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Ministry of Agriculture
Department of Science & Technology
Council of Scientific & lnudstrial Research
Ministry of Science &Technology
lndian Association for the Advancement of Veterinary Research

'

., .1.:.-i,
 lfogicaf investigations on' pubertal
sexual activity , rateof 46.15%intreated animars, was
seen, whereastreatnrent
;iff*:[];T::::|fijX':f
y'lls eated on fotrr different out in;{T::'}:$lf
sixteen .;;;
u,"*r was main,y with GnRH (4, ,7.,.)
r"ctntcat parameters were carried
Lsyg.'r:t:r
;,,il"[#ffi;fitrffiUn:,irr!*r9;#,il#ll3?
"""*tttt""ll,ppt.,n.nt, il;;;; iodide (5.88%) and an overa, pregnancy
rate of
neated;il"h ,",,
*:"':[iryH;j:T',""'i.Jr:*i*l**:f
respectiverv'
or'*p"'in,.n,:,+';'$
respectively' Afternine
*,.^
ijJ*i"
After nine monthes or.xp"rinr.nrr, *"rr^-
ro,na- n,e overa, pi.g,,on.y
11r:ate9 animals of group-lll
was 27.27o/o. tt"can be
observations were recorded
i
at about I ttt""'rtt or age.-it
::fi1#ffiT*T;il?*i,tfiJl,liifiXil;?lhml:
.
manifestation ofearlv pubertal ir-.i'Jrrur, more common in the right
animals fed cotton;;d.nteal
,ign, *ur,,r;*in,u,n (isz") "
i,, ,or"|."uuf.nt, the diseas"-ruy ,.rolue ovary, tutear cysts are
u"J"y"it't. onJ iir"i ."lrri"rr, used treatments iictuding
spontanously and
"'ia
::'ilT,::iJ:f:T::"i"""1ffi;TfJJj,toJ'
'"'otot
u'tt"' in tt," J"-Ju,,"" are
the ord rreatnrent
effe*ive iot,.uiing tr," disease. rhe
disease is
of
without.;"'i;.;;li;ne.enc-e
significant difference was evident
ilil;-,",*Jii,*iJfj ifi,H:H;:ns '.noin'fl.oJj rin., .*r,iiiti"g i,,.,iio.y
in ti" r.iu, biochemical
paranreters studied due-to
dietary varitions, the values
of FERTIL_ISATTONFAILUREDURING','ERovUL{T'.N:
,-#fi?,::'iji;"J:T,il:,ifn]i',fji:ffi.1'"*"
meal
AMAroRcoNsrnaixii*E-nrBRyoREcovER'
arrd barley feeding. oieratl, G'N. Purohit, S.s. sharma, Matresh
the result ,noi.'oJtltted
calves fedbarery in-"oriinution
with cottonseed mllat
the bull vyas nndK.A- Gupta
Datt, Aminudeen, firmal
to be superiot;;;;;; paranreters Dent.ofvety.obsietricsandGynaecology,collegeofVety.&
srudy. -" rs"urrlrurr dssesseo ,nellaPPeared
assessed In the Bresent
Animats"i.1,".,el-k;";i"+i'di'inojor*,onl

ANOVERVIEW OF CYSTIC Superovulation was induced in

OVARIAN DISEASEIN
RATHICATTLE
G.N.Purohit, B.KJoshi. B.L.
Bishnoi, A.KlGupta, RK.Joshi,
S.K.Vy:rsand KA.Gupta
Department of Veterinarv
Obstetrics and Cynaecology,
of Veterinary and Animal College
Science, ,,U".r"i'*E"rin.".
,{1.3v3rvierv ofcysric ovarian
disease (COD) observed in
cattle from Jan. | 991 to
March l99g unae, ru.at and urban
Rarhi
is presenred.Srudy is comparted i;;;;;;'gr"rps.
areas
Group I
conrprised 7g infertility
' camps organised-irr the rural
Cases presented for treatment
at the alongwith studies on
serum biochemistry formed theit
theCroup1| n]rA r^", investigated
at th.e College dairy. farm
biochernistry fonned the
alongwi,i ,r"ii,
on their serunr
COD in Rathi cows in rural
C.orpift- ii"
f incidence of
"""rrf
una ,rOun ur.or'**
0.87olo respectively. g0Vo Z. l2%o and
of tfr" unirnui, *"r. S-A years
and in their 0-4th laoatiox. of age
ffr".yrt, ,r"rri"."r.O on the right
ovary 56.03%o. reft ovary 2g.72%ind
onuottr Ju*ies in 17.24o/o
of the totalcases. rhe fregurnry;;;;;il;;;,s
and muf tiple cysts tg.gzo/o:.ffr..yst,
was 80.t7%
*Jr. #u,f.,
in size and
differentiation from normal
,orioru tutea neeOed thorough
rrregular
Nymphomania with frequent
lT:*,"0,intervals, ,ruu, u, regular or
or constant estius was the predorninant
clinical sign encountered in
56.300/o of ifr"'"*., whereas
anoestrus rvas found in 43.96o/oof
the cases. Amongst other
ass::'.aled
:
iCns. petvic relaxation, ctitoral
frii"rt opf,y, uterine
an d cf i n al path o lo gv were s een in
33.6296, ii.ZZ y, ana Zt.q Cy,
i c
9 Rathi and its cqossbred heifers
usin! two treatments scheduhr.
cyclic and Z acyclicheifers that
G;;;i"J cornprisc<l of2
were inlecteawittr pMSG (on
day 8 to the cycle), the acyclic
treifers berng pretreated with a
progestagen inrplant. Croup
B (n=5) inctujed acyclic hcifers
pretreated- wirh a progestagen
implant followed by FSH (Super-
ov) injections in equal doies on
il"y Z,S 9 inplant at
l2hrly intervals. Exce ""J "fthe
s h owed q""d ; ;;;i:ffi il "::iT,111,?f;
t[,i:H$',i
implant had no"; effect on the
estrual intensity during
superovulation. The mean ovulatory
areas. and l2.6al.tS in group A and
B
response was g.25x23.32
,.rp..iiu.ly. ihe difference
betrveen the groups was non_significant
and ,h"-."un enrbryo
recovery was 75J3+10.35 and
respectively, however the number
71.5g+7.53% in groups A and
B
donor.was very poor in both
of embryos-recovercd per
ttre groups';;;;." nunrber
unfertilized ova were recovereO. of
Onty i.nirnul, oftn.4 rvhich
responded in group A yietded
5 biastocysts-and 4 rrrorura
one animat yielded 8 unfertitiJ'olu
:l::=t
4(415) animats which responded iuool. .rr,r,"
to treatmen, in group B yielded
39 UFO and 5 degenerated embryos
e"iir",, qhorving a
medi unr s u perovu latory response had ",rly.
.quut n urn U". of ,nl fr.yi,
and.unfertitised ova, *h"..u, th"r;
excellent response had nrore numberof
;i;;;; I pcio,r and
UFO.
PRECNANCY RATES IN BUFFAI..OES
INSEMINATED ON
THE BASIS OF VAGINAL ETECTNTCiI
(vER)
Nri,Sr^NCB

of the cases. Bioch-emical KA.Gupta and G.N.purohit

in't]i"looo serum of
nonnal and cystic cows "rti,nution.
ofgroup-lll Dept.of Vety.Obstetrics and_Cynaecology,
revealedrignifi.*tfy higt ., Coltege of Vety.&
values of chloride and anirnal Science, Bikarrer-33400'i
signincanuy to;.;-";i;;, of inorganic tn"j^tfrLt
phosphorus 1p<0.05)
in cystic cows. Treatnrent in the group_ Vaginal electrical resistance
I(rural area) consisted rnainly was recorded in lZ7 butlaloes
of.nu.t*tion (5641 %) followed beonging to a research farm (n-_4Ol
by administrarion of potassi-um f*.o in=isiano inauced
and PGF2 alpha injectio 19Oia. 1io.s) ",,")i, ru1rr.nor"1 estrus group (n=r2) at the time
of insemnation, using a
ns (5.12%)*a no oV"ruf l pregnancy commercially available vaginal
resistance probe (Heat detector

37
 ner, Cermany) and was fognd to vary between
for all the groups. The conception rates to single
on were highest in allthe groups when the VER was
26-30 Ohms and were 80.75o/oand 1007o for the three
whereas, the conception rates were 62.50/o,55.17%o and
wh*:r-: ths VER was between 3 I -35 Ohms. At slightly higher
VER of 36-40 Ohms the conception rates were barely low of
33.33%, 19.23% and 0o indicating therbj that.inseminating
buffaloes at a low VER of 26-30 Ohms clearly increase the
conception rates. Farmers at the A.l. centres should be advised
to bring their animals for insernination at the correct time.
preferrably l2 hours after estrual onset. Conceptionrates of
farm buffaloes were better reflecting better mangement and
estrus detection.
EFFICIENCY OF VAGINAL ELECTRICAL RESISTANCE
(vER) FOR |NSEMTNATTNG CATTLE
G.N.Purohit and K.A.Gupta
Department ofVeterinary Obstetrics and Gynaecology, College
ofVeterinary and animal Science, Bikaner-334001 (Rajasthan)
Vaginal electrical resistance (VER) and ovarian findings were
recorded in 224 cows that were inseminated using frozen semen.
28 cows were also insenrinated without recording VER. It was
noticed that there was a rvide difference between the conception
rates with fi rst insemination wh iclt were 92.5%o, 58.46%, | 6.27o/o.
5.55% and 42.85%o respectively when the cows were
inseminated at a VER between 26-30 Ohrns (Group I), 3l-35
Ohms (Group ll),36-40 Ohms (Group III),41 Ohms and above
(Group lV) and without VER recording (Group V). Low VER
(Croup I and ll) closely coroborated with ovarian finding of a
mature follicle whereas with high VER (Group II and til)
ovulation had already taken place. It was conclucled that
inseminating cows at a low VER is distinctly advanrageous ro
improve tlre conception rate in cows.
VINBLASTIN-PODOPHYLOTOXIN A GOOD M ITOTIC
AGENT FOR EMBRYONIC CHROMOSOME PREPARAT-
ION
Mahesh Datt and Arjava Sharma
Department of Obstetrics and Cynaecology, College of
Veterinary & Animal Science, Bikaner-334001 (Rajasthan)
A combination of vinblastin-podophylotoxin was used for
cytogenetic evaluation of in vivo produced mouse embryo.
Cytogenetic evaluation of in yiyo produced mouse embryo
was made by processing 45,110,214 and 52 enrbryos of <g,g-
16,>16-32 md>32 cell stage vyhich had 60,285,660 and 241 cells
in mitosis respectively. Overall there were 421 ernbryos having
i:: 1246 cells in mitosis. Mitotic index ofembryos of four categories
were recorded as 0.9,0.22,0.13 and 0.13 respectivety.
i Concentration of mitotic agent has a significant effect on mitotic
index of embryonic cells.
I
::,
EFFECT OF PROG ESTERONO.SUPPLEMENTATION ON
PREGNANCY RATE IN REPEAT BREEDERCOWS
g reasonably high (350-a00 pprn). Similarly rhey can satrly be
td S.K. Srivastava and S.D. Kharche
9l
.:;
::;_
38
::j
A.l. Laboratory, Livestock Production Management, lndian
Veterinary Research Institut e,lzatnagar - 243122(U .P .\ -
Effect ofprogesterone ofpregnancy rate was studied in forty
eight repeat breeder crssbred cows. Experiment was conducted
on cbws, maintained at Livestock Production Managernent
farm, IVRI, Izatnagar which could not conceive even after 7 to
8 inseminations. 250 mg porgesterone (Duraprogen, Unichem
Lab. Ltd.) was administered intramuscularly in 23 cows on days
5th, l2th and l9th post A.I.Remaining 25 cows were treated
with normal saline as control. All the cows were examined for
pregnancy per rectally at 45 to 60 days after inseinination. Our
of 23 cows in treatment group 9 becanre pregnant after first
service with a conception rate of 39.13o/o. 3 cows became
pregnant after second service with conception rate 21 .42%.
Remaining two cnceived after after third and one after fourth
service. All 23 cows conceived on a total of 2.2 services with
overall conception rate 65.21o/o. First service conception rate
in control was only 8.00%o and all 25 cows conceived on a total
of4.08 services with a conception rate of 48o/o. The difference
in first service conception rate and overall conception rate
between treated and control group was highly significant
(P<0.01 ). Thus this experiment show significant improvernent
in conception rate ofrepeat breeder cows after progesterone
supplementation.
PHYSIOLOGY, BIOCIIEMISTRY.
NUTRITION
IMPORTANCE OF SMALL RUMINANT PRODUCTION IN
ARID AND SEMI-ARID ECOI]OG IES
J.P.Mittnl. A.K.Dang and S.lM.KNaqvi
Division of Animal Physiolgy, Central Sheep and Woot
Research Institute, Avikanagar (via Jaipur), Rajasthan 304501
In arid and semi-arid regions where shrubs and coarse
vegetation abound, but hardly consumed by large run.lillants,
the small runtinants aie able 1o thrive very weel. These rcgions
are endowed with some of the best breeds of sheep and goats.
Small ruminants have been incriminated as creatures ofdcsertic
conditions which is not true. Studies conducted on this aspect
clearly revealed that controlled and judicious grazirrg on
deserted land over the years could eventually convert it into
ferti le land. I nspite of severe env ironmental hazards and3 0%o
40Yo annual slaughter rate numbers of small ruminanls are
-
constanly increasing which speaks of their hardiness''and
adapability to harsh clirnatic conditions ofthese regions. 'Ihese
anirnals often walk scores of kilometers ufder blazing sun in
search of food and water, yet providing animal protein in the
form of meat and milk alongwith byproducts to the people ot
these difficult regi,ons. Results of longterm exper.irnents
indicated that their tolerance to feed, fodder and water. scarcity
is higher ihan large ruminants. They can be maintaincd in
productive stage on 3 watering per week atleast during.lean
period . Their tlerance to underground saline water is
manged in productive stage on waters containing Nitratc upto