AS Biology BYB1 Core Principles 10.

4 Enzymes Test
Name Date Question 1 2 3 4 5 6 7 8 9 Total % Grade
A = 36/45 (80%) B = 32/45 (70%) C = 27/45 (60%) D = 23/45 (50%) E = 18/25 (40%)

Marks /19 /5 /4 /3 /2 /4 /2 /4 /2 /45

Enzyme Properties 1. Complete the following paragraphs filling in the missing words (19 marks) energy of reactions. They are

Enzymes are biological catalysts, reducing the composed of

proteins with a specific tertiary structure.

In the complementary to the shape of the active site. In the active site alters to fit the substrate.

hypothesis, the substrate shape is hypothesis the

Increasing temperature increases the

energy of the enzyme and substrate .

molecules, with high temperature causing the enzyme to become irreversibly

Enzymes operate in a the

pH range, with changing pH around the enzyme disrupting .

Increasing enzyme concentration causes a

increase in the rate of an enzyme-

catalysed reaction, whereas increasing substrate concentration also increases rate but only until a is reached where all active sites are .

Some of the words in this box may be useful. Activation Globular Linear Narrow Shape of the substrate Denatured Indirect Lock and key Potential Substrate Enzyme Induced fit Maximum rate Saturated Unsaturated Fibrous Kinetic Minimum rate Shape of the active site Wide

2.

Draw a labelled sketch graph to show how temperature affects enzyme activity (5 marks)

3.

Explain in your own words, with a scientific reason, why this curve shape is produced (4 marks)

4.

Draw a labelled sketch graph to show how pH affects enzyme activity (3 marks)

5.

Explain in your own words, with a scientific reason, why this curve shape is produced. (2 marks)

6.

Draw a labelled sketch graph to show how substrate concentration affects enzyme activity. (4 marks)

7.

Explain in your own words, with a scientific reason, why this curve shape is produced. (2 marks)

8.

Draw a labelled sketch graph to show how enzyme concentration affects enzyme activity. (4 marks)

9.

Explain in your own words, with a scientific reason, why this curve shape is produced (2 marks)

(Maximum 45 marks) Mark scheme 1.) All words entered correctly (max 19) 2.) (max 5) 1 Enzyme activity 1 3.) Increased initial rate of reaction due to higher temperature causing more collisions (1) Plateau due to optimum temperature (1) 1 1 Fall due to excess temperatures causing enzymes to disintegrate and shape of active site lost (1) - becoming denatured (1) (max 4) 5.) Bell shaped curve - optimum pH in the centre (1) Lower at both sides due to active site shape changed due to charge (1) (max 2)

Temperature 4.) (max 3) 1 Enzyme activity 1

pH 6.) (max 4)

1 1 7.) Increased initial rate due to more substrate available to collide with enzymes (1) Plateau due to all active sites being filled (1) (max 2)

Enzyme activity 1

Substrate concentration

1 9.) Increased initial rate due to more enzymes available to collide with substrate (1) Plateau due to all active sites being filled (1) (max 2) (Max 45)

1 8.) (max 4) Enzyme activity 1 1

Enzyme concentration

A = 36/45 (80%) B = 32/45 (70%) C = 27/45 (60%) D = 23/45 (50%)

E = 18/25 (40%)