ABSTRAK

Judul : UJI SITOTOKSIK EKSTRAK ETANOL DAUN KERSEN

(Muntingia calabura L) TERHADAP SEL KANKER WiDr
SECARA IN VITRO

Kersen (Muntingia calabura L.) merupakan salah satu tumbuhan

yang diduga berpotensi sebagai antikanker. Penelitian ini bertujuan
untuk mengetahui aktivitas sitotoksik secara in vitro ekstrak etanol daun
kersen terhadap sel kanker WiDr. Ekstraksi daun kersen dilakukan
dengan maserasi menggunakan pelarut etanol 96%. Pengujian aktivitas
sitotoksik dilakukan dengan metode MTT ([3-(4,5-dimetiltiazol-2-il)-2,5
difeniltetrazolium bromida]). Hasil pengujian dibaca dengan ELISA reader
pada panjang gelombang 595 nm. Pengolahan data untuk penentuan nilai
IC50 dari sel diperoleh dengan menentukan regresi linear antara log
konsentrasi sampel uji dan persen kematian selanjutnya dianalisis probit
menggunakan Microsoft Excel 2010. Hasil uji sitotoksik menunjukkan
ekstrak etanol daun kersen memiliki aktivitas sitotoksik ditunjukkan
dengan nilai IC50 88, 31 μg/ml.

Kata Kunci: Sitotoksik, Daun Kersen (Muntingia calabura L), Sel

kanker WiDr, MTT
 ABSTRACT

Title : SITOTOXIC TEST OF KERSEN LEAVES ETHANOL

EXTRACTS (Muntingia calabura L.) ON WiDr CANCER
CELL IN VITRO

Kersen (Muntingia calabura L.) is one of the plants that is thought to

have potential as an anticancer. This research was aimed to determine the
in vitro cytotoxic activity of ethanol extract of kersen leaves on WiDr
cancer cells. Extract of kersen leaves was done by maceration using 96%
ethanol. Testing of cytotoxic activity was carried out by the MTT method
([3- (4,5-dimethylthiazol-2-il) -2,5difeniltetrazolium bromide]). The results
were read by an ELISA reader at a wavelength of 595 nm. Data
processing for determination of IC50 values from cells is obtained by
determining the linear regression between the log concentration of the test
sample and percent of mortality and then probit analysis using Microsoft
Excel 2010. The cytotoxic results were shown an ethanol extract of kersen
leaves having cytotoxic activity indicated by IC50 88,31 μg/mL.

Keywords: Cytotoxic, Kersen Leaves (Muntingia calabura L), WiDr

cancer cells, MTT