Objetivo: Optimizar la actividad enzimática (respuesta) de la inulinasa, utilizando

temperaturas de 30 a 70 °C y pH entre 3 y 7

Codificados Reales Actividad

Ensayos Temperatura Temperatura
pH pH (U/ml) AE - M codi
(°C) (°C)
1 -1 -1 3.6 36 272 231.33
2 1 -1 6.4 36 83 101.43
3 -1 1 3.6 64 457 456.22
4 1 1 6.4 64 16 74.31
5 -1.41 0 3 50 360 393.04
6 1.41 0 7 50 83 32.21
7 0 -1.41 5 30 132 151.41
8 0 1.41 5 70 328 290.84
9 0 0 5 50 396 393.05
10 0 0 5 50 412 393.05
11 0 0 5 50 393 393.05
12 0 0 5 50 371 393.05
¿Qué valores de T y pH producen la mayor actividad enzimática?
 codificado
AE - M Real

232.94 Regressn Coeff. Std.Err. t(6) p

105.10 Mean/Interc. 393.053 22.05741 17.81953 0.000002
457.44 (1)PH C(L) -127.952 15.62034 -8.19137 0.000178
77.60 PH C(Q) -90.753 17.51092 -5.18268 0.002049
391.77 (2)T C(L) 49.442 15.62034 3.16524 0.019436
29.14 T C(Q) -86.478 17.51092 -4.93852 0.002608
148.60 1L by 2L -63.000 22.05761 -2.85616 0.028947
289.31
392.77
392.77
392.77
392.77

ELIMINANDO EL COEFICIENTE MEN

ELIMINANDO EL COEFICIENTTE MÁ
 real

-95.% Cnf.Limt
+95.% Cnf.Limt Regressn Coeff. Std.Err. t(6)
339.080 447.0253 Mean/Interc. -2359.24 447.9695 -5.26653
-166.173 -89.7303 (1)PH (L) 525.85 107.7538 4.88012
-133.601 -47.9058 PH (Q) -45.58 9.0186 -5.05392
11.220 87.6637 (2)T (L) 63.04 10.7754 5.85070
-129.326 -43.6303 T (Q) -0.43 0.0902 -4.81829
-116.973 -9.0270 1L by 2L -3.21 1.1571 -2.77779

SS df MS
(1)PH (L) 130182.8 1 130182.8
PH (Q) 52553.2 1 52553.2
(2)T (L) 19600.5 1 19600.5
T (Q) 47767.1 1 47767.1
1L by 2L 15876.0 1 15876.0
Error 12345.1 6 2057.5
Total SS 260974.3 11

SS df MS
modelo 248629.2 5.0 49725.8351
residuos 12345.1 6.0 2057.5124
total 260974.3 11.0

r2 95.27%

SS df MS
modelo 232753.2 4.0 58188.2939
LIMINANDO EL COEFICIENTE MENOS SIGNIFICATIVO residuos 28221.1 7.0 4031.58206
total 260974.3 11.0

r2 89.19%
 SS df MS
modelo 229028.7 4.0 57257.1702
residuos 31945.6 7.0 4563.65277
LIMINANDO EL COEFICIENTTE MÁS SIGNIFICATIVO total 260974.3 11.0

r2 87.76%
 p -95.% Cnf.Limt
+95.% Cnf.Limt
0.001890 -3455.38 -1263.10
0.002766 262.19 789.52
0.002325 -67.65 -23.51
0.001100 36.68 89.41
0.002945 -0.66 -0.21
0.032091 -6.05 -0.38

F p
63.27195 0.000210
25.54209 0.002325
9.52631 0.021485
23.21596 0.002945
7.71611 0.032091 F
2 fa c to r s , 1 B

75

F p 70
24.1679394 0.000658022
65

60

55

50
T

F p
45
14.4331166 0.00171385

40

35

30

25
2 .5 3 .0 3 .5 4 .0
 35

30

F p 25
2 .5 3 .0 3 .5 4 .0
12.5463468 0.002612806
 F it t e d S u r f a c e ; V a r ia b le : A E
2 f a c t o r s , 1 B lo c k s , 1 2 R u n s ; M S R e s id u a l= 2 0 5 7 . 5 1 2
D V: AE

400
200
0
-2 0 0
.5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 6 .0 6 .5 7 .0 7 .5
-4 0 0
 400
200
0
-2 0 0
.5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 6 .0 6 .5 7 .0 7 .5
-4 0 0
PH