November | December 2011 Feature title: Application of Enterococcus faecium as probiotic strain in aquatic species

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F: Enterococcus faecium
Experimental group Day 1 : Intestine with faecal materials

F: Enterococcus faecium
Table 1: E. faecium in tilapias intestine and faeces at day one and day 10 after stopping to feed probiotics including this probiotic strain E. faecium in tilapias intestine (x 108 cell/g intestine) Day 10 : Faecal material : Intestine w/o faeces

Control group Probiotic group

0 1.37 0.85

0 1.52 0.98

0 1.33 0.28

Application of Enterococcus faecium as probiotic strain in aquatic species

by Elisabeth Mayer MSc, Biomin, Austria

otics producing lactic acid (such as E. faecium) can provide a performance benefit are improving intestinal microbial balance (Fuller, 1989), stimulating the immune system and decreasing pH as well as the release of bacteriocins (Rolfe, 2000). Bacteriocins are small peptides which are characterized by their ability to inhibit pathogenic bacteria; whereas, some have a

ith the rapid developments in aquaculture fish and shrimp farming industries are constantly under threat due to the outbreak of infectious diseases.

Members of the genus Vibrio and Aeromonas have been described as being among the most common pathogenic species in shrimp and fish causing serious losses in larval and growout phases throughout the world. Use of antibiotics to control these agents has led to problems of drug resistance and resulted in trade restrictions in export markets. Picture 1: Microscopic As an alternaPotential picture (1000-fold tive strategy to probiotic magnification) of Enterococcus faecium these antimicrocandidates bial compounds, Lactic acid the prophylactic bacteria (LAB) use of beneficial bacteria (probiotics) has are potential probiotic candiemerged to improve health and zootechnidates in aquaculture and are cal performances such as survival, producalso known to be present in tion, feed conversion the intestine of healthy fish. and growth rates Enterococcus faecium is one of cultured aquatic of the most commonly used species. lactic acid producing bacteria Probiotic bacteria in animal nutrition and has are a sustainable approach to modulate become a focus of attention the gut microflora towards a favorable for use in commercially farmed composition, which is of utmost imporaquatic species.

tance since the digestive system of fish and shrimp is an important entrance point for infections. Hence, selection criteria of probiotics for aquaculture should be based on their antagonism towards pathogens (through competitive exclusion), their growth, attachment to intestinal mucus and production of beneficial compounds (Vine et al., 2004). Since fish and shrimp are cultured under different conditions the optimum temperature range should also be considered for selection of the right probiotic strain. Another important factor is that probiotics always lack pathogenicity. After all, probiotics should contribute to efficient production in a sustainable way, promoting healthy and robust animals (Brittain et al., 2002).

Enterococci are Gram-positive, facultative anaerobic bacteria which are widely distributed in nature and considered as bacteria of low pathogenicity (Klare et al., 2003). Probiotic enterococci infections have not been reported in the veterinary medicine, so the risk appears to be limited (Rinkinen et al., 2003). Eaton and Gasson (2001) found that E. faecium strains were also generally free of virulence determinants. Concerning possible acquired antibiotic resistances, testing the antibiotic profile of enterococci used as probiotics is of relevance. However, these antibiotic resistances are species- or genus-specific properties (Klare et al., 2003). Once the probiotic enterococci are accurately identified, they can be tested for specific resistances. Several important ways in which probi-

Picture 2: Microscopic pictures (1000-fold magnification) of Vibrio harveyi, Yersinia ruckeri, Streptococcus agalactiae and Aeromons veronii

narrow spectrum of activity while others inhibit a wide variety of bacteria. The use of the bacteriocin-producing E. faecium with probiotic properties alone or in combination with other beneficial intestinal bacteria was investigated in different studies. In vitro studies using the agar spot method (Rosskopf, 2010) have shown that Enterococcus faecium (strain IMB 52) has inhibition properties against a wide spectrum of aquatic pathogens including Yersinia ruckeri, Vibrio harveyi, Streptococcus agalactiae and Aeromonas veronii. Similar observation was made by Swain et al. (2009) who proved the inhibitory activity of E. faecium isolated from brackishwater fish against V. harveyi and V. parahaemolyticus. This demonstrates the potential applications of E. faecium from fish intestine for controlling pathogenic vibriosis in shrimp culture. It has also been reported that survival rates of European eels (Anguilla Anguilla L.) fed with E. faecium were significantly higher than in the control groups after challenged with Edwardsiella tarda (Chang and Liu, 2002).

Wang et al. (2008) demonstrated that the addition of E. faecium (1 x 107 CFU/mL) in aquaria water could significantly increase final weight and daily weight gain (DWG) of tilapia. Since certain immunological parameters (myeloperoxidase and respiratory burst activity) of tilapia were improved as well, the increased growth performance might be attributed to less bacterial challenge, confirming the benefit for the non-specific innate immunity of this kind of fish. Panigrahi et al. (2007) examined immune modulation including cytokine gene expressions of rainbow trout (Oncorhynchus mykiss) and demonstrated that these parameters were improved by probiotic feeding of freezedried Lactobacillus rhamnosus, Enterococcus faecium or Bacillus subtilis (109 CFU/g) after 45 days. Particularly the fish fed the E. faecium strain showed better performance which could possibly be linked to the suitable ambient temperature conditions of this strain. Temperature is a major environmental factor controlling microbial growth and the ideal conditions differ among microorganisms. E. faecium was found to be more psychrotolerant than the other two bacteria, growing well at temperatures ranging from 12 to 30 C. Rosskopf (2010) showed that the optimum temperature range for E. faecium (strain IMB 52) reaches as far as to 37 C. Using the fluorescence in situ hybridization (FISH) technique, Supamattaya et al. (2005, 2006) have demonstrated in a series of in vivo studies that E. faecium (strain IMB 52) is also able to populate the intestine of white shrimp (Litopenaeus vannamei) and Nile tilapia (Oreochromis niloticus) (see Picture 3 and Table 1) and induce a positive impact on bacterial ecology of the gut by inhibiting Vibrio spp. through competitive exclusion.

E. faecium was even detected in the fish gut and faeces 10 days after product administration. In addition, it was observed that dietary application of E. faecium (strain IMB 52) alone or in combination can improve growth performance, enhance the immune response (by increasing granular hemocytes level) (Supamattaya et al., 2005) and increase survival rate in shrimp contaminated with Vibrio parahaemolyticus (Krummenauer et al., 2009). Means SD. Using Fluorescent in situ hybridization (FISH), 5 - 15 microscopic fields were counted. From these studies it was concluded that Enterococcus faecium (strain IMB 52) is a safe and promising probiotic candidate for aquatic species which can be applied in different combinations.

Conclusion
Maintaining the balance of critical parameters and effective disease control remain fundamental requirements for successful aquaculture. In order to withstand the high stocking densities in shrimp and fish production probiotics are a promising feed additive to stimulate animal growth and advance disease resistance. Enterococcus faecium as probiotic strain in aquatic species is increasingly recognized as safe and can be applied in different combinations. E. faecium can grow at a wide range of temperatures and thus has an advantage over other bacteria considering that fish and shrimp are reared at different temperatures and conditions. The current paper illustrated with in vitro studies and performance trials the potential benefits of E. faecium as probiotic feed additive for fish and shrimp, either by stimulating the development of a healthy gut microflora or by inhibiting pathogenic bacteria like Vibrio spp., Yersinia spp. and Aeromonas spp.

References
Available on request

14 | InternatIonal AquAFeed | november-December 2011

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