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metabolism
Five women who developed hypertension during pregnancy received metoprolol, 10 mg iv; 3 days later
they received metoprolol, 100 mg by mouth. Blood and urine samples were collected after each dose. The
same procedure was repeated 3 to 6 months after delivery. The apparent oral clearance of metoprolol
during pregnancy exceeded that after pregnancy by a factor of 2 to 13. As a result, after oral dosing the
peak plasma concentrations during pregnancy were only 12% to 55% those after delivery, and the plasma
AVCs were reduced to the same extent. Oral bioavailability increased by a factor of 1.3 to 3.7 after
pregnancy. Systemic clearance after pregnancy was 26% to 97% that during pregnancy, but this difference
was not significant. Metoprolol plasma protein binding was the same on both study occasions. Our data
cannot be explained by a change in gastrointestinal absorption, because the urinary recovery of metoprolol
and its metabolites was slightly higher during pregnancy. It is concluded that the greater metoprolol
clearance during pregnancy results from increased hepatic metabolism of the drug. (CLIN PHARMACOL
THER 37:688-692, 1985.)
Stellan Hogstedt, M.D., Bo Lindberg, M.D., Ph.D., Dun Ren Peng, M.D.,
Carl-Gunnar Regardh, Phann.D., and Anders Rane, M.D., Ph.D.
Viisteras, Uppsala, Molndal, and Huddinge, Sweden
During the last few years, cardioselective [3-blocking who are not pregnant, it is completely absorbed after
drugs have largely replaced diuretics in the treatment oral dosing."
of hypertension during pregnancy. II One of the most We studied metoprolol kinetics in five women during
widely used drugs in this category is metoprolol, a and after pregnancy. The study was undertaken to elu-
cardioselective [31-adrenoceptor blocker." The antihy- cidate the mechanism behind the increased oral clear-
pertensive therapy is often reinforced with hydralazine. ance of metoprolol during pregnancy.
This combination has proved effective in most cases of
pregnancy-induced hypertension and, apart from the METHODS
known side effects of these drugs, the treatment appears Our subjects were five women 20 to 36 years old
safe for both mother and fetus. 8 Despite the extensive who were studied the first time while in the third trimes-
use of metoprolol, very little is known about its kinetics ter of pregnancy. The second study was performed be-
during pregnancy. Pregnancy is associated with an in- tween 3 to 6 months after delivery. Clinical features of
creased clearance of several drugs that are oxidized or the subjects are listed in Table I. Four of them developed
excreted directly by the kidneys. 1,9,10,13 We have re- hypertension before the 20th week of pregnancy, while
ported a marked increase in the apparent oral clearance the fifth (no. 5) developed a typical preeclampsia with
of metoprolol during pregnancy." Metoprolol is almost profound albuminuria (1.2 gm/24 hr the day before
entirely metabolized by the liver and, at least in subjects delivery) in the 36th week of pregnancy. Other than
hypertension and albuminuria, subjects had no com-
From the Department of Obstetrics and Gynecology, Vasteras Hos- plications during pregnancy. None was treated with any
pital, Vasteras; Department of Obstetrics and Gynecology, Uni-
drugs during pregnancy or during the postpartum study.
versity Hospital, Uppsala; Department of Pharmacokinetics and
Drug Metabolism, AB Hassle, Molndal; and Department of Clin-
Subject 2 developed rheumatoid arthritis after delivery
ical Pharmacology at the Karolinska Institute, Huddinge Hospital, but was not taking any drug during the second study.
Huddinge. Four subjects had a spontaneous vaginal delivery; the
Supported by grants from the Swedish Medical Research Council subject with preeclampsia underwent Caesarean sec-
(No. 04X-04496), The Expressen Prenatal Research Foundation, tion. All infants were healthy and had normal birth-
and the Karolinska Institute.
Received for publication Nov. 8, 1984; accepted Feb. 14, 1985. weights according to Swedish reference values.
Reprint requests to: Stellan Hogstedt, M.D., Department of Obstet- During the first day of the study, 10 mg metoprolol
rics and Gynecology, Vasteras Hospital, 72189 Vasteras, Sweden. was injected intravenously into an arm vein in the mom-
688
VOLUME 37
NUMBER 6 Pregnancy-induced metoprolol metabolism 689
Subject No.
1 2 3 4 5
No. of previous pregnancies/deliveries 0/0 0/0 0/0 0/0 I/O
Smoking (cigarettes per day) 0 <10 0 0 0
Body weight (kg) on both study occasions 80/68 81/71.5 95.5/88 78.5/69.5 74/63
Week in pregnancy/after delivery (on study occasions) 38/25 35/24 35112 37112 38114
Delivery week (completed) 38 36 39 39 38
Birthweight (gm) and sex 3500(F) 2540(M) 2880(M) 3150(M) 3270(M)
First appearance of hypertension (wk of pregnancy) 14 18 11 20 36
Maximum blood pressure during pregnancy (mm Hg) 150/100 150/100 160/105 1501110 1551120
F = Female; M = male.
ing after an overnight fast. Two hours after drug dosing Table II. Metoprolol kinetics after oral and
the subject ate a standardized breakfast. Blood samples intravenous doses in five women during and
were drawn through an indwelling catheter before, 5, after pregnancy
15, 30, and 45 minutes, and 1, 1.5, 2, 3, 4, 6, 8, and
During After
12 hours after dosing. Three days later, when virtually pregnancy delivery
the entire intravenous dose had been eliminated from
the body, 15 the subject received an oral dose of 100 mg Oral (100 mg)
tY2 (hr) 3.42 ± 0.80 3.42 ± 0.31
metoprolol. The same procedure as before was fol-
AUC (nmol . hr/L) 998 ± 517 3562 ± 809*
lowed, except that blood samples were drawn before, CIa (Umin) 9.56 ± 2.70 1.71 ± 0.39*
20 and 30 minutes, and 1, 1.5, 3, 4.5, 6, 8, and 12 CIa (Umin . kg) 0.118 ± 0.034 0.024 ± 0.006*
hours after dosing. All blood samples were centrifuged F 0.21 ± 0.06 0.42 ± 0.05t
within 10 to 60 minutes of sampling and the plasma Intravenous (10 mg)
tY2 (hr) 5.38 ± 1.36 5.36 ± 1.18
was frozen at - 20° C until analyzed. A small portion
Vd (Llkg) 6.87 ± 1.32 3.85 ± 0.43
of the blank plasma (collected before metoprolol dos- C!;, (Umin) 1.38 ± 0.26 0.65 ± 0.08
ing) was saved for the assay of the metoprolol plasma C!;, (Llmin . kg) 0.017 ± 0.003 0.009 ± 0.001
protein binding. Plasma protein bind- 9.18 ± 1.70 10.86 ± 1.06
Urine was collected over 24-hour intervals for 3 days ing (%)
after both doses of metoprolol. The volume of each Data are X ± SE.
portion was measured and 30 ml was frozen at F = Bioavailability.
*p < 0.05, tP < 0.01 compared with value during pregnancy.
- 20° C until analyzed.
The concentration of metoprolol in plasma was
determined by gas chromatography." Metoprolol and acid (H 104/83) is also determinable by this method,
the metabolites a-hydroxymetoprolol (H 119/66) and but it is not fully optimized and, therefore, we did not
O-demethylmetoprolol (H 105/22) were quantified in calculate the amount of this metabolite excreted in the
urine by gas chromatography-mass spectrometry (Fig. urine. Metoprolol plasma protein binding was deter-
1).3 The lowest measurable concentration at a standard mined at 37° C by an equilibrium dialyzing cell system
deviation of 10% is 1 nmollL for all three substances. with a cellulose membrane."
The acid metabolite (H 117/04) was analyzed in urine The disposition kinetics of intravenous metoprolol
according to a recently published gas chromatographic were evaluated by a three-compartment model with non-
method" based on a cyclization of the aminoalcohol side linear regression analysis. The program ELSFIT* was
chain with phosgene at alkaline pH. After completion used in the calculations. Systemic clearance (CI;J was
of the reaction, the oxazolidineone derivative is ex- determined by the ratio: Cl., = Dose.i/ AUC~.iV' in
tracted to an organic phase at acid pH. Before capillary which AUCoc,iv is the plasma AUC obtained by inte-
column gas chromatography with flame ionization de- grating the computer-derived triexponential equation.
tection, the remaining carboxylic group is trimethyl- The elimination rate constant of the terminal phase of
silylated. The minimum determinable concentration of
H 117/04 in urine was about 20 urnol/L. The hydroxy *Beal and Shiner, University of California at San Francisco.
CLIN PHARMACOL THER
690 Hagstedt et al. JUNE 1985
~:"OCH
oral and intravenous dosing, oral bioavailability was
lower during (0.08 to 0.43) than after (0.30 to 0.56)
-> 223 pregnancy, with a mean difference of 260% (Table 11).
METOPROLOL H 104/83 Metoprolol plasma protein binding during and after
pregnancy did not differ, but the binding was only 3%
in subject 3 during pregnancy and II % after parturition
"" OH CH3
(Table II).
9A
"'\. O'CH 'CH'CH 'NH'CH
2 2 I
The total urinary recoveries of metoprolol and its
CH3
metabolites during the first 24 hours after the oral dose
r H ' CH ' OCH
2 3
were higher during pregnancy than after delivery (ex-
OH cept in subject 3 [Table III]). After the intravenous dose,
H 119/66 urinary metabolite concentrations were probably over-
estimated because of low urine metabolite concentra-
Fig. 1. Major metabolic pathways of metoprolol: a-hydroxy- tions and cochromatographing peaks. Therefore, these
metoprolol (H 119/66), O-demethylmetoprolol (H 105/22), results are not reported.
an acid metabolite (H 117/04), and a hydroxy acid metabolite
(H 104/83). DISCUSSION
We have reported' and now confirm that the me-
toprolol apparent CIa is more than four times higher
the plasma concentration-time curve of the oral dose during than after pregnancy. Our study was designed
(13) was calculated by linear regression analysis of the to explain the mechanism for this difference. Metopro-
log plasma concentration-time curve. The terminal tYz 101 is completely absorbed in man. 15 In all but one
was determined as: tYz = 0.693/13. The plasma AUC subject the urinary recovery of the oral dose was higher
of the oral dose (AUCor• l ) was calculated by the trap- during pregnancy than after delivery. The reason for
ezoidal rule. The residual area was calculated as C/I3, this is not clear but might be a consequence of either
where C is the plasma concentration at the last sampling more extensive formation of the metabolites, mainly
time. The apparent oral clearance (CIa) was calculated the carboxylic acid, or more rapid excretion of the me-
as: CIa = Dose/ AUCor• l . tabolites by the kidneys during pregnancy. On the basis
Results are presented as X ± SE. Statistical analyses of our findings, we believe that the absorption is, if
were with Student's paired t test. anything, higher during pregnancy than in the non-
pregnant state. These findings would thus rule out im-
RESULTS paired absorption as the reason for the large difference
No subject reported any adverse effects on any of in apparent CIa and the reduction in systemic avail-
the four study occasions. After the intravenous dose, ability.
the plasma tYzs were of the same order during and after Several drugs, such as phenytoin and salicylates,
pregnancy. Metoprolol Cl., fell after delivery to 26% have lowered plasma protein binding during preg-
to 97% (X ± .SE = 55.0% ± 12.6%) of the value nancy.v" Changes in the plasma protein binding of
during pregnancy (calculated on the non-weight-related metoprolol cannot explain our findings, because we
clearance values). The apparent volume of distribution found this to be very low. Therefore, alterations in this
(Vd) was higher during pregnancy, although not sig- parameter will not affect metoprolol kinetics. Further-
nificantly so (Table II). more, there was no clear difference in binding during
The peak plasma concentration was reached 0.5 to and after pregnancy.
VOLUME 37
NUMBER 6 Pregnancy-induced metoprolol metabolism 691
".t.""
-------
2 3 .. 5 • 7 • (t 10 11 12 hr 2 3 .. 5 e 7 8 , 10 11 12 h' 1 2 3 .. 5 IS 7 II , 10 11 12 hr
_ o r a l dose pregnant
oral dose not pregnant
0- - - -0
_ i . v . dose pregnant
6 - - - - 6 i.v. dose not pregnant
2 3 .. 5 • 1 • • 10 11 12 h. 2 3 .. 5 e 7 8 , 10 11 12 hr
Fig. 2. Metoprolol plasma concentrations after single intravenous (10 mg) and oral (100 mg) doses
in five women with hypertension in the third trimester of pregnancy and after delivery.
Table III. Urinary excretion of metoprolol and its metabolites during and after pregnancy over the first 24 hours
after an oral dose of 100 mg (292 umol) metoprolol