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Test 2
Question 2
Which statement is true regarding the virus shown above: A) B) C) D) E) This virus would most likely be a bacteriophage. The capsid of this virus is indicated by "B" Infectivity of this virus is determined by recognition of the host by structures labeled "A". "D" could only be DNA. All the above are true statements.
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B) C) D)
Question 7
br/> A bacterial cell has the metabolic pathway shown above. The genes coding for enzymes 1 and 2 in this pathway are organized into an operon. Enzyme 1 gene and enzyme 2 gene are not transcribed when molecules of "C" are present; for this reason, the operon would be characterized as: A) B) C) D) a repressible operon. a constitutive operon. an inducible operon. lysogenic operon.
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Transcription of a gene can occur at basal (low) levels without the contribution of enhancer sequences or B) proximal control elements. The very first step in the expression of a gene on a chromosome starts with RNA polymerase binding to a C) promoter. D) Any mRNA transcript can be translated as long as it has a 5' cap and 3' polyA tail.
Question 13
The following statements are false concerning exons of eukaryotic genes, except: A) B) C) D) Translation of exons in pre-mRNA occurs in the cytoplasm. All exons are removed during the splicing process. The mature mRNA's of a gene can contain different orders of exons. Introns, not exons, contain translatable coding sequences.
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E)
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A) B)
True False
Question 21
Chromatin remodeling had not occurred in the region of the chromosome containing this gene for this C) protein. D) E) Introns were excised from the transcript of the gene for this protein. Ribosomes aided in translation of the mature mRNA's of the gene for this protein.
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E)
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br/> Using the vector above, you carry out the steps to clone a gene (i.e., restriction enzyme digest with EcoR1, form recombinant DNA, transformation of host cells, growth and selection of host cells) from a species of fish. As you can see there are two antibiotic resistance genes, kanamycin (Kmr) and tetracycline (Tetr) in this vector; the cloning site of the vector is in the kanamycin resistance gene. You finally have your transformed cells and plate them out on antibiotic-containing growth media. What do you know about the cells that are capable of growing on both kanamycin and tetracycline? A) B) C) They contain vector lacking fish DNA segments. They do not contain vector or vector + fish DNA. They contain re-circularized fish DNA segments.
D)
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to nicotine (a carcinogen found in cigarettes), AND he/she wanted to identify those specific genes, then the scientist would: A) B) C) D) E) prepare a cDNA library of liver cells that were exposed to nicotine employ a DNA chip, or microarray, prepared from the rat genome do a PCR reaction of the entire rat genome perform choice A (cDNA library) first, then use this in conjunction with choice B (DNA chip). have to do all three steps (A, B, & C).
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br/> Shown above is an electrophoresis gel performed on two different DNA samples, independently cut with the same restriction enzyme. Sample 1 yielded 3 fragments, A, B, & C; sample 2 produced fragments D, E, F, & G. Which of the following statements is false concerning this gel? A) B) C) D) E) Fragment "C" is the smallest one of both samples. Fragments "A" and "F" are of approximately the same size. Fragment "D" is the largest one of both samples. The movement and migration pattern of these DNA fragments is due to both their charge and size. None of A-D is false they are all true.
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C) D) E)
May involve removal of cells from the patient and inserting the desired gene in these cells. Could involve simply injecting the vector containing the desired gene directly into the patient. Can employ viruses as the vehicle to insert genes into target tissues.
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Involves the transplantation of the nucleus of a humans' somatic cell into an enucleated (nucleus removed) B) donor egg. C) D) E) During the procedure one forms and cultures stem cells of an individual. The goal is to form specialized tissues for medical applications. A-D are all true statements.
Question 38
Which of the following is not true concerning cancer? A) B) Most cancers result as a consequence of the accumulation of mutations in somatic cells. Only some, not all, cancers are known to be caused by viruses.
An increased incidence of cancer could result in an individual if he/she has inherited a mutated allele of a C) tumor suppressor gene. D) E) All forms of cancer have been proven to be heritable. Metastized tumors can spread to other tissues.
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A) B)
True False
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