CONTENT NAME OF THE ORGANISATION REPUBLIC OF TURKEY MINISTRY OF AGRICULTURE AND RURAL AFFAIRS ADANA FOOD CONTROL LABORATORY

LOCATION OF THE ORGANISATION Adress Phone Fax Web E mail : Kprl mah. 01230 Yreir ADANA : 0 322 344 19 19 : 0 322 344 17 67 : 01kontollab.kkgm.gov.tr : 01kontrollab@kkgm.gov THE BRIEF HISTORY OF INSTITUTION Adana Provincal Control Laboratory Directorate was established in 1987. During institution, 3 rooms of the top flor become active as the Directorate of Cotton Breeding Station at Kprl with manager, 4 engineers,2 technicians and 2 administrative staff in agricultural campus. Initially , there is no laboratory unit therefore the administrative structure provided without laboratory services . Then by doing internal arrangements in garden of Adana Vocational School for Agriculture was made laboratory with prefabricated buildings. The laboratory chief of the Adana Animal Diseases Research Institude ,settled in Kurttepe, provide transfer of device and chemical substances to our directorate. Analysis of food and feed started 45 days after moving. In 1991 the protocol signed between our directorate and the Directorate of Cotton Breeding Station. Revolving Fund Management Directorate of the ownership of the Cotton Breeding Station Adana Kprl neighborhood of the district administration building (two flor) and the laboratory building Adana Provincial Control Laboratory Directorate have been transferred to the order only to be given the right to use. The two storied building located on the ground used as a museum of agriculture department was transferred into the laboratory with renovations and regulations. In 1992 , February, administrative section of the directorate and Physical Analysis Laboratory , Additive Analysis Laboratory were moved to this building . In 1993, Chemical Analysis Laboratory moved to additional laboratory building. In 2000, maintanance and repairing of administrative building of our directorate and air conditioning of all units was provided. In 2001, Microbiology Laboratory ; in 2002 Physical Analysis Laboratory were restored. In 2003 Additive Analysis Laboratory and Mycotoxin Laboratory was carried out with renovation and repairing. In 2004, 32 units of laboratory equipment has been allocated to our directorate under the MEDA Project. In 2006, 2 Gas Chromotography (GC) and Gas Chromotography with a Mass Spectrophotometer (GC MS) were taken by the Govenorship of Adana Province Administration Directorate under the Monitoring of Drug Residues in Foods Agricultural Project. Cotton Breeding Station owned by Directorate of Adana in 2007, the unused building of Breeding Station Laboratory has been transferred by a protocol to laboratory directorate with repairing and regulations building work up into laboratory and has started to work for Sample Admission and Adjustment and Mineral Analysis Laboratory. Our directorate is working by following all kinds of national and international developments. At the same time in order to ensure international acceptability and validity of the experiments performed on a product basis , national and / or international Standard methods accredited by TURKAK on 21.11.2007 has been working every year by expanding the scope. The main service units: 1- Sample Admission and Adjustment 2-Physical Analysis Laboratory 3-Additive Residue Analysis Laboratory 4-Chemical Analysis Laboratory 5-Microbiology Laboratory 6-Mineral Analysis Laboratory 7-Biosafety Analysis Laboratory

PURPOSE OF SUMMER PRACTICE Purpose is understanding how theoretical knowledges are acted practically and having experience at application areas. Also it is gaining technical knowledge-talent, practical knowledge-talent additional to knowledge learning from university. SCOPE OF SUMMER PRACTICE 1.Laboratory analyses 2.Production scale

MANAGER
INTERNAL RESEARCH UNIT

ASSISTANT MANAGER

QUALITY CONTROL MANAGEMENT

UNIT OF MEASUREMENT UNCERTAINTY CALIBRATION UNIT

TRAINING OFFICER

EXECUTIVE FINANCIAL WORK

UNIT OF EUROPEAN UNION

SAMPLE ADMISSION AND ADJUSTMENT

PHYSICAL ANALYSIS LABORATORY

CAPITAL STOCK ACCOUNTING

ADDITIVE AND RESIDUE ANALYSIS LABORATORY

CHEMICAL ANALYSIS LABORATORY MINERAL ANALYSIS LABORATORY

INTERNAL

CONTROL UNIT

BIOSAFETY ANALYSIS LABORATORY MICROBIOLOGY ANALYSIS LABORATORY

ADMINISTRATOR ASSISTANT ADMINISTRATOR FOOD ENG. 6 CHEMIST 1 AGRICULTURAL EN. 16 BIOLOGIS T 3 CHEMICAL ENG. 1 ENG. OF FISHERIES 2 LAB. TECH. 4

VETERINAR TECHNICIA Y N 1 1 WORKER 14 TOTAL 53

OFFICER 2

Control of samples and documents brought Admission and storage of sample Wage determination and payment Document register Manager approval Referring SAA Registration of sample acceptence book Registration and of sample tracking system Delivering of sample to laboratory with sample delivery form Transport of sample Delivering to laboratory
NOT ACCEPTABLE

Control of sample at laboratory

Giving back to SAA nforming customer

Analyzing and storage under apropriate conditions Sample remained Delivering of sample requested

RESOLVED

Resolve the problem Giving back sample

15 days storage

Destruction of sample

ACCEPTANCE OF SAMPLE AND ARRANGEMENT REPORT

FUNCTION OF QUALITY MANAGEMENT UNIT AND STRUCTURAL FORM 1. Quality Management Unit provides documantation of good laboratory applications for registration, instruction, research, development, method, analysis of results, to control of the devices about reliabilities, to follow calibration of the devices, system, program and procedures. 2. A laboratory manual which is includes quality system purpose and targets, function and responsibilities, layot, facility and equipments, required datas about staff is prepared by Quality Management Unit. 3. All documents have to be clear, reachable applicable. These document must reach the target staff members. 4. Unit appoints persons who know evaluating the results of tests, purpose of the tests, methods of the tests and procedures. 5. Unit keeps a document about methods, procedures, results and purposes of anaylsis. Moreover, these documents are updated bye unit. 6. Unit offers new projects which is include improvement of staff to the Director. It prepares standard operating procedures for quality management unit. 7. Directly connected to the Ministry of laboratory directorates,by the Ministry creation of two deputy directors, if deemed appropriate, quality management unit,headed by deputy technical manager consists of at least three people. 8. This person must have worked in the laboratory for at least five years. QualityManagement Unit which trained people and quality management issues need to be trained as chefs in laboratory. 9. The only laboratory assistant in the offices of the administration sees fit, and the above-mentioned features of the Quality Management Unit which consists of at least three people and responsible to the Director. Laboratory is managed by directors and staff. But they have rules, procedures and standards which is include methods and management of analysis. Quality of standard is named as ISO/IEC 17025. ISO/IEC 17025 is the main standard used by testing and calibration laboratories. Laboratories use ISO/IEC 17025 to implement a quality system aimed at improving their ability to consistently produce valid results. It is also the basis for accreditation from an Accreditation Body. Since the standard is about competence, accreditation is simply formal recognition of a demonstration of that competence. A prerequisite for a laboratory to become accredited is to have a documented quality management system. The usual contents of the quality manual follow the outline of the ISO/IEC 17025 standard.

CONTENTS OF THE ISO/IEC 17025 1) Scope 2) Normative references 3) Terms and definitions 4) Management Requirements i) Organization ii) Managemet Systems iii) Document Control iv) Review of requests, tenders, and contracts v) Subcontracting of tests and calibrations vi) Purchasing services and supplies vii) Service to the customer viii) Complaints ix) Control of noncomforming and/or calibration work x) Improvement xi) Corrective Action xii) Preventive action xiii) Control of records xiv) Internal audits xv) Management review 5) Technical requirements i) General ii) Personnel iii) Acommodation and enviromental conditions iv) Test and calibrations methods and method validations v) Equipment vi) Measurement traceability vii) Sampling viii) Handling of test and calibration items ix) Assuring the quality of test and calibratio results x) Reporting the results

ISOLATION OF DNA FROM FOOD AND FEED

A genetically modified organism (GMO) or genetically engineered organism (GEO) is an organism whose genetic material has been altered using genetic engineering techniques. These techniques, generally known as recombinant DNA technology, use DNA molecules from different sources, which are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel genes. Transgenic organisms, a subset of GMOs, are organisms which have inserted DNA that originated in a different species.In this analyss DNA exraction was applied by using canola.

ekil 1 seed canola PROCEDURE

Homogenized about 0.2 gr material with a commercial immediately before use. lysis

homogenizer.Preheated lysis buffer to 65

Transfered the resulting powder to a 2 ml tube and added 550 buffer CF.Mixed carefully, added 10

proteinase K and mix again.

Incubated at 65 for 30 min. Afterwards,cetrifuge the mixed for 10 min (>10.000xg) to pellet contaminants and cell debris. Pipette 300 300 clear supernatant into a new 1.5 ml centrifuge tube.Added ethanol.Vortexed the mixture for 30 sec.

buffer C4 and 200

Placed a GENESpin column into a new 2 ml centrifuge tube and pipette 750 mixture onto the column.Centrifuged for 1 min at 10,000xg.Discarded flow-through.

Pipette 400 buffer CQW onto the GENESpin column.Centrifuged for 1 min at 10,000xg.Discarded flow-through. Pipette 700 buffer C5 onto the column.Centrifuged for 1 min at 10,000xg.Discarded flow-through. Pipette another 200 buffer C5 onto the GENESpin column.Centrifuged for 2 min at full speed (10-16,000xg) in order to remove buffer C5 completely. Place the GENESpin column in a new 1.5 ml centrifuge tube.Pipette 100 elution buffer CE onto the membrane.Incubated for 5 min.Centrifuged for 1 min at full speed to collect the DNA.

PREPARATION OF THE PCR MIXES MIX FOR PLANT GENE-PCR COMPONENT Plant Gene Master Mix Enzyme Solution Internal Control TOTAL VOLUME VOLUME 18 1 1 20

1.Thawed the solutions and, for maximal recovery of components, briefly spined vials in a microcentrifuge before opening.Mixed carefully but thoroughly by pipetting up and down. 2.In a 1.5 ml reaction tube, prepared the PCR Mix by adding the following components in the order mentioned below,then mixed gently but thorougly by pipetting up and down. 3.Mixed carefully but thorougly by pipetting up and down.Did not vortex

Pipet 20

PCR was mixed in to each well

For the samples of interest,added up to 5 added H2O to 5 )to a well H2O

sample DNA (f less than 5

For the negative control,added 5 Fort he positive control, added 5 Template

foodproof GMO Screening Control

4.Sealed the plate accurately with an optical sealing foil 5.Placed the plate in a swing bucket cetrifuge and centrifuged at 1,500xg for 30 s. RESULT; There were found enough DNA DNA CONCENTRATION 5.86 ng/ , DNA was diluted with 0,2 for PCR

MINERAL ANALYSIS LABORATORY Minerals are essential for a healthy life. They are required to live without the body functions not remain in a healthy way. Minerals are inorganic substances the body can not make. There are many minerals that are vital to our health more than 15. Mainly vitamins, minerals, vitamins work together to provide access to the region in the greatest need. For minerals, vitamins work the same way. Cell protection and healthy teeth, bones, skin structure is important for. Minerals as well as blood pressure, heart rhythm, muscle function, fluid balance in the body housing, breeding, and many more play an important role in the function. Scientific studies have shown that the lack of mineral loss, and directly affects our health. Some manufacturers have resorted to tricks we had to get minerals from the outside so mneral analyss are appled n ths laboratory.

DETERMINE THE AMOUNT OF SELENIUM IN THE EGG Mineral Selenium - Selenium Benefits and Functions: A powerful antioxidant, selenium strengthens the immune system and reduces the risk of cancer. Protects the cells and slows the aging process. Tissue, increasing the flexibility and helps to protect heart cells, supporting the heart and vascular health. Sperm production and viability, including in particular, plays a role in reproductive health. Reduce the harmful effects of toxic substances in the body. Activities in the liver contributes to maintain a regular basis. Our aim is to determine amount of selenium in egg by using ICP-0ES machine in the mneral analyss laboratory. MATERIALS AND CHEMICALS; sensitive scale,blender, Teflon tube for combustion, graduated flask, ashless filter paper, 35 mm or 45 mm diameter glass funnel, test tubes, fume hood, microwave sample preparation unit, ICP-OES machine HNO3 (%65), ultra distilled water, standard preparing solution. PROCEDURE Mixed sample by using mixer and made it homogeneous Taken 1 gr homogeneous egg and put in to teflon tube for combustion

Added 5 ml HNO3 in to teflon tube for combustion,waited for 20 min on the fume hood After that time, tubes were placed in to microwave sample preparation unit This process was taken 1 hour and after this process,sample was taken for fume hood, opening the cover of the pressure inside the tube is reduced balloon flask was transferred to the contents of containers cooled with the aid of a funnel Diluted with ultra distilled water Prepared sample was put in to test tube and placed ICP-OES mechine

RESULT; Amount of analyss(ppm)=Amount of Concentraton in to ICP-AXIAL X Diluted Factor /1000 SELENIUM = 0,623 ppm

ADDITIVE AND RESIDUE ANALYSIS LABORATORY Today, agricultural products, harmful insects, weeds and pathogenic organisms in order to maintain and increase agricultural yield to chemical control has become an inevitable requirement. Pesticide-called pests by eliminating these substances in food or food substances to increase production while providing significant levels of lead contamination. Excessive use of pesticides by producers with higher-quality product could be more because of the consumption of large amounts of medicine in question. After which levels of pesticide residues in foodstuffs in general are known to affect. However, close to the harvest period and during harvest as a result of the spraying of pesticide residues in increasing quantities. Therefore, high levels of pesticide residues in foodstuffs may exist from time to time. Therefore, the initial phase of pesticides and chemical warfare method was seen as a savior in the health and environmental problems caused by the later emerged. Today has reached levels of pesticide residues to human health threat. Therefore, determination of pesticide residues in food is a lot of food raw materials and finished products has become imperative. Organic Phosphorus and nitrogen in our laboratory group with the group of chlorinated organic pesticide analysis is done. PESTCDE N APPLE,CRUET,ONION,CUCUMBER Pesticides are substances or mixture of substances intended for preventing, destroying, repelling or mitigating any pest.A pesticide may be a chemical substance, biological agent (such as a virus or bacterium), antimicrobial, disinfectant or device used against any pest. Pests include insects, plant pathogens, weeds, molluscs, birds, mammals, fish, nematodes (roundworms), and microbes that destroy property, spread disease or are a vector for disease or cause a nuisance. Although there are benefits to the use of pesticides, there are also drawbacks, such as potential toxicity to humans and other animals. According to the Stockholm Convention on Persistent Organic Pollutants, 9 of the 12 most dangerous and persistent organic chemicals are pesticides. Thus we observed pesticide in apple,cruet,onon,cucumber. In this experiment, we used blender, micro pipette, vial, centrifuge, GC-MS,LC- MS/MS, plastic or teflon tubes , sensitive scales REAGENT; MgSO4 , PSA, SODIUM ACETATE, %1(%1 ACETIC ACID %99 ACETONITRILE) PROCEDURE Prepared plastic tubes by weighing 6 gr MgSO4 and 1,5 gr sodum acetat and 0,6 gr MgSO4 and 0,2 gr PSA Grinded sample by usng blender and weighed 15gr sample Added 15 ml %1 acetic acid soluton in to sample Mixed with vortex for one mnute Centrifuged at 5000 rpm turnover

Taken 4 ml soluton from above centrifuged soluton and put in to plastic test tube that contans 0,6 gr MgSO4 and 0,2 gr PSA Mixed with hand or vortex Centifuged at 5000 rpm turnover Taken soluton from tubes by usng mcropipette and put in to vials Put in to GC-MS and LC-MS/MS

RESULT; SMALLER THAN REPORTED LIMIT