OBJECTIVE:

To obtain a full time position requiring my engineering and scientific knowledge

for the development, characterization, modeling, analysis and improvement of bi
omaterials research.
EDUCATION:
* University of Nebraska Lincoln, Lincoln, NE GPA: 3.80/4.00
Ph.D. Chemical and Biomolecular Engineering (May 2010
)
Thesis: Design and Development of in-situ Albumin Binding Surfaces: Evaluation i
n the Paradigm of Blood-Biomaterial Compatibility.
* Indian Institute of Technology (IIT) Bombay, India Major GPA
: 9/10
M.Tech, Metallurgical Engineering and Materials Science (Au
gust 2004)
Thesis: Liposome Encapsulated Magnetic Nanoparticles for Bioapplication.
* University of Calcutta, Kolkata, India Marks: 83%
B.Tech, Chemical Technology (July 2002)
Thesis: Extraction of Dietary Fiber from Rice Bran Oil Meal.
* University of Calcutta, Kolkata, India Marks: 62%
B.Sc, Chemistry. (June 1999)
RESEARCH AND WORK EXPERIENCE:
Department of Chemical and Biomolecular Engineering, University of Nebraska Linc
oln, NE
Post Doctoral Research Associate January 2010- Presen
t
* Investigated impact of ultrasound stimulations (US) on the growth of fibroblas
ts and chondrocytes seeded in chitosan scaffolds. Evaluated the effect of seedin
g densities and scaffolds dimensions.
* Studied the effects of ultrasound on the morphology and cytoskeleton of cell s
eeded scaffolds by SEM and Confocal Microscopy.
* Developed protocols for the histological analysis of cellular constructs.
* Investigated factors impacting long-term storage of platelets.
Graduate Research Assistant Aug2004-January 2010
* Developed strategies to modify silicon surfaces to create self assembled monol
ayers.
* Designed ligand immobilization methods to create albumin affinity surfaces and
developed protocols for surface modification and their characterization.
* Evaluated protein adsorption patterns on ligand-modified surface with 1D & 2D
Gel analysis, ELISA; Platelet adherence estimated on surfaces with LDH assay and
SEM.
* Investigated and modeled in situ protein adsorption kinetics on ligand modifie
d surfaces with Dynamic Ellipsometry.
* Designed experiments to study impacts of albuminated surfaces on intrinsic coa
gulation cascades.
* Studied protein adsorption patterns under low and high shear condition.
* Excelled in technical writing and handled overall laboratory management, organ
ization and maintenance.
* Honed teamwork skills by collaborating with other Ph.D students and professors
. Mentored 5 undergraduates and 3 graduate students.
Graduate Teaching Assistant Aug 2007 - May 2008
* Computation: Mentored 45 undergraduate students in the principles of Chemical
Engineering computations.
* Transport Operation I: Mentored 30 undergraduate students in problem solving.
* Principles and Applications of Catalysis in Reaction Engineering: Mentored 9
graduate students in problem solving.
Department of Metallurgical Engineering & Materials Science, I.I.T Bombay, Mumba
i, India
Graduate Research Scholar Aug 2002 - July 2004
* Synthesized magnetic nanoparticles using co-precipitation, micro emulsion and
thermal decomposition techniques.
* Tailored liposome coated magnetic nanoparticles for hyperthermia application.
* Characterized nanoparticles with powder X-Ray Diffraction, TEM, Cryo-TEM, FTIR
, Dynamic Light Scattering, and Vibrating Sample Magnetometer.
Department of Chemical Technology, University of Calcutta, India
Undergraduate Research Assistant Aug 2001 - March 2002
* Studied oil extraction techniques from seed by solvent extraction methods.
* Assessed the deoiled-meal in terms of fiber, ash, and mineral content, and eva
luated its feasibility as food product.
Internship May - July 2001
Godrej Industries Limited, Vikhroli, Mumbai, India
* Gained functional overview of the refinery's unit operations and completed a p
roject on temperature control in Fatty Acid Distillation Plants.
TECHNICAL EXPERTISE
* Surface/scaffold preparation and synthesis, Mammalian cell culture (Fibroblast
, Chondrocyte), Cell viability assays, Blood processing, 1D and 2D gel electrop
horesis, Western Blots, ELISA, blood coagulation assays, lyophilization, protein
quantification, ultra-filtration, dialysis/concentration, buffer preparation.
* Analytical technique: Confocal/optical microscopy, SEM, AFM, FTIR, Ellipsometr
y, Contact angle measurement, UV-Visible spectroscopy, Densitometer, powder XRD,
dynamic light scattering.
* Computer: MATLAB(R) 7.4, Origin, ChemDraw, Delta2D, GIMP, Excel, Word.

PUBLICATIONS:
* J. Giri, S. Guha Thakurta, J. Bellare, A. K. Nigam, D. Bahadur, "Preparation a
nd characterization of phospholipid stabilized uniform sized magnetite nanoparti
cles", Journal of Magnetism and Magnetic Materials, 293(2005) 62-68.
* S. Guha Thakurta, A. Subramanian, "Effect of Hydrofluoric Acid in Oxidizing Ac
id Mixtures on the Hydroxylation of Silicon Surface", Journal of The Electrochem
ical Society, 154 (2007) 136-146.
* S. Guha Thakurta, R. Miller, A. Subramanian, "Investigation of platelet respon
ses and clotting characteristics of in situ albumin binding surfaces", accepted
in Journal of Biomaterials Applications.
* S. Guha Thakurta, A. Subramanian, "Evaluation of in situ Albumin Binding Surfa
ces: a Study in Protein Adsorption and Platelet Adhesion", accepted in Materials
in Medicine.
* G. I. Hasanova, S.E. Noriega, T. M. Mamedov, S. Guha Thakurta, J. A. Turner an
d A. Subramanian, "The effect of ultrasound stimulation on the gene and protein
expression of chondrocytes seeded in chitosan scaffolds", under review in Tissue
Engineering
* S. Guha Thakurta, A. Subramanian, "Fabrication of dense, uniform aminosilane
monolayer for potential application of protein or ligand immobilization platform
", under review in Journal of Colloidal and Interface Science.
* S. Guha Thakurta, H. J. Viljoen, A. Subramanian, "Protein adsorption kinetics
on peptide modified surface", under review in Langmuir.
* S. Guha Thakurta, R. Miller, A. Subramanian, "Protein adsorption on peptide mo
dified surface under low and high shear condition", manuscript under preparation
.
PRESENTATIONS
* "Bioeffects of Ultrasound Stimulation", Food, Pharmaceutical & Bioengineering,
AICHE 2010 Annual meeting, Salt Lake City, Utah, November 7-12, 2010, to be pre
sented.
* "Analysis of platelet adhesion to in situ albumin binding surfaces under defin
ed shear conditions", Materials Engineering and Sciences, AICHE 2010 Annual meet
ing, Salt Lake City, Utah, November 7-12, 2010, to be presented.
* "Synthesis and Characterization of Albumin Binding Surfaces for Implantable S
urfaces", Society of Biomaterials, San Antonio, Tx. April 22-25, 2009 - Poster p
resentation.
* "Control on platelet adhesion and activation events by selective and specific
binding of plasma protein on peptide coupled surface", Designer Biointerfaces, M
aterials Research Society, St. Louis, MO. March 26, 2008.
ACADEMIC HONORS
* All India 7th rank in Graduate Aptitude Test in Engineering 2002 in Engineerin
g Science with 99.13 percentile.
* Gold medalist, B.Tech 2002, University of Calcutta, India.
* National Talent Search Scholarship from NCERT, Government of India.
LEADERSHIP EXPERIENCE
* Representative of Graduate students of Chemical Engineering Department at GSA-
UNL. 2006-2008
* Finance Secretary, Hostel 11, IIT Bombay. 2003-2004
* Coordinator, Performing Arts Festival - 2003, IIT Bombay. 2003