10% bleach solution biohazard materials (blood, non heat fixed bacterial smears)

Biohazard "labeled trash container" disposable plastic /or paper disposed a-b/b*100 all organisms considered pathogenic

autoclave Air sampling - SABS antiseptic aseptic

broken glass box - glass slides in the plastic container with bleach degerming disinfectant fastidous organisms requires supplement enrichment fixation glogerm - non toxic product that stiumlates bacteria. Contains ingredients formulated to the same size of the bacteria. Glow under UV light hand washing components, soap/product, time, method, temperature, flow, dry incubator RODAC - replication organisms detection and counting - plates are designed to permit direct contact of agar with flat surfaces of areas to be sampled SAB - sabouraud dextrose agar - culture media for yeats and mold. Has large amount of dextrose sugar and low pH 5.6. Its a selective growth media sanitization screw top glass - gray metal baskets for auto claving + reuse

sterilzation

Surface sampling - TSA using RODAC plates various disinfectants TSA - tryptic soy agar use for isolate non-fastidious organism

Bash staff grass Addis ababa fastidious organisms requres supplement enrichment

air sampling - SABs biohazard SAB

TSA a-b/b*100 hand washing components sanitization

fixation glogerm RODAC air sampling - SABs screw top glass sterilization all organizims considered pathogenic degerming disinfectant incubator sterilization antispetic

biohazard materials

aseptic broken glass box

autoclave

bactiCinerator binocular scope not focus. Make sure ocular is focus to your eyes. Check adjustment between the ocular sets distance between your eyes

black half-moon or quarter-moon. Objective completely clicked can't focus the level of specimen. Make sure slide is face right side up Clean lenses but not clear check condenser if any oil

field is black, diaphragm open. Make sure plugged & cord not block light source fuzzy images in high power. Make sure no oil and clean image too dark. Diaphragm or condeser is up innoculating loops

objective lost when move from low power to high. Make sure the object is in center Air Sampling SABS Surface sampling - TSA - RODAC plates on various disinfectants Water proof marking pens - all labelings should be done incubation of plates - upside down to preven condsenation drip to agar blood smears

BBC Ofi if BBC

bactiCinerator binocular scope black half-moon

objective lost field is black image too dark

innoculation loops fuzzy images

ocular lens stage adjustment knob

Discs of Co. fine adjustment knob

fine adjustment know objective lenses diaphragm level

objective lenses diaphragm level stage

coarse adjustment knowb stage condenser illuminator illuminator condenser stage adjustment knowb

coarse adjustment knowb ocular lens

candida sp bacillus sp

Staphyloccoocus aures Escherichia coli pure culture

Aseptic technique sub culturing - when specimens are transferred between media bacterial smears - a thin layer of bacteria on a slide borth culture

solid meida oil immersion control slide heat fix candida sp streptoccocus ferment lactose oxidase test enzyme cytochrome oxidase enrichment media - adds various components to the basic nutrient for fastidious organism selective media utilizez ingreadient that will ihibit some and grow some organisms differential media - allows visual of physical diffence of organism that grow

macconkey agar (MAC) - is a slective and differential media. Has salts, neutral red dye and lactose. Inhibits gram positive orgnism. (ferment of lactose or not) LF - reduce in pH, hints acidic, organism that absorb will show more red , lactose fermented. Drop in pH leads to bile salts NLF - colorless and translucent on MAC HEA - Hektoen Enteric Agar - is selective and differential media. To tell the difference of Salmonella and Shigella. NLF - green to blue color. NLF produce Hydrogen Sulfide with a black center positive oxidase positive - presence of enzyme cytochrome oxidase salmonella sp. enterobacter sp. proteus sp pseudomonas aeruginosa Aseptic technique streak for isolation subculture citrate tube if oxidase is positive typically it's GF Drop the oxidase reagent on filter paper then rub the organism to be tested into the reagent with applicator stick. Or can use cotton tipped applicator and add the reagent directly to the bacterial swab. MUST Observe the color change 10-30 seconds Kligler's Iron Agar - determined if ferment lactose or ferment glucose or ability to produce hydrogen sulfide citrate - biochemical test used to determine organism can use their sole carbon resource/ the only source for carbon. Indicator from green to blue is POSITIVE PDA - must add the 10% Ferric Chloride. Phenylalanine agar. Positive means presence of enzyme. Tan color to Green is POSITIVE KIA - red slant on tube means an indication of alkaline reaction, and yellow butt is GF Citrate - no color change equal negative ie GREEN, if color change to blue it's positive on the slant PDA - no color change of the slant = negative, if color is changed to green then positive

Kirby Bauer method - qualitative method used medium called Mueller Hinton, pH of medium, depth medium, size of the inoculum and the amount of antibiotic used. Zone size may vary. .5 mcFarland standard ~ turbidity ~ broth Sensitivity is same as susceptibility ~ means there is zone inhibition KB is for both positive and negative bacteria API 20E - gram negative differentaite between staph aures and staph sp. Is by using latex agglutination and differntial media mannitol salt agar (MSA) catalase vs oxidation streptococci - hemolysiss patterns and susceptibility. Catalase test - gram positive Latex agglutination test = clumping equals postive for staphylococcus aures. No clump is negative so not aures MSA - mannitol salt agar - if yellow it's Staphyloccos aures.