Code No: RR322302 Set No.

1
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. In brief compare the different types of light microscopes. [16]

2. Write in detail the description of a typical microbiological light microscope. [16]

3. (a) Distinguish between Nucleotides and Nucleosides

(b) Deoxyribose and ribose [8+8]

4. What are the factors affecting electrophoretic mobility of any molecule? [16]

5. Explain the principle in Southern Hybridization. [16]

6. Explain antisera production in detail. [16]

7. Which is the most commonly used Radio Isotope for radio labeling antibodies?
Describe it. [16]

8. What is the difference between BOD and COD? [16]

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Code No: RR322302 Set No. 2
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain the following terms associated with Fluorescent Microscope.

(a) Barrier filter

(b) Heat filter
(c) Fluorochromes
(d) Exciter filter. [4+4+4+4]

2. List out the prerequisites for viewing any biological specimen under TEM. [16]

3. What are Nucleotides? At what wavelength can they be absorbed and why? [16]

4. What is the role of SDS in SDS − PAGE? [16]

5. Write short notes on

(a) Restriction Enzymes

(b) Nitrocellulose filters. [8+8]

6. How is PCR used to sequence DNA? [16]

7. What is meant by Immunoblotting? What is it used for? [16]

8. How is gaseous Oxygen concentrations measured? [16]

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Code No: RR322302 Set No. 3
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe a Dark Field Microscope. [16]

2. Describe the construction of a TEM. [16]

3. How are proteins detected biochemically in any sample? [16]

4. What are the differences between horizontal and vertical Electrophoresis units?[16]

5. Discuss the importance of Elution time; volume and Capacity factor in Chromatog-
raphy. [16]

6. Explain Maxam and Gilbert method of DNA sequencing. [16]

7. Differentiate between single and double Electroimmunodiffusion. [16]

8. What is meant by the term “Biomass”? Explain its relevance to cell growth. [16]

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Code No: RR322302 Set No. 4
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What is Bright Field Microscopy? [16]

2. List out the prerequisites for viewing any biological specimen under TEM. [16]

3. How are proteins detected biochemically in any sample? [16]

4. How is Isoelectric focusing done in protein samples? [16]

5. Explain, “Western blotting is an extension of protein Electrophoresis”. [16]

6. List out the different antigen-antibody reactions, and explain Agglutination in de-
tail. [16]

7. Write short notes on

(a) Rocket Immunoelectroforesis

(b) Radial Immunoassays. [8+8]

8. Explain the terms

(a) Dissolved Oxygen (DO)

(b) Dissolved Carbon dioxide (DCO2 )
(c) Oxygen transfer rate (OTR)
(d) Oxygen uptake rate (OUR). [4+4+4+4]

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