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Species No. of clinical IDE 32C AUX RAPD/PCR Germ tube Clamidospores
isolates tested
C. albicans 17 17 20 15 13
C. dubliniensis 2 2 0 0 2
C. glabrata 14 14 14 0 0
C. guilliermondii 10 10 10 0 0
C. kefyr 8 8 7 0 0
C. krusei 6 6 7 0 0
C. lusitaniae 9 9 11 0 0
C. parapsilosis 8 8 5 0 0
C. tropicalis 15 15 15 7 2
C. rugosa 1 1 2 0 0
C. pelicullosa 1 1 1 0 0
C. colicullosa 1 1 0 0 0
TOTAL 92 92 92 22 17
Table 3. Sensitivity and specificity of RAPD/PCR for the identification of species of Candida and the biological test for
identification of Candida albicans
Species No. RAPD/PCR Primer OPE-18 Germ tube Clamidospores
S E VP + VP - S E VP + VP - S E VP + VP -
C. albicans 17 100 0.96 0.84 0.99 0.88 0.91 0.67 0.97 0.76 0.97 0.86 0.95
C. dubliniensis 2 0 0 NA NA
C. glabrata 14 100 100 NA NA
C. guillermondii 10 100 100 NA NA
C. kefyr 8 0.87 100 100 0.99
C. krusei 6 100 0.98 0.99 100
C. lusitaniae 9 100 0.97 0.99 100
C. parapsilosis 8 0.62 100 100 0.96
C. tropicalis 15 100 100 NA NA
C. rugosa 1 100 50 NA NA
C. pelicullosa 1 100 100 NA NA
C. colicullosa 1 0 0 NA NA
S=Sensitivity P≥ 0.05 E=Specificity P≥ 0.05
PV += Predictive values positive P≥ 0.05 PV -= Predictive values negative P≥ 0.05 NA=No aplicable
Figure 1. RAPD-PCR performed with genomic DNA (primer OPE-18) from culture collection strains of Candida. The different
independent assays revealed reproducibility of the band patterns
Figure 2. The overall clinical isolates were identified according to species usin genomic DNA (primer OPE-18). RAPD/PCR
patterns of different Candida species were very consistent. The figure shows amplicons from each of individual clinical isolates.
Lanes 51, 60, 62 and 64 were eliminated. The first lane in each serial corresponding to DNA ladder.