of a liquid. Graduated cylinders are generally more
accurate and precise than laboratory flasks and beakers.
21 Erlenmeyer flask -The tapered sides and narrow neck of this flask allow the
contents of the flask to be mixed by swirling, without risk
of spillage, making them suitable for titrations. Such
features similarly make the flask suitable for boiling
liquids. Hot vapors condense on the upper section of the
Erlenmeyer flask, reducing solvent loss. Erlenmeyer flasks'
narrow necks can also support filter funnels.
22 Syringe -is a simple pump consisting of a plunger that fits tightly in
a tube. The plunger can be pulled and pushed along inside
a cylindrical tube (called a barrel), allowing the syringe to
take in and expel a liquid or gas through an orifice at the
open end of the tube.
23 Thermometers -is a device that measures temperature or temperature
gradient using a variety of different principles.