Ghasemi Pirbalouti et al., Journal of Herbal Drugs, No 1.

2010

Journal of Herbal Drugs

journal homepage: www.jhd.iaushk.ac.ir

The effect of some of the Iranian medicinal plants on Brucella Abortus on In-
vitro and In-vivo
Abdollah Ghasemi Pirbalouti1, Mohammad Reza Ghasemi2, Hasan Momtaz3, Ahmad Reza
Golparvar4, Behzad Hamedi5, Lohraseb Shahgholian6
1- Assistant Professor, Research Center of Medicinal Plants and Ethnoveterinary, Islamic Azad University, Shahrekord Branch,
Shahrekord, Iran. POBox: 166. (Corresponding author: ghasemi955@yahoo.com)
2- DVM, Veterinary Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3- Assistant Professor, Department of Microbiology, Veterinary Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
4- Assistant Professor, Department of Agronomy, Agriculture Faculty, Islamic Azad University, Khorasgan Branch, Isfahan Iran
5-Bc. Research Center of Medicinal Plants and Ethnoveterinary, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
6- Assistant Professor, Department of Physiology, Veterinary Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

ARTICLE INFO ABSTRACT

Introduction & Aim: The ethanol and aqueous extracts of Alhagi
Type: Research Original
camelorum Fisch. (Fabaceae) whole plant, Scrophularia desertii Del.
Topic: Pharmacology, Ethno-veterinary (Scrophulariaceae) whole plant and ethanol extracts of Echinophora
Received 10 January 2010 platyloba DC. (Apiaceae) whole plant, Teucrium polium L.
(Lamiaceae) whole plant and Berberis integerrima Bunge root brake
Accepted 7 March 2010
were investigated for anti-Brucella in-vitro and in-vivo conditions.
Experimental: Clinical isolated of Brucella abortus was studied using
Key words: the disk diffusion method. Brucellosis was induced by repeated
 Medicinal plants injection of Brucella abortus on Balb/C mice. In Balb/C model, we
 Brucella abortus evaluated factors included primary weight/secondary weight ratio
(W1/W2), spleen weight, spleen weight/body weight ratio and
 in-vitro antibody titer with Wright method.
 Balb/C Results & Discussion: The results of disk diffusion approach that the
 Brucellosis
extracts from different plant species studied showed antibacterial
activity against B. abortus, with the diameters of zone of inhibition
ranging between 1 and 27 mm. Of the plants studied, the most active
extracts at the concentration 500 µg/ml were those obtained from
aqueous extracts of A. camelorum whole plant and showed strong
inhibitory effects (zone of inhibition ≥15 mm) but ethanol extract of S.
desertii at the concentration 125 µg/ml showed lowest of inhabitation
effect. The bacteria in the study were sensitive to some of antibiotics,
erythromycin and streptomycin being the most sensitive (inhibition
zone values of 21 and 23 mm, respectively), while was resistant to
some of the plant extracts at the concentration 62.5 µg/ml. The best
results for W1/W2 were obtained from extract-treated by ethanol
extract of B. integrrima root in dose of 400 mg/kg/day and following
treated by erythromycin in dose of 400 mg/kg/day. The lowest of
Spleen weight were obtained from aqueous extract of A. camelorum
and following treated by erythromycin. The best results for Spleen
W/Body were obtained from extract-treated by aqueous extract of A.
camelorum and following treated by erythromycin in dose of 200
mg/kg/day. Also, the best results for antibody titer with Wright method
were obtained from ethanol extract B. integrrima root brake. However,
the results of mean of comparison with Duncan's test for antibody titer
with Wright method showed no significant difference.