Course Syllabus Derek Boyer

Biology 210 dboyer@occ.cccd.edu

Spring 2011 Office Phone: (714) 432-5825
Office: Bio Sc. 212A Hrs: M/W 1:00-2:00pm: Bio Sc. 207 (Lab)
T/Th 11:15-12:15pm: Bio Sc. 212A

Course Description: Biol 210 is a 5 unit college transferable course that introduces you to the
microbial world…a world of viruses, bacteria, molds, yeasts, protozoa, algae, and helminths.
Emphasis will be placed on microbial structure, metabolic activities, microbial genetics, host-
parasite relationships, host resistance, and the control of microbes. Lab activities will include
isolation and cultivation of bacteria, studies of bacterial isolates, understanding bacterial population
dynamics, control of microbial growth, mycology, parasitology, and virology.

Required Textbook: Microbiology: An Introduction 10th Ed by Tortora, Funke, and Case

Required Lab Manual: Laboratory Theory and Application, 2nd or 3rd Ed. by Leboffe and Pierce

Derek Boyer’s webpage: http://occonline.occ.cccd.edu/online/dboyer has links to supplementary

materials and a copy of this course syllabus.

Student Learning Outcomes:

1. Demonstrate appropriate laboratory skills and techniques related to the isolation, staining,
identification, assessment of metabolism, and control of microorganisms.
2. Evaluate the physical and chemical methods used in the control of microorganisms and apply
this understanding to the prevention and control of infectious diseases when given a scenario.
3. Discuss and diagram the role of the immune system in maintaining homeostasis, challenging
infections, and fighting cancer.

Grading: Your course grade is based on a percent of accumulated points. These points are
acquired by examinations, quizzes, lab assignments, and lab reports. The grading scale is:

A = 90-100% (4) Lecture exams 125 pts ea = 500

B = 80-89% (5) Lecture quizzes 20 pts ea = 80 (1 dropped)
C = 70-79% (1) Written assignment 20 pts = 20
D = 60-69% (2) Lab exams 80 pts ea = 160
F = Below 60% (1) Lab final 100 pts = 100
(5) Lab quizzes 10 pts ea = 40 (1 dropped)
Lab assignments 70 pts total = 70
Lab unknown 30 pts total = _ 30
1000 pts total

Make-up exams: Only one make-up lecture exam will be allowed during the semester. Based on an
excused absence…you must contact me the day of the exam. Make-ups may be essay exams.
Note: You must take all lab practical exams during the time/day it is given, there is no way to
“make-up” a lab practical exam on days they are not given. There are no make-up quizzes in
lecture or lab. If you miss a quiz it will count as dropped quiz.

Academic dishonesty on exams or quizzes will result in a score of zero for that exam or quiz and
could be cause for an instructor drop (See the campus policy regarding academic dishonesty). Do
not give or accept help during an exam!
Bio210 – Spring 2011 (Revised 1/27/2011)
Lecture has a total of 600 points which come from 4 exams, 5 quizzes (lowest quiz score dropped),
and 1 written assignment.

Points for lab total 400 and include 2 midterm exams, a final exam, 5 quizzes (lowest quiz score
dropped), assignments, and an unknown project. Late lab assignments may not be accepted or will
be given a penalty depending on the time frame of the assignment.

Please note that all quizzes (lecture and lab) are given at the beginning of the period and take about
20 minutes. If you are late, you will not be given extra time to complete your quiz. There are no
make-up quizzes.

Note: Orange Coast College policy on attendance states: "...a student who has been absent for 3
consecutive class meetings in a given section is to be dropped by the instructor.”

Students in need of accommodations in the college learning environment:

Tutoring for many different programs including Microbiology and English Language are
available in the Student Success Center located directly across from the microbiology teaching lab
(C&L 108).

Any student who feels he/she may need an accommodation based on the impact of a learning
disability or other disability should contact Disabled Students Programs and Services (DSP&S) in
the Special Services Building to schedule an appointment.

Bio210 – Spring 2011 (Revised 1/27/2011)

 Assigned
Week Date General Microbiology Lecture Topics Chapters

1 T Feb 1 Introduction 1
R Feb 3 History & Nomenclature 1
2 T Feb 8 Chemistry 2
R Feb 10 Chemistry 2
3 T Feb 15 Lecture Quiz 1; Prokaryotes & Eukaryotes 4
R Feb 17 Prokaryotes & Eukaryotes 4
4 T Feb 22 Microbial Metabolism 5
R Feb 24 LECTURE EXAM 1 1, 2, 4
Sa Feb 26 ****Last Day to Withdraw to Avoid a “W” ****
5 T Mar 1 Microbial Metabolism 5
R Mar 3 Microbial Metabolism 5
6 T Mar 8 Lecture Quiz 2; Microbial Growth 6
R Mar 10 Control and Antimicrobial Drugs 7, 20
7 T Mar 15 Control and Antimicrobial Drugs 7, 20
R Mar 17 Lecture Quiz 3; Control and Antimicrobial Drugs 7, 20
8 T Mar 22 Microbial Genetics 8
R Mar 24 LECTURE EXAM 2 5, 6, 7, 20
T Mar 29 Spring Break, Campus Closed
R Mar 31 Spring Break, Campus Closed
9 T Apr 5 Microbial Genetics 8
R Apr 7 Microbial Genetics 8
10 T Apr 12 Eukaryotes: Fungi, Algae, and Protozoa 12
R Apr 14 Eukaryotes: Fungi, Algae, and Protozoa 12
11 T Apr 19 Lecture Quiz 4; Eukaryotes: Fungi, Algae, and Protozoa 12
R Apr 21 Virology 13
12 T Apr 26 Virology 13
R Apr 28 Virology 13
Sa Apr 30 ****Last Day to Drop****
13 T May 3 Microbial Pathogenesis 15
R May 5 LECTURE EXAM 3 8, 12, 13
14 T May 10 Microbial Pathogenesis 15
R May 12 Microbial Pathogenesis 15
15 T May 17 Lecture Quiz 5; Immunology 16, 17
R May 19 Immunology / Vaccines 17, 18
16 T May 24 Immunology / Vaccines 17, 18
R May 26 LECTURE EXAM 4 15, 16, 17, 18

Bio210 – Spring 2011 (Revised 1/27/2011)

 Lab
WK Date Projected Lab Topics
Exercises
1 M/T Jan 31/1 Orientation; Lab safety; Microscopy handout; 3-1
View slides of colored threads, blood smears, and the letter “e”
Set up environmental plates (Incubate at 37°C) 2-1
W/R Feb 2/3 Wet mounts of cheek cells; Aseptic technique, Simple stain 1-3, 3-5
Check and discuss environmental plates (Observe Bacteria) 2-1; 2-2
*Continue growing environmental plates at room temp.

2 M/T Feb 7/8 Isolation of bacteria (Part I): Streak plate #1 1-4
Negative Stain; Gram stain 3-6; 3-7
W/R Feb 9/10 Check Streak Plate #1 and set up Streak Plate #2 1-4
Yeast and filamentous fungi: Tape mounts; Slide cultures (demo ) 12-1; 12-2
Check and discuss environmental plates (Observe - Fungi) 2-1; 2-2

3 M/T Feb 14/15 Quiz 1; Isolation of bacteria (Part II): Standard plate count 6-1
Endospore stain; Check Streak plate #2 3-10
W/R Feb 16/17 OF-glucose; PR-broth; Capsule stain; Acid-fast stain 5-2; 5-3; 3-9; 3-8
Read results for Standard plate count

Carbohydrate Catabolism/Fermentation; Respiration

4 M/T Feb 21/22 Holiday – Washington’s Birthday, No Lab
W/R Feb 23/24 IMViC (Indole/MRVP/Citrate) 5-20, 5-4, 5-8
Starch hydrolysis; ONPG 5-12; 5-18
Read results for OF-glucose, PR-broth; Preparation of media (demo) 1-2

5 M/T Feb 28/1 Quiz 2; Read results for IMViC, Starch hydrolysis, and ONPG
W/R Mar 2/3 Lab Practical Exam #1

Protein Catabolism
6 M/T Mar 7/8 Decarboxylase; Phenylalanine; Urease; Casease 5-10; 5-11; 5-13; 5-14
TSI (H2S only) 5-21 (and 5-20)
W/R Mar 9/10 Gelatinase; Motility/Indole/Ornithine (MIO - similar to SIM) 5-15; 5-20 & handout
Read results for Phenylalanine, Urease, Casease, and TSI (HSS only)

Respiration
7 M/T Mar 14/15 Anaerobic respiration: Anaerobic jar, Nitrate reduction 2-8, 5-7
Aerobic respiration: Catalase, Oxidase 5-5, 5-6
Read results for Decarboxylase, Gelatinase, MIO
W/R Mar 16/17 Set up Temperature exp.; pH demo; Osmotic pressure demo 2-9; 2-10; 2-11
Thioglycollate broth demo 2-7
Read results for the Anaerobic jar and Nitrate reduction
*Remember antiseptics/disinfectants for next class

Control of Microbial Growth

8 M/T Mar 21/22 Quiz 3; UV light; Disinfectants and antiseptics 2-13, 2-14
Antimicrobial susceptibility test (Kirby-Bauer method) 7-3
Read results from the Temperature experiment
W/R Mar 23/24 View Protozoan slides 12-3
Read UV, Disinfectant/antiseptic, and Kirby-Bauer plates
**Remember water samples for next class
Bio210 – Spring 2011 (Revised 1/27/2011)
 Lab
WK Date Projected Lab Topics
Exercises
M/T Mar 28/29 Spring Break, Campus Closed
W/R Mar 30/31 Spring Break, Campus Closed

Microbes in Food/Water & Bacteria of the GI -Tract

9 M/T Apr 4/5 Set up Water Analysis: Membrane filter technique, LTB tubes 8-12; 8-13
Parasite video; View Protozoan slides (continued) 12-3
Unknown project begins – Streak onto TSA & MacConkey’s agar 7-7, 4-5
W/R Apr 6/7 Water Analysis (continued): Set up BGLB tubes 8-13
Epidemiology experiment; Finish protozoan slides 7-6; 12-3
Read results for Membrane filters and LTB tubes
Unknown projects: Look for colony isolation and set up NA slants,
Gram stain each colony type

10 M/T Apr 11/12 Quiz 4; Read results for Epidemiology and BGLB tubes 7-6; 8-13
Review
W/R Apr 13/14 Lab Practical Exam #2

Bacteria of the Skin, Respiratory Tract, and Mouth

11 M/T Apr 18/19 Nasal swab on MSA; Throat swab on blood agar 4-4; 5-25
Set up CAMP tests with Bacitracin test (A-disk), 5-26; 5-24
Optochin (O-disk); Bile Esculin 5-24; 4-3
W/R Apr 20/21 Nasal swab isolate: Coagulase test, Gram stain 5-27; 3-7
Throat swab isolate: Gram stain and identification handout
Read CAMP tests; View hemolysis patterns demo 5-26
Bacteria of the mouth (discussion only)

12 M/T Apr 25/26 Virus video; Set up Plaque Assay 6-5

Read results for nasal/throat isolates, Work on unknown projects
W/R Apr 27/28 Read results for Plaque Assay, Work on unknown projects

Bacteria of the Urogenital Tract

13 M/T May 2/3 Urinalysis: Bactercult and Hurbi biplate 6-2
Triple Sugar Iron (TSI) Agar 5-21
W/R May 4/5 Read results for Bactercult, Hurbi biplate, and TSI
View Helminth slides (if time permits)

14 M/T May 9/10 Quiz 5; Blood smear differentials; Blood typing 11-1; 11-5
View Helminth slides 12-4
W/R May 11/12 Finish Helminth slides 12-4

15 M/T May 16/17 Review; Finish/work on unknown projects

W/R May 18/19 Final Lab Practical Exam (comprehensive)

16 M/T May 23/24 Clean up the lab; Finish all lecture material
W/R May 25/26 No Lab; Lecture Final Exam

Bio210 – Spring 2011 (Revised 1/27/2011)

SAFETY ISSUES IN THE BIOLOGY 210 MICROBIOLOGY LABORATORY

1. Do not come to lab late, new procedures (which often involve safety) are discussed at the beginning of lab.

2. Wear a lab coat during all experimental procedures in the lab. Store your coat on the coat rack or in an
assigned drawer.

3. Be aware of Bunsen burner flames. Turn off burners when not in use. Secure long hair to prevent accidental
exposure to open flames. Report burns and other injuries to your lab instructor immediately.

4. Use the eye wash station immediately, should any reagent accidentally get into your eyes.

5. Do not eat, drink, or store, food/drinks in the lab.

6. Place contaminated items in autoclavable containers for disposal. Do not mix glass with plastic items.

7. Use the biohazard bag to dispose of biological waste…DO NOT use the trash basket to dispose of biological
waste.

8. Remove old cultures from the refrigerator or incubator and place them in the biohazard bag for disposal.
Remove labels from glassware when disposing. Do not remove cultures from the lab or place them in your
lab drawer.

9. Report accidental spills of cultures to your instructor immediately. Do not attempt to pick up broken glass
yourself.

10. Try to avoid bringing your hands to your mouth while in lab.

11. Wash your hands with soap and water before leaving lab.

12 Cover cuts on exposed skin with a sterile bandage.

13. Disinfect your desktop at the beginning and end of lab.

14. Inform your lab instructor if you are pregnant or if you are immunosuppressed as some lab exercises may
pose unique problems in such cases.

15. Wear goggles when using reagents and when near ultraviolet lights.

16. Use proper aseptic technique when making culture transfers.

Bio210 – Spring 2011 (Revised 1/27/2011)