Proximate analysis of chlorella speices cultured before growth of chlorella species

Nutrient C. Protein C. Lipid C. FIbre Ash Moisture C NFC Nutrient values Tr1 T1R T2(10ml) 24.5 12.33 12.00 2.50 15.0 33.67 23.26 13.40 12.60 2.20 15.4 33.14 16.41 10.03 10.00 4.50 17.9 41.16 T2(10ml) 17.46 9.00 10.56 4.80 18.10 40.08 T3(mls) 12.51 9.50 14.00 7.50 22.08 34.41 T3R 11.79 9.0 11.00 9.0 20.20 39.01 C1 35.71 14.15 12.40 5.30 5.0 27.44 C2 (non) 35.09 13.25 16.00 5.60 5.5 24.56

Proximate analysis of chlorella species polluted after growth of chlorella species

Nutrient C. Protein C. Lipid C. FIbre Ash Moisture C NFC Nutrient values Tr1 T1R T2(10ml) 34.57 6.25 10.00 2.00 10.00 37.18 34.78 12.00 11.00 2.40 9.00 30.82 32.46 9.00 8.00 2.100 12.00 36.44 T2(10ml) 33.71 11.00 7.00 2.50 10.00 42.29 T3(mls) 23.09 10.00 7.00 4.00 13.00 42.91 T3R 17.66 11.00 5.00 3.90 11.00 51.44 C1 37.83 12.00 13.00 5.00 6.00 26.17 C2 (non) 37.07 11.50 12.60 6.0 7.00 25.83

Footnote: C Crude, Nfe- Nitrogen Free extractive T1 = Treatment I, TIR Treatment I Replicate T2 Treatment 2, T2R Treatment 2 replicate T3 Treatment 3, T3R- Treatment 3 replicate

REFERENCES Asada K. (1999). The water cycle in chloroplast scavenging of active oxygen and dissipation of excess photons. Annu. Rev. Plant physiol. Mol. Biol. 50: 601-639. Lahaye, M. (19991). Marine Algae as source of fibres Determination of soluble and insoluble dietary fibre content in some vegetable. J. Sci. Food Agric 54:587594. Bilanovic, D., Sukenik A., G, Shelef (1988). Floculation of micro algae with cationic polymers effect. Halama, D. (1990). Single cells protein in non-coonventual feedstuff in the nutrition of farm animals ed. Koloman Boda. Elsevier Science Publishing Company, Inc 655 Avenue of America New York. N.Y. 10010 pp 34-49. Gilbert, V., Levin, John R. Clendenning, Ahmon,. Gibor and Fredrick D. Bogar. (2006). Harvesting of Algae by froth floatation: Resources; Inc Washington Dc. Becher E.N. (1992). Micro algae for human and animal consumption pp 222-256. In M.A. Borowitzka and L.J. Borowitzka (ed) Micro algal biotechnology. Cambridge University Press. Cambridge united Kingdom. Borowitzka, M.A., and L.J. Borowitzka (ed). (1992). Microalgal biotechnology, Cambridge University Press, Cambridge, United Kingdom. De Pauw, N; and Persoone (1992). Microalgae for aquaculture 197-221. In M.A. Borowitzka and L.J Borowitzka (ed) micro algal biotechnology. Cambridge University Press. Cambridge United Kingdom. O Reilly, A.M., and J.A. Scott (1995). Defined Commobilization of Mixed Micro-Organism cultures, enzymes microb. Technol. 17: 636-646.

Oswald, W.J. (1992). Micro-algae and Waste water treatment pp 305-328. In M.A. Borowitzka and L.J. Borowitza (ed). micro algal biotechnology. Cambridge University Press. Cambridge United Kingdom. Bashan, Y. (1998). Inocculation of Plant growth promoting bacteria for use agriculture. Biotechnol. Adv. 16:729-770. Rouke, B. J. Tramper, Wijffels R.H (2003). Ultrasound, A New technique to harvest microalge Journal of Applied Phycology 15 (2-3) 143-153. Kayombo S. Mbwette T.S.A., Katima, J.H.Y and Jorgensen, S.E. (2003). Effect of Substrate Concentration on the growth of heterotrophic bacteria and algae in secondary facultative ponds water research vol. 37:12: 2937-2973. Zhang Xue-wu., Chenfeng and Johns, Micheal R.(1999). Kinetic Model for heterotrophic growth of clamydomonas reindardtic in batch and fed batch culture process. Biochemistry Vol. 35:3-4, 285-389.

Fiedler, E.J, Marcelo and Shelef G., Simulation model of waste stabilization reservoirs. Ecological Engineering, Vol. 20. No. 2: pp. 121-145.

water

El-Sheekh M.M., El Naggar, A.H. and Osman M.E.H (2000). Comparative Studies on the green algae chlorella homosphaera and chlorella vulgaris with respect to oil pollution in River Nile. Water Air Soil Pollution 124: 187-204. Richard, M. McCourt (1995). Trends in Ecology and Evolution pp. 159-163.

Xia, J., Gao K. (2003). Effect of double atmospheric Co2 concentration on the photosynthesis and growth of chlorella pyrenoidosa Cultured at varied levels of light. Fisheries science 69:769-771. Cohen, Z; Reunggitchachawli, Siangdung W,; Tantichareon, M; and Heimer Y. M; (1993). Herbicide resistant lines of microalgae. Growth and falty acid composition. Photochemistry. Xu, Ling (2009). Microalgal bio reactors: Challenges and opportunities. Engineering in life science 9 (3). Campo, Jose A. (2007). Outdoor cultivation of microalgae for caroterioid production, current state and perspective. Applied microbiology and bio technology. Loren, M., Friedle, T, and Day, J.G. (2005). Perpetual maintenance of actively metabolizing microalgal cultures. In Algal culturing techniques (Anderson R.A. ed). Academic Press, New York pp. 145-106. Day, J.G. and Brand, J.J (2005). Cryopreservation Method for maintaining cultures in algal culturing techniques (Anderson R.A. edu) Academic Press New York 165-187. Grout, B. (1995). Genetic preservation of plant cell invitro, springer lab manual. Spinger Verlag Berlin, Germany. Fleck R.A., Pickup R.W., Day, J.G., Benson E.E. (2006). Characterization of Cryoinjurt in Euglena Gracilis using flow eytomery and cryomicroscopy. Cryobiology 52:261-268.

Benson E.E., Brenner, D.H. (2004). Oxidative stress in the frozen plant a free radical points of view. In fuller B, Lane N and Bense E.E. (eds) life in a frozen State CRC pp205-242. Gross, E.M., Feldbaum and Graf A. (2003). Epiphytes Biomas and elemental composition on submerse macroplytes in shallow entrophic lakes. Hydrobiologia 506:583-589. Schrader, K.K. Nanyakkara, NPD, Tucker, C.S. Rimondo A.M. Ganzera M., and Schaneberg (2003). Novel denuative of 9-10 athnraquinone are selective algicide against the musty odor cyanobacterium oscillatoria pernotata Appl. Environment Microbiol 69:5319-5327. Young, P.K.K, F.T. Wong (2002). Mimosine, the Allelochemical from the legiuminous tree leucocephala selectively enhances cell proliferation in dinoglagellaet. Appl. Environ. Microbiol. 68:5160-5163. ]ogboghodo, I.A., Erebor, E.B, Osemurota, I. O., Isitekhale H. H. (2009). Environmental Monitoring and Assessment: Volume 96: no 1-3, pp. 153-161 (0).

ACKNOWLEDGEMENT I offer a sincere gratitude to God Almighty who made my Sojourn in Uniben a reality with success.

My special thanks to my parents Mr. and Mrs. J.O. Egede who have been my bedrock throughout my days in school not to talk of their love and care for me since birth. My unreserved thanks to my siblings, Andrew, Isioma, Amara, Chukwus who have been there for me from the beginning. To a special one who have always been available in times of need, I say thank you Nkechi. To all my friends, Austine, Micheal, Johnson, Iyabiyi, Ifeoma, Adesuwa, Jennifer, Amaka, Judge, Joy, Benjamin and many more, I say thanks for always been there for me. My profound gratitude also goes to all my lectures from through University of Benin especially Prof. U.J. Ikahtus, Prof. J.M. Olomu, Prof. J.O. Igene, Dr. M.A. Bamikole, Prof. S.O. Nwokoro, Dr. V.E. Ezechi, Dr. J.M. Omoyakhi, Mr. G.I.O Odafe, Dr. (Mrs.) Oyegun and my first supervisor Dr. S.E. Vaikosen (R.IP).

APPENDIX I POLLUTION AFTER GROWTH Weight of sample at harvest (wet basis) Treatment C1 T1 T2 T3 Treatment C1 T1 T2 T3 Treatment C1 T1 T2 T3 Treatment C1 T1 T2 T3 Readings (g) 3.69 6.82 4.36 3.18 Readings (g) 2.56 1.09 1.96 1.60 Readings (g) 2.56 1.09 1.96 1.60 Readings (g) 1.21 1.05 1.23 0.19 Replicate C2 Y1R T2R T3R Replicate C2 Y1R T2R T3R Replicate C2 Y1R T2R T3R Replicate C2 Y1R T2R T3R Readings (g) 2.51 6.90 4.00 3.29 Readings (g) 3.00 1.12 2.86 1.90 Readings (g) 3.00 1.12 2.86 1.90 Readings (g) 1.82 1.02 0.56 1.31

POLLUTION BEFORE GROWTH Treatment C1 T1 T2 T3 Readings (g) 3.46 79.51 58.83 42.59 Replicate C2 Y1R T2R T3R Harvest Weight of samples at harvest (Wet basis) Treatment C1 T1 T2 T3 Readings (g) 2.01 1.10 2.66 2.59 Replicate C2 Y1R T2R T3R Readings (g) 1.04 2.56 1.75 3.98 Readings (g) 4.51 76.07 63.74 30.30

Treatment C1 T1 T2 T3

Readings (g) 3.53 14.19 12.10 7.07

Replicate C2 Y1R T2R T3R

Readings (g) 4.59 10.62 15.33 9.70

APPENDIX II Analysis of variance Variance: Ash Source of variation Pollution Trtment Pollution. Trtment Residual Total d.f. 1 3 3 8 15 s.s 11.3906 37.5919 12.6469 1.9250 63.5544 m.s 11.3906 12.5306 4.2156 0.2406 v.r 47.3 4 52.0 8 17.5 2 F.pr <.001 <001 <.001

Analysis of variance Variance: CrdFibre Source of variation Pollution Trtment Pollution. Trtment Residual Total d.f. 1 3 3 8 15 s.s 38.938 54.777 16.241 14.397 124.352 m.s 38.938 18.259 5.414 1.800 v.r 21.6 4 10.1 5 5.41 4 1.80 0 F.pr 0.002 0.004 0.095

ANALYSIS OF VARIANCE

Analysis of variance Variance: Ash Source of variation Pollution Trtment Pollution. Trtment Residual Total d.f. 1 3 3 8 15 s.s 3.4.32 21.87 16.461 21.914 63.494 m.s 3.432 7.229 5.487 2.739 v.r 1.25 2.64 2.00 F.pr 0.295 0.121 0.192

Analysis of variance Variance: Ash Source of variation Pollution Trtment Pollution. Trtment Residual Total d.f. 1 3 3 8 15 s.s 116.5320 275.1501 69.3401 5.2322 m.s 11.3906 12.5306 4.2156 0.2406 v.r 47.3 4 52.0 8 17.5 2 F.pr <.001 <001 <.001