HOLLAND HIGH SCHOOL

MID- TERM EXAMINATION

In Preparation of the CSEC Exam
Biology

Paper 2
Time 2 hrs

Instructions: This paper consist two sections, A & B. Section A

has TWO questions and section B TWO questions. You are
expected to answer all FOUR questions.

Question # 1

Catalase is an enzyme that is found in the tissues of living

organisms. This enzyme protects body tissues by breaking down
hydrogen peroxide (H2O2) which is toxic; into water (H2O) and
oxygen gas (O2) so that body tissues are not damaged.

Note: whenever a gas is produce in a reaction, bubbles are

usually seen.

A student carried out an experiment using pieces of rat liver to

investigate the activity of catalase under different conditions.
The student placed the rat liver in hydrogen peroxide under
different conditions.

Observe the students results in the table below and answer the
questions which follow.

Liver in H2O2 + Boiled liver in Crushed liver Whole liver in

hydrochloric H2O2 in H2O2 H2O2
acid
No bubbles Very Small Excessive Moderate
seen amount of amount of amount of
bubbles were bubbles were bubbles were
slowly being rapidly being formed
made made

Figure 1. Results obtained by students after experiment.

Based on the results, give a reason no bubbles were seen for liver
placed in hydrochloric acid.

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By analyzing the results for crushed liver and whole liver, explain
the reason for difference in the results obtained.
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Based on the results for the boiled liver in H2O2, state the
characteristic of enzymes that can be used to explain the reason
for observation gained.

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In the experiment what substance would be considered the

substrate?

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In the experiment what substances would be considered the

products?

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If catalase was a digestive enzyme where in the alimentary canal

would it most likely NOT be found?

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The graph below represent the activity of an enzyme controlled

reaction.

Time taken
for rxn to occur

0 5 10 15 20 25 30 35 40 45
0
50 temp/ C
From the diagram above, account for the shape of the graph.

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Rate of rxn B

0 1 2 3 4 5 6 7 8 9 10
11 12 13 14 pH
From this graph identify the optimum pH value of the enzyme
controlling reaction A, B & C.

A:_______________ B:________________ C:_________________

From this graph where in the alimentary canal would you most
likely find the enzyme controlling reaction C.
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Give the possible name of the enzyme controlling reaction A.

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Based on this graph give the possible name of the enzyme

controlling reaction B ______________________

Give the possible substrate and end product of digestion for

enzyme controlling reaction B.
Substrate: ___________________ product______________________
Respiration is important for living organisms; write the chemical
equation for aerobic respiration.

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Using the table below differentiate between aerobic and

anaerobic respiration.

Aerobic respiration Anaerobic respiration

Give three advantages of ATP

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Examine the table below and answer the questions which follow.

Gases in air % gas in Inhaled air % gas in exhaled air

Oxygen 21 16
Carbon dioxide 0.03 4
Table showing the differences in inhaled and exhaled air for the
gases O2 and CO2
Account for the difference in the percentage oxygen and carbon
dioxide found in inhaled and exhaled air.

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In the space provided use a labeled diagram only to illustrate

the how deoxygenated blood becomes oxygenated.

Label the diagram below

Having a balance diet is necessary for the maintenance of a

healthy body. Therefore, lack of certain nutrients can lead to the
development of certain deficiency diseases. Complete the table
below

Deficiency Disease Symptoms

Vitamin B12 Beri Beri Nervous disorder
Anemia

Vitamin C
Label and annotate the diagrams below