Mr.

Chung-Wein Lee (Chung)

PROFILE/OBJECTIVE:
A multi-disciplinary and a fast learner with strong analytical ability associa
te. Position with research organization, where my computational Bioinformatics
skills and wet lab research experience in biotech industry can be used to make a
n impact. Record of solving problems independently and in a team. Strong documen
tation and communication skills.
EDUCATION:
M.S. Biophysics (Indiana University, Bloomington IN)
08/97 08/00
M.S. Mathematical Physics, (National Center University, Taipei, Taiwan)
08/95 08/97
B.S. Physics (Soochow University, Taipei, Taiwan 08/90 08/94
BIOINFORMATICS:
Functional Genomics: created yeast cell cycling mechanism modeling; developed p
air-wise gene expression to classify cancer phenotype classification; annotated
and searched gene sequence from DrugMatrix and other Lilly internal databases.
Proteomics: Utilized genetic algorithm and other statistical methods to identif
y cancer protein biomarkers from raw SELDI-TOF-MS spectrum. Simulated time-of-f
light in MS to correct the spectrum shift.
Modeling and imaging analysis: investigated and characterized self-organized sy
stems with various algorithms. Developed imaging markers (such as tortuosity, ve
ssel lumen) to quantify angiogenesis.
COMPUTER SKILLS:
Language C, SQL, PERL, Regular Expression, UNIX Shell language, R, MATLAB
Database Access, Oracle (Drug Matrix)
Software SAS, JMP, Spotfire, Pathway Analysis, Analyze (AnalyzeDirect Inc.),
Image J,
OS Platform MS-XP, Linux Red Hat, Mac
BIOCHEMISTRY/MOLECUALR BIOLOGY:
Extracted, purified actin filament proteins from rabbit muscles. Run SDS gel to
exam the quality of actin proteins. Measured cell mobility force from actin fil
ament polymerization is osmometer. Discovered new actin packing phase state unde
r extra high centrifugation.
Extracted, purified flippase and measured flippase activity. Utilized NMR to qu
antify cholesterol orientation in lipid bilayer. Synthesized lipid bilayers on g
lass and quantified the lipid bilayer surface by Atomic Force Microscope.
Developed short peptide to receptor binding assay with spectrofluorometer and f
lowcytometer. Designed the linker for multimeric peptides.
Synthesized Human/Bovine serum albumin microbubbles and extruded nanoliposomal
CT contrast particles. Conjugated antibody to microbubbles via biotin-avidin tec
hnology.
Plated various cell culturing. Transfected siRNA/plasmid to cells and to mice v
ia sonoporation.

EMPLOYMENT CHRONOLOGY:
RESEARCH ASSOCIATE II, COVANCE INC., GREENFIELD IN 10/08 - 04/10

Project: (1) Searched and reviewed scientific literature on new development on i

maging and bioinformatics applications in preclinical drug discovery.
(2) Streamlined the CT image reconstruction and quality control pr
ocess that customers received results in two days after scheduled CT scan.
(3) Communicated to interdepartment scientists to exam the progres
s the service contract and to exam the quality of results before drafting the fi
nal reports in 24 hours.

COMPUTATIONAL BIOLOGIST, INTEGRATIVE BIOLOGY, LILLY RESEARCH LABORATORIES, ELI L

ILLY AND COMPANY, GREENFIELD IN 10/04 - 10/08

Project: (1) Mined clinical cancer microarray gene expression and pathological d
ata from both external and Lilly internal databases. Developed and programmed pa
ir-wise gene algorithm to classify various phenotypes of DLBCL, lung cancers.
(2) Applied statistics methods and pathway analysis to identify g
enes associated with liver toxicity from Drug Matrix database. Searched gene seq
uence. Annotated genes and proteins in internal drug discovery database.
(3) Developed various 3D *CT imaging markers to quantify normaliz
ation of entire tumor vasculature and evaluate anti-VEGF treatment.
(4) Synthesized both albumin and lipid microbubble and conjugated
antibody to lipid microbubbles to detect related antigen expression in xenograp
h model.
(5) Investigated the optimized ultrasound settings for siRNA and
plasmid sonoporation both in vitro and in vivo.
Achievement:
In Bioinformatics, I searched the databases and programmed all of necessary code
s and applied different software to various Bioinformatics tasks in Lilly. We w
ere invited to Seoul of South Korea to present our novel gene-pair algorithm to
classify phenotypes of cancers in VLDB meeting. We published our Bioinformatics
work in two different journals and were invited to publish in a book chapter th
is year. In March of this year, the book chapter was accepted for publication.
I was awarded for Team Work in Bioinformatics lipoprotein projects in 2005.
In support to Imaging group in Lilly, we successfully developed ultrasound micro
bubble synthesis and researched microbubble applications in both non-invasive im
aging and drug delivery. We not only gained the experiences of sonoporation but
reduced the cost at least 50% compared to contract out to academic labs. We de
veloped our own imaging analysis algorithm to quantify the antigen/receptor expr
essions after acquiring targeted microbubble images. The work was awarded by Lil
ly LRL both in team work and scientific achievement.

BIOINFORMATICS/BIOMECHANICS
DEPARTMENT OF COMPUTER SCIENCE, PHYSICS, CHEMISTRY, DEPARTMENT OF CELL AND ANATO
MY OF IUSM, DEPARTMENT OF BIOLOGY OF IU BLOOMINGTON 08/00 - 06/04

Project: (1) Functional Genomics Yeast Cell Cycling cDNA Microarray Analysis An
d Modeling (Project of Computer Science and BME)
(2) Proteomics Ovarian Cancer Prognostic Biomarker Search And Pa
rathyroid Hormone Treatment Evaluation By Analyzing SELDI-TOF-MS data (Ciphergen
ProteinChip Technology) (Project of Computer Science and Cancer Center)
(3) NMR/AFM/NSOM investigation on lipid orientation and perfusio
n on artificial liposome (Project of Physics and Biology Department)
Achievement: We developed proteomics data analysis algorithm to search proteins
from SELDI-TOF-MS spectrum with methods in Physics and Statistics. We used thi
s algorithm to analyze the SELDI proteomics data for Cancer and Bone group. I t
ransformed the yeast gene expression values to phase space to characterize the m
echanism of yeast cell cycling. Six papers and conference reports at different
topics were published.

RECOMMENDATION LETTER CONTACT:

(1) Dr. Shuyu Dan Li, Principle Research Scientist, Group Leader, Informatics, T
ranslational Science, Lilly Corporate Center, Indianapolis IN, Li_Shuyu_Dan@lill
y.com, 317-651-9923 (phone preferred)
(2) Dr. Birong Liao, Director Data Science/Solutions Clinical Development Organ
ization, Lilly Corporate Center, Indianapolis IN, Liao_Birong@lilly.com, 317-655
-1717 (phone preferred)
(3) Dr. John Calley, Principle Research Scientist, Genome sequencing, Lilly Corp
orate Center, Indianapolis IN, john@calleys.org , 317-433-3399
(4) Dr. Eric W. Su, Principle Research Scientist, Lilly Corporate Center, Indian
apolis IN, ewsu@lilly.com, 317-277-7706

ESSENTIAL GRADUATE COURSES:

Cell Biology, Biochemistry, Molecular Biology, Biophysics, Special topics in Ch
emsitry, Human Physiology and Anatomy, Statistical Methods, Diagnostic Medical I
maging, Automata and Formal Language.

PUBLICATIONS AND MEETING PRESENTATIONS:

One thesis; one book chapter; five meeting conference reports; 11 journal publi
cations (8 of them are as first/last author). Detail list will be sent upon req
uest.