Dr. Shakibaie MR C.V.

Personal Data
Name: Mohammad Reza
Surname/Family name: Shakibaie
Date of birth: 23/8/1967
Place of birth: Kerman/Iran
Nationality: Iranian
Sex: Male

Education
Ph.D: University Of Pune, India.
Years Attended: 1992-1997.
Major Fields Of Study: Molecular Bacteriology & Biotechnology.
Title Of Ph.D. thesis: Molecular Genetics Of Plasmid Mediated Silver &
Antibiotic Resistance in Acinetobacter baummannii
Postdoctoral Fellowship: Aquatic Microbial Lab. Mysore, India
Year Attended: 2006
Title: Horizontal transfer of antibiotic resistance genes among gram negative
bacteria in sewage and lake water and influence of some physico‐chemical
parameters of water on conjugation process

Employment
Permanent Position : Faculty Member/Associate Professor
Institute / University : Kerman University Of Medical Sciences
Department / faculty : Microbiology & Immunology
Current -position: Chairman of infectious diseases and tropical medicine
research committee/Kerman university of medical sciences, Kerman, Iran.
Current Research : Molecular Biology/Antibiotic Resistant genes/Biofilm
Tel: 0098‐341‐2457789
Fax :0098 341 3226171
Mobile: +989133408226
Academic E‐mail: mr_shakibaei@kmu.ac.ir
Personal E‐mail: mohammadreza.shakibaie@gmail.com
 Membership In Scientific Societies

Member, New York Academy of Science, science advisory board (USA)

Member, Asian ‐Pacific research foundation for infectious diseases
(ARFFID)
Affiliated member of European Society for Microbiologists (FEMS)
Member of Iranian Society of Microbiology
Editorial board World Academy of Sciences (Waset)
Editor of Int. J. Biol. Life. Sci.
Editorial team J Biotechnology and Molecular Biology Review
Editor of Current Research Journal of Biological Sciences
Editor J Bacteriology Research

The focal point of the research in our lab are plasmid mediated antibiotic and metal
resistance, Biofilms formation and its genes in hospital isolates of P.aeruginosa and
also enhancing bioremediation of heavy metals using genetic engineering organisms,
the enzyme that is responsible for the first step in gene expression, cloning some
genes responsible for Biofilms formation and also we are using genetic engineered
bacteria for decreasing the CO2 emission. All these steps are subject to elaborate
control by numerous regulatory proteins and small effectors.

M.Sc. Students thesis

1-Study on Curing of antibiotic resistance plasmids using Iranian plant extracts.

2-Study on Antibiotic sensitivity of pseudomonas aeruginosa isolated from burn
patients.
3-Study on Resistance of pseudomonas aeruginosa to chromium & copper and its
biotechnological implications.
4-Study on Antibiotic resistance, beta lactamase production and plasmid profile
of Neisseria gonorrheae strains.
 5-Study on isolation, species distribution, antibacterial resistance pattern and
Beta-lactamase production of enterococci isolated from human samples in
southeast of Iran.
6-Study on Myrtus extracts and its anti-supercoiling DNA (Co-worker).
7-Study on prevalence of Plasmids & its relation to Beta-lactemase in
Staphylococcus aureus.

8-Study on mechanism of metal resistance in Pseudomonas aeroginosa.

9-Study on antibiotic resistance pattern of Enterococci species isolated from

human and chicken alimentary channels.
10-Study on mechanism of Myrtus plant extract on supercoiling of Plasmid
PBR322 in E.coli k12 HB101.
11-Study on Plasmid mediated cefotaxime and ceftizoxime resistance in multiple
antibiotic resistance Klebsiella pneumonieae isolated from hospitals in Kerman,
Iran.
12-Study on antibiotic resistance, Beta-lactamase production, plasmid profile of
N.gonorrhoea isolated from patients with urtheritis.
13-Study on Prevalence of conjugative plasmids in E.coli strains isolate from
hospitals.
14-Study on detection of MBL/ESBL B-lactomases in Pseudomonas aeruginosa
isolated from burn patients in Kerman,Iran

15-Bioremediation of cadmium (Cd) using Cd resistant bacteria isolated from

activate sludge industrial waste effluent in batch bed reactor

16-Detection of SHV, PER, and TEM type of ESBL genes among Pseudomonas
aeruginosa isolated from burn patients in Shafa ‐ hospital Kerman

Number of Ph.D and M.Sc students working with me: At

present one PhD and one MSc.

Grants
1‐ Molecular profiling of ESBL genes of Pseudomonas aeruginosa isolated from burn
Patients using RFLP‐Sequencing techniques.

2‐ Study of antibiotic resistance and ESBL production in Acinetobacter strains

isolated from hospitals in Kerman, Iran.
Selected publications

1- Mohammad Reza Shakibaie et al. Antibiotic resistant pattern and ESBL production
among Acinetobacter spp. isolated from ICU of a hospital in Kerman, Iran.
Antibiotic resistance infection control (Biomedcentral), Accepted (2011).

2‐ K Mozafari Nia, G Sepehri, H Khatmi, MR Shakibaie. Isolation and antimicrobial

susceptibility of bacteria from chronic supportive otitis media patients in Kerman,
Iran. IRCMJ 2011 (Accepted).

3‐ Shakibaie MR and Moradie M. Detection of Carbenicillin hydrolyzing (CARB) type

of ESBL in hospital isolates of Acinetobacter baumannii. Int J Biol & Biomed Sci.
1(1):1‐6 (2011).

4‐ Fershteh Shacheraghi, Mohammad Reza Shakibaie, Hanieh Noveiri. Molecular

identification of ESBL genes blaGES‐1, blaVEB‐1, blaCTX‐M blaOXA‐1, blaOXA‐4,
blaOXA‐10 and blaPER‐1 in Pseudomonas aeruginosa isolated from burn patients
by PCR‐RFLP and sequencing techniques. Int J Biol & Life Sci. 6 (3): 138‐142,
(2010).

5‐ A. Jabbari Nezhad Kermani, M. Faezi Ghasemi A. Khosravan A. Farahmand and M R.

Shakibaie. Bioremediation of cadmium (Cd) using Cd resistant bacteria isolated from
activate sludge industrial waste effluent in batch bed reactor. Iran. J. Environ. Health.
Sci. Eng. 7(4): 279‐286
(2010).

6‐ Shakibaie M.R., Dhakephalker P.A., Kapadnis B.P., and Chopade B.A. Removal of
silver from Waste water effluents using Acenitobacter baumannii BL54. Can. J.
Microbiol. 45:995‐1000(1999). (37 citations) (book citation)

7‐ M.R. Shakibaie, K.A. Jalilzadeh and S.M. Yamakanamardi. Horizontal transfer of

antibiotic resistance genes among gram negative bacteria in sewage and lake water
and influence of some physico‐chemical parameters of water on conjugation
process J. Environ. Biol. 30(1):45‐49 (2009). (4 citations)
8‐ MR. Shakibaie, Pershant Dhakephalker, Balu Parnab Kapadnis, Balu Ananda
Chopade. Conjugational transfer and survival of plasmid encoding silver and
antibiotic resistance genes of Acinetobacter baumannii BL54, E.coli K12 J53.2
transconjugants and pseudomonas transformants in different soil microcosms.
Journal of Bacteriology Research 1(7): 79‐84 (2009).

9‐ MR. Shakibaie, Kyvan Shams, Mohammad Khalili. Detection of Legionella

pneumophila in Cooling Water Systems of Hospitals and Nursing Homes of Kerman
City, Iran by Semi‐Nested PCR. Int J Biol Life Sci. 5(1): 1‐4 (2009).

10‐ Shakibaie MR., Ardebili A., Aali S., Ketabchi A., Shahabinejad N. Antibiotic
resistance, β‐ lactamase production and plasmid profile of Neisseria gonorrhea
strains isolated from urethritis and cervicitis patients in Kerman, Iran. Eastern
Mediterranean health journal [EMRO] [WHO] to be published in (2009).

11‐ M R. Shakibaie, A. Khosravan, A. Frahmand, S. Zare. Application of metal resistant

bacteria by mutational enhancement technique for bioremediation of copper and
zinc from industrialWastes. Iran. J. Environ. Health. Sci. Eng. 5 (4): 251‐256 (2008).
(2 citations)

12‐ Abbas Mohammadi, Ali Vaziri Gohar and Mohammad Reza Shakibaie. Mutations
in Tumor Suppressor TP53 Gene in Formalin‐ Fixed, Paraffin Embedded Tissues of
Squamous Cell Carcinoma (SCC) of Lung Cancer. American Journal of Biochemistry
and Biotechnology. 4 (1): 1‐ 6 (2008).

13‐ MR. Shakibaie., F. Shahcheraghi., A. Hashemi., N. Saeed Adeli. Detection of SHV,

PER, and TEM type of ESBL genes among Pseudomonas aeruginosa isolated from
burn patients in Shafa ‐ hospital Kerman., Iran. IJBMS 11(2): 104‐111 (2008).

14‐ Shakibaie MR, Ardebili A, Alii SH, Ketabchi AA, Shahabinejad N. Antibiotic
Resistance, β‐Lactamase Production and Plasmid Profile of Neisseria gonorrhoeae
Strains Isolated from Urethritis and Cervicitis Patients in Kerman, Iran. Medical
Journal of Tabriz University of Medical Sciences. 30 (3): 13 (2008).

15‐ Mansouri S., Shakibaie M.R., Mahbob S. Isolation species distribution, antibacterial
resistance pattern and Beta‐lactamase production of enterococci isolated from
 human samples in southeast of Iran. I.J.M. S. 30(2): 68‐72 (2005).

16- Gholamhoseinian A., MR. Shakibaie and Z. Jamali. The mechanism of antibacterial
effect of methanolic extract of green Myrtus communis on E.coli K12 HB101. J
Rafsanjan Univ. Med. Sci. Health Serv. 4: 220-227 (2005).

17‐ Mohammad Reza Shakibaie and Ali Harati. Metal accumulation in Pseudomonas
aeruginosa Occur in the form of nanoparticles on the cell surface. Iranian Journal
of Biotechnology. 2(1):55‐60 (2004). (Book citation +1citation)

18‐ Shakibaie M.R., Dhakephalker B.A., Kapadnis B.P and Chopade B.A. Silver
resistance in Acinetobacter baumannii BL54 occurs through binding to a Ag‐Binding
Protein. Iranian Journal of Biotechnology. 1 (1): 41‐46 (2003). (18 citations)

19‐ Shakibaie M.R., Ehsan Z.H. Conjugative Plasmids among Antibiotic resistance
Escherichia Coli isolates from hospitals in Kerman, Iran. Modarres journal of
Medical Sciences. 6(2): 75‐83 (2003).

20‐ Shakibaie M.R. Plasmid mediated metal and antibiotic resistance in Pseudomonas
aeruginosa Strains isolated from burn patients. Medical Journal of the Islamic
Republic of Iran.16 (3):159‐ 163 (2002).

21‐ Kiani MA, Shakibaie MR. Kariminejad M.H. A 22;22 Robertsonian translocation in
patient with repeated abortions. Arch Irn Med; 3(3):151‐153, (2000).

22‐ Shakibaie M.R., Adeli S., Nikian Y. Emergence of Ciprofloxacin resistance among
Pseudomonas aeruginosa isolated from burn patients. Iranian Journal of Medical
Sciences. 26 (3&4): 155‐159, (2001).

23‐ Shakibaie M.R., Mansouri S., Hakak S. Plasmid Pattern of antibiotic resistance in
β‐lactamase producing Staphylococcus aureus isolated from hospitals in Kerman,
Iran. Arch Irn, Med.2 (2):93‐97 (1999). (4 citations)

24‐ Shakibaie MR, Dhakephalker P, Salajaghe G.A, Chopade BA. Plasmid mediated
silver & antibiotic resistance in Acinetobacter baumannii BL54. Iranian Journal of
medical sciences, 23 (1&2):30‐36 (1998). (21citations)
ABSTRACTS IN SEMINARS…………………………………………….
1‐ Shakibaie M.R. Antibiotic resistance among Pseudomonas aeruginosa exhibit different
RFLP with regards to gyrA gene. Proceedings of The second national biotechnology
Congress, Islamic Republic of Iran. Agricultural Biotechnology Research Institute of
Iran. Oct.9‐11, (2001), Karaj. (Invited speaker)

2‐ Shakibaie M.R: Gene therapy of monogenic diseases, new approach to genetics. 10th
Iranian Genetics Congress. 21‐23 May, (2008) Tehran, Iran. Pp‐401. (Invited speaker)

3‐ Shakibaie M R., Shams K., Khalili M: 16SRDNA PCR amplification and semi‐nested
PCR for detection of Legionella pneumophila in cooling water systems of different
hospitals and nursing homes of Kerman, Iran. 10th Iranian Genetics Congress. 21‐23
May, (2008) Tehran, Iran. Pp‐403.

4‐ Shakibaie M.R et al: Detection of ESBL genes and their heterogeneity in Pesudomonas
aeruginosa isolates. 3rd Conference of European Microbiologist (FEMS 2009)
Gothenburg, Sweden June 28‐July 2, 2009.pp1396

5‐ Shakibaie M.R et al: Horizontal transfer of antibiotic resistance genes among gram
negative bacteria in sewage and lake water and effect of some physicochemical of
water in conjugation. 3rd Conference of European Microbiologist (FEMS 2009)
Gothenburg, Sweden June 28‐July 2, 2009.pp139

6‐ Shakibaie MR; plasmid‐encoded resistance to penicillin’s in Acinetobacter baumannii BL54

mediated by carbenicillin‐hydrolysing (carb) type of β‐lactamase: 8th International
Symposium on the Biology of Acinetobacter1 – 3 September 2010 Università
di Roma Tre, Rome, Italy

Books Published………………………………………..

Basic Molecular Genetics. (1998).

Biotechnology and recombinants DNA technology.2003 (Translated

from English to Persian).

E‐book
Title Information
Title ID3403490
Title Principle of Basic Molecular Bacteriology
Volume Number1
Description: This book briefly describes the basic molecular bacteriology including
bacterial chromosome, molecular techniques used in bacteriology, quorum sensing,
Bacterial signal transduction, gene transfer among bacteria in the natural environment,
mitochondrial DNA, Index and References……...
ISBN1449542832
EAN‐139781449542832
Primary Category Science / General
Publication Date October 4 2009
Language English
Authored by Dr Mohammad Rza ‐ Shakibaie Ph.D.