Professional Documents
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7, 599–603
doi:10.1006/cbir.2002.0883, available online at http://www.idealibrary.com on
XINCHEN ZHANG1, HUSHENG ZHANG1*, CONGYI ZHENG2, CHAOYANG LI2, XINSONG ZHANG3
and WEI XIONG4
1
Biomedical Physics Unit, Department of Physics, Wuhan University, Wuhan 430072, China; 2College of Life
Science, Wuhan University, Wuhan 430072, China; 3Business School, Wuhan University, Wuhan 430072 China;
4
Institute of System Engineering, Huazhong University of Science & Technology, Wuhan 430074, China
Extremely low frequency (ELF) pulsed-gradient magnetic field (with the maximum intensity of
0.6–2.0 T, gradient of 10–100 T · M 1, pulse width of 20–200 ms and frequency of 0.16–1.34 Hz
treatment of mice can inhibit murine malignant tumour growth, as seen from analyses at
different hierarchical levels, from organism, organ, to tissue, and down to cell and macro-
molecules. Such magnetic fields induce apoptosis of cancer cells, and arrest neoangiogenesis,
preventing a supply developing to the tumour. The growth of sarcomas might be amenable to
such new method of treatment. 2002 Elsevier Science Ltd. All rights reserved.
K: extremely low frequency (ELF) pulsed-gradient magnetic field; malignant tumour;
inhibitory; biological levels.
Table 1.
Comparison of weights of mice and sarcomas of treated and control groups
Groups Weight of the mice (XSD)/g Weight sarcomas
(SD)/g
0 day 5 day 10 day 15 day 20 day
Fig. 1. Treated sample, No. 2, 1.0 g. (A) Hard sarcoma Fig. 2. Control sample, No. 16, 3.3 g. (A) Softer sarcoma
with the surface covered with viscid membrane. 2.5. (B) joined tightly to its surroundings. 2.5. (B) Apoptotic cells
Detection of apoptotic cancer cell TUNEL. Green shows which make up 4.2% of the total. 200. (C) Left: large
apoptotic cells, which make up 19.3% of the total. 200. nucleus of a cancer cell. Right: well-developed surface
(C) Left: heterochromatin in a nucleus of cancer cell condensed endoplasmic reticulum. 7200.
and coagulated together along the nuclear membrane. Right:
expanded endoplasmic reticulum. 7200.
were more easily cut off from the surrounding
tissues. Their surfaces were covered with a viscid
statistical difference appeared within 20 days capsular membrane (Fig. 1(a)). Sarcomas in
(Table 1). the control group were much larger and softer.
Lacking an intact viscid membrane, they seemed
to be joined tightly with the surrounding tissues
Organs and tissues
(Fig. 2(a)).
Sarcomas from treated mice were smaller and Endothelial cells of the blood vessel were swollen
harder than those from controls (Table 1). They in the treated mice tumours (Fig. 3), which seemed
602 Cell Biology International, Vol. 26, No. 7, 2002
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