Professional Documents
Culture Documents
Therapeutic Applications
of Whey Protein
Keri Marshall, ND, MS
Whey Protein Concentrate Ranges from 25-89% Some fat, lactose, and minerals
Most commonly available as 80% As protein concentration increases, fat,
lactose, and mineral content decreases.
available commercially. Each whey product var- and incubated for 30 minutes. Rennet extract is
ies in the amount of protein, carbohydrates, im- added and the mixture is stirred, resulting in co-
munoglobulins, lactose, minerals, and fat in the agulation of curd.
finished product. These variables are important Rennet is derived from the abomasum
factors in the selection of whey fractions for spe- (fourth stomach) of newly born calves. Chymosin,
cific nutritional applications. Table 1 describes the the active enzyme ingredient of rennet, aids in the
various whey protein products available. coagulation of milk by separating it into curds and
whey. In a newly born calf, chymosin aides in the
Whey Protein Manufacturing digestion and absorption of milk. Adult cows do
not have this enzyme.
Protein from bovine whole milk consists
The liquid whey is drained through a stain-
of approximately 20-percent whey protein. When
less steel screen and the remaining curd is cut and
casein is removed from whole milk, liquid whey
cooked at 30 degrees C. Whey liquid is then fil-
remains, having a protein concentration of about
tered at 45 degrees C and brought to a pH of 3 by
65 percent. The following is a summary of the
adding citric acid. The liquid is filtered to one-
Ohio State University method of manufacturing
fifth its original volume, resulting in whey con-
whey protein powder. Milk is high-temperature,
centrate that is approximately 80-percent protein.
short-time pasteurized (163 degrees F for 30 sec-
This can be additionally micro-filtered to increase
onds) and held overnight at 40 degrees C. The
protein concentration to as high as 95 percent.
following morning the mixture is cooled to 30
degrees C, inoculated with a lactic acid culture,
Table 2. Amino Acid Residues in Bovine versus Human fat and lactose. In addition,
some manufacturers hydro-
Lactoferrin lyze (cleaving peptide bonds
via enzymes or heat) the whey
Bovine Lactoferrin Human Lactoferrin to provide more peptides and
free amino acids in the final
Alanine 67 63
product.4
Proline 30 35 The commercial suc-
cess of whey protein has led
Arginine 39 43
to the development of high
Lysine 54 46 quality whey protein supple-
Asparagine 29 33 ments manufactured as pri-
mary products and not as a by-
Valine 47 48 product of cheese manufactur-
Tryptophan 13 10 ing. Manufacturers take spe-
cial care to preserve the bio-
Cysteine 34 32 logical activity, native protein
Threonine 36 31 structure, and protein-bound-
fats in the finished product.
Isoleucine 15 16 Proteins are processed under
Serine 45 50 low temperatures and not ex-
posed to fluctuating pH
Glutamine 29 27 changes to avoid denaturing
Glutamic acid 40 42 the native structures. In addi-
tion, the source of milk and the
Phenylalanine 27 30 health of the milking cows is
Methionine 4 5 thought to contribute to im-
mune-enhancing activity of
Leucine 65 58 whey products.5,6
Glycine 48 54
Tyrosine 22 21
Biological
Components
Aspartic Acid 36 38
Amino Acid Content
Histidine 9 9 Collectively, whey
Total number of residues 689 691 proteins have all the essential
amino acids and in higher con-
From: Pierce A, Colavizza D, Benaissa M, et al. Molecular cloning and centrations compared to vari-
sequence analysis of bovine lactoferrin. Eur J Biochem 1991;196:177-184. ous vegetable protein sources
such as soy, corn, and wheat
gluten.2 In addition to having
a full spectrum of amino ac-
The final whey protein concentrate is ids, the amino acids found in whey are efficiently
warmed and spray-dried to achieve whey protein absorbed and utilized, relative to free amino acid
powder. Whey protein concentrates can then be solutions.7
put through an ion-exchange process to remove
Relative to other protein sources, whey breast-feeding, and serves as a child’s first line of
has a high concentration of branched-chain amino immune defense – referred to as “passive
acids (BCAAs) – leucine, isoleucine, and valine. immunity.” IgA is secreted in breast milk and
BCAAs, particularly leucine, are important fac- ultimately transferred to the digestive tract in the
tors in tissue growth and repair. Leucine has been newborn infant, providing better immunity than a
identified as a key amino acid in protein metabo- bottle-fed child.12 Colostrums contain significantly
lism during the translation-initiation pathway of greater concentrations of immunoglobulins than
protein synthesis.8 mature milk. Immunoglobulins reach maximum
Whey proteins are also rich in the sulfur- concentration the first 24-48 hours post-parturition
containing amino acids cysteine and methionine. and decline in a time-dependent manner following
With a high concentration of these amino acids, peak concentration.13
immune function is enhanced through intracellu- Similarly, the whey fraction of milk ap-
lar conversion to glutathione. pears to contain a significant amount of immuno-
globulins, approximately 10-15 percent of total
Lactoferrin whey proteins. An in vitro study demonstrated bo-
Lactoferrin, an iron-binding glycoprotein, vine milk-derived IgG suppresses human lympho-
is a non-enzymatic antioxidant found in the whey cyte proliferative response to T cells at levels as
fraction of milk as well as in colostrums. The low as 0.3 mg/mL of IgG. The authors further con-
lactoferrin component of whey consists of approxi- clude bovine milk IgG typically ranges between
mately 689 amino acid residues, while human 0.6-0.9 mg/mL and is therefore likely to confer
lactoferrin consists of 691 residues. 9 Whey immunity that could be carried to humans.14 Stud-
lactoferrin is composed of a single polypeptide ies show raw milk from non-immunized cows con-
chain with two binding sites for ferric ions. Be- tain specific antibodies to human rotavirus, as well
fore processing, bovine lactoferrin is only 15-20 as antibodies to bacteria such as E. coli, Salmo-
percent saturated with iron. Iron-depleted nella enteriditis, S. typhimurium, and Shigella
lactoferrin, defined as containing less than five- flexneri.15,16
percent iron, is referred to as apolactoferrin. Hu-
man breast milk contains apolactoferrin.10 The beta-Lactoglobulin
concentration of lactoferrin in human milk and beta-Lactoglobulin represents approxi-
colostrums is approximately 2 mg/mL and 7 mg/ mately half of the total protein in bovine whey,
mL, respectively, while in bovine milk and colos- while human milk contains no beta-lactoglobu-
trums it is approximately 0.2 mg/mL and 1.5 mg/ lin. Besides being a source of essential and
mL, respectively.11 Lactoferrin is a dominant com- branched chain amino acids, a retinol-binding pro-
ponent of whey protein in human breast milk; tein has been identified within the beta-lactoglo-
however, the concentration in most commercial bulin structure. This protein, a carrier of small
whey protein powders is only 0.35-2.0 percent of hydrophobic molecules including retinoic acid, has
total proteins. Table 2 illustrates the difference in the potential to modulate lymphatic responses.17
amino acid profiles between bovine and human
lactoferrin. alpha-Lactalbumin
alpha-Lactalbumin is one of the main
Immunoglobulins proteins found in human and bovine milk. It
An immunoglobulin (Ig) is an antibody comprises approximately 20-25 percent of whey
or gamma-globulin. There are five classes of proteins and contains a wide variety of amino
antibodies – IgA, IgD, IgE, IgG, and IgM. IgG acids, including a readily available supply of
constitutes approximately 75 percent of the essential and branched chain amino acids. Purified
antibodies in an adult. IgG is transferred from alpha-lactalbumin is most readily used in infant
mother to child in utero via cord blood and by
Bovine Serum
Albumin Figure 1. Synthesis of Glutathione from Cysteine, Glutamate,
Bovine serum and Glycine
albumin (BSA) is a
large protein that makes
up approximately 10-15 Step 1 Glutamic acid Step 2 g-Glutamylcysteine
percent of total whey + +
protein. BSA is a source Cysteine Glycine
of essential amino + +
acids, but there is very ATP ATP
little available informa-
g-Glutamylcysteine Glutathione
tion regarding its poten-
synthetase synthetase
tial therapeutic activity.
Table 3 sum-
marizes the compo-
nents found in whey. g-Glutamylcysteine Glutathione
+ +
ADP ADP
Mechanism of + +
Action P P
Whey has po-
tent antioxidant activ-
ity, likely by contribut-
ing cysteine-rich pro-
teins that aid in the synthesis of glutathione (GSH), initiation. Practitioners use whey protein products
as a source of cysteine to increase intracellular
a potent intracellular antioxidant.2 GSH is com-
glutathione levels25,26 and it has been reported that
prised of glycine, glutamate, and cysteine (Figure
1). Cysteine contains a thiol (sulfhydryl) group GSHPx activity in cow’s milk, and presumably
whey, is the same as in human milk.27
that serves as an active reducing agent in prevent-
ing oxidation and tissue damage. As an antioxi-
dant, glutathione is most effective in its reduced
Studies on lactoferrin have demonstrated increasing hydrolysis and slowing entrance to the
its ability to activate natural killer (NK) cells and small intestine. Whey proteins, on the other hand,
neutrophils, induce colony-stimulating factor ac- do not coagulate under acidic conditions. They are
tivity, and enhance macrophage cytotoxicity.28-31 considered to be “fast proteins,” as they reach the
Lactoferrin also appears to have antivi- jejunum quickly after entering the gastrointesti-
ral, antifungal, and antibacterial properties. The nal tract.40 After reaching the small intestine, the
antimicrobial effect is likely more potent in or- hydrolysis of whey is slower than that of casein,
ganisms that require iron to replicate, as lactoferrin allowing for greater absorption over the length of
has the unique ability to chelate iron in a way that the small intestine.
deprives microorganisms of this essential nutri- A study by Arnal et al demonstrates that
ent for growth.32 In additional, lactoferrin has the whey’s rapid absorption patterns are superior for
ability to release the outer membrane of gram- postprandial protein utilization and overall nitro-
negative bacteria, the lipopolysaccharide compo- gen balance in elderly women.41 Most recently, a
nent, thus acting as an antibiotic.33 randomized, single-blind study examining protein
Lactoferrin demonstrates anti-inflamma- satiety found whey protein to exhibit a higher post-
tory properties. A mouse study revealed lactoferrin prandial level of plasma amino acids compared to
had the ability to regulate levels of tumor necro- casein.42
sis factor (TNF) and interleukin 6 (IL-6), thus A study by Troost et al examined the ef-
decreasing inflammation and, ultimately, mortal- fects of an orally administered recombinant hu-
ity.34 man lactoferrin (rhLF) in the digestive tract. The
In addition to the above-mentioned prop- study population consisted of eight female ileo-
erties, alpha-lactalbumin can chelate heavy met- stomy patients who consumed 5 g rhLF and col-
als.35 It reduces oxidative stress because of its iron- lected full ileostomy output for 24 hours. In a 24-
chelating properties.36 hour period, only 4 µg of lactoferrin were excreted.
Whey has been recently touted as a health- The study concluded dietary rhLF does not reach
ful dietary supplement to reduce blood pressure. the colon because it is digested in the stomach and
Antihypertensive peptides have been isolated in small intestine.43 A similar study by the same group
the primary sequence of bovine beta-lactoglobu- was conducted on 12 healthy volunteers. On three
lin.37 These peptides give whey significant angio- separate days, volunteers were instructed to con-
tensin I converting enzyme (ACE) inhibitory ac- sume 4.5 g lactoferrin. After conclusion of the
tivity, which blocks the conversion of angiotensin study, results revealed bovine lactoferrin survived
I to angiotensin II, a highly potent vasoconstric- passage through the stomach, demonstrating the
tor molecule.38 beta-Lactoglobulin has been de- ability of this protein to survive in an acidic envi-
scribed by Nagaoka et al as a cholesterol-lower- ronment.44
ing agent. In animal studies, beta-lactoglobulin
inhibited cholesterol absorption by changing mi- Clinical Indications for Whey
cellar cholesterol solubility in the intestine.39
Cancer
Whey protein concentrates have been re-
Absorption and Digestion of Milk searched extensively in the prevention and treat-
Proteins: Casein versus Whey and ment of cancer. Glutathione stimulation is thought
Lactoferrin to be the primary immune-modulating mechanism.
The physical manifestations of milk in the In a review of whey protein concentrates in the
gut vary, based on the type of milk protein. Whey treatment of cancer, Bounous discusses the anti-
comprises approximately 20 percent of milk pro- tumor and anticarcinogenic potential. The amino
tein, while 80 percent of protein in milk is casein.32 acid precursors to glutathione available in whey
These two proteins possess quite different prop- might: (1) increase glutathione concentration in
erties. Casein proteins form curds in the stomach, relevant tissues, (2) stimulate immunity, and (3)
detoxify potential carcinogens.45 Other authors breast, one patient with pancreatic cancer, and one
conclude the iron-binding capacity of whey may with liver cancer.59 Patients were given 30 g whey
also contribute to anticancer potential, as iron may protein concentrate (Immunocal) for six months.
act as a mutagenic agent causing oxidative dam- In six patients, blood lymphocyte GSH was el-
age to tissues.46 evated initially, suggesting high tumor GSH lev-
Many animal studies have examined the els. After completion of the study, two of the pa-
effects of whey and its immune-enhancing com- tients showed signs of tumor regression and a re-
ponents, including lactoferrin and beta-lactoglo- turn of lymphocyte GSH levels to normal, while
bulin.47-54 In animal studies where colon cancer was two patients showed signs of tumor stabilization
induced, whey demonstrated significantly lower without normalization of glutathione levels. Three
incidence of tumors, as well as fewer aberrant patients had progression of disease and lympho-
crypts.47,49,52,54 When whey-based protein powders cyte GSH levels increased from initial measure-
were compared to soy-based protein powders, ments. The conflicting results and small size of
similar effects were also observed.48,52 Yoo et al this study indicate the need for a larger clinical
demonstrated lactoferrin has the ability to inhibit trial to investigate the potential of a whey protein
metastasis of primary tumors in mice with can- concentrate singly and as an adjunct to chemo-
cer.50 Bovine serum albumin (10-15 percent of total therapy.
whey protein) has demonstrated inhibition of In a recent clinical trial, 20 patients with
growth in human breast cancer cells in vitro.55 A stage IV malignancies (one bladder, five breast,
hamster study demonstrated fractionated whey has two prostate, one neuroblastoma, one ovarian, one
the ability to prevent and treat 5-fluorouracil che- gastric, three colon, one mesothelioma, two lym-
motherapy-induced oral mucositis.56 This protec- phoma, two non-small cell lung, and one osteosar-
tion is thought to occur via induction of tumor coma) received a combination of 40 g/day non-
growth factor-beta (TGF-β), which reduces basal denatured whey protein concentrate, 50-100 g/day
epithelial cell proliferation. intravenous Transfer Factor Plus® (a supplement
A recent in vitro study by Kent et al dem- containing several immunoactive components), 1-
onstrated that an isolate of whey protein, when 2 g/day oral ascorbic acid, Agaricus blazei tea, a
compared to a casein-based protein, increased glu- multiple vitamin/mineral complex, 500 mg
tathione synthesis and protected human prostate Andrographis paniculata twice daily, and a soy
cells against oxidant-induced cell death.57 An in extract for six months.60 After six months there
vitro study on a human hepatoma cell line was were 16 survivors, all of whom had significantly
conducted using a high lactoferrin whey concen- higher NK function and higher mean hemoglobin
trate (Immunocal®), a baicalein medium, or a com- and hematocrit levels. All patients noted having
bination of the two.58 Baicalein, a potential anti- an improved quality of life during the course of
cancer drug, is a flavonoid extracted from the study. No comparison was made between this
Scutellaria revularis that is thought to have anti- combination therapy and whey protein alone.
tumor activity. Immunocal alone did not have a
significant impact on the hepatoma cell line. How- Hepatitis
ever, when Immunocal was combined with Whey protein supplementation demon-
baicelein, cytotoxicity was enhanced by inducing strates variable effects in patients infected with
a higher rate of apoptosis than in the group treated Hepatitis B or C. Initially it was found that bo-
with baicalein alone. vine lactoferrin prevented hepatitis C virus (HCV)
To date, few clinical trials on whey and infection in vitro in a human hepatocyte line.61
cancer have been conducted. It has been proposed These results prompted further clinical trials.
that GSH concentrations are high in tumor cells, A pilot study was conducted on 11 patients
giving them resistance to chemotherapeutic with chronic HCV. Each patient received either
agents.59 In 1995, a small trial was conducted on 1.8 or 3.6 g bovine lactoferrin daily for eight
five patients with metastatic carcinoma of the
against Pseudomonas aeruginosa, Listeria signaling molecule for initiation of protein syn-
monocytogenes, and E. coli.32,80 In addition to the thesis.8,89 It has been speculated that the quality of
above-mentioned bacteria, lactoferrin has also a particular protein for enhancing muscle hyper-
demonstrated antifungal activity toward Candida trophy and strength is related to its leucine con-
albicans.81 tent.36 The amino acid profile in dietary proteins
influences their nitrogen utilization, and poor qual-
Cardiovascular Disease ity dietary proteins have been shown to increase
In recent years, studies have linked a high- nitrogen losses and limit protein synthesis.90,91
fat diet to an increased risk of cardiovascular dis- Human studies documenting the benefi-
ease (CVD). Because CVD is linked to a number cial effect of whey protein supplementation on
of other factors, including increased age, genet- muscle size and strength are limited. Burke et al
ics, obesity, sedentary lifestyle, and alcohol intake, demonstrated that men engaged in resistance train-
quality of dietary fat must be taken into consider- ing programs while supplementing with whey pro-
ation. Milk is composed of more than 12 different tein concentrates showed greater improvements
types of fat, including sphingolipids, free sterols, in strength than men using resistance training
cholesterol, and oleic acid.82 Several studies have alone.92 Forty-two men, ages 18-31, familiar with
found milk intake and milk products lower blood weight training, followed the same high volume,
pressure and reduce the risk of hypertension.83-85 heavy load, and free-weight resistance-training
A study was conducted on a group of 20 program for 12 weeks. The participants were di-
healthy adult males to investigate whether a fer- vided into three groups in double-blind fashion:
mented milk supplement with an added whey pro- whey protein (1.2 g/kg body wt/day), a multi-in-
tein concentrate would affect serum lipids and gredient whey protein sports supplement (1.3 g/
blood pressure.86 The fermented milk contained kg body wt/day), or placebo (1.2 g/kg body wt/
both Lactobacillus casei and Streptococcus day of maltodextrin ). At baseline there were no
thermophilus. During the course of eight weeks, significant differences among the groups in lean
volunteers consumed 200 mL of fermented milk tissue mass or strength as determined by four
with whey protein concentrate or a placebo in the measurements, including bench press, squat,
morning and evening. The placebo consisted of a isokinetic knee extension, and isokinetic knee flex-
non-fermented milk product without the addition ion. After 12 weeks men who received whey pro-
of whey protein concentrate. After eight weeks, tein or the multi-ingredient whey protein sports
the fermented-milk group showed significantly supplement in combination with resistance train-
higher HDLs and lower triglycerides and systolic ing showed greater improvements in one of four
blood pressure than did the placebo group. While muscle strength measurements. In addition, whey
total cholesterol and LDL levels were lower in the supplemented groups showed greater improve-
fermented-milk group, the difference was not sta- ments in lean tissue mass over the placebo group.
tistically significant. While this study does not demonstrate whey
protein’s superiority over other protein sources, it
does show improvement in resistance training with
Exercise the addition of protein supplementation.
Whey protein supplements, including pu- Lands et al showed the effect of three
rified alpha-lactalbumin sports drinks, have been months whey protein supplementation (10 g twice
readily utilized in the consumer market because daily) versus the same amount of casein as placebo
of the high protein quality score and high percent- on muscular performance in 18 men.93 At baseline
age of BCAAs.87 Whey proteins can contain up to there were no significant differences in age, height,
26-percent branched chain amino acids,2,88 which weight, percent ideal body weight, whole leg
are efficient substrates for synthesizing new pro- isokinetic cycle testing, peak power, or 30-second
teins. For example, the BCAA leucine acts as a work capacity. Peak power and 30-second work
The authors state 400-800 mL of milk is equiva- Whey provides protein necessary for wound heal-
lent to the 40 mg dose of MBP. More recently, ing. Surgery stresses the body, altering natural
Yamura et al conducted a similar double-blind defenses, leading to various post-surgical compli-
study examining 30 women over a six-month pe- cations. Zimecki et al demonstrated lactoferrin
riod. Results indicate a daily dose of 40 mg per regulated the immune response and provided pro-
day of MBP significantly increases radial bone tective measures in post-surgical patients.132
density.125 Human studies demonstrated whey pro-
tein improved cognitive function and coping abil-
Gastrointestinal Support ity in highly stressed individuals.133,134 A rise in
A small, randomized, double-blind, cross- serotonin is thought to improve adaptation to
over study was conducted on 10 children with stress,135 and the authors proposed the tryptophan
short bowel syndrome to examine the effect of a available in whey provides a substrate to increase
hydrolyzed versus a non-hydrolyzed whey protein brain serotonin levels. The treatment groups for
on growth and development.126 Energy intake, ni- both studies received alpha-lactalbumin-enriched
trogen balance, intestinal permeability, and weight whey protein because it has the highest tryptophan
gain were similar among all children, indicating concentration of whey protein fractions.
the particular form of whey was not an essential Table 5 summarizes the clinical trials us-
component. In children experiencing bowel resec- ing whey.
tion, food introduction and promotion of normal
growth and development is of utmost importance. Conclusion
The increased transit time of whey proteins in the Milk is one of the oldest functional foods
small intestine makes it an ideal protein source available to mammals. From birth, mammals rely
for this small subset of children. on mother’s milk for nutrition and immune pro-
Whey protein has demonstrated a protec- tection. Scientists are beginning to develop an
tive effect on the gastric mucosa. This effect is understanding of the various components of milk,
thought to be related to the sulfhydryl component, including whey, and how they may impact health
particularly cysteine and its link with glutamic acid and disease. Considerable positive research re-
in the production of glutathione.127,128 Several ani- garding whey and its biological components con-
mal studies have demonstrated this protective ef- tinues to be published. It is noteworthy some of
fect.129,130 In a study by Rosaneli et al, rats fed a the research has been funded by commercial dairy
whey protein concentrate showed a 41-percent organizations, although a significant amount of
reduction in ulcerative lesions caused by ethanol clinical data from Asia was not supported by dairy-
ingestion, while a 73-percent reduction rate was aligned interest groups.
observed following repeat doses of whey.129 A To date, no severe adverse reactions have
study by Matsumoto et al demonstrated an iso- been noted following administration of whey pro-
lated alpha-lactalbumin formula had a four-fold tein products, although some patients note minor
effect on reducing ulcerative lesions compared to gastrointestinal disturbances. For individuals with
a whey protein formula containing only 25-per- frank milk allergies, whey products may not be
cent alpha-lactalbumin.130 suitable, although many individuals sensitive to
dairy find that casein is the culprit and they can
Other Uses of Whey Protein tolerate whey. Other dairy-sensitive individuals are
The wide range of essential and non-es- lactose-intolerant. Most whey proteins are pro-
sential amino acids, minerals, fats, and biologi- cessed to remove lactose and finished whey prod-
cally active proteins in whey provide extensive ucts only contain trace amounts. De-lactosed
application in clinical nutrition. Adequate protein whey, produced from crystallizing a majority of
intake is essential for post-surgical wound heal- the lactose out and recovering the remaining whey,
ing and protein depletion delays healing time.131 is appropriate for lactose-intolerant individuals.
A challenge test with a small portion of a particu- foods will develop. In addition to the isolated
lar whey supplement for an individual with dairy colostrum, lactoferrin, and alpha-lactalbumin
sensitivities would be indicated before beginning products currently available, glycomacropeptide,
therapeutic amounts. lactoperoxidase, whey immunoglobulin, and
As researchers further investigate whey bovine serum albumin isolated products may be
components it can be expected that new functional investigated.
Currently the variety of whey proteins 7. Daenzer M, Petzke KJ, Bequette BJ, Metges
available allows for tailoring their use for specific CC. Whole-body nitrogen and splanchnic
amino acid metabolism differ in rats fed mixed
clinical indications. Hydrolyzed whey provides
diets containing casein or its corresponding
readily available di- and tri-peptide fractions at- amino acid mixture. J Nutr 2001;131:1965-
tractive to athletes and other individuals desiring 1972.
a quickly absorbed, low allergenicity protein 8. Anthony JC, Anthony TG, Kimball SR,
source. Undenatured whey provides the highest Jefferson LS. Signaling pathways involved in
concentrations of intact native proteins such as translational control of protein synthesis in
lactoferrin and immunoglobulins for immune skeletal muscle by leucine. J Nutr
2001;131:856S-860S.
modulation. This is attractive to practitioners us-
ing whey as a functional food for immune-com- 9. Pierce A, Colavizza D, Benaissa M, et al.
Molecular cloning and sequence analysis of
promised patients and as an antimicrobial agent. bovine lactotransferrin. Eur J Biochem
Glycomacropeptide isolates do not contain the 1991;196:177-184.
amino acids phenylalanine, tryptophan, or ty- 10. Steijns JM, van Hooijdonk AC. Occurrence,
rosine, providing a valuable protein source for structure, biochemical properties and techno-
individuals with PKU. logical characteristics of lactoferrin. Br J Nutr
Because of the wide variety of whey prod- 2000;84:S11-S17.
ucts, practitioners using whey could benefit from 11. Levay PF, Viljoen M. Lactoferrin: a general
obtaining an analysis of whey proteins being rec- review. Haematologica 1995;80:252-267.
ommended to patients. Helpful information for 12. Bonang G, Monintja HE, Sujudi, van der
deciding whether a particular whey protein is ap- Waaij D. Influence of breastmilk on the
development of resistance to intestinal
propriate for individuals includes total protein colonization in infants born at the Atma Jaya
concentration and percent beta-lactoglobulin, al- Hospital, Jakarta. Scand J Infect Dis
pha-lactalbumin, glycomacropeptides, immuno- 2000;32:189-196.
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In addition, the presence of fat, lactose, and min- clinical uses. Altern Med Rev 2003;8:378-394.
erals should be considered. 14. Kulczycki A Jr, MacDermott RP. Bovine IgG
and human immune responses: Con A-induced
mitogenesis of human mononuclear cells is
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