Lee Eun Joo

EXPRESSION AND FUNCTION OF MICRORNA IN HUMAN CANCER
DISSERTATION
Presented in Partial Fulfillment of the Requirements for the

Degree Doctor of Philosophy in the Graduate School of
The Ohio State University
By
Eun Joo Lee, M.S.
*****
The Ohio State University

2008
Dissertation Committee:
Dr. Thomas D. Schmittgen, Adviser
Approved by
Dr. James T. Dalton

Dr. Terry S. Elton
Dr. Mamuka Kvaratskhelia
Adviser
Graduate program in Pharmacy
Copyright by
Eun Joo Lee
2008
ABSTRACT
microRNAs (miRNA) are functional 22 nt noncoding RNAs that negatively

regulate gene expression. The mature miRNA binds to the 3 untranslated region of the
target messenger RNA (mRNA) and represses translation. There is mounting evidence
that miRNAs are involved in a number of human cancers. Pancreatic cancer is the fourth
leading cause of cancer-related death in the United States. The prognosis for pancreatic
cancer is the worst of all cancers. Better understanding of the genetic alterations in
pancreatic cancer may improve the early detection and treatment regimens.
The expression of over 200 miRNA precursors on specimens of human pancreatic
adenocarcinoma, paired benign tissue, normal pancreas, chronic pancreatitis and
pancreatic cancer cell lines was profiled using a real-time PCR assay developed in our lab.
The results showed that a number of miRNAs were differentially upregulated in the
tumors and cell lines compared to the normal pancreas tissue. The expression of the
active, mature miRNA was validated using a Taqman real-time PCR assay and Northern
blotting. Finally, RT in situ PCR was performed to verify that aberrantly expressed
miRNAs were localized to tumor cells and not to stroma or normal acini or duct cells.
To explore the mechanism of overexpressed miRNAs in pancreatic cancer and to
evaluate potential therapeutics, antisense oligonucleotides (ASO) were used to inhibit the
activity of the overexpressed miRNAs in pancreatic cancer. ASO produced cytotoxicity
ii
to pancreatic cancer cell lines in the low nM range. Cytotoxicity was less pronounced
when the normal ductal epithelial cell line HPNE was exposed to ASO or when
pancreatic cancer cell lines were exposed to an irrelevant control oligonucleotide.
Inhibition of miRNA with antisense increased the amount of apoptosis and resulted in
cell cycle arrest in HS766T cells compared to the control oligonucleotide. Synergistic
cytotoxicity was observed when ASO was combined with gemcitabine. The mRNA
targets of the aberrantly expressed miRNA in pancreatic cancer were validated and
identified to determine mechanism of action.
The miRNA processing pattern in different tissues and cell lines were determined
and a large number of miRNAs are transcribed but are not processed to the mature
miRNA.
iii
DEDICATION
To my parents
iv
ACKNOWLEDGMENTS
I would like to express sincere appreciation and gratitude to my advisor, Dr.

Thomas D. Schmittgen for his support and guidance. I have been very fortunate to have a
great teacher in the past five years who stimulated my interest in microRNA research.
Dr.Schmittgen guided my efforts to learn about microRNA and cancer and he always
encouraged me with his enthusiasm. I also would like to thank the members of my
dissertation committee, Dr. James T. Dalton, Dr. Elton S. Terry, and Dr. Mamuka
Kvaratskhelia for their comments and discussions.
I appreciate Yuriy Gusev for statistical analysis of my studies. I would like to
thank Daniel J. Brackett, Megan R. Lerner, Daniel L. Morgan, and Russell G. Postier for
providing human pancreatic tissues and cell lines and I would like to thank Dr. Wendy L.
Frankel for tissue specimen examination. I appreciate Dr. Caifu Chen for support of
Taqman real-time PCR assays and experimental discussions.
I am grateful to Dr.
Christine Esau for support of antisense oligonucleotides and expertise on antisense

studies. I also would like to thank to Dr. Gerard J. Nuovo for In situ RT PCR work.
I would like to thank Dr. Jinmai Jiang, Jong-Kook Park and Myungwon Baek for
their assistance with experiments, implementation, and data interpretation of the studies
performed herein. I wish to thank Ana Clara Azevedo and Liuqing Yang for their
friendship and help.
v
I would like to thank my parents, Sungho Lee and Keumsook Jeong, and my
brothers, Hyoshik Lee and Yongshik Lee, for their love, encouragement, and support.
I would like to thank Proctor and Gamble for the Fellowship. This research was
supported by a grant from NIH (CA107435-01 to T.D.S.) and the National Pancreas
Foundation.
vi
VITA
November 29, 1975... Born in Ulsan, South Korea

1995 1999B.S. Pharmacy, Seoul National University
Seoul, South Korea
1999 - 2001.M.S. Pharmacy, Seoul National University
Seoul, South Korea
2003 - Present.Graduate Teaching and Research Associate,
College of Pharmacy, The Ohio State University
PUBLICATIONS
1. Schmittgen, T.D., Lee, E.J., Jiang, J., Sarkar, A., Yang, L., Elton, T.S., Chen, C.
Real-time PCR quantification of precursor and mature microRNA. Methods,
44(1):31-38, 2008.
2. Lee, E.J., Baek, M., Gusev, Y., Brackett, D.J., Nuovo, G.J., Schmittgen, T.D.
Systematic evaluation of microRNA processing patterns in tissues, cell lines, and
tumors. RNA, 14(1):35-42, 2007.
3. Calin, G.A., Liu, C.G., Ferracin, M., Hyslop, T., Spizzo, R., Sevignani, C., Fabbri,
M., Cimmino, A., Lee, E.J., Wojcik, S.E., Shimizu, M., Tili, E., Rossi, S.,
Taccioli, C., Picchiori, F., Liu, X., Zuppo, S., Herlea, V., Gramantieri, L., Lanza,
G., Alder, H., Rassenti, L., Volinia, S., Schmittgen, T.D., Kipps, T.J., Negrini, M.,
Croce, C.M. Ultraconserved regions encoding ncRNAs are altered in human
leukemias and carcinomas. Cancer Cell, 12(3):215-229, 2007.
vii
4. Lee, E.J., Gusev, Y., Jiang, J., Nuovo, G.J., Lerner, M.R., Frankel, W.L., Morgan,
D.L., Postier, R.G., Brackett, D.J., Schmittgen, T.D. Expression profiling
identifies microRNA signature in pancreatic cancer. International Journal of
Cancer, 120(5):1046-1054, 2007.
5. Lee, E.J. and Schmittgen, T.D. Comparison of RNA assay methods used to
normalize cDNA for quantitative real-time PCR. Analytical Biochemistry,
357(2):299-301, 2006.
6. Martin, M.M., Lee, E.J., Jiang, J., Buckenberger, J., Schmittgen, T.D., and Elton,
T.S. microRNA-155 regulates human angiotensin II type 1 receptor expression in
fibroblasts. Journal of Biological Chemistry, 281(27):18277-18284, 2006.
7. Jiang, J., Lee, E.J. and Schmittgen, T.D. Increased expression of microRNA-155
in Epstein-Barr Virus transformed lymphoblastoid cell lines.
Genes,
Chromosomes & Cancer, 45(1):103-106, 2006.
8. Jiang, J., Lee, E.J., Gusev, Y and Schmittgen, T.D. Real-time expression
profiling of microRNA precursors in human cancer cell lines. Nucleic Acids
Research, 33(17):5394-5403, 2005.
9. Lee, E.J., Kim, E.J., Kim, Y.G., Chung, H.C., Kim, S.H., Kim, D.H., Lee, I., Kim,
S.G., Lee, M.G. Pharmacokinetic changes of intravenous 2-(allylthio) pyrazine, a
chemoprotective agent, in rats with acute renal failure induced by uranyl nitrate.
Research Communications in Molecular Pathology and Pharmacology, 111(56):225-236, 2002.
10. Shim, H.J., Lee, E.J., Jung, Y.H., Kim, S.H., Kim, S.H., Yoo, M., Kwon, J.W.,
Kim, W.B., Lee, M.G. Determination of a new phosphodiesterase V inhibitor,
DA-8159, in plasma and urine by high-performance liquid chromatography.
Journal of Pharmaceutical and Biomedical Analysis, 30(3):527-533, 2002.
11. Shim, H.J., Lee, E.J., Kim, J.H., Kim, S.H., Kwon, J.W., Kim, W.B., Cha, S.W.,
Lee, M.G. Subacute toxicities and toxicokinetics of a new erectogenic, DA-8159,
after single and 4-week repeated oral administration in dogs. Biopharmaceutics &
Drug Disposition, 22(3):109-117, 2001.
12. Shim, H.J., Lee, E.J., Kim, S.H., Kim, S.H., Yoo, M., Kwon, J.W., Kim, W.B.,
Lee, M.G. Factors influencing the protein binding of a new phosphodiesterase V
viii
inhibitor, DA-8159, using an equilibrium dialysis technique. Biopharmaceutics &

Drug Disposition, 21(7):285-291, 2000.
13. Shim, H.J., Lee, E.J., Kim, S.H., Kim, S.H., Yoo, M., Kwon, J.W., Kim, W.B.,
Lee, H.S., Lee, M.G. Pharmacokinetics, stability, and blood partition of DA-8159,
a new phosphodiesterase V inhibitor. Research Communications in Molecular
Pathology and Pharmacology, 108(3-4):275-286, 2000.
14. Hurh, E., Lee, E., Lee, A., Kim, Y., Kim, S., Kim, S., Lee, M. Effects of enzyme
inducers or inhibitors on the pharmacokinetics of intravenous parathion in rats.
Biopharmaceutics & Drug Disposition, 21(5):193-204, 2000.
15. Hurh, E, Lee, E.J., Kim, Y.G., Kim, S.Y., Kim, S.H., Kim, Y.C., Lee, M.G.
Effects of physostigmine on the pharmacokinetics of intravenous parathion in rats.
Biopharmaceutics & Drug Disposition, 21(8):331-338, 2000.
16. Hurh E, Lee, E.J., Kim, Y.G., Kim, S.Y., Kim, S.H., Kim, Y.C., Lee, M.G.
Effects of neostigmine on the pharmacokinetics of intravenous parathion in rats.
Research Communications in Molecular Pathology and Pharmacology, 108(34):261-273, 2000.
FIELDS OF STUDY
Major Field: Pharmacy
ix
TABLE OF CONTENTS
ABSTRACT ......................................................................................................................ii
DEDICATION .................................................................................................................iv
ACKNOWLEDGMENTS ................................................................................................v
VITA ................................................................................................................................vii
LIST OF TABLES .........................................................................................................xvi
LIST OF FIGURES ......................................................................................................xvii
CHAPTERS
1. INTRODUCTION ................................................................................................1
1.1 miRNA DISCOVERY ......................................................................................1
1.2 miRNA BIOGENESIS .....................................................................................3
1.3. miRNA TARGET IDENTIFICATION ...........................................................6
1.4 miRNA EXPRESSION AND PROFILING .....................................................8
1.5 miRNA EXPRESSION IN HUMAN CANCER ............................................10
1.6 miRNA THERAPEUTICS .............................................................................14
1.6.1 Inhibition of miRNA using antisense oligonucleotides (ASO) .......15
1.6.2 Replacement of miRNA ...................................................................16
1.7 OBJECTIVE OF DISSERTATION ...............................................................17
x
2. EXPRESSION PROFILING IDENTIFIES MICRORNA SIGNATURE IN

PANCREATIC CANCER ..................................................................................21
2.1 INTRODUCTION ..........................................................................................21
2.2 MATERIAL AND METHODS ......................................................................22
2.2.1 Tissue procurement ..........................................................................22
2.2.2 Cell lines ..........................................................................................23
2.2.3 miRNA precursor expression profiling ............................................23
2.2.4 Real-time PCR of mature miRNA ...................................................24
2.2.5 Northern blotting ..............................................................................25
2.2.6 RT in situ PCR .................................................................................25
2.2.7 Statistics ...........................................................................................26
2.3 RESULTS .......................................................................................................28
2.3.1 Validation .........................................................................................28
2.3.2 miRNA precursor profiling in pancreatic tissues .............................28
2.3.3 Data analysis by prediction analysis of micro-array algorithm (PAM)
..........................................................................................................30
2.3.4 Validation of miRNA expression .....................................................31
2.4 DISCUSSION .................................................................................................33
2.5 ACKNOWLEDGEMENTS ............................................................................37
xi
3. ANTISENSE INHIBITION OF microRNA REDUCES CELL VIABILITY

AND SENSITIZES THE EFFECT OF GEMCITABINE IN PANCREATIC
CANCER .............................................................................................................48
3.1 INTRODUCTION ..........................................................................................48
3.2.1 Cell lines and culture .......................................................................52
3.2.2 miRNA profiling ..............................................................................52
3.2.3 Antisense oligonucleotides ..............................................................53
3.2.4 Antisense oligonucleotides transfection ..........................................53
3.2.5 Gemcitabine treatment .....................................................................53
3.2.6 Cell proliferation assay ....................................................................54
3.2.7 Combination assay ...........................................................................54
3.2.8 Data analysis ....................................................................................55
3.2.9 Statistical analysis ............................................................................56
3.3 RESULTS .......................................................................................................56
3.3.1 miRNA expression in pancreatic cell lines ......................................56
3.3.2 Antiproliferative effects of antisense oligonucleotides ....................57
3.3.3 Schedule-dependent combinatorial effects of antisense with
gemcitabine ......................................................................................59
3.3.4 Evaluation of synergism in ASO combination ................................60
xii
3.4 DISCUSSION .................................................................................................62

4. ANTISENSE INHIBITION OF microRNA ARRESTS CELL CYCLE,
INDUCES APOPTOSIS AND IDENTIFIES THE TARGET MOLECULES
IN PANCREATIC CANCER ............................................................................77
4.1 INTRODUCTION ..........................................................................................77
4.2.1 Cell lines and culture .......................................................................80
4.2.2 Antisense Oligonucleotides and Pre-miR miRNA Precursor ......80
4.2.3 Antisense oligonucleotide and Pre-miR miRNA Precursor
transfection ......................................................................................80
4.2.4 Cell cycle analysis ............................................................................80
4.2.5 Apoptosis analysis ...........................................................................81
4.2.6 Real-time PCR .................................................................................81
4.2.7 Plasmid constructs ...........................................................................82
4.2.8 Luciferase assay ...............................................................................82
4.2.9 Western blotting ...............................................................................83
4.3. RESULTS ......................................................................................................83
4.3.1 Effects of miRNA inhibition on the cell cycle .................................83
4.3.2 Effect of miRNA inhibition on apoptosis ........................................84
xiii
4.3.3 PTEN, RECK and p27(kip1) validation as target of miR-21 and miR221 ....................................................................................................85
4.3.4 FOXO3a as a new target of miR-212 ..............................................86
4.4 DISCUSSION .................................................................................................88
5. SYSTEMIC EVALUATION OF microRNA PROCESSING PATTERNS IN
TISSUES, CELL LINES AND TUMORS ........................................................97
5.1 INTRODUCTION ..........................................................................................97
5.2.1 Cells, tissues and RNA ....................................................................99
5.2.2 Real-time RT PCR, miRNA precursors, and mature miRNA .........99
5.2.3 Real-time RT PCR, absolute copy number for precursor and mature
miRNA .........................................................................................100
5.2.4 Northern blotting ............................................................................101
5.2.5 In situ detection of miRNA ............................................................101
5.2.6 Data analysis ..................................................................................101
5.3 RESULTS .....................................................................................................102
5.4 DISCUSSION ...............................................................................................107
5.5 ACKNOWLEDGMENTS ............................................................................109
6. SUMMARY AND DISCUSSION ....................................................................117
xiv
BIBLIOGRAPHY..........................................................................................................124
APPENDICES
APPENDIX A: Data Relevant to Chapter 2 .......................................................147
Table A ....................................................................................................148
Figure A ..................................................................................................198
APPENDIX B: Data Relevant to Chapter 3 .......................................................199
Table B ....................................................................................................200
APPENDIX C: Data Relevant to Chapter 5 .......................................................215
Table C.1..................................................................................................216
Table C.2..................................................................................................251
Table C.3..................................................................................................287
Table C.4..................................................................................................302
Table C.5..................................................................................................316
Table C.6..................................................................................................321
xv
LIST OF TABLES
Page
Table
2.1
Clinical data and tumor pathology ........................................................................46
2.2
Top 20 aberrantly expressed miRNA precursors in pancreatic adenocarcinoma .47
3.1
Sequence and chemical modifications to antisense oligonucleotides ...................73
3.2
miRNA expression, pancreas cancer cell lines normalized to normal pancreas cell
(HPNE) .................................................................................................................74
3.3
Antiproliferative activity parameters of ASO against miR-21, -100, -212, -221

and -301 in human pancreatic cell lines ................................................................76
5.1
microRNA that display tissue-specific or tissue enriched expression ................115
5.2
Relative expression levels of mature miRNA and miRNA forms ....................116
xvi
LIST OF FIGURES
Page
Figure
1.1
miRNA biogenesis ................................................................................................19
1.2
Chemical modification of antisense oligonucleotides ..........................................20
2.1
18S rRNA expression in pancreatic tissue ............................................................38
2.2
miRNA precursor expression in pancreatic samples ............................................39
2.3
Three dimensional expression terrain map ...........................................................40
2.4
Estimated probabilities for the training and test data ............................................41
2.5
miRNA expression by Northern blotting ..............................................................42
2.6
Validation of precursor and mature miRNA levels ..............................................44
2.7
Histologic and molecular analysis of pancreatic cancer for microRNA

expression .............................................................................................................45
3.1
Structures of gemcitabine and deoxycytidine .......................................................65
3.2
Comparison of three antisense oligonucleotides ...................................................66
3.3
The effect of antisense oligonucleotide and control oligonucleotide in pancreatic

cell lines ................................................................................................................68
3.4
Effect of control oligonucleotides on cell proliferation ........................................69
3.5
ASO pretreatment sensitized the antiproliferative effects of gemcitabine ...........71
3.6
Combination index (CIx) versus fraction affected (fa) plots in HS766T cells .....72
xvii
4.1
Flow cytometric analysis of the effect of antisense oligonucleotide (ASO) on cell

cycle of HS766T cells ...........................................................................................92
4.2
Flow cytometric analysis of apoptosis in HS766T cells .......................................93
4.3
Western blot analysis of PTEN, RECK and p27(kip1) in HS766T cells treated
with antisense to miR-21 or miR-221 ...................................................................94
4.4
Alignment of the FOXO3a binding site with miR-212 and mRNA expression of
FOXO3a after ASO treatment .............................................................................95
4.5
Target validation by luciferase assay and FOXO3a western blotting ...................96
5.1
microRNAs with good correlation in expression levels of precursor and

mature .................................................................................................................110
5.2
Expression of miR-128a in 22 normal tissues and cells .....................................111
5.3
Lack of microRNA processing in cancer cell lines ............................................112
5.4
miR-31 expression in cancer cell lines ...............................................................113
5.5
Pearson correlation among the various samples studied .....................................114
6.1
Pancreatic cancer progression model and genetic alteration .............................123
xviii
CHAPTER 1
INTRODUCTION
1.1 miRNA DISCOVERY
Until very recently, the primary role of RNA was believed to carry genetic
information for protein translation. The discovery of small non-coding RNAs and other
noncoding RNAs has caused many to rethink the role of RNA in biology. microRNA
(miRNA) is endogenous ~ 22 nt non-coding RNA that negatively regulates gene
expression (1-3). miRNA was discovered by the Ambros and Ruvkun laboratories during
genetic screening of developmental progress in C. elegans (4, 5).
The lin-4 gene
produced ~ 22 nt noncoding RNA that binds to sequences in the 3 untranslated region

(3 UTR) of lin-14 mRNA (4).
The interaction between lin-4 and lin-14 caused
translational repression of the lin-14 mRNA (4). The Ruvkun laboratory also discovered
that another small non-coding RNA, let-7, recognized the 3 UTR of lin-41 mRNA and
repressed LIN-41 protein levels (6). let-7 was conserved in a wide range of animals,
suggesting that other ~ 22 nt regulatory RNAs may exist in other organisms (7).
Shortly thereafter, RNA interference (RNAi) was uncovered in C. elegans by the
Fire and Mello laboratory.
They discovered that double-stranded RNA (dsRNA)

1
produced target gene silencing, in which the target mRNA is cleaved in a sequencespecific manner (8). Twenty-two to twenty-five nt duplex RNA, named small interfering
RNAs (siRNAs), was discovered by the Tuschl laboratory (9).
siRNAs mediate
sequence-specific mRNA degradation during RNA interefence (RNAi).
siRNA is
generated from long dsRNA by the RNase III, Dicer and it is incorporated into the RNA
induced silencing complex (RISC). mRNA that is 100% complementary to the siRNA
(10) is cleaved. RNAi has been a powerful tool of gene silencing and has been applied in
a wide range of human diseases.
In 2001, interest in the field peaked when hundreds of miRNAs were discovered
in Drosophila, C. elegans and mammals (1-3). These ~ 22 nt endogenous RNAs, derived
from longer hairpin precursors, were named microRNAs (miRNAs).
Using
bioinformatics and cDNA cloning methods, miRNA sequences were uncovered based
upon the following criteria; expression of ~ 22 nt mature RNA in one arm of the hairpin
structure, location in intergenic regions, DNA sequence homology between similar
species and containing a stem-loop structure in the precursor transcript (1-3).
The
expression of miRNAs was also examined during larval stages and young adults in C.
elegans and gene expression was varied in different stages of development,
demonstrating that they play an important role in developmental timing (1-3). A number
of miRNAs have been identified in a wide range of species such as plant, nematodes,
fruit flies, and human and more miRNA genes are expected to be discovered in many
species (11). At the time of writing, 678 Homo sapiens miRNAs have been discovered
(12).
1.2 miRNA BIOGENESIS
Human miRNAs are distributed in all chromosomes except the Y chromosome.

Initially, miRNAs were thought to be located only within intergenic regions (1-3).
However, Rodriguez et al. reported that around 70% of mammalian miRNAs are located
in defined transcription units (TU) i.e. intronic and exonic miRNAs (13). Among those
miRNAs, 61% RNAs are located in the introns of protein-coding TU, 18% are located in
the introns of non-coding TU and some are found in exons of non-coding gene (20%)
(13).
Most miRNAs are transcribed by RNA polymerase II.
However, miRNAs
positioned within Alu-repetitive elements are transcribed by RNA polymerase III (14).
Some miRNAs are clustered and transcribed together as long polycistronic RNAs.
Clustered miRNAs seem to be functionally linked to each other (15). miR-23a, -27a, 24-2 and miR-17, -18, -19a, -20, -19b-1 are examples of polycistronic miRNA transcripts
(16). An overall picture of miRNA biogenesis is shown in Figure 1.1.
The primary transcript, called the pri-miRNA contains a 5 7-methyl guanosine
cap and 3 polyadenylate tail (17, 18). Pri-miRNA is processed in the nucleus by Drosha,
an RNase III endonuclease to produce 60 to 70 nt pre-miRNA; the flanking sequences
around the hairpin structure are believed to be degraded (19). The ~ 160 kDa Drosha
crops the stem of the pri-miRNA hairpin structure asymmetrically, i.e. it produces a 2 nt
3 overhang on the pre-miRNAs. Drosha forms a microprocessor complex of ~ 650 kDa
with the DiGeorge syndrome critical region gene 8 (DGCR8); this complex is conserved
from flies to human (20-23). Drosha shows non-specific RNase activity, but DGCR8
contains two double-stranded RNA binding sites at the C-terminus. This helps Drosha
3
correctly recognize and cleave the hairpin structure at the specific site (20-23). DGCR8
also has a WW domain at the N-terminus, which is believed to interact with proline-rich
peptides and in the proline-rich N-terminus of Drosha (20). Neither Drosha nor DGCR8
shows any processing activity independently (22).
The pre-miRNAs are then exported to the cytoplasm by Exportin-5; a Randependent nuclear transport receptor (24). Exportin-5 especially transports the minihelix
motif RNAs, which contain a double-stranded stem (> 14 nt) with 3' overhang on the end
(25). ILF3, which is a nuclear protein containing two double-stranded RNA binding
domain and RGG domain forms a quaternary complex with the minihelix motif RNA and
Exportin-5, indicating that ILF3 facilitates transport of pre-miRNA along with Exportin-5
(26). It was believed that if Exportin-5 is inhibited by siRNA, the pre-miRNA would
accumulate and mature miRNA would decrease (24). However, pre-miRNA did not
accumulate in the nucleus, which suggests that pre-miRNA is a quite unstable
intermediate in the absence of Exportin-5 (24).
In the cytoplasm, pre-miRNAs are processed into ~ 22 nt mature miRNA by
another RNase III endonuclease, Dicer (27). Dicer is a highly conserved protein. Dicer
binds with other cofactors such as Loquacious/HIV-1 TAR RNA binding protein (TRBP)
(28, 29). Dicer cleaves the loop of the hairpin to produce the miRNA:miRNA duplex.
The duplex contains a 5 phosphate and 2 nt 3 overhang.
Dicer-TRBP recruits
Argonaute protein (Ago 2) to form the RNA-induced silencing complex (RISC)

incorporating the mature miRNAs into the RISC (30). One strand of the duplex miRNA
is degraded and the other strand remains as the mature miRNA. The decision by RISC
as to which strand to degrade is based on base pair stability at the 5 ends of the duplex
4
miRNA; the miRNA strand with relatively lower stability at 5 end will remain in RISC
(31).
Finally, the mature miRNA that is incorporated into RISC binds to the 3 UTR of
the target mRNA and mediates translational repression or target cleavage according to
sequence complementary between miRNA and target mRNA (31). If the mRNA and
miRNA have perfect complementary, miRNA will cleave the mRNA much like a siRNA.
In animals, it is more likely that the miRNA/mRNA interaction will not have perfect
complementarity and the miRNA will repress translation (32). mRNA destabilization is
caused by deadenylation and decapping (33). Translational initiation or elongation might
also be inhibited (34, 35). However, the Steitz laboratory recently reported that miRNA
may also up-regulate translation during cell cycle arrest, indicating that translational
regulation by miRNA can be either repressive or activating (36). miRNA is generally
thought to bind to the sequence of 3 UTR. However, recent reports showed that miRNA
can also bind to other sites. For example, let-7 binds to the mRNA target sites in either
the coding region or the 5 UTR in zebrafish (37) and the Steitz laboratory demonstrated
that miRNA binds the target mRNA efficiently in the 5 UTR as in the 3 UTR (38).
Also, the Lund laboratory showed that miR-10a binds to the 5 UTR of ribosomal
proteins mRNA and enhances the translation, suggesting that miR-10a may control global
protein synthesis (39). These studies imply that miRNA has more diverse and complex
functions.
Recent work has demonstrated that miRNA biogenesis may be regulated in
developmental stages or specific tissues (40-44).
One control mechanism is
transcriptional regulation. let-7 is regulated by a transcriptional enhancer element in C.

5
elegans (41).
Another mechanism is post-transcriptional regulation.
pri-miR-18a
requires an RNA-binding protein hnRNP A1 for processing to the precursor (40). The
RNA binding protein, lin-28 inhibits the processing of pri-let-7 miRNAs in embryonic
cells (44). pre-miR-138-2 is restricted to Dicer cleavage in certain tissues and cell types
(43). Finally RNA editing, which converts adenosine (A) to inosine (I), can inhibit Dicer
cleavage. Edited pre-miRNA accumulates, indicating that Dicer-TRBP complex cannot
bind to edited pre-miRNA (42).
1.3 miRNA TARGET IDENTIFICATION
miRNAs control gene expression by binding to the 3 UTR of target mRNA. It is

estimated that approximately 30% of human genes are regulated by miRNA (45).
Although 678 human miRNAs have been reported, identification and validation of their
targets has been a challenge. Knowing the miRNAs target is crucial to identifying the
miRNAs function.
The first miRNA target was the lin-14 gene (4).
Further studies (46, 47)
identified the criteria for the miRNA/mRNA interaction by mutating known target
binding sites. Of particular importance is the 5 seed region of the miRNA. This 5 seed,
consisting of nts 2-8, must bind perfectly to the target mRNA sequence. The Cohen
laboratory reported the minimal target site for miRNA function (46). They mentioned
that seven or more sequences that are complementary at the 5 end of the miRNA are
enough to show function.
If the 5 sequence has weaker binding, then the 3
compensatory binding is required. However, extensive binding at the 3 site without 5

6
complementarity is not enough to have miRNA function (46).
Even though 5
complementary sequence is important to predict the target gene, 3 complementary site is

also important to distinguish the target of miRNA isoforms. miRNA isoforms may target
the same gene or different genes. For example, let-7 family members (e.g. let-7, miR-48,
miR-84 and miR-241) have weak seed binding to one known target (lin-41), but only let7 has strong 3 binding and lin-41 is regulated by only let-7 among let-7 family members.
In addition, G:U wobble in the seed sequence also showed negative effect in binding
activity (46).
Other elements may affect target binding. Secondary structure in 3 UTR of the
mRNA may prevent the miRNA from binding to the site (48). Several laboratories imply
that target regulation by miRNA is also dependent on 3 UTR context. For example, the
function of CAT-1, which is a target of miR-122, depends on the presence of HuR
protein binding site (49) and lsy-6 functionally binds to the target site (cog-1) in
endogenous 3 UTR, but not in a heterologous 3 UTR (50).
Several target prediction programs have been developed. The main algorithms
used in these programs include a 5 seed sequence match and a calculation for the
thermodynamic
stability
between
miRNA
and
mRNA.
TargetScan
(http://www.targetscan.org) (45, 47, 51) is one program used to predict mammalian

miRNA targets. It finds conserved 8 mer and 7 mer sites that match the seed sequence of
the miRNA and calculates the free energy using the RNA Fold algorithm. A false
positive rate of between 22-31% may be expected from TargetScan.
PicTar
(http://pictar.bio.nyu.edu) (52-54) is used to identify miRNA target genes in C. elegans,

Drosophila, and vertebrates. It identifies targets for single miRNA as well as targets that
7
are regulated by several miRNAs in a coordinated manner. It predicts around 200 targets
per miRNA with an estimated false positive rate of around 30%. Lastly, miRanda
(http://microrna.sanger.ac.uk) (55) uses sequence match and binding energy between the
miRNA and the predicted target site. Evolutionary conservation of the target binding site
is applied; miRanda has a 24-39% false positive rate.
However, prediction programs dont account for all of the criteria. For example,
3 UTR accessibility and/or 3 UTR context (specific RNA- or protein-based cofactors)
as well as the 5 seed region and thermodynamics may be important criteria for target
prediction. Combining all these possible factors can produce better performance to
predict the miRNA target. Moreover, a single miRNA targets numerous genes and one
target gene is regulated by multiple miRNAs. This suggests that miRNA and target
network might be more complicated than originally expected and that experimental
validation should be performed carefully.
1.4 miRNA EXPRESSION AND PROFILING
miRNA profiling is a major discovery tool to identify tissue-specific or diseasespecific miRNAs. Several methods are available to profile miRNA expression. These
include Northern blotting, microarray, real-time PCR and in situ hybridization. The
benefit of Northern blotting is that both mature and precursor transcripts are visualized at
the same time on the blot. However, it is a laborious technique with poor sensitivity and
requires large amounts of RNA (10-30 g). Another method is cDNA microarray (56).
cDNA arrays are used in many laboratories for high-throughput screening since
8
thousands of genes may be placed on the array. cDNA microarray is based on the
hybridization between the target gene and probe. However, it often shows low sensitivity
and variation in signal intensity (57). In addition, microarray data should be corrected
from background and normalized to reduce dye labeling bias, variations in hybridization
and scanning (58). Therefore, the microarray data should be confirmed by northern blot
or real-time PCR. Real-time PCR is the most sensitive of the high-throughput screening
methods. Performing real-time PCR on miRNA is a challenge because the precursors
exist as a stable hairpin and the mature miRNA is ~ 22 nt, roughly the size of a standard
PCR primer. The Schmittgen laboratory developed a PCR assay to amplify precursor
miRNA as a means to predict the mature miRNA expression (59). Shortly thereafter,
Applied Biosystems developed a real-time PCR assay to the the mature miRNA (60).
This assay is named the looped primer PCR assay because the RT primer consists of a
stable loop structure. Stem-loop RT primers were developed for several reasons. It
increases the annealing temperature to the mature miRNA. It also avoids precursor
miRNA or genomic DNA from being amplified. Finally, it extends the size of the ~ 22 nt
mature miRNA. Since the mature assay uses TaqMan probes, it enhances sensitivity to
distinguish miRNA family members (61). The assay is sensitive enough to profile the
miRNA expression from a single cell (62, 63).
miRNA profiling of human cancers is a useful tool to classify cancer type
according to their origin. Jiang et al. (61) profiled around 200 miRNA precursors in 32
human cancer cell lines and hierarchical clustering showed that most of cell lines are
clustered into each tissue type based on their origin. Also, human pancreatic tumor
tissues and normal tissues were used to profile miRNA precursors and the heatmap
9
results demonstrated that the tumor tissue group was completely separated from the
normal tissue group (64). Golubs group (65) profiled miRNAs in 334 human samples
using a bead-based flow cytometric method. Many miRNAs were down-regulated in the
tumor samples.
Hierarchical clustering classified the epithelial and hematopoietic
samples based on the developmental origins of the tissues. Interestingly, cancers within a
single cell lineage were further discriminated according to their genetic abnormality.
Profiling data from 200 miRNAs correctly classify each tumor group, but profiling data
from around 16,000 mRNAs did not (65). This evidence suggests that miRNA profiling
can distinguish the phenotypic signature of diverse types of cancers and that miRNA may
be used as a potential diagnostic marker.
1.5 miRNA EXPRESSION IN HUMAN CANCER
Several miRNAs are tissue-specific or developmental specific, indicating that

they could play important roles in many biological processes. For example, miRNA-143
regulates adipocyte differentiation (66), miRNA-375 regulates insulin secretion (67),
human embryonic stem cells express a unique set of miRNAs (68), and miRNA also
controls oocyte maturation in Drosophila (69).
miRNA expression also plays a role in the initiation and progression of certain
diseases. miR-1 was identified to play a key role in cardiomyocyte differentiation and
cardiovascular development (70). Human angiotensin II type 1 receptor, which function
as vasoconstrictor and plays a role in the pathogenesis of cardiovascular diseases, is
regulated by miR-155 (71). miRNA is also linked to several neurodegenerative diseases
10
such as X mental retardation syndrome (72), Alzheimers disease (73) and Parkinsons
disease (74).
In addition, there is mounting evidence that miRNAs are involved in a number of
human cancers and that miRNAs may act as both tumor suppressors and oncogenes. A
decrease in the expression of a tumor suppressive miRNA will cause an increase in the
target oncogene. In contrast, an increase in expression of oncogenic miRNAs will lead to
the reduction of the target tumor suppressor. Some miRNAs are overexpressed in cancer
and some are down-regulated in cancer. For example, miR-15, -16 (75), miR-143, -145
(76-78), let-7 (79), miR-199a, -199a (80-82) and 122a (81, 83) are reduced in cancer.
miR-21 (84-87), miR-221, -222 (88-90), miR-100 (88) and miR-155 (91) are increased in
cancer. It is still uncertain if differentiated miRNA expression is a cause or outcome of
tumor transformation. Kumar et al. (92) knocked down Drosha and Dicer enzymes in
cell lines producing a global reduction of mature miRNA expression. This reduction in
mature miRNA levels caused an increase in cell growth. Using a Dicer deficient lung
cancer mouse model, tumor burden (area, size and number) was increased compared to
control. Furthermore, RAS and c-Myc were up-regulated, suggesting that a global loss of
miRNA boosts tumorigenesis.
Each cancer type shows a specific miRNA expression.
Perhaps the best
characterized miRNAs in human cancer is miR-15 and miR-16 in human chronic

lymphocytic leukemia (CLL). Deletion of the chromosome 13q14 region is common in
more than 50% of CLL; miR-15 and miR-16 are located at 13q14.3 (75). The Croce
laboratory showed that miR-15 and miR-16 were deleted or down-regulated in more than
60% of CLL samples and that these miRNAs negatively regulate BCL-2 (93). Another
11
example is the miR-17-92 cluster. This cluster is increased in human B-cell lymphomas
and is located at 13q31.3, which is amplified in B-cell lymphoma (94). He et al. also
showed that expressing the miR-17-92 clutster in mice accelerated c-Myc induced tumor
formation, demonstrating that miR-17-92 cluster is oncogenic.
In contrast, c-Myc
negatively regulates the transcription of the miR-17-92 cluster and two miRNAs from
this cluster (miR-17-5p and miR-20a) negatively regulate the transcription factor E2F1
(95), suggesting this cluster functions as a tumor suppressor miRNA. Theses results
suggest that the identical miRNA may show both functions depending on tissue of cell
types. miR-155/BIC RNA is elevated in B-cell lymphomas (91). The expression of miR155 was higher in cells with an activated B cell phenotype compared to the germinal
center phenotype. Since patients with activated B cell type lymphoma show worse
prognoses, this suggests that miR-155 may be used for as a diagnostic marker (91). The
Croce laboratory showed that a miR-155 overexpressing transgenic mouse displayed preleukemic pre-B cell proliferation in spleen and bone marrow, followed by B cell
malignancy (96).
The expression of mature miR-143 and miR-145 were decreased in colorectal
neoplasia (76). Interestingly, the expression of the hairpin precursors in normal and
tumor tissues remained constant, demonstrating posttranscriptional processing is reduced
in cancer. In lung cancer, let-7 is down-regulated in the tumor and patients with reduced
let-7 had a reduced postoperative survival (79). RAS oncogene is a possible target gene
of let-7 (97) and Yanaihara et al. reported that high expression of miR-155 and low
expression of let-7a-2 correlated with poor survival in lung cancer (98). Several miRNAs
12
are also involved in human breast cancer (99), papillary thyroid carcinoma (90), primary
glioblastoma (100), and hepatocellular carcinoma (82).
Some miRNAs are implicated in several types of cancer. In particular, miR-21 is
overexpressed in B cell lymphoma (101), CLL (102) and many solid tumors, e.g.
pancreatic cancer (64), ovarian cancer (103), esophageal cancer (104), uterine
leiomyomas (105), head and neck cancer (106), colorectal cancer (107) gastric cancer
(108). It was shown that miR-21 is up-regulated in glioblastoma and knockdown of miR21 enhanced apoptosis, suggesting miR-21 targets apoptosis-related genes (87). Iorio et
al. (99) and Si et al. (85) also found that miR-21 is overexpressed in breast cancer. Si et
al. showed that anti-miR-21 oligonucleotide decreased cell growth in vitro and in vivo
and they suggested that miR-21 is an oncogene that is associated with the anti-apoptotic
gene BCL-2. Zhu et al. (109) used two-dimensional gel electrophoresis to identify
targets of miR-21 and found that the tumor suppressor gene tropomyosin 1 (TPM1) as a
target of miR-21 in a breast cancer cell line. The Patel laboratory demonstrated that miR21 is highly expressed in hepatocellular cancer (86, 110). Inhibition of miR-21 sensitized
the effect of gemcitabine and miR-21 functioned in cell proliferation, migration and
invasion targeting the phosphatase and tensin homolog of PI3 kinase signaling (PTEN).
Recently, a paper showed that the tumor suppressor gene, programmed cell death 4
(PDCD4), is also negatively regulated by miR-21 and that oncogenic miR-21 promotes
cell transformation (111). Lffler et al. explained that miR-21 contributes to signal
transducer and activator of transcription 3 (Stat3)-mediated survival of myeloma cells
(112).
13
This body of evidence indicates that miRNA may act as oncogenes or tumor
suppressor genes and that miRNAs are involved in cell proliferation, apoptosis, cell cycle,
development and metabolism. Identification of miRNAs and the genes they regulate may
provide potential targets for diagnosis and therapy in human cancer.
An important question is what are the causes of differential expression of miRNA
in cancer? Because cancer is a complex genetic disease, the cause might be complicated.
Many miRNAs are located in genomic regions that are involved in cancer including
minimal regions of loss of heterozygosity (LOH), minimal regions of amplification, or
common breakpoint regions (113). Zhang et al. showed that alterations in the DNA copy
of genes that are involved in miRNA biogenesis, such as Dicer 1 and Argonaute 2 may
regulate miRNA expression in tumors (114). In addition, epigenetic regulation such as
DNA hypomethylation, CpG island hypermethylation and the alterations of histone
acetylation (115), transcriptional, posttranscriptional, and translational regulation may
explain the deregulation of miRNA expression in tumors. For example, miR-124a and
miR-127 are inactivated by hypermethylation in several human cancers (116, 117).
1.6 miRNA THERAPEUTICS
As mentioned above, one third of the human genome is regulated by miRNA.

Since one miRNA targets multiple mRNAs, one deregulated miRNA in cancer can affect
the expression of scores of downstream genes. The expression of miRNA is deregulated
in many types of cancer, indicating that miRNAs play important roles in cancer.
Therefore, cancer-related miRNAs are promising targets for therapeutics.
14
The most
common
method
of inhibiting miRNA is
with
antisense
oligonucleotides (ASO). Other possible options for miRNA blocking are using target
protector or targeting miRNA processing molecules.
Target protector binds to the
miRNA binding site in the 3 UTR of mRNA and it inhibits the miRNAs access to the
binding site (118). Target protector has the complementary sequence of the seed region
and additional flanking sequence of the 5 or 3 binding site. Many molecules, including
Drosha and Dicer are involved in miRNA processing and inhibiting the processing steps
in cancer with increased miRNA expression could reduce mature miRNA. However, this
method can cause diverse blocking effects on other miRNAs and this technique showed
mild anti tumor effect in vitro experiments (19), suggesting that this is not a practical
approach to inhibit miRNA expression. For replacement of miRNA in cancer with
reduced miRNA expression, miRNA mimetics and miRNA expressing vectors could be
used.
Although miRNA-based therapeutics is promising, their effective delivery to
specific tissue is a critical issue that needs to be addressed.
1.6.1 Inhibition of miRNA using antisense oligonucleotides (ASO)

For the past 20 years, the antisense field has progressed to better understand the
mechanism of action, delivery, pharmacokinetics, chemical modifications and function of
antisense (119). Antisense is 15-20 nts that binds to target RNA through Watson-Crick
base-pairing. There are several possible mechanisms of action for antisense. Antisense
may bind to the target RNA inhibiting translation (120, 121) or modulating pre-mRNA
splicing (122). Sense-antisense (RNA-DNA) duplex recruits an endogenous nuclease,
RNase H, which degrades only the target RNA (119).
15
Chemically
modified
antisense with 2 sugar modifications and phosphorothioate backbone modification

showed effective inhibition of miRNAs (123-126) (Figure 2). For 2 sugar modifications,
2-O-methyl (2-OMe), 2-O-methoxyethyl (2-MOE), 2-flouro (2-F) and locked
nucleic acid (LNA) are used in vitro. The modified antisense shows improved activity,
stability and delivery. Other modifications to antisense also enhance the silencing effect.
For example, antisense to miR-122 was linked with cholesterol at the 3 end. The socalled Antagomirs produced sustained knock down of miR-122 in mouse liver up to 23
days after injection (127). Inhibition of miR-21 in glioblastoma cells and inhibition of
miR-125b-1 in HeLa cells and PC3 cells results in apoptosis and decreased cell viability
(87, 128). These data suggest that antisense may be used to inhibit miRNA and that it
can be used as a potential therapy in cancer treatment.
However, antisense may have off-target effects. Antisense and random target
genes may bind to each other producing an unexpected non-target effect. One paper
demonstrated that phosphorothioate backbone modification showed non-antisense effects
through binding of cellular proteins (129). To measure the off-target effects of antisense,
several mismatch control oligos containing the same nucleotide composition as the
antisense should be tested and selection of good control oligonucleotides, better
modification and alternative delivery method might reduce these problems.
1.6.2. Replacement of miRNA

In situations of reduced miRNA expression in certain diseases, re-introduction of
the miRNA could be advantageous. One method of re-introducing miRNA is through
miRNA mimetics or miRNA expressing vectors. miRNA mimetics contain endogenous
16
mature miRNA sequence in a duplex structure.
The duplex structure is necessary
because RISC is more efficient at loading small RNAs from double-stranded RNAs
rather than a single-stranded RNAs (130). RISC will also process the duplex to release
the single stranded mature miRNA.
miRNA mimetics successfully inhibit Gli-1
expression and modified miRNA mimetics could show better effect than endogenous
miRNA (131).
An alternative method to replace miRNA is through the use of short hairpin RNA
(shRNA) vectors. This vector can maintain silencing effect for a long period of time and
more than one miRNA transcripts can be expressed from the same vector. shRNA may
introduce enough pre-miRNA, to cause saturation of the Exportin-5 pathway (132).
Modified adenovirus have been used, but problems with immune response to the
adenovirus and available tissues that virus can efficiently infect limit the usage of this
virus (133). Lentivirus also have been studied for shRNA vector and this produces more
stable and persistent silencing effect because it incorporates into the genome (134).
However, lentivirus shows the possibility of insertional mutagenesis and wild-type virus
production (134).
1.7 OJECTIVE OF DISSERTAION
The first aim of this study is to identify the most important miRNAs that are
deregulated in pancreatic cancer. Theoretically, these deregulated miRNAs may be used
for diagnosis or treatment of pancreatic cancer. The expression of miRNA precursors
was investigated in pancreatic tissues and cancer cell lines (Chapter 2). Overexpressed
17
miRNAs in pancreatic cancer were inhibited using antisense oligonucleotides (Chapter 3

and 4) and the effects of combining antisense oligonucleotides to miRNA and commonly
used a chemotherapeutic agent was studied (Chapter 3).
The target molecules of
miRNAs that are deregulated in pancreatic cancer were studied in Chapter 4.

The second aim was to see if the processing of miRNA differed in various normal
tissues, cancer cell lines and in patients tumor tissue (Chapter 5).
18
Pol II
miRNA gene
Pri-miRNA
cap
AAA.A
Drosha/DGCR8
Pre-miRNA
Nucleus
Exportin-5
Dicer
Dicer/TRBP/Ago
PremiRNA
Cytoplasm
Mature miRNA in RISC
Figure 1.1. miRNA biogenesis.
19
Figure 1.2. Chemical modification of antisense oligonucleotides.

(A): 2-MOE (2-O-methoxyethyl), (B): 2-OMe (2-O-methyl), (C): 2-F, (D): LNA,
(E): phosphorothioate backbone, (F): phosphodiester backbone.
20
CHAPTER 2
EXPRESSION PROFILING IDENTIFIES MICRORNA SIGNATURE IN

PANCREATIC CANCER
2.1 INTRODUCTION
Pancreatic cancer is the fourth leading cause of cancer-related death in the United
States (135). The annual death rate over the last five years has been ~30,000 with a
similar number of new cases diagnosed each year. The prognosis for pancreatic cancer is
the worst of all cancers with a mortality/incidence ratio of 0.99 (136). The incidence of
pancreatic cancer in the United States is ~9 per 100,000 (137). These discouraging
numbers, reflecting the increasing rates of incidence and death, are due to the lack of
improvement in detection and diagnosis strategies and the paucity of breakthroughs in
treatment regimens.
A growing number of direct and indirect evidence suggests a relationship between
altered miRNA expression and cancer. These include miR-15a and miR-16-1 in chronic
lymphocytic leukemia (56, 113), miR-143 and miR-145 in colorectal cancer (76), let-7 in
lung cancer (79, 97) and miR-155 in diffuse large B cell lymphoma (91). Expression
profiling has identified other cancers with differential expression of several miRNAs,
21
including breast cancer (99), glioblastoma (87, 100) and papillary thyroid cancer (90). A
polycistron encoding five miRNAs is amplified in human B-cell lymphomas and forced
expression of the polycistron along with c-myc was tumorigenic, suggesting that this
group of miRNAs may function as oncogenes (94, 95).
The purpose of this study was to profile the miRNA expression in clinical
specimens of pancreatic adenocarcinoma. A real-time, quantitative PCR assay (59, 61)
was used to profile the expression of over 200 miRNA precursors in clinical specimens of
pancreatic cancer and pancreatic cancer cell lines. A unique miRNA signature was
identified that distinguished pancreatic cancer from normal and benign pancreas.
2.2 MATERIAL AND METHODS
2.2.1 Tissue procurement

The tissue samples analyzed in this study were derived from patients undergoing a
surgical procedure to remove a portion of the pancreas at the University of Oklahoma
Health Sciences Center and The Ohio State University.
The collection of samples
conformed to the policies and practices of the facilitys Institutional Review Board.
Upon removal of the surgical specimen, research personnel immediately transported the
tissue to the surgical pathology lab. Pathology faculty performed a gross analysis of the
specimen and selected cancerous appearing pancreatic tissue and normal appearing
pancreatic tissue for research. Each sample was placed in a cryovial and flash-frozen in
liquid nitrogen and stored at -150C until analysis. Subsequent pathologic analysis by
the institutes providing the surgical specimens confirmed the histopathology of the
22
samples taken for research. A second level of quality control was performed on the
adjacent benign tissues by the laboratory that performed the RNA analysis. Histological
slides were prepared from the section of the frozen tissue directly adjacent to tissue from
which RNA was isolated.
These slides were examined by one of us (W.L.F.) to
determine if the benign tissues contained any pancreatic tumor cells. Benign tissue that
contained residual tumor was not included in the study.
The clinical data on the
specimens are listed in Table 2.1.
2.2.2 Cell lines

The following pancreatic tumor cell lines were purchased from American Type
Tissue Collection (Manassas, VA), which are Panc-1, HS766T, MIA PaCa-2, HPAF-II,
BxPC-3, Mpanc-96, PL45, Panc03.27 and Panc10.05. Cell lines were cultured in RPMI
1640 medium with 10% FBS or other optimized complete medium using standard
conditions.
2.2.3 miRNA precursor expression profiling

Total RNA was isolated from the cell lines or tissues in 1 ml of Trizol (Invitrogen,
Carslbad, CA). Frozen tissues (~10 mg) were first pulverized in a stainless steel mortar
and pestle. Total RNA from normal pancreases were purchased from Ambion (Austin,
TX), BD Biosciences (Mountain View, CA) and Stratagene (La Jolla, CA). All donors of
the normal tissue died from complications other than pancreatic diseases (Table 2.1).
RNA concentration was determined by analyzing 1 l of solution using the ND-1000
micro-spectrophotometer (NanoDrop Technologies, Wilmington, DE). RNA integrity
23
was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
RNA integrity number (RIN) was determined using the RIN algorithm of the Agilent
2100 expert software (138) (Table 2.1). RNA with a RIN 4 was included in the study.
RNA was briefly treated with RNase-free DNAase I and cDNA was synthesized from 1
g of total RNA using gene specific primers to 222 miRNA precursors plus 18S rRNA as
described (59, 61). The expression of 222 miRNA precursors was profiled using a realtime quantitative PCR assay (59, 61). Duplicate PCRs were performed for each miRNA
precursor gene in each sample of cDNA.
The mean CT was determined from the
duplicate PCRs. Relative gene expression was calculated as 2
(C
C
TmiRNA
T18SrRNA).
Relative gene expression was multiplied by 106 to simplify the presentation of the data.
The raw relative miRNA precursor expression data are provided in Appendix Table A.
2.2.4 Real-time PCR of mature miRNA

Assays to quantify the mature miRNA (i.e. TaqMan microRNA Assays, Applied
Biosystems Foster City, CA) were conducted as described (60) with one modification. A
5X cocktail containing 28 different antisense looped RT primers was prepared by
concentrating the 2X stock solutions in a Speed Vac. One hundred nanograms of total
RNA was heated for 5 min at 80C and then incubated for 5 min at 60C with 10 M of
the 18S rRNA antisense primer followed by cooling to room temperature.
Three
microliters of the 5X looped primer mix was then added and the cDNA was made as
described (60). This allowed for the creation of a library of 28 miRNA cDNAs plus the
18S rRNA internal control. Real-time PCR (10 l total reaction) was performed as
described (60) using 1 l of a 1:50 dilution of cDNA. Duplicate PCRs were performed
24
for each mature miRNA gene in each sample of cDNA. The mean CT was determined
from the duplicate PCRs. Gene expression was calculated relative to 18S rRNA as
described earlier and multiplied by 106 to simplify data presentation.
2.2.5 Northern blotting

Northern blotting was performed as previously described (2, 59).
DNA
oligonucleotides of the reverse compliment to the mature miRNA were used as probes.
Blots were successfully stripped and reprobed up to 3 times.
2.2.6 RT in situ PCR

The RT in situ PCR protocol was performed as previously described (139). Briefly,
optimal protease digestion time was determined using nonspecific incorporation of the
reporter nucleotide digoxigenin dUTP. Optimal protease digestion was followed by
overnight incubation in RNase-free DNase (10 U per sample, Boehringer Mannheim,
Indianapolis, IN) and one step RT/PCR using the rTth system and digoxigenin dUTP.
The chromogen is nitroblue tetrazolium and bromochloroindolyl phosphate (NBT/BCIP)
with nuclear fast red as the counterstain. The primer sequences to the precursors of miR221, miR-301 and miR-376a were the same as those used for the profiling (61). The
negative controls included omission of the primers and substitution with irrelevant
(human papillomavirus specific) primers, as this virus does not infect pancreatic tissue.
RT in situ PCR was performed on the archived, formalin-fixed paraffin-embedded
sample 1050005A2(T) (Table 2.1).
25
2.2.7 Statistics
The CT data for 201 gene expression values (relative to control gene expression)
were mean-centered and analyzed using the following strategy. The expression patterns
of unfiltered data were assessed using unsupervised hierarchical clustering of samples
and unsupervised hierarchical clustering of genes based on average linkage and Euclidian
distance (140, 141). To determine genes that are differentially expressed between groups
of samples, the data were filtered on significance of differences using multi-group
permutations-based ANOVA test (Welch approximation) with p < 0.01 (10,000 random
permutations) and multiple testing correction (Westfall-Young step-down correction with
maxT). To compare the expression patterns of differentially expressed genes, the filtered
data were analyzed using hierarchical clustering of samples and hierarchical clustering of
genes based on average linkage and Euclidian distance (140, 141).
Additional cluster analysis of filtered data was done using an expression terrain
map (142). Terrain maps provide a three-dimensional overview of the major clusters
inherent in the data. Samples were first mapped into a two-dimensional grid in which the
placement of each element is influenced by a number of nearest neighbors based on
Euclidian distance that was calculated using miRNA precursor expression data. The third
dimension is determined by the density of points over the two-dimensional grid and its
value is projected as a surface; higher peaks indicate larger numbers of very similar
elements. The average correlation between each pair of samples was also calculated.
Each pair of samples with an average correlation above the threshold (0.8) is indicated on
the expression terrain map by a line connecting the two samples. This allows visualizing
subsets of samples with a highly correlated pattern of miRNA expression.
26
Peaks
representing samples from a particular cluster are labeled with color coded spheres on top
of each peak.
A supervised machine learning algorithm was applied for classification of
samples based on unfiltered miRNA expression data. The predictive scores for each
miRNA were calculated based on 2 class comparison (normal vs. tumor) of expression
data using the prediction analysis of micro-arrays algorithm (PAM) (143) based on
training, take-one-out cross validation and testing procedures.
The division of the
samples into training and test sets is done using the commonly accepted approach of
randomly splitting data into training and test sets (usually 2/3 for train, 1/3 for test) (143).
For this study, tumor samples were randomly split into ~75% for training and 25% for
testing. Since the number of normal cases was small (N = 6), normal cases were included
in the training set only. The small number of chronic pancreatitis samples (N = 4) were
not included in the PAM analysis. While, two levels of quality control were performed to
eliminate adjacent benign tissue that contained any tumor cells, the RNA was extracted
from whole tissue rather than microdissected tissue. Thus, we could not completely rule
out the possibility that some tumor cells contaminated the adjacent benign, nor do we
know if early premalignant changes have occurred in these benign samples that are
obtained from tissue adjacent to tumor. Therefore benign samples were excluded from
the training set in order to train the classifier on true normal and tumor cases. Benign
cases were used in the test set.
27
2.3 RESULTS
2.3.1 Validation
Pancreatic tissue is rich in ribonuclease and care must be taken during RNA
isolation to reduce the possibility of autolysis. To validate the integrity of the RNA
isolated from the pancreatic tissue, ~100 ng of each RNA sample was assayed using the
Agilent 2100 Bioanalyzer. Fifty-two tissues had a RIN 4 (median 7.6, range 4.39.6,
Table 2.1).
miRNA precursor expression in each of the samples was determined using realtime PCR and normalized to an internal control gene. Since equivalent amounts of total
RNA was added to each RT reaction, 18S rRNA was validated as the internal control by
comparing the mean expression among the various samples (i.e. tumor, normal and
benign).
There was no statistically significant difference in the mean 18S rRNA
expression between the tumor samples or the normal pancreas (p = 0.116, Figure 2.1).
The 18S rRNA expression in the tumor and normal tissue determined here are in
agreement with those previously reported in pancreatic tissue (144). Since there was no
significant difference in the 18S rRNA expression between the tumor, normal and benign
groups, 18S rRNA was selected as the internal control gene in the study.
2.3.2 miRNA precursor profiling in pancreatic tissues

The expression of 201 miRNA precursors, representing the 222 miRNAs
discovered as of April, 2005 was profiled in 28 tumors, 15 adjacent benign tissues, 4
chronic pancreatitis specimens, 6 normal pancreas tissues and 9 pancreatic cancer cell
28
lines. Unsupervised hierarchical clustering of samples was performed on the entire set of
unfiltered data. The heatmap demonstrates that unfiltered expression data of only 201
miRNA precursors sufficiently sorts the samples into clusters of normal pancreas, tumor
and cell lines (Appendix Figure A). While most of the benign samples clustered together
with the normal pancreas samples, several benign samples clustered with the tumor
samples or pancreatitis.
The miRNA precursor expression data was filtered using multigroup ANOVA.
Statistical filtering of data is conventionally considered to be a required step of the
expression data preprocessing because gene expression data are inherently noisy.
Hierarchical clustering of samples and genes was performed on the resulting 112
miRNAs. Hierarchical clustering of filtered data allowed us to identify major groups of
miRNAs that have different patterns of expression in the resulting four clusters of
samples (Figure 2.2 a,b).
One cluster contained only cell lines.
Another cluster
contained all 6 normal pancreases and 9 of 15 benign tissues. A third cluster contained
the 4 chronic pancreatitis specimens and 1 benign tissue.
Finally, a large cluster
contained 28 of 28 tumors and 5 benign tissues (Figure 2.2 a,b).

The filtered data were also analyzed using a different clustering technique known
as expression terrain maps (142). The terrain map separated the expression data into 5
main groups of samples (Figure 2.3). Like the hierarchical clustering, the terrain map
separates groups of samples from the normal pancreas, the cell lines, pancreatic
adenocarcinomas and chronic pancreatitis. This analysis showed that each of the clusters
of samples occupies distinct regions on the expression terrain map thus providing
additional evidence that each of the 4 groups of samples has distinct patterns of miRNA
29
expression and suggests the possibility of finding subsets of microRNAs that discriminate
normal and tumor samples. Most of the adjacent benign tissue grouped in between the
normal and tumor, while those benign samples that clustered with the tumor also grouped
with the tumor on the terrain map (Figure 2.2 and 2.3). As an additional feature of terrain
map, the average correlations of all miRNA expression values were calculated between
each pair of samples. Those correlations above the threshold of 0.8 are shown as lines
connecting pairs of samples with similar patterns of miRNA expression. This allows
visualizing subsets of samples with a highly correlated pattern of miRNA expression.
The samples within each of the 4 main groups are connected (i.e. average correlation
>0.8) but there were no between the groups connections, except for two pancreatitis
samples that correlated with some of the tumor samples (Figure 2.3).
Comparing the gene expression profile among different pancreas tissues has been
used to eliminate the stroma and cell proliferation (e.g. cell line) contributions and
identify genes expressed in pancreatic tumors (145-147). An attempt to perform such
analysis on the miRNA expression data in Figure 2.2a was unsuccessful due to the
uniformly high expression in the cancer cell lines and the uniformly low expression in the
pancreatitis samples.
However, a number of possible tumor-related miRNAs were
identified that were increased in the cell lines or tumors but not in the normal or
pancreatitis (Figure 2.2a).
2.3.3 Data analysis by prediction analysis of micro-array algorithm (PAM)

The PAM classification algorithm was used to determine if the miRNA
expression data could predict which class the samples fit (tumor or normal) and to
30
determine the most important, differentially expressed miRNAs related to pancreatic

adenocarcinoma. The unfiltered data on 201 pre-miRNAs were analyzed by the PAM
algorithm (143). The 3 genes with more than 75% of missing data were eliminated from
the analysis in order to prevent possible artifacts of the imputation algorithm. PAM
training and cross-validation were conducted using the 6 normal pancreas and 18
pancreatic tumors. PAM has correctly classified 100% of the normal and tumor samples
(Figure 2.4a). PAM testing was conducted on 10 tumor samples and 15 adjacent benign
samples. PAM has correctly classified 100% of the tested tumor samples and 11 of 15 of
the tested benign samples (Figure 2.4b). The 20 top ranked differentially expressed
miRNAs in pancreatic adenocarcinoma as selected by PAM are listed in Table 2.2.
2.3.4 Validation of miRNA expression

The real-time PCR assay used in this study quantifies the miRNA precursors and
not the active, mature miRNA (59, 61). Northern blotting was performed on the identical
RNA used in the real-time PCR analysis to validate if the mature miRNA correlated with
the precursor. Expression levels of mature miR-100, mir-375and miR-155 from 4 normal
pancreases and 5 pairs of tumor/adjacent benign tissue paralleled the miRNA precursor
levels by PCR (Figure 2.5). miR-100 and miR-155 were among the top 20 differentially
expressed miRNAs (Table 2.2). miR-375 was validated by Northern blotting because it
was one of the few miRNAs with decreased expression in pancreas cancer. While miR375 was not among the top 20 miRNAs (Table 2.2), the expression of both precursor and
mature miR-375 was significantly decreased in pancreas cancer by real-time PCR (p < 1
X 10-5). miRNA expression in the paired benign and tumor tissue was consistently
31
increased or decreased in all cases, demonstrating that the differences in miRNA

expression between tumor and benign are due to differences in individual patients tissues
and not due to differences in the mean expression of the group.
The real-time PCR data was further validated using a commercially available realtime PCR assay to amplify and quantify the mature miRNA. Mature miRNA expression
was validated on 8 of the top aberrantly expressed miRNAs from PAM. cDNA from the
following tissues were assayed: 6 normal pancreases, 16 pancreatic adenocarcinomas and
10 adjacent benign tissues that were predicted as normal from PAM. The mature miRNA
expression highly correlated with the miRNA precursor (Figure 2.6). To our knowledge,
this is the initial presentation of both precursor and mature miRNA expression
determined by sensitive, real-time PCR assays.
A direct comparison between the
precursor and mature PCR data presented in Figure 2.6 is not possible. Among other
reasons, different amounts of RNA were added to each RT, 1 g for the precursor assay
and 100 ng for the mature. Despite these differences, the relative amount of mature
miRNA is greater than the precursors in all cases except for panel H. This demonstrates
that the steady state levels of mature miRNA are more predominant than the precursors, a
fact that is documented on nearly all published Northern blots that show the band
intensity of the mature is more intense than the precursor. We have no explanation as to
why this situation is apparently reversed in the case of panel H (miR-212). Of interest is
that while the relative expression values for these mature miRNAs spanned 3-logs (from
0.1 to 100), the trend in differential expression between the tumor and normal tissues
remained (Figure 2.6).
This suggests that miRNAs function in tumor and normal
32
pancreas at different expression levels, yet the differential expression is maintained

between cancer and normal tissue.
Finally, the cell-type miRNA expression was studied using RT in situ PCR. miR221, miR-376a and miR-301 were selected for the in situ PCR since they were among the
top differentially expressed miRNAs (Table 2.2) and had increased expression in the
tumor and cell lines compared to the normal pancreas and pancreatitis (Figure 2.2). We
were particularly interested in the cell type expression of miR-376a since it was cloned
from pancreas cells (67). miR-221 and miR-376a are localized to the tumor cells and not
to the benign pancreatic acini or stromal cells (Figure 2.7) or benign ducts (not shown).
miR-301 was also localized to tumor (data not shown).
2.4 DISCUSSION
Reported here are the results of the first detailed miRNA expression profiling
study in pancreatic ductal adenocarcinoma.
Expression profiling identified a large
number of miRNAs that are aberrantly expressed in pancreatic ductal adenocarcinoma.

A bead-based flow cytometric assay was used previously to profile the expression of 217
miRNAs in nine samples of pancreatic adenocarcinoma (65). A micro chip assay was
recently used to profile the expression of miRNAs in 39 samples of endocrine pancreas
cancer (148). Our results in pancreatic ductal adenocarcinoma more closely approximate
those of Volinia, et al. (148), in endocrine pancreas tumors and show that the majority of
miRNAs are increased in the tumor compared to normal pancreas. Many of the miRNAs
that are increased in both pancreas adenocarcinoma and endocrine pancreas cancer are
33
similar, including miR-221, -100, -125b and -21. On the other hand, Lu, et al. (65),
report an almost universal decrease in miRNA expression in the pancreatic
adenocarcinomas compared to normal pancreas.
miRNAs are believed to function primarily as negative regulators of gene
expression following binding to conserved sequences within the 3 UTR of target
mRNAs. While the biological roles of miRNA are under intense investigation, they are
believed to define and maintain cellular fate in a manner similar to transcription factors
(149) by regulating developmental timing and differentiation (150). Since alterations in
developmental pathways play a critical role in pancreatic cancer development (151, 152),
alterations in miRNA expression may be an important contributor to the development of
pancreatic adenocarcinoma.
Our study is unique in that a sensitive, real-time PCR assay was used to profile a
relatively small number of nonconding RNAs. Real-time PCR is the gold standard of
RNA quantification and has much less technical noise and greater reproducibility than
traditional cDNA micro-arrays. miRNA expression profiling correctly identified 28 of 28
tissues as tumor (Figure 2.4). All 6 normal pancreases were correctly predicted and 11 of
15 adjacent benign tissues were classified as normal tissue (Figure 2.4).
The data
presented here reinforces the argument that miRNA expression profiling may generate a
unique molecular signature for a given cancer. This concept is supported by several
recent studies.
Profiling of various cancers revealed that the pattern of miRNA
expression varies markedly across different tumors and that a small number of miRNAs
define the cancer better than expression data from 16,000 mRNAs (65). A unique
expression signature of only 13 miRNAs differentiated cases of the more aggressive form
34
of chronic lymphocytic leukemia from the more indolent form and was associated with
the presence or absence of disease progression (153).
The 3 factors that are likely driving the differences in gene expression are the
normal acini, stroma and tumor cells. We cannot conclude that each of the aberrantly
expressed miRNAs (Table 2.2) reflect a difference in expression between normal ductal
epithelium and tumor and may reflect differences in expression among the different cell
types (stoma, acini and tumor). However, we confirmed by RT in situ PCR that 3 of the
top differentially expressed miRNAs that were identified in the screen are localized to the
tumor cells (Figure 2.7). We cannot explain why some of the benign tissues failed to
cluster with the normal pancreas (Figure 2.2 and 2.3). While 2 levels of quality control
were used to reduce the possibility of contaminating tumor cells, it is possible that some
tumor cells were present in the benign tissue since the RNA was isolated from whole
tissue and not microdissected tissue. Another possibility is that premalignant changes
have already occurred in some of the benign tissues as those samples are obtained from
tissue adjacent to tumor. It is interesting that most of the benign samples (and chronic
pancreatitis as well) lie in between the normal pancreas and tumor on the expression
terrain map (Figure 2.3).
This observation may indeed describe the premalignant
alterations that have occurred in these benign tissues. Future studies using RT in situ
PCR and perhaps laser microdissection will be able to address these issues in more detail.
Some of the differentially expressed miRNAs in pancreatic cancer were aberrantly
expressed in other cancers. These include miR-155, which was increased in the present
study and in diffuse large B-cell lymphoma (91); miR-21 was increased here and in
glioblastomas (87, 100), breast cancer (99) and papillary thyroid cancer (90); miR-221
35
was increased in pancreatic cancer, in glioblastoma (100) and in thyroid cancer (90).
miR-221 is located ~700 bp from miR-222 on the X chromosome; both miR-221 and
miR-222 are predicted to bind to and regulate kit (90). miR-222 precursor was not
among the top 20 differentially expressed miRNAs (Table 2.2); however, subsequent
analysis of mature miR-222 by PCR showed that miR-222 was increased in pancreas
cancer at levels that were similar to miR-221 (data not shown). Thus, deregulation of the
miRNAs mentioned earlier may be unique to cancer in general. miRNAs differentially
expressed in other cancers were not deregulated to the same degree in pancreatic cancer.
The let-7 family, decreased in lung cancer (79, 97), was increased here. Expression of
the miR-17-92 polycistron (encoding miR-17, -18, -19a, -19b-1 and -92-1) was increased
in lymphoma and colorectal cancer (94) but was not significantly altered in pancreatic
cancer. We report deregulation of a number of miRNAs in pancreatic cancer such as
miR-376a and miR-301 that have not been reported in any other cancers to our
knowledge. Also of interest is the fact that most of the deregulated miRNAs reported
here show increased expression in the tumors compared to the normal pancreas. A few
miRNAs had reduced expression in pancreatic cancer including miR-375 (Figure 2.5).
miR-375 was cloned from pancreas and is believed to be islet cell specific (67).
We report significant changes in miRNA expression between pancreatic
adenocarcinoma and normal pancreas.
Since each miRNA may regulate scores of
mRNAs (45), the impact on gene expression in pancreatic cancer may be profound. The
miRNA field is currently hampered by a lack of methods to sort through the many
hundreds of predicted miRNA target genes. As more sophisticated approaches become
36
available to identify and validate miRNA targets, the role of aberrant miRNA expression
in pancreas cancer will become better understood.
2.5 ACKNOWLEDGEMENTS
Supported by an NIH grant CA107435 to T.D.S. The Tissue Procurement Shared

Resource at The Ohio State University is funded by the National Cancer Institute, grant
P30 CA16058. We thank Dr. Caifu Chen at Applied Biosystems for his assistance with
the TaqMan looped primer assays.
37
Figure 2.1. 18S rRNA expression in pancreatic tissue.

The expression of the 18S rRNA internal control is shown in pancreatic tumors, adjacent
benign tissue, normal pancreas, chronic pancreatitis and pancreatic cancer cell lines. 18S
rRNA expression, determined using real-time PCR as described in Material and Methods,
is presented as 2-CT. Dashed line, mean value.
38
Figure 2.2. miRNA precursor expression in pancreatic samples.

(a) The relative expression of each miRNA precursor was determined by real-time PCR;
data are presented as CT. Hierarchical clustering was performed on a subset of 112
genes that are differentially expressed (p < 0.001) among groups (tumor, chronic
pancreatitis, cell lines and normal tissue) as determined by ANOVA multi-group
comparison test. A median expression value equal to 1 was designated black; red,
increased expression; green, reduced expression; grey, undetectable expression.
(b) Dendrogram representing the results of hierarchical clustering analysis of the miRNA
precursor expression pattern in 62 samples. Samples include primary pancreatic tumors
(N = 28), normal pancreatic tissues (N = 6), adjacent benign pancreas (N = 15), chronic
pancreatitis (N = 4) and pancreatic cancer cell lines (N = 9).
39
Figure 2.3. Three-dimensional expression terrain map.

It was created from the filtered miRNA precursor expression data presented in Figure 2.2
(a). Each mountain represents an individual sample (tumor, adjacent benign, chronic
pancreatitis, normal pancreas or pancreatic cancer cell line). The individual mountains
sort into small groups based upon their similarities or differences to each other. Colored
dots represent the same clusters as in Figure 2.2 (b). Black dots represent the 6 normal
pancreases. The lines connecting pairs of samples indicate those samples which have
very similar patterns of miRNA expression with average correlation above the threshold
(>0.8).
40
Figure 2.4. Estimated probabilities for the training and test data.
All training data including 6 normal pancreas samples and 18 of the samples known to be
pancreatic tumors are correctly classified (a). Eleven out of 15 adjacent benign samples
and 10 samples known to be pancreatic tumors are correctly classified in the testing
group (b). Samples are partitioned by the true class (a) and the predicted class (b).
41
Figure 2.5. miRNA expression by Northern blotting.

The expression of miR-100, miR-375 and miR-155 was determined in tissue specimens
of pancreatic cancer (T), adjacent benign tissue (B) or normal pancreas (N). Blots were
stripped and reprobed. tRNA, visualized by ethidium bromide staining, was used as a
loading control.
42
Figure 2.6. Validation of precursor and mature miRNA levels.

The expression of 8 miRNAs was validated in 6 normal pancreas specimens, 10 adjacent
benign tissues and 16 pancreatic adenocarcinomas. The relative expression of the
miRNA precursors (open bars) was determined using a real-time PCR assay to the
miRNA precursors while the relative expression of the mature miRNA (closed bars) was
determined using a real-time PCR assay to the mature miRNAs. The mean differences in
miRNA expression between the normal pancreas (black) and tumors (red) was significant
p < 0.01 (students t-test). (a) let-7i, (b) miR-221, (c) miR-100, (d) miR-301, (e) miR-21,
(f) miR-181a,c (precursor) and miR-181a (mature), (g) miR-125b-1 (precursor) and miR125b (mature), (h) miR-212.
43
44
Figure 2.7. Histologic and molecular analyses of pancreatic cancer for microRNA
expression.
Panel (a) (X400) depicts the hematoxylin and eosin analysis of a pancreatic
adenocarcinoma. The normal pancreatic glands (small arrow) are being invaded by the
poorly formed glands of the carcinoma (large arrow). Serial section analysis of miR-221
after in situ amplification of the corresponding cDNA showed that many of the tumor
cells contained the target sequence; note the cytoplasmic localization (arrows, panel (b)
X 400 and at higher magnification, panel (c) X 1,000; the signal is blue due to
NBT/BCIP with negative cells counterstained with fast red). The signal was lost with
either omission of the primers or substitution with HPV-specific primers (panel (d),
X400). The adjacent serial section also showed many of the tumor cells expressed miR376a after in situ amplification of the cDNA (e, f). Panel (e) (X400) shows the positive
tumor cells (large arrow) and the negative stromal cells in the areas of desmoplasia (small
arrow) while panel (f) (X400) depicts the positive tumor cells (large arrow) adjacent to
the negative benign pancreatic gland acini (small arrow).
45
Sample No.
N1
N2
N3
N4
BD
ST
Sample No.
OUP2
OUP14
OUP16
OUP20
OUP27
OUP29
OUP28
OUP31
OUP33
OUP36
OUP37
OUP42
OUP48
OUP49
OUP91
OUP97
OUP101
OUP105
OUP122
OUP123
OUP135
OUP139
0206C077B(T)
0206C138B(T)
1050005A2(T)
3030800A3(T)
3030396A1(T)
3030401A1(T)
Supplier
Ambion
Ambion
Ambion
Ambion
Clontech
Stratagene
Normal Pancreas Tissues

Age (years)/Gender
Cause of Death
82 / Female
Emphysema
20 / Male
Head Trauma
71 / Male
Cerebral Vascular Accident
71 / Male
Failure to Thrive
35 / Male
Sudden death
72 / Male
Chronic Obstructive Pulmonary Disease
Pancreatic Adenocarcinoma Tissues

Differentiatio
Age
Tumor Pathology
n
(years)/Gender
T3NxMx
Moderately
85 / Male
T3N0Mx invasive
Poor-moderate
71 / Male
T3N1bMx
Well
50 / Male
T3N1aMx
Poorly
70 / Male
T3N0Mx invasive
Moderate
86 / Male
T3N0Mx invasive
Poorly
63 / Male
T3N1bMx invasive Poorly
48 / Female
T3N0Mx
Poorly
38 / Male
T3N1Mx
Poor-moderate
42 / Female
T3N1bMx
Moderate
56 / Female
T3N1bMx invasive Mod-poor
79 / Male
T3N0Mx invasive
Moderate
78 / Female
T3N1bMx invasive Moderate
61 / Male
T3N1aMx invasive Poor-moderate
62 / Female
T3N1bMx
Moderate
65 / Male
T3N1bMx
Moderate
56 / Male
T2N0Mx invasive
Moderate
52 / Female
T2N1aMx
Moderate
55 / Male
T3N1aMx
Moderate
64 / Male
T3N1bMx
Moderate-well
52 / Male
T3N0Mx
Moderate
82 / Male
T2N0Mx
Moderate
50 / Male
T3N0Mx
Moderately
49 / Male
Intermediate to
T3N0M1
44 / Female
high grade
T3N1Mx
Poorly
73 / Female
T3N1Mx
Moderately
46 / Male
Intraductal
59 / Male
papillary mucinous tumor
Intraductal
72 / Male
papillary mucinous tumor
Table 2.1. Clinical data and tumor pathology.
46
RINa
9.6
7.8
9.2
9.1
7.5
6.8
RIN
(tumor)
8.2
7.8
5.6
8.2
3.3
8.3
7.9
7.7
5.1
7.7
7.1
6.6
8.1
7.6
5.9
8.0
8.5
8.3
9.1
6.4
7.8
6.6
7.2
RIN
(benign)
7.0
3.8
5.6
3.4
5.8
7.0
6.9
7.0
8.3
7.8b
No tissue
7.6
4.3
8.6
6.1
2.6
6.6
8.9
8.4
7.6 b
8.2 b
6.7
No tissue
6.1
No tissue
7.0
7.5
7.3
No tissue
7.3
No tissue
8.9
No tissue
Rank
Name
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
miR-221
miR-424
miR-301
miR-100
miR-376
miR-125b-1
miR-021
miR-345
miR-016-1
miR-181a,c
miR-092-1
miR-015b
miR-142-P
miR-155
let-7f-1
miR-212
miR-107
miR-024-1,2
let-7d
miR-139
p-value
(t-test)
5.66E-05
3.62E-08
1.11E-05
4.40E-06
7.00E-04
1.00E-04
2.00E-04
1.44E-15
3.73E-04
8.31E-04
3.40E-03
4.00E-04
3.63E-07
1.51E-03
4.00E-04
2.00E-04
3.86E-05
9.12E-08
7.06E-04
6.79E-11
Fold
Change
26.2
56.3
34.2
36.9
7.79
23.2
15.7
-14.5
14.3
18.6
19.6
8.55
-15.4
14.0
10.9
22.2
8.20
8.17
8.38
-7.91
Chromosome
location
Xp11.3
Xq26.2
17q23.2
11q24.1
14q32.31
11q24.1
17q23.2
14q32.2
13q14.2
9q33.3, 19p13.13
13q31.3
3q25.33
17q23.2
21q21
9q22.32
17p13.3
10q23.31
9q22.32, 19p13
9q22.32
11q13.4
Table 2.2. Top 20 aberrantly expressed miRNA precursors in pancreatic

adenocarcinoma.
47
CHAPTER 3
ANTISENSE INHIBITION OF microRNA REDUCES CELL VIABILITY AND

SENSITIZES THE EFFECT OF GEMCITABINE IN PANCREATIC CANCER
3.1 INTRODUCTION
Pancreatic cancer is the fourth leading cause of cancer-related death in the United
States (135). The annual death rate over the last five years has been approximately
30,000 with a similar number of new cases diagnosed each year. The prognosis for
pancreatic cancer is the worst of all cancers with a mortality/incidence ratio of 0.99. The
incidence of pancreatic cancer in the United States is approximately 9 per 100,000 (136,
137). Pancreatic ductal adenocarcinoma (PDA) is the most common and lethal type of
pancreatic cancer. The reasons for the poor survival rate are a lack of effective diagnostic
procedure to identify pancreatic cancer and a lack of effective treatments for the disease.
Normal pancreatic tissue consists primarily of acinar cells, which produce
digestive proenzymes, islets, which produce hormones that control blood sugar such as
insulin and glucagon, and a low number of ductal epithelial cells. However, if pancreatic
tissue turns to tumor, the major cell type is neoplastic ductal epithelial cells and stroma
cells.
48
Not many sensitive and specific biomarkers of pancreatic cancer are available.
Currently serum maker CA19-9 (sialylated Lewis blood group antigen) is used, but the
usage is limited because it showed the high false-positive rate and some patients do not
express Lewis antigens (154-156). Recently, serum macrophage inhibitory cytokine 1
(MIC-1) seems to be a better marker of pancreatic cancer than CA19-9 (157). Genetic
and epigenetic markers also can be used for early detection. Pancreatic cancer is caused
by genetic alterations such as chromosomal instability or point mutation of oncogenes
and tumor-suppressor genes. Chromosomal changes in pancreatic cancer have been
identified using karyotyping, comparative genomic hybridization, and allelotyping (158).
Chromosome losses are more common than gains and poorly differentiated ductal
adenocarcinoma showed more frequent karyotypic abnormalities than well-differentiated
pancreatic cancer (159). The specific oncogenes and tumor suppressor genes are also
altered in pancreatic cancer. Point mutations in K-ras oncogene occur in 85-95% of
pancreatic cancers (160) and inactivation of tumor-suppressor genes such as p16, p53 or
SMAD4 occurs in 98%, 75%, and 55% of pancreatic cancers, respectively (158).
Oncogenes and tumor-suppressor genes are also involved in cell cycle arrest and
apoptosis. BRCA2, TGF-, BAX mutations are found in pancreatic cancers. Several
tumor suppressor genes such as PENK, RARB, CDKN2A, CACNAIG, TIMP3, CDH1
AND MLH1 are often inactivated by CpG island hypermethylation (161).
The prognosis of pancreatic cancer is poor. If patients with pancreatic cancer are
diagnosed early, surgical resection is available and the 5-year survival rate in patients
who had surgery treatment is 21% (162).
However, for patients with unresectable
pancreatic cancer, the 2-year survival rate is 5%18%, and the 5-year survival rate is less
49
than 5% (163-165).
For advanced pancreatic cancer, chemotherapy or radiation
treatment, or a combination of two can be applied, however, pancreatic cancer is highly

resistant to chemotherapy and radiation treatment (166, 167).
5-Fluorouracil (5-FU) treatment was the standard treatment for pancreatic cancer
(166). Gemcitabine is currently used for the first choice of pancreatic cancer treatment.
Gemcitabine is an analog of deoxycytidine (Figure 3.1) and it blocks DNA elongation,
causing DNA fragmentation and cell death (168). Gemcitabine is a prodrug and it is
phosphorylated by deoxycytidine kinase to make gemcitabine monophosphate and then it
is converted to active, di- and triphosphate (169). This gemcitabine triphosphate is
incorporated into DNA chain and terminates DNA synthesis. Therefore, gemcitabine
shows cell killing effect at S-phase of cell cycle.
Reproducible clinical benefit of
gemcitabine was 23.8%, which is higher than 4.8% in 5-FU and the 1-year survival in
gemcitabine treated patients (18%) was also higher than in 5-FU treated patients (2%)
(170). Even though gemcitabine shows better effect than 5-FU, it is still marginally
effective. The combination therapy with other anticancer drugs including 5-FU, cisplatin,
oxaliplatin, docetaxel, irinotecan, capecitabine shows a better response than single agent
gemcitabine treatment (171).
However the survival rate is still poor and other
combinations of these cytotoxic reagents are needed to treat this disease.

Conceivably miRNA-based therapy of cancer may be achieved by inhibiting
miRNAs with antisense oligonucleotides in cancers that over express the miRNA. 2-Omethyl oligoribonucleotides (125, 126) or 2-O-methyoxyethyl (MOE) phosphorothioate
oligonucleotides (66) are effective, essentially irreversible, inhibitors of mature miRNA
function (125).
Various studies have demonstrated that transfection of chemically

50
modified antisense to cancer cell lines produces cell death in vitro (85, 87, 93, 109, 110,
172, 173). In vivo studies of anti-miRs also appear promising. Chemically modified
antisense to the liverspecific miR-122a produced sustained inhibition of the miRNA in
mouse liver in vivo for up to 23 days after a single injection (127). Injection of 2 MOE
antisense to miR-122 was effective at miR-122 degradation, target gene up regulation and
cholesterol lowering in normal mice (174).
We showed in chapter 2 that aberrant miRNA expression exists in pancreatic
adenocarcinoma (64). By profiling over 200 miRNA precursors in 28 pairs of tissues, we
identified a number of miRNAs with increased expression including miR-21, -221, -301,
-212 and others. There were some miRNAs with decreased expression however the
expression was predominately increased in the cancer. The fold-change in the expression
was quite high (e.g. 5- to 10-fold) in the tumors compared to the normal or adjacent
benign tissue.
Our results were corroborated by two additional studies (89, 175).
Szafranska, et al., used cDNA arrays to profile 281 miRNAs in 24 specimens of

pancreatic adenocarcinoma, chronic pancreatitis or normal pancreas (175). Bloomston, et
al., used miRNA arrays to profile the expression of 326 miRNAs in 107 specimens of
pancreatic adenocarcinoma or pancreatitis; to maintain cell heterogeneity, two mm tissue
cores of tumor or benign cells were isolated from the FFPE tissues (89). While some
inconsistencies were apparent between the three pancreatic adenocarcinoma studies (64,
89, 175), there was a fair amount of overlap. For example, both our study (64) and the
study by Bloomston, et al., (89) identified miR-221 as the top differentially expressed
miRNA in pancreatic adenocarcinoma.
51
In this study, we asked the question of whether inhibition of miRNAs that are
overexpressed in pancreatic cancer cell lines with antisense oligos results in cell death.
In addition, can miRNA inhibition sensitize the cells to the antiproliferative effects of
commonly used chemotherapeutic agents such as gemcitabine.
3.2.1 Cell lines and culture

The human pancreatic cancer cell lines, Panc-1, Miapaca-2, HS766T, SW1990,
PL45, MPanc-96 and CaPan-1 were purchased from American Type Tissue Collection
(Manassas, VA).
Panc-1, MiaPaca-2, HS766T were cultured in DMEM with L-
glutamine and 10% fetal bovine serum. SW1990 was cultured in L-15 medium with 10%
fetal bovine serum. Normal pancreatic ductal epithelial cell line, HPNE (human
pancreatic Nestin-expressing cell) (176), was a gift from Dr. Ouellette (University of
Nebraska Medical center, Omaha, NE). HPNE cells were cultured in Medium D (176).
SW1990 were grown at 37 C without CO2. All other cells were grown at 37 C in a
humidified atmosphere with 5% CO2.
3.2.2 miRNA profiling

The expression of 305 mature miRNAs were profiled in PANC-1, MiaPaCa-2,
PL45, MPANC-96, HS766T, CaPan-1, SW1990 and HPNE cells using the TaqMan
MicroRNA Assays (Applied Biosystems, Foster City, CA) as reported (60). miRNA
52
expression was related to the 18S rRNA internal control and data were calculated using
the comparative CT method.
3.2.3 Antisense oligonucleotides

Anti-miRTM miRNA inhibitor is purchased from Ambion (Austin, Texas). 2-Omethoxyethyl with phosphodiester backbone (2-O-MOE-PO) and 2-O-methoxyethyl
with phosphorothioate backbone (2-O-MOE-PS) antisense oligonucleotides were
provided by Isis Pharmaceuticals (Carlsbad, California).
Antisense oligonucleotide
(ASO) is the complementary sequence to the mature miRNA according to miRBase 9.0.
The sequences of the antisense oligonucleotides are shown in Table 3.1.
3.2.4 Antisense oligonucleotide transfection

The cells were plated one day before transfection and antisense were transfected
using lipofectamine 2000 and Opti-MEM medium (Invitrogen, Carlsbad, CA) following
the manufacturers protocol. Transfection efficiency was measured using a fluorescently
(DY547) labeled miR-16 oligo and the amount of fluorescence was determined by a
LS55 Luminescence Spectrometer (Perkin Elmer, Boston, MA). The knockdown effect
of the antisense oligonucleotide was measured by quantifying the target mature miRNA
using real-time PCR.
3.2.5 Gemcitabine treatment

Gemcitabine was purchased from Eli Lilly & Co (Indianapolis, IN). Cells were
plated in triplicate at 1200 cells/well in 96 well plates overnight. Gemcitabine was then
53
added at the desired concentrations and incubated for 48 and 96 h. After treatment, cells
were used for cell proliferation assay.
3.2.6 Cell proliferation assay

Cell proliferation assay was performed using the cell proliferation reagent WST-1
(Roche, Mannheim, Germany). Cells were seeded into 96-well plates at 1200 cells/well.
Cells were treated with gemcitabine and/or antisense and incubated for 48 or 96 h. After
incubation, 15 l of WST-1 was added to cell culture medium and incubated for 4 h.
Sample absorbance was analyzed using a microplate ELISA reader at 490 nm. All
experiments were performed at least in triplicate.
3.2.7 Combination assay

To determine the effects of ASO pretreatment on the gemcitabine potency,
HS766T cells were seeded in 100 mm Petri dishes at the initial density of 2x105 cells and
transfected with various concentrations of control oligonucleotide or ASO to miR-21, 212, -221 or -301 for 24 h.
Cells were then collected by trypsinization and then
transferred to a 96 well plate (3x103 cells/well). After incubating for 24 h to allow cells
to stabilize, gemcitabine was added in a 7 point serial dilution series, 3 replicate plate
columns per treatment. After 72 h of gemcitabine treatment, cell growth inhibition was
determined using WST-1 assay.
For the simultaneous combinatorial experiment, gemcitabine was given with
either ASO to miR-21, -212, -221, or -301 for 96 h. Each ASO was combined with
another ASO, (e.g. ASO to miR-21+ASO to miR-212) in HS766T cells for 96 h. Drugs
54
were combined at equitoxic ratios (i.e. doses were applied in combinations that would
have produced the same cytotoxic effect if the drugs were administered separately to
produce a 50% of growth inhibition, as determined by WST-1 assay). The cytotoxicities
of the two drug combinations were also determined by WST-1 assay using the same
procedure as used for single treatments. Each experiment was performed in triplicate.
3.2.8 Data analysis

ICx defined as the drug concentration required to reduce the absorbance to (100X) % of the control in each test was determined using an Emax model (Eq. 1).
[D]m + R (Eq. 1)
% Cell viability = (100 R ) 1 m
m
K d + [D]
where [D] is the drug concentration, Kd is the concentration of drug that produces a 50%
reduction in absorbance (i.e., IC50), m is a Hill-type coefficient, and R is the residual
unaffected fraction (the resistance fraction).
The nature of interactions between two drugs were determined by using the median effect
analysis (177). Median effect analysis permits the calculation of the combination index
(CI) at various levels of cell kill (Eq. 2). CI was calculated for a cell death range of 2080%, i.e., CI20-CI80.
CI x =
(D )A
(D X ) A
(D)B
(D X ) B
(D )A (D )B
(D X ) A (D X ) B
(Eq. 2)
where CIx is the CI for a fixed effect, x [fraction affected (fa) 100], for a combination of
drug A and drug B, (Dx)A is the concentration of drug A alone giving an effect x, (Dx)B
is the concentration of drug B alone giving an effect x, (D)A is the concentration of drug
55
A in combination A+B giving an effect x, (D)B is the concentration of drug B in

combination A+B giving an effect x, and is a parameter with value 0 when A and B are
mutually exclusive and 1 when A and B are mutually non-exclusive. A CIx less than 1
was categorized as synergistic and greater than 1 as antagonistic.
3.2.9 Statistical analysis

All data are represented as mean S.D. For comparison between two groups,
Students t test was used and p < 0.05 is considered as statistically significant.
3.3 RESULTS
3.3.1 miRNA expression in pancreas cell lines

We were initially interested in identifying pancreas cancer cell lines that had
increased expression of many of the top miRNAs that were increased in pancreas cancer
tissues (64). To determine this, the expression of over 300 mature miRNAs was profiled
in 7 pancreas cancer cell lines and in the normal HPNE cells. The results of this profiling
study are presented in Appendix Table B. Previous studies in tumors from pancreatic
cancer patients revealed that a number of miRNAs had increased expression in pancreatic
cancer (64, 89, 175). These include miR-212, -301 and -376a (64); miR-21 and -100 (64,
89); miR-221/-222 and -146 (64, 89, 175); and miR-210 and -223 (89, 175).
The
expression values of these top differentially expressed miRNAs in the 7 pancreas cancer
cell lines relative to the normal HPNE cells are shown in Table 3.2.
There is a
predominate increase in the expression of many of these miRNAs compared to HPNE.

56
From this analysis we selected five miRNAs for our antisense inhibition studies (miR-21,
-100, -221, -301 and -212).
These miRNAs were selected because they had
predominately increased expression in these cell lines and in the pancreas tumors (64).
Also miR-21 and miR-221 have been shown to be increased in a number of solid tumors
and antisense inhibition of both miRNAs reduced clonogenicity (178) or produced cell
death in vitro (87).
3.3.2 Antiproliferative effects of antisense oligonucleotides

The antiproliferative effects of three different chemical modifications to antisense
to miR-221 were studied. These include Anti-miR miRNA Inhibitor from Ambion,
Isis 2-O-MOE-PO (ISIS-PO) and Isis 2-O-MOE-PS (ISIS-PS). HS766T cells were
transfected with various amounts of antisense for 48 and 96 h. The result showed ISISPS is the most effective antisense (Figure 3.2). For the 96 h treatment of 50 nM, the
percentage survival was 76.316.0, 52.98.0, and 19.52.3 for Ambion, ISIS-PO, and
ISIS-PS, respectively. Therefore, ISIS-PS antisense to miR-21, -100, -212, -221, and 301 were selected for further studies.
The antiproliferative effects of inhibiting miR-21, -100, -212, -221, and -301 with
ASO was studied for 48 and 96 h of continuous exposure. HPNE (normal pancreatic
ductal epithelial cells) and four human pancreatic cancer cell lines (HS766T, SW1990,
Panc-1 and MiaPACA-2) were transfected with various concentrations of antisense using
lipofectamine 2000. All ASO showed a reduction in cell viability compared to the lipid
control in the human pancreatic cancer cell lines. Inhibition of cell viability with ASO
for 48 and 96 h produced IC50 in the low nM range (Table 3.3). The normal HPNE cells
57
were considerably less sensitive to the effects of some of the ASO (Figure 3.3A, Figure
3.3B and Table 3.3).
Inhibition of miR-21 with ASO produced very little
antiproliferative activity in HPNE cells including undetectable IC50 from triplicate

experiments (Table 3.3, Figure 3.3A, and Figure 3.3B). Inhibition of miRNAs with the
other 4 ASOs resulted in slightly greater antiproliferative activity in HPNE cells
compared to ASO-miR-21, although many of these ASO failed to produce a measurable
IC50 or IC70 in the replicate experiments (Table 3.3). Of the 5 ASOs, the IC50 of miR-301
in HPNE most closely approximated that of the pancreas cancer cell lines (Table 3.3).
These results suggest the possibility of selective cytotoxicity to pancreas cancer cells
compared to normal pancreas for some of the ASO.
The possibility exists that the HPNE cells were less sensitive to antisense because
transfection efficiency of the ASO was reduced compared to the pancreas cancer cell
lines. To test this, a DY547 labeled miR-16 oligo was transfected into both HPNE and
HS766T cells using lipofectamine 2000 and the amount of fluorescence was determined
by a spectrometer. The transfection efficiency of the miR-16 oligo was not reduced in
the HPNE cells and in fact was slightly greater than that of the HS766T cells (Figure
3.3C).
Hybridization of the antisense to the mature miRNA should reduce the amount of
freely available miRNA within the cells. To verify this, HS766T cells were transfected
with ASO-miR-221 and then the levels of mature miR-221 within the cells was
determined by quantitative RT-PCR. One hundred nM of ASO-miR-221 reduced the
amount of mature miR-221 by 70% (Figure 3.3D).
58
Experiments were conducted to evaluate any off target effects of the 2-O-MOEPS oligos. A 96 h continuous exposure of up to 100 nM of an irrelevant control oligo did
not reduce cell viability below 20% (Figure 3.3A and 3.4A). Two mismatch control
oligos to ASO-miR-221 were evaluated; one with 3 nts mismatched and another with 6
nts (Table 3.1). There was a trend of reduced cytotoxicity with increasing number of
mismatched nts (Figure 3.4B).
3.3.3 Schedule-dependent combinatorial effects of antisense with gemcitabine

Gemcitabine is one of the agents used to treat pancreatic adenocarcinoma,
however it is only marginally effective.
It is possible that miRNA inhibition may
sensitize cancer cells to the effects of commonly-used chemotherapeutic agents such as

gemcitabine. This effect was demonstrated for the combination of ASO-miR-21 and
gemcitabine in cholangioma cell lines (110). At first, we examined whether addition of
gemcitabine to ASO-primed HS766T cells would result in a lower IC50 for gemcitabine.
For this experiment, the cells were pre-treated with antisense for 24 h followed by
gemcitabine for an additional 72 h. We found that sequential addition of gemcitabine to
ASO-miR-21 primed HS766T cells resulted in a reduction in the gemcitabine IC50. The
gemcitabine IC50 was reduced from 5.9 nM (control oligo 50 nM) to 1.2 nM (30 nM of
ASO-miR-21) to 1.0 nM (70 nM of ASO-miR-21), respectively (Figure 3.5A).
In
addition, antiproliferative effects of gemcitabine were also enhanced by pretreatment of

ASO-miR-221 (20 nM) and ASO-miR-301 (50 and 70 nM). Effects of low concentration
of gemcitabine ranging from 0.1 to 10 nM were not enhanced by ASO pre-treatment
(data not shown).
However, at high effect levels (50, 200, and 500 nM), the
59
antiproliferative effects of gemcitabine were greatly increased in ASO-miR-221 and -301

primed HS766T cells compared to control oligo (Figure 3.5B).
Next, we analyzed the interaction of gemcitabine and ASO on cell survival rate
using the median effect/combination index method after exposing two agents
simultaneously for 96 h. The CIx values calculated for 0.2 fa 0.8 (i.e. fraction
affected between 20% and 80%) are shown in Figure 3.5C. All combinations were
examined at a fixed molar ratio based on approximate equitoxic concentrations at the
level of 50% growth inhibition of each agent, i.e. IC50 of drug A: IC50 of drug B. For
each ASO, the IC50 values are shown in Table 3.3. The IC50 value of gemcitabine alone
in HS766T cells was 3.2 0.3 nM for a 96 h exposure. CIx values for the combination
ASO-miR-21+gemcitabine (1:6 molar ratio), ASO-miR-212+gemcitabine (1:2 molar
ratio), ASO-miR-221+gemcitabine (1:2 molar ratio), and ASO-miR-301+gemcitabine
(1:4 molar ratio) varied with fa. CIx values of the above 4 cases were decreased from 2.9,
2.7, 1.8 and 1.3 at fa=0.2 to 0.8, 0.96, 0.68 and 1.2 at fa=0.8, respectively. In the
clinically relevant range of fa 0.5, ASO-miR-221+gemcitabine combination was
considered
to
be
synergistic.
The
ASO-miR-21+gemcitabine,
ASO-miR-
212+gemcitabine and ASO-miR-301+gemcitabine appeared to be antagonistic.
The
antagonistic effects of ASO-miR-21+gemcitabine and ASO-miR-212+gemcitabine were

more evident at lower drug concentration, with CI= 2.9 and 2.7 at fa=0.2, respectively.
3.3.4 Evaluation of synergism in ASO combination

To extend our observations, we evaluated the antiproliferative effects of various
ASO-miR-21, -212, -221, or -301 combined with each other in HS766T cells for 96 h.
60
All combinations were also examined at fixed molar ratio based on approximate
equitoxic concentrations at the level of 50% growth inhibition of each agent.
For
ASO+ASO simultaneous combinations, we tested six different combinatorial effects,

which are ASO-miR-21+ASO-miR-212 (1:0.3 molar ratio), ASO-miR-21+ASO-miR-221
(1:0.3 molar ratio), ASO-miR-21+ASO-miR-301 (1:0.7 molar ratio), ASO-miR212+ASO-miR-221 (1:1 molar ratio), ASO-miR-212+ASO-miR-301 (1:2 molar ratio),
and ASO-miR-221+ASO-miR-301 (1:2 molar ratio), in HS766T cells. Among them,
ASO-miR-212+ASO-miR-301 showed the greatest synergism with CI value of less than
1.0 (0.55-0.99) for the whole fa range (Figure 3.6), indicating a greater advantage
compared to the other combinations. ASO-miR-221+ASO-miR-301 combination also
showed synergism at fa=0.2, but was found to be antagonistic with CI values ranging
from 0.99 to 2.0 at fa=0.5 and 0.8, respectively (Figure 3.6). When ASO-miR-21 was
combined with ASO-miR-212 or ASO-miR-221, slight synergistic effects were shown:
the CI80 was 0.80 for the ASO-miR-21+ASO-miR-212 and CI20 was 0.68 for the ASOmiR-21+ASOmiR-221 (data not shown). However, the CI20 and CI80 were 1.78 and 1.79
for
ASOmiR-21+ASO-miR-212
and
ASO-miR-21+ASO-miR-221,
respectively,
indicating antagonistic interactions. ASO-miR-21 was also treated with ASO-miR-301

and showed antagonistic interactions (CI values > 1) for the whole fa range. On the other
hand, only antagonistic interactions were induced by ASO-miR-212+ASO-miR-221 with
CI values increasing from 1.6 to 2.5 by escalating concentrations of each ASO (data not
shown).
61
3.4 DISCUSSION
The results of our current study demonstrate that inhibition of 5 miRNAs that are
over expressed in pancreas cancer produced cell death in vitro. Low nM concentrations
of ASO reduced cell proliferation in pancreatic cancer cell lines. ASO inhibition of
miRNA in the normal HPNE cells had less pronounced effects on cell proliferation
compared to the pancreas cancer cell lines (Figures 3.3A and 3.3B and Table 3.3).
Certain 2-O-MOE-PS ASOs that were not complementary to any known human gene
were observed to substantially effect cell proliferation at concentrations of 100-300 nM in
vitro (179). Phosphorothioates in general have been shown to produce toxicity (reviewed
in (129)). Therefore it is possible that a portion of the cytotoxicity from the 2-O-MOEPS was due to off target effects. Exposing cancer cell lines to an irrelevant control 2-OMOE-PS oligo produced modest effects on cell proliferation (Figures 3.3A and Figure
3.4A). Likewise, altering the sequence of ASO-miR-221 reduced its antiproliferative
activity (Figure 3.4B).
Standard palliative gemcitabine treatment showed only a 6 month median survival.
Although many trials have been attempted to improve upon the overall survival of
gemcitabine
based
combination
chemotherapy,
only
the
gemcitabine/Erlotinib
combination showed a slight increase in the overall survival, but survival times
associated with combinations of gemcitabine/fluorouracil, gemcitabine/irinotecan,
gemcitabine/cisplatin, and gemcitabine/oxaliplatin appear to be either equivalent or only
marginally superior to those associated with single-agent gemcitabine (180). A phase III
study showed that a 4-drug PEFG (cisplatin, epirubicin, 5-fluorouracil (5-FU),
62
gemcitabine) regimen obtained a response rate of 38-51% and a 1-year overall survival of
38.5-39%, without compromising quality of life, which was significantly better than
single-agent gemcitabine (180).
However, there are still no guidelines or
recommendations for selecting treatment for pancreatic cancer patients, and both
acquired drug resistance and considerable systemic toxicities are major reasons for the
limited advances made in cancer chemotherapy. From this point of view, our data
showing the antiproliferative effects in various pancreatic tumor cells, reduced sensitivity
of antisense toward normal HPNE cells and enhancement of gemcitabine antiproliferative
effects with ASO-miR-21, -221 and -301 and a synergistic effect between gemcitabine
and ASO-miR-221 (Figure 3.5) suggests that ASO can be used as future gemcitabinebased combination regimen. Moreover, our results showing the greatest synergism of the
ASOmiR-212+ASO-miR-301 combination (Figure 3.6) also imply, at least in part, that
we can expect greater effects than gemcitabine-based combinations.
Overall,
experiments described herein strongly support the potential for the miRNA-based
targeted therapy of pancreatic cancer using antisense oligonucleotide and the possible
target molecules and more detail mechanisms that are responsible for anti-proliferation
and sensitization effect should be further investigated.
Supported by an NIH grant CA107435 to T.D.S. and a grand from the National
Pancreas Foundation. We thank Dr. Caifu Chen at Applied Biosystems for his assistance
with the TaqMan looped primer assays and Christine Esau at ISIS Pharmaceuticals for
63
her assistance with the Antisense oligonucleotides. We also thank Jong-Kook Park with
experiments assistance.
64
Figure 3.1. Structures of Gemcitabine and Deoxycytidine.
65
ASO221(48hr)
120
100
Survival(%)
80
Ambion-221
ISIS(PO)-221
ISIS(PS)-221
60
40
20
0
lipo 20
50 250 500 lipo 20
50 250 500 lipo 20
50 250 500
ASO221(96hr)
140
Survival(%)
120
100
Ambion-221(96)
ISIS(PO)-221-96
ISIS(PS)-221-96
80
60
40
20
0
lipo 20
50 250 500 lipo 20
50 250 500 lipo 20
50 250 500
Figure 3.2. Comparison of three different antisense oligonucleotides.

WST-1 assay in pancreatic cancer (HS766T) cells show the relative cytotoxicity of the
antisense to miR-221. Ambion anti-miR was compared to Isis 2 MOE-PO and Isis 2
MOE-PS. Cells were exposed continuously for 48 h (top) or 96 h (bottom). Lipo, lipid
only control. Cells were transfected using lipofectamine 2000.
66
Figure 3.3. The effect of antisense oligonucleotide and control oligonucleotide in

pancreatic cell lines.
(A) Representative dose-response curve of ASO-miR-21 and ASO-miR-221 and control
oligonucleotide in HS766T and HPNE. Cells were transfected with oligonucleotides and
incubated continuously for 96 h. Filled symbols are HS766T, open symbols are HPNE,
circles are ASO-miR-21, triangles are ASO-miR-221, and squares are control
oligonucleotide. (B) Antiproliferative effects of ASO-miR-21 and ASO-miR-221 (50 nM,
96 h) in 5 pancreatic cell lines. (C) Cellular uptake of fluorescently labeled miR-16
oligonucleotide in HS766T and HPNE cells. (D) The knockdown effect of ASO-miR221 in HS766T cells. The expression levels of the target microRNA were assessed by
real-time RT-PCR 24 hours after transfection. Data presented in (B) and (D) are the
mean of 3 independent experiments (* P < 0.05; ** P < 0.01).
67
68
Figure 3.4. Effect of control oligonucleotides on cell proliferation.

(A) Transfection of control oligonucleotide in HS766T cells for 96 h; data are compared
to lipofectamine control. Mean data from triplicate transfections. (B) Comparison of
wild type ASO-miR-221, 3 nt and 6 nt mismatch control oligonucleotides on the
proliferation of HS766T cells; 96 h exposure. Mean data of 3 independent experiments.
69
Figure 3.5. ASO pretreatment sensitized the antiproliferative effects of gemcitabine.

Antisense to miRNAs are transfected in HS766T cells 24 h before gemcitabine treatment
and antiproliferative effects of gemcitabine are measured after 72 h treatment by WST-1
assay. ASO sensitized the gemcitabine effect showing that dose-response curve is shifted
to the left. (A) Representative dose-response curve of gemcitabine after ASO-miR-21(30
nM , 70 nM ,) and control oligonucleotide pretreatment (50 nM, ). (B) ASO-miR221 and ASO-miR-301 sensitize the gemcitabine effect at the high concentrations. (C)
Combination index (CIx) versus fraction affected (fa) plots in HS766T cells for
ASOmiR-21+gemcitabine (
), ASO-miR-212+gemcitabine (

), ASO-miR221+gemcitabine (
), and ASO-miR-301+gemcitabine ().
70
71
Figure 3.6. Combination index (CIx) versus fraction affected (fa) plots in HS766T
cells for ASO-miR-212 + ASO-miR-301 (

) and ASO-miR-221 + ASO-miR-301
(
). CI < 1 and CI > 1 indicate synergism and antagonism, respectively. CIx was
calculated using Emax model parameters obtained from the WST-1 assay data.
72
Isis number
miRNA
Sequence
(5 to 3)
327917
miR-21
TCAACATCAGTCTGATAAGCTA
327957
miR-100
CACAAGTTCGGATCTACGGGTT
327891
miR-212
GGCCGTGACTGGAGACTGTTA
346908
miR-301
ACGTGGATTTTCCTCTATGAT
327919
miR-221
GAAACCCAGCAGACAATGTAGCT
394088
394091
386666
miR-221
3 nt mismatch
miR-221
6 nt mismatch
GAATCCCAGCAGAGAATGTTGCT
GAATCACACCAGAGAAAGTTGCT
Irrelevant control
ACATACTCCTTTCTCAGAGTCCA
Table 3.1. Sequence and chemical modifications to antisense oligonucleotides.

Each of the antisense oligonucleotides is modified with uniform MOE
w/phosphorothioate backbone [AGTmC]. Each has a 5 end cap of hydrogen and 3 end
cap of hydroxyl.
73
miRNA
miR-21
miR-221
miR-100
miR-212
miR-301
miR-376a
miR-155
miR-210
miR-223
miR-146
miR-222
miR-24
PANC-1
MIAPACA-2
PL45
MPANC-96
HS766T
CaPan-1
Sw1990
2.9
0.6
1.3
3.3
21.2
3.0
3.0
1.8
1.2
59.1
0.6
1.1
2.8
2.5
0.0
2.3
52.9
0.0
0.1
1.2
1.8
6.6
1.9
1.1
3.1
1.2
0.2
2.1
11.6
0.0
2.0
1.2
0.0
2.4
1.4
0.8
5.2
2.0
0.2
1.5
15.3
0.0
1.9
4.1
3.3
35.7
0.9
1.6
9.1
1.9
0.8
3.0
86.8
0.0
14.7
0.9
0.2
7.0
1.7
0.9
7.7
0.4
0.0
0.6
5.1
0.0
0.5
4.1
1.3
99.9
0.4
1.8
2.2
1.7
0.3
1.0
7.7
0.0
1.5
1.9
0.7
3.7
1.3
2.2
74
Red > 2-fold increase; Blue > 2-fold decrease
Table 3.2. miRNA expression, pancreas cancer cell lines normalized to normal pancreas cell (HPNE).
Table 3.3. Antiproliferative activity parameters of ASO against miR-21, -100, -212, 221 and -301 in human pancreatic cell lines.
The dose-response curves of these agents were analyzed using the Emax model (Eq. 1).
75
Cell lines
Treatment
duration
HS766T
48 h
96 h
SW1990
48 h
96 h
MiaPACA-2
48 h
76
96 h
Panc-1
48 h
96 h
HPNE
48 h
96 h
Parameter
IC50a
IC70 a
c
R
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
IC50 a
IC70 a
Rc
Anti-miR-21
48.34.4
98.410.9
13.87.6
28.48.9
44.012.8
0
NDb
NDb
74.014.4
21.47.9
42.68.7
13.87.1
22.318.6
43.621.3
3.56.1
15.07.8
24.88.9
6.18.9
66.132.8
NDb
39.37.7
37.93.5
NDb
34.61.4
NDb
NDb
95.611.7
NDb
NDb
73.226.3
Anti-miR-100
57.316.7
136.768.9
8.915.5
19.23.7
31.43.7
2.84.9
NDb
NDb
62.818.7
16.212.1
36.617.8
1.11.9
21.211.5
48.013.8
3.35.6
9.34.3
17.48.1
5.55.2
NDb
NDb
63.06.2
50.510.3
NDb
37.74.5
NDb
NDb
86.818.0
87.117.6
214NDe
62.025.3
Anti-miR-212
58.722.0
113.929.1
0
12.33.9
22.44.3
0
NDb
NDb
52.76.6
18.86.5
37.412.7
11.16.4
71.643.7
191.8162.9
0
12.24.1
16.77.1
<1.0
NDb
NDb
66.213.8
55.325.6
69.914.7
16.510.8
121.8 NDe
217.1NDe
63.67.90
66.310.5
120.910.8
34.05.15
Anti-miR-221
32.42.3
99.429.5
18.315.9
12.14.3
21.05.2
3.66.2
NDb
NDb
57.91.3
10.15.7
16.19.6
15.15.7
24.916.1
51.512.5
<1.0
2.51.7
3.62.6
10.39.8
64.324.4
NDb
39.46.9
10.12.9
NDb
31.92.3
1,4791,842
479.7 NDe
70.78.98
44.217.6
88.67.60
31.06.33
Anti-miR-301
57.014.0
106.630.2
0
24.01.9
39.61.2
0
43.312.4
138.159.3
22.61.3
14.94.0
26.84.9
2.72.5
41.99.6
62.817.2
8.23.4
20.41.6
26.610.7
<1.0
89.418.9
NDb
47.75.2
49.921.8
81.636.9
23.411.5
155.849.9
285.6 NDe
55.56.38
56.510.5
87.16.0
23.91.99
CHAPTER 4
ANTISENSE INHIBITION OF microRNA ARRESTS CELL CYCLE, INDUCES

APOPTOSIS AND IDENTIFIES THE TARGET MOLECULES IN PANCREATIC
CANCER
4.1 INTRODUCTION
A growing body of data implicates altered miRNA expression in cancer. The

initial study to establish a link between miRNA and cancer was from the Croce
laboratory.
Numerous profiling studies have shown that miRNAs are differentially
expressed in a number of human cancers including thyroid (90, 181), breast (99, 182),
lung (98), pancreatic adenocarcinoma (64, 89, 175), glioblastoma (87, 100),
hepatocellular (81, 82), prostate (183), colorectal (184, 185), leukemia (75, 153) and
lymphoma (101). A polycistron encoding miRNAs miR-17, -18, -19a, -19b-1 and -92-1 is
amplified in human B-cell lymphomas and forced expression of the polycistron along
with c-myc was tumorigenic, suggesting that this group of miRNAs may function as
oncogenes (94). Clear evidence exists that miRNAs are involved in cancer.
What is less clear is the mechanism(s) by which altered miRNA expression
contributes to the development or maintenance of cancer. To understand the function and
77
mechanistic pathway of miRNA, the miRNAs target gene must be discovered and
properly validated. The miRNAs target gene may be identified from a number of freely
available data bases. However, these programs have a high percentage of false positive
results. An issue with these prediction algorithms is that since the miRNA does not need
to bind with 100% complimentarity (except the 5 seed), several hundreds of potential
targets may be predicted for a given miRNA. Using miR-21 as an example, TargetScan,
miRanda and PicTar predict 192, 1000 and 175 targets, respectively. Different prediction
programs follow their own criteria so each program predicts different targets. Therefore,
it is important to narrow down the list of target candidates using several programs and to
properly validate the predicted target.
The target site accessibility can be considered as an important criterion in target
prediction because mRNA secondary structure may inhibit the target gene and miRNA
binding. Accessibility is estimated by the free energy of the flanking 70 nts at the 3 and
5 ends of the target site in the 3 UTR (70). Once a target candidate looks promising, the
miRNA and mRNA binding should be validated using a reporter assay. The 3 UTR
sequence of the target candidate gene is cloned downstream of a reporter gene such as
luciferase or green fluorescent protein (GFP). This cloned plasmid is transfected with
miRNA mimic or negative control into cells and the expression of reporter gene is
measured in 24-48 h after transfection. For controls, a mutated seed sequence, reversed
sequence of 3 UTR, or vector only may be used. Another way to check miRNA/mRNA
binding is to precipitate Ago complex and compare the sequences of the 3 UTR and
miRNA to identify functional miRNA targets in vivo (186).
78
After the binding assay, the protein level of the target gene should be validated by
western blot to see if gene knockdown affects the actual protein function. If an antibody
for target protein is not available, other validation such as ligand binding assay should be
performed (187). Target protein expression may be validated using gain of function or
loss of function studies. If endogenous miRNA is highly expressed, miRNA expression
can be blocked using antisense oligonucleotides resulting in an increase in the target
protein level. However, if the target gene expression is high and the miRNA expression
is not high enough to see a change of protein expression, a miRNA mimic may be added
and target protein should decrease. In addition, genes down stream of the miRNAs
target gene can be checked to see biological function of target gene. For example, the
Patel laboratory identified PTEN as a target gene of miR-21 (110) and they showed that
inhibition of miR-21 in human hepatocellular cancer increased PTEN level and decreased
tumor cell proliferation, migration, and invasion (86). They also demonstrated that focal
adhesion kinase phosphorylation and matrix metalloproteases 2 and 9, which are
downstream of PTEN, are involved in cell migration and invasion (86).
In our previous study, antisense oligonucleotides to miRNA-21, -212, -221 and
301 showed anti-proliferation effect at low nM concentration and sensitized the effect of
gemcitabine in a schedule-dependent way. Here, we investigated the effects of antisense
oligonucleotides on the cell cycle and apoptosis. We validated that PTEN and RECK are
target molecules of miR-21 and that p27(kip1) is the target of miR-221 in pancreatic
cancer. We also used a reporter assay to identify new target genes for miR-212 which is
FOXO3a.
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4.2.1 Cell lines and culture

The human pancreatic cancer cell line HS766T was purchased from American
Type Tissue Collection (Manassas, VA). HS766T was cultured in DMEM with Lglutamine and 10% fetal bovine serum, and it was grown at 37C in a humidified
atmosphere with 5% CO2.
4.2.2 Antisense Oligonucleotides and Pre-miR miRNA Precursor

2-O-methoxyethyl with phosphorothioate backbone (2-O-MOE-PS) antisense
oligonucleotides was provided by Isis Pharmaceuticals (Carlsbad, California). Antisense
oligonucleotides sequences are presented in Table 3.1. Pre-miR miRNA Precursor
Molecule was purchased from Ambion (Austin, Texas).
4.2.3 Antisense oligonucleotide and Pre-miR miRNA Precursor transfection

HS766T cells (2x105 cells in 100 mm dishes) were plated one day before
transfection and antisense or pre-miR were transfected using lipofectamine 2000 and
Opti-MEM medium (Invitrogen, Carlsbad, CA) following the manufacturers protocol.
Cells were incubated for 24 h, 48 h or 72 h depending on experiments.
4.2.4 Cell cycle analysis

To determine cell cycle distribution, cells were collected after trypsinization into a
single cell suspension, fixed with cold ethanol at 4 C for 1 h prior to being stored at -20
80
C until required for analysis. Upon analysis, fixed cells were washed and resuspended
in 1 ml of PBS containing 50 g/ml of RNase A and 50 g/ml of ethidium bromide.
After incubating for 20 min at 37 C, cells were analyzed for DNA content by flow
cytometry (FACS Caliber, Becton Dickinson Immunocytometry Systems, San Jose, CA).
For each sample, 10,000 events were acquired and cell cycle distributions were
determined using cell cycle analysis software (Modfit, Verity, Topsham, ME).
4.2.5 Apoptosis analysis

Annexin VFITC/propidium iodide (PI) staining was done to identify the
induction of apoptosis in HS766T cells treated with ASO. HS766T cells were plated in
100 mm Petri dishes at 2x105 cells per dish and cultured overnight. Twenty or 48 h after
ASO treatment, cells were collected and washed with PBS. Cells were stained with
Alexa Fluor 488 annexin V and PI using Vybrant Apoptosis Assay Kit #2 (Molecular
Probes, Eugene, OR) according to the manufacturer's protocol. Cells were analyzed
using flow cytometry (FACS Caliber, Becton Dickinson) and CellQuest software (Becton
Dickinson).
Gating was selected using untreated cells to separate the majority of
doublenegative cells from minor populations of PI or Annexin V-FITCpositive cells.

Cells that were FITC/PI were considered viable, FITC+/PI cells were considered
early apoptotic, and FITC+/PI+ cells were considered nonviable.
4.2.6 Real-time PCR

Total RNA was isolated from cells using Trizol (Invitrogen, Carslbad, CA) per
the manufacturers instructions. RNA was treated with DNase I and 1 g of RNA was
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reverse transcribed using random primers.

The expression of FOXO3a was measured using a SYBR green real-time
quantitative PCR assay and normalized to 18S rRNA. Relative gene expression was
calculated using 2-(CTFOXO3a CT18S rRNA) . PCR primers for FOXO3a were followings;
sense
primer,
5-TTGCGTGCCCTACTTCAA-3
and
antisense
primer,
5-
GCATGAATCGACTATGCAGTG-3.
4.2.7 Plasmid constructs

The 3 UTR of the human FOXO3a (FKHRL1) was amplified using the following
sense (5-ATGCCTCGAGATCTGCTCTCAGCAAGTGGA-3) and antisense primer
(5-ATTAGCGGCCGCTGGAGAGCTGAGCTGATTTCT-3)
containing
enzyme
restriction site (XhoI and NotI) at the both ends. Amplified PCR product was cloned at
downstream of the Renilla luciferase stop codon in psiCHECKTM-2 vector (Promega,
Madison, WI). Renilla luciferase is a primary reporter gene in psiCHECKTM-2 vector
and this vector also contains a secondary Firefly luciferase reporter cassette, which was
used to normalize the Renilla luciferase signal. Constructed plasmid was verified by
DNA sequencing.
4.2.8 Luciferase assay

HS766T cells (0.5x105 cells) were plated in 24 well plates one day before
transfection. Plasmid construct was transfected with pre-miR-negative control or premiR-212 using lipofectamine 2000 and Opti-MEM medium (Invitrogen, Carlsbad, CA).
Cells were harvested 24h later and Renilla and Firefly luciferase activity were measured
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using the Dual Luciferase Assay (Promega, Madison, WI) according to the companys
protocol.
4.2.9 Western blotting

HS766T cells (2x105 in 100 mm dish) were treated with an IC50 dose of ASO to
miR-21, -221 and -212 for 24, 48, and 72 h or with a 30 nM or 60 nM of pre-miR-212.
Cells were washed in ice-cold PBS and lysed in RIPA buffer containing protease
inhibitor. Protein concentration was measured using the BCATM protein assay kit (Pierce,
Rockford, IL). Thirty g of total protein extract was separated on a 10% SDS-PAGE gel.
Blotting was performed with the following antibodies: PTEN (Cell signaling, Danvers,
MA), RECK (Santa Cruz Biotechnology, Santa Cruz, CA), p27 (kip1) (Abcam,
Cambridge, MA) and FOXO3a (Upstate, Temecula, CA). -actin (Cell signaling, Danver,
MA) was used as a loading control. Secondary HRP antibody was detected using ECLTM
Western Blotting Analysis System (Amersham Biosciences, Piscataway, NJ).
4.3. RESULTS
4.3.1 Effects of miRNA inhibition on the cell cycle

The effects of inhibiting miR-21, -100, -221, -301 and -212 on the cell cycle were
studied in HS766T cells. A 48 h exposure of 20 nM of control oligo produced 57.1% of
G1, 12.4% of G2, 30.5% of S phase and a slight increase in the sub-G1 phase compared
to the lipid control (Figure 4.1A), however it produced no net effect on the cell cycle. An
increase in the sub-G1 fraction suggests the presence of apoptosis. Even at a higher
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concentration of 40 nM (48 h exposure), the control oligo showed almost the same cell
cycle distribution as the lipid control (data not shown). miR-21 inhibition resulted in
62.2% of G1, 15.9% of G2, and 21.9% of S phase at IC50 (48 h exposure) and the G1
phase was increased to 65.2% at the IC70 (48 h exposure). This demonstrates that miR21 inhibition produced a G1 phase arrest as the amount of cells in the G1 phase increased
by about 10% and the amount of cells in the S phase decreased by about 10% (Figure
4.1B). Inhibition of miR-301 increased the sub-G1 fraction to 42.5% at an IC50 dose
(Figure 4.1C). Inhibition of miR-212 produced a 19.6% of G2 at IC50 and the G2 phase
was increased to 30.3% at an IC70 dose (Figure 4.1D). Thus ASO-miR-212 produced a
G2 phase arrest. Conversely, inhibiting miR-221 did not show any noticeable changes in
the cell cycle at the IC50 by 48 h (data not shown) and or an IC70 treatment (data not
shown). Inhibition of miR-100 also didnt show any prominent changes at IC50 (48 h
exposure, data not shown).
4.3.2 Effect of miRNA inhibition on apoptosis

Since the results of the cell cycle analysis demonstrated that inhibition of some
miRNAs increased the sub-G1 fraction and antisense to miR-21 has been shown to
induce apoptosis (87), we wanted to examine the effects of miR-21, -221, -212 and -301
inhibition on apoptosis. This was done to determine whether the antiproliferative effects
of miRNA inhibition were due to apoptosis induction. Phosphatidylserine externalization
is a parameter of ongoing apoptosis, which can be measured by Annexin V staining. The
results of Annexin V staining showed that early apoptotic cells (Annexin V positive cells)
were increased in a time-dependent manner in HS766T cells (Figure 4.2). Only 4.91.2
84
% and 11.42.9% of cells were annexin V positive after 24 and 48 h incubation of 50 nM

of control oligo, respectively. However, HS766T cells transfected with ASO-miR-21, 212, -221 or -301 induced 19.9%, 6.0%, 10.2% and 15.8% of annexin V positive cells
after 24 h, respectively. Furthermore, the transfection of each ASO for 48 h dramatically
increased the apoptotic cells compared to control oligo. The percentage of cells staining
positive with Annexin V was 58.2%, 28.8%, 36.6%, and 53.1% for ASO-miR-21, -212, 221, and -301, respectively (Figure 4.2). Relatively low concentrations (25 nM) of ASOmiR-21 and -301 also showed 21.01.5 and 25.41.3% of Annexin V positive cells after
48 h incubation (data not shown). In addition, these data showed that ASO-miR-21 and 301 induce a relatively higher amount of apoptotic cells compared to ASO-miR-212 and 221.
4.3.3 PTEN, RECK and p27(kip1) validation as target of miR-21 and miR-221
miRNAs exert their activity by binding to conserved sequence within the 3 UTR
of protein coding mRNAs. miRNA target discovery and validation is an active area of
research and the targets to few mammalian miRNAs have been discovered. Some of the
mRNA targets to miR-21 and miR-221 have been recently discovered. miR-21 has been
shown to target the tumor suppressor PTEN (110). Three groups working independently
demonstrated that miR-221 targets the tumor suppressor p27(kip1) (178, 180, 188). The
tumor suppressor RECK (reversion-inducing cysteine-rich protein with Kazal motifs) is
predicted to be a target of miR-21 (107). In order to validate that the ASO to miR-21 and
miR-221 is working through a miRNA effect (i.e. at the level of translation), we
performed Western blots to detect these tumor suppressors in HS766T cells treated with
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control oligo or antisense oligonucleotide for various times. The amount of p27(kip1)
protein increased over the 72 h period of ASO-miR-221 treatment (Figure 4.4B). This
demonstrates that as miR-221 is inhibited by antisense, less miRNA is available to
negatively regulate the p27(kip1) mRNA. ASO-miR-21 increased both PTEN and RECK
levels at 24, 48 and 72 h of exposure (Figure 4.4A).
4.3.4 FOXO3a as a new target of miR-212

FOXO3a is one of the mammalian forkhead members of the class O (FOXO)
transcription factors and is involved in cell cycle arrest and apoptosis (reviewed in (189)).
Since ASO-miR-212 showed cell cycle arrest at G2/M phase and also induced apoptosis,
we wanted to identify a possible target gene of miR-212 that explains the mechanism of
cell cycle arrest and apoptosis. First, target candidates of miR-212 were investigated
using several target prediction programs such as TargetScan, miRanda and PicTar.
Among numerous predicted target genes, FOXO3a was one of promising candidates
because all three programs predicted FOXO3a as a target of miR-212 and it is a gene that
regulates both cell cycle and apoptosis. FOXO3a contains a putative miR-212 binding
site in its 3 UTR. The potential base pairing between FOXO3a and miR-212 is shown
in Figure 4.4A. The binding site is highly conserved in several species (Figure 4.4A).
It was reported that the secondary structure of the 3 UTR of the target gene may
prevent miRNA from binding and that miRNA accessibility may be a critical
characteristic of target binding (70). To further confirm that FOXO3a may be a target
gene of miR-212, we calculated the free energy of the ~ 70 nt flanking the 5 and 3
86
regions of the predicted binding site using mFold (190). The values are -7.8 and -12.8
kcal/mol, respectively, showing a favorable binding.
The expression of mRNA level of FOXO3a was quantified using a real-time PCR
assay after antisense oligonucleotide (ASO) transfection. HS766T cells were treated with
either control oligonucleotide (20 nM) or ASO-miR-212 (20 nM) for 24 h and 48 h. The
data demonstrated that the mRNA level was significantly increased in the cells that were
treated with ASO-miR-212 for 48 h compared to untreated or control oligonucleotide
treated cells (Figure 4.4 B).
To verify whether miR-212 interacts with the 3 UTR of the FOXO3a, FOXO3a
3 UTR was cloned downstream of the Renilla luciferase translational stop codon. This
plasmid construct was transfected into HS766T cells with either pre-miR-negative control
or pre-miR-212 oligonucleotides. Cells were incubated for 24 h and Renilla luciferase
activity was normalized with Firefly luciferase activity. As shown in Figure 4.5A, the
luciferase activity in cells treated with pre-miR-212 was significantly reduced by
approximately 50% compared to the cells with pre-miR-negative treatment, indicating
that miR-212 binds to the 3 UTR of FOXO3a.
miRNA binds to the 3 UTR of the target gene and represses the target gene
translation (11). To determine whether miR-212 regulates FOXO3a expression through
translational repression, we performed western blotting with anti-FOXO3a antibody
(Figure 4.5 B and 4.5 C). We treated the cells with ASO-mir-212 in various time points
and protein is up-regulated at 48 h and 72 h (Figure 4.5 B). In addition, cells were treated
with pre-miR-negative and pre-miR-212 oligonucleotide at 30 nM and 60 nM and protein
87
level of FOXO3a was reduced (Figure 4.5 C).
The results that we showed here
demonstrate that FOXO3a is a target gene of miR-212.
4.4 DISCUSSION
Consistent with previous reports, antisense to miR-21 resulted in a concentration

dependent G1 phase arrest and induction of apoptosis in the pancreatic cancer cell line
HS766T (Figure 4.1 and 4.2). miR-21 is highly expressed in human glioblastoma tumor
tissues and knockdown of miR-21 induced activation of caspases (87).
Apoptosis
induced by ASO-miR-21 was due to downregulation of Bcl-2 in the MCF-7 breast cancer
cell line (85). In addition, the tumor suppressor PTEN that coordinates G1 cell cycle
arrest is a validated target of miR-21 (110). In HS766T pancreatic tumor cells, treatment
of ASO-miR-21 also increased PTEN protein expression (Figure 4.3A) and induced a G1
cell cycle arrest (Figures 4.1B). We also observed that miR-212, -221 and -301 regulates
the cell cycle and/or apoptosis in pancreatic tumor cells.
ASO-miR-21 also increased RECK protein levels in HS766T cells over the 72
hours of exposure.
RECK is a membrane bound protein that inhibits matrix
metalloproteinases and angiogenesis (191). Increased expression of miR-21 in pancreatic

adenocarcinoma (64, 89), may likely suppress RECK protein levels. In fact, pancreatic
adenocarcinomas with positive RECK staining were less invasive compared to RECK
negative tumors and patients who had tumors with high RECK expression showed
significantly better prognosis than those with RECK-negative tumors (192).
88
Previous studies demonstrated that p27(kip1), which plays a key role in the
growth arrest at the G1/S transition, is one of the targets of miRNA-221 (178, 180, 188).
Visone, et al., showed that treatment of TPC-1 cells with miRNA-221 mimetic increased
the transition from the G1 to S phase and reduced p27(kip1) protein (180). Also, ectopic
expression of miR-221 and -222 enhanced the progression to the S phase of cell cycle,
therefore, increasing the growth of LNCaP cells (180). Of major importance in our
previous data is that the overall antiproliferative effects of ASO-miR-221 were
significant in pancreatic tumor cells (Figures 3.3). However, contrary to our expectations,
there was no cell cycle change after transfection of anti-miR-221 in HS766T cells even
though transfection of ASO-miR-221 reduced the amount of miR-221 (Figure 3.3D) and
the level of p27(kip1) protein was increased after 48 h of treatment (Figure 4.3B). This
result may be due to the possibility that apoptosis induced by ASO-miR-221 was cell
cycle independent and apoptotic cells mainly emerged from the G1 phase and therefore
resulted in no net changes in the cell cycle pattern. Also, bioinformatic analyses suggest
a great number of predicted targets of miRNA-221. If inhibition of miR-221 by antisense
may increase some proteins impairing p27(kip1) nuclear import in pancreatic cancer cells
in our experimental conditions, cell cycle arrest effects could be overcome.
ASO-miR-212 significantly blocked G2/M phase with abrogation of G1 phase
and ASO-miR-301 induced apoptosis without arresting the cell cycle (Figure 4.1 and 4.2).
These observations made us further investigate and elucidate the miRNA target genes
related with cell cycle and apoptosis mechanisms in pancreatic tumor cells. In this study,
we identified FOXO3a as a target gene of miR-212.
89
Forkhead box O transcription factors are the mammalian orthologs of C.elegans

transcription factor DAF-16 (193, 194) and FOXO3a (FKHRL1) is one of the FOXO
family members (195). FOXO3a is involved in apoptosis, cell cycle regulation, oxidative
stress and longevity (189, 196, 197). It is reported that FOXO3a functioned as a tumor
suppressive gene causing cell death in several cancers (198-200). For example, FOXO3a
inhibits estrogen-dependent breast cancer cell growth and tumorigenesis (198) and the
transcription factor RUNX3, which mediates apoptosis and cell growth inhibition in
gastric epithelial cells, physically interacts with the FOXO3a and induces apoptosis,
activating Bim (199). Also, Chen et al. (200) demonstrated that the accumulation of
FOXO3a increased radiosensitivity and patients survival was enhanced. FOXO3a also
regulates cell cycle. It arrests the G1/S phase during the cell cycle either in a p27(kip1)dependent (201) or p27(kip1)-independent (202, 203) manner. In addition, FOXO3a
induces G2/M phase delay or arrest through the GADD45a (growth arrest and DNA
damage inducible alpha protein) or cyclin G2 (189, 204). FOXO3a directly binds and
activates the promoter of GADD45a (189, 204).
Tran et al. also used GADD45a-
deficient fibroblasts demonstrating that G2/M arrest by FOXO3a at least partly requires
GADD45a (204). In contrast, one paper showed that GADD45a is overexpressed in
pancreatic adenocarcinoma and it enhances cell proliferation in pancreatic cancer (205).
Yamasawa et al. reported that GADD45a is expressed in 54.2% of resectable invasive
ductal carcinomas (IDCs) of the pancreas and 13.6% of IDC patients showed a point
mutation in the GADD45a gene (206). These two results are contradictory with other
reports and our results.
However, they did not explain the further mechanism of
90
overexpressed GADD45a and the expression of GADD45a in those two cases might be
affected by other upstream signaling or other miRNAs.
To date, no one has identified a target gene of miR-212. Here, we demonstrated,
for the first time, that ASO-miR-212 induced apoptosis and caused G2/M arrest during
the cell cycle (Figure 4.1 and 4.2D), regulating the FOXO3a expression at the RNA and
protein level (Figure 4.4 and 4.5). We are currently investigating the downstream gene
that might be affected by the FOXO3a to better explain the mechanism of apoptosis and
cell cycle. We expect that GADD45a or cyclin G2 are possible downstream genes to
explain G2/M arrest and Bim is a candidate for apoptotsis. Moreover, the G2/M arrest
was more significant in Panc-1 cells compared to HS766T after ASO-miR-212 treatment
(unpublished data). Therefore, the correlation of GADD45a level and the effect of G2/M
arrest between Panc-1 and HS766T will be studied and these results may explain why
miR-212 causes a cell cycle arrest and apoptosis in pancreatic cancer.
Supported by an NIH grant CA107435 to T.D.S. and a grand from the National
Pancreas Foundation.
We thank Christine Esau at ISIS Pharmaceuticals for her
assistance with the Antisense oligonucleotides and also thank Jong-Kook Park with
experiments assistance.
91
Figure 4.1. Flow cytometric analysis of the effect of antisense oligonucleotide (ASO)
on cell cycle of HS766T cells.
Histograms shown here represent 10,000 events and the percentage of each cycle is
calculated. (A) cell cycle of control oligo, 20 nM for 48 h. (B) cell cycle of ASO-miR-21,
IC50 for 48 h. (C) cell cycle of ASO-miR-301, IC50 for 48 h. (D) cell cycle of ASO-miR212, IC70 for 72 h.
92
**
60
**
50
40
**
30
**
*
Annexin V positive cells (%)
70
20
10
0
24 hr
48 hr
ASO301 (40 nM)
ASO221 (20 nM)
ASO212 (20 nM)
ASO21 (45 nM)
Control oligo (50 nM)
ASO301 (40 nM)
ASO221 (20 nM)
ASO212 (20 nM)
ASO21 (45nM)
Control oligo (50nM)
Figure 4.2. Flow cytometric analysis of apoptosis in HS766T cells.

Apoptosis induction in HS766T cells transfected with control oligo and antisense to miR21, -212, -221, or -301. Cells were treated with an IC70 concentration of each ASO.
Cells were harvested after 24 and 48 h of incubation and subsequently assayed for
apoptosis induction by flow cytometry using Annexin V-FITC staining.
* P < 0.05; ** P < 0.01 (Students t-test).
93
Figure 4.3. Western blot analysis of PTEN, RECK and p27(kip1) in HS766T cells
treated with antisense to miR-21 or miR-221.
Increased protein expression of the predicted targets to (A) miR-21(PTEN), miR-21
(RECK) and (B) miR-221(p27(kip1)) are shown. Numerical values represent the relative
levels of each protein to control protein (actin) as determined by densitometry.
94
(A)
(B)
7e-7
Relative FOXO3a mRNA level
6e-7
5e-7
4e-7
3e-7
2e-7
1e-7
ASO212_48h
ASO212_24h
control oligo_48h
control oligo_24h
lipo control_48h
lipo control_24h
untreatment_24h
Figure 4.4. Alignment of the FOXO3a binding site with miR-212 and mRNA
expression of FOXO3a after ASO treatment.
(A) Seed region complementarity between miR-212 and FOXO3a 3UTR and
conservation of FOXO3a binding site across species. (B) The relative gene expression of
FOXO3a was measured after transfection. 20 nM of control oligo and ASO-miR-212
were used.
95
96
Figure 4.5. Target validations by luciferase assay and FOXO3a western blotting.
(A) Renilla luciferase activity was normalized by firefly luciferase and cells were treated for 24 h. FOXO3a protein levels were
evaluated after ASO-miR-212 transfection at various time points (B) and transfecting pre-miR-negative (N) and pre-miR-212 (P) at 30
nM (C) and 60 nM (D). Numerical values represent the relative levels of each protein to control protein (actin) as determined by
densitometry.
CHAPTER 5
SYSTEMATIC EVALUATION OF microRNA PROCESSING PATTERNS

IN TISSUES, CELL LINES AND TUMORS
5.1 INTRODUCTION
The genes encoding microRNAs (miRNAs) are located within introns, intergenic
regions, and exons (15). The primary RNA transcript containing the miRNA primary
precursor (pri-miRNA) is processed in the nucleus by Drosha/DGCR8 to produce the
precursor miRNA (pre-miRNA) (19). pre-miRNA is ~70 nt in length and contains the
characteristic hairpin structure.
The pre-miRNA is transported to the cytoplasm by
Exportin 5 (207), where it is subsequently processed by Dicer to the ~21 nt mature

miRNA.
Very little is known regarding the regulation of miRNA processing. Conceivably,
regulation of miRNA biogenesis may occur at the level of Drosha processing, nuclear
transport, or Dicer processing. Several studies have shown that miRNAs are present at
the level of precursor but are not processed to the mature miRNA. Michael et al. (76)
used Northern blots to show that the precursors to miR-143 and miR-145 are expressed in
colorectal tissues and tumors; however, the mature miRNA was detectable only in the
97
normal colorectal tissue. Pre-miR-138-2 was shown by Northern blotting and in situ
hybridization to be expressed in HeLa cells, but the mature miR-138 was undetectable
(43). By using published mRNA data to approximate the miRNA precursor expression
encoded within introns, Thomson et al. (208) showed widespread down-regulation of
miRNAs in cancer.
To our knowledge, no one has addressed the issue of relative
differences in precursor and mature miRNA expression by large-scale profiling. This

may be due to the fact that the majority of miRNA studies have focused on quantifying
the active mature miRNA and not the miRNA precursors.
In 2004, the Schmittgen laboratory developed the first real-time RT PCR method
to quantify miRNA (59). This method quantified the miRNA precursors with the goal of
predicting the expression of mature miRNA. By designing primers to the stem of the
miRNA hairpin, the primary precursor and precursor miRNA were simultaneously
quantified (59). This technology has been applied to profile the expression of over 200
miRNA precursors in a number of human cancer cell lines (59, 61, 209) and tissues from
patients with pancreatic cancer (64). In 2005, a TaqMan-based RT-PCR assay was
designed to quantify the mature miRNA (60). PCR and Northern blotting demonstrated
that the expression of mature and precursor miRNAs generally correlate (59, 61, 64, 110,
209). However, precursors to miR-1, miR-122a, and miR-133 showed considerable
expression by PCR, yet mature miRNA was undetectable by Northern blotting (61). This
prompted us to ask the following question: How widespread are alterations in miRNA
biogenesis in different tissues, tumors, or cancer cells lines? To address this question, we
profiled the expression of over 200 precursor and mature miRNAs using sensitive
realtime PCR assays. Our results demonstrate that a wide discrepancy exists between the
98
levels of precursor and mature miRNA, suggesting that unknown mechanisms that
control processing play a critical role in regulating the expression of active, mature
miRNA.
5.2.1 Cells, tissues and RNA

Human cancer cell lines were obtained from ATCC or other investigators.
Human total RNA from 20 tissues was from Ambion. CD19+ human B cells were
purchased from AllCells. RNA from pancreas and liver tumor and adjacent benign
pancreas were obtained from our prior studies (64, 210). RNA was isolated from all
samples using Trizol per the manufacturers instructions. RNA purchased from Ambion
was isolated using ToTALLY RNA (Ambion). Both Trizol and ToTALLY RNA are
similar to the widely used guanidine thiocyanate, acid phenol:chloroform procedure of
Chomczynski and Sacchi (211) and will contain mature miRNA. RNA integrity was
evaluated by analyzing 50100 ng of total RNA using the Agilent 2100 Bioanalyzer.
5.2.2 Real-time RT PCR, miRNA precursors, and mature miRNA

Expression data on 205 miRNA precursors in human cancer cell lines, pancreas,
and liver tissues were as previously reported (61, 64, 210). The expression of 201
miRNA precursors in the 21 normal human tissues was determined using real-time RT
PCR as previously described (59, 61). Mature miRNA expression in the normal tissues,
cell lines, pancreas, and liver tissues was determined using a real-time PCR assay to
99
mature miRNA as described (60) with several modifications.
cDNA to the mature
miRNA was synthesized using gene specific looped primers. Primers were grouped into
five portions of 48-plex each. Mature miRNAs were selected only to match the miRNAs
on which we had precursor data. Five different cDNAs were synthesized for each sample
using one of the five RT 48 plex primer mix as described (60).
5.2.3 Real-time RT PCR, absolute copy number for precursor and mature miRNA
Absolute copy number of miR-155 precursor was determined by constructing a
standard curve of pre-miR-155.
Pre-miRNA RNA was synthesized using in vitro
transcription using primers that annealed to ~50 base pairs (bp) upstream of and downstream from the miR-155 hairpin. The T7 promoter sequence was added to 59 end of the
forward primer. Amplification of the pri-miRNA gene was performed by amplifying
genomic DNA (Roche) using standard procedures.
The PCR product served as a
template for in vitro transcription. The transcription product was gel purified, and the
concentration was measured using a Nanodrop spectrophotometer.
The reverse
transcription reaction was performed as described above, and standard curves of the log
of pre-miRNA-155 copy number versus the CT were made. We also generated a standard
curve for mature miR-155 as described (60). A minimum of 10 copies/cell was used as
the selection criteria for cutoff. The raw data set for the precursor and mature miRNA
expression levels is presented in the Appendix Table C.1-C.4.
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5.2.4 Northern blotting

Northern blotting was performed as previously described (2).
LNA probes
specific to the mature miRNA were purchased from Exiqon.
5.2.5 In situ detection of miRNA

RT in situ PCR to detect the miRNA precursors was used as previously described
(64). The identical primers used to detect the miRNA precursors by real-time PCR were
used for the in situ RT-PCR. The only exception was miR-31; solution PCR used miR31 primary precursor primers, while the in situ PCR used precursor miR-31 primers. The
chromogen is nitroblue tetrazolium and bromochloroindolyl phosphate with nuclear fast
red as the counterstain. Therefore positive signal will appear blue, and a negative signal
will appear pink. Mature miRNA was detected by in situ hybridization using LNA
probes specific to the mature miRNA (Exiqon).
5.2.6 Data analysis

Pearson product moment correlation coefficients were determined for each pair of
precursor and mature miRNA using Microsoft Excel. The significance (probability) of
the correlation coefficients for each pair was determined from the t-statistic.
The
probability of the t-statistic indicates whether the observed correlation coefficient

occurred by chance if the true correlation is zero. Correlation coefficient was considered
to be significantly different from zero at the 0.05 level (P 0.05). P values for all of the
correlations are located in the Appendix Table C.5 and C.6.
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5.3 RESULTS
The expression of 201 mature and precursor miRNAs was profiled in a panel of
22 human tissues, 37 human cancer cell lines, and pancreas and liver tumors/tissues using
real-time PCR assays specific to each class of miRNA (Appendix Table C.1-C.4).
Expression of the active, mature miRNA was either broadly expressed in each of the 22
tissues or was expressed among one, two, or three different tissues. Our mature miRNA
data in tissues closely resembled that of Liang et al. (212), who used the identical
TaqMan assay to profile miRNA in 40 normal human tissues. miRNA expression in the
tissues is designated as ubiquitous, tissue specific, or tissue enriched (Table 5.1).
Ubiquitous expression is designated as those mature miRNAs that are broadly expressed
in all or most tissues. Tissue-specific expression was defined as those miRNAs whose
expression is 20-fold or higher in the specific tissues compared with the mean of the
other tissues. For many specific miRNAs, the expression in the nonspecific tissues was
undetectable, so it was not possible to calculate a foldchange.
The tissue-enriched
designation was reserved for mature miRNAs whose expression was less than 20-fold in
the enriched tissues compared with the mean of the other tissues. Tissues expressing the
most specific miRNAs include brain, heart, skeletal muscle, and pancreas (Table 5.1).
Several miRNAs have the ability to process different mature miRNAs from either
end of the same precursor. The mature miRNA is referred to as 5 or 3 (e.g., miR-1425p and miR-142-3p) or by the star designation (e.g., miR-9 and mir-9) (12). TaqMan
assays were available to quantify 10 of these miRNAs, thus enabling us to study their
processing patterns in a variety of cell lines and tissues. Interestingly, the expression of
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both the 5 and 3 mature miRNA was detectable in most of the cell lines and tissues with
no apparent preference to process one or the other miRNA (Table 5.2).
We also
compared the relative expression levels of both 5 and 3 miRNA. For some miRNAs,
the 5 was expressed at somewhat higher amounts, and for other miRNAs, expression of
the 3 was more predominate (Table 5.2). In some situations, only one of the two mature
miRNAs was detectable, for example, miR-302a and miR-302c but not miR-302a or
miR-302c (Table 5.2); however, this was rare. Asymmetric RISC assembly accounts
for one strand of the miRNA to accumulate (i.e., guide strand) and the other strand (i.e.,
passenger strand) to be degraded (31). Asymmetric RISC assembly likely accounts for
one of the two mature miRNAs to accumulate at slightly higher levels. However our data
demonstrate that both the 5 and 3 miRNAs accumulate in tissues at significant levels,
adding to the complexity of the activity of miRNAs.
The correlation between the expression levels of the precursor and mature
miRNAs was determined from the Pearson coefficient. The Pearson coefficient varied
widely among different cells/tissues and miRNA. For many miRNAs, the Pearsons
correlation was > 0.6 (P < 0.05), demonstrating good correlation between the expression
of precursor and mature miRNA (Appendix Table C.5 and C.6). For other comparisons,
the Pearson value was near zero or negative, demonstrating that the expression of the
miRNA precursor did not correlate to the mature miRNA. miR-100 is an example of a
miRNA with good correlation between precursor and mature miRNA levels in the cell
lines, tissues, and tumors (Figure 5.1 AC). One hundred twenty-five miRNAs displayed
good correlation (Pearsons 0.3, P < 0.05) between the expression of the precursor and
mature miRNA (Appendix Table C.5 and C.6).
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Other situations displayed good correlation between the expression of precursor

and mature miRNA in specific tissues, while the expression of both precursor and mature
miRNA in the other tissues was very low or undetectable. These tend to be the tissue
specific miRNAs listed in Table 5.1 and were more prevalent among the tissue panel than
in the cell lines. Examples include miR-137 and miR-9 in brain and miR-1-1 in skeletal
muscle and heart (Figure 5.1 DF).
Still other situations exist where high amounts of precursor were present in many
samples, yet mature miRNA was undetectable or had very low expression. The precursor
to the brain-specific miR-128a was expressed predominately in brain tissue, yet it was
expressed at ~100 copies per cell in many tissues (Figure 5.2A). Expression of the
mature miRNA was detectable only in brain and skeletal muscle (Figure 5.2B). miR128a precursor was present in all of the cancer cell lines at levels of several hundred
copies per cell, yet mature miR-128a was not expressed in any of these cell lines (Figure
5.3B). miR-105 had detectable precursor expression, at several hundred copies per cell,
in HS766T, Panc-1, MiaPaca-2, HCT-116, HeLa, and HL-60 cells, but these cells had
undetectable mature miRNA (Figure 5.3A).
The precursor PCR assay simultaneously measures the pri- and pre-miRNA (59).
We asked the following question: Is the mature miRNA absent in some of these cell lines
because the pri-miRNA is not processed by Drosha to the pre-miRNA? To answer this
question, we used Northern blotting to detect the pre-miRNA. Our experiments required
the enhanced sensitivity of LNA probes to visualize many of the miRNA precursors. The
precursor to miR-128a was present in four cancer cell lines and mouse brain; however,
the mature miR-128a was present only in mouse brain (Figure 5.3D). Precursor to miR104
105 was clearly visible by Northern blotting in 6 cancer cell lines, but mature miR-105
was not (Figure 5.3C).
miR-31 was of particular interest because it displayed the greatest amount of
differential processing of any of the miRNAs studied. This differential processing was
validated by both real-time PCR and Northern blotting (Figure 5.4). Mature miR-31 was
detectable by real-time PCR and was clearly visible by Northern blotting in the cancer
cell lines HS766T and PL45 (pancreas) and HT-29 (colorectal) but not in the
hematological cell lines (K562 or HL-60) (Figure 5.4C,D).
Mature miR-31 was
detectable at low to high levels in A549, HOP62, H719, MDA231, HeLa, PC3, and
HCT8 cells but was undetectable in MCF-7, LNCaP, and Daudi cells (Figure 5.4).
Interestingly the miR-31 precursor was detectable by Northern blotting in each of these
cell lines (Figure 5.4C,D). Our data clearly demonstrate situations where the miRNA
primary precursors are transcribed and processed to the precursor miRNA yet are not
processed to mature miRNA.
Precursor miRNA is generated by Drosha in the nucleus and then transported to
the cytoplasm by Exportin 5. Since Dicer processing occurs in the cytoplasm, we asked
the following question: Are certain miRNA precursors not processed to the mature
miRNA because they remain in the nucleus rather than being transported to the
cytoplasm? To answer this question, we applied in situ RT-PCR and in situ hybridization
to detect the precursor and mature miRNAs, respectively.
For miRNAs that are
processed to mature (e.g., miR-31 in HS766T cells), the precursor staining was apparent
in both the nucleus and cytoplasm, while the mature miRNA was present only in the
cytoplasm (Figure 5.4E,F). For miRNAs that are not processed to the mature form, the
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precursor staining was primarily nuclear and/or nucleolar (Figure 5.3E,F and Figure
5.4G). This phenomena is particularly striking for miR-31 precursor expression in MCF7 cells; extensive positive staining for pre-mir-31 was apparent in the nucleolus only
(Figure 5.4G). MCF-7 cells are negative for mature mir-31 (Figure 5.4H). For miR-31,
miR-105, and miR-128a in these cell lines, processing to the mature form does not
proceed because the miRNA precursor is retained in the nucleus. The in situ PCR signal
is for the precursor miRNA (as well as the primary precursor) because the precursor
miRNAs were detected by Northern blotting (Figure 5.3 and 5.4).
The Pearson correlation was compared among the cell lines, tissues, and tumors
by plotting the frequency versus the Pearson coefficient (Figure 5.5). The cell lines
produced a unimodal distribution centering near zero (Figure 5.5A). The histogram for
the 22 normal tissues produced a bimodal distribution with one mode centered near zero
and the other greater than zero (Figure 5.5B).
The pancreas and liver tissues also
produced conflicting histograms, with the liver specimens displaying a unimodal

distribution centering at zero while the pancreas tissues were bimodal (Figure 5.5C,D). A
high positive Pearson correlation demonstrates that the expression of precursor and
mature miRNAs are directly proportional and suggests efficient processing of precursor
to mature miRNA. A low Pearson coefficient suggests no relationship between the two,
one explanation being that precursors are not being processed to mature miRNA. A
negative Pearson suggests an inverse relationship between mature and precursor miRNA.
It should be emphasized that of over 800 comparisons, only 27 negative Pearson
coefficients were significant (P < 0.05). Our data suggest that processing of many
precursor miRNAs is somehow deficient or defective in cancer cell lines and liver tissues.
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However in 22 normal tissues and pancreas tissues, there is a population of miRNAs that
are processed efficiently. This trend parallels the miRNA expression pattern in tumors.
In pancreas cancer, there is a predominate increase in the expression of the miRNA (64),
while in liver cancer, the opposite is true (81, 210). This suggests that in pancreatic
cancer, the increased expression of miRNA is regulated at the level of transcription with
no apparent alterations in processing, but in liver cancer, the reduced expression of
miRNA in the tumors results from a reduction in miRNA processing.
It was recently demonstrated that mutations (153) or SNPs (213) within the
miRNA precursors can cause interference with miRNA processing. While we believed
this to be an unlikely explanation to describe the lack of miRNA processing, since the
phenomena was so widespread (Figure 5.3 and 5.4), we sequenced +100 bp on either side
of the miR-31 hairpin in HeLa and MCF-7 cell lines to look for evidence of mutations or
SNPs. The sequences were unchanged compared with wild type, demonstrating that
reduced levels of mature Mir-31 in these cells was not due to reduced processing caused
by a mutation or SNP.
5.4 DISCUSSION
The Pearsons correlation data along with the relative expression levels of
precursor and mature miRNA demonstrated that biogenesis of miRNAs fit into different
categories. In one group of miRNAs, the precursor and mature miRNAs are expressed in
most tissues and cell lines, and the precursor expression correlates with the mature
miRNA expression. These miRNAs typically had a Pearson correlation coefficient > 0.3
107
and P < 0.05. Examples of these miRNAs include many of the ubiquitously expressed
miRNAs such as miR-100, miR-155, and miR-125a. Expression of these miRNAs would
presumably be regulated by transcription alone with no regulation of processing events
(i.e., all miRNA expressed as primary transcripts are processed to mature miRNA).
Another group of miRNAs, such as many of the tissuespecific miRNAs, have a
high amount of expression of both the precursor and mature in specific tissues only. So
expression of these miRNAs is regulated by transcription in specific tissues with no
apparent regulation of miRNA biogenesis. Examples of these miRNAs include miR-9,
miR-1, and miR-216.
Still another group of miRNAs expressed the precursor at
significant levels in all or most of the cells or tissues; however, the mature miRNA is not
present. Examples include miR-105, miR-128a, and miR-31 in cancer cell lines (Figure
5.3 and 5.4) and represent miRNAs that are expressed at the primary transcript level yet
for some unknown reason are unable to be processed to the mature miRNA.
Our data clearly show that a large number of miRNAs are transcribed as
precursors but are not processed to mature miRNA.
Conceivably any of the steps
involved in miRNA biogenesis (nuclear transport or processing by Drosha or Dicer)

could be involved to reduce the mature miRNA levels. Reduced levels of Dicer were
shown in lung tumors (214), and many of the cancer cell lines studied here had reduced
mature miRNA. It is unlikely that a whole-scale reduction in Drosha or Dicer processing
accounts for the reduced levels of mature miRNA in cells that express the miRNA
precursors since other miRNAs are efficiently processed to mature in these same cell
lines or tissues. One explanation to account for reduced nuclear transport or processing
by Drosha or Dicer in some miRNAs but not others is that other specific factors bind to
108
the miRNA precursors and either promote or interfere with processing or transport. In
fact, this inhibitory model was proposed by Obernosterer et al. (43) to account for a lack
of mature miR-138 in HeLa cells. It was recently shown that hnRNP A1 is required for
Drosha processing of miR-18 from the primary precursor (40).
Conceivably, other
hnRNPs may be involved in regulating steps of miRNA biogenesis. While we did not
use Northern blotting and in situ hybridization to study all of the miRNAs with reduced
processing, the ones that were studied in several cancer cell lines showed that the miRNA
was processed to the precursor but was not transported from the nucleus to the cytoplasm.
This suggests an intriguing, unknown mechanism to guide the subcellular distribution of
miRNA precursors and prevent them from being processed to the active mature miRNA.
It is our hope that with the availability of this data set, many future studies will be
initiated to unravel the various mechanisms that control miRNA biogenesis.
5.5 ACKNOWLEDGMENTS
We thank Caifu Chen at Applied Biosystems for his assistance with the TaqMan
microRNA assays and also thank Gerard Nuovo for performing In situ RT PCR. This
work was supported by grant R21 CA114304 to T.D.S.
109
Figure 5.1. microRNAs with good correlation in expression levels of precursor and
mature.
The expression of the precursor and mature miRNAs was determined using real-time
PCR assays specific to each class of RNA. Plots of the correlation between the precursor
and mature miR-100 in a panel of 22 normal tissues (A), 37 cancer cell lines (B), and the
pancreas and liver tumors and normal tissues (C) are shown along with the Pearson
correlation coefficient. Also shown is the correlation in the expression of precursor and
mature miRNAs in the panel of 22 normal tissues for miR-1 (D), miR-137 (E), and miR9 (F).
110
Figure 5.2. Expression of miR-128a in 22 normal tissues and cells.

The expression of miR-128a precursor (A) and mature miR-128a (B) was determined by
quantitative real-time PCR in 22 normal human tissues and cells.
111
Figure 5.3. Lack of microRNA processing in cancer cell lines.

The expression of precursor and mature miR-105 (A) and miR-128a (B) was assayed in
several human cancer cell lines by real-time PCR. mir-128a was also detected in mouse
brain tissue. Detection of the primary precursor (black columns) was apparent in all cell
lines; expression of mature miRNA (red column) was detectable only in the brain tissue.
With the exception of mouse brain tissue, Northern blots to miR-105(C) and miR-128a
(D) reveal the presence of the precursor only. Cell lines are the same as shown for the
quantitative PCR. In situ RT-PCR results for pre-miR-105 in HeLa cells (E, note intense
nuclear localization) and pre-miR-128a in HS766T cells (F).
112
Figure 5.4. miR-31 expression in cancer cell lines.

The expression of precursor and mature miR-31 was assayed in 15 cancer cell lines.
Detection of the primary precursor (black columns) was apparent in all cell lines by realtime RT-PCR (A, B); however, the mature miR-31(red columns) was present at varying
levels or not at all. (C, D) Northern blot confirmation of precursor and mature miR-31 in
cell lines. Note the presence of the ~70-nt precursor in each of the cell lines. Each
column of the Northern blot corresponds to the identical cell lines listed in panels A and
B. In situ RT-PCR to miR-31 precursor in HS766T cells (E) and MCF-7 cells (G). In
situ hybridization using an LNA probe to mature miR-31 in HS766T cells (F) and MCF-7
cells (H). Magnification, X400.
113
Figure 5.5. Pearson correlation among the various samples studied.

The frequency of the Pearsons correlation coefficient describing the relationship
between the precursor and mature miRNA expression is plotted for the cell lines (A), 22
normal tissues (B), normal pancreas and pancreas tumors (C), and normal liver and liver
tumors (D).
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Tissue specific expression.a miR-122a (liver); miR-124a (brain); miR-128a (brain, skeletal muscle); miR-216, miR-217
(pancreas); miR-219 (brain); miR-302a, miR-302c (heart); miR-367 (heart); miR-373 (placenta)
Tissue specific expression.b miR-1 (heart, skeletal muscle); miR-7 (brain, pancreas, thyroid); miR-95 (skeletal muscle);
miR-133a (heart, skeletal muscle); miR-149, miR-153 (brain); miR-203 (esophagus); miR-204
(kidney); miR-206 (skeletal muscle); miR-215 (small intestine); miR-302d (heart); miR-323,
miR-330, miR-346 (brain); miR-371, miR-372 (placenta); miR-375 (pancreas)
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Tissue enriched expression.c let-7i (thyroid); miR-148a (pancreas); miR-150 (spleen, thymus, B cell); miR-181a (thymus);
miR-181b, miR-181c (brain, thymus); miR-190 (kidney); miR-192, miR-194 (colon, smallintestine); miR-196b (cervix); miR-205 (esophagus, prostate, thymus); miR-213 (brain,
thymus); miR-223 (lung, spleen); miR-328, miR-338, miR-340 (brain); miR-376a (placenta)
Not expressed in any of the 22 tissues studied. miR-220, miR-302a, miR-302c, miR-384
Expression of 202 mature miRNAs was profiled in 22 human tissues and cells using real-time PCR as described in Materials
and Methods.
a
Mature miRNA expression is detectable at sufficient levels in the specific tissue but is undetectable in all or most of the
remaining tissues.
b
Mature miRNA expression is >20-fold higher in the specific tissue(s) compared with the mean of the other tissues.
c
Mature miRNA expression is <20-fold higher in the enriched tissue(s) compared with the mean of the other tissues.
Table 5.1. microRNA that display tissue-specific or tissue enriched expression.
miRNA (5 and 3)a
Expression ratio (5 miRNA/3 miRNA)b
miR-9, miR-9
4.40
miR-175p, miR-17-3p
25.7
miR-126, miR-126
-10.8
miR-1425p, miR-142-3p
-13.7
miR-154, miR-154
Not expressed
miR-199a, miR-199a
-162.4
miR-302a, miR-302a
miR-302a only expressed in heart
miR-302c, miR-302c
miR-302c only expressed in heart
miR-3245p, miR-324-3p
-3.0
miR-3805p, miR-380-3p
miR-3803p not expressed
Mature miRNAs are listed in the order as they appear within the miRNA precursor
in the 5 (5p) to 3 (3p) orientation.
b
The mean expression of the mature miRNA and miRNA was determined in a
panel of 22 human tissues. The expression ratio is presented as the mature miRNA
that is located on the 5 end of the miRNA precursor compared to that which is 3
on the end.
Table 5.2. Relative expression levels of mature miRNA and miRNA star forms.
116
CHAPTER 6
SUMMARY AND DISCUSSION
The goal of this dissertation was to identify the most important microRNA
(miRNA) that can be used for diagnosis and treatment of pancreatic cancer.
We
hypothesized that miRNAs are deregulated in pancreatic cancer and the inhibition of
upregulated miRNAs by antisense oligonucleotide would be a prospective therapy in
pancreatic cancer.
The summary of this dissertation is as follows; Expression profiling of a small
number of miRNAs (~ 200 miRNAs) distinguished pancreatic tumors from
normal/benign pancreas. Large numbers of miRNAs were differentially expressed in
pancreatic adenocarcinoma and the majority was increased in pancreatic cancer. In situ
RT PCR demonstrated that overexpressed miRNAs are restricted to pancreas tumors, not
stroma cells (Chapter 2).
Overexpressed miRNAs were inhibited by antisense
oligonucleotides at low nM concentration and normal pancreatic cell lines (HPNE)

showed less sensitivity to antisense oligonucleotides, suggesting selective target therapy
in pancreatic cancer.
The combination of antisense oligonucleotide with a
chemotherapeutic agent, gemcitabine showed sensitization effect via schedule-dependent

manner and the combination with two antisense oliogonucleotides also showed a
117
synergistic effect (Chapter 3).
In addition, miRNA inhibition by antisense
oligonucleotide caused cell cycle arrest and increased apoptotic cells.
For further
mechanism study, the target molecules of miRNAs that are deregulated in pancreatic
cancer were validated. PTEN and RECK were confirmed as a target of miR-21 and
p27(kip1) was validated as a target of miR-221. FOXO3a was identified as a new target
gene of miR-212 using reporter assay and western blotting (Chapter 4). Lastly, the
processing of miRNA was studied in various normal tissues, patients tumor tissues and
cancer cell lines. A number of miRNAs showed tissue-specific expression and the
miRNA processing pattern was different in various samples (Chapter 5).
There are a number of evidences that miRNAs are differentially expressed in
many human cancers (reviewed in Chapter 1). Our study suggested that overexpressed
miRNAs in pancreatic cancer could be used as a therapeutic agent and diagnostic in
pancreatic cancer.
Although many miRNAs that were differentially expressed in
pancreatic cancer were also implicated in other type of cancers (i.e. miR-15, 16, 21, 155
and 221), several other miRNAs such as miR-212 and 301 appear to be deregulated
specifically in pancreatic cancer.
Also, the cell proliferation assay using antisense
oligonucleotide indicated that low nM range of IC50 is effective to show cell cytotoxicity,
specifically in pancreatic cancer cells, not normal pancreas cells. These data indicate that
selective therapy targeting the miRNAs is promising. To better understand the miRNA
function, miRNA targets need to be validated. Here, one target molecule of miR-212
(FOXO3a) was identified possibly affecting cell cycle arrest and apoptosis in cancer cells.
Since one miRNA targets numerous mRNAs, it is a big challenge to identify targets and
to understand the network between miRNA and mRNA. Even, miRNA processing was
118
impaired in certain samples or conditions, implying more complicated function of

miRNAs. This study is the first to show miRNA inhibition by antisense oligonucleotide
in pancreatic cancer and to identify a target gene of miR-212 in pancreatic cancer.
The possibility of miRNAs as a therapeutic agent was some what confirmed in
our studies. The inhibition of miRNAs by ASO showed that reduced cell proliferation,
cell cycle arrest and increased apoptotic cells in vitro.
Even though we havent
confirmed yet whether ASO inhibits those miRNAs efficiently in vivo, Krtzfeldt et al.
demonstrated that cholesterol conjugated antisense inhibited liver specific miR-122 in
mouse for 23 days (127). Currently, modified antisense has been developed for stable
transfection in vivo and if antisense is conjugated with molecules that are present only in
the pancreas it might be a new form of selective targeting to pancreatic tissue.
Even though miRNAs are important targets for therapeutic purposes, miRNAs
can also be used for diagnostic purposes. The survival rate in pancreatic cancer is very
low because most pancreatic cancers are diagnosed at very late, untreatable stage. From
our study, miRNAs that are overexpressed in pancreatic cancer could be a promising
diagnosis marker in pancreatic cancer.
One of the problems of diagnosing in pancreatic cancer is distinguishing chronic
pancreatitis from pancreatic cancer. It is reported that 5% ~ 10% of resected pancreatic
tissues are eventually diagnosed as pancreatitis, not as pancreatic cancer (216).
Pancreatic cancer and chronic pancreatitis show similar symptoms and clinical data (217).
Chronic pancreatitis contains abundant stroma, similar to pancreatic cancer (146), but the
treatment of chronic pancreatitis is very different from pancreatic cancer. In our study,
we profiled ~ 200 precursor miRNAs on 4 chronic pancreatitis samples to see if miRNA
119
expression is different from cancer.
Clustering data (Figure 2.2) showed chronic
pancreatitis is separated from the cancer group and terrain map (Figure 2.3) showed
chronic pancreatitis lie in between the normal pancreas and tumor. This result suggests
that miRNA may be a good marker to distinguish the pancreatic cancer and chronic
pancreatitis.
Mature miRNA profiling data with sufficient samples are needed and
microdissected tumor and stroma samples may provide a better miRNA marker for
chronic pancreatitis and pancreatic cancer.
In addition, pancreatic juice has been used to investigate the genetic markers of
pancreatic cancer (218).
Pancreatic juice can be collected in the duodenum during
routine upper gastrointestinal endoscopy after secretin stimulation (219). No one has
systemically profiled miRNAs in pancreatic juice samples and this method will be an
easier diagnostic for patients compared to tissue biopsy to screen the cancer marker.
The pancreatic tissue and juice samples are directly obtained from pancreas, but
tissue biopsy or GI endoscopy is not a convenient or economic procedure for the patient.
Serum is the ideal clinical specimen because it is easily obtained from patients and once a
diagnostic assay is developed, patients can have more frequent examination for early
diagnosis. However, serum samples are not from direct tissue, so pancreatic tissue or
juice might show more accurate diagnostic marker, which means a more sensitive serum
marker is needed. As mentioned in Chapter 3, serum marker CA19-9 (sialylated Lewis
blood group antigen) has been used, but the usage is limited because it shows a high
false-positive rate and some patients do not express Lewis antigens (154-156). Recently,
serum macrophage inhibitory cytokine 1 (MIC-1) seems to be a better marker of
pancreatic cancer than CA19-9 (157). However, MIC-1 is not better than CA19-9 in
120
discriminating chronic pancreatitis patients from pancreatic cancer patients (157). Our
study showed that miR-216, 217 and 375 were specifically expressed in pancreas
(Chapter 5) and miR-212 and 301 were overexpressed in pancreatic cancer (Chapter 2).
If these miRNAs are differentially expressed in patients serum samples, this might be the
most sensitive and accurate diagnostic marker for pancreatic cancer.
Lastly, pancreatic cancer precursor lesions can be useful for early detection of
cancer. Pancreatic ductal adenocarcinomas arise from noninvasive epithelial precursor
lesions and one of the epithelial precursors in the pancreas is pancreatic intraepithelial
neoplasm (PanINs), which can be used for a pancreatic cancer progression model (215).
This model contains a series of precursor lesions from low-grade PanIN-1A to high-grade
PanIN-3. PanINs are common in invasive ductal adenocarcinoma and many of the
genetic changes in cancer-related genes are shown in these lesions (Figure 6.1). PanIN1A is flat epithelial proliferation that doesnt contain architectural or cytologic atypia and
PanIN-1B is similar with PanIN-1A, but shows intraluminal papillae. The mutations of
K-ras and HER-2/neu genes usually occurr in the early stage of PanINs. The tumorsuppressor gene p16 is inactivated in PanIN-2, which shows moderate nuclear and
architectural atypia. In PanIN-3 stage, p53, DPC4, and BRCA2 genes are decreased and
there are significant architectural and cytological atypia that nuclei are enlarged and
overlapping. In our study, the expression of tumor suppressor gene PTEN, RECK, p27
and FOXO3a were repressed by miR-21, 221 and 212. We havent examined which
stage these miRNAs occur. It is possible that these oncogenic miRNAs might function in
late stage of PanINs because tumor suppressor genes such as p16 and p53 are altered in
these stages (Figure 6.1). In addition, overexpressed miRNAs in our study might be
121
targeting other tumor suppressor genes such as p53, DPC4, and BRCA2. To answer
these questions, a murine PanIN model, which are much alike in histology with human
PanIN, could be used to identify specific miRNAs in pancreatic cancer progression.
In conclusion, the study of miRNA expression and function in pancreatic cancer
suggests that miRNAs play an important role in tumor biogenesis and have a potential for
therapeutic and diagnostic agents.
122
Figure 6.1. Pancreatic cancer progression model and genetic alteration.

Oncogenic miRNA-21, 221, and 212 repress tumor suppressor genes that cause cell cycle
arrest and apoptosis and they might be specific miRs in cancer progression and could be
better marker. ? : not confirmed yet.
123
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146
APPENDIX A
DATA RELEVANT TO CHAPTER 2
147
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
Chronic pancreatitis
CP1
0.012767564
0.06314825
0.022277452
0.020109563
0.000543603
0.067560629
CP4
0.004554593
0.024298885
0.007258358
0.008586259
0.000190536
0.016552798
0.090772624
0.001815394
0.02490334
0.016838315
0.006010193
0.041960177
0.001198204
0.00022334
0.066299558
0.042820043
0.030410789
0.016667845
CP6
0.010716357
0.034370636
0.018295382
0.020264444
0.000434601
0.03898443
0.001953556
0.079698693
Normal Pancreas
CP7
0.005650276
0.016007037
0.008624916
0.009398919
0.000198266
0.023946308
0.036982564
0.001042869
0.015056488
0.004408677
9.81398E-05
0.007905583
0.002435421
0.001181303
0.071358573
0.014831626
0.020724227
0.003639678
0.006728574
0.004590641
0.007066112
0.001263922
0.000236127
0.022849619
0.03842686
0.016794899
0.01497907
0.013672666
0.002594387
0.036118865
0.002724717
0.000747346
0.083151717
0.041434789
0.016108008
0.004868059
0.000255121
0.006271364
0.007608744
0.014258866
0.097736996
0.067500597
0.710241994
0.156661987
0.005065515
0.002268153
0.00967714
0.014620986
0.103802955
0.008675703
0.046432247
0.013489642
0.004846943
0.010861246
0.001799911
0.030510124
0.089869873
0.041673089
0.545149137
0.106883375
0.00568522
0.000420431
0.000589615
0.017130381
0.041846625
0.004841798
0.168437418
0.018285629
0.000321186
0.000315185
2.341142206
0.036732593
0.053611733
0.152687733
4.022794578
0.000163262
0.484202999
0.019780181
0.01947956
0.083139473
1.797990526
1.813199124
0.013561291
0.049424267
0.063950297
2.617194909
0.000147853
0.467826363
0.009714151
0.02630918
0.035967509
2.221679286
Ambion1 (N)
Ambion2 (N)
0.03634888
0.005953737
0.002387898
0.034601248
0.00733402
0.004160942
0.002639577
0.013907924
0.001065562
0.006559961
0.001410304
0.009764104
0.011195291
0.000930663
0.017999122
0.002328745
0.001740222
0.236435712
0.164846752
0.056627214
0.000395768
0.009458314
0.006369932
0.004876282
0.00099491
0.020764554
0.007157494
0.008283994
0.338322544
0.257808586
0.072412457
0.001665171
0.009949689
0.009179118
0.006865729
0.019011395
0.101426442
0.055632402
0.253759848
0.008367024
0.02919225
0.000382308
0.000369211
2.001817175
0.021133181
0.013086115
0.037915255
1.262563736
0.007294283
0.015960159
0.010796828
0.069059053
0.054880421
0.241946399
0.099447014
0.023905516
0.000369341
2.956513509
0.019293649
0.009449887
0.14623336
1.213713886
Continued
Table A. microRNA precursor expression in pancreatic tissues and cell lines.

The expression of each miRNA precursor relative to 18S rRNA was determined using
real-time PCR as described in Materials and Methods. The data for each miRNA
precursor was determined using SYBR green detection on all miRNAs except those
with an asterik (*) which were determined using TaqMan probes. The expression of
each mature miRNA relative to 18S rRNA was determined using real-time PCR as
described in Materials and Methods. Data are presented as 2^-CT (CT miRNA - CT
18S rRNA) X 1,000,000. The data on 18S rRNA is the raw CT value.
148
Table A. continued
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
0.014534236
0.010159932
0.061031192
0.043054581
0.002108041
0.036616687
0.002017191
0.004278047
0.066914743
0.00702677
0.0002481
0.024450163
0.021287722
0.003379688
0.060933139
0.024868156
0.002824025
0.024079101
0.009170814
0.002254476
0.030577008
0.016746479
0.000113562
0.009098381
0.057518827
0.004284252
0.043036247
0.038013945
0.01136044
0.000928967
0.001606097
0.00056078
0.028338288
0.036746545
0.009825101
0.005221449
0.043783555
0.013064081
0.000349703
0.001025468
0.000640861
0.006146813
0.069638762
0.021738307
0.011618504
0.044324666
0.015281528
0.004020804
0.011792264
0.001263644
0.00028443
0.004549785
0.008550817
0.135395629
0.395513288
0.00068848
0.052199058
0.011726527
0.001256223
0.067390058
0.113320955
0.008403084
0.000986795
0.002066059
0.000237352
0.166538705
0.200547922
0.000449164
0.020044676
0.002945217
0.000639224
0.025316932
0.040466038
0.013297846
0.029821499
0.161549262
0.005332403
0.000271888
0.013409037
0.074540738
0.008613235
0.037638686
0.028684567
0.000318138
0.031139435
1.443391405
0.01308109
0.00389817
0.04255903
0.05095675
0.000174327
0.007781262
0.00033646
0.018682553
1.322518514
0.01497742
0.003606393
0.053792447
0.073840054
0.040284213
0.004168735
0.000497972
0.007751783
0.000383238
0.00467656
0.000732006
0.005246143
0.122449295
0.282877605
0.000449417
0.050994691
0.012368136
0.001044442
0.057451341
0.059308713
0.004531591
0.000346189
0.028810417
0.169600799
0.01351545
0.000168237
0.000271765
0.028077492
0.020528989
0.002826068
0.010326938
1.338535006
0.003764339
0.002140326
0.039322391
0.056078589
0.000389355
0.000470035
0.000305746
0.000678746
0.058341046
0.150995878
0.000601951
0.031251431
0.010485966
0.000164776
0.015532023
0.068214562
0.002389855
0.000268811
0.009571024
0.08313928
0.002144737
0.000202524
0.001526758
0.004835816
0.000474395
0.012578255
0.652239432
0.012932415
0.001638763
0.012627467
0.012432141
0.001809944
0.010690275
0.001348446
0.091468241
0.020994121
0.001476794
0.025740809
0.000447457
0.005538956
0.042986329
0.011527214
0.189825896
0.006806407
0.00042864
0.034041259
0.004631787
0.026596429
0.003898703
0.087280028
0.028610011
0.000725965
0.006679354
0.000350692
0.006441949
0.06069794
0.092756543
0.006113272
0.19217051
0.001915632
0.000153537
0.007937255
0.002595809
0.000575484
0.010868467
0.026074677
0.000619099
0.691508178
0.348510546
0.004451034
0.051381301
0.003268844
0.000235339
0.151054254
0.203195269
0.013743099
0.001528436
0.035342018
0.231565418
0.008016303
0.001497577
0.005854591
0.068244151
0.008964481
0.001900974
0.107276146
1.228175066
0.011828256
0.008546143
0.285810578
0.252355112
0.001542816
0.005224251
0.018327507
0.003892058
0.548908989
0.420654846
0.002625458
0.029222843
0.021370743
0.00048706
1.752910539
0.140712156
0.005700536
0.000535674
0.080899347
0.15655515
0.012246038
0.000744918
0.00560694
0.055909214
0.012792711
0.003763557
0.024853897
1.193624348
0.005029126
0.008074703
0.214750124
0.177391264
0.001823327
Continued
149
Table A. continued
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
0.025152857
0.02396675
0.018352025
0.000934791
0.00118657
0.006804062
0.000447471
0.001714094
0.025153005
0.006156363
0.021021551
0.019122731
0.000803997
0.002349502
0.002207299
0.00103947
0.000262827
0.016604429
0.010857505
0.00931909
0.018976627
0.020177299
0.001346749
0.020303354
0.000883174
0.001397973
0.020384394
0.004650737
0.003391111
0.01182464
0.005874401
0.00614135
0.007678385
0.001245746
0.00044973
0.006667056
0.032489475
0.048682674
0.136395478
0.001761802
0.017659866
0.003676769
0.000575506
0.00093339
0.106312064
0.011994326
0.023065036
0.255991421
0.007305083
0.005187981
0.082237423
0.001122707
0.004501049
0.00339802
0.00369372
0.000273698
0.031911651
0.0086425
0.008042978
0.001751684
0.000420075
0.022850047
0.003534605
0.010785362
0.004817672
0.000233496
0.024736512
0.005206018
0.000911796
0.00097074
0.013817426
0.115605763
0.016971657
0.013065508
0.001451618
0.002270519
0.002155628
0.037287376
0.015495854
0.003753128
0.012107099
0.008298464
0.00346641
0.009455281
0.006474987
0.016747454
0.001071404
0.114077333
0.000176519
0.013735575
0.265489106
0.249354314
0.001758809
0.002563387
0.017141687
0.008078378
0.006012553
0.009395958
0.001777718
0.019314136
0.002693799
0.000515769
0.003181465
0.045625949
0.015534955
0.001885013
0.011398042
0.007610349
0.014266135
0.006473768
0.011309025
0.014009326
0.000714214
0.063994395
0.002275914
0.004686897
0.205033332
0.343926782
0.003297623
0.000910898
0.00017583
0.020027785
0.000251733
0.00221494
0.008784268
0.004765249
0.113169941
0.028573998
0.000953368
0.011065352
0.007504883
0.013345639
0.014553467
0.014163854
0.00082498
0.044764448
0.000981441
0.047283535
0.28688426
0.131957908
0.003482237
0.001806537
0.019355198
0.166751526
0.012679392
0.00743239
0.013671064
0.00673389
0.006845594
0.007050901
0.047246831
0.069601122
0.026609088
0.00487287
0.005798285
0.000905268
0.140458892
0.002732431
0.006231712
0.002324979
0.049877168
0.000911294
0.057244777
0.264546433
0.192107782
0.001683475
0.002886692
0.000607368
0.004109972
0.005158557
0.002568362
0.323387702
0.004269769
0.002913668
0.056271578
0.120997632
0.00298949
0.000391679
0.022998034
0.011033806
0.00080106
0.366278232
0.009067291
0.003392027
0.022356015
0.198911952
0.001381301
0.000272428
0.000731131
0.000699001
0.002903987
0.000252311
0.033704664
0.000388584
0.003463811
0.002501064
0.039397436
0.002505612
0.015833126
0.000394636
0.039264489
0.000759672
0.015170747
0.25329402
0.238891499
0.000169812
0.000597614
0.005158609
0.000351593
0.064680131
0.000304802
0.001560232
0.013480959
0.00767588
0.001030131
0.001272982
0.006451089
0.000568257
0.055662456
0.000741552
0.001208741
0.001895779
0.046983349
0.004541608
0.001131528
0.033291209
0.00936547
0.001145313
0.003513248
0.013484572
0.000216381
0.011609966
0.000104749
0.002935386
0.000402429
0.095568075
0.004266724
0.003159899
0.142422656
0.310028354
0.000112497
0.000278215
0.005409161
0.000765849
0.021886735
0.000111348
0.001227677
0.001053794
0.012516197
0.000452906
0.061125699
Continued
150
Table A. continued
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
0.298467256
0.027350084
0.094182157
1.040433725
0.005236239
0.001875655
0.008004919
0.004022595
0.002138564
0.000733871
0.09111678
0.073901403
0.170904889
0.002785865
0.067405311
0.005950176
0.290399841
0.000235323
0.008985653
0.000752839
0.000649481
0.009422457
0.010800759
0.000174591
0.025570773
0.003529366
0.015990563
0.010070304
0.01186985
0.009426909
0.000259907
0.002969431
0.007278607
0.503973775
0.042517514
0.00365075
0.00443695
0.002547828
0.204784023
0.001550312
0.018226976
0.25086062
0.000421998
0.067465461
1.097327693
0.00882399
0.002254475
0.000497538
0.012991352
0.003139284
0.000282114
0.007252755
0.044985782
0.006813515
0.009373009
0.003108049
0.06752029
0.005737333
0.066188341
0.000170291
0.008121506
0.000781918
0.000380729
0.001132225
0.000349949
0.000475107
0.014669204
0.012403017
0.017543379
0.007361512
0.006763867
0.009476736
0.000742716
0.000343145
0.001174052
0.572789686
0.014855041
0.008846233
0.004339746
0.000698274
0.133565649
0.002652331
0.022177498
0.003370729
0.005481172
0.308455883
0.004834715
0.013365456
0.19562273
0.166578992
0.016245933
0.068591441
0.927129884
0.005748241
0.002430292
0.092487139
0.001672517
0.02481399
0.357621176
0.005008713
0.001505718
0.012451945
0.000884591
0.004396321
0.000333252
0.000367513
0.043284832
0.050940321
0.115209842
0.00200287
0.067519399
0.012282213
0.135031778
0.000180144
0.01599596
0.006967928
0.000707701
0.012976988
0.005152114
0.001065468
0.012617147
0.026306618
0.028910654
0.00070607
0.033686739
0.004135509
0.053202619
0.020176872
0.002707379
0.015012946
0.000388674
0.000808252
0.006532407
0.00141125
0.001321731
0.001375367
0.726818914
0.00657378
0.01163056
0.002946879
0.002562807
0.124115988
0.003890964
0.013292796
0.000312403
0.002915711
0.004720068
0.207006844
0.001907882
0.000183472
0.00193472
0.001583838
0.003193503
0.011781545
0.000628015
0.002988038
0.021308422
0.005987218
0.004181685
0.000104538
0.00141726
0.70760864
0.020966772
0.002844806
0.002596601
0.000540673
0.060584396
0.004113695
0.00801185
0.000719814
0.000785842
0.104573707
0.433476359
0.006325522
0.156936215
3.561935073
0.008166116
0.007420339
0.000222475
0.065474308
0.000239987
0.002042835
0.00897855
2.13176546
0.277102997
0.019571386
0.013444866
0.107147472
0.000180488
0.023985374
0.011345368
0.001300564
0.003250969
0.001503949
0.0025027
0.002287581
0.024495308
0.004989904
0.039464444
0.002189063
0.002020106
0.014986297
0.004454245
0.005817989
0.019171901
1.844779286
0.071572775
0.057851482
0.018084716
0.003020398
0.519801326
0.003163172
0.084372445
0.001414699
0.030593664
0.000259684
0.124672201
0.360208809
0.015860102
0.150919054
2.349448867
0.013887001
0.006308269
0.000152136
0.04547774
0.000250098
0.006840329
0.002034795
0.754484605
0.124284679
0.024553472
0.001219855
0.121379179
0.005897319
0.796469692
0.00165481
0.00237825
0.000636546
0.002219262
0.003099277
0.0047615
0.000896812
0.015615969
0.007946184
0.041617262
0.000468284
0.001943118
0.010019941
0.001977327
0.006271514
0.006686201
1.4878209
0.124469517
0.0335496
0.008847416
0.006940688
0.137784391
0.013502907
0.119610369
0.034853802
0.00033258
0.05196366
Continued
151
Table A. continued
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
0.049059593
0.006266854
0.038088996
0.04285465
0.00429402
0.0378978
0.00277656
0.060830468
0.015982046
0.011935766
0.05342104
0.005425455
0.019169923
0.018191917
0.002435201
0.056894942
0.010622662
0.270400968
0.092284631
0.0168768
0.003731997
0.031652792
0.000138995
0.009714649
0.023584676
0.000983947
0.005381289
0.001802181
0.002403004
0.00279905
0.064662207
0.010450665
0.017665837
0.015527791
0.067686052
0.003743435
0.025862769
0.001098226
0.002161575
0.003207945
0.003245283
0.000257784
0.073265952
0.06270993
0.001638097
0.003133078
0.09199046
0.144953399
0.028962703
0.000456763
0.048961856
0.011264273
0.000455134
0.000798927
0.001573386
0.020887258
0.055565489
0.002024902
0.001340092
0.036919925
0.007518173
0.001046901
0.076421181
0.053468328
0.001257267
0.00061336
0.049887852
0.009380535
0.000532353
0.000181261
0.000602749
0.036105478
0.080314571
0.001239998
0.000603437
0.040966972
0.00919796
0.000189964
0.000579965
0.004134326
0.001078241
0.001936085
0.004474417
7.1029398
0.001615879
0.001447259
0.002342667
0.003891083
6.620629
0.006772073
0.012702645
0.000832088
BD(N)
ST(N)
OUP2 (B)
OUP16 (B)
0.029860799
0.001374538
0.02020963
0.037667539
0.007318707
0.010710603
0.093053858
0.01802732
0.019361678
0.018450767
0.010403781
0.003561243
0.005969613
0.008935437
7.4282695
0.00062277
0.000189276
0.002401419
0.003617062
0.007596207
0.000207824
7.16372935
Normal Pancreas
Ambion3 (N)
Ambion4 (N)
0.023460053
0.002120054
0.001147644
0.036785208
0.012402666
0.004249536
0.009596833
0.006710613
0.044454914
0.020308574
0.008179125
0.005806966
0.07170906
0.00197598
0.000771986
0.000419186
0.006042031
6.96878565
0.047978933
0.009543253
0.349214863
0.087633717
0.013473494
0.000687387
0.004295533
0.035557566
0.565532315
0.08601612
0.000154308
0.00899273
0.00108488
0.078275215
0.318603246
0.041882631
0.005676372
0.284864524
0.023827351
0.002547867
0.02977139
0.006407707
0.017108082
0.009890974
0.000381683
0.003835097
6.9713573
Adjacent Benign
0.003219003
0.001646644
0.000233558
0.003923437
0.010351675
0.005823148
0.004188598
0.009445643
0.01896164
0.021296686
0.011776659
0.015881941
0.001803267
0.010873115
0.002328092
0.009572003
0.004639262
0.00541939
0.002863931
0.00493528
0.000603521
0.006148408
0.001956045
0.002432392
0.005548375
0.011064788
0.007105338
0.001458586
0.001255527
0.033083994
0.003188275
0.002237086
0.017344566
0.010567787
0.009524411
0.051208019
0.021646972
0.0057143
0.01545451
0.006528355
0.002519756
0.036468781
0.008558352
0.010822801
Continued
152
Table A. continued
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
0.156077402
0.273344655
0.062292124
0.003210086
0.009824138
0.001427992
0.018770737
0.113455273
0.053755142
0.204774868
0.072782609
0.047490856
0.000303129
1.501904488
0.025448626
0.024108648
0.079367522
1.425510899
0.105448397
0.005033104
0.115073383
0.03889639
0.003287862
0.002261889
0.015817568
0.032213273
0.041040641
0.009544095
0.336204297
0.017677206
0.000324672
0.011954566
0.001773097
0.010771247
0.012527037
0.000569694
1.124034307
0.259493277
0.00173979
0.053190985
0.008855046
0.174041959
0.11382549
0.06740517
0.000758332
0.005657954
0.007794737
0.006178981
0.012843548
0.229469222
0.050202519
0.140910602
0.001250622
0.025862545
0.00052715
0.002960546
1.200033823
0.008153166
0.016621675
0.062571525
0.656514404
0.00904403
0.004790236
0.112902949
0.01301159
0.004468245
0.003816043
0.001620089
0.008049749
0.023990467
0.029998695
0.010044876
0.152454757
0.045638623
0.000894607
0.000151693
0.001693084
0.000173985
0.003367142
0.121675033
0.086076461
0.066609099
0.019498792
0.030810504
0.000163743
0.035227199
0.044840326
0.021428439
0.064043172
0.607524531
0.011247803
0.00698449
0.129196695
0.042972174
0.001008326
0.000699254
0.162341592
0.004104854
0.00036525
0.009155803
0.002599786
0.009548634
0.022876007
0.02228078
0.000165124
0.181180748
0.014120912
0.000309193
0.01539088
0.002959749
0.000144635
0.005495992
0.015571612
0.000769415
0.278904587
0.221867471
0.00092881
0.052300573
0.012598498
0.00012471
0.007871286
0.025333462
0.001368625
0.891192854
0.150235855
0.001507435
0.348553029
0.006302979
0.05389782
0.266531919
0.068708134
0.001112762
0.005058089
0.0005465
0.004656992
0.001493087
0.099270868
0.074940334
0.102862264
0.000303401
0.04124096
0.001892753
0.000264216
0.533652763
0.036020318
0.019036799
0.022874051
0.912583971
0.012385116
0.006123918
0.131683749
0.030975937
0.005605024
0.029521487
0.000255493
0.009069786
0.020156573
0.016353471
0.177014656
0.007388497
0.000780286
0.055732849
0.004140476
0.000249382
0.006107824
0.025478784
1.147945555
0.319546152
0.000970997
0.60279369
0.025774187
0.42119379
0.285545701
0.124172884
0.00101393
0.003654635
0.017652105
0.015822222
0.058034478
0.151546829
0.066453089
0.334647367
0.042358508
0.030029943
0.00016767
5.56391573
0.025029514
0.049623716
0.13764824
5.816894221
0.012075561
0.004343039
0.188996192
0.064792834
0.002936795
0.013385748
0.00018277
0.025886829
0.130639182
0.064907638
0.00596619
0.333619627
0.067239559
0.000264862
0.028583929
0.001218406
0.445348077
0.612935117
0.122161854
0.006873555
0.011068939
0.023057338
0.037806622
0.080209658
0.249867258
0.13410294
0.395013337
0.134387166
0.042803031
0.000496062
0.000743265
3.534118144
0.06061866
0.040917314
0.214746313
4.507791443
0.025820545
0.004452315
0.276350719
0.067463841
0.000800383
0.034633338
0.00454898
0.024622819
0.152129809
0.101747572
0.005911626
0.290619874
0.037292925
0.002796651
0.041453444
0.004212564
0.005416338
0.026786559
0.040930343
0.00797047
1.097127024
0.729152542
0.01062897
0.087067166
0.074910178
0.04677749
0.045465145
0.003997888
1.409226265
0.6203603
0.013378301
0.111896186
0.064522774
Continued
153
Table A. continued
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
0.033429523
0.050694641
0.154609575
0.005116539
0.015226372
0.000670647
0.000724876
0.001066134
0.082889667
0.002931117
0.038637996
0.129790579
0.017640522
0.002257931
0.059722467
0.173840673
0.026687714
0.00240095
0.000658507
0.048511667
0.014991817
0.000680202
0.078728542
1.067937391
0.004390741
0.006851913
0.112882799
0.123233344
0.001727677
0.000319802
0.036249278
0.044961176
0.079926214
0.001398151
0.002906444
0.003114314
0.000862998
0.000576575
0.066300087
0.011214605
0.517754671
0.008451526
0.000347188
0.023213737
0.00045774
0.00777064
0.009808805
0.018034323
0.122808776
0.030451003
0.003569036
0.002381058
0.004683363
0.035653711
0.014088019
0.26796966
0.015601852
0.000911752
0.011188544
0.00040188
0.002639743
0.017715783
0.004860362
0.047948868
0.012556238
0.003699959
0.001678749
0.003224637
0.001603305
0.235941658
0.235761013
0.015572745
0.001203728
0.052692906
0.152248569
0.01075514
0.002549942
0.002686129
0.084603905
0.010984146
0.001497081
0.045011644
1.336976465
0.018793402
0.012375542
0.206795267
0.315547166
0.000786065
0.001015358
0.015768345
0.07850055
0.084224576
0.002010854
0.003582022
0.043072491
0.010867656
0.002987762
0.001218382
0.036995332
0.000781507
0.006278051
0.002029374
1.102588584
0.017214732
0.006063076
0.216273539
0.158971061
0.006192231
0.005560879
0.025803714
0.089600499
0.025135864
0.002868652
0.003458274
0.001136085
0.001544331
0.001728739
0.144733578
0.000462177
0.005989983
0.479720857
0.010951802
0.022409909
0.000990003
0.001681868
0.0377653
0.011763634
0.088025179
0.023499357
0.004052687
0.001132278
0.010789898
0.00961808
0.047561144
0.11851263
0.023321829
0.0026566
0.040712192
0.118730313
0.01581359
0.002515619
0.005805475
0.044760803
0.018714181
0.001215792
0.035357859
1.241384226
0.012338255
0.015875042
0.22336642
0.227245199
0.010242067
0.00311748
0.023672432
0.072949466
0.047759407
0.014636184
0.002605853
0.001619897
0.002664959
0.007064216
0.132227093
0.00046819
0.006822167
0.526207683
0.039678663
0.0002132
0.020842934
0.000746375
0.009283466
0.016374892
0.006406296
0.095504546
0.018558678
0.005374349
0.014883908
0.002446533
0.00401225
0.000221379
0.122494771
0.355137982
0.022313282
0.002562772
0.062722422
0.417790493
0.009152107
0.00459102
0.002438195
0.098270457
0.118832311
0.006702658
0.109963571
1.586315318
0.036454559
0.017239928
0.270976861
0.45856418
0.001507291
0.016005766
0.044246038
0.049220413
0.200802491
0.048358358
0.007809707
0.027114306
0.008930174
0.003303705
0.070429984
0.024340432
0.243503948
0.026571787
0.006172721
0.152216301
0.555116996
0.040999802
0.001730264
0.114530898
0.459355265
0.048140995
0.011318596
0.007479024
0.083749203
0.048184377
0.006046269
0.167638075
2.386303875
0.052427471
0.036817826
0.553785149
0.550764802
0.005174768
0.013241228
0.019377064
0.185451876
0.349498161
0.037962301
0.031701486
0.011153206
0.023446165
0.003278719
0.272459041
0.000408068
0.048454846
0.543002321
0.044878267
0.137626297
0.000552401
0.030928658
0.005185303
0.075776119
0.195066247
0.03085871
0.015999433
0.008987076
0.019285657
0.004369722
0.085129164
0.000462866
0.049109324
0.015497295
0.017302158
0.356558393
0.059512592
0.023107056
0.027016784
0.024446526
0.046171047
Continued
154
Table A. continued
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
0.008739164
0.006196471
0.00207454
0.097172411
0.008598809
0.032143494
0.238603493
0.181171141
0.000558598
0.007268549
0.000658371
0.018058502
0.008240885
0.004080247
0.297343011
0.005299245
0.007123347
0.039444202
0.189617046
0.00087355
0.497798079
0.013458119
0.180422175
4.095998166
0.021707706
0.007691571
0.001020997
0.107210794
0.013931497
0.009027684
0.003284983
1.139689297
0.263884875
0.026633901
0.007426131
0.142593346
0.005906295
0.026532769
0.000544327
0.019397413
0.0102539
0.004515206
0.001690099
0.003724321
0.001013411
0.008826817
0.01807232
0.000549543
0.069783007
0.004633092
0.040467501
0.444582039
0.04262321
0.003961983
0.00308475
0.000250425
0.000778382
0.013162306
0.015266213
0.000438034
0.24129101
0.00660894
0.001871324
0.014298083
0.045936845
0.000214464
0.799643061
0.005413726
0.075547984
1.74546759
0.015252273
0.002094369
0.001314431
0.041991222
0.005608937
0.001421654
0.002320861
0.685260089
0.182771252
0.001685785
0.005707231
0.070352869
0.00176932
0.015560099
0.000401429
0.000438938
0.000796762
0.008536329
0.001991851
0.030947734
0.453059699
0.115565011
0.001154775
0.001278001
0.009368866
0.004720704
0.002495298
0.038550362
0.001918504
0.033263233
0.373060422
0.107269975
0.000310979
0.000639631
0.024332967
0.066524961
0.002561531
0.166420443
0.009382591
0.116268006
0.475669052
0.73587242
0.003765043
0.008641111
0.000847187
0.041929765
0.010562928
0.001203061
0.262356887
0.011725129
0.00155058
0.038965161
0.25367277
0.005640857
1.013671412
0.007984001
0.100902272
5.53811714
0.107896433
0.001304947
0.006133442
0.05457712
0.000388849
0.0012271
0.004636049
0.415676205
0.27665647
0.023479583
0.006498503
0.157445297
0.005884178
0.018389264
0.000208854
0.019252308
0.003656862
0.002232689
0.110027703
0.005306211
0.005808471
0.030449371
0.202323418
0.000440909
0.731949855
0.003251728
0.069749961
6.880177186
0.01228045
0.001843116
0.007089284
0.002822347
0.004346103
0.001967066
0.002775237
0.001732987
0.004667189
0.001696013
0.001716496
0.000540588
0.001366649
0.001290372
0.000496219
0.020109466
0.010758286
0.004554802
0.21278363
0.005740733
0.015049184
0.059391924
0.285322034
0.008943583
1.000941091
0.006032081
0.316475576
6.191369533
0.068796647
0.03170013
0.004690611
0.225873209
0.006043995
0.017358109
0.011147898
1.354844773
0.446570721
0.185966101
0.034337851
0.223581166
0.000374749
0.272845768
0.001535443
0.027820923
0.000853387
0.003181942
0.010153596
0.007657905
0.002335613
0.031980764
0.001429942
0.002029891
0.00870919
0.167266765
0.209902568
0.058080864
0.004705345
0.138599717
0.000159815
0.010548109
0.000145199
0.008428845
0.000178188
0.005808445
0.008587802
0.00138896
0.000699716
0.007784827
0.06114737
0.011229689
0.308155889
0.010075294
0.197394031
0.580944508
0.547239119
0.003647572
0.02614141
0.000339337
0.002886264
0.033714331
0.014924788
0.004502761
0.799173843
0.027134521
0.049274832
0.09970905
0.315722125
0.005461098
1.476407805
0.039974679
0.3840639
7.367462281
0.176803013
0.064013712
0.001887462
0.312754006
0.003970808
0.018082159
0.026583793
2.194505323
0.78599349
0.106532776
0.010997419
0.318789666
0.002932552
0.08897441
0.000902913
0.042569554
0.013578028
0.005308315
0.001787793
0.009410791
0.005021617
Continued
155
Table A. continued
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
0.021638234
0.016779332
0.030739523
0.002905409
0.001671448
0.014037692
0.006637214
0.003558781
0.011101828
1.795608797
0.039524501
0.055726412
0.01259103
0.001893571
0.206092666
0.03223628
0.097557228
0.001043954
0.037751652
0.000773837
0.073799051
0.062018312
0.01350495
0.350543544
0.090088862
0.020562066
0.002762393
0.025557421
0.013666604
0.030973593
0.001421795
0.001432421
0.009199927
0.002553558
0.003707237
0.005988764
3.289936115
0.041433172
0.04804315
0.033482303
0.001223979
0.230141182
0.012095126
0.027971493
0.001386457
0.022216896
0.036187345
0.041251863
0.007849076
0.270729043
0.015340152
0.013229232
0.000297385
0.008678889
0.038613913
0.014778494
0.090631324
0.00102095
0.001484567
0.008301893
0.002007514
0.004372718
0.019042432
0.258181596
0.034394831
0.030020869
0.037368606
0.000421041
0.791047411
0.016349525
0.285186297
0.000729032
0.043250978
0.063324521
0.055842083
0.041818353
0.014434516
0.389454197
0.567170369
0.014924264
0.038797506
0.009389338
0.087728184
0.000386695
0.001344435
0.005957663
0.005042276
0.004119177
0.00640881
2.148871508
0.039717296
0.0252393
0.039328888
0.001382688
0.437211793
0.009695901
0.051885698
0.008696566
0.019221091
0.095289071
0.0058713
0.008632556
0.037275488
0.000953833
0.005444761
0.024686944
2.323744691
0.055565514
0.078389518
0.017826522
0.002858711
0.594908654
0.036549338
0.228247912
0.033478084
0.031866696
0.148039212
0.017762067
0.005327614
0.0353411
0.007174643
0.007732396
0.043652157
1.960181534
0.116587722
0.08321952
0.048511001
0.005407891
0.367686795
0.043044889
0.521268566
0.038542547
0.036599309
0.069319628
0.029906393
0.020664084
0.313538763
0.581581474
0.017117861
0.054823702
0.100154009
0.124162986
0.000248125
0.006167551
0.001585969
0.181389276
0.167537455
0.015430767
0.009333139
0.059573708
0.015381922
0.007160823
0.004727747
0.008793981
0.046272209
0.000418386
0.12692822
0.127060479
0.032895494
0.595157028
0.249313648
0.030476389
0.00432079
0.000533164
0.014161332
0.122309152
0.151275658
0.002027938
0.023381978
0.000821807
0.235983807
0.2948857
0.024634197
0.010166908
0.062262282
0.003542559
0.002066094
0.002053751
0.012559425
0.037434027
0.008449895
0.053077152
0.025349617
0.011705903
0.061321384
0.078235737
0.000571339
0.009413654
0.024106651
0.04891952
0.000329452
0.009162396
0.029574387
0.06485547
0.000385333
0.00822495
0.04990111
0.057243878
0.001134313
0.012185728
0.113815509
0.007708886
0.003676877
0.001544141
0.023621478
0.011458405
0.003291062
0.003515956
0.006798745
0.012963212
0.001442693
0.002957325
0.003236343
0.01855919
0.005951694
0.002320368
0.00153345
0.002960483
0.021293355
0.015180115
0.012380952
0.01248593
0.065761001
0.00042116
0.005718306
0.008925366
0.00534838
0.008073218
0.001581146
0.001235961
0.002885863
0.000510172
0.032601396
0.015330011
0.019541117
0.000414878
0.01205171
0.004419913
0.024262116
0.006201162
0.000445611
0.000448333
0.0046032
0.000186446
0.036130655
0.012016893
0.088011117
0.117032576
0.0403018
0.153215809
0.130991406
0.024979737
0.003626158
0.002211545
Continued
156
Table A. continued
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
0.000655915
7.0593355
0.00138276
0.005043245
7.1759525
0.00039961
0.000268085
0.038575471
0.007785204
7.430182
0.025073562
7.066974
7.09703565
OUP27 (B)
OUP33 (B)
OUP42 (B)
0.321861154
0.011628666
0.034445095
0.00286499
0.083597892
0.070628189
0.000224623
0.001812017
0.07727867
0.005076983
0.01721141
0.013210459
0.019800505
0.007365754
0.003177816
0.003512886
0.007224064
0.00740626
0.056976879
0.017638105
0.004259125
0.119152689
0.295916679
0.211529667
0.002079533
0.000295203
0.02193097
0.000186049
0.045605809
0.237746403
0.094595766
0.189655934
0.016635539
0.125487574
0.04703942
8.0021922
Adjacent Benign
OUP28 (B)
0.08413606
0.008107015
0.0039822
0.008425529
0.001577992
0.015723526
0.005819238
0.048058871
0.012619793
0.065555906
0.014817954
0.004043715
0.212521554
0.276967782
0.156779308
0.001808542
0.003091333
0.027070532
0.011300818
0.06533433
0.305141449
0.045966307
0.397378595
0.070918659
0.054198137
0.000501627
0.000402039
3.922993838
0.019673918
0.059530985
0.084095538
3.509217989
0.035892832
0.010089406
0.19202061
0.03166631
0.004084006
OUP48 (B)
0.000234982
0.043721617
0.004695749
0.005228008
0.000145887
0.013956868
0.001475721
0.027090448
0.005261778
0.005084697
0.002494582
0.002539466
0.013399478
0.000432792
0.198384961
0.075619943
0.081782307
0.001189292
0.004893302
0.011626463
0.037405652
0.121720711
0.052137368
0.592319944
0.105428847
0.018827661
0.000846554
0.000705469
3.365094616
0.036212487
0.020981463
0.079623719
2.809710656
0.010981383
0.008074027
0.099960302
0.025928617
0.001063208
OUP91 (B)
0.000164582
0.037881836
0.009361647
0.006809922
0.014672678
0.041635734
0.002114508
0.011710622
0.008521305
0.002278975
0.0036048
0.006481258
0.003231624
0.404523822
0.061080305
0.028972552
0.004110325
0.01484316
0.011899783
0.039833597
0.029531134
0.403941442
0.059611338
0.01244431
3.227823223
0.024622304
0.034731657
0.033565247
2.701301307
0.014457046
0.00606708
0.084238997
0.016429884
0.001473975
0.117425844
0.003559086
0.048761062
0.00800549
0.061903482
0.026311383
0.850770178
0.077312895
0.064461111
0.033574759
0.047280361
0.0686602
0.168712511
0.109591808
0.025978931
0.382361685
0.059209557
11.79674446
0.051963192
0.131601594
0.106167041
6.696340409
0.090961627
0.01501254
0.078715517
0.128196108
0.005803773
0.000370353
1.651297909
0.115946439
0.068897857
0.160887196
4.346802115
0.019237164
0.018548707
0.163247683
0.101167508
0.001906219
0.010525949
0.019896356
0.005949032
0.003923197
0.005078746
0.00090441
0.233273943
0.134810995
0.09337794
0.001513423
0.004886728
0.065753139
0.034755559
0.065151562
0.044719568
0.307391797
0.072384598
0.015128786
0.000518267
2.252012085
0.053735249
0.023021451
0.063663998
3.804182791
0.023804845
0.010727663
0.095625359
0.066751384
0.004740957
Continued
157
Table A. continued
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
0.015314654
0.000237819
0.013500878
0.102604511
0.084763359
0.000629274
0.309218828
0.020608954
0.000220521
0.024957679
0.016092113
0.004782879
0.000255167
0.025387419
0.06071983
0.006051509
0.779840275
0.83784546
0.002226261
0.108884694
0.095282665
0.003438277
0.168955834
0.294929506
0.03104887
0.000796113
0.077773343
0.365844904
0.018157628
0.004642035
0.008884637
0.077360213
0.078756842
0.002370775
0.102293478
2.216024097
0.028906577
0.012189786
0.313967486
0.40428962
0.028034916
0.003088596
0.045509863
0.073986767
0.22107918
0.029853483
0.002282102
0.002898233
0.017685172
0.002562215
0.124493349
0.41963199
0.00306157
0.094329483
0.027529354
0.00037762
0.154783551
0.227929185
0.029861492
0.000331205
0.029381494
0.206893406
0.011169745
0.104714045
0.00714361
0.057823994
0.025625857
0.008994885
0.131902706
0.028395794
0.000533374
0.006099316
0.067999199
0.029109605
0.008634738
0.110714
0.003922288
0.014539687
0.00132246
0.001610452
0.000597503
0.001205011
0.063626963
0.010662363
0.003722611
0.011745293
1.656945407
0.011604668
0.011882285
0.173418542
0.143096676
0.00558854
0.008384841
0.021765222
0.056522578
0.089114319
0.026907759
0.00448304
0.004436942
0.000204695
0.216903926
0.239425392
0.002911853
0.070551256
0.022930348
0.000962309
0.346808117
0.152352974
0.010025584
0.00121592
0.047628917
0.192343245
0.002391069
0.000196141
0.000572846
0.02803547
0.009916564
0.000243287
0.016270322
1.617585051
0.008562469
0.007685384
0.05710853
0.060107218
0.005825663
0.015713736
0.013035905
0.034670863
0.00408374
0.018799139
0.095296258
0.08391278
0.028996616
0.077957567
0.030303314
0.000527277
0.048682375
0.000946134
0.004221994
0.010784472
0.012888399
0.020968528
0.111881536
1.672631188
0.006143723
0.162735778
0.384194792
0.14476722
0.365027704
0.008893589
0.004037087
0.10624953
1.063596452
0.001568711
0.065445208
0.230666015
0.232877049
1.082989585
0.03655326
0.00596623
0.025112429
0.04265066
0.000374729
0.032162545
0.125734622
0.009255684
0.019632366
0.023525976
0.007100252
0.000627306
0.035800273
0.096918267
0.0280025
0.023877911
0.09198264
0.009475097
0.000727156
0.053107279
0.007038862
0.001558821
0.011201901
0.034225286
0.004869382
0.793870955
0.579853727
0.001031319
0.112613947
0.030445365
0.001052697
0.089292228
0.252951802
0.077070626
0.00345913
0.040860266
0.267694596
0.040712706
0.001944131
0.00659248
0.030932139
0.020745646
0.005118509
0.027331897
1.126158131
0.015758788
0.008038304
0.560911831
0.436634012
0.010806618
0.00081037
0.042394265
0.062635393
0.193212913
0.008821905
0.028025503
0.000680682
0.015091699
0.104093152
0.024250731
0.008541855
0.139425425
0.005815605
0.012241037
0.00072473
0.005442254
0.054579692
0.00991631
0.528221551
0.664410634
0.000712835
0.0792654
0.027402372
0.002080896
0.109907411
0.355640509
0.036737253
0.000291003
0.0639964
0.41292311
0.01670109
0.001203634
0.004228399
0.042160859
0.024832014
0.000501884
0.037173892
0.83118589
0.027933401
0.004625216
0.245182101
0.204870912
0.000745937
0.000807703
0.02670025
0.062626327
0.068271183
0.00515723
Continued
158
Table A. continued
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
0.011710744
0.012992898
0.004923941
0.000718863
0.119689921
0.000234661
0.031089281
0.210736785
0.010665412
0.00042125
0.061210505
0.000256347
0.02497475
0.007124599
0.042273183
0.132515275
0.040269282
0.012811605
0.010489612
0.006999621
0.000473873
0.00782965
0.031671194
0.003025911
0.231623906
0.007699829
0.131239651
0.6120577
0.392152056
0.005301438
0.014227378
0.000151625
0.000558084
0.044483283
0.01844468
0.003320436
0.492637743
0.004301889
0.029827711
0.048395579
0.25833653
0.008228191
1.067870771
0.033527555
0.255282062
4.703462067
0.000449021
0.00315236
0.000762835
0.001660083
0.043784334
0.008062137
0.019444508
0.010697556
0.000749769
0.040409269
0.006282678
0.028983394
0.001176041
0.002953181
0.006288985
0.017146339
0.011324643
0.002353161
0.001111941
0.121668062
0.001666304
0.004839458
0.000265256
0.003175957
0.060761659
0.017324218
0.026621858
0.009474773
0.000182078
0.019807959
0.017328728
0.010171153
0.008380111
0.006608451
0.060264945
0.032199461
0.004804847
0.064671419
0.010908009
0.017214213
0.490124228
0.009668864
0.006653592
0.006743095
0.166105594
0.02143109
0.01495816
0.013312184
0.011451057
0.010814262
0.003305353
0.028904884
0.00132746
0.140235992
0.008899463
0.084854829
0.350467257
0.186860634
0.000484393
0.001181555
0.002814281
0.009418892
0.017285176
0.053524494
0.010986583
0.002590492
0.000643859
0.020678761
0.042956584
0.000680438
0.016081654
0.002040976
0.141192667
0.001471681
0.001509601
0.283879597
0.263269641
0.000333395
0.001280577
0.001669416
0.003325934
0.093089604
0.018059921
0.095748014
0.001253338
0.022770052
0.01088628
0.054559846
0.209116219
0.097962498
0.00591001
0.031469223
0.00422692
0.019808252
0.506095436
0.643156559
0.021902465
0.00329149
0.0006792
0.172730717
0.007242847
0.012476042
0.015876383
0.078302331
0.000691307
0.368490561
0.006555216
0.101079131
2.384878171
0.008314156
0.000616123
0.001073062
0.171225273
0.000641151
0.000687665
0.008424198
0.077371233
0.000310343
0.259500922
0.007164407
0.038226186
1.777765542
0.001383875
0.013183323
0.000821191
0.060741796
0.005008573
0.012590125
0.046986866
0.073212833
0.013556254
0.182511765
0.16130291
0.025767125
0.657466991
0.017621022
0.021759775
0.003236343
0.000278555
0.027306456
0.000264944
0.045892586
0.007954596
0.064617105
0.246809173
0.049614493
0.014571502
0.004651357
0.017671311
0.000850523
0.00161709
0.032485651
0.008911676
0.172687782
0.010514973
0.115810496
0.537428196
0.476374442
0.008131597
0.006393178
0.000233282
0.000465288
0.017967624
0.011183448
0.002687984
0.323705643
0.02227901
0.014437161
0.038942503
0.275926977
0.000831424
0.674776866
0.024979933
0.220654247
5.951620014
0.035988046
0.024778816
0.004778285
0.045206955
0.144175814
0.030865641
0.008452156
0.00018941
0.00160908
0.019621213
0.001200658
0.048816801
0.001499668
0.143819337
0.016087992
0.05080015
0.365881422
0.365849596
0.005681169
0.002498412
0.000280636
0.000249152
0.007758328
0.002291398
0.002336058
0.224219797
0.018257184
0.001411738
0.010778488
0.134609121
0.000742233
0.366588407
0.039653598
0.072754489
3.004357655
Continued
159
Table A. continued
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
0.072216788
0.039647565
0.00406014
0.165458472
0.002896328
0.008818323
0.017574587
1.90050401
0.570812
0.132632898
0.030048975
0.258099557
0.009568285
0.107962865
0.00040942
0.05234393
0.005928826
0.006214304
0.045575682
0.016091996
0.002923861
0.043112576
0.01532531
0.078681325
0.014894015
0.003014402
0.029120594
0.01037688
0.00483927
0.019564548
1.634714926
0.086797637
0.052501377
0.027058498
0.002971626
0.392702332
0.030269435
0.022495256
0.000464215
0.080553862
0.000598119
0.123387664
0.06950117
0.022062706
0.210104515
0.199630432
0.190434275
0.066019456
0.000479102
0.051715716
0.000585291
0.024298466
0.003567121
0.534722233
0.292701404
0.082847161
0.011276828
0.07755317
0.031083937
0.008855369
0.003258583
0.018427388
0.027832283
0.000207173
0.001241969
0.009921532
0.508250653
0.153841866
0.124400791
0.01340049
0.110557597
0.006131148
0.017841928
0.00072871
0.104393086
0.001661952
0.004554355
0.001038774
0.005444088
0.011350838
0.008175902
0.00245088
0.014976456
0.004319816
0.054944774
0.029149846
0.001285655
0.014553047
0.001964958
0.003929107
0.01396628
0.989010381
0.058084703
0.03753091
0.00847426
0.00247575
0.446743012
0.009721766
0.174547073
0.088923656
0.000245461
0.013413198
0.001767721
0.000435066
0.002001654
0.001190568
0.000530116
0.013955886
0.002994118
0.003418628
0.002720049
0.004369301
0.018129005
0.000777434
0.000257331
0.005496657
0.942101933
0.053378888
0.013700383
0.010734204
0.001537659
0.164869229
0.003750032
0.044244489
0.015869979
0.008156783
0.05465162
0.064959826
0.005099657
0.191396794
0.285835511
0.00096346
0.058363353
0.088047879
0.002649976
0.088861547
0.22105382
0.05847618
0.129316061
0.578056481
0.047280738
0.089510974
0.007101194
0.001513805
0.057306153
0.009085524
0.055376008
0.019668917
0.003006313
0.00855908
0.048733693
0.010905762
0.054720316
0.014803005
0.005290135
0.001917303
0.000770693
0.005817255
1.525698475
0.026394833
0.017316442
0.009581966
0.000482912
0.03598269
0.002039085
0.013797755
0.001705795
0.006396992
0.060271232
0.149203974
0.072531544
0.016947384
0.298317469
0.181465717
0.099660451
0.000983115
0.144945296
0.006921418
0.012548232
0.009112556
1.154804728
0.593116709
0.069205496
0.029589998
0.158627567
0.006205621
0.07325771
0.000849568
0.010000916
0.009114754
0.005951004
0.01520112
0.00567379
0.003730473
0.032614593
0.028180781
0.117998949
0.027557903
0.006631148
0.009473156
0.006566469
0.003552945
0.034933655
0.848276452
0.066951023
0.037078056
0.034536769
0.00297898
0.92444021
0.015856096
0.392531114
0.000294875
0.044751254
0.139155846
0.071044162
0.03361564
0.023778218
0.289629994
0.174906695
0.049982663
0.014937821
0.037148418
0.003178783
0.004020759
0.000879958
0.404474756
0.273940707
0.062857494
0.007572391
0.113021102
0.074498752
0.000603338
0.006047498
0.01049447
0.002214173
0.000667747
0.000675161
0.001107651
0.014438904
0.009503203
0.050009087
0.023711744
0.001801872
0.010280506
0.005096544
0.001754567
0.007863891
1.278434786
0.06927519
0.031599785
0.0164059
0.001623585
1.566199583
0.003701896
0.149622295
0.011896261
0.045999853
0.05604345
0.1144591
0.012693529
0.166878334
0.119635509
Continued
160
Table A. continued
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
0.029099871
0.006116846
0.043934987
0.125301281
0.000701899
0.014851918
0.000461169
0.161824457
0.070940562
0.008541343
0.005377037
0.008220296
0.000764528
0.001563587
0.008034253
0.042041926
0.020461469
0.015747756
0.004937966
7.288695
0.011969806
0.002986056
0.000188323
0.00915447
0.06120535
0.03285388
0.008239669
0.002166815
0.104045687
0.102342307
0.002637997
0.001527965
0.054211926
0.015409059
0.01483849
0.005916756
0.0063059
0.059649571
0.005353871
0.000776753
0.014552788
0.00770823
0.002077999
0.00872308
0.084327639
0.006172574
0.00132686
0.009597698
0.171561361
0.144028734
0.009593727
0.002291028
0.066550835
0.008523006
0.001041043
0.004607718
0.001416751
0.019073112
0.007385471
0.02244214
0.023588534
0.003011961
0.00022913
0.000501465
0.000859515
0.003519514
0.385698214
0.015221854
0.000365252
0.102617122
0.029427838
0.000234227
0.002593456
0.00076871
0.000638454
0.000355638
0.000931051
0.02186877
7.2963655
7.3134995
7.9507668
0.030087383
0.000558584
0.01042334
0.037748769
0.126983629
0.002392081
0.015317641
0.002013852
0.071583774
0.058887467
0.034747366
0.019686459
0.00901298
0.001436367
0.003273774
0.004123975
0.011731058
0.015790116
0.001278695
0.00019956
0.013383739
0.045432439
0.034278792
0.004096831
0.120343704
0.120841681
0.003542248
0.002171276
0.063004604
0.010491524
0.003442245
0.002206109
0.001394468
0.023065069
0.012388484
0.002311079
0.004139409
7.22896885
0.007148073
0.004602476
0.011818208
OUP49(B)
0.003450972
0.03991665
0.001943705
0.005586341
OUP101(B)
0.005977055
0.0373892
0.013502889
0.029624311
0.019825245
0.000861823
0.031752422
0.001844438
0.019393392
0.022833961
0.00045796
0.06957549
0.053522895
0.002347539
0.182279026
0.121941531
0.087539926
0.003604225
0.029853645
7.6139735
Adjacent Benign
OUP105 (B)
OUP122 (B)
OUP139 (B)
0.171442693
0.005822275
0.034222971
0.000671359
0.100665297
0.271995421
0.011321189
0.012946377
0.000450217
0.025669176
0.001469486
0.063836407
0.002239283
0.024640883
0.036662622
0.0001661
0.070586582
0.024267584
0.008434637
1.115347113
0.060378816
0.097165285
0.041441454
0.003395118
0.015466166
0.00053108
0.020641819
OUP29(B)
0.002036074
0.059309784
0.002460471
0.015948394
0.000608686
0.020184014
0.038071334
0.042773303
0.007943425
0.014480773
0.017135076
0.01204077
0.073032626
0.02222934
0.000793416
0.273927862
0.122419596
0.085711727
0.000179446
0.142972622
0.00293584
0.046798361
0.004958847
0.050465974
0.052003348
0.005175238
0.833719983
0.134446706
0.167261501
0.002190754
0.003562829
0.017542497
0.000779341
0.14052799
0.113434541
0.101194404
0.004037377
0.031526819
0.003533604
0.008377804
0.016913387
0.009270089
0.026205808
0.003160654
0.000301137
0.004832704
0.269762725
0.083538869
0.000417506
Continued
161
Table A. continued
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
0.008907423
0.014350366
0.008878821
0.058628454
0.139998892
0.021996798
0.863942271
0.126659818
0.012807377
0.000735194
11.41062428
0.074296616
0.143565133
0.137688485
6.061510365
0.079524341
0.006972902
0.106487598
0.099868625
0.004212822
0.072507011
0.0004825
0.012676992
0.08675227
0.05395808
0.009111071
0.177638207
0.038293645
0.000226292
0.026388393
0.001978356
0.001062677
0.065061835
0.012980536
0.289410731
1.854886382
0.00842044
0.198619176
0.194540821
0.004468616
0.137637531
0.635003223
0.006915229
0.004366331
0.03057341
0.009736509
0.139459756
0.018137724
0.050395694
0.829451062
0.059801616
0.007224464
0.00039806
0.00738248
17.49711634
0.112934663
0.178679901
0.075503575
8.819499397
0.032275623
0.022133889
0.041007689
0.248310258
0.004134382
0.12539383
0.000227837
0.017232308
0.065113354
0.046819465
0.027173068
0.079189023
0.031802396
0.000205852
0.006486835
0.007673561
0.054333674
0.162999554
0.038492306
0.181720542
0.053923518
0.024924285
0.000409696
0.014238485
0.002112287
0.03133548
0.080853269
0.041174756
0.315043821
0.065642087
0.032861165
0.022055725
0.015435339
0.017943796
0.081828231
0.145360881
0.032552045
0.407389999
0.022363014
0.006048638
0.000191459
1.841671078
0.032673151
0.059395087
0.058129948
3.085846757
0.011242997
0.008222472
0.106156964
0.033746844
0.002317432
0.002499034
0.000438608
0.026180545
0.072402806
0.021233447
0.00751518
0.157096878
0.031319346
0.000268967
0.013880409
0.001360882
0.001694727
8.281381564
0.014816192
0.034245292
0.011766993
2.973793498
0.0122648
0.02057332
0.128376004
0.03978576
0.001789441
0.036865751
0.019198052
0.000367248
0.000673233
0.710014267
0.032338441
0.026684317
0.104605848
2.586544333
0.017101799
0.014824789
0.126855583
0.040927105
0.000885828
0.00055959
0.000332737
0.010349837
0.044868505
0.030936492
0.017276035
0.137546985
0.02579764
0.00037556
0.014307143
0.003202699
0.003350196
0.005118988
0.02208181
0.010569436
0.062771642
1.719247526
0.010464631
0.288660432
0.365647846
0.002071022
0.097090959
0.504839524
0.005985592
0.004855447
0.007529355
0.012338928
0.003325673
0.454660135
0.52460553
0.004092118
0.108264219
0.030455739
0.000813845
0.03202479
0.161954832
0.02664507
0.001256448
0.002016076
0.006579795
0.003332236
0.457445228
0.532373225
0.012038921
0.360399251
0.048996718
0.000600037
0.065109322
0.240952541
0.026595517
0.016705841
0.023917169
0.033140827
0.340954332
0.07303212
0.081603504
0.038385797
0.017852921
0.012774224
0.000767622
0.00040318
0.580710256
0.074062801
0.053596289
0.148822382
1.789123428
0.020627588
0.005486999
0.1483895
0.073597033
0.001631792
0.004236411
0.00035349
0.049497618
0.028530432
0.01898766
0.02339306
0.137182696
0.030698148
0.001281948
0.002883053
0.004085926
0.001410371
0.024184415
0.001801792
0.538086669
0.90276192
0.004242218
0.304262401
0.059078744
0.000311706
0.098992084
0.855815652
0.009716743
0.013147952
0.014505315
0.011950369
0.307379087
0.370778866
0.005254097
0.229880886
0.009407603
0.003589417
0.11285032
0.119736833
0.04703591
0.026286584
0.062383507
0.044577993
0.00466896
0.133362778
0.020035301
Continued
162
Table A. continued
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
0.001869572
0.151382078
0.849026106
0.022524616
0.00410661
0.059035122
0.212750263
0.009814142
0.212553632
1.580111997
0.078699093
0.007774784
0.148825054
0.118573155
0.002067666
0.022289334
0.048650816
0.012227238
0.11129609
0.047675035
0.00223109
0.031321949
0.002091926
0.000261425
0.023772341
0.009618155
0.039220883
0.022120163
0.466023728
0.024019065
0.005289774
0.108909961
0.086928516
0.035257765
0.001502221
0.034286319
0.000919004
0.067320132
0.077038308
0.079996174
0.008946982
0.100757338
0.123441146
1.000927701
0.012384018
0.000117169
0.00118559
0.057205821
0.653496887
0.004275673
0.179647874
0.804091882
0.049085689
0.002056656
0.048189838
0.032977247
0.005265759
0.139400367
0.015192017
0.004156224
0.071201319
0.110184735
0.001077774
0.046587566
0.000873836
0.000176663
0.007196872
0.009466634
0.023985682
0.041179932
0.000166561
0.327857606
0.000411582
0.00789972
0.00262788
0.036040423
0.014751876
0.044970782
0.00077307
0.010969406
0.009606747
0.022274012
0.106141428
0.077614756
0.011017054
0.055598442
0.001034073
0.038005958
0.201687408
0.00882707
0.003006461
0.00099952
0.020948125
0.029321636
0.00231327
0.053214968
2.806568787
0.023713089
0.011013169
0.190066532
0.17259211
0.002967465
0.026170729
0.046629909
0.105675176
0.003295847
0.003528515
0.005894705
0.000987531
0.002243003
0.062234157
0.011467498
0.0859142
0.007770615
0.021918235
0.000295193
0.00916992
0.009238757
0.058186727
0.10008852
0.013524245
0.010963144
0.017066789
0.017836767
0.009956041
0.009407303
0.007461971
0.002067445
0.07960848
0.001365182
0.067512662
0.270442898
0.014676039
0.000874917
0.00114563
0.070753554
0.036891862
0.005253252
0.049590254
1.726232656
0.023060761
0.00801294
0.201090582
0.221321486
0.010841299
0.001605969
0.043770113
0.05811623
0.076795944
0.013291686
0.005737872
0.00387682
0.001641519
0.000862323
0.115061367
0.000167232
0.015464295
0.045270166
0.013418144
0.000211268
0.049873592
0.00024814
0.007857938
0.013823655
0.050351233
0.065855736
0.01911125
0.004838186
0.003675564
0.012194694
0.015287597
0.007075424
0.038300523
0.007418372
0.237692192
0.000648165
0.15699271
1.250803334
0.010268555
0.00068566
0.031692185
0.097332061
0.032439759
0.001824824
0.006629864
0.025069647
0.006024885
0.010791378
0.085211766
1.829027757
0.03865002
0.009642236
0.17273316
0.136246821
0.002119046
0.016497771
0.033140897
0.041400528
0.05727028
0.046775665
0.00987869
0.002493754
0.00455792
0.000912428
0.095064564
0.138175134
0.014074963
0.000616116
0.018958044
1.874928941
0.034235346
0.004853733
0.198988908
0.223315906
0.006357183
0.000713527
0.027355418
0.03691949
0.116529725
0.000967633
0.005730525
0.016141936
0.00225232
0.002025076
0.103355704
0.016372018
0.033048073
0.059485216
0.006790775
0.119431344
0.006969916
0.513378566
0.024421607
0.000453244
0.012075296
0.005668697
0.039522621
0.122870154
0.009163173
0.006666998
0.000363235
0.008528321
0.046768417
0.003014966
0.008645337
0.016842685
0.064803901
0.040557779
0.006287782
0.013946964
0.00483984
0.009191066
0.036898819
0.066369259
0.010369003
0.073684295
0.018223267
0.058844583
Continued
163
Table A. continued
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
0.008979481
0.05051926
0.633710937
0.935706071
0.007270467
0.000724496
0.004047088
0.044330218
0.294187381
0.446803926
0.000225482
0.00039633
0.000778864
0.006696026
0.01589906
0.001023204
0.20080002
0.00075266
0.020158999
0.004148833
0.128289662
0.011877079
0.490659235
0.162976439
0.073831169
2.224986094
0.00763465
0.010965055
0.002958968
0.009701276
0.000168363
0.091912779
0.00602119
0.016142438
0.020908049
0.081209976
0.010301322
0.057050695
0.052733051
0.028130232
0.61231578
0.007354512
0.005957201
0.015467586
0.000824276
0.001280679
0.004187299
0.11420006
0.167179727
0.607649507
0.038917917
0.10280425
0.006677625
0.044542113
0.000519976
0.023693782
0.002019892
0.002382353
0.045363486
0.003004928
0.002536158
0.02191821
0.00089422
0.028004798
0.039358194
0.009911398
0.000206456
0.00361721
0.001000292
0.070357609
0.133986659
2.227095262
0.051476482
0.031914686
0.008058862
0.00181394
0.084363501
0.016415719
0.004243432
0.206668765
0.088710659
0.005514143
0.02538591
0.000193964
0.007496133
0.03021299
0.001348589
0.072557946
0.645035255
0.303885116
0.001157761
0.000844523
0.00016077
0.000556995
0.011764676
0.002605877
0.001232811
0.176476797
0.025502269
0.005548917
0.012660226
0.115599288
0.003112217
0.735557954
0.0281056
0.099536152
3.024940823
0.072845033
0.016358887
0.000322681
0.064307638
0.000561284
0.010987957
0.001682885
0.437976516
0.241419969
0.029085722
0.00749061
0.120174351
0.000140962
0.129891274
0.000379363
0.015582331
0.007952686
0.003798587
0.00234579
0.002082103
0.000849194
0.028971344
0.001173404
0.02124658
0.000914754
0.004340321
0.050761004
0.527134645
0.462042805
0.018903162
0.001944489
0.024988808
0.000346507
0.000836408
0.274802383
0.005256543
0.018756903
0.006458653
0.174571254
0.001372084
1.05119143
0.013863914
0.10838026
2.167233981
0.058596423
0.006783925
0.000279717
0.076332641
0.003656465
0.005991716
0.001433102
0.363063529
0.284634791
0.052035947
0.014536648
0.117618542
0.013605591
0.088193089
0.003348429
0.01008886
0.008569094
0.005317768
0.010829076
0.005599045
0.000363298
0.068689335
0.032340312
0.039260692
0.03120901
0.000263908
0.03901425
0.551639041
0.469631951
0.000410903
0.000759956
0.000644722
0.000542173
0.012608168
0.012174915
0.001864715
0.19269873
0.004501955
0.004827768
0.011279579
0.232768779
0.002911026
0.879916463
0.009744109
0.09472772
3.167045226
0.021555607
0.000252496
0.038022204
0.000804026
0.005113015
0.009417615
0.212747999
0.117382844
0.076748479
0.031619798
0.102636798
0.028699979
0.018426367
0.000820813
0.02302267
0.008980708
0.000525515
0.048879471
0.000902771
0.002199216
0.060831356
0.006783403
0.06252084
0.025459837
0.000497427
0.108419895
0.385112116
0.309301111
0.000914804
0.004218657
0.000392073
0.014965024
0.005743996
0.003679046
0.000584266
0.318727282
0.008737234
0.004313703
0.027315363
0.190387929
0.001698491
2.030860887
0.032226547
0.142055433
1.844070637
0.059740906
0.03293596
0.000554647
0.061313812
0.002872373
0.028726488
0.010745176
0.614282172
0.391738932
0.016447481
0.012966522
0.158354846
0.001628544
0.342708984
0.000783614
0.003839183
0.00027338
0.001328607
0.001251769
0.007808702
0.002855262
0.057696432
0.096706483
0.052699932
0.00122564
Continued
164
Table A. continued
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
0.000438643
0.009215929
0.000538611
0.001196595
0.010573108
2.529263454
0.084757733
0.027382924
0.023730673
0.001163984
0.094916935
0.011098298
0.017249189
0.000714167
0.009655227
0.022085623
0.063598622
0.219196827
0.016094274
0.052473209
0.131372418
0.026527432
0.017411313
0.005919593
0.015298408
0.207743295
0.015295969
0.000743334
0.006770132
0.00268758
0.000598
0.004776736
2.614628126
0.161079943
0.020772034
0.008489088
0.000345657
0.023315615
0.001304801
0.016156329
0.005257783
0.011671912
0.003777932
0.023829686
0.19207162
0.019453031
0.01913323
0.055734377
0.008025018
0.003441667
0.019300075
0.022314592
0.353701752
0.004654935
0.007286965
0.001586066
0.018845107
0.684268206
0.061988131
0.001222423
0.215938115
0.019415953
0.001494169
0.001797466
0.00187283
0.003091468
0.004722377
0.079727421
0.005968921
7.79091525
0.000550085
0.01302563
0.005115213
0.001447207
0.012614514
1.53730766
0.056647532
0.032597009
0.018331256
0.000807971
0.376005174
0.004215824
0.043577309
0.000672328
0.013385751
0.043793587
0.071011349
0.095976926
0.008519163
0.673656907
0.187011633
0.014664096
0.00239683
0.01354319
0.012038796
0.003459445
0.007403522
0.538865609
0.053255688
0.029904552
0.004664147
0.004226001
0.368961815
0.002236816
0.1404588
0.001290817
0.021223155
0.03686966
0.326150426
0.07959692
0.022949578
0.139146056
1.702067697
0.019732772
0.01419365
0.004895244
0.005215133
0.00065878
0.012796072
0.96146135
0.037628883
0.000900602
0.423991066
0.097228783
0.010494316
0.047575427
0.049514965
0.001488946
0.00493249
0.000329356
0.079823338
0.103764864
0.007205174
0.002548353
0.029022275
0.003817961
0.017487875
0.000543504
0.002343918
0.003751125
0.005644862
0.003483109
0.00114528
0.001345595
0.068436096
0.003859785
6.9697087
0.004550325
0.016782389
0.009423116
0.012206081
0.002335283
0.001933843
7.26284335
7.50819785
0.012472072
0.017943094
0.004563745
0.001136237
0.012559214
0.824102944
0.007384009
0.042127072
0.006476886
0.000790603
0.058289162
0.010305098
0.00454517
0.00166151
0.023260742
0.004448112
0.003239474
0.018312549
2.33561409
0.062183512
0.030508032
0.010704524
0.00441383
0.108346685
0.009432601
0.194754412
0.004854431
0.012337565
0.282321806
0.061918742
0.023347518
0.078091264
1.720500092
0.0191621
0.008290518
0.014204156
0.052496768
0.441632858
0.064557227
0.010823815
0.363043523
0.966097981
0.031629782
0.001663394
0.068266775
0.033780428
0.008909545
0.029479012
0.006754358
0.004468675
0.076721776
0.093991064
0.006368867
0.002219935
0.028653862
0.017182055
0.010241788
0.065889938
0.001786016
0.003549328
0.005718092
0.000508724
0.000321918
0.00289563
0.000370038
0.011444339
0.003845286
0.005535466
7.5580864
0.074734641
0.036412155
0.000276836
0.015911779
0.000411191
0.054882401
0.132299583
0.01837233
0.003510605
0.017189929
0.025984398
0.000776632
0.001972611
0.005342967
0.00091871
0.004703813
0.004210295
0.00518589
7.96171685
Continued
165
Table A. continued
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
Adjacent
Benign
1050005B1(B)
0.001423629
0.070348517
0.003195853
0.00973822
0.000310178
0.025884968
Pancreatic Tumor
0.025027939
0.017535353
0.002811673
0.098096184
0.008814753
0.008383578
0.133564112
0.122060755
0.098047205
0.000285954
0.000925525
0.003139291
0.037206199
0.019114984
0.139145516
0.044288143
0.449438718
0.026767641
0.008297876
OUP2 (T)
0.000594958
0.41475642
0.034800063
0.042247981
0.00149755
0.110129259
0.001361318
0.156129409
0.006048117
0.066040288
0.066659103
0.000108908
0.101109623
0.021169709
0.008165377
1.379062383
0.047504211
0.032441216
0.000264532
0.073408464
0.025435173
0.084688725
0.270849479
0.025521151
0.027135856
0.978574246
0.029440025
0.000633804
0.000326171
3.32085175
0.032683206
0.017375741
0.02561225
1.6222974
0.05853855
0.004259835
0.17143889
0.021581865
0.002795153
0.018200108
0.000174797
33.03194965
0.056601564
0.170334458
0.200593216
14.4739142
0.095042969
0.028838586
0.021161867
0.159556105
0.004105197
0.090071327
0.000776441
20.00060754
0.002437109
0.093003635
0.009025818
2.232703103
0.033293213
0.0079225
0.026445186
0.082599589
0.00126089
0.006956831
0.000523797
24.40019157
0.042205024
0.092513266
0.084612145
6.045809702
0.031330619
0.007713646
0.087311958
0.077883205
0.012541694
0.040700027
0.000728277
49.81759151
0.015249004
0.061500134
0.042366254
15.82213487
0.073565893
0.010445725
0.026206434
0.110975943
0.006013315
0.032580722
0.031635618
0.007613352
0.035067565
0.11675353
0.059259287
0.004727934
0.663102854
0.060079352
0.001145669
0.000299944
0.000318034
21.54238488
0.011769718
0.117316071
0.056902706
4.468922635
0.028544064
0.023549489
0.088677602
0.032486668
0.002775389
0.041813817
0.020627751
0.050257625
0.01895925
0.143950801
0.004099504
0.054403581
0.022583025
0.0789416
0.006508866
0.1647504
0.034690695
0.077281777
0.045191064
OUP14 (T)
OUP16 (T)
0.012636642
0.008587057
0.023812492
0.08842511
0.020283338
0.003290477
0.577999808
0.001385299
0.000303771
0.040201233
0.003416149
0.032628106
0.039947017
0.000213749
0.087032501
0.019440883
0.017088038
2.40610578
0.049903111
0.025395674
0.000786932
0.017514924
0.042420475
0.052495318
0.133920068
0.095239877
0.014054209
0.389764357
0.026498657
0.011387103
OUP20 (T)
0.000654234
0.273395304
0.01200169
0.031384942
0.000723875
0.086463669
0.002509369
0.109517959
0.002539039
0.058961064
0.081398858
0.017297908
0.093526718
0.013064094
0.002281971
1.68836856
0.034224989
0.040686271
0.00059768
0.032294658
0.051521356
0.100748437
0.32799219
0.067542639
0.014257777
1.537229289
0.007963914
0.001855173
0.054388235
0.005204295
0.003221621
0.000284694
0.01759205
0.001234311
0.040443185
0.153671717
0.014361603
0.031400744
0.000326848
0.029142018
0.016596541
0.020771129
0.005656477
0.076408498
0.023474633
9.73217E-05
0.259782278
0.026638662
0.01455807
OUP28 (T)
0.069215121
0.019794612
0.020265373
0.000183141
0.015816548
0.008588948
0.003217479
0.016901927
0.076358103
0.000274362
0.024111665
0.027358825
0.005971564
0.098712498
0.044998464
0.036625051
Continued
166
Table A. continued
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
0.023641384
0.003100896
0.202680377
0.040828003
0.080363584
0.014624886
0.020485085
0.02452186
0.00929344
0.003271975
0.022983048
0.010487797
0.011671583
0.012337326
0.079666145
0.036083837
0.036099179
0.001742753
0.055252257
0.000234882
0.018020563
0.001279748
0.029261328
0.000336393
0.000482094
0.001097092
0.025100185
0.006630502
0.82492526
0.864319922
0.001502566
0.287747903
0.007726843
0.000574251
0.053282952
1.041487713
0.008822458
0.001913151
0.154871472
1.246659797
0.0126535
0.001916867
0.001143311
0.038220639
0.011968127
0.054086439
1.989325314
0.013021101
0.273889473
1.045013601
0.02132425
0.00339042
0.006943558
0.073632491
1.491380951
0.068899357
0.003559303
0.165441299
0.158091677
0.005957265
0.029254763
0.028256769
0.083809252
0.015335477
0.006577668
0.002986287
0.001205778
0.000732259
0.094145691
0.011237784
0.042430879
0.039026445
0.000247156
0.073340269
0.000798177
0.033005415
0.000267971
0.068590571
0.071635999
0.018140224
0.000276005
0.00453689
0.114554316
1.142245073
0.000253703
0.000537272
0.028757148
0.002042306
0.052680813
0.710710747
0.002561091
0.166356532
0.184805413
0.000114148
0.052932108
0.640659155
0.001499894
0.377205919
2.014763806
0.000602809
0.331988672
1.058910326
0.006769328
0.011517262
0.013679393
0.067317671
0.726886527
0.013732504
0.196485066
0.550541632
0.000603556
0.074149644
0.606960724
0.002588451
0.0010251
0.127712361
1.266147138
0.004133766
0.008766206
0.103826759
0.436484467
0.028819242
0.406998031
0.369918721
0.068079215
0.000823473
0.019140857
0.015114051
0.003137524
0.039175004
0.004488091
0.00455372
0.027093202
0.021934912
0.074539927
0.114399228
0.007247988
0.000679315
0.032821305
0.048809251
0.000857125
0.062798914
0.344945159
0.033367651
0.001592361
0.010305594
0.013109486
0.006230894
0.005173848
0.012952603
0.001958075
0.015784528
0.013030714
0.00866846
0.010742327
0.002720449
0.00065052
0.000866019
0.090736421
0.362610491
0.030752587
0.450461768
1.547889054
0.022981505
0.007884708
0.028149685
0.046471969
0.008432719
0.043977414
0.012135241
0.002333041
0.073104426
0.016963652
0.000924352
0.088190225
0.00151674
0.000227073
0.03514242
0.027287752
0.053692127
2.311994345
0.016380473
0.44922352
0.850119978
0.002618999
0.091755781
1.089584714
0.001859081
0.403460228
2.079282626
0.00010351
0.000366962
0.042088053
0.016018741
0.053018129
1.287634886
0.009978862
0.219059482
0.260610221
0.000506199
0.054819446
0.453651731
0.00510165
0.000257802
0.130109352
1.014989336
0.000988227
0.011991408
0.131816454
0.333609984
0.00265846
0.472090831
0.322749411
0.129549008
0.002342978
0.009116937
0.023445248
0.004700242
0.027373436
0.006477363
0.002116244
0.069236825
0.014889136
0.014142308
0.034812322
0.06091669
0.001188417
0.007437836
0.080511354
0.086095585
0.009407536
0.17547502
1.650171614
0.023501148
0.004101107
0.019040459
0.017381827
0.000470127
0.00887761
0.007122044
0.00247161
0.024672634
0.016643487
0.021576618
0.005343529
0.001017882
0.002637915
Continued
167
Table A. continued
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
0.000162007
0.008248353
0.055352156
0.017013035
0.000170108
0.01914608
0.000508526
0.018011839
0.003418715
0.029661088
0.028928239
0.0221614
0.017515164
0.003047563
0.00310781
0.000790577
0.078521134
0.073427858
0.005278691
0.032742625
0.080126197
0.495805204
0.382627327
0.002138727
0.000377317
0.010704998
0.001189316
0.000284146
0.231352296
0.007635185
0.0206613
0.00777359
0.315556625
0.023011643
0.744604283
0.008845125
0.086970342
4.280903514
0.02990711
0.001475001
0.00034598
0.053025195
0.000773773
0.018631275
0.002938322
0.043010649
0.281342658
0.004491567
0.001012706
0.061098498
0.009460841
0.014447636
0.000530026
0.059223363
0.020732117
0.041700015
0.127762311
0.529327025
0.002931564
0.399783329
0.038885544
0.012232056
0.103222202
0.52261003
0.007645713
0.000266272
0.024622861
0.00609675
0.007366368
0.222707758
0.045423706
0.014404249
0.043923969
0.02069062
0.178786997
0.053776707
0.004857104
0.002795896
0.000353196
0.024406858
0.006623013
0.043236136
0.000327711
0.050788509
0.001033817
0.001944396
0.001217379
0.007895842
0.017992356
0.003151417
0.022734395
0.021598388
0.008057877
0.035422981
0.069205256
0.000324859
0.428272195
0.0221167
0.003025155
0.146215881
0.119684059
0.000152959
0.000284233
0.000425292
0.000235521
0.002080237
0.02819055
0.031358327
0.003963594
0.432932622
0.011730025
0.000639717
0.092822085
0.005639766
0.021915986
0.226108153
0.070716879
0.000135777
0.004901797
0.002823991
0.000606239
0.008700389
0.019342145
0.04795525
0.000218607
0.413330454
0.012734621
0.006219485
0.076879532
0.009479696
0.002573135
0.027829997
0.011829407
0.031936844
0.00232387
0.012827916
0.008297029
0.059609954
0.14405709
0.06624044
0.001868653
0.021532626
0.003437581
0.00065437
0.028425605
0.013594815
0.01247748
0.000460497
0.07522262
0.011625257
0.081907244
0.139463826
0.228176065
0.002983088
0.452498956
0.017430882
0.013686699
0.116697282
0.220727053
0.00011883
0.000777692
0.013648456
0.422300122
0.303578509
0.000362888
0.005407178
0.00064955
0.067335008
0.007436588
0.035846264
0.028887248
0.034968096
0.001968135
0.320494959
0.065001646
0.027048616
0.419195481
0.011251862
0.009749366
0.328809838
0.000183643
0.000163171
0.02016257
0.000189386
0.029055626
0.006898141
0.004984437
0.180595502
0.028799845
0.001184933
0.00765696
0.022967424
0.01322923
0.225827075
0.042175833
0.000144547
0.000404506
0.00473756
0.012370112
0.000498704
0.022307553
0.021121975
0.062566862
0.254115022
0.000452192
0.00791107
0.017861903
0.024331137
0.025761628
0.003200594
0.019393084
0.003217185
0.014092766
0.0821331
0.105751576
0.009558584
0.084079685
0.012374033
0.010320249
0.463699016
0.377130195
0.000320542
0.00169449
0.00362276
0.001433465
0.073685092
0.01124891
0.008691706
0.130427507
0.080224103
0.00259598
0.217100442
0.033399182
0.029196456
0.526664663
0.007332864
0.004377857
0.003819462
0.00234449
Continued
168
Table A. continued
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
0.001984982
0.002603413
0.166658906
0.13028716
0.024943873
0.011435187
0.119638764
0.04486903
0.015583988
0.008193751
0.002966792
0.001172217
0.031372098
0.000281751
0.000359368
0.058444166
0.009403023
0.042670322
0.022523664
0.009143792
0.016169275
0.01321861
0.000685746
0.010840314
0.88907148
0.017313423
0.02337608
0.006190612
0.00186242
0.076486732
0.005196489
0.007967384
0.00111324
0.007868977
0.013068885
0.344605903
0.072734433
0.018975344
0.063969425
0.813830254
0.022147886
0.007063752
0.020032424
0.113925864
0.029213565
0.963572563
0.200780591
0.009174687
0.004663094
0.004792052
0.300640801
0.00259582
0.000132585
0.073490329
0.020685444
0.001212042
0.074824016
0.002735499
0.002600696
0.087728549
0.016078573
0.001778454
0.001064876
0.001226127
0.419966577
0.021780259
0.106369277
0.014729895
0.017236915
0.009870688
0.0011552
0.000193888
0.037268948
0.003897984
0.000299487
0.034905034
0.001332613
0.000286561
0.026717159
0.001492529
0.001867947
0.01676406
0.011714636
0.00455068
0.001301286
0.006055577
1.522499463
0.035881779
0.010188664
0.013532055
0.001027406
0.009429802
0.014491364
0.028540427
0.004623993
0.855360754
0.002077474
0.017434683
0.004005924
0.022216744
0.000640083
0.000175346
0.04340805
0.092358812
0.011040363
0.011244824
0.016382041
0.003786216
0.003605343
0.004241377
0.086379048
0.247468057
0.008843126
0.003089068
0.031585106
0.005055518
0.008037384
0.010289472
0.005407072
0.274228842
0.004137039
0.001816003
0.00186289
0.066135006
0.009615127
0.058884534
0.171086588
0.686635365
0.062404054
0.070371475
0.006479021
0.002476852
0.103573804
0.001936894
0.060384562
0.011253941
0.000341708
0.013940847
0.000268309
0.000718676
0.030743369
0.010732296
0.000747311
0.00506651
6.581710496
0.119722986
0.011538789
0.008328163
0.000284791
0.006837943
0.001070896
0.00131094
0.000267524
0.006128425
0.001089081
0.048272658
0.209861593
0.003878336
0.006105734
0.077717029
0.016090903
0.007813283
0.01557411
0.021170447
0.390578535
0.015136997
0.010792376
0.127703801
0.05940894
0.538302626
0.125496442
0.0179198
0.004399442
0.000632454
0.104541949
0.001079005
0.004598896
0.087033545
0.035167751
0.003150087
0.070294707
0.015296086
0.000187316
0.102489099
0.012898931
0.003238623
0.000354531
0.000296246
0.003109003
3.270910693
0.090518116
0.0023143
0.015322955
0.000108019
0.012402273
0.009896941
0.006459387
0.004491249
0.0066515
0.11314752
0.384719102
0.011063149
0.009219951
0.023651449
0.004675649
0.002367325
0.001370014
0.006108604
0.33460731
0.002018393
0.05291181
0.085007537
0.105167152
0.019430961
0.057123951
0.013177893
0.016206581
0.000336781
0.117272209
0.009428942
0.005858608
0.067769788
0.011913586
0.001435801
0.043150769
0.000389812
0.008094348
0.061407453
0.00770312
0.01810689
0.004456342
0.001801801
0.005894559
3.390836141
0.010950485
0.007463596
0.005070055
0.000235086
0.027921612
0.002220484
0.003468454
0.005335516
0.077121535
0.087591007
0.003529608
0.014441967
0.314494691
0.017377039
0.003034458
0.01612779
0.073458364
Continued
169
Table A. continued
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
0.039729524
0.000673832
0.005368888
0.040970607
0.001813638
0.02209352
0.007505463
0.003430427
0.015131059
0.016315537
0.007414514
0.000352964
0.016226828
0.0117664
0.000496482
0.001290493
0.02463161
0.661370554
0.008789405
0.001279374
0.001141035
0.02256787
0.880770815
0.015128446
0.000334934
0.456266162
0.136483132
0.016912941
0.001289678
0.014556662
1.343314274
0.010797659
0.000669631
0.641024263
0.186616295
0.001103472
0.268418445
0.054731576
0.001702052
0.000720987
0.034556344
0.074945877
0.000569931
0.002082651
0.036440029
0.008789674
0.016307829
0.239373038
0.005941476
0.00084637
0.085209292
0.014466744
0.002117621
0.002635481
0.000204873
0.014706965
0.005866872
0.002357025
0.005784848
7.43896825
0.004880133
0.000890139
0.000407181
0.023802816
0.000871883
0.000973482
0.0008852
0.000921405
0.001569394
0.000402593
0.000818558
0.089123872
0.004630251
6.87257765
0.001140709
0.000836558
0.008895522
0.002615099
6.64977285
0.006732409
0.002009754
0.087598475
0.007988618
7.778018
0.001049863
0.001158201
0.052309322
0.006540982
6.7326428
0.000696487
0.002887534
0.022706963
0.005011067
7.58807
OUP31 (T)
OUP33 (T)
OUP36 (T)
0.251181144
0.021109403
0.046381188
0.002815045
0.135711565
0.00153489
0.179328609
0.005949953
0.075882266
0.397074417
0.072780018
0.026275596
0.02146025
0.155384911
0.002532193
0.286323367
0.014587938
0.096861075
0.189752108
0.014223544
0.090368095
0.069480253
0.03714613
0.001527224
0.063951266
0.001628424
0.011340886
0.023663811
0.001969135
0.040501488
0.026008134
0.005312526
0.422214116
0.061331146
0.103766396
0.000946667
0.00717817
0.02058099
0.017516583
0.077597782
0.079422122
Pancreatic Tumor
OUP48 (T)
0.174871619
0.002451198
0.017043537
0.000618048
0.020703838
0.002047996
0.053401518
0.004326124
0.011708265
0.019014938
0.043985126
0.017389926
0.004963586
0.837572842
0.053929354
0.048408898
0.003965822
0.013663601
0.031978056
0.050341031
0.039068768
OUP91 (T)
0.002375689
0.062582913
0.009024262
0.007846468
0.000456827
0.037607263
0.000771036
0.065145445
0.003916182
0.018601015
0.070566468
OUP97 (T)
0.000251654
0.140469831
0.009691037
0.018585402
0.000594125
0.027132264
0.018191416
0.0053618
0.086214792
0.012899118
0.010016457
0.032333783
0.007968678
0.000326805
0.006778899
0.0095409
0.020280234
0.076212007
0.023005293
0.126406427
0.054032405
0.011184567
0.059576068
0.011599932
0.072104855
0.031493474
0.018514376
0.048217636
0.151176858
0.050641299
0.000454842
0.068748096
0.023399241
0.004264282
0.336669532
0.078948659
0.036210178
0.000142562
0.05793922
0.041288693
0.087076943
0.245329235
0.049920911
0.219981837
0.080194845
0.134006819
0.027032548
0.035979572
0.057527609
0.414347413
0.061398632
Continued
170
Table A. continued
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
0.004092378
0.404686203
0.039188538
0.008293027
0.023879809
0.578040555
0.037596238
0.001173528
0.002761863
0.32674311
0.003551665
0.005369914
0.018091895
0.968630489
0.032937145
0.003356515
0.032053845
0.595679914
0.10991565
0.01240839
0.002685945
20.14473217
0.00192182
0.096394305
0.032034121
7.782349215
0.02162083
0.015720541
0.055386885
0.068466198
0.002243287
0.021734334
0.040519272
0.598454334
0.036859864
0.00386905
0.000520497
0.003106072
33.46846708
0.050022417
0.170793748
0.156090709
12.63394305
0.055577377
0.029696435
0.063960036
0.209648741
0.003752732
0.094951465
0.007016487
8.915153074
0.033302564
0.082847448
0.07670506
4.696952713
0.008971367
0.003637923
0.041885322
0.049458852
0.002300939
0.076721199
0.000269524
9.29252471
0.025851963
0.041920177
0.019845003
2.766813919
0.022043867
0.001550269
0.02917748
0.053785743
0.000167934
0.06576351
60.29188037
0.011647941
0.168910079
0.050648236
11.6705797
0.098841747
0.03440193
0.097817427
0.21286399
0.005140997
0.116818391
6.203911986
0.017909529
0.043063674
0.150752749
5.325519328
0.025888204
0.01031878
0.078311458
0.062765353
0.003519445
0.03655622
0.01117558
0.039549755
0.01622249
0.007653008
0.029271907
0.02714509
0.006586796
0.025803912
0.028214794
0.004551354
0.017409935
0.023998011
0.013026124
0.078978798
0.008191738
0.008924195
0.041140274
0.025903758
0.035515007
0.236727968
0.059599357
0.049774584
0.090789173
0.043004593
0.01795595
0.081111671
0.081563038
0.028234857
0.041777608
0.001007995
0.107283422
0.000855576
0.101459884
0.048055315
0.003852279
0.085745889
0.000907242
0.018998169
0.133680063
0.042306869
0.015872132
0.108782751
0.077554845
0.000331804
0.021213695
0.001068435
0.012109395
0.000462068
0.077782549
0.011507305
0.095742303
3.424253049
0.007040794
0.252083102
1.021568057
0.001298185
0.212174961
1.293323174
0.008903716
0.00444817
0.002005769
0.024975722
0.000876822
0.226503181
1.019385487
0.005683736
0.16137866
0.103461271
0.000910355
0.225216935
0.372128997
0.010205921
0.574997393
3.124857322
0.002244292
0.110564046
0.612717609
0.0035056
0.00534726
0.001848427
0.019226108
0.0581947
1.136349063
0.009191083
0.164961478
0.308345152
0.000199618
0.032801124
0.275715555
0.00590601
0.003122135
0.049369781
0.480582516
0.005455694
0.000344241
0.001536199
0.003740904
0.023237564
0.654748892
0.003225301
0.085089511
0.273155357
0.000622726
0.04497843
0.263741156
0.001850064
0.028375964
0.004621919
0.065071279
1.268561725
0.002005711
0.137374413
0.431133693
0.048809566
0.611026019
0.004099411
0.064434208
0.612008298
0.001608269
0.191940321
1.169745533
0.00298808
0.001600195
0.028475097
0.003513477
0.106536934
2.324841835
0.012794181
0.271714426
0.452482866
0.000419343
0.104415556
0.976355921
0.004145807
0.324217433
1.792514848
0.0040745
0.000526904
Continued
171
Table A. continued
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
0.001364448
0.017368471
0.317002049
0.00808599
0.115181472
1.33315966
0.013267015
0.003905234
0.026788908
0.024839614
0.002289573
0.019150244
0.001381522
0.006419293
0.032487692
0.026150876
0.008201802
0.056375439
0.001365421
0.004952929
0.025818663
0.24086449
0.001639445
0.252049696
0.754465648
0.030986103
0.00192626
0.002982398
0.011434365
0.003923339
0.055238491
0.174914491
0.012867305
0.24751
0.908663932
0.034711132
0.00411876
0.015706094
0.013705737
0.022916613
0.033408734
0.002147493
0.031911
0.003103367
0.002568828
0.030632685
0.000463725
0.003172905
0.004768797
0.007834352
0.004321917
0.001268783
0.037249767
0.01236813
0.023474548
0.040394249
0.003589698
0.00116473
0.00459128
0.006231754
0.012956219
0.016016332
0.003402831
0.022137884
0.019204053
0.161045982
0.000688781
0.050396701
0.003982496
0.031666255
0.020191621
0.020697618
0.00255306
0.016226533
0.002979602
0.013885752
0.050208679
0.042972501
0.001364315
0.04543921
0.006139635
0.014254553
0.330303708
0.34728658
0.001826336
0.002576764
0.009158904
0.007242094
0.038570593
0.001736121
0.005885655
0.002101668
0.031618344
0.097400138
0.040023485
0.001786362
0.047703308
0.001891321
0.001125258
0.181212342
0.12979868
0.009246232
0.011626311
0.032429256
0.017091676
0.069283457
0.003327388
0.009561288
0.001939047
0.015597809
0.026685756
0.02834126
0.004902219
0.020613999
0.019633997
0.029901149
0.08282248
0.229723558
0.002429174
0.206746558
0.005095243
0.00617167
0.317220568
0.229974008
0.006272037
0.002345384
0.096971833
0.181022015
0.143317389
0.53615055
0.080185605
0.00227602
0.023302605
0.028605107
0.004774959
0.009723336
0.007171284
0.003154369
0.046176322
0.031493918
0.121218649
0.027810733
0.003332088
0.000227122
0.005706983
0.093411943
0.272093768
0.025264261
0.682134114
0.7704164
0.114472528
0.002182065
0.026883025
0.038794658
0.012460362
0.015585846
0.019136792
0.014693077
0.051722177
0.017255437
0.015258514
0.037016
0.00189424
0.000793388
0.003475921
0.005372581
0.01949134
0.050116677
0.024837001
0.029117793
0.104210772
0.570052647
0.002044179
0.009450898
0.002951064
0.174367983
0.019770266
0.014354223
0.001588551
0.067066474
0.02159845
0.061843812
0.1228496
0.270278483
0.005062249
0.505949595
0.011320548
0.009820942
0.248436331
0.703396991
0.395361781
0.00084399
0.008325547
0.003350569
0.05535254
0.022811305
0.121849042
0.002996407
0.073049075
0.016826102
0.061107841
0.4502786
0.384125546
0.013692473
0.658809982
0.016274729
0.018403753
0.381816358
0.509581681
0.001712259
0.077564172
0.171111689
0.008707521
0.135155087
0.838639518
0.045839837
0.005930637
0.098735672
0.067236839
0.000379298
0.013192472
0.004577611
0.002080704
0.03875105
0.045204955
0.006694241
0.029534075
0.001392531
0.000197738
0.017453519
0.03296751
0.039921167
0.012483902
0.000239447
0.053981569
0.001576685
0.00358937
0.046611047
0.035755592
0.034709941
0.003931311
0.007475961
0.023919478
0.013197548
0.017813548
0.060013597
0.00431506
0.12650109
0.008687253
0.022041561
0.333350829
0.563277293
0.000563867
Continued
172
Table A. continued
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
0.000487728
0.000892925
0.003313716
0.056985654
0.013513204
0.009665909
0.019066073
0.00805707
0.124099488
0.066122362
0.017801431
0.391462514
0.000170232
0.00699417
0.012718877
0.045200015
1.207396538
0.001272518
0.056898624
0.007164139
0.001988267
0.050288073
0.002426513
0.061594851
0.052140711
0.000614763
0.022395747
0.006148843
0.009718572
0.02935791
0.009351176
0.000383137
0.004912525
0.000134575
0.00131462
0.006684434
0.000181649
0.022465136
0.003030552
0.005559592
0.022325157
0.024411904
0.001519203
0.041369279
0.015239201
0.011948962
0.154941457
0.003986881
0.000847621
0.000717139
0.006106586
0.004203253
0.000981725
0.000113684
0.000189051
0.014630462
0.040064662
0.730775473
0.035887597
0.018039137
0.004898121
0.000144878
0.012102956
0.004389103
0.000299554
0.085626889
0.00140209
0.015160622
0.587951566
0.042409366
0.003746865
0.085209448
0.009595331
0.030016368
0.267121632
0.034661015
0.007420385
0.088438368
0.0292698
0.116234175
0.05703806
0.052388491
0.012054409
0.004115875
0.022986556
0.004825463
0.001861135
0.051107475
0.014320926
0.000588934
0.003697312
0.079675127
0.005090384
0.016784154
0.004501982
0.00651674
0.002000653
0.004656523
0.047597141
0.003313739
0.000215074
0.073181953
0.005309734
0.005293577
0.003848187
0.003851216
0.000840641
0.002829148
0.000127221
0.000206177
0.000814204
0.00463612
0.010013076
0.000191336
0.090847402
0.014818961
0.026773732
0.108872015
0.070692064
0.013813398
0.166098399
0.2501125
0.037361821
0.708893383
0.069868722
0.000436411
0.008457119
0.004516252
0.018311878
0.002421001
0.005829808
0.180715677
0.075433782
0.072442772
0.123255426
0.025057731
0.017332644
0.002819579
0.001627811
0.034054879
0.000381267
0.099323597
0.004267677
0.032253551
0.090967109
0.130103738
0.002623591
0.062444522
0.075400052
0.059447701
0.680456018
0.003002542
0.001417595
0.011767159
0.005412134
0.000417331
0.000191598
0.001003326
0.11866154
0.130469349
0.189771641
0.189045684
0.038034431
0.024663192
0.002017466
0.001621101
0.012060008
0.000162547
0.142903655
0.002479721
0.016411976
0.013453186
0.033690595
0.003136449
0.26750286
0.020576134
0.04485875
1.944302533
0.028430075
0.004711308
0.06967694
0.008775561
0.007354689
0.087411692
0.061849042
0.000730995
0.041010342
0.003639805
0.003254094
0.096108882
0.01065104
0.00264148
0.00848551
0.131260371
0.035731563
0.016709679
0.077621295
0.028799195
0.014426817
0.031611226
0.013397567
0.00444764
0.149210123
0.022258888
0.007051258
0.002829871
0.001316292
0.007145246
0.009143895
0.012372014
0.001011072
0.01517024
0.001656682
0.105044987
0.167076904
0.297681448
0.030800161
0.050644616
0.009860259
0.121120017
0.000178382
0.024784814
0.000222407
0.001842324
0.028454344
0.017971003
0.001354051
0.015974789
0.003877334
0.017297888
0.030944584
0.005659859
0.006084375
0.001354076
0.00099471
0.007845435
Continued
173
Table A. continued
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
0.730240237
0.035229494
0.013873081
0.003588706
0.000178388
0.014531401
0.001622196
0.008185156
0.000306528
0.024922776
0.000349397
0.026842002
0.091694249
0.000867772
0.013952114
0.280606546
0.008079104
0.007694309
0.003183887
0.184238831
0.011481883
2.143619907
0.022633839
0.007904474
0.003889537
1.072759531
0.01295367
0.005453166
0.007835085
0.005604581
0.004279635
0.002884108
0.009592082
0.004116745
0.007223695
0.005490347
0.000837554
0.027951889
0.09829335
0.015386204
0.007279442
0.096020957
0.006357958
0.009495105
0.010565389
0.053964285
0.163884943
0.019056714
0.00651134
0.095327067
0.008870165
0.007232177
0.001435985
0.001860413
0.007039214
0.088917557
0.001842655
0.0040842
0.135775137
0.004946263
0.008452097
0.307425144
0.001930621
0.007444286
0.450474273
0.003229018
0.001066271
0.001859374
0.012015069
0.403234706
0.011552058
0.100746426
0.046818307
0.001043082
0.003015705
0.000843659
0.110979616
0.004368401
0.187693842
0.037231504
0.0013447
0.000465562
0.003908465
0.000684608
0.003082095
0.002383671
0.010583852
0.004346291
7.44394
0.000902503
0.000375396
0.033383791
0.003831344
6.93464755
0.00202931
0.00074112
0.025013395
0.00959848
7.72409115
2.758076684
0.051967774
0.011117676
0.044388202
0.000413953
0.014765859
0.012288708
0.031697735
4.626782623
0.03981533
0.027093072
0.002457333
0.001183104
0.02834414
0.008955501
0.007292493
2.438901037
0.108028675
0.022761607
0.001112918
0.002655551
0.337895424
0.005966658
0.033477271
0.006115932
0.001161091
0.075437939
0.245991278
0.005767061
0.007362307
0.135547092
0.010324299
0.007174038
0.003199552
0.010129186
0.227052696
0.015855543
0.003321689
0.001489948
0.134201418
0.571337377
0.04089486
0.024036303
0.06619804
0.009656987
0.006465353
0.002825024
0.011396757
0.010969767
0.099021301
0.165409582
0.010342177
0.02807346
0.083407604
0.012053444
0.007622581
0.003548283
0.21696772
0.006569106
0.000487635
0.006274535
0.00252264
0.006249317
0.539294348
0.004808737
0.000316037
0.195760734
0.018951572
0.105219953
0.014918298
0.000473402
0.001324964
0.006645793
0.616346724
0.003000158
0.001594542
0.206098094
0.037260299
0.00095589
0.002913046
0.001304542
0.000517599
0.001059912
0.00205373
0.083420228
0.005350899
7.0264585
0.004308892
0.000666651
0.002071034
0.1405951
0.251553247
0.00586711
0.001197333
0.091600274
0.006962097
0.002400681
0.006236149
0.002102763
0.001412219
0.000865719
0.043011949
0.006846412
7.58747125
0.006441202
0.004851127
0.03351759
0.004123022
7.34983915
Pancreatic Tumor
OUP42 (T)
0.688322831
0.012142239
OUP105 (T)
0.011153874
0.248999322
0.003610824
OUP122 (T)
OUP123 (T)
OUP135 (T)
0.236854308
0.004198746
0.066422711
0.080029484
0.013842985
OUP139 (T)
0.402757796
0.019092422
Continued
174
Table A. continued
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
0.014714647
0.000195859
0.100287331
0.000404457
0.106961927
0.003271698
0.090000528
0.097010705
0.058387699
0.031256992
0.000388743
0.402919007
0.041234287
0.050717318
0.000161224
0.015558068
0.061154407
0.044129945
0.408619446
0.038267919
0.081200773
2.298142519
0.037793941
0.00600025
21.08385499
0.043107696
0.131323341
0.023038958
10.83605915
0.074282582
0.017757543
0.02275712
0.119314747
0.00247522
0.179349455
0.014041553
0.169153012
0.104675359
0.035200525
0.05121525
0.049790654
0.017323169
0.00015392
0.068949563
0.00304275
0.120782448
0.001138562
0.03702743
0.128698908
0.002127454
0.107542977
0.036693205
0.00211759
0.012855974
0.047822114
0.072867976
0.02495436
0.045896429
0.065482046
0.122116768
0.054911732
0.025263034
0.676080162
0.048105619
0.001176736
0.000174162
24.11291594
0.04812201
0.102545829
0.069965995
14.83697133
0.043745433
0.019216597
0.046647246
0.139287454
0.008746916
0.074022066
0.000499387
0.024515478
0.085862573
0.035320087
0.011324834
0.065614784
0.034388267
0.000627595
0.016879861
0.001820256
0.047733811
0.000922099
0.036984275
0.001407034
0.013058602
0.109011884
0.000597795
0.136596964
0.063844419
0.006107399
0.44454067
0.134174723
0.138929639
0.000537159
0.019885433
0.008106494
0.050257469
0.162899583
0.139097589
0.028738863
0.489542432
0.0524743
0.012676799
0.001397596
0.000904387
27.11143966
0.030614795
0.162772775
0.101711931
8.224633086
0.05960575
0.008900541
0.096765013
0.13460961
0.010342386
0.039110216
0.003487473
0.037383924
0.068731575
0.023997024
0.015128993
0.223803161
0.035498203
0.002387511
0.037614622
0.018973734
0.000841361
0.001136373
0.035167606
0.005301019
0.029222095
0.032762277
0.039732239
0.041980711
0.040229341
0.026049995
0.026616652
0.041261524
0.093360035
0.061869403
0.005222739
0.195482236
0.019596943
0.018691063
0.000376859
0.048940611
0.063442367
0.009316543
0.000194888
0.050836497
0.024094237
0.011105552
0.140554634
0.111340272
0.003824657
0.032965458
0.006679831
0.035976931
0.109966875
0.114243574
0.017970513
0.279761224
0.027302728
0.012386384
0.045821577
0.002790285
0.077217487
0.001784704
0.160077719
0.001834153
0.044855149
0.059622737
0.044438623
0.003893446
0.227010498
0.10498524
0.099447294
0.070620553
0.048207366
0.062363833
0.307499276
0.052586304
0.012490988
0.74350684
0.024943469
0.00093186
0.0009279
15.06278798
0.019061988
0.044849817
0.022495245
3.472530629
0.024737893
0.004484721
0.060787244
0.075082028
0.010647182
0.033399526
0.000507017
19.98249633
0.016213894
0.086546688
0.045016429
5.262918009
0.034130087
0.01129557
0.080537803
0.09326124
0.004371646
0.031124024
0.000134285
35.43957553
0.023713352
0.265839782
0.165166433
11.16399067
0.058610742
0.031360881
0.06452855
0.157295077
0.003794492
0.061580544
0.021832058
0.098531335
0.025358398
0.012044129
0.061077419
0.002220442
0.019133557
0.040527105
0.014498037
0.009745699
0.079761366
0.007513454
0.005849646
0.115240954
0.06127016
0.187569209
0.029697163
Continued
175
Table A. continued
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
0.029164967
0.043462523
0.000780442
0.02889291
0.001631532
0.036105838
0.000859591
0.020694281
0.001109632
0.053524414
0.000522144
0.006665484
0.000863784
0.051227091
0.003585117
0.040894929
3.556540936
0.015863454
0.40525005
1.402372168
0.003569446
0.011679865
0.006086105
0.001322151
0.024299677
0.008648759
0.148826869
1.053010571
0.002565888
0.096843727
0.263521197
0.022788597
0.082473156
0.460798817
0.013864274
0.000270455
0.145761581
0.948884845
0.005569042
0.000358526
0.000951352
0.138866477
0.839665298
0.001121142
0.02832441
0.008257219
0.071872801
2.039094616
0.012654477
0.270088893
0.458825894
0.000479077
0.106064827
0.659709013
0.00480088
0.504079528
1.99626157
0.003581982
0.235635812
1.435887499
0.001794761
0.006805377
0.003697936
0.06847102
0.011091958
0.316341628
1.688527668
0.017117607
0.349223323
0.508039586
0.001160504
0.072316458
0.576691362
0.002384874
0.002705627
0.193303856
1.24187229
0.006841106
0.004069764
0.137858466
0.687723128
0.005064144
0.775452205
0.254498184
0.079265109
0.000213193
0.01462178
0.017024365
0.002902601
0.071470405
0.002673943
0.002362342
0.047638053
0.014094638
0.05486412
0.782894476
0.002300581
0.000596661
0.094014117
0.538242314
0.00106304
0.259144238
0.487857164
0.065503428
0.003483577
0.022534749
0.024179869
0.00128909
0.034558507
0.017492505
0.010749883
0.071040632
0.009156892
0.007979097
0.017599134
0.001143579
0.000116578
0.004433939
0.002231204
0.102931918
0.202496702
0.000804029
0.284959999
1.517319411
0.02570827
0.003364488
0.133509316
0.112037905
0.021757443
0.006556369
0.017410794
0.019756995
0.130854035
0.024439309
0.01601553
0.05423084
0.027448894
0.00024647
0.025558223
0.00698847
0.04589696
0.044163886
0.000535104
0.17001046
1.593874369
0.011110383
0.003775118
0.015615845
0.031212061
0.002630986
0.012296222
0.013269887
0.010868235
0.035424527
0.011381902
0.064516654
0.025889034
0.000974664
0.00020945
0.006822657
0.003181201
0.020230247
0.026303667
0.013808584
0.023098408
0.086199554
0.006154301
0.059933931
0.067875081
0.012339677
0.015194425
0.017577381
0.014210119
0.059266942
0.816042061
0.007352777
0.146795985
0.167851626
0.000243918
0.065702379
0.331092772
0.003385044
0.084574434
0.722537524
0.000669585
0.030027245
0.051385724
0.079755174
2.385665741
0.028262461
0.006075157
0.098493131
0.117510243
0.001409979
0.00041139
0.036008752
0.004935281
0.096600899
0.039661139
0.00662366
0.104301508
2.129961597
0.018004314
0.326844181
1.040547929
0.003442366
0.126623178
1.137816904
0.007154315
0.317673559
2.270244089
0.001514441
0.000692076
0.088934495
0.303207095
0.011317369
0.408178591
1.753283652
0.104063311
0.002646146
0.030059158
0.038951574
0.017848794
0.005514002
0.009496232
0.006026021
0.034224444
0.011376763
0.102299743
0.034199699
0.003740879
0.000448854
0.006249835
0.021613266
0.049406406
0.06803052
0.019160877
0.037645466
0.131543642
0.01762261
0.013439189
0.001004018
Continued
176
Table A. continued
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
0.006141817
0.021075005
0.001371395
0.001875832
0.000490003
0.034533019
0.01285908
0.104112325
0.005297955
0.021103546
0.007463242
0.03810684
0.34355602
0.295406867
0.003320301
1.8092199
0.034626437
0.00650917
0.111836984
0.253484734
0.000140749
0.867642254
0.000216433
0.000177472
0.002118387
0.055854612
0.014443005
0.029983398
0.001235936
0.032029681
0.013054276
0.040433397
0.144719298
0.174006635
0.003466467
0.36510978
0.013416868
0.018151244
0.26769159
0.571298133
0.40671788
0.087233671
0.146327306
0.012228407
0.006395987
0.057463386
0.086374114
0.036489111
0.007762574
0.061538446
0.010169851
0.076242029
0.061952329
0.229481828
0.005242987
0.213300806
0.003729239
0.069584212
0.502010108
0.871751786
0.0011076
0.014023822
0.009903625
0.034314649
0.017665182
0.003204711
0.006522508
0.039037499
0.008351965
0.018287129
0.029434816
0.113669682
0.00469798
0.404927609
0.008781873
0.024543783
0.402068277
0.222153522
0.000503286
0.000465485
0.002903713
0.008355729
0.018214673
0.069421696
0.022706583
0.010315903
0.02640672
0.033556776
0.011788753
0.066867891
0.122260766
0.005529986
0.154220282
0.006127556
0.054876277
0.558487509
0.518808283
0.000357186
0.000601966
0.009788881
0.035316954
0.098385105
0.030308573
0.515488525
0.013630655
0.002015532
0.005096535
0.019746158
0.002085518
0.256608403
0.026677769
0.082465833
0.258893889
0.247770154
0.01326493
0.410063949
0.028148538
0.084343975
3.189947807
0.039301994
0.000977821
0.003426759
0.000760393
0.00282715
0.001424343
0.068948808
0.009978592
0.008198623
0.168122756
0.037168864
0.010915189
0.126582899
0.014269587
0.018338379
0.454580725
0.032514745
0.000249069
0.002774125
0.00210141
0.000320314
0.000170272
0.198336172
0.088092024
0.027315494
0.021473041
0.006888821
0.000983384
0.337325274
0.324387767
0.009332164
0.000283769
0.000670541
0.002823915
0.379640397
0.065680887
0.000785985
0.001038721
0.022215482
0.000197003
0.025015884
0.0025842
0.007672252
2.979555807
0.042846161
0.005221231
0.032327814
0.027329877
0.283689718
0.020598824
0.003775958
0.004495986
0.001038263
0.007976526
0.064105359
0.024666225
0.000142585
0.003912362
0.004463101
0.00019211
0.06126635
0.00253663
0.020286408
0.252353713
0.005560321
0.002278155
0.000897184
0.175923537
0.013863184
0.036655079
0.056456751
0.270062254
0.070886191
0.377579782
0.022799112
0.10655834
1.487552844
0.011597936
0.000318477
0.000934692
0.024473036
0.093822738
0.000327634
0.247647235
0.171249588
0.99943796
0.001161194
0.005259396
0.006930076
0.039426458
0.025285111
0.056800617
0.00304762
0.178600549
0.015329473
0.047374414
0.171104003
0.292324554
0.004434762
0.461689049
0.011673087
0.01811862
0.43384359
0.530816794
0.000993338
0.002324476
0.001542617
0.033789491
0.000459821
0.096333068
0.011072873
0.047218546
0.389062927
0.110716252
0.015912777
0.042773347
0.068797093
0.043853762
0.643583578
0.050700891
0.000835879
0.01480281
0.000404397
0.00438929
0.001136457
0.090546044
0.138424807
Continued
177
Table A. continued
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
1.734100809
0.172383043
0.005838752
0.003182246
0.006268814
0.251638351
0.047836287
0.020846106
0.012959871
0.006914431
0.299386862
0.089505294
0.091150717
0.032184901
0.010009143
0.243900466
0.057114957
0.048397855
0.013474303
0.002249678
0.021787023
0.027733748
0.097374878
0.018900771
0.013410756
0.339788968
0.186163858
0.037349362
0.019979114
0.005669772
0.091817074
0.004857408
0.004110083
0.228098722
0.140955558
0.00136571
0.011965282
0.00334642
0.000334262
0.064131403
0.007376687
0.003009381
0.000325725
0.035684682
0.009317029
0.000115645
0.091094245
0.037316984
0.00363094
0.035757625
0.000189265
0.002609252
0.047687046
0.010333911
0.008773616
0.003751419
0.013744921
0.009537877
0.000495082
0.074026104
0.019984897
0.001440352
0.010143008
0.027043511
0.004129905
0.016453203
0.007169517
2.546448816
0.090303093
0.020212551
0.016226051
0.000328991
0.010072424
0.057564342
0.007779463
0.016049253
0.012689956
0.005868884
0.086007806
0.011469926
0.000802095
0.031984504
0.007836955
0.029695753
0.084813188
0.006302511
0.010286218
0.008372136
0.00221209
0.004543907
2.96830002
0.026635375
0.021689813
0.029178245
0.00037133
0.026340589
0.008400692
0.006723189
0.068160603
0.002892494
0.000665098
0.061330262
0.022403958
0.001881075
0.047172011
0.003101203
3.098310579
0.038619459
0.007975534
0.004211059
0.000109879
0.000624876
0.071945999
0.242215529
0.044457443
0.006488071
0.024943838
0.006717009
0.003931997
0.001574375
0.008518767
0.131031063
0.001305691
0.007463625
0.004451165
0.043825253
0.139297012
0.024027489
0.01858919
0.044033222
0.007929638
0.003504681
0.004425354
0.01407022
0.203429346
0.008552629
0.041428613
0.003553838
0.001746064
0.198320857
0.025880464
0.001180088
0.031122577
0.001629796
0.035914726
0.054432791
0.002505876
0.010203506
0.002204114
0.000313573
0.012966023
3.974356586
0.104044587
0.03895901
0.030837494
0.000429908
0.056335898
0.006762174
0.022774305
0.00070767
0.016655752
0.00881761
0.073350333
0.143854157
0.020423088
0.037761119
0.129453764
0.017574504
0.006263242
0.004797315
0.019133277
0.301997041
0.047106887
0.000677376
0.001036425
0.010471546
0.003713663
0.003388719
0.00964173
0.005261715
0.004931668
0.007176307
0.036190213
0.005827179
0.010700622
0.00031053
0.002101205
0.004122146
1.856676438
0.025876045
0.016909366
0.008372362
0.000197371
0.005480001
0.001432638
0.00422324
0.000354743
0.000356383
0.026386283
0.097001403
0.008155005
0.014685512
0.116897414
0.012526619
0.003507165
0.00377174
0.001249833
0.1087202
0.012208002
0.00227729
0.002253046
0.036534706
0.074171894
0.008306687
0.037042093
0.171418761
0.010567031
0.003840721
0.000378412
0.006322294
0.001164899
0.00444107
0.005542282
0.082204969
0.064540851
0.009572082
0.074209794
0.010483167
0.005688616
0.003688501
0.000464874
0.009680685
5.747712576
0.067894636
0.020786071
0.019742389
0.000707421
0.013101496
0.008882047
0.008870403
0.002048544
0.002279318
0.100194444
0.338029935
0.022786915
0.022758802
0.151873169
0.013935011
0.011859758
0.019324011
0.212060294
0.013701374
0.001460952
0.005042451
Continued
178
Table A. continued
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
0.621788039
0.017909244
0.000355776
0.361800867
0.114597682
0.961455652
0.013897982
0.000167964
0.314748895
0.065735236
0.000503092
0.000392014
0.013105032
0.000945061
0.000292133
0.001244667
0.001415262
0.068299684
0.000210693
0.028173064
0.003850515
6.7847897
6.89571015
0.63752765
0.011774445
0.356709445
0.09543669
0.216697951
0.000973283
0.000539144
0.126581188
0.011207983
0.160983577
0.00211521
0.000590746
0.080886285
0.008679135
0.008290006
0.001596799
0.005148446
0.017058644
0.007030621
0.040104719
0.006943904
7.61849425
0.000300506
0.000483469
0.000381008
0.000608045
0.000708415
0.003977816
0.002634194
7.23457885
0.000678756
0.001526976
0.00178303
0.009283818
0.002881546
0.031530698
0.000219709
7.40222035
0.576807736
0.011833018
0.000443352
0.229311151
0.065920702
0.005382904
0.002098368
0.000506535
0.00757575
0.002672606
0.060103296
0.012170029
7.14890985
Pancreatic Tumor
OUP29(T)
0.005190609
0.038127954
0.001969118
0.009728148
0.045355692
0.001535123
0.070529075
0.02539987
0.042260464
0.0002544
0.109124044
0.049739573
0.000891226
0.000744681
0.110610321
0.072559404
0.003474616
0.003757459
0.010921296
0.099645117
0.140006957
0.10268746
0.019504184
0.348734601
0.039289426
0.001438642
OUP37(T)
0.019623511
0.407206083
0.04808016
0.091165168
0.002783066
0.069931173
0.001146975
0.100014114
0.007991821
0.010809743
0.085996188
0.005505491
0.074538341
0.017149259
0.000856129
0.00026217
0.12187837
0.131555778
0.000214548
0.040425839
0.074715274
0.132851002
0.208287046
0.219125383
0.204083863
0.337874182
0.139518807
0.028035916
OUP49(T)
0.003227546
0.049689959
0.004766276
0.014240614
0.001314505
0.042285612
0.00211911
0.054580207
0.001829775
0.007668925
0.033767617
0.000494027
0.090779048
0.028647969
0.000781181
0.275813823
0.091140252
0.06256832
0.01506602
0.026593116
0.061040546
0.085861975
0.060563994
0.028559404
0.223818651
0.018837805
0.000243213
0.000931787
OUP101(T)
0.004840743
0.116258872
0.008299529
0.012433176
0.001053485
0.038554477
OUP119(T)
0.007383645
0.208928668
0.013157488
0.025092967
0.00140158
0.051585248
0.051462656
0.0040218
0.01621097
0.025821296
0.000274864
0.029781665
0.007729912
0.000719643
0.052827125
0.032755244
0.02067369
0.000193969
0.001195833
0.004688968
0.011962529
0.037565216
0.160868272
0.01072776
0.347720064
0.048122694
0.099020763
0.003995694
0.023601647
0.044466291
0.000178149
0.117847525
0.020364961
0.002880158
0.673031703
0.081172163
0.050663546
0.007432694
0.03801286
0.041151943
0.047807271
0.097333778
0.079899865
0.006482243
0.428829741
0.031246564
0206C077B(T)
0.006690052
0.377440612
0.011266257
0.031333084
0.002431742
0.069894298
0.000875175
0.093238724
0.00470112
0.028242069
0.093194658
0.000280116
0.097306978
0.01316299
0.002650389
0.886936712
0.105712367
0.048182033
0.013890026
0.025763967
0.026761793
0.130061157
0.082778968
0.008380698
0.737244505
0.031113652
0.003023003
0.000956506
Continued
179
Table A. continued
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
0.001198682
34.25400846
0.005918232
0.105803659
0.016958042
7.496719482
0.022896945
0.003935454
0.086423168
0.078799862
0.001740466
0.067934556
0.003833154
18.76520335
0.068903521
0.246958584
0.34063711
7.749668072
0.204638999
0.009481296
0.112039396
0.257413125
0.007598638
0.037752317
0.000885174
20.82220098
0.016998034
0.085955107
0.038576864
6.374734114
0.026981646
0.017063
0.107833524
0.090297394
0.004308622
0.023073756
0.048786149
0.062626342
0.037793696
0.011491177
0.100288766
0.037866737
0.086298185
0.096236969
0.077349888
0.023820591
0.262323147
0.026628612
0.000223873
0.056446581
0.002073332
0.034542125
0.035588222
0.016060083
0.026423606
0.065718263
0.015114328
0.00097476
0.017826245
0.003953876
0.00040551
2.393053614
0.078240382
0.043038414
0.0730886
3.148811117
0.019044988
0.012921248
0.103600633
0.05640388
0.001257993
0.019905276
0.000339086
0.01684911
0.028703415
0.011452765
0.026175941
0.049346454
0.024350137
0.000284058
0.008154249
0.000369023
0.006928516
0.003511438
0.098722245
0.000638801
0.430595611
0.973202897
0.013804773
0.310688559
0.428107204
0.001342611
0.114828767
0.57061557
0.00737148
0.001006782
0.441471901
1.151386354
0.032485957
0.000338755
0.002765688
0.111372775
0.147056914
0.009280415
0.063859343
0.007802262
0.0014085
0.018152873
0.024420697
0.242925639
1.160151062
0.0140718
0.476092455
0.265544245
0.000272736
0.076910995
0.510652463
0.004763688
0.000423229
0.006566841
0.000391646
0.094082142
0.425766463
0.004592343
0.175282725
0.101050245
0.000280868
0.139956628
0.085221498
0.00879835
0.168598391
1.010081126
0.007686839
0.064563324
0.141182352
0.000722788
0.179187595
0.025757001
0.000700295
0.029213397
0.002895011
0.161364812
0.947134304
0.019101582
0.40134892
0.211274041
0.002961118
0.046652507
0.405694717
0.005538728
0.1791513
0.941212848
0.001104128
0.000601161
0.001791065
0.061475907
0.17824888
0.130255926
0.004116999
14.5300259
0.0202839
0.070819102
0.023125748
7.033724702
0.036817931
0.003090214
0.058461103
0.069804477
0.007344902
0.017180899
12.03163724
0.045954631
0.130900687
0.07360801
8.963695056
0.042508807
0.008232812
0.057845321
0.175987037
0.086672376
0.008045514
0.078220462
0.035321303
0.005994682
0.046529964
0.015330912
0.023768474
0.087719906
0.041628246
0.012371962
0.078961629
0.045920147
0.024502306
0.000376257
0.048447132
0.000538527
0.006364699
0.024185903
0.232323053
0.000772301
0.012573311
0.014332875
0.058448219
1.244553604
0.004946065
0.281551135
0.538468318
0.000486862
0.047470593
0.404894477
0.00414743
0.001621932
0.129034152
0.986303713
0.006234832
0.017271606
0.000392524
0.04515536
1.44326485
0.005596537
0.293147583
0.807868884
0.001501755
0.089101194
0.626518306
0.009998342
0.000248733
0.087929317
0.981638385
0.010088224
0.007078759
0.019929001
0.173835449
0.013408412
0.03702444
0.06914757
0.306352041
0.020055933
0.219193568
0.000267038
0.063029109
0.835943632
0.005538385
0.242719945
Continued
180
Table A. continued
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
1.484400528
0.031267722
0.004928695
0.067096807
0.061839541
0.001500851
0.011074312
0.029171855
0.004969662
0.067450639
0.011163857
0.025364498
0.017629776
0.006326062
0.039695586
0.024379797
0.054568811
0.041143103
0.000340943
0.207925113
0.016217519
0.008807211
0.005638792
0.041821016
0.03441369
0.010201523
0.015194841
0.007878452
0.00984527
0.081727642
0.195881414
0.007708412
0.715197659
0.045100052
0.027311851
0.494075499
0.562481643
0.00107626
0.000465782
0.007527693
1.85785965
0.079543482
0.008788731
0.242136256
0.22541103
0.004873544
0.011920595
0.02653447
0.045104792
0.118682203
0.011145754
0.102976784
0.018391864
0.001240787
0.000256382
0.085839324
2.045772429
0.034319948
0.008531057
0.065352406
0.034280184
0.000751554
0.011117939
0.009347392
0.010187181
0.061402137
0.018611068
0.031368235
0.035402748
0.00258976
0.000967204
0.010132793
1.492194111
0.013608225
0.003250506
0.036145033
0.018910282
0.000679396
0.022667243
0.011819974
0.005038628
0.024852016
0.011315493
0.000784901
0.009165989
0.003558575
0.000590038
0.009215092
1.069952959
0.049869753
0.002162373
0.011779906
0.017735593
0.000597797
0.016798135
0.014615762
0.007459144
0.033244255
0.016060915
0.00851511
0.627872647
0.004106527
0.000213566
0.013239146
1.01554669
0.106473482
0.001751919
0.025150826
0.023566373
0.002260342
0.029370967
0.013232162
0.019875598
0.089753597
0.02613233
0.00603735
0.029188706
0.00163034
0.016797706
0.037573687
0.039377546
0.000194942
0.304479292
0.005721858
0.001820846
0.004260692
0.098504915
0.116085446
0.039254722
0.01040586
0.058774351
0.010008364
0.016898658
0.058689553
0.041271624
0.011374221
0.183032852
0.009707507
0.068477793
0.350749273
0.870979712
0.003872087
0.000885221
0.000163823
0.00127826
0.024213871
0.01855991
0.010101772
0.009521425
0.02549462
0.009816526
0.006775611
0.008035083
0.000391057
0.105623717
0.004855319
0.015988793
0.046078701
0.000169911
0.297665385
0.001159237
0.027419265
0.031909653
0.017671805
0.009232815
0.000601256
0.013387099
0.009809387
0.026914692
0.015283425
0.010416661
0.002292759
0.041563536
0.004367593
0.016901765
0.541015457
0.395942467
0.0155992
0.002453605
0.045084204
0.014397927
0.024062951
0.000689154
0.021457047
0.00336995
0.015022918
0.041427552
0.090044338
0.003490386
0.105238308
0.010918904
0.019659976
0.313552813
0.573038458
0.003757123
0.035722183
0.024500858
0.000307827
0.241356832
0.001476033
0.012452309
0.002168032
0.074340081
0.017973523
0.026756414
0.005617956
0.011286735
0.026994112
0.062383684
0.159247769
0.133507164
0.003929164
0.07361676
0.001686843
0.021546795
0.314587242
0.442642557
0.000479486
0.000206894
0.000267771
0.00020454
0.000501319
0.00251124
0.000291665
0.008512298
0.002367452
0.008577691
0.12503238
0.002687878
0.008622107
0.010296424
0.02735182
0.045990467
0.038282097
0.008662335
0.017610866
0.025512767
0.011973014
0.048194946
0.133374214
0.005134209
0.495278592
0.013534751
0.022078878
0.705486152
0.403696552
0.000669155
0.000274675
0.002418818
0.002387051
0.00057251
0.006827961
Continued
181
Table A. continued
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
0.001496059
0.137360387
0.008171037
0.026680581
0.453012078
0.210408212
0.007105062
0.30953746
0.032431261
0.057802469
1.311363749
0.027078853
0.001014449
0.0091206
0.078338967
0.005701337
0.147353008
0.152433925
0.374247328
0.09421019
0.074213575
0.006499662
0.003528071
0.031779361
0.002538328
0.000501464
0.161433726
0.017272628
0.001000929
0.03325865
0.004824083
0.019769099
0.048498046
0.007969298
0.012195907
0.001334648
0.017652269
1.333289823
0.027577738
0.030104812
0.026115161
0.002737784
0.273272752
0.013876856
0.062430508
0.040426259
0.266344246
0.083157547
1.222164532
0.077353454
0.160770991
3.522129658
0.0173219
0.006108869
0.001404067
0.07692309
0.802624733
0.004511863
0.00132666
0.657721629
0.374236588
0.072225521
0.048527001
0.139634469
0.009180255
0.019598316
0.020618619
0.002100193
0.001492289
0.09394564
0.032556717
0.005271949
0.050340785
0.039016104
0.069138112
0.033301259
0.001694076
0.015725482
0.00448576
0.00680626
0.014639149
2.770880551
0.048903839
0.038942964
0.037412314
0.000805373
0.000654082
0.128861363
0.020576325
0.005617472
0.366569376
0.125610561
0.010905828
0.768696588
0.027146108
0.07737518
1.821912495
0.047295826
0.001194732
0.026637014
0.001323626
0.000603529
0.226861574
0.079315997
0.206226076
0.045657152
0.119461378
0.015106421
0.002926614
0.044638804
0.009224038
0.001805237
0.032224743
0.027655748
0.005754667
0.043138809
0.00118709
0.001770341
0.027041391
0.001175164
0.019016238
0.003846231
0.000250321
0.005125735
1.899444646
0.04436249
0.033462039
0.005776934
0.00047485
0.000256153
0.033313084
0.003345235
0.002109657
0.046727324
0.004856889
0.825172827
0.028835457
0.014085135
0.677648115
0.021880966
0.001233378
0.004619786
0.001752852
0.000179183
0.022569358
0.106724345
0.631667048
0.011913096
0.042010571
0.084536912
0.000689051
0.019362314
0.000284806
0.061247462
0.002927246
0.072196308
0.07247998
0.03972293
0.004341597
0.159207408
0.069276837
0.027996298
0.493509195
0.003196334
0.002618399
0.000549224
0.074372371
0.001238501
0.016817838
0.022554679
0.029588894
0.03062434
0.08751712
0.105692328
0.043092768
0.404491578
0.02120144
0.001483928
0.004513416
0.00834341
0.017811046
0.000892431
0.001901606
0.000508886
0.024982741
0.107526754
0.841938389
0.041556373
0.03406277
0.014011285
0.004980839
0.000333249
0.084295818
0.086729576
0.266144908
0.040649146
0.038571949
0.017175886
0.005283735
0.001532295
0.032882407
0.01781203
0.00023975
0.036399457
0.002042393
0.009962446
0.005245679
0.004820923
0.013433371
0.000438636
0.064471411
0.013575432
0.000325151
0.072310796
0.028232591
0.000238844
0.048762668
0.006701775
0.001115395
0.025413271
0.011628057
0.007256992
0.007442922
0.00492222
0.897989648
0.041554606
0.016988888
0.00546184
0.000817109
0.004100879
2.634677484
0.047698753
0.014956183
0.02103173
0.000254828
0.166662631
0.001622782
0.095200738
0.055230771
0.000614094
0.022543019
0.003253258
0.00506267
0.043455047
0.008726075
0.009015037
0.001301518
0.000227788
0.007790775
1.73986886
0.100927769
0.017618227
0.012368189
0.000355397
Continued
182
Table A. continued
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
0.046246898
0.017082541
0.006654334
0.000852917
0.001122829
0.279239883
0.086971343
0.013799377
0.022259514
2.088388934
0.013321274
0.004086766
0.006224171
0.125828481
0.016477097
0.002173051
0.004505106
0.179836145
0.00154463
0.001602331
0.081108809
0.041974368
0.002432487
0.001153561
0.003656171
0.004173199
0.010986506
7.42572085
0.062345426
0.011241135
0.052383673
0.000178061
0.010751219
0.010599592
0.349018283
0.131609316
0.026200761
0.138467233
0.800109311
0.023841148
0.009473144
0.102021457
0.044126542
0.000362248
0.003063609
0.00491866
0.002259283
0.156697603
0.014921884
0.00138221
0.088753635
0.013828893
0.000226291
0.00942344
0.00635137
0.000596919
0.016855457
0.007779489
0.008345999
0.006625752
7.29846145
0.03438071
0.005832929
0.006772772
0.309404314
0.0008414
0.019607926
0.01134404
0.007572597
0.008511628
0.066085019
0.003904443
0.005850364
0.001372181
0.002687488
0.31886655
0.101426755
0.006408532
0.013055424
1.985488726
0.019682894
0.006236316
0.000908156
0.000524079
0.109038662
0.021318558
0.003720402
0.004858061
0.227095767
0.082402317
0.000702628
0.017578676
0.580464692
0.027925012
0.005012577
0.008908203
0.011952492
0.136238817
0.011342871
0.003025312
0.238741102
0.184531226
0.001868692
0.018288029
0.522643956
0.009957625
0.018772816
0.002494841
0.011321594
0.113931957
0.002805517
0.00462012
0.00291603
0.302493819
0.260496441
0.022003526
0.011513163
0.602309354
0.017845586
0.009228476
0.003868248
0.006458143
0.231580562
0.007352429
0.004324189
0.00025829
0.012703661
0.172329628
0.001945539
0.001314221
0.078844568
0.015633268
0.000416741
0.002685371
0.000754455
0.000388749
0.003746491
0.001162248
0.003322422
0.004388368
0.003187707
0.00184673
0.044787477
0.415869481
0.020150329
0.001657973
0.551318672
0.011231846
0.004003088
0.131021536
0.039316205
0.016360709
0.859904333
0.018646777
0.000231394
0.409344543
0.126424946
0.006875509
0.00131812
0.000269839
0.000314766
0.00174003
0.015458179
0.002141019
0.000176869
0.005676615
0.003106914
7.7145896
7.46156665
0.000291427
0.001340675
0.026692206
0.00399033
7.5298803
0.001376291
0.001446238
0.07555076
0.006436585
7.5777325
3030396A1(T)
0.003588572
0.101835662
0.007187211
0.032833236
0.003410567
0.051607848
0.003173779
0.073737191
1050005A2(T)
0.007406153
0.202099418
0.005254625
0.029841184
0.001464899
0.039624666
0.001016213
0.058947587
0.194429323
0.06770425
Pancreatic Tumor
0206C138B(T)
0.005133434
0.067724306
0.0027362
0.014200077
0.001296226
0.044361379
0.002330971
0.05739057
3030401A1(T)
0.003980403
0.036461071
0.004138353
0.036120064
0.000571947
0.044300238
0.090066803
3030800A3(T)
0.067871915
0.002825296
0.00652169
0.001048809
0.010683169
0.021603517
Continued
183
Table A. continued
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
0.003214436
0.022313815
0.010710417
0.000185864
0.107157298
0.013396594
0.003656055
0.53349495
0.083248554
0.050843654
0.007838058
0.002036612
0.012348953
0.061886649
0.074056928
0.077356188
0.635436347
0.16067212
0.000269706
0.000514895
0.001149537
7.059918452
0.051382398
0.098726933
0.081223979
5.526066867
0.021747125
0.010227104
0.07118892
0.120185605
0.002501705
0.048300821
0.016969131
0.092656373
0.027047865
0.014283026
0.074932506
0.043981604
0.000198075
0.02823966
0.001168115
0.006951167
0.002704837
0.026479979
0.013920171
0.001153697
0.132953095
0.016516769
0.003294781
0.151614619
0.171092244
0.105340511
0.000208292
0.036153516
0.018853312
0.07410955
0.079434265
0.14150209
0.129454881
0.20012763
0.045579022
0.002018759
0.000546007
5.288735295
0.067656543
0.066388022
0.105518493
4.913434315
0.066744037
0.011032276
0.106845109
0.102356439
0.006366796
0.061244146
0.000250855
0.030801091
0.161932926
0.030434517
0.02732231
0.28572284
0.053500818
0.008009262
0.040476
0.004087468
0.050434902
0.067297922
0.051055264
0.000988303
0.004864514
0.84002171
0.011364654
0.011572646
0.110791781
0.004297732
0.001307652
0.373098191
0.024435158
0.007623321
0.019576376
0.018484919
0.016048419
0.059219619
0.005759932
0.142691967
0.002251513
0.00480031
0.040761354
0.028097971
0.209230093
0.045478351
0.010224537
0.905631955
0.000610185
0.002199803
6.270571861
0.014312386
0.083639487
0.008041409
2.972515884
0.007090905
0.000944987
0.042593778
0.041785393
0.003453827
0.017380791
27.81134803
0.004882434
0.374299174
0.014433758
26.88841425
0.029942682
0.001062035
0.035444812
0.500900261
0.002257449
0.072455714
25.16211119
0.033923999
0.190266183
0.051110006
9.075235426
0.014042081
0.015271293
0.077575428
0.140819309
0.00029716
0.039529063
0.003032602
0.018912587
0.010453755
0.001281762
0.047815553
0.039842259
0.005915813
0.026160089
0.03415543
0.000217769
0.030673153
0.010311222
0.068134938
0.048824347
0.026278341
0.017411539
0.093015004
0.000825941
0.004565357
0.000344679
0.00186516
0.021909848
0.004593812
0.002061849
0.021374309
0.061433635
0.006882548
0.046387071
0.014080512
0.000379573
0.182495471
0.100813522
0.051673112
0.005687138
0.030721673
0.031309354
0.03522079
0.19073904
0.077775931
0.015322304
0.835042522
0.030842052
0.000836654
0.000283057
0.084416163
0.029994755
0.000211416
0.061463344
0.000509186
0.004865847
Continued
184
Table A. continued
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
0.00388917
0.012538313
0.044343087
0.665067427
0.00478524
0.281366021
0.11912974
0.000949648
0.051854447
0.264510064
0.012632483
0.072825223
0.449040669
0.003615312
0.003027487
0.00736493
0.042875566
0.146649344
0.000902296
0.066831266
1.742168323
0.034823814
0.003209529
0.023414232
0.030384798
0.000470602
0.023327112
0.018503045
0.004548681
0.039301536
0.049037934
0.007500401
0.025374637
0.001529667
0.020397714
0.009456996
0.741084661
0.813998065
0.002577298
0.300048219
0.048838553
0.002790775
0.074882389
0.578820037
0.015088684
0.000406344
0.147488132
0.793146498
0.022703519
0.003054499
0.001271361
0.013738788
0.484337972
0.003095662
0.151934828
0.138879881
0.000239699
0.011944485
0.101264508
0.005728648
0.013815947
0.021052852
0.314224161
0.000660336
0.378509986
1.564766912
0.000436892
0.005461748
0.002037609
0.04503338
0.976102846
0.007069699
0.188814317
0.49795372
0.002136586
0.12253014
0.555660876
0.002636037
0.000325471
0.180477022
1.264302396
0.002225968
0.007949146
0.015719373
0.166565962
0.005037345
0.120851582
0.486989567
0.013831283
0.460292477
0.359269724
0.060452917
0.001279039
0.006121459
0.003852122
0.00369559
0.09817461
0.009463387
0.002140901
0.029215915
0.001443465
0.00181632
0.051625146
0.000208251
0.004510536
0.079071772
0.243003175
0.012544803
0.217992978
0.732704595
0.048565347
0.001034149
0.017715163
0.027757839
0.00052755
0.049866874
0.010844644
0.006807645
0.036916871
0.012758094
0.082854655
0.016697548
0.000691625
0.000465882
0.003734112
0.010632825
1.85252212
0.00515806
0.216656852
0.98025892
0.071704341
0.591232003
0.001913748
0.005898286
0.052009492
0.016371477
0.005595133
0.071358335
1.776435453
0.032421312
0.010520126
0.216043356
0.201631545
0.013946581
0.008476041
0.029893254
0.047295698
0.12592763
0.019368188
0.068118726
0.000760031
0.004043845
0.001700043
0.04686726
0.006446387
0.014210548
0.014404601
0.002644701
0.039088208
0.033038174
0.00112953
0.000691913
0.012179794
0.00473075
0.00042035
0.010536469
0.039513735
0.09587904
0.007052113
0.001782
0.004575077
0.026225081
0.007790487
0.002419915
0.016115894
0.052912268
0.000205069
0.229164515
0.002577803
0.001173523
0.011373709
0.002084363
0.001636001
0.003319025
0.113775239
0.721081143
0.017142704
0.003821411
0.002834036
0.001161742
0.000332304
0.06810448
0.004880026
0.006441822
0.015673058
0.033544267
0.000256484
0.001326426
0.000384512
Continued
185
Table A. continued
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
0.017268404
0.021688544
0.026248677
0.000390508
0.012879108
0.000402803
0.014063958
0.01728457
0.072811292
0.008649896
0.191805636
0.006405074
0.01502821
0.387898478
0.434041537
0.001018437
0.00051732
0.099431706
0.030066052
0.06441595
0.005797014
0.009310115
0.018667704
0.015213256
0.024495003
0.038602812
0.00858242
0.340861431
0.015948911
0.088288159
0.366531798
0.534320683
0.001032328
0.003991716
0.00392765
0.000632672
0.000564285
0.113982917
0.008944596
0.012494622
0.128627589
0.060867293
0.038616066
0.187336318
0.006763288
0.037678936
0.593061868
0.013768274
0.010609037
0.002466196
0.006792337
0.003288802
0.268037037
0.031997916
0.058818417
0.870294009
0.581706677
0.029845263
0.676234614
0.027034458
0.131216156
9.898159357
0.068792723
0.004393416
0.006929214
0.000594056
0.038646572
0.00161093
0.005270811
0.001947902
0.402440183
0.242531286
0.043223637
0.002607694
0.133342656
0.024192694
0.007802998
0.000295565
0.064894927
0.113925533
0.60471699
0.006946979
0.062290243
0.007927032
0.053821784
0.005512634
0.001459487
0.016791047
0.002143134
0.019896349
0.000249464
0.019151551
0.022991741
0.014243145
0.000630222
0.006684394
0.27972954
0.046497083
0.006305899
0.003403049
0.034768985
0.025789306
0.020269148
0.061518591
0.125347947
0.190192501
0.002383363
0.621496542
0.008188999
0.007154875
0.176576073
0.020914854
0.000334174
0.008818246
0.003126241
0.014028718
0.000176922
0.000131644
0.001903334
0.001957129
0.015097425
0.004074153
0.004522012
0.012715306
0.002985747
0.003660028
0.013107665
0.023794889
0.014612973
0.098839363
0.01161234
0.007098808
0.188282167
0.021805476
0.000317785
0.005886626
0.001475803
0.008295305
0.15236382
0.035155574
0.001482941
0.0007031
0.008689894
0.00022595
0.01932195
0.035062982
1.613536283
0.00318656
0.030005744
0.003311114
0.035077374
0.027743278
0.028912478
0.00038253
0.036431989
0.02211369
0.021097028
0.149013771
0.141687321
0.001216357
0.260557117
0.014005294
0.008094707
0.198324054
0.629861297
0.001422938
0.038292755
0.012392493
2.752592926
0.078152767
0.009672166
0.006077755
0.001114756
0.001777137
0.013948933
0.000244758
0.084560755
0.009249766
0.030699013
0.03337612
0.071213944
0.008177772
0.060755573
0.051922371
0.04063218
0.415450376
0.030538618
0.000382971
0.004446687
0.012842408
0.000743754
0.000159631
0.000332861
0.467524077
0.090443527
0.618693838
0.080824196
0.03038799
0.016813036
0.003314349
Continued
186
Table A. continued
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
0.016590211
0.007534375
0.000314305
0.019540491
0.019462651
0.037116964
0.000550249
0.00960382
0.006077179
0.008741421
0.016776534
0.000880399
0.000290718
0.005868675
1.863159286
0.008347781
0.023062089
0.006720909
0.001140307
0.135299369
0.000267543
0.0163592
0.005959881
0.007584828
0.001419836
0.030082364
0.011043906
0.001878422
0.040680783
0.032697537
0.047632886
0.023034128
0.003152153
0.022288453
0.007049097
0.00433254
0.020592043
1.138156755
0.01736615
0.062842647
0.007139218
0.000423802
0.114523119
0.018177741
0.038865187
0.025820487
0.01530923
0.016909122
0.009277357
0.005497194
0.07809036
0.007292238
0.005999488
0.014709164
0.011878852
0.001220638
0.008632112
0.037592125
0.005663282
0.001113922
0.00315622
5.177203472
0.028916636
0.006043123
0.005650405
0.000392968
0.003536285
3.505016581
0.098556803
0.007497513
0.011242937
0.103025443
0.002456052
0.000500937
0.048878458
0.013609285
0.000713941
0.033868786
0.00037179
0.003551492
0.044428048
0.000474752
0.004483787
0.001084104
0.007851699
3.022862945
0.045511504
0.01719757
0.021111349
0.0025738
0.010919246
0.007034458
0.004425099
0.000374453
0.001614225
0.005257115
0.215747698
0.150457932
0.0167211
0.011535878
0.423613382
0.013820878
0.003248284
0.003028076
0.052653747
0.003501078
0.226491886
0.005018484
0.109222468
0.001924185
0.006648237
0.007168144
0.001573446
0.008896268
0.095333364
0.010919401
0.025434037
0.748915716
0.000940251
0.052139191
0.211128504
0.004616719
0.231732945
0.001911933
0.000586207
0.110762511
0.005052671
0.002931579
0.003774015
0.002583705
0.004649203
0.005351827
0.003445407
0.125824151
0.143854252
0.023631521
0.001061799
0.325263033
0.010103635
0.009114783
0.000399859
0.278673267
0.113907766
0.001610873
0.025482913
0.695879069
0.024530059
0.008643849
0.004417447
0.021602177
0.116781449
0.004066243
0.032840827
0.033035346
0.204247634
0.134223275
0.014855045
0.097940425
0.807154228
0.02535974
0.002338299
0.002818706
0.009765059
0.000240558
0.138118791
0.083617199
0.00657685
0.001370104
0.535144542
0.018478552
0.019463565
0.037174155
0.005607757
0.0112507
0.040484908
0.25573429
0.009052823
0.00323157
0.001301029
0.014356519
0.071457204
0.002112221
0.003330636
0.02556935
0.126584048
0.25448319
0.000503811
Continued
187
Table A. continued
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
0.036233627
0.000528506
0.000522747
0.001201817
0.001292978
0.001280638
0.017288159
0.004472042
7.43077495
0.008850289
0.005881141
0.000666256
0.002064121
0.016818181
0.075804341
0.021945768
0.001181835
0.004237192
0.025362526
0.005961579
0.000857448
0.002559155
0.018592113
0.037290715
7.71568895
7.4559257
0.001681083
0.001005945
0.00031384
0.004619174
0.002873356
0.058462368
0.006491324
6.83367
0.005050771
7.5112732
Pancreatic Cancer Cell Lines

Panc-1
0.014689983
1.215614736
0.016761953
0.060569406
0.000224922
0.052034005
0.001029079
0.050880532
0.006086532
0.026948786
0.035329021
0.084373489
0.114839795
0.033656817
0.063038677
0.01656259
1.965336801
2.510819936
0.030135098
0.178421647
0.132661539
0.227935662
0.115393396
0.416784511
0.614890776
0.421903678
0.147038776
0.102008945
0.002367534
0.000896613
3.760822115
0.264434363
0.416988664
HS766T
0.029424063
0.951875153
0.007213141
0.023890217
MIA PaCa-2
0.026016543
0.170737643
0.006991831
0.004356528
HPAF-II
0.021891226
0.218718411
0.011108887
0.006321863
BxPC-3
0.054560029
0.382668146
0.005784802
0.002151561
0.128278542
0.082853392
0.152568361
0.102415662
0.001937428
0.041999072
0.075670814
0.074377407
0.11923323
0.04559057
0.055770413
0.013061064
1.113472764
2.186238461
0.03161609
0.020252717
0.109618847
0.141716512
0.15607315
0.406453515
0.736865273
1.901870835
0.73395869
0.484021242
0.000939725
0.005801776
9.582149147
0.210206731
0.38517014
0.05535581
0.003713729
0.027204065
0.062553051
0.038418142
0.15254339
0.043051702
0.014865544
0.004597732
0.454012546
0.358302514
0.002268181
0.016980739
0.028228085
0.434778369
0.195903974
0.176315727
0.452785941
1.277508216
0.327244209
0.11768722
0.102022769
0.002531811
0.063213218
0.009928174
0.040502986
0.055809211
0.052467308
0.078857242
0.012926357
0.008825643
0.005322429
0.209237025
0.644507061
0.002355114
0.003701092
0.127119219
0.008982745
0.179994418
0.300975627
0.298697707
0.086583718
0.106247262
0.000633613
0.000710354
0.599660383
0.490434906
0.174904652
0.031254965
0.358185222
0.029782996
0.143718403
0.258729367
0.351443145
0.073681958
0.126697479
0.000516091
0.002237857
0.91993971
0.528786855
0.117583475
0.006423023
1.972360915
0.37645402
0.109734195
0.094124116
0.003426986
0.032870386
0.060615086
0.043130634
0.089589329
0.048478293
0.016855891
0.005645176
0.599450993
0.5289729
0.004251714
Continued
188
Table A. continued
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
0.486750906
1.578850499
0.208011842
0.071196596
0.699443326
0.507591589
0.020922594
0.117629887
0.008302405
0.15955172
0.44866796
0.390038264
0.007240289
2.057803277
0.024433402
0.011418204
0.048000794
0.038106266
0.546206644
1.155728696
0.348605629
0.065274521
0.700339454
0.574960707
0.008321381
0.715769398
0.014061451
0.119571359
0.549599947
0.209342519
0.009241507
1.936402376
0.088664093
0.005042666
0.132358318
0.049876577
0.169812677
1.265184748
0.336704421
0.019025228
0.136718234
0.165823637
0.005522501
0.295527967
0.000427962
0.257736107
0.126600374
0.0505533
0.017006606
0.765265396
0.108435539
0.000995876
0.041085997
0.0066778
0.458434514
0.97456078
0.223547462
0.024919641
0.274594012
0.181005014
0.009275158
0.165642188
0.000708616
0.100822817
0.139861624
0.060380084
0.00041239
1.067807117
0.015955367
0.004200039
0.130966893
0.013846821
0.354744868
0.842553802
0.502601966
0.036988631
0.305880113
0.223834251
0.006113161
0.097988226
0.005549684
0.098816839
0.253051638
0.109786283
0.027582398
0.974132362
0.035594934
0.003459053
0.144220507
0.011676791
0.012733025
0.215627647
0.338061603
0.004024628
9.448907745
1.788369776
0.024845684
0.149972467
0.87253419
0.01979219
0.082452273
1.454256819
0.084867681
0.04143455
0.318227533
1.167490709
0.147506156
0.046206899
0.046255687
0.34411366
0.115293179
0.036297842
0.026105382
2.991003439
0.10598989
0.059616503
3.363711724
0.026810311
0.145450965
0.31416193
0.003257062
8.907741976
2.601441508
0.023788412
0.140219925
1.706741739
0.010725657
0.173813346
1.633027708
0.08865383
0.04303069
0.874255135
1.737092127
0.104290581
0.034778639
0.04093469
0.119008066
0.05919954
0.054503484
0.069834065
2.85193731
0.082129611
0.042031544
2.533240886
0.028767339
0.022954647
0.104214366
0.001209885
2.852457852
0.92821928
0.004336244
0.317633959
0.003376366
0.00399536
0.063388703
0.709262251
0.024232475
0.004276059
0.255560375
1.137013646
0.053001062
0.001876223
0.005467568
0.051784713
0.005196338
0.018430826
0.026173906
1.159040974
0.095090272
0.017719148
1.104991345
0.010579106
0.0584104
0.141346451
0.002364665
3.808336791
1.348737949
0.002090208
0.152413919
0.005179794
0.005549358
0.069489416
0.767925132
0.061406346
0.007400803
0.175975998
0.689367203
0.042003652
0.003857975
0.011906941
0.084178585
0.00382292
0.012933007
0.018848004
0.927787343
0.070300734
0.01749488
1.278370985
0.01232452
0.065666584
0.218977223
0.002491766
4.079556212
1.056971678
0.002820906
0.297243099
0.001180531
0.005533284
0.038273409
0.858795013
0.023827596
0.011686354
0.620078262
0.990919124
0.080452341
0.008251306
0.011735814
0.179638965
0.004344772
0.015347987
0.007130457
0.989493558
0.052004426
0.015117734
1.718137168
Continued
189
Table A. continued
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
4.292721475
0.040522207
0.004581836
0.260091484
0.655079574
1.355103052
0.038550184
0.135599012
0.041367084
0.006967067
0.022761289
0.990850682
0.000494714
0.047453443
1.84510839
0.146796306
0.004273995
0.279932824
0.008739174
0.06800634
0.034389253
0.160079899
0.850694817
0.252732494
0.076475454
0.03298394
0.023889021
0.036697614
0.032031431
0.111326509
0.059067365
0.969931703
0.064979077
0.789539894
1.592553202
0.887603689
0.056302296
0.090124369
0.002650681
0.027667777
0.238862961
0.130988455
0.034644114
4.045403391
0.183169356
0.144371669
2.960371576
0.036931298
0.000275392
0.246877587
0.354244723
1.915921922
0.045445784
0.051358247
0.020137666
0.00378194
0.031557665
0.837172757
0.000790884
0.021268208
1.72689668
0.121602835
0.000211521
0.19608088
0.002933663
0.057225353
0.035980568
0.116090938
0.798509662
0.263711843
0.114664068
0.049564699
0.042377411
0.027289699
0.180881626
0.077604266
0.022395129
1.538739241
0.035714761
0.65826882
1.452835408
0.958754779
0.021898354
0.093399509
0.000683253
0.01048616
0.213643565
0.089787491
0.031304878
1.463522849
0.162622273
0.06813804
0.780075987
0.0187605
0.002125923
0.104452474
0.166671816
0.110267655
0.014733087
0.02634575
0.02994192
0.262315156
0.004548506
0.138702103
1.004394177
0.020251633
0.001520406
0.119490603
0.205001102
0.231476729
0.041729431
0.061940274
0.005384869
0.001022685
0.005390234
0.199450075
0.013292222
0.269200234
0.045282316
0.014359502
0.316402756
0.078702216
0.001611783
0.049227783
0.001402907
0.008937955
0.020190355
0.098589347
0.186259262
0.108335545
0.025198158
0.003247903
0.003848826
0.002042525
0.033454233
0.039796147
0.038861963
0.532845457
0.014641798
0.317596423
0.835331397
0.828483327
0.033257973
0.015880285
0.03118981
0.001122817
0.016070304
0.01310991
0.054532385
0.211119474
0.110007674
0.012730502
0.004510791
0.016159457
0.001752782
0.00901883
0.029614213
0.007794791
2.387781077
0.027999581
0.331164187
0.619911456
0.802428954
0.009822807
0.010901066
0.000248049
0.001033269
0.074683848
0.024510336
0.006601834
0.78508843
0.065926992
0.0331909
0.005814767
0.104687063
0.01919638
0.008154661
0.993658536
0.023597586
0.021441046
1.208414348
0.015256478
0.003940443
0.09496612
0.255693204
0.303297649
0.047340286
0.046744444
0.006809345
0.004195416
0.013908231
0.213728502
0.000185383
0.01846378
0.370671916
0.090456546
0.000196573
0.073245134
0.003093878
0.007352174
0.008971872
0.100347336
0.305890291
0.13836186
0.047565446
0.021062109
0.010305503
0.003501138
0.033461971
0.034017884
0.034669749
0.925524906
0.016832949
0.347606923
0.412348544
0.908724713
0.050340268
0.006715506
0.00023973
0.011002805
0.097302266
0.012208246
0.011534184
1.243551899
0.077905539
0.044720735
Continued
190
Table A. continued
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
0.567789104
2.886561712
0.013737255
1.763449876
0.160294804
1.432595256
33.89899434
0.052592525
0.077414953
0.012564025
0.91047264
0.007647747
0.098836523
0.059670951
8.069417694
1.785009892
0.451087299
0.120233103
1.025481218
0.024187544
0.149841959
0.003877702
0.018270679
0.017816436
0.153397999
0.051986779
0.104068732
0.023883425
0.082441557
0.105960985
0.675010603
0.057561642
0.017007755
0.180730584
0.085237794
0.041030031
0.245108695
4.858343745
0.057981203
0.38600771
0.044254472
0.063360326
1.195419573
0.227123292
0.178113845
0.002140884
2.190982209
8.189062333
0.000825324
1.834023684
0.160347389
1.335762733
37.57524926
0.04161224
0.053718032
0.012226263
1.174366948
0.012209909
0.063932188
0.050966065
11.23286292
1.479762116
0.934878166
0.131966191
1.043302319
0.041476135
0.103966797
0.004875057
0.01369654
0.033019058
0.145895245
0.26248698
0.167242193
0.0438661
0.083705386
0.006719787
0.536266958
0.124495351
0.024180339
0.175827821
0.085136186
0.074029945
0.244671466
2.965308631
0.101093964
0.294014104
0.016023418
0.051234796
1.758591949
0.178744192
0.221228824
0.005067129
0.071559696
1.49356401
0.010110881
0.575726119
0.050400018
0.499945095
15.35388741
0.029261267
0.006388538
0.000366726
0.178871082
0.001356812
0.017067506
0.013181479
0.793942026
0.635122845
0.112668964
0.011870171
0.283351073
0.051919708
0.047588856
0.002679534
0.005678606
0.003351322
0.08823957
0.198088642
0.184939855
0.01085596
0.044308078
0.017487757
0.079293838
0.043526628
0.002229517
0.023349348
0.018194769
0.020333594
0.057031412
0.935444567
0.029696013
0.078050845
0.01091732
0.005800438
0.292340288
0.049171053
0.084231465
0.011269436
0.372834965
2.320790137
0.037196239
1.387842228
0.092224672
0.65931649
21.84227266
0.038537013
0.012474161
0.019008237
0.136685259
0.003122242
0.020759789
0.008854049
1.336135729
0.412873884
0.057055749
0.014735763
0.290716844
0.035676368
0.051919528
0.001148097
0.001269134
0.014872284
0.030933172
0.194905346
0.141519972
0.002493738
0.069132867
0.007425259
0.121553941
0.005133029
0.006394428
0.055298305
0.04122325
0.015939151
0.050273574
0.87030173
0.047848336
0.100470723
0.01427224
0.006009989
0.443862142
0.032752199
0.09833161
0.002848179
0.768942017
2.539767123
0.030077943
1.08485725
0.051054333
0.73065979
22.08802038
0.047395593
0.011491992
0.005788181
0.297152561
0.006691474
0.023259949
0.018139063
2.29606617
1.056524864
0.136171455
0.021443619
0.451570523
0.02355323
0.035701988
0.002268328
0.005688168
0.007248844
0.049256493
0.183702189
0.099790357
0.0047137
0.06054962
0.047182992
0.175746422
0.026028279
0.016328502
0.059011695
0.036834277
0.017445935
0.07436676
0.907855265
0.027498138
0.112827193
0.018499688
0.005938992
0.67912414
0.082701982
0.078404932
0.007332281
Continued
191
Table A. continued
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
Tissue Type
miRNAs
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
0.325058113
0.530496842
0.738687756
0.115483974
0.203630359
1.625364815
1.265900988
0.073122924
0.001535133
0.002261099
0.004093066
0.210093156
1.011833261
0.004742757
0.213937615
0.023350448
0.002678673
0.245514829
0.164414115
0.047020708
0.104241422
0.046779737
0.031302335
0.020188689
0.072073543
0.00090068
0.283622731
0.157948273
0.011412503
0.039172094
7.343109
0.281893627
0.379431476
0.406190885
0.177133903
0.173455774
1.735458985
1.193876222
0.058831118
0.005162706
0.001432198
0.000935518
0.109012333
0.709777511
0.003843257
0.191986899
0.031589968
0.040655639
0.011812865
0.139027508
0.075951948
0.001057981
0.006165358
0.017096488
0.008611324
0.061676588
0.000585526
0.272515136
0.128951125
0.027615944
0.010394677
7.3157717
0.049669863
0.043799314
0.213998275
0.19547284
0.053469873
0.365907672
1.214366645
0.025912636
0.000723483
0.019887263
0.132356327
0.01783599
0.002495027
0.011090591
0.001363119
0.039130519
0.013409703
0.003631251
0.00311734
0.000278593
0.003163937
0.002356651
0.000158037
0.083057086
0.017209274
0.001305959
0.021429865
7.3665025
0.0852297
0.060879067
0.153064812
0.110320635
0.047276812
0.501684256
0.608903947
0.036607158
0.022811033
0.000538328
0.022520881
0.2249251
0.000728109
0.055384614
0.010228498
0.014533662
0.016815245
0.034602668
0.038895568
0.002226923
0.015082596
0.004260514
0.010283595
0.00141642
0.151554707
0.047172098
0.001597745
0.028883344
7.807472
0.128232843
0.075029524
0.20964869
0.098423915
0.133599288
0.425251792
1.383527403
0.036216971
0.009574187
0.00996237
0.027865411
0.244906797
0.002007003
0.069419689
0.005226204
0.013451676
0.007531802
0.055716202
0.017880938
0.023643783
0.008442844
0.00513895
0.002420862
0.012549004
0.000503453
0.193058398
0.05108644
0.005569692
0.029431252
7.29358315
Pancreatic Cancer Cell Lines

Mpanc-96
0.036952533
0.168434441
0.038356385
0.008537471
0.000174829
0.166182235
0.001230644
0.133895703
0.009513738
0.053536293
0.171302792
0.016879583
PL45
0.046147461
0.506043415
0.033472766
0.00425231
Panc03.27
0.050414918
0.631018199
0.010069662
0.00175498
Panc10.05
0.058756905
0.268278848
0.016953198
0.006474351
0.168128542
0.002630083
0.145748311
0.027067737
0.029966223
0.025608562
0.034339624
0.147753122
0.131633374
0.001846209
0.164683363
0.006391174
0.05206843
0.02795596
0.053395657
0.155049956
0.004253808
0.053932511
0.024245293
0.018673032
Continued
192
Table A. continued
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
MIR-030b
MIR-030c-1,2
MIR-030d
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034b
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
0.062011262
0.039219123
0.009033827
0.000854203
0.267408841
0.205097466
0.001665989
0.063408786
0.045213865
0.188753666
0.115963892
0.098097492
0.61982939
1.099089651
0.2823961
0.098420324
0.000321462
0.005703424
3.491355029
1.058946402
0.254987348
0.279687575
4.491900806
0.289961964
0.043420949
0.154611128
0.214589006
0.000937392
0.263219553
0.001590337
0.044135614
0.157991634
0.049027876
0.051848842
0.304943119
0.065948816
0.002038838
0.030340014
0.002470559
0.097926903
0.046464367
0.012699479
0.004095028
0.524550679
0.583987976
0.008048949
0.060663122
0.04149298
0.574116558
0.140884038
0.324663604
0.42247696
0.426470563
0.133210856
0.123493804
0.011159178
1.491954015
0.123116119
0.166226012
0.340818386
0.994272058
0.30807768
0.040929563
0.291118629
0.150052324
0.01202717
0.390939447
0.000888185
0.169293275
0.19505151
0.09418183
0.03095123
0.365363664
0.085047968
0.00306635
0.068892301
0.006352009
0.286958402
0.083247631
0.070005545
0.015011527
1.541344682
1.583642312
0.015446042
0.02119504
0.068289852
1.055935724
0.125929834
0.359161227
0.8038354
0.950038918
0.437269013
0.381636224
0.000738694
0.008057201
2.121551234
0.376091738
0.34865994
0.584881199
1.065213427
0.411187559
0.089796188
0.624139541
0.450931131
0.026102296
0.125706227
0.00303903
0.303131991
0.405413917
0.130357367
0.032656818
2.686196826
0.107992013
0.00808488
0.13728758
0.025357108
0.126871218
0.069763669
0.026587247
0.009415688
0.61424909
0.896325856
0.008927851
0.025974652
0.063560894
0.479453305
0.173592704
0.204928099
0.473829432
0.785650308
0.275277714
0.071499686
0.000289451
0.001427686
4.335253089
0.149210268
0.241387641
0.270575332
2.271743923
0.394759727
0.059387052
0.32750032
0.304433435
0.010506808
0.305149413
0.000553909
0.105450637
0.293680365
0.106800234
0.034803223
1.579659067
0.110787208
0.004183629
0.098841055
0.036689059
0.033720484
0.007329335
0.232766214
0.00026051
1.831185024
0.881466147
0.014452928
0.02242087
0.445634573
0.004281519
4.815274588
2.490519262
0.026446993
0.118182434
0.224746962
0.005365045
7.150509383
2.641559707
0.030317019
0.054368383
0.369011509
0.011006196
6.912206026
1.560396556
Continued
193
Table A. continued
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-127
MIR-128a
MIR-128b
MIR-129-2
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-136
MIR-137
MIR-138-1,2
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-143
MIR-144
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-151
0.002548339
0.256197902
0.061221388
0.007186142
0.049928264
0.614871383
0.021782986
0.006021247
0.337005506
1.015961385
0.027474843
0.002018879
0.004996954
0.052313644
0.000804773
0.004123797
0.004871651
1.096190905
0.064635814
0.011056063
0.531237564
0.406529533
0.019142082
0.004356065
0.076996307
0.129994913
0.075252669
0.097811443
0.013633432
0.001334299
0.001628188
0.00098652
0.075996304
0.008901521
0.74593435
0.111523958
0.00727429
0.054892736
1.047206218
0.076577882
0.00730393
0.207592765
1.088310007
0.161451256
0.006303953
0.007322967
0.115595654
0.014539955
0.053592932
0.008577804
1.837541801
0.197238429
0.014506382
1.705443629
1.136378601
0.003902893
0.0007963
0.086714861
0.234164303
0.238135658
0.005371444
0.07573007
0.002605869
0.01065173
0.000869442
0.14764824
0.004835492
0.425435262
0.224371295
0.014404252
0.174582096
1.602468524
0.087970951
0.040726214
0.54659627
1.145563967
0.157564619
0.023069372
0.045709822
0.194473437
0.023166157
0.047543108
0.025283798
1.702771702
0.128821207
0.039473548
2.76603233
2.629150119
0.047719708
0.004118003
0.301318993
0.380307295
0.771499977
0.054747979
0.085506122
0.01301952
0.007767524
0.037960124
0.501924933
0.007042375
0.293184394
0.202721951
0.021762049
0.060871563
1.190551657
0.042180579
0.014797423
0.392770156
1.037616783
0.057923967
0.011620188
0.017903168
0.173714155
0.030171176
0.026405087
0.0338066
1.483041757
0.197510153
0.019889947
1.658259418
1.459585495
0.029610789
0.002465293
0.189432033
0.33873682
0.298946084
0.03914037
0.084167304
0.007236146
0.007860235
0.006028892
0.253455736
0.013502558
0.127289566
0.033316763
0.032151126
0.492307482
0.062052067
0.045835103
0.471383191
0.095378818
0.022719156
0.001585507
0.000793618
0.009562732
0.070475351
0.085460056
0.059042252
0.025995723
0.038725417
0.002184824
0.025616001
0.022384611
0.063615762
0.182809072
0.199816215
0.02407893
0.036954197
0.750806747
0.163513598
0.001879816
0.095554228
0.005301194
0.029463508
0.027245221
0.170614896
0.683557933
0.27137656
0.079652697
0.068384716
0.004697727
0.028573859
0.018279067
0.149152516
0.26962669
0.117294252
0.041989174
Continued
194
Table A. continued
MIR-152
MIR-153-1,2
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-182
MIR-183
MIR-184
MIR-185
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
MIR-197
MIR-198
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
0.010908606
0.116845864
0.000291465
0.016089386
0.059130368
0.026068865
1.630877102
0.038337806
0.094840006
0.478874185
0.684479894
0.003844267
0.005062757
0.001163652
0.020216605
0.002948186
0.005533913
0.350408474
0.01698214
0.052658695
3.736652932
1.369599015
0.001236303
0.495837252
0.020536556
0.177735783
8.22191564
0.016848857
0.002160144
0.004350579
0.11033528
0.000381588
0.005075737
0.008016233
0.311220192
0.349769663
0.08178038
0.055734601
0.202760997
0.054823778
0.019265428
0.000418496
0.001495712
0.011332328
0.021096801
0.013413417
0.002953036
0.013117986
0.043714602
0.022451125
0.049510131
1.254717928
0.027194356
0.534222842
1.151945186
0.693917026
0.021046885
0.012186588
0.002807551
0.001602566
0.079064593
0.016055635
0.011547102
0.96448892
0.093533668
0.039967775
0.49512547
2.514929694
0.101539965
1.450978929
0.070677341
0.603460426
21.15355259
0.031719956
0.034314248
0.001443964
0.270174153
0.036009992
0.017904513
0.011840986
2.148108804
0.999916181
0.069869667
0.041444388
0.338462304
0.166206657
0.080884079
0.001202333
0.009202711
0.006684591
0.029541401
0.01066396
0.008482951
0.007917705
0.05183188
0.030012848
0.054644232
0.5227306
0.013066885
0.791623047
1.153850266
1.265761875
0.073001024
0.032858946
0.000237945
0.017670627
0.176425318
0.05243682
0.044280659
2.672581641
0.191996514
0.067355241
0.492338348
8.535493209
0.065339717
1.982502272
0.127759327
1.658551166
32.34254987
0.076784
0.013605031
0.001990637
0.829592939
0.020365182
0.083588572
0.047682147
8.184220526
2.04359742
0.325163453
0.065005834
0.946441027
0.048953235
0.118579253
0.009433799
0.032168735
0.030460333
0.139721508
0.032411806
0.010924694
0.007825175
0.030752846
0.031324108
0.028201471
1.240326739
0.052034588
0.432025454
0.84028534
0.756385556
0.037118423
0.008696932
0.000601121
0.007974997
0.090882524
0.039464963
0.010839423
1.324131331
0.091833365
0.061740361
0.34051806
3.578360453
0.044099381
2.221685677
0.102305827
0.742590734
22.02274227
0.049138384
0.011832459
0.005267192
0.385331683
0.046785968
0.054113926
0.027384014
2.770512199
1.739338488
0.180127795
0.036332784
0.476409379
0.071482937
0.049079359
0.001456757
0.01780271
0.011190317
0.066215979
Continued
195
Table A. continued
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302b
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-381
MIR-382
MIR-383
0.110905865
0.112327383
0.007593202
0.157707652
0.008407765
0.078299292
0.021283527
0.018505778
0.017382544
0.02333714
0.002463914
0.049871557
2.041175272
0.031151632
0.07795982
0.007246477
0.004937004
0.142401581
0.024454234
0.047200406
0.001089579
0.02737863
0.035246702
0.128454209
0.127528276
0.03043408
0.127470743
1.213669467
0.039135917
0.018859341
0.121730329
0.190940238
0.007904509
0.106133615
0.030691832
0.115255106
0.090568462
0.021012201
0.078936566
0.024370558
0.025930396
0.060541325
1.289958426
0.047227438
0.114396123
0.021813016
0.005051491
0.389305483
0.032544354
0.147267764
0.028130384
0.094483163
0.09626089
0.196915567
0.091999834
0.080770908
0.537801457
2.036278752
0.066620157
0.026131433
0.142014291
0.132093009
0.021832309
0.118615465
0.098439287
0.282519043
0.149821442
0.036365419
0.117106618
0.051116311
0.074527156
0.147684622
1.619070177
0.069135404
0.238114523
0.024580814
0.012497042
1.208252952
0.138415462
0.176748395
0.007656915
0.236712618
0.338815086
0.393681963
0.186578612
0.216630611
1.048298457
2.016020095
0.063305735
0.009801244
0.001189537
0.13505334
0.063909279
0.014428731
0.095752451
0.056547017
0.212813521
0.132081354
0.022461954
0.049917802
0.034354686
0.022004856
0.060940292
1.32337343
0.031733198
0.064816221
0.012525133
0.008518684
0.546026441
0.08220436
0.14511567
0.00838748
0.132575879
0.124527538
0.187664441
0.128098432
0.104742672
0.710367124
1.710536891
0.045357399
0.00997665
0.000283338
0.003686426
0.067913023
0.000994048
0.010972002
0.010169867
0.001827666
0.000294884
0.020755595
0.030074469
0.000952635
0.000294495
0.000647896
0.004980409
0.004711872
0.004071542
0.214636088
0.000750959
0.039190616
0.01010349
0.007745586
0.001095805
0.032653344
0.028584088
0.001254
0.00097759
0.003422718
0.003448936
0.011586536
0.068177264
0.631794603
0.003778768
0.13619664
0.023037879
0.313783244
0.002828137
0.051928909
0.004821966
0.009557382
0.002611058
0.051069959
0.043706699
0.022122688
0.004766586
0.112177327
0.104060108
0.005160251
0.00379323
0.012407429
0.046626114
0.053446712
0.000723768
0.00666265
0.006732531
0.026420603
Continued
196
Table A. continued
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
18S rRNA (CT)
0.000249289
0.057932835
0.01027527
0.00931013
0.013000519
7.5105845
0.000548724
0.134893204
0.022001651
0.002139775
0.009358127
8.1212795
197
0.242676955
0.06636151
0.008987201
0.034758895
7.68454695
0.000212704
0.211581768
0.036148954
0.005215917
0.04344016
7.41360115
Figure A. Heatmap of miRNA precursor expression in pancreatic samples.

A. The relative expression of 201 miRNA precursors was determined by real-time PCR;
data are presented as CT. Unsupervised hierarchical clustering was performed on a
subset of the entire data set; data are unfiltered. A median expression value equal to one
was designated black; red increased expression; green, reduced expression; grey,
undetectable expression. B. Dendrogram representing the results of hierarchical
clustering analysis of the miRNA precursor expression pattern in 47 samples. Sample
include primary pancreatic tumors (yellow, N = 28), normal pancreatic tissues (blue, N =
6), chronic pancreatitis (orange, N = 4) and pancreatic cancer cell lines (turquoise, N = 9).
198
APPENDIX B
199
miRNAs
PANC-1
Miapaca-2
PL45
Mpanc-96
hsa-let-7a
864.050
610.148
692.112
876.530
hsa-let-7b
1474.656
1963.588
2611.949
2520.782
hsa-let-7c
44.810
68.821
97.517
114.943
hsa-let-7d
5.421
93.119
43.893
85.019
hsa-let-7e
24.693
28.415
47.948
32.652
hsa-let-7f
0.000
0.000
0.000
0.000
hsa-let-7g
420.650
442.835
316.674
1081.594
hsa-let-7i
118.281
393.891
63.906
560.419
hsa-miR-001
213.734
0.000
0.000
0.000
hsa-miR-007
79.325
231.578
258.703
220.865
hsa-miR-010a
3098.537
526.233
710.944
3555.518
hsa-miR-010b
208.210
2.341
7.859
155.023
hsa-miR-015a
136.041
194.730
118.999
175.215
hsa-miR-015b
5759.848
5761.402
5575.903
3631.885
hsa-miR-016
3475.243
3487.545
3978.712
3726.583
hsa-miR-017-3p
8.642
13.903
10.035
37.234
hsa-miR-017-5p
766.778
746.268
402.184
695.511
65.312
124.656
25.865
78.355
hsa-miR-018
hsa-miR-018b
0.000
0.000
0.000
0.000
hsa-miR-019a
977.282
2804.102
930.467
2128.957
hsa-miR-019b
5694.475
9111.486
5122.005
11294.600
hsa-miR-020
7364.867
10422.994
5679.185
12659.767
29.127
32.889
54.914
39.529
hsa-miR-021
2830.742
2438.259
2273.815
4791.171
hsa-miR-022
72.957
81.611
23.991
45.179
hsa-miR-023a
47.623
60.197
84.017
55.265
hsa-miR-023b
86.425
30.806
23.786
310.926
hsa-miR-020b
hsa-miR-023bN
hsa-miR-024
hsa-miR-025
2158.286
277.655
590.804
4914.756
15456.092
15628.700
11551.171
23228.328
712.021
739.761
659.609
934.655
hsa-miR-026aN
2092.323
944.888
1946.820
5468.540
hsa-miR-026b
1285.701
864.814
1327.705
3368.339
573.340
175.721
0.000
1568.819
1480.905
2334.120
1474.833
1526.871
hsa-miR-026bN
hsa-miR-027a
hsa-miR-027b
209.614
37.791
72.515
733.133
1917.231
336.787
597.086
6020.465
hsa-miR-028
486.117
299.220
302.150
499.902
hsa-miR-029a
1835.877
2770.324
2476.119
3207.799
hsa-miR-027bN
Continued
Table B. mature miRNA expression in pancreas cell lines.

Data are presented as copies of miRNA per cell.
200
Table B. continued
hsa-miR-029b
30.828
117.666
37.678
49.756
hsa-miR-029c
115.697
143.373
157.860
228.731
hsa-miR-030a-3p
2180.979
1887.253
1471.714
1025.028
hsa-miR-030c
6212.199
3016.941
3929.825
4837.394
hsa-miR-030d
510.943
132.479
442.222
940.854
hsa-miR-030e-3p
346.787
137.825
103.274
207.163
hsa-miR-031
14804.605
7.356
26384.031
14512.570
hsa-miR-032
49.803
125.343
33.579
41.937
hsa-miR-033
1.396
10.720
3.637
0.000
hsa-miR-034a
12.535
0.000
0.000
1.043
hsa-miR-034aN
49.334
0.000
14.296
0.000
hsa-miR-034b
1.687
7.955
0.000
0.000
hsa-miR-092N
6603.685
6110.632
4414.971
8329.002
hsa-miR-093
6586.754
9209.706
5769.697
7367.281
hsa-miR-095
8.873
23.383
31.929
28.131
hsa-miR-096
6653.265
3957.866
7663.497
4879.448
hsa-miR-098
85.958
107.388
110.607
85.914
hsa-miR-099a
5335.978
3.656
975.095
1261.087
hsa-miR-099b
403.800
358.360
380.070
298.054
1313.591
hsa-miR-100
6756.446
0.000
1057.410
hsa-miR-101
2.796
10.780
8.918
8.063
hsa-miR-103
492.989
433.180
284.603
513.420
hsa-miR-105
369.745
186.663
110.401
244.717
hsa-miR-106a
1123.657
510.667
707.918
912.946
hsa-miR-106b
890.556
1546.384
570.629
1003.441
hsa-miR-107
0.000
2.155
0.000
1.869
hsa-miR-108
0.000
0.000
0.000
0.000
hsa-miR-122a
0.000
0.000
0.000
0.000
hsa-miR-124a
0.000
0.000
0.000
0.000
hsa-miR-125a
2895.833
1984.226
3235.627
2885.609
hsa-miR-125b
2495.930
0.000
740.942
858.086
827.387
1208.406
256.223
1151.908
hsa-miR-126*
113.682
160.048
45.041
159.173
hsa-miR-127
1952.821
0.000
0.000
0.000
hsa-miR-128a
0.000
0.000
0.000
0.000
hsa-miR-130a
246.117
301.649
97.281
3.154
hsa-miR-130aN
576.053
815.060
200.421
6.888
24.032
6.581
2.693
23.622
hsa-miR-132
536.345
352.690
108.404
226.632
hsa-miR-133a
51.221
0.000
4.624
0.000
hsa-miR-134
328.861
0.000
0.000
0.000
hsa-miR-126
hsa-miR-130b
Continued
201
Table B. continued
hsa-miR-135a
9.756
18.647
15.274
21.882
hsa-miR-135b
83.673
402.734
540.723
499.139
hsa-miR-139
0.000
0.000
2.053
0.000
hsa-miR-140
419.579
1107.229
136.013
616.546
hsa-miR-141
5.253
2.603
318.910
121.750
hsa-miR-142-3p
0.000
0.000
0.000
0.000
hsa-miR-142-5p
4.454
2.571
2.202
8.790
hsa-miR-143
0.000
0.000
0.000
0.000
hsa-miR-145
1.080
0.000
8.260
6.709
hsa-miR-146
2022.389
241.074
261.745
1268.557
225.030
22.697
104.692
66.056
hsa-miR-147
0.000
0.000
0.000
0.000
hsa-miR-148a
7.923
19.597
16.990
0.000
hsa-miR-148b
109.169
60.215
31.270
91.221
hsa-miR-149
467.903
425.551
237.590
389.086
hsa-miR-150
5.302
10.623
11.357
418.904
hsa-miR-152
307.416
228.562
121.006
203.444
hsa-miR-152N
282.147
190.061
66.664
110.735
hsa-miR-153
0.000
0.000
0.000
0.000
hsa-miR-154
0.000
0.000
0.000
0.000
hsa-miR-154*
0.000
0.000
0.000
0.000
509.124
10.975
223.197
322.275
hsa-miR-146b
hsa-miR-155
hsa-miR-181a
99.637
0.000
59.778
505.728
hsa-miR-181b
713.205
105.385
415.634
2092.094
hsa-miR-181c
12.595
2.880
9.466
33.485
hsa-miR-181d
266.629
45.062
197.154
602.723
hsa-miR-182*
7.227
5.143
8.097
2.959
hsa-miR-183
245.306
401.057
150.450
243.460
hsa-miR-184
15.522
0.000
0.000
0.000
hsa-miR-186
1328.437
2129.946
618.104
1293.600
hsa-miR-187
135.515
11.274
0.000
0.000
hsa-miR-188
383.579
521.567
629.668
397.911
31.289
hsa-miR-188b
18.017
15.545
16.555
hsa-miR-190
0.000
0.000
0.000
0.000
hsa-miR-191
6749.922
2755.005
1256.139
3624.652
hsa-miR-192
107.284
190.029
72.533
14804.427
hsa-miR-193
200.340
130.367
54.820
54.030
2.319
0.000
1.831
0.000
4032.189
hsa-miR-193b
hsa-miR-194
56.171
42.884
29.659
hsa-miR-195
260.372
172.332
165.696
190.578
hsa-miR-196a
2020.987
24.667
12.565
1709.624
Continued
202
Table B. continued
hsa-miR-196b
0.000
0.000
0.000
140.840
822.094
825.354
367.643
501.897
hsa-miR-199a
0.000
0.000
0.000
0.000
hsa-miR-199a*
0.000
0.000
3.487
0.000
hsa-miR-199b
0.000
0.000
0.000
0.000
hsa-miR-199-s
0.000
0.000
0.000
0.000
hsa-miR-200a
6.694
0.000
470.674
1001.577
hsa-miR-200a*
0.000
0.000
1.408
14.060
hsa-miR-200b
15.266
8.503
1433.556
2756.762
hsa-miR-197
hsa-miR-200bN
7.323
3.151
651.476
1247.651
hsa-miR-200c
36.863
32.998
11833.947
4445.524
hsa-miR-200cN
26.774
10.587
12003.373
4263.624
hsa-miR-202
0.000
0.000
0.000
0.000
hsa-miR-202*
0.000
0.000
0.000
0.000
hsa-miR-203
9.644
0.000
422.577
16.102
hsa-miR-204
0.000
0.000
0.000
4.077
hsa-miR-205
0.000
0.000
233.410
0.000
hsa-miR-206
4.067
8.670
0.000
0.000
hsa-miR-208
0.000
0.000
0.000
0.000
hsa-miR-210
191.115
109.303
84.243
393.737
hsa-miR-210N
408.362
173.477
201.879
818.525
hsa-miR-211
0.000
1.738
0.000
0.000
hsa-miR-212
147.366
84.625
80.253
79.186
hsa-miR-213
31.440
0.000
10.486
111.251
hsa-miR-214
9.084
0.000
11.213
6.801
hsa-miR-215
14.019
0.000
0.000
55.928
hsa-miR-216
0.000
0.000
0.000
0.000
hsa-miR-217
0.000
1.014
4.797
0.000
hsa-miR-218
40.258
7.585
9.284
0.000
hsa-miR-219
0.000
0.000
0.000
0.000
hsa-miR-220
0.000
0.000
0.000
0.000
hsa-miR-221
1684.751
5026.917
2247.497
1510.636
hsa-miR-222
10090.208
26850.543
14378.551
15557.420
hsa-miR-223
5.059
6.392
0.000
12.204
hsa-miR-296
0.000
9.428
3.075
3.386
hsa-miR-299
0.000
0.000
0.000
0.000
hsa-miR-299-3p
0.000
0.000
0.000
0.000
289.144
530.584
138.497
245.915
hsa-miR-302a
0.000
0.000
0.000
0.000
hsa-miR-302a*
0.000
0.000
0.000
0.000
hsa-miR-302b
11.686
6.143
14.718
2.928
hsa-miR-301
Continued
203
Table B. continued
hsa-miR-302c
0.000
0.000
0.000
14.714
hsa-miR-302c*
0.000
0.000
0.000
0.000
hsa-miR-302d
0.000
0.000
0.000
0.000
hsa-miR-320
639.095
2664.822
531.259
2487.994
hsa-miR-321
7560.518
3685.384
3385.922
6463.011
hsa-miR-323
30.810
0.000
0.000
0.000
hsa-miR-324-3p
578.447
587.352
150.425
426.858
hsa-miR-324-5p
213.037
309.322
34.634
177.638
1.707
1.227
2.309
1.656
hsa-miR-325
hsa-miR-326
0.000
1.948
0.000
3.732
hsa-miR-328
81.036
157.912
57.546
191.665
hsa-miR-329
0.000
0.000
0.000
0.000
hsa-miR-330
9.699
17.584
4.314
11.096
hsa-miR-331
1741.754
1549.752
1247.694
1732.790
hsa-miR-335
154.016
0.000
74.827
33.366
hsa-miR-337
0.000
0.000
0.000
0.000
hsa-miR-338
0.000
11.127
9.954
0.000
hsa-miR-339
28.274
48.238
33.163
38.287
hsa-miR-340
93.409
87.527
42.445
65.627
hsa-miR-342
1074.575
0.000
0.000
12.481
hsa-miR-345
218.322
512.148
26.062
158.712
hsa-miR-346
5.678
0.000
5.614
7.678
hsa-miR-361
166.953
121.277
124.104
276.048
hsa-miR-362
8.808
15.993
10.860
13.851
hsa-miR-363
0.000
0.000
0.000
0.000
hsa-miR-365
876.765
355.292
346.851
478.339
hsa-miR-367
0.000
0.000
0.000
0.000
hsa-miR-368
0.000
0.000
0.000
0.000
hsa-miR-369
0.000
0.000
0.000
0.000
hsa-miR-369-5p
23.844
0.000
0.000
0.000
hsa-miR-370
480.461
42.935
27.846
54.579
hsa-miR-371
0.000
0.000
0.000
0.000
hsa-miR-372
0.000
0.000
0.000
0.000
hsa-miR-373
0.000
0.000
0.000
0.000
hsa-miR-374
1048.932
1208.292
381.134
1904.360
hsa-miR-375
10.015
28.024
297.572
14.582
hsa-miR-376a
454.336
0.000
0.000
5.407
hsa-miR-376b
0.000
0.000
0.000
0.000
hsa-miR-377
4.017
0.000
0.000
0.000
hsa-miR-378
66.322
82.101
17.322
42.386
hsa-miR-379
87.029
0.000
0.000
7.725
Continued
204
Table B. continued
hsa-miR-380-3p
0.000
0.000
0.000
0.000
hsa-miR-380-5p
13.915
0.000
0.000
0.000
hsa-miR-381
0.000
0.000
0.000
0.000
hsa-miR-382
657.786
0.000
0.000
4.158
hsa-miR-383
0.000
0.000
2.547
0.000
hsa-miR-384
0.000
0.000
0.000
0.000
hsa-miR-409-5p
3.894
0.000
0.000
0.000
hsa-miR-410
0.000
0.000
0.000
0.000
hsa-miR-412
0.000
0.000
0.000
0.000
hsa-miR-422b
57.130
25.544
14.357
20.773
hsa-miR-423
434.353
300.457
231.107
242.732
hsa-miR-425
286.101
96.894
23.421
68.294
hsa-miR-429
0.000
0.000
71.396
126.156
hsa-miR-431
211.319
319.630
348.651
275.681
hsa-miR-432
2.803
0.000
0.000
0.000
hsa-miR-432*
0.000
0.000
0.000
0.000
hsa-miR-433
94.334
0.000
0.000
0.000
hsa-miR-448
0.000
0.000
0.000
0.000
hsa-miR-449
7.233
0.000
9.279
6.456
hsa-miR-450
0.000
0.000
0.000
0.000
hsa-miR-451
0.000
0.000
0.000
0.000
hsa-miR-452
0.000
0.000
0.000
3.134
hsa-miR-452*
5.756
0.000
13.718
131.110
hsa-miR-453
0.000
0.000
0.000
0.000
hsa-miR-485-3p
14.821
0.000
0.000
0.000
hsa-miR-485-5p
0.000
0.000
0.000
0.000
hsa-miR-488
0.000
0.000
3.197
0.000
hsa-miR-489
0.000
4.417
0.000
22.000
hsa-miR-490
0.000
0.000
2.628
1.634
hsa-miR-491
32.483
0.000
49.550
15.174
hsa-miR-492
1.194
0.000
2.088
1.031
hsa-miR-493
0.000
0.000
0.000
0.000
hsa-miR-494
0.000
0.000
0.000
0.000
hsa-miR-495
2.887
0.000
0.000
0.000
hsa-miR-496
2.759
3.250
1.830
2.165
hsa-miR-497
2.028
0.000
0.000
0.000
hsa-miR-498
0.000
0.000
0.000
0.000
hsa-miR-499
0.000
0.000
0.000
0.000
hsa-miR-500
1.027
9.896
0.000
0.000
hsa-miR-501
35.130
82.369
1.463
1.410
hsa-miR-502
32.843
30.164
6.356
1.690
Continued
205
Table B. continued
hsa-miR-503
3.298
0.000
1.836
0.000
hsa-miR-504
0.000
0.000
0.000
0.000
hsa-miR-505
0.000
0.000
0.000
0.000
hsa-miR-506
0.000
0.000
0.000
0.000
hsa-miR-507
0.000
0.000
0.000
0.000
hsa-miR-508
0.000
0.000
0.000
0.000
hsa-miR-509
0.000
0.000
0.000
0.000
hsa-miR-510
0.000
0.000
0.000
0.000
hsa-miR-511
2.567
0.000
0.000
1.062
hsa-miR-512-3p
1.717
1.805
2.120
1.647
hsa-miR-512-5p
0.000
0.000
0.000
0.000
hsa-miR-513
0.000
0.000
0.000
0.000
hsa-miR-514
0.000
0.000
0.000
0.000
hsa-miR-515-3p
0.000
0.000
0.000
0.000
hsa-miR-515-5p
0.000
0.000
0.000
0.000
hsa-miR-516-3p
960.116
1168.878
863.947
1079.079
hsa-miR-516-5p
0.000
0.000
0.000
0.000
hsa-miR-517*
0.000
0.000
0.000
0.000
hsa-miR-517a
0.000
0.000
0.000
0.000
hsa-miR-517b
0.000
0.000
0.000
0.000
hsa-miR-517c
3.523
0.000
0.000
0.000
hsa-miR-518a
0.000
0.000
0.000
0.000
hsa-miR-518a-2*
0.000
0.000
0.000
0.000
hsa-miR-518b
8.894
7.300
1.703
9.942
hsa-miR-518c
0.000
0.000
0.000
0.000
hsa-miR-518c*
0.000
0.000
0.000
0.000
hsa-miR-518d
0.000
0.000
0.000
0.000
hsa-miR-518e
0.000
0.000
0.000
0.000
hsa-miR-518f
9.541
10.827
8.377
10.899
hsa-miR-518f*
0.000
0.000
0.000
0.000
hsa-miR-519a
0.000
0.000
0.000
0.000
hsa-miR-519b
0.000
0.000
0.000
0.000
hsa-miR-519c
0.000
0.000
0.000
0.000
hsa-miR-519d
0.000
0.000
0.000
0.000
hsa-miR-519e
0.000
0.000
0.000
0.000
hsa-miR-519e*
0.000
0.000
0.000
0.000
hsa-miR-520a
0.000
0.000
0.000
0.000
hsa-miR-520a*
0.000
0.000
0.000
0.000
hsa-miR-520b
0.000
0.000
0.000
0.000
hsa-miR-520c
0.000
0.000
0.000
0.000
hsa-miR-520d
0.000
0.000
0.000
0.000
Continued
206
Table B. continued
hsa-miR-520d*
0.000
0.000
0.000
0.000
hsa-miR-520e
0.000
0.000
0.000
0.000
hsa-miR-520f
0.000
0.000
0.000
0.000
hsa-miR-520g
0.000
0.000
0.000
0.000
hsa-miR-520h
0.000
0.000
0.000
0.000
hsa-miR-521
0.000
0.000
0.000
0.000
hsa-miR-522
0.000
0.000
0.000
0.000
hsa-miR-523
0.000
0.000
0.000
0.000
hsa-miR-524
0.000
3.378
0.000
0.000
hsa-miR-524*
0.000
0.000
0.000
0.000
hsa-miR-525
0.000
0.000
0.000
0.000
hsa-miR-525*
0.000
0.000
0.000
0.000
hsa-miR-526a
5.233
0.000
0.000
3.382
hsa-miR-526b
0.000
0.000
0.000
0.000
hsa-miR-526b*
0.000
0.000
0.000
0.000
hsa-miR-526c
0.000
0.000
0.000
0.000
hsa-miR-527
0.000
0.000
0.000
0.000
mmu-miR-129-2
0.000
0.000
0.000
0.000
mmu-miR-137
9.284
90.286
0.000
0.000
mmu-miR-224
0.000
0.000
0.000
161.951
mmu-miR-424
0.000
0.000
0.000
0.000
miRNAs
HS766T
CaPan-1
SW1990
HPNE
hsa-let-7a
1230.081
1343.353
1263.019
605.962
hsa-let-7b
1870.761
6985.835
8055.159
4187.560
hsa-let-7c
107.477
157.190
297.774
114.545
hsa-let-7d
78.436
247.065
133.216
28.820
hsa-let-7e
45.127
35.538
88.329
30.823
hsa-let-7f
0.000
121.826
71.422
5.908
hsa-let-7g
1161.567
3031.906
873.430
282.382
hsa-let-7i
493.237
544.535
420.083
80.513
hsa-miR-001
0.000
15.051
0.000
0.000
hsa-miR-007
1012.601
937.597
48.221
14.883
hsa-miR-010a
347.279
1954.989
714.767
1394.986
hsa-miR-010b
35.477
86.905
21.067
42.125
hsa-miR-015a
332.178
301.383
296.721
80.922
hsa-miR-015b
4744.868
7517.884
4741.230
1200.995
hsa-miR-016
2512.889
4131.638
23216.771
14016.092
hsa-miR-017-3p
25.878
55.282
21.189
9.003
hsa-miR-017-5p
1380.350
752.711
1293.216
315.911
364.269
350.333
234.236
50.776
hsa-miR-018
Continued
207
Table B. continued
hsa-miR-018b
0.000
0.000
0.000
0.000
hsa-miR-019a
6318.760
1495.374
1293.129
529.616
hsa-miR-019b
23081.349
8009.110
12500.154
3190.566
hsa-miR-020
26691.162
11682.849
17932.811
2490.852
106.782
111.376
23.815
6.805
hsa-miR-021
8821.574
15409.072
4669.247
2087.704
hsa-miR-022
68.976
319.141
320.670
428.544
hsa-miR-023a
57.620
118.519
165.938
69.734
hsa-miR-023b
58.732
333.215
242.951
30.653
hsa-miR-020b
hsa-miR-023bN
537.732
2602.756
4106.748
2134.444
hsa-miR-024
12423.694
24900.704
30833.748
14084.569
hsa-miR-025
1548.002
1750.739
532.804
261.889
hsa-miR-026aN
3038.350
4458.238
2478.393
1521.919
hsa-miR-026b
2099.444
1947.564
1826.644
443.561
hsa-miR-026bN
1025.426
1313.722
1043.522
286.698
hsa-miR-027a
1768.429
3478.949
6465.401
3559.906
241.408
hsa-miR-027b
97.459
783.443
528.889
2008.269
4809.437
2516.272
454.336
hsa-miR-028
559.050
1324.563
523.289
361.597
hsa-miR-029a
8124.016
3084.855
5753.919
3515.821
hsa-miR-029b
369.113
86.659
262.013
40.482
hsa-miR-029c
644.829
455.700
302.248
219.232
hsa-miR-027bN
hsa-miR-030a-3p
19.117
1575.964
5670.309
536.095
hsa-miR-030c
2155.741
9266.575
17675.582
1176.811
hsa-miR-030d
823.568
1815.420
615.465
91.388
hsa-miR-030e-3p
156.083
414.980
604.461
144.368
hsa-miR-031
19942.068
0.000
31129.427
2122.582
hsa-miR-032
194.211
81.655
47.929
23.753
hsa-miR-033
0.000
15.118
0.000
0.000
hsa-miR-034a
2.347
81.504
171.386
65.499
108.398
512.232
1058.130
577.213
hsa-miR-034b
0.000
19.256
19.865
12.382
hsa-miR-092N
12125.994
10970.557
18541.166
2862.333
hsa-miR-093
16351.386
18053.346
7062.033
1352.865
hsa-miR-095
33.619
88.552
6.537
0.000
hsa-miR-096
12850.829
23034.217
4325.126
31648.318
hsa-miR-098
258.059
150.612
125.405
19.961
hsa-miR-099a
4416.500
167.790
3575.349
8310.965
hsa-miR-034aN
hsa-miR-099b
587.805
280.100
567.198
356.239
hsa-miR-100
4187.824
228.749
4369.825
12384.337
hsa-miR-101
41.897
41.807
36.456
5.212
Continued
208
Table B. continued
hsa-miR-103
1125.711
1005.124
731.269
hsa-miR-105
330.355
0.000
0.000
0.000
hsa-miR-106a
2434.386
2082.088
1167.924
2363.999
hsa-miR-106b
250.698
184.123
3647.757
3304.220
1315.166
hsa-miR-107
1.959
15.750
31.695
0.000
hsa-miR-108
0.000
0.000
0.000
0.000
hsa-miR-122a
0.000
0.000
2.636
0.000
hsa-miR-124a
0.000
0.000
0.000
0.000
hsa-miR-125a
3827.792
3359.685
9669.414
5131.341
hsa-miR-125b
1255.494
111.703
1958.705
13036.950
hsa-miR-126
3848.807
577.380
441.293
54.490
hsa-miR-126*
630.679
93.165
69.148
18.806
hsa-miR-127
0.000
24.931
42.675
2273.944
hsa-miR-128a
0.000
0.000
0.000
0.000
hsa-miR-130a
9.510
180.776
1111.568
221.231
hsa-miR-130aN
0.000
427.174
1795.276
397.051
hsa-miR-130b
78.839
355.509
451.201
164.759
462.927
507.215
506.850
581.839
2.454
4.241
0.000
0.000
hsa-miR-134
2.964
681.593
568.509
1222.877
hsa-miR-135a
11.286
40.137
35.974
0.000
hsa-miR-135b
292.133
1194.599
1716.795
0.000
hsa-miR-132
hsa-miR-133a
hsa-miR-139
0.000
0.000
1.739
0.000
hsa-miR-140
1617.380
272.987
554.315
1288.762
hsa-miR-141
9.082
1506.712
1204.831
0.000
hsa-miR-142-3p
5.654
11.362
5.569
0.000
hsa-miR-142-5p
4.260
10.001
3.795
10.382
hsa-miR-143
0.000
0.000
0.000
20.678
hsa-miR-145
5.650
3.945
6.095
302.601
hsa-miR-146
322.064
7358.944
373.788
88.525
65.501
456.306
130.435
34.729
0.000
0.000
0.000
0.000
hsa-miR-148a
234.763
1630.279
308.654
0.000
hsa-miR-148b
148.676
372.873
223.158
50.074
93.256
283.171
1061.771
212.812
hsa-miR-146b
hsa-miR-147
hsa-miR-149
hsa-miR-150
10.961
18.824
169.116
15.944
hsa-miR-152
628.287
523.679
465.356
1032.125
hsa-miR-152N
599.658
692.663
556.732
1135.140
hsa-miR-153
0.000
7.434
0.000
0.000
hsa-miR-154
0.000
0.000
0.000
0.000
hsa-miR-154*
0.000
0.000
0.000
8.270
Continued
209
Table B. continued
hsa-miR-155
2137.202
188.687
583.550
294.940
hsa-miR-181a
17.400
964.439
904.511
356.895
hsa-miR-181b
266.273
1637.691
2029.040
393.906
hsa-miR-181c
41.747
39.454
47.768
9.065
hsa-miR-181d
362.719
741.119
1479.025
383.596
hsa-miR-182*
24.720
13.045
7.770
2.086
hsa-miR-183
565.677
198.625
266.500
20.418
hsa-miR-184
0.000
27.011
15.719
13.788
hsa-miR-186
2176.914
3285.703
1386.103
1415.933
0.000
hsa-miR-187
0.000
0.000
0.000
hsa-miR-188
559.796
379.661
0.000
0.000
23.751
28.448
33.452
34.604
hsa-miR-190
0.000
25.343
1.004
1.287
hsa-miR-191
4498.375
7857.945
2541.623
1219.360
hsa-miR-192
285.059
20713.292
92.498
10.457
hsa-miR-193
47.319
79.671
338.336
16.534
2.857
hsa-miR-188b
hsa-miR-193b
0.000
1.705
0.000
hsa-miR-194
78.359
8254.343
41.730
4.993
hsa-miR-195
175.615
310.565
115.463
36.794
hsa-miR-196a
448.196
433.017
217.178
0.000
hsa-miR-196b
15.217
1.432
0.000
0.000
579.670
683.293
473.212
409.397
hsa-miR-199a
0.000
0.000
0.000
4.078
hsa-miR-199a*
0.000
3.552
6.243
615.044
hsa-miR-199b
0.000
0.000
0.000
2.950
95.152
hsa-miR-197
hsa-miR-199-s
0.000
0.000
0.000
1753.483
10033.353
1240.690
7.685
8.560
35.373
0.000
2.569
hsa-miR-200b
1989.668
13394.016
1726.807
0.000
hsa-miR-200bN
1193.297
11574.351
1117.805
0.000
157.598
25566.636
27898.251
5.807
13.356
21426.505
18828.799
11.024
hsa-miR-202
0.000
0.000
1.590
2.351
hsa-miR-202*
0.000
0.000
0.000
0.000
hsa-miR-203
2160.283
6149.604
2850.220
0.000
hsa-miR-204
0.000
0.000
0.000
1073.849
hsa-miR-205
0.000
12.517
1812.285
0.000
hsa-miR-206
2.954
0.000
4.148
0.000
hsa-miR-200a
hsa-miR-200a*
hsa-miR-200c
hsa-miR-200cN
hsa-miR-208
0.000
0.000
0.000
0.000
hsa-miR-210
102.257
806.164
428.513
174.681
hsa-miR-210N
263.965
2223.338
882.036
467.532
Continued
210
Table B. continued
hsa-miR-211
0.000
0.000
0.000
0.000
hsa-miR-212
126.575
78.580
144.966
112.896
hsa-miR-213
10.821
145.462
185.144
66.503
hsa-miR-214
2.098
3.894
1.564
2330.115
hsa-miR-215
6.570
44.638
0.000
2.848
hsa-miR-216
0.000
0.000
0.000
0.000
hsa-miR-217
0.000
0.000
0.000
1.520
hsa-miR-218
1.300
0.000
371.070
168.110
hsa-miR-219
0.000
0.000
0.000
28.468
hsa-miR-220
0.000
0.000
0.000
0.000
hsa-miR-221
4584.006
2795.365
11340.689
6383.703
hsa-miR-222
30537.231
14207.475
50548.416
30388.349
hsa-miR-223
1.246
12.086
6.564
7.594
hsa-miR-296
6.712
922.706
4.709
43.272
hsa-miR-299
0.000
0.000
0.000
0.000
hsa-miR-299-3p
0.000
0.000
66.344
99.348
hsa-miR-301
983.825
199.935
326.238
40.694
hsa-miR-302a
0.000
0.000
0.000
33.565
hsa-miR-302a*
0.000
0.000
0.000
0.000
hsa-miR-302b
8.425
69.915
145.936
173.138
hsa-miR-302c
0.000
0.000
0.000
31.408
hsa-miR-302c*
0.000
0.000
0.000
0.000
hsa-miR-302d
0.000
0.000
1.529
55.058
hsa-miR-320
1161.400
1832.677
2212.398
546.854
hsa-miR-321
4799.042
16652.105
9201.774
4799.142
hsa-miR-323
0.000
0.000
0.000
11.742
hsa-miR-324-3p
1235.105
369.688
422.612
131.295
hsa-miR-324-5p
1036.678
541.087
271.914
107.849
2.574
0.000
0.000
0.000
hsa-miR-325
hsa-miR-326
8.178
1.137
0.000
0.000
hsa-miR-328
224.212
192.866
377.848
102.847
hsa-miR-329
0.000
0.000
0.000
0.000
hsa-miR-330
26.528
21.571
33.648
1.029
hsa-miR-331
1206.615
4792.239
1866.307
493.060
hsa-miR-335
114.012
139.660
321.407
11.130
hsa-miR-337
0.000
0.000
0.000
0.000
hsa-miR-338
0.000
41.650
0.000
0.000
hsa-miR-339
32.684
98.825
78.484
25.924
hsa-miR-340
116.539
100.997
75.191
34.890
hsa-miR-342
7.999
485.986
14.409
243.629
hsa-miR-345
863.672
420.297
490.352
84.162
Continued
211
Table B. continued
hsa-miR-346
10.275
27.980
24.326
0.000
hsa-miR-361
208.257
305.695
309.550
143.547
hsa-miR-362
9.367
3.768
25.012
10.654
hsa-miR-363
0.000
0.000
0.000
0.000
hsa-miR-365
168.404
419.028
26.289
14.586
hsa-miR-367
0.000
3.484
0.000
21.045
hsa-miR-368
0.000
0.000
0.000
0.000
hsa-miR-369
0.000
0.000
0.000
2.792
hsa-miR-369-5p
0.000
0.000
0.000
0.000
hsa-miR-370
19.938
103.808
126.559
530.437
hsa-miR-371
0.000
0.000
0.000
0.000
hsa-miR-372
0.000
258.181
227.498
104.621
hsa-miR-373
0.000
8.521
0.000
0.000
hsa-miR-374
1766.875
795.440
769.622
424.904
hsa-miR-375
9.792
7695.302
233.070
0.000
hsa-miR-376a
0.000
4.989
0.000
379.324
hsa-miR-376b
0.000
0.000
0.000
0.000
hsa-miR-377
0.000
0.000
0.000
4.113
hsa-miR-378
47.868
105.177
28.631
0.000
hsa-miR-379
0.000
7.566
1.621
89.641
hsa-miR-380-3p
0.000
0.000
0.000
0.000
hsa-miR-380-5p
0.000
0.000
7.624
8.699
hsa-miR-381
0.000
0.000
0.000
6.121
hsa-miR-382
0.000
9.162
0.000
254.060
hsa-miR-383
0.000
4.304
6.354
10.057
hsa-miR-384
0.000
0.000
0.000
0.000
hsa-miR-409-5p
0.000
0.000
0.000
0.000
hsa-miR-410
0.000
0.000
3.078
0.000
hsa-miR-412
0.000
0.000
0.000
0.000
hsa-miR-422b
14.104
57.014
28.176
3.285
hsa-miR-423
281.004
611.035
701.210
227.045
hsa-miR-425
80.644
187.599
91.016
20.357
hsa-miR-429
233.282
868.079
0.000
0.000
hsa-miR-431
325.854
102.400
0.000
3.097
hsa-miR-432
0.000
0.000
195.799
227.988
hsa-miR-432*
0.000
1.407
231.798
0.000
hsa-miR-433
0.000
0.000
179.318
46.546
hsa-miR-448
0.000
0.000
0.000
0.000
hsa-miR-449
53.301
7.283
0.000
0.000
hsa-miR-450
0.000
0.000
0.000
3.178
hsa-miR-451
0.000
0.000
0.000
0.000
Continued
212
Table B. continued
hsa-miR-452
0.000
0.000
0.000
8.297
hsa-miR-452*
0.000
52.765
1678.851
197.182
hsa-miR-453
0.000
0.000
0.000
0.000
hsa-miR-485-3p
0.000
0.000
9.359
16.103
hsa-miR-485-5p
0.000
0.000
0.000
0.000
hsa-miR-488
0.000
0.000
0.000
0.000
hsa-miR-489
0.000
4.757
41969.654
1448.000
hsa-miR-490
0.000
1.371
5975.722
3903.870
hsa-miR-491
156.672
0.000
0.000
0.000
hsa-miR-492
0.000
43.254
38.559
3.028
hsa-miR-493
0.000
0.000
0.000
0.000
hsa-miR-494
1.281
0.000
0.000
0.000
hsa-miR-495
0.000
0.000
8.217
0.000
hsa-miR-496
0.000
3.479
5.179
7.017
hsa-miR-497
0.000
1.963
0.000
0.000
hsa-miR-498
0.000
0.000
249.153
300.940
hsa-miR-499
0.000
0.000
1162.533
331.758
hsa-miR-500
0.000
0.000
0.000
0.000
hsa-miR-501
27.548
6.703
3.427
0.000
hsa-miR-502
7.486
4.154
7.851
0.000
hsa-miR-503
0.000
0.000
0.000
6.121
hsa-miR-504
0.000
0.000
0.000
0.000
hsa-miR-505
0.000
0.000
0.000
0.000
hsa-miR-506
0.000
0.000
0.000
0.000
hsa-miR-507
0.000
0.000
0.000
0.000
hsa-miR-508
0.000
0.000
0.000
0.000
hsa-miR-509
0.000
1.460
5.563
0.000
hsa-miR-510
0.000
0.000
0.000
0.000
hsa-miR-511
0.000
0.000
0.000
0.000
hsa-miR-512-3p
7.494
0.000
0.000
6.048
hsa-miR-512-5p
0.000
0.000
0.000
0.000
hsa-miR-513
0.000
0.000
0.000
0.000
hsa-miR-514
0.000
0.000
0.000
0.000
hsa-miR-515-3p
0.000
0.000
0.000
0.000
hsa-miR-515-5p
0.000
0.000
0.000
0.000
hsa-miR-516-3p
1082.658
152.779
55.446
527.907
hsa-miR-516-5p
0.000
0.000
0.000
0.000
hsa-miR-517*
0.000
0.000
0.000
0.000
hsa-miR-517a
0.000
0.000
0.000
0.000
hsa-miR-517b
0.000
0.000
0.000
0.000
hsa-miR-517c
1.670
0.000
0.000
0.000
Continued
213
Table B. continued
hsa-miR-518a
0.000
0.000
0.000
0.000
hsa-miR-518a-2*
0.000
0.000
0.000
0.000
hsa-miR-518b
12.890
6.953
4.007
5.351
hsa-miR-518c
0.000
0.000
0.000
0.000
hsa-miR-518c*
0.000
0.000
0.000
0.000
hsa-miR-518d
0.000
0.000
0.000
0.000
hsa-miR-518e
0.000
0.000
0.000
0.000
hsa-miR-518f
9.566
2.828
1.097
2.149
hsa-miR-518f*
0.000
0.000
0.000
0.000
hsa-miR-519a
0.000
0.000
0.000
0.000
hsa-miR-519b
0.000
0.000
0.000
0.000
hsa-miR-519c
0.000
0.000
0.000
0.000
hsa-miR-519d
1.560
0.000
0.000
0.000
hsa-miR-519e
0.000
1.458
0.000
0.000
hsa-miR-519e*
0.000
0.000
0.000
0.000
hsa-miR-520a
0.000
0.000
0.000
0.000
hsa-miR-520a*
0.000
0.000
0.000
0.000
hsa-miR-520b
0.000
0.000
0.000
0.000
hsa-miR-520c
0.000
0.000
0.000
0.000
hsa-miR-520d
0.000
0.000
0.000
0.000
hsa-miR-520d*
0.000
0.000
0.000
0.000
hsa-miR-520e
0.000
0.000
0.000
0.000
hsa-miR-520f
0.000
0.000
0.000
0.000
hsa-miR-520g
0.000
0.000
0.000
0.000
hsa-miR-520h
0.000
0.000
0.000
0.000
hsa-miR-521
0.000
0.000
0.000
0.000
hsa-miR-522
0.000
0.000
0.000
0.000
hsa-miR-523
0.000
0.000
0.000
0.000
hsa-miR-524
0.000
1.442
0.000
0.000
hsa-miR-524*
0.000
0.000
0.000
0.000
hsa-miR-525
0.000
0.000
0.000
0.000
hsa-miR-525*
0.000
0.000
0.000
0.000
hsa-miR-526a
0.000
0.000
0.000
0.000
hsa-miR-526b
0.000
0.000
0.000
0.000
hsa-miR-526b*
0.000
0.000
0.000
0.000
hsa-miR-526c
0.000
0.000
0.000
0.000
hsa-miR-527
0.000
0.000
0.000
0.000
mmu-miR-129-2
0.000
0.000
0.000
0.000
mmu-miR-137
0.000
5.496
13.293
297.839
mmu-miR-224
0.000
14.446
18.831
0.000
mmu-miR-424
0.000
0.000
0.000
0.000
214
APPENDIX C
215
miRNA
H23
Lung Cancer
HOP62
H522
A549
H719
let-7a-1*
265.95
179.87
207.33
185.52
161.16
let-7a-2-P
955.49
689.95
6963.23
2285.83
2472.69
let-7a-3*
33.08
83.42
211.59
93.59
156.54
let-7b*
39.04
51.46
315.75
193.40
111.64
let-7c*
6.88
211.35
0.00
7.26
16.56
let-7d*
758.26
892.58
531.53
559.29
518.95
let-7e*
11.11
8.41
8.54
13.67
8.44
let-7f-1*
604.63
767.32
536.23
407.61
566.88
let-7f-2*
99.43
152.62
146.06
28.71
60.48
let-7g*
240.90
216.06
265.32
210.99
229.67
let-7i*
192.47
103.50
302.70
397.01
61.15
MIR-001-1
235.74
237.72
150.40
161.25
158.32
MIR-001-2
280.89
489.68
206.65
230.54
201.40
MIR-007-1
102.12
63.49
74.22
33.50
106.96
MIR-007-2
41.92
135.67
54.82
131.72
172.50
MIR-007-3
131.37
80.56
30.97
18.73
38.89
MIR-009-1-P
5597.95
3393.11
1115.78
2307.45
2675.05
MIR-009-2-P
2581.00
2242.82
1550.28
1797.72
3018.59
MIR-009-3-P
80.10
67.50
42.41
45.62
58.83
MIR-010a,b
12.71
19.45
295.67
99.21
97.38
MIR-015a
481.02
329.27
431.62
171.29
309.16
MIR-015b
1702.45
907.46
1615.58
633.01
492.64
MIR-016-1
1089.23
372.69
1273.77
562.81
658.91
MIR-016-2
1004.04
979.67
946.32
721.99
474.10
MIR-017-P
2942.14
2181.34
2119.10
1400.82
1987.48
MIR-018
3558.02
1840.52
3488.41
1989.36
3561.25
MIR-019a-P
720.25
596.47
808.46
391.81
1115.88
MIR-019b-1-P
1685.67
1948.48
534.60
913.41
1423.75
MIR-019b-2-P
7.91
252.37
7.56
7.44
0.00
MIR-020
8.33
15.47
86.39
4.59
13.58
MIR-021
7919.58
34307.94
28445.73
56698.21
28133.73
MIR-022-P
687.51
630.50
1131.34
686.01
431.19
MIR-023a
772.08
663.06
395.42
522.18
577.20
1643.49
1054.85
1133.81
2111.67
1385.47
MIR-023b-P
Continued
Table C.1. microRNA precursor expression in 37 different human cancer cell

lines. All data was generated using real-time PCR and SYBR green detection except
(*) which was assayed using TaqMan probes. Data is presented as copies of
microRNA per cell. (P) Real-time PCR was performed on the microRNA primary
precursor.
216
Table C.1. continued
MIR-024-1,2
7040.69
6254.60
7985.92
19753.19
MIR-025
1099.19
1253.20
378.54
503.76
616.32
280.92
181.99
163.98
153.29
148.84
MIR-026b
2145.20
2227.08
1214.05
1237.43
1671.01
MIR-027a, b
1910.70
1729.11
1008.48
864.06
1033.30
28.26
57.34
37.84
38.49
33.75
818.00
162.66
629.82
227.71
380.48
43.77
37.08
9.39
66.72
72.46
522.59
1376.50
507.33
425.58
487.65
MIR-026a-1,2
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
4869.47
MIR-030b
1425.69
824.97
701.24
758.20
677.09
MIR-030c-1,2
1199.30
1143.95
600.29
529.57
1072.09
MIR-030d
239.05
100.49
77.00
87.62
76.41
MIR-031-P
8055.97
7666.13
3031.34
4014.24
4909.87
MIR-032
101.94
177.39
103.16
40.81
138.36
MIR-033
17.73
34.24
19.36
11.76
4.61
MIR-034a
544.66
1046.52
336.85
415.98
583.76
MIR-034b
122.55
79.37
87.76
107.06
94.86
MIR-034c
0.00
0.00
0.00
0.00
0.00
MIR-092-1
54.63
46.45
52.81
5.84
32.67
MIR-092-2-P
457.29
2077.18
157.23
203.76
300.75
MIR-093
1197.11
879.23
394.02
593.41
603.87
MIR-095
7.57
3.93
3.12
2.80
5.47
28318.18
23105.14
14521.64
22762.64
21271.33
5020.89
6762.98
4878.86
2605.99
2730.18
MIR-099a
85.21
147.46
106.00
89.92
48.01
MIR-099b
1778.78
1931.36
2476.97
2059.50
1699.39
66.86
174.68
7120.22
1396.05
2294.07
MIR-096-P
MIR-098
MIR-100
MIR-101-1
52.98
33.33
13.28
16.77
37.39
MIR-101-2
382.15
292.62
115.17
392.38
326.67
MIR-103-1,2
4723.29
4433.55
5005.15
3179.53
3623.14
MIR-105-1,2
103.87
209.00
173.88
144.27
267.53
MIR-106a-P
85.40
488.85
56.22
74.68
110.61
MIR-106b
2137.52
2795.99
1025.55
1011.34
2039.91
MIR-107
4995.26
6507.37
7196.86
4386.27
4628.41
MIR-108
635.56
390.56
401.27
264.16
391.74
MIR-122a
119.27
44.69
50.22
67.22
99.48
MIR-124a-1,2,3
157.84
55.04
65.61
59.89
130.66
1038.26
709.12
806.27
969.24
586.48
MIR-125a
MIR-125b-1
56.32
192.33
912.40
63.85
967.47
MIR-125b-2
130.81
291.63
73.80
68.32
126.51
MIR-126
132.34
56.15
48.61
122.86
173.58
Continued
217
MIR-127
4108.54
4358.95
3771.20
4154.41
4551.32
MIR-128a
546.67
589.97
417.97
328.73
520.99
MIR-128b
81.00
92.31
50.64
91.92
74.19
MIR-129-2
6335.68
5503.13
3261.62
6108.57
7099.30
MIR-130a-P
7812.21
5720.04
3814.80
4229.86
5197.07
82.44
135.93
180.25
62.54
126.19
MIR-130b
MIR-132
36.31
28.02
36.29
18.47
23.96
MIR-133a-1,2
1086.70
2412.47
367.12
628.16
1392.66
MIR-133b
1637.94
2096.22
1127.32
1450.87
1685.24
MIR-134
2164.33
3819.51
2658.98
1733.16
1950.82
MIR-135a-1,2
131.72
19.10
20.53
109.86
18.81
MIR-135b-P
284.95
229.48
121.78
60.20
229.33
MIR-136
300.20
56.44
32.38
59.31
96.91
MIR-137*
31.37
496.85
37.78
54.93
133.17
MIR-138-1,2
28.52
55.93
12.11
20.99
59.51
380.22
428.01
398.54
238.36
317.64
MIR-139
MIR-140
2.33
6.23
0.00
1.11
0.00
MIR-141
162.30
90.81
41.16
87.45
46.51
2269.21
2887.45
1558.12
1324.71
876.08
MIR-143
381.69
296.83
163.91
220.85
193.39
MIR-144
7.59
7.56
14.05
21.42
7.86
657.81
298.35
331.74
261.49
283.33
MIR-142-P
MIR-145-P
MIR-146
27.13
16.99
52.65
18.50
10.59
MIR-147
119.02
75.31
65.69
115.36
122.04
86.97
96.69
68.42
60.80
103.86
MIR-148a-P
MIR-148b-P
795.38
454.00
374.21
488.03
706.18
MIR-149
2139.25
1537.53
1175.00
958.50
1508.87
MIR-150
708.53
597.52
368.56
347.51
483.59
MIR-151
269.79
324.81
97.88
214.02
261.14
MIR-152
53.98
90.93
63.91
45.83
41.55
MIR-153-1,2
68.99
43.96
66.13
17.66
100.98
MIR-154
64.37
48.57
55.44
20.30
52.56
MIR-155
104.58
54.75
387.31
27.19
115.75
MIR-181a,c
462.74
1586.52
788.12
45.19
327.79
MIR-181b-1,2
170.82
105.22
478.39
51.93
119.33
MIR-182
16782.47
11635.17
3098.97
3692.70
5421.11
MIR-183
422.42
502.89
86.79
68.75
201.35
MIR-184
1277.07
2593.48
254.41
4276.10
1563.48
MIR-185
4185.25
3742.88
2618.53
3127.12
4049.31
MIR-186-P
2363.83
3071.69
1342.94
1535.31
2276.32
176.31
88.73
65.65
112.54
97.52
MIR-187
Continued
218
MIR-188b-P
247.95
169.92
123.15
135.49
MIR-190
4.19
4.47
1.54
1.49
2.73
MIR-191
79.51
35.34
20.38
13.27
10.44
MIR-192
862.58
1238.62
398.72
162.03
419.74
MIR-193
461.51
213.56
195.89
121.91
203.39
MIR-194-1,2
128.92
102.70
49.43
78.38
73.32
8309.40
MIR-195-P
240.23
11048.43
9480.00
5569.01
5961.99
MIR-196a-1
333.47
273.37
318.43
342.37
372.13
MIR-196a-2
403.49
718.06
254.52
218.08
523.36
MIR-196b
1886.11
485.29
1721.86
1332.71
244.78
MIR-197
7737.92
5623.72
5846.92
6877.23
6379.99
121.41
421.77
1855.34
107.09
61.85
4603.30
3755.66
3064.37
4221.39
3742.74
MIR-198
MIR-199a-1-P
MIR-199a-2-P
388.19
892.72
205.15
329.86
448.33
MIR-199b-P
3821.25
3086.45
2398.82
2164.54
2520.14
MIR-200a-P
48724.36
103362.05
52243.79
88095.16
79423.16
MIR-200b
70.31
61.37
33.90
42.31
66.46
MIR-200c
76.02
98.52
70.05
107.34
80.79
MIR-203
50.97
57.56
24.18
37.76
4.40
MIR-204
2340.50
2050.83
1150.18
1039.84
237.25
MIR-205
151.63
42.09
23.46
8.78
4.00
MIR-206
157.92
99.63
94.43
149.39
84.66
MIR-208
168.49
101.77
42.39
75.17
71.40
MIR-210
19101.01
8854.71
11551.43
6828.08
9473.77
MIR-211
5349.76
3265.12
3061.06
3179.64
3884.94
MIR-212
495.65
372.03
581.62
250.42
554.22
MIR-213
388.37
75.83
456.26
170.08
198.73
MIR-214
2472.16
2248.12
1468.28
1950.73
2392.13
MIR-215
320.99
234.47
202.01
59.81
112.19
MIR-216
349.64
324.80
202.14
189.70
294.41
MIR-217
14.59
3.70
3.34
5.15
0.88
MIR-218-1,2
32.87
109.99
204.29
13.90
26.48
MIR-219-1,2
149.47
84.22
56.01
60.13
72.36
MIR-220
557.37
260.42
266.69
296.23
386.24
MIR-221
93.53
726.01
381.65
129.43
234.46
363.07
MIR-222-P
240.53
984.95
258.50
180.07
MIR-223
82.00
92.44
41.17
61.27
99.10
MIR-224
436.36
293.11
579.04
400.99
276.96
329.08
MIR-296
MIR-299-P
MIR-301
401.58
391.90
235.52
304.70
1718.54
1038.23
902.64
857.70
294.06
452.34
1335.66
738.04
409.57
563.00
Continued
219
MIR-302
83.93
61.08
56.91
44.35
61.45
MIR-302b
217.14
168.72
91.18
157.19
64.78
MIR-302c
208.27
111.79
159.48
190.25
151.05
MIR-302d
245.29
173.45
140.30
167.43
196.37
MIR-320
457.64
508.20
244.78
328.84
342.20
MIR-321
9128.86
25372.00
4610.81
4473.53
5045.22
MIR-323
MIR-324-P
MIR-325
MIR-326-P
702.28
180.75
293.73
377.52
200.13
1061.12
864.04
525.11
578.77
679.76
52.16
75.95
37.19
37.46
69.16
121.32
75.59
24.12
57.81
40.66
MIR-328
2926.73
2635.76
1361.21
2047.73
1998.69
MIR-330
647.99
502.23
405.89
342.33
218.09
MIR-331-P
626.40
605.77
319.12
412.74
695.55
MIR-335
13.65
21.22
11.49
6.96
9.01
MIR-337
764.55
1013.00
467.62
523.29
821.16
MIR-338
1296.14
824.71
601.20
698.75
853.24
MIR-339
1894.43
877.10
1113.03
847.31
913.17
MIR-340
1277.88
1113.02
303.39
387.71
775.69
MIR-342
556.25
373.72
281.70
281.32
400.09
MIR-345
2542.22
2499.85
1452.14
1474.08
2096.17
MIR-346
3166.03
2632.09
2434.64
2407.84
2923.48
MIR-361
72.23
195.27
180.72
81.02
184.98
MIR-367
48.67
12.90
21.68
14.78
24.06
MIR-368
3.20
0.00
1.67
2.56
4.74
MIR-369
0.00
0.00
0.00
0.00
0.00
MIR-370
392.03
254.26
202.93
203.30
167.93
MIR-371
2053.40
1491.35
988.15
1038.79
1354.55
MIR-372
25.94
5.48
9.64
19.80
11.66
MIR-373
415.26
270.11
168.62
212.80
316.57
MIR-374-P
6.47
36.55
78.86
24.40
52.41
MIR-375
60.68
68.16
58.33
73.47
83.37
MIR-376
594.09
75.43
332.41
88.43
21.39
MIR-377
210.11
322.03
86.72
103.31
235.85
MIR-378
144.84
123.16
44.99
30.91
65.25
MIR-379
256.34
10.46
148.76
44.74
5.89
MIR-380
119.87
6.60
68.91
2.79
43.51
MIR-381
7.49
21.78
20.95
26.01
22.36
MIR-382
40.07
27.73
31.35
31.30
28.88
MIR-383
99.97
277.46
63.06
107.91
121.73
MIR-384
6.59
0.00
16.86
5.30
2.49
926.07
731.49
546.45
636.06
791.78
MIR-422b
Continued
220
Table C.1 continued
MIR-423
425.22
588.86
234.32
248.33
MIR-424
155.90
45.19
145.28
46.28
25.17
MIR-425
306.97
141.99
154.45
119.62
139.43
miRNA
let-7a-1*
PANC-1
Pancreas Cancer
MIAPACA-2
HS766T
MPANC-96
348.79
PL45
16.80
30.16
80.27
102.05
84.19
3224.23
2598.29
485.50
435.56
832.20
38.96
3.97
22.84
105.76
61.93
let-7b*
182.96
76.51
14.52
25.13
8.60
let-7c*
0.74
0.00
0.00
0.61
0.00
let-7d*
194.21
509.85
243.10
429.99
290.02
let-7a-2-P
let-7a-3*
let-7e*
5.20
9.34
0.00
3.94
5.43
let-7f-1*
28.23
93.16
165.28
349.71
252.98
let-7f-2*
27.53
6.81
12.47
27.87
50.54
let-7g*
149.76
255.29
83.77
145.50
55.71
let-7i*
46.10
126.67
185.79
442.66
47.93
MIR-001-1
251.23
226.75
116.54
48.23
63.46
MIR-001-2
337.41
356.14
435.89
167.46
172.93
MIR-007-1
104.29
141.97
129.95
108.03
84.75
MIR-007-2
190.09
172.16
46.99
26.52
24.50
MIR-007-3
52.92
42.94
15.29
2.78
8.30
MIR-009-1-P
5105.28
3018.80
1237.39
677.78
861.29
MIR-009-2-P
6453.36
5756.35
986.62
525.86
954.43
MIR-009-3-P
93.83
100.03
7.78
5.26
15.84
MIR-010a,b
514.30
65.33
53.37
171.07
109.37
MIR-015a
387.34
328.62
86.78
123.78
76.05
MIR-015b
650.08
420.14
1187.20
485.70
938.99
MIR-016-1
338.96
460.77
553.75
304.77
244.89
MIR-016-2
1157.98
1151.18
500.66
259.69
544.29
MIR-017-P
1679.82
2033.84
1234.19
1514.81
700.23
MIR-018
1171.58
5037.96
3329.06
2620.18
706.56
MIR-019a-P
427.41
2026.17
904.64
714.08
232.12
MIR-019b-1-P
301.25
1360.53
340.09
260.51
215.90
MIR-019b-2-P
8.23
3.46
0.00
1.09
0.00
MIR-020
3.25
19.76
21.06
17.08
21.65
MIR-021
16468.89
27976.87
5043.89
7916.38
2341.17
MIR-022-P
749.33
612.63
1034.38
2528.56
215.26
MIR-023a
1158.52
1093.45
318.07
647.63
286.88
MIR-023b-P
1343.30
1527.30
482.98
707.53
570.17
MIR-024-1,2
4140.45
3128.31
3298.33
10074.36
1587.91
Continued
221
MIR-025
595.61
993.94
929.65
732.37
MIR-026a-1,2
213.56
200.13
59.50
119.08
75.06
MIR-026b
1900.16
1937.29
392.53
401.30
490.37
MIR-027a, b
1398.27
1604.13
472.12
549.12
260.12
66.18
27.90
18.22
3.04
23.26
345.25
1978.11
820.59
667.62
650.14
MIR-028
MIR-029a,c
MIR-029b-1,2
517.66
27.34
46.08
1.58
5.03
1.92
462.14
357.11
719.91
120.96
291.94
MIR-030b
1242.58
1536.38
364.69
409.69
334.30
MIR-030c-1,2
1086.79
610.22
151.54
133.75
166.60
23.98
30.84
53.45
141.11
57.46
5334.83
5125.43
2039.01
768.53
609.39
76.77
268.26
314.47
177.62
151.11
MIR-030a,e
MIR-030d
MIR-031-P
MIR-032
MIR-033
37.08
17.28
3.54
6.38
6.29
MIR-034a
146.47
393.56
124.27
84.51
123.53
MIR-034b
117.44
154.72
21.85
7.67
12.63
MIR-034c
0.00
0.00
0.00
0.00
0.00
MIR-092-1
41.15
85.43
88.37
93.50
27.73
MIR-092-2-P
616.46
430.73
71.21
21.71
42.21
MIR-093
947.82
899.78
302.75
593.54
736.90
MIR-095
13.67
11.37
4.26
0.89
8.66
22929.50
22068.04
7178.77
4269.90
7181.80
3822.24
MIR-096-P
MIR-098
4664.63
6798.12
2452.58
2121.58
MIR-099a
78.01
76.20
14.46
7.90
17.44
MIR-099b
435.56
415.90
879.21
650.58
1206.24
195.83
MIR-100
2347.78
4542.34
11.38
165.42
MIR-101-1
62.76
35.56
13.37
21.31
14.38
MIR-101-2
245.76
510.76
188.16
136.10
99.40
MIR-103-1,2
3827.33
4354.49
1896.03
1503.21
1668.69
MIR-105-1,2
252.64
268.23
74.99
61.55
136.68
MIR-106a-P
127.24
134.34
14.27
17.99
14.43
MIR-106b
894.55
2395.23
714.09
845.66
354.84
MIR-107
3102.02
4619.59
2977.95
2430.28
1731.29
MIR-108
428.70
313.32
158.55
76.86
278.99
MIR-122a
141.23
109.58
6.49
6.32
12.54
MIR-124a-1,2,3
141.37
128.07
18.05
15.05
14.47
MIR-125a
964.05
355.50
155.07
142.32
202.67
MIR-125b-1
338.68
182.28
17.19
2.62
27.89
MIR-125b-2
97.15
112.11
168.42
57.72
12.53
MIR-126
81.79
213.49
80.73
14.69
16.83
MIR-127
7629.42
7423.07
3033.12
2613.57
2857.53
Continued
222
MIR-128a
312.49
249.31
277.34
174.23
MIR-128b
180.21
131.35
55.59
32.17
27.83
MIR-129-2
8536.54
6627.53
2897.67
1307.01
2660.70
10779.62
7692.85
2076.75
1011.86
1804.38
124.56
116.06
58.71
54.40
7.93
15.48
1.07
7.31
13.20
1.73
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
337.89
737.55
714.50
303.41
205.98
153.94
MIR-133b
1784.71
1009.31
474.43
339.96
398.17
MIR-134
3577.31
5073.56
319.55
201.52
404.63
MIR-135a-1,2
118.75
141.54
46.59
258.97
10.76
MIR-135b-P
395.54
159.11
81.24
39.32
135.23
MIR-136
127.04
64.97
91.82
4.26
5.39
MIR-137*
23.11
13.12
732.14
5.15
20.71
MIR-138-1,2
71.74
99.85
15.14
3.19
1.88
MIR-139
2651.48
2297.95
397.97
203.42
256.13
MIR-140
1.84
2.94
0.00
0.00
0.00
MIR-141
MIR-142-P
MIR-143
MIR-144
144.87
68.46
42.22
38.95
59.59
4806.11
4593.64
750.51
333.19
810.57
426.73
362.91
136.39
92.43
111.77
14.48
0.83
0.00
0.00
0.00
791.29
573.19
95.47
64.08
71.19
MIR-146
28.71
10.29
3.97
5.02
4.55
MIR-147
204.40
176.46
50.63
2.59
47.94
MIR-148a-P
106.46
113.20
41.67
28.00
42.14
MIR-148b-P
463.60
347.16
162.93
189.26
114.46
MIR-149
2291.53
2196.33
594.82
227.59
314.20
MIR-150
717.58
761.04
318.83
159.78
342.11
MIR-151
228.69
343.08
40.51
72.90
45.19
MIR-152
102.30
153.79
15.02
31.76
25.82
MIR-145-P
MIR-153-1,2
75.13
132.39
50.90
306.99
6.07
MIR-154
113.29
86.90
6.08
0.99
25.27
MIR-155
99.47
530.61
29.13
46.07
79.94
MIR-181a,c
327.53
236.16
90.85
160.01
42.26
MIR-181b-1,2
178.62
71.92
25.34
73.09
90.05
2597.90
4113.66
6055.84
3821.98
1983.74
MIR-182
MIR-183
195.69
112.40
86.11
105.71
50.77
MIR-184
2133.69
1825.81
915.01
251.44
876.48
MIR-185
4174.82
3893.69
1666.88
1183.49
1828.01
MIR-186-P
1125.64
2386.55
2616.25
2133.64
1665.63
MIR-187
170.61
70.40
31.61
11.71
39.73
MIR-188b-P
267.59
281.95
34.93
15.24
23.56
Continued
223
MIR-190
9.17
2.55
0.94
0.00
5.78
MIR-191
86.46
34.80
3.67
3.73
3.38
MIR-192
679.86
622.21
220.12
57.31
140.92
MIR-193
382.66
271.51
75.82
9.09
30.67
MIR-194-1,2
107.22
99.09
21.62
16.59
22.37
10184.74
3921.09
2089.51
877.81
1542.37
MIR-196a-1
527.38
479.25
195.36
48.51
165.50
MIR-196a-2
419.99
208.52
101.33
143.21
73.38
MIR-196b
1556.51
5768.30
211.30
8447.67
815.01
MIR-197
7374.37
20361.72
3865.80
3234.06
3858.07
MIR-198
44.25
3.06
32.50
3.96
179.03
MIR-199a-1-P
4602.43
4865.89
1553.04
1223.57
2279.62
MIR-199a-2-P
464.20
472.84
151.10
58.18
126.59
MIR-195-P
MIR-199b-P
3772.77
3593.00
1356.90
458.55
984.85
MIR-200a-P
77829.22
87456.35
35921.32
17962.89
29588.61
MIR-200b
159.84
130.10
89.82
48.14
58.82
MIR-200c
231.37
166.10
20.95
6.75
63.41
MIR-203
40.63
40.31
1.36
13.18
3.06
MIR-204
2445.34
3176.55
507.60
290.60
456.56
MIR-205
25.27
40.26
4.76
1.28
66.41
MIR-206
292.28
196.19
53.63
15.28
34.04
MIR-208
180.37
157.95
41.88
23.66
22.92
MIR-210
19716.38
27549.84
2112.05
783.66
3317.95
MIR-211
4656.25
3962.70
1705.99
876.28
1596.53
MIR-212
1248.99
2553.89
326.20
218.21
125.20
MIR-213
352.55
392.45
37.89
151.21
75.97
MIR-214
2740.06
2836.55
788.21
520.13
566.40
MIR-215
76.03
129.69
155.46
148.84
286.85
MIR-216
435.20
312.39
143.03
54.73
144.02
MIR-217
13.20
16.73
9.12
1.40
2.57
MIR-218-1,2
58.13
44.94
18.72
4.75
18.01
MIR-219-1,2
56.75
104.27
11.30
32.94
13.26
MIR-220
445.07
431.98
258.20
59.70
54.95
MIR-221
158.08
757.66
559.65
292.04
212.95
MIR-222-P
307.07
492.27
524.06
295.62
327.56
MIR-223
75.12
136.83
34.79
22.46
15.57
MIR-224
245.73
253.89
133.58
408.99
186.77
MIR-296
312.41
22.74
54.89
24.76
57.00
MIR-299-P
1836.62
1500.70
233.10
209.32
202.10
MIR-301
174.26
371.17
131.33
60.20
160.48
MIR-302
54.28
77.40
7.65
52.66
39.67
Continued
224
MIR-302b
520.66
516.42
72.38
49.60
MIR-302c
253.69
258.04
57.01
65.75
45.71
MIR-302d
126.05
225.74
63.41
7.65
48.51
140.70
MIR-320
696.86
708.39
170.07
135.95
109.16
MIR-321
12134.67
7705.12
2470.84
4737.19
2037.01
MIR-323
175.48
304.13
91.09
86.67
86.08
200.66
MIR-324-P
1076.04
844.50
229.60
208.45
MIR-325
135.51
52.21
34.97
21.48
41.11
MIR-326-P
191.02
158.74
19.10
14.88
10.14
MIR-328
3172.97
4674.27
812.12
370.93
647.54
MIR-330
647.86
524.61
147.57
68.76
60.28
MIR-331-P
513.45
643.32
246.97
128.98
255.50
7.48
17.36
36.05
3.51
52.44
167.11
MIR-335
MIR-337
912.91
811.17
149.01
76.60
MIR-338
1458.57
1077.86
132.11
97.54
170.11
MIR-339
2002.03
1150.47
602.58
336.10
337.36
MIR-340
339.22
520.09
552.58
333.78
162.90
MIR-342
583.60
509.75
159.89
84.76
143.83
MIR-345
4257.07
4615.43
1006.64
333.64
882.09
MIR-346
3351.71
3227.02
3171.48
2880.92
3152.52
MIR-361
219.09
181.19
79.96
107.82
119.63
MIR-367
5.44
17.67
0.00
53.63
48.87
MIR-368
7.88
5.18
2.61
0.00
0.00
MIR-369
13.90
3.45
0.00
0.97
0.00
MIR-370
601.31
326.88
62.08
11.25
8.25
MIR-371
2705.17
1962.26
380.54
182.67
366.35
MIR-372
16.00
13.32
0.00
3.21
1.64
MIR-373
611.83
561.73
55.94
31.94
72.01
73.51
99.95
8.52
29.70
19.69
MIR-375
9.26
127.24
35.51
5.75
15.27
MIR-376
697.96
39.00
4.78
1.00
2.35
MIR-377
475.60
412.51
118.61
58.77
60.48
MIR-378
143.60
231.34
42.58
83.80
53.25
MIR-379
307.56
3.88
12.20
3.08
2.68
MIR-380
142.90
20.94
10.54
1.00
2.11
MIR-381
97.30
55.55
1.05
2.13
6.99
MIR-382
63.96
28.83
10.70
15.00
7.04
MIR-383
216.08
189.57
8.07
14.23
22.44
MIR-374-P
MIR-384
3.27
2.20
0.61
0.86
1.21
MIR-422b
801.27
785.33
243.67
156.91
234.92
MIR-423
457.69
383.86
54.05
30.00
41.45
Continued
225
MIR-424
37.06
87.89
4.59
27.30
4.46
MIR-425
120.59
34.51
66.67
37.57
18.30
miRNA
let-7a-1*
let-7a-2-P
PC3
Prostate Cancer
DU145
PPC1
TSUPR1
LNCAP
143.19
3.38
13.13
43.56
11.49
7803.95
1821.31
1279.00
270.94
1081.41
let-7a-3*
468.33
41.00
16.76
15.01
168.71
let-7b*
362.67
214.51
115.40
37.70
633.87
let-7c*
0.00
1.98
0.00
6.95
696.73
let-7d*
250.35
84.65
133.10
139.25
182.83
let-7e*
10.25
2.62
2.06
0.00
14.23
100.43
let-7f-1*
189.51
17.10
6.48
38.59
let-7f-2*
25.79
37.07
16.86
12.57
4.84
let-7g*
213.29
41.25
26.16
137.57
172.53
let-7i*
195.52
82.13
85.05
89.15
76.55
MIR-001-1
89.47
0.61
182.82
7.01
323.81
MIR-001-2
490.26
466.93
791.41
103.47
432.13
MIR-007-1
102.00
2.16
107.86
1.69
175.44
MIR-007-2
31.22
3.32
66.10
1.94
80.76
MIR-007-3
12.26
4.26
20.27
3.93
78.76
MIR-009-1-P
1421.70
1676.30
1834.68
761.10
2469.69
MIR-009-2-P
791.18
638.66
1737.68
769.99
4470.95
MIR-009-3-P
17.74
9.25
31.99
5.10
38.68
MIR-010a,b
177.69
0.00
44.03
3.81
12.53
MIR-015a
138.46
75.13
193.41
27.99
163.98
MIR-015b
801.98
526.38
440.52
192.09
700.09
MIR-016-1
692.86
213.80
444.17
1258.48
1097.20
MIR-016-2
476.06
289.82
680.06
136.66
547.78
MIR-017-P
278.14
372.46
537.63
176.51
911.49
1843.05
1591.29
838.76
2140.52
5244.02
MIR-018
MIR-019a-P
261.29
53.79
57.81
31.05
1394.49
MIR-019b-1-P
519.82
206.98
698.10
175.33
999.42
MIR-019b-2-P
2.57
0.00
0.00
0.00
4.55
MIR-020
0.99
0.00
1.94
0.00
24.87
MIR-021
27307.07
16179.01
26034.95
58978.05
11180.51
MIR-022-P
847.47
224.08
228.01
312.93
1998.11
MIR-023a
384.12
93.54
451.50
122.45
489.04
MIR-023b-P
697.38
141.92
1156.37
292.68
1639.94
MIR-024-1,2
3192.41
2824.37
6164.42
4829.63
1930.89
120.90
248.46
334.29
192.51
583.07
MIR-025
Continued
226
MIR-026a-1,2
67.86
30.10
137.99
5.53
182.86
MIR-026b
618.60
146.02
702.08
139.92
1126.24
MIR-027a, b
627.34
286.11
958.46
261.29
1245.07
39.45
MIR-028
MIR-029a,c
MIR-029b-1,2
18.74
11.17
19.72
24.97
389.26
117.98
1554.76
687.60
78.84
4.59
13.41
22.10
0.00
16.71
MIR-030a,e
101.61
42.71
204.39
42.16
386.97
MIR-030b
660.66
297.34
639.77
217.38
839.65
MIR-030c-1,2
497.20
180.53
551.17
101.24
541.49
MIR-030d
105.90
50.66
37.70
1.45
101.11
MIR-031-P
2897.58
2220.49
3777.19
1207.00
3333.38
MIR-032
84.70
63.31
362.60
86.38
172.04
MIR-033
1.29
2.64
1.94
4.14
7.67
MIR-034a
159.55
92.83
237.03
75.04
714.64
MIR-034b
30.25
30.26
43.46
18.08
129.86
MIR-034c
0.00
0.00
0.00
0.00
0.00
MIR-092-1
2.29
0.65
1.29
1.08
22.82
MIR-092-2-P
102.50
19.82
128.43
8.26
263.51
MIR-093
237.81
73.29
258.23
77.70
555.98
MIR-095
35.73
0.00
1.66
0.00
17.20
MIR-096-P
8420.23
7080.78
7725.59
4131.95
16119.55
MIR-098
3064.95
9591.90
3002.87
5470.93
11417.13
MIR-099a
50.42
24.17
13.15
18.92
113.79
MIR-099b
719.48
143.94
256.80
203.49
350.12
27721.96
10716.83
2443.09
3061.02
69.42
MIR-100
MIR-101-1
3.08
3.27
32.13
2.59
48.74
MIR-101-2
168.77
119.16
371.70
383.37
168.14
MIR-103-1,2
2255.30
870.77
2771.90
2628.59
3985.63
MIR-105-1,2
83.01
48.36
211.21
56.74
225.47
MIR-106a-P
50.46
5.49
29.26
5.54
91.88
MIR-106b
1140.59
1221.49
1037.03
1675.07
2317.89
MIR-107
4209.53
1366.77
3687.58
6232.79
4434.74
MIR-108
161.06
78.38
316.94
72.05
237.81
MIR-122a
16.74
8.61
51.57
0.90
48.63
MIR-124a-1,2,3
23.04
46.82
19.87
29.09
59.03
940.32
MIR-125a
519.27
324.47
592.44
125.99
MIR-125b-1
1639.63
572.43
199.41
247.66
12.65
MIR-125b-2
47.70
47.20
73.46
4.30
489.56
MIR-126
38.85
2.20
98.54
12.94
54.94
MIR-127
3596.37
525.31
2803.02
383.88
3797.87
MIR-128a
450.87
245.01
463.95
487.28
708.52
Continued
227
MIR-128b
59.26
22.24
53.68
15.25
107.76
MIR-129-2
3557.64
1015.30
3696.52
770.40
6436.40
MIR-130a-P
3014.93
1341.66
3706.16
925.38
4999.61
29.91
30.59
22.24
4.07
126.40
0.63
0.75
28.06
36.09
62.30
359.43
135.31
546.52
65.56
559.46
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
451.32
321.68
642.65
141.05
1117.14
1074.84
478.97
1419.85
375.11
3132.08
MIR-135a-1,2
65.92
11.93
39.76
31.24
115.13
MIR-135b-P
89.87
89.04
148.00
25.55
111.23
MIR-136
80.15
7.37
55.13
5.46
44.03
MIR-137*
73.09
0.99
12.03
420.03
24.67
6.61
3.58
12.99
0.86
50.24
MIR-139
605.63
333.74
849.31
198.32
609.30
MIR-140
0.00
0.00
0.00
0.00
1.53
MIR-141
34.75
1.89
49.50
1.37
151.39
MIR-142-P
598.68
460.07
894.10
493.80
907.11
MIR-143
117.78
48.04
184.77
35.76
350.82
MIR-144
0.00
0.00
36.75
0.00
1.15
218.53
MIR-134
MIR-138-1,2
MIR-145-P
147.38
62.53
241.61
41.59
MIR-146
19.57
0.00
11.11
0.00
7.47
MIR-147
53.80
15.65
87.62
49.44
169.96
MIR-148a-P
52.29
4.30
36.68
3.01
41.99
MIR-148b-P
372.22
135.92
358.94
13.26
636.13
MIR-149
635.84
366.13
506.15
323.25
425.02
MIR-150
221.82
454.15
296.94
339.28
539.24
MIR-151
47.71
30.94
64.82
29.58
174.25
30.27
4.37
75.59
7.27
71.16
110.48
37.16
20.92
10.97
34.50
66.54
MIR-152
MIR-153-1,2
MIR-154
15.87
6.36
3.55
2.53
MIR-155
49.88
21.75
100.50
0.00
34.19
513.58
373.99
674.73
1013.07
570.72
MIR-181a,c
MIR-181b-1,2
28.29
44.54
100.55
68.09
63.61
MIR-182
19259.56
3736.58
5289.56
2424.54
6254.87
MIR-183
2185.76
31.11
115.86
21.08
165.81
MIR-184
790.67
450.90
1090.14
308.72
1901.97
MIR-185
1520.52
444.78
2044.93
382.46
2361.75
MIR-186-P
3362.29
1415.85
1178.33
663.57
5835.59
MIR-187
15.06
25.00
44.25
3.59
57.11
MIR-188b-P
34.55
17.24
75.31
15.91
149.05
0.00
0.00
13.47
0.00
4.49
MIR-190
Continued
228
MIR-191
34.03
1.55
16.00
2.97
MIR-192
0.00
0.00
0.00
0.00
0.00
MIR-193
123.67
55.81
78.76
25.82
156.08
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
24.98
32.15
23.36
26.60
12.59
71.58
3566.94
3078.37
5740.18
1707.15
7202.21
63.86
29.78
144.96
54.71
235.03
209.86
161.48
217.71
71.71
247.93
MIR-196b
17440.37
7906.50
2621.91
6794.11
2220.79
MIR-197
11434.15
4772.09
6465.38
2466.05
3859.64
MIR-198
MIR-199a-1-P
MIR-199a-2-P
374.49
582.26
7.64
129.93
142.70
1115.10
378.34
1855.95
303.14
1808.13
150.70
122.14
151.10
25.18
206.67
MIR-199b-P
1261.58
1242.56
1802.80
485.85
1692.24
MIR-200a-P
33749.36
22042.83
26561.37
14966.42
71326.06
MIR-200b
44.61
7.59
29.23
2.77
145.67
MIR-200c
56.75
5.20
38.74
3.08
110.18
MIR-203
1.47
3.38
14.24
10.18
55.33
MIR-204
680.36
505.12
859.68
87.35
1466.30
MIR-205
23.76
5.41
57.73
9.72
58.22
MIR-206
54.13
24.04
45.58
13.47
165.32
MIR-208
15.48
26.00
63.55
35.44
84.71
MIR-210
2674.65
4916.21
5848.40
1665.02
9218.05
MIR-211
1303.52
1153.79
2765.18
899.53
4017.09
MIR-212
180.80
377.71
161.77
218.13
447.85
MIR-213
298.07
82.32
116.19
183.03
84.92
MIR-214
935.20
660.71
1158.28
430.22
1646.30
MIR-215
139.39
117.68
91.56
37.01
123.30
MIR-216
110.10
73.09
100.43
18.79
186.22
MIR-217
4.16
0.00
3.43
0.00
5.86
MIR-218-1,2
418.16
15.35
17.86
4.63
23.84
MIR-219-1,2
46.51
5.54
54.73
22.93
95.47
MIR-220
133.55
96.01
341.59
109.66
308.06
MIR-221
603.99
43.65
257.19
176.78
9.85
MIR-222-P
217.09
52.04
179.16
17.24
55.69
MIR-223
35.41
6.46
28.34
3.71
68.14
MIR-224
80.28
18.11
102.78
5.00
170.27
MIR-296
53.83
72.81
165.47
22.65
271.33
MIR-299-P
775.60
776.13
786.66
167.41
730.48
MIR-301
220.22
91.25
647.67
333.49
435.60
MIR-302
21.16
1.03
27.99
2.51
65.48
MIR-302b
89.25
10.61
128.93
6.75
85.94
Continued
229
MIR-302c
61.09
10.91
106.79
5.17
85.09
MIR-302d
49.25
21.25
65.77
5.40
134.24
MIR-320
148.34
140.07
259.58
100.77
530.67
MIR-321
4727.31
5456.91
4070.12
7669.81
11284.62
MIR-323
103.81
19.90
136.03
61.73
136.41
MIR-324-P
281.42
302.40
496.47
144.67
508.54
25.91
MIR-325
71.26
4.78
13.98
8.34
MIR-326-P
20.25
12.95
25.77
2.64
28.13
MIR-328
839.85
563.54
1160.87
422.23
2225.28
MIR-330
225.25
187.22
202.35
45.33
515.13
MIR-331-P
213.19
103.34
263.55
59.11
470.40
MIR-335
2.83
6.95
25.08
5.44
2.17
MIR-337
158.62
127.84
327.55
38.22
578.33
MIR-338
300.39
85.36
538.28
88.57
378.56
MIR-339
992.34
257.97
630.54
258.69
1047.38
MIR-340
275.40
217.03
375.50
156.23
1234.53
MIR-342
108.53
72.67
149.55
42.10
233.95
MIR-345
696.32
460.56
1135.27
411.69
1954.75
MIR-346
1130.91
325.37
824.50
242.79
1555.54
MIR-361
111.41
55.01
42.87
178.79
9.16
MIR-367
18.01
0.00
5.55
27.26
84.53
MIR-368
1.90
0.00
1.80
0.00
0.00
MIR-369
0.00
0.00
17.06
0.00
0.00
MIR-370
114.48
64.86
146.39
46.64
162.50
MIR-371
454.50
454.08
915.52
265.16
807.42
MIR-372
5.77
0.72
1.70
1.33
3.47
MIR-373
70.22
49.74
153.42
25.31
289.97
MIR-374-P
8.93
0.00
0.00
1.42
15.88
MIR-375
36.15
53.13
14.69
65.38
9.93
MIR-376
21.81
9.84
0.70
10.86
2.67
MIR-377
63.20
102.88
5.07
184.44
3.77
MIR-378
62.81
22.56
5.59
75.57
32.68
MIR-379
10.53
1.41
0.00
2.30
0.85
MIR-380
0.00
9.15
0.00
2.48
0.00
MIR-381
4.34
3.81
2.02
11.42
1.66
MIR-382
14.20
21.58
4.17
47.78
0.00
MIR-383
26.00
31.59
8.60
95.19
75.27
MIR-384
0.00
0.00
0.00
0.00
0.00
MIR-422b
319.03
183.74
365.61
91.60
787.16
MIR-423
134.69
97.50
68.78
30.63
64.90
MIR-424
128.90
38.32
79.82
30.05
51.41
Continued
230
MIR-425
miRNA
let-7a-1*
1270.22
SCC17A
38.46
176.90
32.92
Head & Neck squamous cell carcinoma

SCCD12
SCC10B
SCC17B
808.57
SCC5
97.56
47.14
85.66
9.29
114.80
2181.48
1627.60
3047.02
677.51
2725.60
48.99
50.62
15.27
40.13
104.19
let-7b*
182.72
59.10
93.54
14.10
331.79
let-7c*
0.00
0.00
2.92
0.00
38.87
let-7d*
345.83
211.88
784.53
83.32
469.00
let-7e*
4.96
11.01
1.51
12.36
8.06
99.87
58.37
106.21
21.82
147.73
let-7a-2-P
let-7a-3*
let-7f-1*
let-7f-2*
18.45
60.75
86.28
18.48
35.20
let-7g*
107.16
162.06
85.51
29.96
112.65
let-7i*
49.93
20.53
64.82
11.99
52.46
MIR-001-1
392.94
220.71
987.67
27.28
764.84
MIR-001-2
277.98
206.70
441.68
78.87
596.56
MIR-007-1
92.56
103.20
271.79
16.87
206.96
MIR-007-2
232.01
342.48
438.46
134.85
325.54
MIR-007-3
69.07
11.64
19.72
39.44
120.86
MIR-009-1-P
8684.55
20022.85
11202.45
11727.26
9435.17
MIR-009-2-P
6674.43
3045.53
10112.89
1060.81
6878.04
MIR-009-3-P
168.68
53.62
362.10
32.20
270.25
MIR-010a,b
27.62
6.59
101.83
5.02
86.28
MIR-015a
776.87
382.58
995.75
215.98
1354.60
MIR-015b
1255.74
1245.00
712.93
550.41
1381.27
MIR-016-1
755.82
544.73
532.42
278.58
1023.78
MIR-016-2
1246.09
1267.98
1776.64
1011.29
1927.71
MIR-017-P
1603.86
1489.17
2718.48
841.44
2868.79
MIR-018
1478.61
3390.66
1471.00
381.73
4950.29
454.99
1046.33
608.93
100.91
2342.99
MIR-019b-1-P
1863.56
1605.28
3111.60
1995.79
3401.75
MIR-019b-2-P
16.14
15.11
7.79
1.06
6.43
MIR-020
9.71
6.81
27.84
3.46
7.98
MIR-021
34924.79
MIR-019a-P
18734.27
24011.81
47626.61
14564.60
MIR-022-P
1070.96
559.58
1680.01
256.00
2087.32
MIR-023a
1624.96
1101.81
3336.22
746.34
1144.15
MIR-023b-P
2916.82
998.91
4747.52
517.43
4086.99
MIR-024-1,2
5952.26
8113.48
10823.30
6466.39
36802.72
MIR-025
945.74
369.86
1426.20
178.76
1076.87
MIR-026a-1,2
390.12
146.41
746.95
80.09
518.96
Continued
231
MIR-026b
2710.92
988.23
6541.30
618.02
4084.45
MIR-027a, b
3032.99
898.39
5323.47
489.58
4219.40
153.57
41.40
120.23
46.08
210.64
MIR-028
MIR-029a,c
1059.72
542.59
746.53
317.15
623.57
81.55
157.76
185.85
97.77
119.17
551.97
244.24
1164.23
40.88
751.33
MIR-030b
1657.44
653.01
3347.95
338.52
2090.38
MIR-030c-1,2
1680.65
554.29
2886.48
247.58
2648.28
38.30
23.30
169.64
1.13
74.68
MIR-029b-1,2
MIR-030a,e
MIR-030d
MIR-031-P
13347.76
7342.21
22554.51
3398.07
18361.32
MIR-032
297.89
155.64
646.36
18.45
521.43
MIR-033
54.13
15.28
140.81
2.59
57.96
MIR-034a
1180.68
1256.29
1866.80
940.88
1963.02
MIR-034b
249.32
129.02
366.22
50.76
296.73
MIR-034c
0.00
0.00
0.00
0.00
0.00
MIR-092-1
36.20
78.10
80.81
13.42
164.98
MIR-092-2-P
436.53
168.57
1234.94
82.99
707.04
MIR-093
1143.10
1900.00
2190.31
1537.15
1459.50
MIR-095
20.13
6.81
64.55
4.02
7.08
23359.90
29188.11
34457.00
14239.61
31733.46
4764.91
4042.46
10246.63
1564.73
6956.44
MIR-099a
60.48
73.72
108.94
50.51
78.87
MIR-099b
427.35
741.58
574.89
983.86
351.67
MIR-100
540.14
4523.79
223.19
404.51
216.87
46.62
58.15
195.90
25.74
248.27
MIR-096-P
MIR-098
MIR-101-1
MIR-101-2
570.40
952.04
939.28
649.54
1673.92
MIR-103-1,2
4049.09
3479.62
6695.88
1931.93
5920.25
MIR-105-1,2
540.48
174.25
799.38
102.53
542.19
MIR-106a-P
209.74
128.27
419.22
43.81
335.08
MIR-106b
1460.17
1453.89
1683.50
1242.37
2058.86
MIR-107
4244.97
4207.71
6625.55
2942.94
6387.63
MIR-108
766.01
270.31
1271.85
181.27
946.23
MIR-122a
131.01
46.89
290.76
43.68
228.04
MIR-124a-1,2,3
183.49
127.47
423.20
144.37
215.31
1188.83
542.18
2048.02
513.90
1483.59
115.51
MIR-125a
MIR-125b-1
101.68
95.04
133.72
33.12
MIR-125b-2
227.94
107.49
546.85
32.49
292.52
MIR-126
139.81
58.30
149.61
264.35
130.80
MIR-127
7650.07
8157.38
8719.52
6955.59
10030.36
MIR-128a
851.90
8021.02
941.47
3298.28
661.52
MIR-128b
242.05
592.67
303.19
541.96
318.26
Continued
232
MIR-129-2
11576.75
3555.18
22329.18
1561.83
14186.73
MIR-130a-P
11632.11
4613.82
23551.55
1716.34
14632.10
239.94
173.32
428.58
82.58
190.41
MIR-130b
MIR-132
61.16
32.87
47.40
24.91
80.16
MIR-133a-1,2
1478.76
1913.47
4147.62
746.93
2080.98
MIR-133b
1874.82
1182.64
3505.91
968.99
2782.82
MIR-134
5729.84
2114.71
9802.38
1311.28
7729.64
52.20
21.35
295.44
10.34
452.52
MIR-135b-P
448.43
261.97
778.06
145.47
709.39
MIR-136
118.44
132.40
160.18
23.63
102.57
MIR-137*
14.79
17.67
71.14
13.33
101.48
MIR-135a-1,2
MIR-138-1,2
MIR-139
54.06
36.87
151.82
3.74
131.09
2982.05
731.49
4998.87
450.09
3804.79
MIR-140
1.91
1.63
6.95
0.00
5.36
MIR-141
273.58
110.40
502.94
17.18
285.18
4106.24
2751.41
11185.70
1328.93
15395.12
MIR-143
658.22
327.55
1097.02
151.76
822.89
MIR-144
6.87
8.66
14.55
8.02
18.61
1110.14
386.56
1250.66
214.01
1282.89
MIR-146
60.02
14.45
106.86
9.64
71.46
MIR-147
326.07
96.59
513.02
51.41
512.53
MIR-148a-P
186.34
48.55
300.14
65.68
315.33
MIR-142-P
MIR-145-P
MIR-148b-P
880.30
387.60
1262.86
128.64
1764.13
MIR-149
2158.86
1228.15
4215.90
563.31
2698.16
MIR-150
782.01
408.29
1389.83
178.73
1074.33
MIR-151
371.93
110.58
813.58
27.91
472.92
MIR-152
168.31
256.71
340.49
272.63
507.01
MIR-153-1,2
152.19
147.65
129.60
53.05
96.22
74.29
41.38
260.06
16.33
144.73
MIR-154
MIR-155
80.38
107.04
107.64
4.32
89.60
MIR-181a,c
447.28
620.27
806.30
97.59
409.56
MIR-181b-1,2
139.44
99.19
229.24
27.85
242.17
MIR-182
7325.60
1833.42
3938.31
465.06
3876.78
MIR-183
327.81
96.59
192.64
33.02
210.72
MIR-184
1892.12
905.51
3621.02
671.69
1185.44
MIR-185
6032.23
2332.06
9623.37
1500.70
3822.44
MIR-186-P
2659.76
1744.18
9527.89
550.08
3317.72
MIR-187
181.07
202.73
517.92
197.08
256.41
MIR-188b-P
752.37
498.70
166.67
994.36
141.09
MIR-190
15.83
59.15
14.99
39.90
39.63
MIR-191
151.52
49.75
286.89
6.90
155.01
Continued
233
MIR-192
0.00
0.00
0.00
0.00
0.00
MIR-193
462.00
136.08
738.65
206.70
605.22
MIR-194-1,2
279.89
88.99
548.62
16.74
411.24
25899.02
MIR-195-P
15927.75
6364.69
33332.36
3295.25
MIR-196a-1
453.71
411.79
481.64
189.05
662.24
MIR-196a-2
327.33
46.25
803.54
130.94
282.45
MIR-196b
7154.48
2691.84
3038.97
2540.60
3251.90
MIR-197
17908.72
10930.58
19754.36
4470.89
16845.99
MIR-198
12.34
292.61
13.19
2.04
70.93
MIR-199a-1-P
3457.14
2498.51
3105.85
2122.33
4079.92
MIR-199a-2-P
696.18
3597.22
1302.08
2368.02
900.48
MIR-199b-P
5659.02
2204.29
8779.40
1289.17
5365.19
MIR-200a-P
100103.73
101638.35
144466.94
64438.67
171307.39
MIR-200b
126.85
139.39
404.22
62.70
304.12
MIR-200c
235.17
45.70
539.77
58.61
364.74
MIR-203
151.04
117.60
317.63
40.81
267.75
MIR-204
3233.23
1203.17
6361.16
694.51
6005.70
MIR-205
2749.62
1267.39
2885.66
790.15
4024.49
MIR-206
352.40
140.91
701.21
49.18
666.24
MIR-208
113.87
122.62
252.76
52.96
228.10
MIR-210
15197.87
24887.13
24766.25
23943.21
8658.47
MIR-211
6933.81
3403.82
12057.56
1726.98
8537.68
MIR-212
631.76
240.09
1060.80
153.28
883.01
MIR-213
191.89
179.01
485.61
85.86
200.71
MIR-214
3786.62
2262.78
8176.70
956.18
5818.80
MIR-215
92.32
52.54
131.80
38.23
171.35
MIR-216
601.33
256.10
1134.51
133.74
822.36
MIR-217
12.64
2.04
19.29
5.59
11.87
118.76
2951.44
207.92
1159.07
143.27
218.89
MIR-218-1,2
MIR-219-1,2
82.25
366.00
135.39
295.67
MIR-220
840.95
246.02
647.88
208.23
469.76
MIR-221
671.66
167.92
586.67
156.66
1236.55
MIR-222-P
640.72
239.75
969.30
114.25
581.48
MIR-223
254.31
39.10
290.81
17.41
314.21
MIR-224
482.70
162.84
664.38
149.62
1078.74
MIR-296
593.97
213.61
1582.79
92.71
1016.76
MIR-299-P
834.48
749.58
3344.78
398.50
2253.92
MIR-301
402.60
710.74
691.72
612.24
736.99
139.47
MIR-302
87.36
278.52
151.71
45.39
MIR-302b
384.89
174.11
482.25
68.35
425.28
MIR-302c
405.12
129.41
1129.58
24.63
777.64
Continued
234
MIR-302d
333.91
115.87
837.21
21.62
645.86
MIR-320
495.18
330.76
1074.47
152.23
1046.60
MIR-321
12633.16
7751.71
12660.01
7624.21
38764.26
MIR-323
307.22
231.00
498.40
103.57
684.46
MIR-324-P
964.56
884.51
1556.49
349.54
1590.87
MIR-325
643.14
45.07
141.98
80.62
157.49
MIR-326-P
145.30
45.63
307.32
39.55
192.77
MIR-328
6705.11
1794.17
11160.98
878.38
10478.66
MIR-330
976.01
323.99
1695.54
177.45
1643.84
MIR-331-P
1153.36
881.57
293.60
1598.51
74.19
MIR-335
38.60
14.04
86.72
3.04
35.00
MIR-337
1302.14
526.90
2495.57
368.88
2050.33
MIR-338
1383.21
474.44
1826.35
189.56
1887.33
MIR-339
1522.38
791.77
1388.57
503.36
1472.69
MIR-340
166.89
137.59
587.12
42.34
365.17
MIR-342
594.31
125.11
1781.11
133.77
1409.85
MIR-345
5520.16
1625.70
7090.30
1002.69
8379.26
MIR-346
2615.44
958.33
5360.92
516.74
2845.74
MIR-361
105.57
66.89
124.44
110.76
303.97
MIR-367
4.57
12.52
44.95
1.59
3.31
MIR-368
12.66
4.00
23.24
2.92
11.53
MIR-369
1.68
0.00
0.00
0.00
0.00
MIR-370
487.40
364.35
898.71
168.74
587.33
MIR-371
3061.09
1682.88
4857.15
622.86
3302.94
MIR-372
43.68
3.79
72.51
27.82
64.05
MIR-373
557.55
175.04
1249.87
89.80
597.21
0.00
1.03
7.89
0.00
0.00
MIR-375
30.10
32.92
28.42
67.27
58.26
MIR-376
3.78
147.74
2.97
47.57
42.88
MIR-377
120.72
52.02
127.89
56.21
330.70
MIR-378
104.49
11.12
92.28
67.28
242.08
MIR-379
9.64
124.19
3.01
144.09
21.24
MIR-380
22.92
6.37
6.80
3.99
57.08
MIR-381
13.91
1.17
58.61
2.51
78.44
MIR-382
50.42
15.40
14.58
3.62
66.50
MIR-383
69.88
11.88
44.99
28.25
155.36
MIR-384
0.00
0.00
0.00
0.00
0.00
1054.18
387.48
2299.62
32.64
1401.36
846.98
MIR-374-P
MIR-422b
MIR-423
568.32
274.84
3562.62
137.58
MIR-424
28.82
109.27
123.45
14.68
81.20
MIR-425
89.95
92.16
49.76
38.87
304.93
Continued
235
miRNA
let-7a-1*
T47D
Breast Cancer
MDA361
SKBR3
MCF7
MDA231
62.25
43.55
158.66
81.18
42.38
let-7a-2-P
688.29
1113.40
1507.16
860.86
563.29
let-7a-3*
111.26
74.66
53.56
51.86
205.56
let-7b*
175.54
113.98
62.44
75.15
176.07
let-7c*
0.00
11.87
12.70
3.22
10.72
let-7d*
273.02
283.48
422.64
319.91
210.57
let-7e*
8.64
8.14
17.71
7.94
9.09
let-7f-1*
479.87
214.50
441.00
439.57
279.89
let-7f-2*
19.25
18.62
30.97
18.75
12.32
let-7g*
84.74
80.38
152.01
39.30
103.62
let-7i*
121.17
34.10
42.70
105.70
248.82
MIR-001-1
111.86
441.72
560.64
136.05
82.99
MIR-001-2
271.90
377.84
700.50
374.80
235.78
MIR-007-1
76.16
122.37
175.51
243.58
129.69
MIR-007-2
60.77
66.59
168.69
104.48
97.31
MIR-007-3
40.55
32.19
43.46
39.24
32.80
MIR-009-1-P
2837.00
6147.47
6808.78
2913.26
3389.38
MIR-009-2-P
2474.60
3521.04
5729.07
2588.92
2095.63
MIR-009-3-P
62.92
59.04
72.35
78.77
39.65
MIR-010a,b
6.32
3.45
3.26
2.00
94.73
MIR-015a
287.29
332.38
611.60
219.33
150.22
MIR-015b
1226.15
983.08
1532.48
456.28
413.33
MIR-016-1
786.46
360.43
400.51
519.05
920.46
MIR-016-2
942.96
827.13
683.45
543.06
575.55
MIR-017-P
730.07
1136.57
1259.00
701.41
603.79
MIR-018
912.46
2437.49
1159.92
443.18
1616.64
MIR-019a-P
243.80
797.03
696.68
150.88
382.36
MIR-019b-1-P
1147.48
684.13
702.13
1380.25
1521.93
MIR-019b-2-P
3.83
4.76
7.75
2.44
5.49
MIR-020
2.82
5.46
7.34
9.83
2.22
MIR-021
4449.65
1695.60
3734.70
54580.57
23376.82
MIR-022-P
310.62
594.88
999.26
605.58
1611.30
MIR-023a
484.17
856.21
1263.72
648.93
533.03
MIR-023b-P
1156.68
1829.98
2632.32
2217.37
1062.31
MIR-024-1,2
6014.17
6789.07
4590.01
10811.48
5329.28
444.13
1221.73
707.49
418.93
356.35
MIR-025
MIR-026a-1,2
MIR-026b
MIR-027a, b
298.20
200.80
441.08
149.64
89.68
1499.59
1935.09
2380.76
1562.26
530.44
916.93
1477.60
1834.31
1120.67
940.45
Continued
236
MIR-028
56.31
57.74
72.81
38.91
60.11
108.76
310.95
317.41
118.38
909.67
11.38
77.04
103.42
11.28
27.64
MIR-030a,e
482.77
381.29
854.43
416.18
342.82
MIR-030b
465.52
900.25
1762.20
1069.91
1149.89
MIR-030c-1,2
719.85
851.89
959.04
684.72
454.47
MIR-029a,c
MIR-029b-1,2
MIR-030d
MIR-031-P
MIR-032
80.78
17.98
413.93
112.75
15.74
4357.22
5886.28
7941.88
4975.91
6153.68
144.78
63.81
360.54
223.48
97.75
MIR-033
23.94
15.67
26.07
17.58
10.55
MIR-034a
402.02
724.91
828.41
411.77
366.20
MIR-034b
87.83
61.84
114.55
74.69
77.56
MIR-034c
0.00
0.00
0.00
0.00
0.00
MIR-092-1
43.91
37.47
42.58
2.38
10.81
MIR-092-2-P
199.46
285.98
374.90
237.64
102.99
MIR-093
527.99
700.97
1078.20
404.09
397.40
MIR-095
6.57
10.17
32.50
15.51
16.14
MIR-096-P
8705.83
19556.09
16927.21
12463.90
15769.94
MIR-098
3923.73
3912.68
5167.08
4590.02
2513.38
MIR-099a
13.05
41.01
56.81
58.62
26.45
MIR-099b
433.84
718.37
644.30
880.44
562.50
34.23
61.54
31.70
9.39
4910.96
MIR-100
MIR-101-1
57.19
53.99
91.15
17.70
7.35
MIR-101-2
431.29
496.55
444.50
757.69
705.47
MIR-103-1,2
2824.43
3735.04
4344.53
4052.00
3637.89
MIR-105-1,2
182.63
248.15
494.46
177.27
51.29
MIR-106a-P
39.99
76.79
132.13
48.68
34.83
847.46
2181.43
544.63
1513.56
2151.66
MIR-107
3872.58
4127.91
2194.33
4054.20
5617.71
MIR-108
377.90
431.93
641.51
222.86
247.58
MIR-122a
23.34
48.77
109.86
83.45
70.56
MIR-124a-1,2,3
44.36
147.50
170.64
88.34
64.48
MIR-106b
MIR-125a
1029.68
1861.53
4424.16
1454.55
695.39
MIR-125b-1
20.24
42.81
35.40
10.71
299.73
MIR-125b-2
112.08
187.57
266.78
136.81
152.50
MIR-126
43.94
66.09
78.80
100.39
40.93
MIR-127
5745.33
5878.40
5840.05
5391.94
4800.48
MIR-128a
333.52
1081.04
1726.56
419.10
327.99
MIR-128b
113.44
175.13
210.29
96.97
103.98
MIR-129-2
4823.24
6984.59
7619.57
6112.24
4036.01
MIR-130a-P
4924.21
6820.42
10705.49
5561.44
4933.34
Continued
237
MIR-130b
MIR-132
MIR-133a-1,2
MIR-133b
MIR-134
MIR-135a-1,2
MIR-135b-P
68.69
175.56
286.05
44.94
7.86
10.36
82.39
42.26
39.88
17.01
671.92
674.48
1000.23
636.45
657.88
986.75
971.33
1173.69
887.52
915.55
2147.20
2854.69
3493.59
2083.45
3436.23
77.93
53.95
52.20
68.79
57.72
142.27
306.25
455.58
67.81
410.20
MIR-136
23.42
36.00
56.89
29.24
42.38
MIR-137*
20.93
32.20
31.22
54.54
19.87
MIR-138-1,2
MIR-139
48.47
48.96
66.88
27.36
24.62
1242.27
1423.97
1564.71
1115.19
810.34
MIR-140
0.00
0.83
3.33
0.99
1.02
MIR-141
61.85
175.44
135.60
109.94
82.61
MIR-142-P
834.07
1803.41
2639.43
1164.34
1240.16
MIR-143
147.35
443.79
502.10
234.25
164.74
MIR-144
6.16
9.64
8.24
65.90
6.29
371.05
MIR-145-P
147.76
589.08
408.63
353.07
MIR-146
8.43
13.66
27.70
16.89
17.70
MIR-147
99.63
150.80
332.58
88.94
95.80
MIR-148a-P
74.31
69.59
60.83
75.62
82.62
MIR-148b-P
857.16
586.37
1727.98
397.96
414.32
MIR-149
1387.37
1312.37
1989.93
1344.34
1761.82
MIR-150
503.48
478.36
548.66
382.17
477.60
MIR-151
137.06
174.13
353.87
255.64
129.77
MIR-152
48.91
73.20
106.41
137.98
171.43
MIR-153-1,2
86.26
31.71
37.01
57.91
40.75
MIR-154
4.63
30.12
39.11
11.69
64.67
MIR-155
10.48
41.57
150.38
105.92
382.40
526.25
320.93
352.45
164.21
894.54
MIR-181a,c
MIR-181b-1,2
90.07
185.35
226.58
101.45
194.93
MIR-182
4001.65
4445.20
1426.94
8261.33
8280.74
MIR-183
148.97
191.99
98.05
291.27
390.85
MIR-184
1411.99
1536.55
2368.26
1542.60
1152.12
MIR-185
2970.97
3586.65
3673.96
3417.54
2484.17
MIR-186-P
1776.40
3199.59
4034.67
2033.39
1559.13
MIR-187
58.54
146.67
173.64
125.80
61.46
131.00
171.89
358.77
169.89
108.23
MIR-190
12.21
27.22
28.14
0.95
2.63
MIR-191
43.94
69.87
92.95
46.02
12.11
MIR-192
0.00
0.00
0.00
0.00
0.00
MIR-193
347.41
306.98
320.03
203.38
206.30
MIR-188b-P
Continued
238
MIR-194-1,2
36.90
108.57
56.38
96.72
41.80
MIR-195-P
4392.29
8430.40
8891.78
5641.60
8186.25
MIR-196a-1
121.91
210.47
429.53
334.39
143.51
MIR-196a-2
377.53
316.74
617.67
162.95
249.69
MIR-196b
222.43
863.03
1063.27
445.86
735.45
7025.10
24608.73
13504.50
3916.36
1973.59
MIR-197
MIR-198
30.19
7.97
9.39
1.77
2.97
MIR-199a-1-P
2006.88
2718.44
3351.06
2476.13
1998.50
MIR-199a-2-P
331.62
989.45
1328.12
360.36
244.54
MIR-199b-P
2375.35
3000.15
3298.06
3261.80
2230.47
MIR-200a-P
57526.35
32110.60
68020.47
46917.79
66490.12
MIR-200b
93.33
58.59
234.11
174.94
34.20
MIR-200c
88.20
136.54
222.29
145.93
57.19
MIR-203
88.75
61.64
50.97
39.79
7.83
MIR-204
803.02
1462.54
2400.18
379.38
1870.90
MIR-205
273.21
77.85
234.10
28.61
22.36
MIR-206
11.87
120.80
339.45
115.18
31.33
MIR-208
61.45
94.89
84.41
61.91
39.13
MIR-210
1251.49
10642.91
8897.04
13227.19
15161.91
MIR-211
3511.02
3749.33
6996.25
3879.47
3316.68
MIR-212
124.33
206.66
323.50
259.13
437.06
MIR-213
270.56
235.05
201.22
264.04
725.56
MIR-214
1818.16
2440.67
3668.78
1916.36
1540.07
MIR-215
174.40
178.94
249.84
87.29
124.09
MIR-216
192.32
280.14
408.72
228.03
181.29
MIR-217
MIR-218-1,2
MIR-219-1,2
7.41
26.01
17.80
7.90
2.19
28.19
593.60
709.95
94.89
20.51
84.76
132.03
130.01
49.52
148.28
MIR-220
149.46
258.83
299.02
289.09
680.37
MIR-221
35.67
37.02
38.03
37.22
243.54
MIR-222-P
56.47
110.29
160.25
133.10
99.38
MIR-223
54.56
135.34
217.84
85.06
53.51
MIR-224
181.41
286.00
281.54
168.92
142.44
MIR-296
251.92
89.98
538.08
334.28
253.02
MIR-299-P
585.45
920.19
1897.78
2717.80
569.88
MIR-301
788.21
286.71
374.65
1137.02
612.53
MIR-302
89.48
94.55
277.30
120.71
23.64
MIR-302b
165.52
346.73
306.09
226.29
137.81
MIR-302c
115.90
191.66
321.50
161.90
131.02
MIR-302d
128.09
220.19
275.97
84.05
86.58
MIR-320
409.76
438.32
750.84
393.78
488.45
Continued
239
MIR-321
2457.71
7312.89
15327.99
68262.76
MIR-323
619.72
592.20
526.24
169.92
96.00
MIR-324-P
571.93
614.68
926.84
794.07
519.32
MIR-325
42.11
40.02
37.14
105.82
17.09
MIR-326-P
34.98
49.61
101.96
83.70
32.27
MIR-328
1339.08
2747.11
4416.14
1934.46
1887.74
MIR-330
272.23
342.89
674.43
464.94
357.97
MIR-331-P
201.32
583.44
987.66
406.33
365.48
MIR-335
8672.16
15.48
23.83
16.20
18.10
MIR-337
460.97
774.96
697.24
75.04
530.19
MIR-338
625.99
872.31
1090.44
702.12
634.21
MIR-339
987.15
965.65
943.34
978.02
764.80
MIR-340
602.43
212.03
956.98
864.57
1010.18
251.75
11380.68
MIR-342
252.31
317.36
403.08
450.77
MIR-345
1529.57
2797.94
3450.28
1806.99
523.98
MIR-346
1247.74
1507.08
1152.95
2164.04
1719.51
MIR-361
165.11
82.17
50.93
198.82
96.81
MIR-367
8.28
24.84
17.59
40.50
23.38
MIR-368
0.00
0.94
1.99
6.15
3.12
MIR-369
0.00
2.16
0.00
0.00
0.00
MIR-370
167.34
193.30
369.51
198.67
144.19
MIR-371
841.11
1591.51
1663.46
1318.69
1749.81
MIR-372
5.36
7.48
38.52
19.75
4.56
MIR-373
194.64
286.49
353.84
228.32
151.31
MIR-374-P
24.94
5.49
6.11
1.57
0.00
MIR-375
59.32
25.95
101.19
72.24
17.45
MIR-376
4.13
2.51
2.07
15.51
7.56
MIR-377
109.56
11.34
30.87
347.19
71.63
MIR-378
37.30
56.46
54.87
234.68
63.52
MIR-379
1.44
7.31
6.37
16.52
2.84
MIR-380
11.86
4.24
34.21
4.58
4.52
MIR-381
15.27
6.16
36.54
27.24
8.73
MIR-382
9.67
7.87
9.30
14.27
27.48
MIR-383
66.51
20.24
28.99
130.99
84.30
MIR-384
0.00
0.00
0.00
0.00
0.00
MIR-422b
658.88
831.07
1622.54
853.33
473.31
MIR-423
232.65
350.31
270.22
225.82
200.45
MIR-424
12.14
89.07
56.92
126.24
206.92
MIR-425
217.74
155.79
565.54
89.11
233.39
Colorectal Cancer
Continued
240
miRNA
HCT8
HCT116
HT29
SW620
HCT15
HeLa
let-7a-1*
858.6328
26.56572
103.2427
118.4805
183.6582
25.74712
let-7a-2-P
396.5826
1448.423
904.466
944.8584
1740.836
1857.928
let-7a-3*
779.3264
33.83821
460.5481
191.8136
388.4083
71.64201
let-7b*
666.7015
140.1044
430.6308
177.4954
416.9417
150.0497
let-7c*
2.139169
1.966706
let-7d*
1652.745
319.0011
623.4727
643.5575
287.5726
111.6563
let-7e*
17.60674
27.02524
16.84116
let-7f-1*
1983.959
480.2421
847.6834
737.6523
495.6824
132.7326
let-7f-2*
67.00685
24.49063
38.95639
21.29969
18.34627
9.176052
let-7g*
625.3398
143.5549
63.57787
256.2802
163.6351
26.97658
let-7i*
225.3582
87.03372
51.60207
180.7823
160.3876
228.2109
MIR-001-1
29.96919
125.0151
15.76709
218.114
331.3349
320.1529
MIR-001-2
452.1449
261.2442
265.1213
306.5967
1177.074
337.7787
MIR-007-1
88.78428
148.2617
158.5793
173.1882
262.1453
102.6599
MIR-007-2
26.99634
49.14339
141.9696
137.9683
146.9345
207.0472
MIR-007-3
2.197971
23.89761
59.15871
4482.319
41.89004
149.18
MIR-009-1-P
1451.901
6319.989
4207.668
2610.739
4601.714
1455.045
MIR-009-2-P
913.5585
2653.155
2516.776
3651.288
7360.267
7251.747
MIR-009-3-P
5.910899
74.97863
62.53537
60.41976
67.56843
96.99898
619.1
86.19631
266.6186
78.49641
1901.472
56.19538
MIR-015a
145.9512
378.2724
333.8203
495.4617
776.2445
502.9424
MIR-015b
386.8024
931.5294
706.1382
900.2918
1107.319
502.2059
MIR-016-1
660.0388
876.3006
702.3629
1205.397
2270.934
276.4983
MIR-016-2
300.8195
738.2241
727.1829
613.9356
858.8586
1157.982
MIR-017-P
424.6995
1359.089
1377.29
3260.714
2047.729
900.4742
MIR-018
2081.512
2616.175
6256.62
12520.61
8806.856
1728.682
MIR-019a-P
416.7196
731.731
1286.29
4039.599
3258.111
1022.288
MIR-019b-1-P
864.9197
859.0292
1785.739
1182.591
1936.753
579.6239
4.062523
MIR-010a,b
MIR-019b-2-P
4.336451
10.56738
5.713239
MIR-020
7.505857
14.61272
28.04179
32.71384
11.72935
1.32159
MIR-021
50874.99
58938.4
17778.59
14369.48
4720.269
6712.824
MIR-022-P
1909.665
712.7892
1911.439
2520.227
757.4464
733.3245
MIR-023a
240.2744
592.8306
716.3597
779.2062
1407.155
1201.024
MIR-023b-P
675.0464
1962.236
1303.544
1594.949
2894.791
360.3628
MIR-024-1,2
3769.404
3275.857
7780.918
10350.49
10848.19
6529.597
MIR-025
418.9766
438.5579
1004.11
1740.284
1624.791
506.4707
MIR-026a-1,2
246.9806
145.2754
233.6056
304.7698
298.4189
318.475
MIR-026b
672.3596
1600.749
1511.269
1402.817
2249.309
1154.567
MIR-027a, b
603.8183
1323.765
1324.007
1223.712
1941.031
1531.911
MIR-028
18.96326
31.46524
33.42726
47.57557
81.76565
42.16035
Continued
241
MIR-029a,c
175.5249
923.1972
241.7734
748.8847
967.5754
109.7668
MIR-029b-1,2
3.131238
25.64242
73.733
13.69045
26.82112
21.42734
MIR-030a,e
131.2142
186.5888
241.3156
259.7985
622.7983
360.9059
MIR-030b
293.2278
1113.478
1116.101
991.1852
1655.255
941.8097
MIR-030c-1,2
425.1509
691.1524
853.9301
679.8306
1435.298
729.6663
MIR-030d
50.05628
95.89363
172.0392
103.4067
182.5863
29.27537
MIR-031-P
3636.022
9515.312
6730.927
4758.911
11835.72
6526.764
MIR-032
175.8589
116.0979
187.4735
137.3989
555.828
131.0234
MIR-033
6.380136
7.845837
13.69873
26.96014
21.39319
32.09109
MIR-034a
114.7969
404.5851
689.6787
508.8551
616.5719
509.5557
MIR-034b
43.90841
151.7012
76.38421
114.3459
151.8128
52.63946
MIR-034c
MIR-092-1
52.24811
52.30043
95.98827
156.6745
251.6343
47.65748
MIR-092-2-P
89.67869
311.23
277.6539
203.2726
379.0785
297.8797
MIR-093
292.1088
723.8734
827.0072
939.9623
986.3213
420.677
MIR-095
47.0068
26.42547
4.353642
19.85175
51.82277
14.76487
14151.99
MIR-096-P
7814.41
23747.99
17050.31
14472.33
37251.3
MIR-098
2120.708
2253.724
3885.721
9781.361
13142.37
3682.978
MIR-099a
6.977367
25.569
20.92022
26.31396
136.5722
97.52835
MIR-099b
436.9488
243.2771
491.9417
230.9324
1442.155
1442.525
MIR-100
7.230838
8366.922
16.65482
405.3392
54.78532
1002.784
MIR-101-1
26.54289
7.940138
10.34086
61.59516
44.3979
21.81055
MIR-101-2
223.2018
361.7411
537.9242
1202.656
655.1078
247.3087
MIR-103-1,2
1947.736
3288.189
3458.059
5432.269
8465.019
3404.049
MIR-105-1,2
107.2489
193.9397
217.3292
202.5615
363.8384
343.6853
MIR-106a-P
17.82535
77.62886
71.00889
83.51111
116.1518
73.04081
1194.63
1314.696
3225.651
4127.674
3566.511
607.9477
MIR-107
2766.868
3405.163
4607.7
8226.097
12181.71
2083.073
MIR-108
94.60239
278.2872
322.534
190.7989
512.1909
3.082367
MIR-122a
7.459651
36.69896
29.73935
43.41953
111.0434
155.046
MIR-124a-1,2,3
13.92713
75.44121
63.41554
100.1347
167.9732
65.45739
MIR-125a
312.2859
1121.213
730.2028
743.4873
1311.396
756.9644
MIR-125b-1
4.426267
1165.244
32.6417
47.81384
44.05668
168.745
MIR-125b-2
29.39134
197.8012
124.2387
78.89522
83.40107
123.868
MIR-126
45.1213
131.4173
66.74584
72.37189
29.29237
428.2762
MIR-127
3789.418
3821.264
5811.132
4529.653
3397.028
4810.069
MIR-128a
409.2436
617.281
398.6055
323.1376
1078.223
540.5957
MIR-128b
24.79734
67.95487
81.44437
106.2937
81.75552
132.3815
MIR-129-2
3252.573
7358.586
6573.032
5799.161
9972.255
8423.595
MIR-130a-P
2527.761
7547.183
6471.418
6467.01
8418.279
6466.665
MIR-130b
60.07852
20.81728
45.86715
110.1567
154.7273
40.43121
MIR-106b
Continued
242
MIR-132
3.392268
10.54607
30.60755
43.08519
43.57966
39.57838
MIR-133a-1,2
593.6712
664.8818
671.4538
449.8052
1335.077
551.3551
MIR-133b
414.7147
1359.772
1029.132
1266.517
512.5088
1947.167
MIR-134
1134.351
1901.47
2974.258
2484.47
426.9678
3013.016
MIR-135a-1,2
108.5499
35.63658
59.46605
108.3684
399.6547
94.2825
MIR-135b-P
213.8267
495.0453
333.3833
292.3017
162.6791
348.2613
MIR-136
16.42341
32.59869
23.23807
35.61811
67.26193
81.64402
MIR-137*
6.706809
17.59405
22.47996
39.4592
45.94706
3.584687
MIR-138-1,2
7.840621
33.79533
20.07316
19.30726
60.12293
54.64548
MIR-139
676.9869
1964.388
1506.958
1148.339
1990.836
1455.485
MIR-140
0.99355
0.77326
2.08558
MIR-141
96.90152
75.62146
101.0013
51.94906
237.3693
153.924
MIR-142-P
742.1889
2180.873
1944.425
93.08532
2444.312
2174.696
514.8569
MIR-143
116.873
242.5868
208.6492
144.0841
514.7473
MIR-144
4.672227
0.628385
2.17791
3.061594
5.872603
MIR-145-P
161.9171
523.5928
388.4528
313.7008
351.346
243.1834
MIR-146
4.326121
182.8769
15.50638
29.38146
11.5396
31.6192
MIR-147
52.03633
190.5483
161.8781
343.2752
332.4611
200.0979
MIR-148a-P
23.38892
104.532
63.98417
51.15979
80.02053
15.87187
MIR-148b-P
191.9018
503.0921
378.48
466.9279
1703.11
682.4618
MIR-149
273.87
263.1702
1473.368
1224.909
2369.59
1814.888
MIR-150
225.8272
463.4491
518.1132
495.5266
795.23
928.579
MIR-151
48.46468
221.9175
242.1159
248.1392
277.7097
394.1336
MIR-152
77.45324
69.03609
43.70144
75.43123
196.9967
114.6715
MIR-153-1,2
13.86209
103.8692
22.36573
265.4505
91.39711
25.4118
MIR-154
3.869608
22.38215
33.1496
24.34255
38.98702
38.05686
MIR-155
12.42286
55.04169
77.95959
15.36145
259.746
134.442
MIR-181a,c
143.8996
332.8634
561.3267
1189.489
427.5878
453.3771
MIR-181b-1,2
32.94578
110.9696
69.27151
65.49388
191.7068
164.9875
MIR-182
3471.177
11760.68
7875.101
14851.87
11546.05
1052.715
MIR-183
167.8081
493.7559
211.6685
452.9504
314.8324
55.94216
MIR-184
482.8474
1217.5
1302.477
1503.389
2430.265
1696.498
MIR-185
1655.257
3543.113
3587.202
2314.415
3844.361
2252.288
MIR-186-P
1534.839
2294.337
3083.847
3085.927
9961.895
2040.209
48.8742
172.2086
94.01175
85.77991
77.46041
150.1551
208.9167
MIR-187
MIR-188b-P
85.91987
224.7902
168.7507
185.2942
242.2323
MIR-190
1.627128
1.100183
16.3053
12.03259
20.90265
MIR-191
19.70146
25.8896
32.86388
47.7036
53.94927
47.63331
MIR-192
1074.752
523.274
610.6581
519.2557
MIR-193
94.17563
198.2033
156.1941
46.82278
246.4542
334.6753
MIR-194-1,2
28.68354
77.98645
111.8687
147.7562
117.6403
54.10766
Continued
243
MIR-195-P
3503.953
13905.82
8484.665
7887.77
15530.54
7771.25
MIR-196a-1
1109.454
313.8951
1008.097
287.2314
926.9358
494.4576
MIR-196a-2
89.39692
101.5213
148.4484
200.5871
369.093
65.10065
MIR-196b
430.8136
14753.53
24194.42
11119.59
10390.38
3911.31
MIR-197
3171.504
12127.31
5059.421
5976.362
14323.88
15025.78
MIR-198
0.810397
41.22313
3.835345
3.398553
6.038527
9.890589
MIR-199a-1-P
858.5674
1301.236
2448.376
3025.907
1992.933
1805.783
MIR-199a-2-P
157.97
320.2873
260.9154
592.8856
513.4992
195.426
MIR-199b-P
1029.394
3249.48
1077.528
2604.771
4301.675
6835.134
MIR-200a-P
36539.68
92229.37
75610.08
49235.55
125174
55241.49
MIR-200b
163.5854
62.35029
760.2065
172.5957
204.7594
108.5994
MIR-200c
108.2049
35.02744
137.3241
76.14315
202.6843
239.9847
MIR-203
94.28691
14.61154
60.7142
73.73222
163.786
77.26294
MIR-204
840.4258
1995.016
1954.807
1305.777
2403.835
1853.281
MIR-205
5.302058
6.012355
21.23884
38.19757
35.29727
20.44176
MIR-206
31.23536
171.1748
226.168
184.5706
427.2351
142.4579
MIR-208
13.67356
85.60709
68.01402
66.61015
122.5556
144.5863
MIR-210
4693.817
29509.37
8259.725
6299.346
29003.47
63.51755
MIR-211
1760.765
3780.557
3280.637
3808.365
6854.794
3392.772
MIR-212
238.8805
492.1094
246.0567
881.8622
1346.46
404.9515
MIR-213
35.85119
722.4231
436.9843
360.1484
358.7738
142.3082
MIR-214
1040.015
1724.102
3260.109
1721.53
2832.766
1827.1
MIR-215
53.17391
103.3322
76.92807
59.78262
155.5646
53.13787
MIR-216
112.0725
277.7547
306.5443
259.5179
359.4469
351.6544
MIR-217
15.26014
6.470761
3.532712
2.278755
19.34929
3.729676
MIR-218-1,2
7.637745
66.02772
30.49248
39.07788
60.19512
72.05416
MIR-219-1,2
83.49827
42.5829
47.46584
88.41967
198.1093
71.7328
MIR-220
123.3407
245.8683
202.4307
179.7148
509.197
282.5261
MIR-221
107.3138
794.0723
273.5634
397.8852
343.9514
127.902
MIR-222-P
31.33221
3017.878
166.1777
120.7482
339.7038
231.9186
MIR-223
27.62982
27.98896
64.82215
64.75493
101.7956
3.497619
MIR-224
88.74434
129.1676
272.4346
250.7757
263.6765
855.595
MIR-296
95.04047
354.2562
342.4293
228.2298
361.4993
768.5301
MIR-299-P
368.4251
1967.997
682.9783
860.2205
1398.926
1444.011
MIR-301
252.6409
267.3405
234.8289
930.1332
1532.321
213.291
MIR-302
18.28203
63.12679
81.33678
56.05573
86.99968
27.34738
MIR-302b
83.838
98.12517
143.8903
169.9355
305.597
318.0692
MIR-302c
50.42963
172.1014
169.3356
135.0086
302.1697
0
183.7419
MIR-302d
39.031
163.2747
178.0246
131.7586
245.5951
MIR-320
489.3002
412.7287
502.0868
609.2567
1476.951
532.4651
MIR-321
5699.42
20846.95
16162.7
5800.888
20094.89
5317.977
Continued
244
MIR-323
65.27868
129.7469
119.1797
137.3871
411.4272
382.2681
MIR-324-P
537.6329
619.6461
792.0435
839.5042
1588.497
690.3973
MIR-325
68.18792
42.85667
70.74943
313.2638
114.5458
42.42922
MIR-326-P
6.368802
67.8049
48.52222
43.86994
83.51881
104.3394
MIR-328
1001.711
3079.63
2991.854
2181.811
4949.716
4486.09
MIR-330
268.1315
545.9928
374.8421
402.7715
576.319
511.4956
MIR-331-P
268.4213
368.9336
426.6046
389.2076
912.9941
30.81881
MIR-335
11.53739
34.5304
11.36867
4.327626
8.429286
5.887744
MIR-337
410.4399
567.2167
520.4553
646.2871
1048.427
769.6332
MIR-338
202.4377
891.3485
467.7347
693.0709
1121.582
278.0501
MIR-339
553.6613
1137.904
938.9432
678.1472
1517.897
1570.045
MIR-340
506.743
535.116
406.8421
471.7936
1320.466
231.1919
MIR-342
97.63136
323.29
242.2573
202.1271
402.1698
856.0626
MIR-345
241.1075
1984.368
2432.859
2182.408
3279.497
561.8711
MIR-346
977.9059
2267.955
1449.065
1385.61
1998.735
2077.889
MIR-361
41.62921
156.2936
110.8631
144.5701
166.8953
129.6966
MIR-367
42.87979
21.25949
31.42357
21.77565
96.02627
4.851067
MIR-368
7.475348
2.509002
9.705547
27.33484
1.082579
MIR-369
13.40863
MIR-370
49.93002
318.7627
132.4245
141.267
356.5916
364.7185
MIR-371
267.9453
3764.923
1293.84
961.8371
1768.312
20.88434
MIR-372
4.040082
7.464502
26.28481
13.19192
15.65218
MIR-373
58.48832
264.0393
186.106
260.127
391.8836
213.7537
31.19252
13.96407
115.2487
89.98624
89.87842
44.47787
90.58668
206.0609
224.8806
11.34272
MIR-374-P
MIR-375
MIR-376
16.02958
12.74379
6.915397
17.19225
16.01698
MIR-377
21.17526
179.9386
201.2492
226.4024
443.5638
394.4266
MIR-378
36.70699
139.4146
201.9541
220.9498
409.1737
112.3007
MIR-379
7.045688
3.157919
3.566075
3.495784
54.92527
MIR-380
18.06349
12.7084
43.95977
37.94854
12.48013
MIR-381
2.0587
16.60751
47.25099
34.17626
37.03083
75.91218
MIR-382
3.435885
35.04295
43.54007
83.76556
69.52062
41.85704
MIR-383
10.71362
207.8169
173.9212
119.2419
256.8142
147.2181
MIR-384
1.575494
MIR-422b
296.4645
707.789
698.0748
765.0957
1151.567
820.1639
MIR-423
92.93823
356.5244
194.9094
158.1259
301.6657
224.4276
MIR-424
16.90948
54.37745
24.08734
286.3969
34.78159
47.78524
MIR-425
1857.477
498.9913
221.6507
396.941
400.8351
23.93992
miRNA
RAMOS
Hematological Cancer
HL60
K562
JURCAT
DAUDI
293T
Continued
245
let-7a-1*
let-7a-2-P
let-7a-3*
166.6366
13.8676
17.14962
45.91204
49.82124
13.92584
3753.81
746.0296
2851.498
2891.934
3460.508
360.4258
19.68504
21.09172
9.386879
5.125911
23.46274
let-7b*
18.98209
66.10125
10.16696
13.42023
22.75995
let-7c*
68.968
1.539028
29.12717
let-7d*
1313.732
294.2404
321.4057
307.9057
540.0203
114.0851
let-7e*
10.35786
let-7f-1*
333.5827
47.52613
77.08861
49.36752
174.2227
116.2856
let-7f-2*
81.47435
19.7481
27.74237
57.10392
45.57301
6.957312
let-7g*
963.3425
131.7437
157.3901
313.1559
299.5628
37.97679
let-7i*
154.7413
63.66212
27.74033
45.91237
137.0321
41.47092
MIR-001-1
348.7775
527.14
653.3058
101.7155
519.1936
151.9754
MIR-001-2
648.7846
432.0157
674.5672
290.1393
403.8103
707.4584
MIR-007-1
353.9373
241.9987
182.987
213.5199
241.3748
34.75693
MIR-007-2
189.9284
192.6722
285.8939
284.9488
244.211
91.25417
MIR-007-3
39.40603
57.94656
58.30487
88.11923
76.38345
191.5245
MIR-009-1-P
5285.905
342.5796
6279.909
9198.968
8508.782
3656.139
MIR-009-2-P
6173.907
670.4272
7942.355
8483.259
7111.381
1877.768
MIR-009-3-P
114.6106
11.12941
139.3301
194.4032
233.4594
63.45952
MIR-010a,b
25.52333
4.363303
4.33287
13.69781
2.646115
68.55621
MIR-015a
1274.957
364.2969
480.3505
814.0665
1220.395
226.9485
MIR-015b
2248.663
401.7331
670.3534
899.7338
2042.665
929.5878
MIR-016-1
2343.225
524.5504
326.0847
1775.581
1923.831
504.4688
MIR-016-2
1023.092
1290.183
920.9948
915.8345
1449.551
801.2603
MIR-017-P
6702.649
1519.419
3921.64
3488.064
3969.541
1486.773
MIR-018
26948.86
3956.59
58445.24
9301.178
18844.29
6476.756
MIR-019a-P
11109.49
2408.492
10702.01
3249.181
6637.693
3294.422
MIR-019b-1-P
3763.131
1137.872
1176.243
1445.265
1844.2
339.4511
MIR-019b-2-P
11.29745
2.90275
12.76187
17.86829
8.503979
MIR-020
73.75672
12.36713
27.9209
18.37304
53.46666
10.94048
MIR-021
1123.287
429.1152
3577.846
762.0187
2002.423
287.9245
MIR-022-P
584.323
657.0663
716.1434
1024.528
1125.224
481.8155
MIR-023a
1146.567
875.5624
104.4223
1127.198
1842.966
421.3858
MIR-023b-P
2697.446
2557.975
2807.599
2927.525
2907.672
769.3893
MIR-024-1,2
1817.421
2596.787
2183.636
1936.612
1583.455
1706.141
MIR-025
2925.217
862.43
963.5741
1173.715
1175.759
502.8365
MIR-026a-1,2
186.3581
419.2091
485.9575
99.1406
563.6515
174.1412
MIR-026b
2473.301
2665.344
2864.806
3211.984
4327.093
429.3476
879.3945
MIR-027a, b
2284.84
1969.356
593.0105
2948.62
3674.049
MIR-028
89.40984
74.92992
21.50215
81.95749
154.7436
20.34191
MIR-029a,c
561.8704
97.14448
13.54629
351.6844
552.4925
44.04115
Continued
246
MIR-029b-1,2
62.88458
0.638325
74.57517
94.75991
249.1745
MIR-030a,e
457.7892
558.6207
592.1317
429.6142
651.5329
154.418
MIR-030b
1676.761
866.2407
1922.159
2108.047
2645.966
733.9129
MIR-030c-1,2
1519.404
363.8571
1509.466
1442.812
2416.158
583.2534
MIR-030d
107.5944
69.02615
37.48549
73.18422
172.9553
87.41096
MIR-031-P
12019.31
1205.848
10478.9
11902.38
11238.99
4207.385
MIR-032
269.4431
124.2691
277.1414
259.4543
365.3757
92.12944
MIR-033
32.96648
11.24012
33.89762
32.09194
74.98626
8.217943
MIR-034a
1500.261
1085.274
1259.33
842.2176
1495.214
286.3861
MIR-034b
173.706
189.3395
231.1241
266.7051
248.9697
82.72159
MIR-034c
MIR-092-1
253.5061
58.98973
807.4232
171.0063
141.5984
117.4945
MIR-092-2-P
400.2367
612.5352
576.7292
775.0618
744.3792
81.69878
MIR-093
1526.799
508.0304
828.0654
1383.011
1220.094
328.1417
MIR-095
9.206388
12.0491
4.199426
7.690361
16.45554
19.71498
MIR-096-P
29596.91
21364.58
18939.54
15973.93
24151.32
10816.44
MIR-098
13177.82
6898.605
5111.825
7710.336
8417.604
1710.517
MIR-099a
33.02749
4.55678
52.15852
48.61796
32.51622
15.72285
MIR-099b
4169.225
459.3873
658.2106
193.2048
196.7277
990.7318
MIR-100
76.48112
89.44438
112.3623
53.02756
93.01511
17.41665
MIR-101-1
95.65256
88.42841
99.6927
75.03961
157.409
30.03772
MIR-101-2
1616.706
623.3177
1041.445
1067.626
862.1386
107.1349
MIR-103-1,2
10752.85
4888.763
5039.741
7216.911
5533.608
2408.706
MIR-105-1,2
593.0519
528.6808
487.0151
444.5546
343.2208
181.3683
MIR-106a-P
118.4912
9.463457
231.5252
215.0468
216.9674
42.91796
MIR-106b
12293.47
989.756
3279.127
2596.978
3434.494
765.6042
MIR-107
14540.57
3397.628
3983.135
7036.947
8073.282
2306.637
MIR-108
443.2815
534.8737
729.2897
163.9282
894.7754
522.8028
MIR-122a
86.95339
111.8566
209.2704
227.3072
176.4073
61.48922
MIR-124a-1,2,3
259.0413
269.9636
40.506
273.0116
264.5819
187.4368
MIR-125a
642.1005
168.9118
755.2254
857.7192
997.5324
408.3563
MIR-125b-1
56.06253
1.072293
77.62582
86.82186
122.6815
10.18362
MIR-125b-2
32.36581
328.655
130.2673
217.4513
313.9123
127.6051
62.26342
MIR-126
31.04462
231.5804
964.5492
103.8981
197.332
38.07505
MIR-127
5503.809
11899.7
5537.267
6073.212
7367.812
3427.893
MIR-128a
1913.021
970.5096
506.0228
1211.692
1065.062
467.3048
MIR-128b
322.0318
44.22209
225.0595
741.7277
37.67092
74.71641
MIR-129-2
15514.78
8406.395
10838.85
7713.455
9565.983
3876.494
MIR-130a-P
11760.81
8379.556
10425.72
10587.92
13496.31
4096.524
MIR-130b
277.2207
200.6143
401.3942
233.8788
189.5791
89.73827
MIR-132
7.492078
12.90677
57.81666
34.34195
68.44209
30.39035
Continued
247
MIR-133a-1,2
1014.981
133.8339
535.0988
1261.684
1665.34
340.2207
MIR-133b
1856.095
307.0956
1839.104
1788.284
2072.672
980.5206
MIR-134
5118.074
79.43878
5128.883
4638.395
4291.477
1153.442
MIR-135a-1,2
68.75971
79.38836
125.3644
187.7691
163.1687
32.19183
257.806
66.29544
699.3765
728.8616
263.905
93.29344
MIR-136
51.18809
86.71628
139.9647
121.31
197.1764
39.34398
MIR-137*
8.449507
30.54883
27.82634
27.35567
41.46985
MIR-138-1,2
58.17899
159.7229
133.4808
85.87914
154.979
12.28462
MIR-139
2263.82
1804.829
2795.394
2623.898
1877.469
1068.205
MIR-140
0.930443
1.798528
2.253947
1.814974
MIR-141
113.3429
195.1747
199.0103
275.1045
229.4861
58.2686
MIR-142-P
4970.718
4914.531
3629.009
6598.056
8289.224
1643.344
MIR-143
190.3961
175.2943
698.4452
602.3399
622.3735
324.4794
MIR-144
8.099548
11.95214
117.7107
8.643246
7.571883
MIR-145-P
164.0109
674.885
827.5368
697.4703
1059.785
406.4187
MIR-146
35.93817
27.10695
29.37064
78.74723
89.44939
7.230668
MIR-147
225.4706
253.7893
323.8461
365.772
430.9656
77.8308
MIR-148a-P
123.2298
104.3512
169.3357
247.605
123.3789
45.51435
MIR-148b-P
986.9186
877.0721
368.0117
380.3213
959.9428
292.5218
MIR-149
2042.878
987.4495
2554.727
2603.416
3547.527
1330.131
MIR-150
672.3065
598.6471
1117.75
1290.867
993.7567
419.0044
MIR-151
352.4521
257.3444
625.6778
525.5154
339.3763
336.8306
MIR-152
152.3424
29.09152
47.69829
132.6514
150.5859
63.4437
MIR-153-1,2
111.7338
78.28592
89.37798
257.7483
35.23463
26.89646
MIR-154
32.07609
57.96254
65.24155
81.0974
89.90831
22.71621
MIR-155
66.21983
69.26097
44.91643
102.4712
190.5929
48.37833
MIR-181a,c
374.4215
166.7022
234.3763
522.6439
238.5709
124.0678
MIR-135b-P
MIR-181b-1,2
266.679
296.5336
183.5354
632.9201
255.8292
69.49202
MIR-182
874.1759
510.1722
6538.934
2105.695
229.1402
1060.095
MIR-183
111.1306
7.169283
281.7026
64.65016
110.2007
17.91935
MIR-184
2764.261
2816.466
2538.674
1695.443
2345.092
1888.283
MIR-185
2820.113
3900.864
5143.746
5087.112
7930.047
2022.923
MIR-186-P
8687.487
3596.165
2774.455
5004.273
5556.093
8091.426
172.457
202.4664
213.9742
375.2911
270.0591
80.67414
307.3053
96.97499
389.0439
516.829
599.2478
95.60117
MIR-190
8.40962
9.038517
7.304781
22.14138
30.37547
1.281402
MIR-191
59.72927
127.677
146.4452
83.26219
127.9571
12.38503
MIR-192
1274.346
658.2719
1142.952
1303.27
410.5423
MIR-193
297.0796
32.63499
444.3399
407.9212
518.7426
158.4782
110.122
9.022362
205.3603
234.9899
270.2057
73.97635
12890.37
8651.802
8004.317
17193.54
22818.52
7326.805
MIR-187
MIR-188b-P
MIR-194-1,2
MIR-195-P
Continued
248
MIR-196a-1
373.5948
524.8424
624.9976
29.92536
927.8567
MIR-196a-2
308.9209
377.6943
425.1981
579.8911
696.5027
209.0003
MIR-196b
1188.553
526.462
1514.445
1269.183
1990.884
4574.995
MIR-197
9561.154
6715.475
10936.72
8552.249
10430.31
839.5568
MIR-198
10.25485
27.23975
7.496891
20.50808
31.81037
3.381763
MIR-199a-1-P
3532.271
3122.76
2012.811
5307.241
5745.302
2183.591
MIR-199a-2-P
193.8401
190.3845
331.3024
14.70907
329.9027
568.1804
559.7591
MIR-199b-P
4247.27
4209.361
4464.392
6410.907
5633.432
4673.392
MIR-200a-P
114526.1
97963.03
103183.2
66872.43
44753.15
45100.52
MIR-200b
93.98931
164.9444
187.893
172.5996
136.6537
2.442379
MIR-200c
227.6598
148.6829
293.986
300.8705
472.2938
59.00694
MIR-203
70.12542
30.84829
234.1509
160.0123
121.1458
18.54053
MIR-204
2741.664
1138.05
4929.028
4018.32
4515.916
875.1448
MIR-205
120.4677
61.92127
72.27868
91.26884
159.6102
29.07442
MIR-206
360.8429
143.2401
450.0692
96.63232
492.1118
141.9149
MIR-208
156.3394
17.50686
112.0073
180.5634
299.7183
61.85746
MIR-210
15574.55
16447.28
30876.33
24397.38
30757.05
8070.606
MIR-211
5522.785
4861.41
7371.303
10298.18
8073.044
7495.651
MIR-212
972.1366
425.6314
1344.729
935.1716
1130.86
602.2588
MIR-213
335.0652
437.0121
268.0511
1623.766
559.5108
92.23252
MIR-214
3839.569
3477.23
4920.847
5002.451
6113.85
2091.734
MIR-215
58.6363
95.30843
213.3499
179.9374
13.28411
97.30162
MIR-216
440.2898
549.3449
395.9147
565.4973
764.8198
234.5174
MIR-217
13.00112
4.448138
19.72684
14.59379
27.97344
4.222659
MIR-218-1,2
56.61873
28.3997
138.224
80.14827
11.04128
176.8762
MIR-219-1,2
201.0228
52.64486
181.6867
128.6737
121.7531
101.3339
MIR-220
444.9044
126.8869
610.8679
436.9656
433.458
176.3589
MIR-221
62.66685
187.8869
88.28524
101.7863
85.09758
382.4978
MIR-222-P
185.3001
239.6429
183.5392
376.9927
348.161
464.8981
MIR-223
78.67755
330.7864
208.076
196.1029
99.57811
43.05095
MIR-224
348.7584
343.0587
582.8114
531.6224
550.543
384.9217
MIR-296
564.9293
521.3971
741.983
896.1399
964.069
521.3143
MIR-299-P
1545.776
1356.846
1304.308
1663.249
813.0071
1309.007
MIR-301
770.6185
197.7101
557.6147
889.8942
522.8079
333.8545
MIR-302
77.66245
78.27097
154.8214
134.8323
159.654
21.95135
MIR-302b
223.9796
186.7529
234.4275
393.7961
516.4862
190.8893
MIR-302c
289.5766
317.166
445.3348
163.2359
618.8807
134.7958
MIR-302d
232.6644
362.3634
308.6238
450.1622
585.1192
96.48595
MIR-320
847.312
485.0178
693.0725
728.369
1305.679
288.541
MIR-321
13324.32
4899.416
22267.29
14451.83
8919.571
5974.062
MIR-323
298.1843
204.215
88.25127
597.1822
472.5881
102.2437
Continued
249
Table C.1 continued
MIR-324-P
2759.529
720.3999
1307.927
1801.631
2393.748
532.4331
MIR-325
152.4464
132.9968
146.3717
303.4011
240.979
18.63928
MIR-326-P
58.71795
84.53467
217.3564
192.9703
167.4323
65.94607
MIR-328
3873.451
1046.446
5290.15
6757.513
7758.295
2358.274
MIR-330
819.0916
27.09692
876.5171
1144.065
487.4847
307.855
MIR-331-P
1101.107
773.4277
852.2676
922.7296
1295.376
424.9777
MIR-335
3.411301
0.704119
29.35963
36.84565
33.96236
7.469318
MIR-337
1066.353
415.411
1622.734
1283.364
1991.248
386.1977
MIR-338
1234.583
1096.385
1766.293
2114.484
2179.54
852.4476
MIR-339
1759.958
1660.233
1366.792
1414.341
1567.968
754.5532
MIR-340
37.81213
650.7559
409.9121
1623.347
125.2216
322.266
MIR-342
590.5115
230.8284
705.2253
550.0349
1126.927
369.7657
MIR-345
3885.185
2094.946
5356.345
5017.119
7266.97
1890.927
MIR-346
2337.821
3251.396
2949.508
3453.948
3900.961
1428.855
MIR-361
242.3455
137.3803
270.5726
314.1634
376.7746
109.1156
MIR-367
39.46731
12.85456
22.22441
21.31749
28.22367
2.299073
MIR-368
5.713689
1.386273
29.81746
10.63905
9.435455
6.089259
MIR-369
2.179649
MIR-370
297.309
8.653101
461.9151
408.771
493.3364
207.6563
MIR-371
1938.035
2147.472
2155.386
4056.725
3646.81
3873.5
MIR-372
6.847668
12.55147
25.82391
31.65229
23.07713
8.65932
MIR-373
398.5249
430.5096
373.6562
727.1402
798.8929
170.4009
MIR-374-P
13.53244
1.007478
97.60728
61.02578
151.5996
4.252739
MIR-375
55.88773
74.08621
1.445392
148.4851
161.4004
34.45429
MIR-376
8.111453
8.306807
103.5633
56.44974
85.15578
10.84863
MIR-377
184.5237
73.34587
400.0104
699.9629
719.1905
133.0643
MIR-378
181.8826
205.7557
383.0841
610.5676
627.5236
344.9614
MIR-379
6.712977
16.94751
8.575964
7.988344
13.52819
18.71345
MIR-380
14.51789
4.140085
39.19494
41.00681
74.84995
7.934333
MIR-381
27.94209
0.606482
61.25342
78.07433
80.59869
47.75855
MIR-382
65.98568
77.54664
89.94332
176.4227
63.18845
13.7173
MIR-383
206.4342
149.7354
350.5311
298.1358
554.0786
101.8361
MIR-384
2.908049
MIR-422b
861.6428
1483.341
1463.262
1101.987
1367.448
520.5563
MIR-423
231.5228
206.3646
412.1263
539.5416
430.4981
493.5497
MIR-424
78.38365
37.26375
82.77558
179.1021
102.518
99.51117
MIR-425
764.9245
582.1007
136.7403
247.8503
232.1649
40.42695
250
miRNA
H23
Lung Cancer
HOP62
H522
A549
H719
hsa-let-7a
899.88
1530.13
2043.04
868.86
1373.08
hsa-let-7b
682.35
5733.94
7066.97
1891.98
1257.43
hsa-let-7c
211.92
9700.35
399.42
277.45
468.20
hsa-let-7d
158.69
268.77
87.27
55.03
30.14
hsa-let-7e
157.39
120.78
198.15
118.63
100.83
hsa-let-7g
1252.68
2000.31
1310.87
485.38
343.36
hsa-let-7i
392.94
503.21
629.73
485.35
71.92
hsa-miR-001
14.43
0.00
8.22
0.00
0.00
hsa-miR-007
914.32
745.16
81.01
269.64
415.48
hsa-miR-009
2276.04
3002.72
404.75
1347.05
1958.56
hsa-miR-009*
270.66
361.76
42.31
262.34
193.83
hsa-miR-010a
76.77
24.74
1873.87
668.02
1418.74
hsa-miR-010b
9.45
42.60
128.45
4.40
127.67
hsa-miR-015a
288.22
144.60
424.57
219.49
332.83
hsa-miR-015b
8832.73
8907.93
8829.58
3857.78
4753.76
35541.03
29566.27
41832.45
18083.14
22618.47
hsa-miR-017-3p
47.54
6.75
32.37
8.17
13.75
hsa-miR-017-5p
2262.56
hsa-miR-016
1386.56
6338.38
1641.61
950.11
hsa-miR-018
302.15
288.13
335.55
151.83
437.38
hsa-miR-019a
3122.63
5467.28
4701.67
2601.78
4276.36
hsa-miR-019b
14569.79
44195.40
22689.42
11846.99
18958.50
hsa-miR-020
17268.10
24522.55
22197.05
12957.76
24890.93
hsa-miR-021
7589.28
11119.87
12348.62
10244.70
3374.18
hsa-miR-022
176.62
228.39
479.17
245.50
153.97
hsa-miR-023a
187.84
243.34
154.58
69.30
119.97
hsa-miR-023b
77.30
68.23
336.52
570.72
20.97
hsa-miR-023bN
968.30
1778.11
5367.13
10278.06
1470.27
hsa-miR-024
47088.89
62228.03
37424.15
38647.31
42904.20
hsa-miR-025
2257.41
2990.67
1597.18
869.60
1967.47
hsa-miR-026aN
5460.20
9092.17
6596.03
2586.12
5159.77
hsa-miR-026b
4254.76
7004.17
3064.66
1900.34
4309.97
hsa-miR-026bN
2965.01
3492.98
1513.02
983.71
2129.98
hsa-miR-027a
4080.64
5572.63
4895.00
2999.93
6723.94
hsa-miR-027b
hsa-miR-027bN
hsa-miR-028
235.69
259.19
816.93
3375.89
243.33
2014.61
1243.44
5104.12
18497.50
1177.00
982.45
820.74
697.78
497.96
546.20
Continued
Table C.2. Mature microRNA expression in 37 different human cancer cell lines.
All data was generated using real-time PCR and TaqMan microRNA assays (Applied
Biosystems). Data is presented as copies of microRNA per cell.
251
hsa-miR-029a
5210.51
865.32
5425.87
1654.53
hsa-miR-029b
124.48
44.42
125.25
55.31
28.95
hsa-miR-029c
338.56
63.64
366.92
167.27
119.81
hsa-miR-030a-3p
1389.46
1948.95
17239.55
5124.64
2063.07
3412.84
hsa-miR-030c
10482.69
44316.76
11935.75
6665.34
11291.55
hsa-miR-030d
2171.37
905.56
833.59
672.53
315.54
hsa-miR-030e-3p
529.48
1536.30
576.82
272.12
1276.27
hsa-miR-031
9374.28
77314.37
23959.23
26433.63
38187.87
hsa-miR-032
96.07
116.80
81.95
0.00
284.37
122.99
hsa-miR-033
0.00
0.00
0.00
0.00
hsa-miR-034a
55.35
1.73
14.43
53.27
0.00
656.10
39.47
306.20
588.23
19.31
21.88
0.00
15.61
13.61
12.18
hsa-miR-034aN
hsa-miR-034b
hsa-miR-034b
13.42
0.00
13.53
0.00
10.44
hsa-miR-092N
12724.76
31479.87
15012.67
7909.82
14757.47
hsa-miR-093
25915.10
35420.31
12044.02
8901.47
21471.88
hsa-miR-095
90.45
298.60
0.00
26.64
73.00
hsa-miR-096
16047.13
11664.91
9902.69
14110.78
15808.94
hsa-miR-098
353.87
870.82
397.23
124.56
35.27
hsa-miR-099a
336.29
11836.72
22408.06
3649.68
5546.68
hsa-miR-099b
2355.45
2203.28
2057.37
1517.41
1783.86
211.08
6707.09
22531.58
3685.07
5723.36
hsa-miR-100
hsa-miR-101
3.97
65.46
55.38
31.29
20.71
hsa-miR-103
1273.12
2325.36
1586.77
671.09
1084.05
hsa-miR-105
3.82
0.00
0.00
0.00
0.00
hsa-miR-106a
2608.37
7696.12
1878.89
2230.82
2979.23
hsa-miR-106b
4494.90
6028.76
2247.55
1680.11
3246.68
hsa-miR-107
26.34
89.90
7.93
13.19
36.93
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
hsa-miR-125a
10142.80
13307.54
15530.30
8528.33
8695.46
hsa-miR-125b
337.48
19256.93
11525.70
1808.52
10964.15
hsa-miR-126
1600.08
2028.18
864.25
951.45
1059.80
hsa-miR-126*
323.29
340.17
129.18
158.65
182.10
hsa-miR-127
27.64
1071.81
36.15
530.14
55.70
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
hsa-miR-130a
712.60
3509.15
1127.11
405.60
1384.88
2081.01
8593.69
3133.12
1002.93
3124.95
370.06
1647.11
948.81
167.24
979.26
3865.34
1802.05
1044.98
828.13
2500.11
hsa-miR-130aN
hsa-miR-130b
hsa-miR-132
Continued
252
hsa-miR-133a
0.00
0.00
0.00
0.00
6.96
hsa-miR-134
327.64
3.35
125.74
18.27
4.80
hsa-miR-135a
12.88
3.69
0.00
0.00
2.92
hsa-miR-135b
19.75
21.80
97.17
12.45
6.23
hsa-miR-138
0.00
0.00
0.00
14.09
0.00
hsa-miR-139
27.01
0.00
0.00
0.00
0.00
hsa-miR-140
816.52
899.06
1851.63
984.77
51.11
hsa-miR-141
15.74
0.00
4.82
9.18
0.00
hsa-miR-142-3p
0.00
0.00
9.63
20.60
8.15
hsa-miR-142-5p
3.22
12.62
20.14
6.30
6.05
hsa-miR-143
0.00
0.00
0.00
2.53
0.00
hsa-miR-145
4.03
9.44
10.62
1.88
4.75
hsa-miR-146
807.79
432.21
23325.90
408.86
317.25
hsa-miR-146b
326.66
674.33
670.90
115.09
180.44
0.00
0.00
0.00
0.00
0.00
hsa-miR-148a
221.24
58.35
125.54
41.11
128.70
hsa-miR-148b
345.77
318.59
120.85
145.21
179.16
hsa-miR-149
461.03
6836.16
640.35
676.19
1738.99
hsa-miR-150
17.97
133.18
0.00
17.33
16.80
hsa-miR-152
486.99
486.31
1016.58
526.32
57.73
hsa-miR-152N
512.87
432.28
776.35
396.84
87.71
hsa-miR-153
0.00
0.00
0.00
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
233.98
7.13
1930.77
28.28
3.70
hsa-miR-181a
535.97
1370.30
958.41
70.21
139.62
hsa-miR-181b
2235.91
3401.72
2640.64
479.14
939.75
hsa-miR-181c
55.71
95.80
48.88
12.60
16.99
hsa-miR-182*
36.81
82.00
3.22
21.30
29.23
hsa-miR-183
920.69
3190.40
171.59
261.55
917.14
hsa-miR-184
0.00
18.51
0.00
0.00
12.23
hsa-miR-186
3956.10
6427.62
1191.90
1347.47
3909.96
hsa-miR-147
hsa-miR-155
hsa-miR-187
3.38
0.00
0.00
0.00
0.00
hsa-miR-188
39.51
55.79
41.90
53.28
49.86
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
hsa-miR-191
9396.24
21765.42
7137.11
2799.44
5871.74
hsa-miR-192
130.97
571.15
370.89
12855.43
173.43
hsa-miR-193
327.89
93.37
470.84
162.67
222.38
hsa-miR-194
42.36
130.47
105.19
2401.70
40.74
hsa-miR-195
944.74
372.59
407.90
267.23
212.38
hsa-miR-196a
1583.10
1966.47
529.34
511.12
2004.00
Continued
253
hsa-miR-196b
0.00
0.00
0.00
0.00
0.00
1881.88
6168.33
722.61
501.80
1540.31
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
13.01
15.21
5.27
0.00
0.00
hsa-miR-199b
0.00
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
hsa-miR-200a
0.00
0.00
244.34
279.06
0.00
hsa-miR-200b
10.47
0.00
675.85
488.72
10.49
hsa-miR-200bN
hsa-miR-197
10.78
0.00
217.68
158.67
0.00
hsa-miR-200c
326.09
117.89
157.79
231.20
52.97
hsa-miR-200cN
184.14
158.07
176.52
212.95
48.32
hsa-miR-203
633.11
220.46
51.85
25.64
871.36
hsa-miR-204
0.00
2.34
110.79
0.00
8.45
hsa-miR-205
20.59
0.00
0.00
0.00
0.00
hsa-miR-206
0.00
4.41
1.80
1.94
11.55
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
273.82
1338.65
1541.72
408.86
753.17
hsa-miR-210N
469.28
2240.51
2458.81
618.79
1233.28
hsa-miR-211
0.00
0.00
0.00
0.00
0.00
hsa-miR-212
891.54
488.73
366.67
181.64
397.98
hsa-miR-213
18.53
10.16
155.29
10.76
40.12
hsa-miR-214
35.80
99.66
14.40
5.41
29.86
hsa-miR-215
6.52
7.59
14.05
10.54
0.00
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
10.48
0.00
0.00
0.00
9.27
hsa-miR-218
119.45
791.21
1349.43
119.98
293.02
hsa-miR-219
0.00
0.00
7.21
0.00
3.06
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
hsa-miR-221
1932.07
20272.96
5132.26
1986.06
6329.01
hsa-miR-222
10244.92
89173.26
30145.83
14584.82
45016.44
hsa-miR-223
0.00
0.00
9.79
8.91
0.00
hsa-miR-296
1335.45
68.73
237.21
9.27
97.74
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
hsa-miR-301
1465.22
6640.75
1367.05
534.53
2645.06
32.86
7.04
0.00
0.00
0.00
hsa-miR-302a
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
146.12
37.48
3.70
27.90
55.39
hsa-miR-302c
109.03
35.01
0.00
11.03
33.94
0.00
hsa-miR-302c*
hsa-miR-302d
hsa-miR-320
0.00
0.00
0.00
0.00
101.39
11.14
0.00
0.00
6.91
2371.90
5266.80
1440.08
1816.07
4299.30
Continued
254
hsa-miR-321
8269.38
28905.04
7037.49
5219.78
hsa-miR-323
146.40
0.00
10.41
0.00
0.00
hsa-miR-324-3p
1101.42
2053.62
541.66
594.70
3650.79
hsa-miR-324-5p
3754.22
7474.25
950.17
1577.04
362.96
409.64
hsa-miR-325
0.00
0.00
0.00
0.00
0.00
hsa-miR-326
5.21
13.06
0.00
0.00
5.96
hsa-miR-328
1094.99
1435.59
862.58
160.85
356.59
hsa-miR-330
56.31
105.03
74.92
26.16
50.67
hsa-miR-331
3093.78
5102.36
1603.08
2702.55
3416.70
hsa-miR-335
186.40
22.60
329.18
197.52
32.70
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
22.00
13.05
14.43
0.00
33.71
hsa-miR-339
103.89
117.04
67.79
29.25
119.46
hsa-miR-340
155.56
570.79
61.89
138.57
397.98
hsa-miR-342
1785.92
9449.89
1171.69
881.74
2982.02
hsa-miR-345
1082.62
7455.65
1160.13
311.01
1967.32
hsa-miR-346
111.96
32.32
10.51
31.25
43.36
hsa-miR-361
347.33
803.89
343.55
147.04
1029.79
hsa-miR-367
12.17
8.22
0.00
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
587.51
142.34
351.36
126.68
137.22
hsa-miR-371
0.00
0.00
0.00
0.00
0.00
hsa-miR-372
163.46
200.08
124.68
149.12
234.75
hsa-miR-373
0.00
0.00
0.00
4.29
0.00
hsa-miR-374
3308.46
5076.11
2478.66
3497.09
3059.69
hsa-miR-375
331.62
597.33
17.06
146.22
318.68
hsa-miR-376a
548.37
26.52
159.70
15.51
4.18
0.00
0.00
0.00
0.00
0.00
hsa-miR-378
96.36
203.38
13.50
32.00
29.07
hsa-miR-379
423.49
15.97
83.52
21.45
3.70
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
7.66
0.00
0.00
0.00
0.00
hsa-miR-381
9.58
0.00
0.00
0.00
0.00
hsa-miR-382
678.89
25.90
200.50
31.66
23.58
hsa-miR-383
0.00
0.00
0.00
0.00
1.64
hsa-miR-384
0.00
0.00
0.00
0.00
0.00
53.43
162.05
7.39
44.75
35.60
hsa-miR-377
hsa-miR-422b
hsa-miR-423
643.72
518.82
557.33
497.64
538.65
hsa-miR-425
359.13
1755.68
271.38
81.56
361.66
0.00
0.00
0.00
0.00
0.00
let-7f(mmu)
Continued
255
miR-129-5p(mmu)
0.00
0.00
0.00
0.00
0.00
miR-137(mmu)
25.77
1012.22
158.65
77.00
251.79
miR-224(mmu)
17.03
0.00
177.18
196.26
0.00
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
miRNA
PANC-1
Pancreas Cancer
MIAPACA-2
HS766T
MPANC-96
PL45
hsa-let-7a
1182.55
1683.50
835.05
1199.63
947.23
hsa-let-7b
2018.23
2560.34
2687.39
3449.97
3574.74
hsa-let-7c
61.33
147.09
94.19
157.31
133.46
hsa-let-7d
7.42
107.35
127.44
116.36
60.07
hsa-let-7e
33.79
61.76
38.89
44.69
65.62
hsa-let-7g
575.71
1589.73
606.07
1480.28
433.40
hsa-let-7i
161.88
675.05
539.08
766.99
87.46
hsa-miR-001
292.52
0.00
0.00
0.00
0.00
hsa-miR-007
108.56
1385.86
316.94
302.28
354.06
hsa-miR-009
1019.56
0.00
47.10
30.47
283.82
hsa-miR-009*
89.09
3.87
11.47
15.05
119.81
hsa-miR-010a
4240.69
475.29
720.21
4866.12
973.01
hsa-miR-010b
284.96
48.55
3.20
212.17
10.76
hsa-miR-015a
186.19
454.62
266.51
239.80
162.86
hsa-miR-015b
7882.98
6493.87
7885.11
4970.63
7631.24
hsa-miR-016
4756.25
5654.60
4773.09
5100.24
5445.30
hsa-miR-017-3p
11.83
35.42
19.03
50.96
13.73
hsa-miR-017-5p
1049.42
1889.16
1021.35
951.88
550.43
hsa-miR-018
89.39
498.54
170.61
107.24
35.40
hsa-miR-019a
1337.52
8647.92
3837.72
2913.71
1273.45
hsa-miR-019b
7793.51
31589.36
12470.07
15457.90
7010.03
hsa-miR-020
10079.63
36529.78
14265.01
17326.28
7772.59
hsa-miR-021
3874.18
12073.29
3337.03
6557.24
3111.97
hsa-miR-022
99.85
94.40
111.69
61.83
32.83
114.99
hsa-miR-023a
65.18
78.86
82.39
75.64
hsa-miR-023b
118.28
80.38
42.16
425.54
32.55
2953.85
735.94
380.00
6726.38
808.58
hsa-miR-024
21153.36
17003.19
21389.59
31790.52
15809.04
hsa-miR-025
974.48
2118.61
1012.44
1279.18
902.75
hsa-miR-026aN
2863.57
4158.32
1293.18
7484.30
2664.44
hsa-miR-026b
1759.62
2873.32
1183.59
4609.94
1817.11
784.68
1403.41
240.49
2147.10
0.00
hsa-miR-027a
2026.78
2420.29
3194.50
2089.69
2018.47
hsa-miR-027b
286.88
133.38
51.72
1003.37
99.24
hsa-miR-023bN
hsa-miR-026bN
Continued
256
hsa-miR-027bN
2623.94
2748.54
460.93
8239.67
817.18
hsa-miR-028
665.30
765.12
409.52
684.17
413.53
hsa-miR-029a
2512.60
11118.61
3791.49
4390.23
3388.84
hsa-miR-029b
42.19
505.17
161.04
68.10
51.57
hsa-miR-029c
158.34
882.52
196.22
313.04
216.05
hsa-miR-030a-3p
2984.91
26.16
2582.91
1402.86
2014.20
hsa-miR-030c
8502.08
2950.37
4129.02
6620.50
5378.40
hsa-miR-030d
699.28
1127.14
181.31
1287.66
605.23
hsa-miR-030e-3p
474.62
213.62
188.63
283.53
141.34
hsa-miR-031
20261.73
27292.91
10.07
19862.04
36109.44
hsa-miR-032
68.16
265.80
171.55
57.39
45.96
hsa-miR-033
1.91
0.00
14.67
0.00
4.98
hsa-miR-034a
17.16
3.21
0.00
1.43
0.00
hsa-miR-034aN
67.52
148.35
0.00
0.00
19.57
hsa-miR-034b
14.26
0.00
13.02
0.00
0.00
hsa-miR-034b
2.31
0.00
10.89
0.00
0.00
hsa-miR-092N
9037.87
16595.75
8363.07
11399.15
6042.37
hsa-miR-093
9014.69
22378.67
12604.49
10082.93
7896.46
hsa-miR-095
12.14
46.01
32.00
38.50
43.70
hsa-miR-096
9105.72
17587.77
5416.77
6678.06
10488.34
hsa-miR-098
117.64
353.18
146.97
117.58
151.38
hsa-miR-099a
7302.87
6044.46
5.00
1725.94
1334.53
hsa-miR-099b
552.64
804.48
490.45
407.92
520.17
hsa-miR-100
9246.94
5731.50
0.00
1797.79
1447.18
hsa-miR-101
3.83
57.34
14.75
11.03
12.21
hsa-miR-103
674.71
1540.66
592.86
702.67
389.51
hsa-miR-105
0.00
0.00
0.00
0.00
0.00
hsa-miR-106a
1537.85
3331.72
698.90
1249.47
968.86
hsa-miR-106b
1218.82
4992.36
2116.40
1373.32
780.97
hsa-miR-107
0.00
2.68
2.95
2.56
0.00
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
hsa-miR-125a
3963.27
5238.75
2715.63
3949.27
4428.31
hsa-miR-125b
3415.95
1718.28
0.00
1174.38
1014.06
hsa-miR-126
1132.37
5267.51
1653.84
1576.51
350.67
hsa-miR-126*
155.59
863.15
219.04
217.85
61.64
hsa-miR-127
2672.65
0.00
0.00
0.00
0.00
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
hsa-miR-130a
336.84
13.02
412.84
4.32
133.14
hsa-miR-130aN
788.39
0.00
1115.50
9.43
274.30
Continued
257
hsa-miR-130b
32.89
107.90
9.01
32.33
3.69
hsa-miR-132
734.05
633.57
482.70
310.17
148.36
hsa-miR-133a
70.10
3.36
0.00
0.00
6.33
hsa-miR-134
450.08
4.06
0.00
0.00
0.00
hsa-miR-135a
13.35
15.45
25.52
29.95
20.90
hsa-miR-135b
114.52
399.82
551.19
683.13
740.04
hsa-miR-138
0.00
6.34
0.00
0.00
0.00
hsa-miR-139
0.00
0.00
0.00
0.00
2.81
hsa-miR-140
574.24
2213.56
1515.36
843.81
186.15
hsa-miR-141
7.19
12.43
3.56
166.63
436.46
hsa-miR-142-3p
0.00
7.74
0.00
0.00
0.00
hsa-miR-142-5p
6.10
5.83
3.52
12.03
3.01
hsa-miR-143
0.00
0.00
0.00
0.00
0.00
hsa-miR-145
1.48
7.73
0.00
9.18
11.31
hsa-miR-146
2767.86
440.78
329.94
1736.16
358.23
307.98
89.65
31.06
90.41
143.28
hsa-miR-146b
hsa-miR-147
0.00
0.00
0.00
0.00
0.00
hsa-miR-148a
10.84
321.30
26.82
0.00
23.25
hsa-miR-148b
149.41
203.48
82.41
124.85
42.80
hsa-miR-149
640.38
127.63
582.41
532.51
325.17
hsa-miR-150
7.26
15.00
14.54
573.32
15.54
hsa-miR-152
420.73
859.88
312.81
278.44
165.61
hsa-miR-152N
386.15
820.70
260.12
151.55
91.24
hsa-miR-153
0.00
0.00
0.00
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
hsa-miR-155
696.79
2925.00
15.02
441.07
305.47
hsa-miR-181a
136.36
23.81
0.00
692.14
81.81
hsa-miR-181b
976.10
364.42
144.23
2863.26
568.84
hsa-miR-181c
17.24
57.14
3.94
45.83
12.96
hsa-miR-182*
9.89
33.83
7.04
4.05
11.08
335.73
774.19
548.89
333.20
205.91
hsa-miR-183
hsa-miR-184
21.24
0.00
0.00
0.00
0.00
hsa-miR-186
1818.11
2979.35
2915.06
1770.43
845.94
hsa-miR-187
185.47
0.00
15.43
0.00
0.00
hsa-miR-188
24.66
32.51
21.27
42.82
22.66
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
hsa-miR-191
9238.01
6156.52
3770.53
4960.73
1719.16
hsa-miR-192
146.83
390.13
260.08
20261.48
99.27
hsa-miR-193
274.19
64.76
178.42
73.95
75.03
hsa-miR-194
76.88
107.24
58.69
5518.49
40.59
Continued
258
hsa-miR-195
356.35
240.35
235.86
260.83
226.77
hsa-miR-196a
2765.94
613.40
33.76
2339.81
17.20
hsa-miR-196b
0.00
20.83
0.00
192.75
0.00
503.16
hsa-miR-197
1125.13
793.34
1129.59
686.90
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
0.00
0.00
0.00
0.00
4.77
hsa-miR-199b
0.00
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
hsa-miR-200a
9.16
2399.83
0.00
1370.77
644.17
hsa-miR-200b
20.89
2723.08
11.64
3772.93
1961.98
hsa-miR-200bN
10.02
1633.16
4.31
1707.55
891.62
hsa-miR-200c
50.45
215.69
45.16
6084.19
16196.05
hsa-miR-200cN
36.64
18.28
14.49
5835.24
16427.93
hsa-miR-203
13.20
2956.58
0.00
22.04
578.34
hsa-miR-204
0.00
0.00
0.00
5.58
0.00
hsa-miR-205
0.00
0.00
0.00
0.00
319.45
hsa-miR-206
5.57
4.04
11.87
0.00
0.00
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
261.56
139.95
149.59
538.87
115.30
hsa-miR-210N
276.29
558.89
361.26
237.42
1120.24
hsa-miR-211
0.00
0.00
2.38
0.00
0.00
hsa-miR-212
201.69
173.23
115.82
108.38
109.83
hsa-miR-213
43.03
14.81
0.00
152.26
14.35
hsa-miR-214
12.43
2.87
0.00
9.31
15.35
hsa-miR-215
19.19
8.99
0.00
76.54
0.00
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
0.00
0.00
1.39
0.00
6.57
hsa-miR-218
55.10
1.78
10.38
0.00
12.71
hsa-miR-219
0.00
0.00
0.00
0.00
0.00
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
hsa-miR-221
2305.77
6273.71
6879.89
2067.47
3075.95
hsa-miR-222
13809.56
41793.55
36747.91
21292.04
19678.62
hsa-miR-223
6.92
1.71
8.75
16.70
0.00
hsa-miR-296
0.00
9.19
12.90
4.63
4.21
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
hsa-miR-301
395.72
1346.47
726.16
336.56
189.55
hsa-miR-302a
0.00
0.00
0.00
0.00
0.00
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
15.99
11.53
8.41
4.01
20.14
hsa-miR-302c
0.00
0.00
0.00
20.14
0.00
hsa-miR-302c*
0.00
0.00
0.00
0.00
0.00
Continued
259
hsa-miR-302d
0.00
0.00
0.00
0.00
0.00
hsa-miR-320
874.67
1589.50
3647.10
3405.09
727.09
hsa-miR-321
10347.40
6568.02
5043.85
8845.34
4634.01
hsa-miR-323
42.17
0.00
0.00
0.00
0.00
hsa-miR-324-3p
791.67
1690.38
803.86
584.20
205.87
hsa-miR-324-5p
291.56
1418.81
423.34
243.12
47.40
hsa-miR-325
2.34
3.52
1.68
2.27
3.16
hsa-miR-326
0.00
11.19
2.67
5.11
0.00
hsa-miR-328
110.91
306.86
216.12
262.31
78.76
hsa-miR-330
13.27
36.31
24.07
15.19
5.90
hsa-miR-331
2383.78
1651.38
2121.01
2371.51
1707.61
hsa-miR-335
210.79
156.04
0.00
45.66
102.41
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
0.00
0.00
15.23
0.00
13.62
hsa-miR-339
38.70
44.73
66.02
52.40
45.39
hsa-miR-340
127.84
159.50
119.79
89.82
58.09
hsa-miR-342
1470.67
10.95
0.00
17.08
0.00
hsa-miR-345
298.80
1182.03
700.93
217.21
35.67
hsa-miR-346
7.77
14.06
0.00
10.51
7.68
hsa-miR-361
228.49
285.02
165.98
377.80
169.85
hsa-miR-367
0.00
0.00
0.00
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
657.56
27.29
58.76
74.70
38.11
hsa-miR-371
0.00
0.00
0.00
0.00
0.00
hsa-miR-372
0.00
0.00
0.00
0.00
0.00
hsa-miR-373
0.00
0.00
0.00
0.00
0.00
hsa-miR-374
1435.58
2418.16
1653.68
2606.33
521.62
hsa-miR-375
13.71
13.40
38.35
19.96
407.26
hsa-miR-376a
0.00
621.81
0.00
0.00
7.40
hsa-miR-377
5.50
0.00
0.00
0.00
0.00
hsa-miR-378
90.77
65.51
112.36
58.01
23.71
hsa-miR-379
0.00
119.11
0.00
0.00
10.57
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
19.04
0.00
0.00
0.00
0.00
hsa-miR-381
0.00
0.00
0.00
0.00
0.00
hsa-miR-382
900.25
0.00
0.00
5.69
0.00
hsa-miR-383
0.00
0.00
0.00
0.00
3.49
hsa-miR-384
hsa-miR-422b
hsa-miR-423
0.00
0.00
0.00
0.00
0.00
78.19
19.30
34.96
28.43
19.65
594.46
384.58
411.21
332.21
316.29
Continued
260
hsa-miR-425
391.56
110.37
132.61
93.47
32.05
let-7f(mmu)
0.00
0.00
0.00
0.00
0.00
miR-129-5p(mmu)
0.00
0.00
0.00
0.00
0.00
miR-137(mmu)
12.71
0.00
123.57
0.00
0.00
miR-224(mmu)
0.00
0.00
0.00
221.65
0.00
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
miRNA
PC3
Prostate Cancer
DU145
PPC1
TSUPR1
LNCAP
hsa-let-7a
248.64
2305.57
1209.72
1457.35
1891.86
hsa-let-7b
1790.37
4713.23
2376.29
5686.75
21296.95
hsa-let-7c
94.51
445.03
148.22
463.82
16362.12
hsa-let-7d
16.90
98.60
153.70
90.55
146.32
hsa-let-7e
22.58
111.35
32.20
69.12
94.09
hsa-let-7g
155.05
1354.98
1139.74
1011.21
1654.15
hsa-let-7i
198.57
90.89
751.05
502.07
445.62
hsa-miR-001
0.00
0.00
1.68
0.00
0.00
hsa-miR-007
25.23
134.49
507.95
519.90
217.20
hsa-miR-009
324.61
3542.37
760.84
11.93
986.36
hsa-miR-009*
23.69
492.44
115.89
0.00
68.69
hsa-miR-010a
196.65
193.28
1028.68
1526.50
5.93
hsa-miR-010b
0.00
20.39
9.09
161.49
0.00
hsa-miR-015a
36.80
378.10
472.76
414.05
676.86
hsa-miR-015b
hsa-miR-016
998.70
5672.10
6980.34
6572.05
6602.90
1317.36
21365.37
36158.78
27371.26
34778.76
hsa-miR-017-3p
0.00
25.38
27.58
5.78
24.61
hsa-miR-017-5p
105.16
2100.65
1317.66
1852.93
2777.61
hsa-miR-018
26.84
537.88
216.08
235.85
426.26
hsa-miR-019a
169.23
6135.63
2103.06
5012.79
7732.10
hsa-miR-019b
912.67
22847.43
11275.52
23341.12
31302.75
hsa-miR-020
799.78
28984.67
14464.54
25677.52
34089.62
hsa-miR-021
161.84
3557.98
7101.08
8197.32
943.58
hsa-miR-022
8.52
152.68
406.41
175.24
66.77
hsa-miR-023a
26.39
73.95
226.08
101.91
0.00
hsa-miR-023b
18.24
113.72
191.94
21.99
41.05
156.25
3098.45
5211.46
1296.45
1011.47
hsa-miR-024
5332.92
27574.56
31197.25
31981.14
3474.57
hsa-miR-025
190.56
1603.58
1303.46
891.75
1825.62
hsa-miR-026aN
671.00
1321.81
3812.02
4712.45
6365.70
hsa-miR-026b
113.08
1578.39
1678.96
4053.37
4864.09
64.73
849.93
873.11
1880.25
2076.05
hsa-miR-023bN
hsa-miR-026bN
Continued
261
hsa-miR-027a
399.50
3240.49
5464.23
5730.23
237.64
hsa-miR-027b
0.00
500.80
1143.60
213.29
227.69
126.21
3143.08
5999.86
1103.12
1262.66
hsa-miR-027bN
hsa-miR-028
77.08
332.04
893.73
532.26
1459.06
hsa-miR-029a
437.78
1852.21
5349.11
3941.16
424.46
hsa-miR-029b
4.24
80.52
138.22
204.44
37.68
hsa-miR-029c
41.00
180.49
506.00
148.86
485.00
hsa-miR-030a-3p
22.32
40.17
1321.05
3703.89
244.84
hsa-miR-030c
1066.79
2909.14
5676.08
9925.13
5328.64
hsa-miR-030d
229.69
419.32
423.15
195.20
720.92
26.96
242.41
471.20
772.32
379.05
hsa-miR-031
4701.60
11519.16
30487.37
10080.98
9.58
hsa-miR-032
8.44
179.14
246.44
209.02
316.04
hsa-miR-033
0.00
0.00
0.00
0.00
0.00
hsa-miR-034a
9.53
0.00
71.82
0.00
92.36
hsa-miR-034aN
0.00
4.21
922.96
0.00
1087.61
hsa-miR-034b
0.00
0.00
61.73
0.00
0.00
hsa-miR-034b
0.00
0.00
13.27
0.00
0.00
hsa-miR-092N
1517.93
15305.20
8479.11
12791.92
14952.96
hsa-miR-093
981.55
19938.32
15453.07
7735.01
16114.06
hsa-miR-095
9.49
20.60
32.27
0.00
17.68
hsa-miR-096
6904.79
14288.44
19008.81
9715.03
9993.99
hsa-miR-030e-3p
hsa-miR-098
22.71
926.21
204.82
225.49
272.99
hsa-miR-099a
10434.41
26141.84
5529.25
11159.14
15743.31
hsa-miR-099b
1096.16
2115.44
574.02
1002.27
1342.27
hsa-miR-100
9604.95
26646.09
5104.97
12940.54
9477.41
hsa-miR-101
0.00
29.89
27.43
9.52
76.96
hsa-miR-103
188.42
870.50
802.03
880.06
899.45
hsa-miR-105
0.00
18.02
0.00
6.98
27.11
hsa-miR-106a
490.08
2343.29
2995.59
1939.78
2215.90
hsa-miR-106b
164.25
4335.05
2270.95
1317.71
2474.42
hsa-miR-107
0.00
28.06
10.77
9.86
32.99
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
hsa-miR-125a
5550.89
7722.41
3669.96
7011.86
8007.42
hsa-miR-125b
7241.52
9389.55
2272.91
4976.48
18641.08
hsa-miR-126
26.56
480.65
1395.29
220.48
578.73
hsa-miR-126*
26.10
131.47
217.06
53.74
160.96
hsa-miR-127
0.00
0.00
0.00
0.00
0.00
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
Continued
262
hsa-miR-130a
59.70
8.34
1008.53
1167.46
4.45
253.02
6.22
2361.61
2703.81
12.32
hsa-miR-130b
67.57
815.31
381.89
597.61
562.61
hsa-miR-132
83.47
155.89
410.04
762.79
723.04
hsa-miR-133a
0.00
0.00
0.00
0.00
0.00
hsa-miR-134
4.60
10.14
3.41
14.61
2.92
6.69
hsa-miR-130aN
hsa-miR-135a
0.00
0.00
18.29
0.00
hsa-miR-135b
23.63
13.11
520.24
61.67
0.00
hsa-miR-138
0.00
0.00
29.81
3.30
2.48
hsa-miR-139
13.58
0.00
3.52
0.00
0.00
hsa-miR-140
81.66
911.94
1572.64
1658.06
736.25
hsa-miR-141
0.00
0.00
112.29
0.00
1962.62
hsa-miR-142-3p
0.00
12.70
0.00
10.83
8.02
hsa-miR-142-5p
2.04
7.72
8.71
6.95
6.74
hsa-miR-143
0.00
0.00
0.00
2.11
0.00
hsa-miR-145
0.00
21.47
12.63
4.77
0.00
hsa-miR-146
458.52
372.96
455.69
501.98
404.83
17.87
74.41
85.26
115.10
739.34
0.00
0.00
0.00
0.00
0.00
3034.66
hsa-miR-146b
hsa-miR-147
hsa-miR-148a
3.15
1.80
221.97
25.91
hsa-miR-148b
12.81
236.38
216.50
162.04
342.35
101.46
1453.42
917.41
472.04
584.67
hsa-miR-150
0.00
4.18
0.00
0.00
0.00
hsa-miR-152
11.31
0.00
552.58
60.27
470.38
hsa-miR-152N
hsa-miR-149
78.53
57.93
483.12
81.79
462.79
hsa-miR-153
0.00
0.00
0.00
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
hsa-miR-155
8.69
11.37
488.95
6.32
8.54
hsa-miR-181a
27.55
151.57
395.55
179.42
51.82
hsa-miR-181b
230.36
909.47
1532.69
1310.84
283.96
hsa-miR-181c
8.87
13.64
44.56
14.99
2.19
hsa-miR-182*
7.32
4.58
0.00
15.14
15.03
hsa-miR-183
198.15
621.03
403.22
124.21
423.05
hsa-miR-184
0.00
0.00
0.00
0.00
0.00
hsa-miR-186
517.48
3977.23
2416.84
3818.16
5837.81
hsa-miR-187
0.00
0.00
21.14
0.00
0.00
hsa-miR-188
38.13
39.83
33.14
56.44
46.24
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
hsa-miR-191
2949.10
4882.11
5126.32
2695.08
9832.45
hsa-miR-192
10.49
257.61
488.52
215.71
405.10
Continued
263
hsa-miR-193
24.14
355.44
406.64
105.76
hsa-miR-194
14.08
47.68
120.34
67.56
99.91
hsa-miR-195
24.07
261.41
506.97
243.77
2652.71
hsa-miR-196a
7.25
306.59
929.18
802.49
142.21
hsa-miR-196b
0.00
132.30
11.28
42.61
0.00
827.04
1471.11
796.72
1748.38
2122.89
hsa-miR-197
196.37
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
0.00
10.46
18.54
0.00
12.96
hsa-miR-199b
0.00
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
hsa-miR-200a
438.34
0.00
1319.82
414.58
3629.46
hsa-miR-200b
450.01
8.24
4014.36
1179.32
9999.19
hsa-miR-200bN
146.94
0.00
1251.11
327.32
3604.62
hsa-miR-200c
35.16
74.30
3901.39
76.17
41166.56
hsa-miR-200cN
0.00
65.74
4283.23
11.04
35677.28
hsa-miR-203
0.00
0.00
30.68
0.00
1964.66
hsa-miR-204
0.00
0.00
0.00
0.00
0.00
hsa-miR-205
14.31
0.00
0.00
0.00
0.00
hsa-miR-206
0.00
0.00
0.00
0.00
3.63
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
245.92
207.03
921.80
1025.47
2171.44
hsa-miR-210N
454.75
346.15
1745.85
1753.32
3648.52
hsa-miR-211
2.16
0.00
0.00
0.00
0.00
hsa-miR-212
125.78
145.08
255.78
148.78
154.44
hsa-miR-213
0.00
25.90
45.98
23.77
0.00
hsa-miR-214
0.00
18.69
10.07
3.59
4.58
hsa-miR-215
0.00
10.08
2.52
0.00
8.82
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
0.00
0.00
0.00
0.00
0.00
hsa-miR-218
163.87
386.42
24.05
152.93
0.00
hsa-miR-219
0.00
0.00
0.00
0.00
0.00
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
hsa-miR-221
680.25
4111.80
2903.41
4537.31
509.99
hsa-miR-222
8097.75
21467.41
21031.43
27649.99
3340.37
hsa-miR-223
0.00
0.00
0.00
20.30
38.70
hsa-miR-296
2.91
5.01
280.08
18.00
63.51
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
hsa-miR-301
221.87
2116.43
783.40
861.80
1906.77
hsa-miR-302a
0.00
0.00
0.00
0.00
0.00
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
4.78
0.00
15.04
22.93
27.64
Continued
264
hsa-miR-302c
0.00
0.00
0.00
0.00
0.00
hsa-miR-302c*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302d
0.00
0.00
0.00
0.00
0.00
hsa-miR-320
1053.72
2470.85
1721.10
3163.14
2100.71
hsa-miR-321
1495.88
6988.05
7266.64
8810.17
7492.00
hsa-miR-323
0.00
0.00
0.00
0.00
0.00
hsa-miR-324-3p
144.75
696.30
196.62
2110.81
1876.14
hsa-miR-324-5p
153.72
860.59
489.33
1144.39
1267.45
0.00
0.00
0.00
0.00
0.00
hsa-miR-325
hsa-miR-326
0.00
11.37
0.00
1.60
15.09
hsa-miR-328
415.86
129.24
218.75
127.19
538.61
hsa-miR-330
15.40
91.85
13.01
32.01
26.88
hsa-miR-331
1533.35
2960.79
4388.04
2221.64
2578.03
hsa-miR-335
12.29
0.00
266.29
0.00
152.22
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
0.00
0.00
0.00
0.00
0.00
hsa-miR-339
29.65
55.60
90.30
46.57
86.36
hsa-miR-340
19.51
103.84
177.12
91.73
201.86
hsa-miR-342
444.31
3312.30
2566.61
908.06
1626.29
hsa-miR-345
283.42
1004.11
593.95
348.95
940.61
hsa-miR-346
11.62
24.95
16.93
22.04
17.16
hsa-miR-361
29.22
527.75
236.79
264.04
270.05
hsa-miR-367
0.00
0.00
1.55
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
109.86
133.32
82.30
69.82
96.83
hsa-miR-371
0.00
0.00
0.00
0.00
0.00
hsa-miR-372
67.28
124.89
91.78
211.41
100.90
hsa-miR-373
0.00
0.00
0.00
4.43
13.40
hsa-miR-374
62.62
2869.91
1750.01
2124.95
2628.65
hsa-miR-375
32.16
38.94
141.10
0.00
417.78
hsa-miR-376a
10.80
11.67
0.00
20.40
0.00
hsa-miR-377
0.00
0.00
0.00
0.00
6.41
hsa-miR-378
0.00
58.24
94.01
41.80
80.50
hsa-miR-379
2.53
21.94
0.00
11.40
10.02
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
0.00
0.00
0.00
14.79
27.08
hsa-miR-381
0.00
0.00
0.00
0.00
0.00
hsa-miR-382
0.00
26.09
0.00
28.16
0.00
hsa-miR-383
0.00
2.29
0.00
0.00
3.45
hsa-miR-384
0.00
0.00
0.00
0.00
0.00
Continued
265
hsa-miR-422b
2.35
90.48
27.68
61.72
37.44
hsa-miR-423
70.66
335.16
647.27
439.82
384.63
hsa-miR-425
88.57
423.28
156.14
184.42
428.14
let-7f(mmu)
0.00
0.00
0.00
0.00
0.00
miR-129-5p(mmu)
0.00
0.00
0.00
0.00
0.00
miR-137(mmu)
0.00
0.00
23.25
649.01
0.00
miR-224(mmu)
0.00
0.00
0.00
0.00
18.08
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
miRNA
SCC17A
Head & Neck squamous cell carcinoma

SCCD12
SCC10B
SCC17B
SCC5
hsa-let-7a
1432.17
1422.63
2492.13
1352.32
1196.91
hsa-let-7b
5603.36
2448.27
3698.29
3766.39
3580.95
hsa-let-7c
293.72
138.95
360.04
200.70
547.20
hsa-let-7d
147.70
119.16
306.49
89.48
87.89
hsa-let-7e
120.12
82.25
345.64
79.65
17.58
hsa-let-7g
1573.21
1468.61
1776.82
929.83
863.15
hsa-let-7i
507.34
325.87
484.10
173.40
339.43
hsa-miR-001
38.40
0.00
0.00
0.00
0.00
hsa-miR-007
1096.74
662.71
284.21
222.43
671.49
hsa-miR-009
967.60
521.56
296.60
1557.24
704.42
hsa-miR-009*
122.00
82.11
36.76
126.10
95.61
hsa-miR-010a
688.40
218.31
780.56
443.15
406.95
hsa-miR-010b
45.67
3.63
16.82
24.24
5.05
hsa-miR-015a
706.67
553.66
735.54
300.12
559.98
hsa-miR-015b
7123.47
8131.90
9440.75
9040.04
5371.61
49138.68
36045.07
46396.61
38182.58
34757.70
hsa-miR-017-3p
37.99
49.39
4.85
7.13
28.89
hsa-miR-017-5p
1911.85
hsa-miR-016
1025.08
2600.63
1639.36
1354.59
hsa-miR-018
177.63
670.32
298.50
136.57
259.13
hsa-miR-019a
3530.79
4763.06
3088.77
2163.89
6580.42
hsa-miR-019b
16523.96
28827.05
12748.53
10503.62
27604.21
hsa-miR-020
22396.19
35690.95
18899.59
13421.80
32642.91
hsa-miR-021
9671.83
5293.54
26677.05
4331.44
7200.48
hsa-miR-022
288.38
340.80
429.22
328.03
240.48
hsa-miR-023a
125.31
228.76
167.66
63.60
152.89
hsa-miR-023b
65.99
42.61
455.77
123.00
314.85
hsa-miR-023bN
1103.36
1465.06
7551.85
2530.85
8578.99
hsa-miR-024
20496.76
35050.50
41223.35
25676.28
32990.65
hsa-miR-025
1060.01
719.49
1708.72
1088.65
1328.36
hsa-miR-026aN
7264.31
4701.94
19974.22
7103.69
5152.03
Continued
266
hsa-miR-026b
4445.90
3161.08
3952.33
3158.05
hsa-miR-026bN
2477.70
1654.49
2068.10
1370.63
757.74
hsa-miR-027a
4510.17
5304.75
3926.65
4333.81
6256.32
hsa-miR-027b
1648.16
256.93
332.92
2097.33
253.65
1835.57
hsa-miR-027bN
1627.44
2167.60
11174.64
2314.35
8291.54
hsa-miR-028
1228.88
676.82
861.53
1401.61
620.13
hsa-miR-029a
7498.57
3000.26
2691.27
2780.28
1103.67
hsa-miR-029b
505.15
94.42
201.56
55.28
73.88
hsa-miR-029c
684.04
252.28
300.06
224.58
162.53
hsa-miR-030a-3p
1524.75
708.62
1061.08
531.08
1373.86
hsa-miR-030c
3662.33
4989.50
14772.29
5544.51
6752.67
hsa-miR-030d
438.93
998.84
3123.83
496.58
528.68
hsa-miR-030e-3p
594.01
486.87
1380.50
512.99
527.08
hsa-miR-031
121187.86
36327.20
38963.94
86345.07
22803.02
hsa-miR-032
403.04
212.18
814.76
19.13
231.46
hsa-miR-033
0.00
0.00
14.58
0.00
0.00
hsa-miR-034a
hsa-miR-034aN
hsa-miR-034b
107.73
14.75
0.00
39.41
18.54
1330.28
169.80
149.74
523.74
311.56
20.13
8.24
25.96
8.18
28.65
hsa-miR-034b
11.27
0.00
15.90
0.00
0.00
hsa-miR-092N
11220.69
20022.99
9013.43
9347.40
16055.06
hsa-miR-093
12665.59
9682.32
22616.87
10025.99
13025.53
hsa-miR-095
43.28
14.41
210.56
191.81
100.70
hsa-miR-096
6325.35
13314.77
30352.78
13673.57
6370.14
hsa-miR-098
2038.54
69.87
351.55
241.84
358.11
hsa-miR-099a
994.25
5509.79
1182.19
522.35
800.10
hsa-miR-099b
1175.21
834.24
2918.64
1380.12
291.74
hsa-miR-100
930.03
6220.09
1076.12
508.77
530.13
hsa-miR-101
45.49
15.40
135.82
47.54
40.57
hsa-miR-103
603.50
754.20
1696.41
928.22
812.93
hsa-miR-105
16.52
15.54
0.00
11.04
22.15
hsa-miR-106a
1351.41
3260.90
3367.12
1902.95
1624.86
hsa-miR-106b
3416.05
1994.43
4845.71
1535.21
2606.35
hsa-miR-107
10.51
19.72
45.09
23.01
15.49
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
hsa-miR-125a
9683.18
4804.78
20089.22
10392.15
2211.89
hsa-miR-125b
762.80
2230.30
995.28
709.73
592.19
hsa-miR-126
2165.59
128.81
50.44
2911.92
1066.11
hsa-miR-126*
378.33
37.03
8.55
321.54
240.08
Continued
267
hsa-miR-127
0.00
2.16
0.00
0.00
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
hsa-miR-130a
1216.93
549.23
4377.06
926.57
403.38
hsa-miR-130aN
1165.90
3.18
2618.26
1817.11
9037.45
2229.60
hsa-miR-130b
498.16
833.29
1762.70
903.80
404.27
hsa-miR-132
906.37
606.72
821.18
772.87
1412.52
hsa-miR-133a
5.31
4.40
0.00
11.77
0.00
hsa-miR-134
3.30
32.36
2.38
3.98
2.43
hsa-miR-135a
128.17
67.08
41.32
60.66
139.43
hsa-miR-135b
4713.59
1646.60
1155.04
1361.54
1226.12
hsa-miR-138
3.96
15.74
11.60
13.30
33.44
hsa-miR-139
12.97
0.00
2.81
0.00
0.00
hsa-miR-140
572.76
532.56
2018.01
606.24
616.28
hsa-miR-141
1745.93
1128.41
6705.89
609.18
1476.14
hsa-miR-142-3p
5579.99
0.00
12.20
1205.81
11.78
hsa-miR-142-5p
710.77
6.20
4.28
417.58
6.37
hsa-miR-143
0.00
0.00
0.00
0.00
0.00
hsa-miR-145
27.71
6.78
10.18
104.36
22.46
hsa-miR-146
3137.52
728.77
373.72
7566.56
440.81
734.41
96.44
432.17
2268.08
263.53
0.00
0.00
0.00
0.00
0.00
673.64
543.52
382.81
1209.71
137.08
hsa-miR-146b
hsa-miR-147
hsa-miR-148a
hsa-miR-148b
132.87
227.23
427.84
172.71
158.73
hsa-miR-149
1794.34
1177.33
4151.14
2864.00
902.33
hsa-miR-150
21.57
7.81
6.80
50.90
85.35
hsa-miR-152
1661.66
407.09
2605.15
2011.11
1903.20
hsa-miR-152N
1643.86
450.69
2510.97
1376.50
1657.79
hsa-miR-153
0.00
0.00
0.00
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
hsa-miR-155
35.00
594.63
84.69
9.31
493.08
hsa-miR-181a
244.18
658.06
856.84
62.33
0.00
hsa-miR-181b
728.23
1607.38
2142.98
284.71
34.95
hsa-miR-181c
10.12
36.29
60.55
10.54
6.02
hsa-miR-182*
66.01
15.12
20.22
49.02
18.76
hsa-miR-183
1389.40
476.98
1006.88
585.86
577.57
hsa-miR-184
278.97
0.00
0.00
348.18
93.44
hsa-miR-186
2295.89
3763.10
7386.77
3147.66
1411.05
hsa-miR-187
0.00
20.06
16.89
2.41
0.00
hsa-miR-188
35.33
41.00
65.72
57.01
28.54
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
Continued
268
hsa-miR-191
5488.01
5080.40
6459.18
7471.67
hsa-miR-192
527.15
279.89
447.68
524.59
39.62
hsa-miR-193
366.97
18.44
24.30
385.56
126.24
hsa-miR-194
183.01
55.69
161.45
191.21
21.31
hsa-miR-195
734.89
434.85
984.56
917.31
483.57
hsa-miR-196a
1243.07
992.28
1331.75
2450.78
234.01
hsa-miR-196b
4164.59
393.29
30.67
17.39
174.89
81.36
1214.73
1423.20
3609.89
2525.99
1382.43
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
0.00
0.00
9.32
0.00
0.00
hsa-miR-199b
0.00
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
hsa-miR-200a
5893.33
4203.88
7628.01
2805.06
2977.62
hsa-miR-200b
10716.71
7665.11
10488.52
10176.51
6113.98
4480.53
3348.50
5424.39
3274.34
2516.38
hsa-miR-200c
18969.64
21459.45
86582.01
42338.19
26097.67
hsa-miR-200cN
hsa-miR-197
hsa-miR-200bN
27278.85
32710.20
84005.63
45245.42
26028.66
hsa-miR-203
3697.47
4213.54
5027.72
10178.17
6553.21
hsa-miR-204
0.00
0.00
4.51
0.00
0.00
hsa-miR-205
19002.31
9310.67
18948.25
12564.18
20304.15
hsa-miR-206
0.00
2.80
3.30
2.15
0.00
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
1746.62
3945.62
3858.69
4218.64
1330.00
hsa-miR-210N
2746.80
5989.76
7487.66
6202.59
2369.62
hsa-miR-211
2.94
0.00
0.00
0.00
0.00
hsa-miR-212
202.25
106.14
194.80
116.10
327.78
hsa-miR-213
0.00
59.56
6.06
0.00
0.00
hsa-miR-214
9.33
9.48
5.72
5.48
20.33
hsa-miR-215
4.50
8.91
9.30
0.00
12.19
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
3.36
0.00
0.00
0.00
0.00
hsa-miR-218
0.00
527.51
17.45
175.11
107.60
hsa-miR-219
0.00
0.00
0.00
0.00
0.00
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
hsa-miR-221
5263.74
2644.30
3883.90
4228.78
4612.91
hsa-miR-222
40414.60
17046.69
17770.14
29584.67
29111.49
hsa-miR-223
17.18
35.90
36.24
12.18
1.95
hsa-miR-296
397.27
2.30
46.66
416.68
2.45
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
hsa-miR-301
965.36
423.55
5180.45
653.93
959.68
0.00
0.00
0.00
0.00
0.00
hsa-miR-302a
Continued
269
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
35.45
56.31
165.84
38.44
24.41
hsa-miR-302c
0.00
20.78
0.00
0.00
14.19
hsa-miR-302c*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302d
3.69
0.00
0.00
0.00
11.90
hsa-miR-320
2693.86
3096.55
4396.66
3073.21
6590.27
hsa-miR-321
13650.16
7422.58
9823.46
4382.17
35515.52
hsa-miR-323
0.00
6.18
0.00
0.00
0.00
hsa-miR-324-3p
623.90
1325.04
2274.23
967.31
896.19
hsa-miR-324-5p
451.43
713.85
3064.81
586.92
657.07
hsa-miR-325
0.00
0.00
0.00
0.00
1.86
hsa-miR-326
0.00
6.14
0.00
7.37
1.91
hsa-miR-328
149.23
222.98
1325.51
292.55
159.77
hsa-miR-330
78.28
26.31
63.57
106.26
34.71
hsa-miR-331
2896.78
3477.73
6992.40
2849.81
2086.21
hsa-miR-335
678.78
53.76
150.98
216.08
264.45
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
17.60
0.00
0.00
20.20
0.00
hsa-miR-339
110.31
115.24
139.68
158.82
108.72
hsa-miR-340
85.94
89.40
169.57
147.71
36.48
hsa-miR-342
454.61
1204.99
1399.69
3457.64
1015.11
hsa-miR-345
656.94
1156.85
1468.44
1005.66
937.58
hsa-miR-346
6.19
6.25
10.59
3.84
35.88
hsa-miR-361
774.16
360.47
868.05
381.60
390.53
hsa-miR-367
0.00
0.00
0.00
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
100.75
73.24
107.84
123.66
105.54
hsa-miR-371
0.00
1.39
0.00
0.00
0.00
hsa-miR-372
179.95
185.15
139.27
141.50
285.58
hsa-miR-373
0.00
1.84
12.02
0.00
0.00
hsa-miR-374
1869.42
3226.98
5827.30
1695.29
2458.98
hsa-miR-375
262.59
74.29
59.06
228.98
29.78
hsa-miR-376a
3.96
112.36
10.29
9.48
0.00
hsa-miR-377
2.50
1.59
0.00
0.00
0.00
hsa-miR-378
81.93
59.30
35.74
110.11
35.37
hsa-miR-379
0.00
35.06
3.41
4.17
3.36
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
0.00
0.00
0.00
0.00
0.00
hsa-miR-381
0.00
0.00
15.44
0.00
5.61
hsa-miR-382
0.00
46.85
0.00
0.00
2.00
Continued
270
hsa-miR-383
0.00
0.00
0.00
0.00
hsa-miR-384
0.00
0.00
0.00
0.00
0.00
81.73
12.21
44.78
68.83
33.34
hsa-miR-423
391.07
727.64
1015.64
597.22
313.14
hsa-miR-425
277.88
139.92
467.20
245.33
171.92
0.00
0.00
0.00
0.00
0.00
hsa-miR-422b
let-7f(mmu)
miR-129-5p(mmu)
3.95
0.00
0.00
0.00
0.00
0.00
miR-137(mmu)
11.54
0.00
0.00
0.00
24.52
miR-224(mmu)
0.00
0.00
0.00
12.96
195.87
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
miRNA
T47D
Breast Cancer
MDA361
SKBR3
MCF7
MDA231
hsa-let-7a
1498.74
870.57
401.65
2045.14
648.65
hsa-let-7b
800.13
5424.95
3941.58
2922.04
246.17
hsa-let-7c
41.34
849.64
985.18
255.92
44.16
hsa-let-7d
0.00
108.15
84.79
317.25
0.00
hsa-let-7e
80.01
83.86
198.59
345.80
0.00
hsa-let-7g
153.64
642.89
459.41
1451.71
40.60
hsa-let-7i
58.12
150.71
73.43
988.90
66.23
hsa-miR-001
0.00
0.00
0.00
0.00
0.00
hsa-miR-007
192.74
169.83
42.46
1454.10
36.79
hsa-miR-009
52.04
567.34
16.49
635.36
19.44
hsa-miR-009*
6.09
52.86
9.64
128.30
0.00
hsa-miR-010a
580.48
0.00
38.64
0.00
101.22
hsa-miR-010b
9.16
0.00
0.00
0.00
0.00
hsa-miR-015a
757.25
159.85
129.69
761.34
32.44
hsa-miR-015b
11503.50
3795.32
7597.36
6613.15
245.00
hsa-miR-016
459.92
50925.02
19355.99
21337.19
49759.77
hsa-miR-017-3p
16.00
4.15
11.53
23.13
0.00
hsa-miR-017-5p
1346.56
772.00
287.62
395.16
70.06
hsa-miR-018
140.41
213.76
71.66
113.81
15.99
hsa-miR-019a
2442.86
3124.30
383.55
1777.67
143.49
hsa-miR-019b
11108.91
13834.15
2470.18
9249.32
817.59
hsa-miR-020
12836.43
13155.39
4057.42
13266.80
0.00
hsa-miR-021
2193.41
1127.54
2291.07
59172.65
89.45
hsa-miR-022
81.96
130.00
115.73
172.28
1.42
hsa-miR-023a
41.93
53.54
56.24
101.85
0.00
hsa-miR-023b
490.04
224.44
510.34
634.33
0.00
hsa-miR-023bN
hsa-miR-024
8771.84
2545.64
3509.36
4124.28
142.33
21049.93
18641.43
24760.84
35646.73
1696.98
Continued
271
hsa-miR-025
1066.59
1612.10
932.12
2989.40
174.42
hsa-miR-026aN
9812.92
3722.20
6215.36
14042.07
7254.63
hsa-miR-026b
3262.33
1513.86
1026.98
5462.80
45.09
hsa-miR-026bN
1757.11
884.11
588.70
3499.17
8.04
hsa-miR-027a
1551.66
1942.48
1037.34
3529.82
356.05
hsa-miR-027b
1867.36
717.08
293.92
1795.35
497.96
hsa-miR-027bN
1350.30
9042.91
5962.23
3991.23
16414.79
hsa-miR-028
344.29
274.84
461.54
507.43
39.61
hsa-miR-029a
321.57
993.01
246.18
355.78
266.59
10.11
hsa-miR-029b
58.18
44.49
17.67
36.38
hsa-miR-029c
584.63
587.96
114.50
374.06
8.63
hsa-miR-030a-3p
621.79
366.05
1409.36
138.52
161.50
hsa-miR-030c
6600.76
5742.83
9952.22
6981.41
1161.27
hsa-miR-030d
487.78
744.02
3694.70
2602.19
50.74
hsa-miR-030e-3p
556.03
726.45
370.48
444.66
32.28
hsa-miR-031
14.40
3050.21
691.74
10.32
376.38
hsa-miR-032
535.07
102.56
36.76
553.70
18.03
hsa-miR-033
6.44
0.00
0.00
0.00
0.00
hsa-miR-034a
55.67
36.31
37.94
127.03
0.00
hsa-miR-034aN
944.76
615.06
387.65
749.21
1.84
hsa-miR-034b
0.00
0.00
0.00
0.00
0.00
hsa-miR-034b
0.00
0.00
0.00
0.00
0.00
hsa-miR-092N
6523.59
9185.64
7370.18
8570.93
56.70
hsa-miR-093
14289.92
14076.43
13635.59
29148.70
0.00
hsa-miR-095
15.40
0.00
106.09
636.75
0.00
hsa-miR-096
12229.11
11180.21
14572.08
16948.46
158.78
hsa-miR-098
22.66
229.72
126.74
937.27
641.10
hsa-miR-099a
33.06
545.66
954.38
130.01
760.15
hsa-miR-099b
1894.27
826.25
5065.86
3324.39
93.83
hsa-miR-100
22.08
326.86
570.26
83.85
874.67
hsa-miR-101
69.45
14.05
18.00
148.07
0.00
hsa-miR-103
1930.92
521.98
944.27
1737.23
11.89
hsa-miR-105
13.27
5.84
0.00
0.00
17.22
hsa-miR-106a
1775.25
1345.88
704.82
1511.32
19.76
hsa-miR-106b
2922.91
2634.66
958.85
5376.53
77.73
hsa-miR-107
41.28
11.82
9.80
34.00
0.00
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
hsa-miR-125a
10610.36
5907.63
34298.11
21630.77
1109.70
hsa-miR-125b
33.88
754.41
1355.85
183.70
1162.40
Continued
272
hsa-miR-126
296.52
115.01
652.26
1483.07
0.00
hsa-miR-126*
94.77
49.87
81.12
259.14
0.00
hsa-miR-127
0.00
0.00
380.84
0.00
0.00
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
hsa-miR-130a
1667.75
552.09
152.62
2.98
1.93
hsa-miR-130aN
3782.08
1669.05
561.97
4.77
23.25
hsa-miR-130b
534.90
338.02
411.64
393.53
14.85
hsa-miR-132
409.01
117.11
344.49
1003.90
172.12
0.00
0.00
0.00
0.00
0.00
11.48
hsa-miR-133a
hsa-miR-134
1.48
3.42
15.09
7.82
hsa-miR-135a
202.54
0.00
10.98
1079.76
8.87
hsa-miR-135b
37.65
44.11
119.70
311.68
34.75
0.00
2.26
1.77
3.85
7.95
hsa-miR-138
hsa-miR-139
0.00
0.00
0.00
13.33
0.00
hsa-miR-140
555.92
546.61
256.60
857.85
244.23
hsa-miR-141
625.98
1938.85
1032.20
3096.63
0.00
hsa-miR-142-3p
3.33
0.00
0.00
7.90
0.00
hsa-miR-142-5p
5.84
1.69
4.69
4.41
2.78
hsa-miR-143
0.00
0.00
0.00
12.89
0.00
hsa-miR-145
0.00
14.79
21.02
19.61
9.36
hsa-miR-146
405.51
333.55
380.21
311.33
826.08
hsa-miR-146b
289.02
121.45
57.52
242.47
66.87
hsa-miR-147
0.00
0.00
0.00
0.00
0.00
hsa-miR-148a
871.04
544.88
688.91
395.60
0.00
hsa-miR-148b
612.02
127.35
233.78
356.35
4.82
hsa-miR-149
1990.84
1286.17
3107.17
1435.43
76.43
hsa-miR-150
20.77
0.00
10.39
24.03
0.00
hsa-miR-152
704.23
13.14
126.31
464.24
45.17
hsa-miR-152N
749.04
59.42
157.93
550.59
68.70
hsa-miR-153
0.00
0.00
0.00
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
hsa-miR-155
15.46
11.26
0.00
17.81
53.85
hsa-miR-181a
934.44
819.13
1631.80
656.42
20.37
hsa-miR-181b
2442.65
2895.16
2575.19
2071.75
199.51
hsa-miR-181c
63.46
39.55
80.90
43.53
2.84
hsa-miR-182*
27.25
29.12
24.42
27.31
3.14
hsa-miR-183
670.02
496.59
927.99
1218.41
11.74
hsa-miR-184
97.66
118.77
100.52
31.45
0.00
hsa-miR-186
2931.40
3726.12
1629.45
1783.62
304.48
hsa-miR-187
204.74
0.00
172.97
12.78
0.00
Continued
273
hsa-miR-188
44.57
50.33
34.74
29.62
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
hsa-miR-191
10861.30
7001.03
27487.01
8542.76
460.62
hsa-miR-192
848.53
175.86
302.99
694.20
57.61
hsa-miR-193
647.36
324.80
420.16
661.17
22.20
hsa-miR-194
203.98
29.93
169.07
241.10
12.49
hsa-miR-195
42.15
934.69
359.98
228.29
2533.98
30.24
hsa-miR-196a
11284.40
807.33
1718.36
122.02
32.91
hsa-miR-196b
2.11
0.00
0.00
0.00
0.00
476.30
hsa-miR-197
1618.67
1485.51
3025.34
1677.30
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
16.86
11.00
0.00
9.21
0.00
hsa-miR-199b
0.00
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
hsa-miR-200a
4873.52
10.30
1966.60
5197.61
0.00
hsa-miR-200b
8457.67
4.90
7128.69
7749.47
0.00
hsa-miR-200bN
3880.92
103.03
5200.34
5423.04
9.62
hsa-miR-200c
13019.23
32384.49
88921.49
32197.20
0.00
hsa-miR-200cN
11492.03
29978.08
70071.61
29302.55
4.20
hsa-miR-203
8507.06
459.16
2229.81
10460.21
0.00
hsa-miR-204
0.00
0.00
0.00
0.00
24.60
hsa-miR-205
1801.77
93.86
6731.92
52.70
0.00
hsa-miR-206
4.53
0.00
12.27
0.00
0.00
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
942.76
589.61
1599.54
505.32
71.07
142.42
hsa-miR-210N
1533.71
869.99
2284.77
958.25
hsa-miR-211
0.00
0.00
0.00
22.34
0.00
hsa-miR-212
154.95
120.80
222.56
236.86
140.39
hsa-miR-213
12.18
126.10
56.35
5.03
4.21
hsa-miR-214
12.83
12.51
3.89
9.47
5.24
hsa-miR-215
11.86
0.00
11.79
0.00
0.00
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
0.00
0.00
0.00
0.00
0.00
hsa-miR-218
9.63
0.00
0.00
277.06
0.00
hsa-miR-219
0.00
0.00
0.00
0.00
0.00
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
hsa-miR-221
0.00
2.48
0.00
139.68
201.49
hsa-miR-222
6.59
425.70
98.30
1154.48
5827.27
hsa-miR-223
0.00
5.26
1.58
44.65
0.00
hsa-miR-296
757.33
522.24
34.03
1738.66
386.05
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
Continued
274
hsa-miR-301
4943.87
218.46
1209.26
8225.62
22.28
hsa-miR-302a
0.00
0.00
0.00
0.00
0.00
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
42.02
41.53
16.65
30.49
53.60
hsa-miR-302c
0.00
61.26
0.00
0.00
0.00
hsa-miR-302c*
0.00
0.00
0.00
0.00
0.00
hsa-miR-302d
0.00
0.00
1.56
10.03
0.00
hsa-miR-320
4970.52
1722.84
2372.43
3364.47
928.48
hsa-miR-321
5745.97
5749.84
17712.84
9302.29
1408.56
hsa-miR-323
0.00
0.00
2.02
0.00
0.00
hsa-miR-324-3p
1271.33
114.67
1223.51
1176.52
139.26
hsa-miR-324-5p
1205.83
55.49
628.77
1225.76
55.51
0.00
0.00
0.00
0.00
0.00
hsa-miR-325
hsa-miR-326
15.03
0.00
7.00
22.72
0.00
hsa-miR-328
991.98
30.03
1468.84
347.30
49.42
hsa-miR-330
32.76
5.43
40.30
41.67
0.00
hsa-miR-331
3125.21
2410.47
13185.67
2953.99
56.15
hsa-miR-335
0.00
512.36
47.39
399.00
0.00
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
0.00
0.00
0.00
0.00
0.00
hsa-miR-339
133.91
94.38
172.69
214.64
5.18
hsa-miR-340
201.75
76.90
128.21
206.35
15.24
hsa-miR-342
2258.91
794.08
1871.69
20000.33
121.53
hsa-miR-345
1429.28
611.30
2285.66
4000.28
29.30
hsa-miR-346
8.37
8.01
16.52
49.75
1.89
hsa-miR-361
191.93
95.40
88.81
249.58
16.46
hsa-miR-367
0.00
0.00
0.00
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
67.96
79.56
173.90
138.53
60.16
hsa-miR-371
0.00
0.00
0.00
0.00
0.00
hsa-miR-372
223.30
117.62
127.50
231.01
68.32
hsa-miR-373
0.00
0.00
0.00
0.00
4.47
hsa-miR-374
3029.43
2150.67
803.59
3746.70
11.82
hsa-miR-375
2889.81
470.68
21466.35
1474.46
4.85
hsa-miR-376a
0.00
1.45
18.66
0.00
0.00
hsa-miR-377
0.00
0.00
0.00
0.00
0.00
hsa-miR-378
20.59
57.74
81.89
36.93
23.44
hsa-miR-379
0.00
2.68
21.74
0.00
0.00
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
0.00
0.00
0.00
0.00
0.00
Continued
275
hsa-miR-381
0.00
0.00
4.90
0.00
17.70
hsa-miR-382
0.00
0.00
11.98
0.00
0.00
hsa-miR-383
0.00
0.00
0.00
16.39
0.00
hsa-miR-384
0.00
0.00
0.00
0.00
0.00
29.84
108.27
28.79
47.56
25.81
hsa-miR-423
708.68
590.66
1283.90
416.90
95.42
hsa-miR-425
hsa-miR-422b
704.22
241.59
943.45
421.79
29.72
let-7f(mmu)
0.00
0.00
0.00
0.00
0.00
miR-129-5p(mmu)
0.00
0.00
0.00
0.00
0.00
miR-137(mmu)
0.00
0.00
0.00
0.00
0.00
miR-224(mmu)
0.00
91.37
0.00
0.00
0.00
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
miRNA
HCT8
Colorectal Cancer
HCT116
HT29
SW620
HCT15
HeLa
hsa-let-7a
2981.59
183.91
1458.70
1146.77
1994.70
97.64
hsa-let-7b
14540.76
679.83
4177.99
4045.54
18455.59
3004.33
hsa-let-7c
650.74
34.35
237.37
297.57
733.22
347.79
hsa-let-7d
130.88
13.11
237.52
131.11
84.33
9.31
hsa-let-7e
226.88
7.58
247.92
45.25
151.88
5.55
hsa-let-7g
1671.78
136.22
929.23
1390.01
2045.86
23.38
hsa-let-7i
164.52
183.71
102.34
429.32
279.31
257.20
hsa-miR-001
13.65
0.00
0.00
49.13
0.00
0.00
hsa-miR-007
302.92
124.84
2469.58
3391.31
955.96
0.00
hsa-miR-009
31.67
254.87
5.36
3332.02
15.83
0.00
hsa-miR-009*
18.39
45.33
0.00
594.67
0.00
1.37
hsa-miR-010a
11539.35
231.93
6261.34
3321.67
5038.28
0.00
hsa-miR-010b
3273.00
4.93
1815.59
1343.14
435.07
0.00
hsa-miR-015a
387.64
0.00
441.85
493.21
694.29
17.46
hsa-miR-015b
4008.22
394.29
5725.16
6057.67
4583.43
493.58
29240.66
2019.13
22778.48
36400.59
31816.51
57.90
hsa-miR-016
hsa-miR-017-3p
36.34
0.00
42.81
16.83
11.80
0.00
hsa-miR-017-5p
2861.47
279.37
2015.85
4931.14
3440.33
27.67
281.96
43.23
798.21
461.67
357.08
1.50
hsa-miR-018
hsa-miR-019a
5080.70
812.14
8003.93
14387.34
7672.47
0.00
hsa-miR-019b
25484.30
3165.02
34546.51
66636.93
37604.17
22.80
hsa-miR-020
34241.85
4357.09
44883.87
80463.19
50806.43
161.35
hsa-miR-021
1676.77
1527.42
4011.47
3527.59
4460.74
22.00
hsa-miR-022
66.12
0.00
117.41
9.13
81.13
0.00
hsa-miR-023a
18.71
11.27
32.92
55.58
118.78
8.98
hsa-miR-023b
132.36
30.39
190.24
422.68
68.79
0.00
Continued
276
hsa-miR-023bN
1765.34
247.55
2098.77
11575.28
1745.85
233.63
hsa-miR-024
12999.89
2529.96
26074.78
36341.93
28448.54
1250.05
hsa-miR-025
963.37
419.84
2070.35
3664.79
1113.67
35.63
hsa-miR-026aN
10395.19
925.23
4996.18
9947.63
6718.07
79.77
hsa-miR-026b
3304.79
229.07
3109.24
3602.51
4491.24
20.15
hsa-miR-026bN
1605.33
119.74
1635.25
1952.60
2239.18
10.79
hsa-miR-027a
1372.15
529.44
1644.03
2344.51
4434.74
46.14
hsa-miR-027b
531.21
20.88
486.81
2608.71
409.93
17.39
3220.54
160.89
3748.76
11881.83
1981.29
169.79
hsa-miR-027bN
hsa-miR-028
305.87
48.73
642.32
440.15
315.05
0.00
hsa-miR-029a
3957.26
809.99
1976.32
3391.55
3048.37
20.87
hsa-miR-029b
170.26
36.07
85.41
96.66
159.85
0.00
hsa-miR-029c
293.55
88.99
229.72
204.47
294.84
0.00
hsa-miR-030a-3p
0.00
0.00
0.00
39.94
108.86
108.19
hsa-miR-030c
6404.44
1147.38
5324.90
6262.14
4542.52
123.32
hsa-miR-030d
674.24
107.79
2021.45
633.55
271.64
10.93
hsa-miR-030e-3p
750.43
46.13
311.19
833.51
546.77
3.63
hsa-miR-031
3214.75
17316.78
48373.34
3645.68
8322.72
340.26
hsa-miR-032
208.14
40.98
153.82
152.39
276.81
0.00
hsa-miR-033
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-034a
62.82
0.00
12.82
4.08
19.58
19.38
753.46
156.77
186.64
101.05
280.87
65.99
hsa-miR-034b
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-034b
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-092N
18967.69
2405.82
23341.01
54271.90
21328.96
4638.85
hsa-miR-093
16206.79
2448.54
23278.79
34278.99
17925.49
751.21
hsa-miR-095
346.78
75.88
22.14
391.30
136.75
24.34
hsa-miR-096
17398.44
12454.29
10421.57
11006.18
37633.61
48346.58
hsa-miR-098
216.04
29.24
492.65
323.15
348.71
0.00
hsa-miR-099a
198.10
2012.21
106.30
1461.20
92.11
59.50
hsa-miR-099b
825.85
302.29
2126.07
559.74
1524.52
42.66
hsa-miR-100
158.95
2342.43
118.76
1604.34
54.61
25.35
hsa-miR-034aN
hsa-miR-101
38.32
9.03
62.66
81.59
56.34
0.00
hsa-miR-103
550.54
147.47
1362.73
1288.98
1002.92
10.91
hsa-miR-105
0.00
0.00
0.00
0.00
2.77
0.00
hsa-miR-106a
3683.00
995.85
2439.15
4614.12
5110.52
2901.07
hsa-miR-106b
1880.20
449.59
3230.59
3480.99
2940.59
18.04
hsa-miR-107
20.30
0.00
16.55
42.79
0.00
0.00
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
0.00
Continued
277
hsa-miR-125a
8212.77
1859.42
10620.96
4116.32
12708.71
887.81
hsa-miR-125b
0.00
2224.88
20.36
831.65
29.05
227.86
hsa-miR-126
938.73
314.39
37.38
1098.62
5.78
0.00
hsa-miR-126*
636.12
234.70
78.21
751.68
3.53
8.09
hsa-miR-127
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-130a
164.04
81.53
101.90
258.04
8.58
2.76
18.92
150.94
21.83
60.03
2.17
26.95
hsa-miR-130b
817.38
148.94
473.50
1081.93
968.71
11.27
hsa-miR-132
hsa-miR-130aN
441.82
142.27
307.82
1863.09
651.60
70.17
hsa-miR-133a
4.58
0.00
0.00
25.14
0.00
8.48
hsa-miR-134
9.65
3.84
14.04
9.82
7.06
8.29
hsa-miR-135a
28.33
4.93
46.85
494.73
9.42
0.00
hsa-miR-135b
771.14
295.36
1244.98
2450.92
226.45
0.00
hsa-miR-138
0.00
0.00
10.86
4.83
1.93
0.00
hsa-miR-139
12.36
4.08
0.00
0.00
0.00
0.00
hsa-miR-140
1611.25
170.68
696.76
2626.31
2319.61
0.00
hsa-miR-141
1232.71
338.65
1274.09
26.59
1291.73
0.00
hsa-miR-142-3p
227.84
0.00
198.21
5492.13
77.41
0.00
hsa-miR-142-5p
58.04
7.35
49.78
1692.60
15.67
2.71
hsa-miR-143
3.16
0.00
0.00
2.19
0.00
0.00
hsa-miR-145
6.95
28.55
7.99
20.89
6.55
6.57
hsa-miR-146
462.04
302.07
885.46
665.53
392.93
349.86
2911.73
90.27
143.77
500.11
293.38
17.26
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-148a
792.39
94.73
69.32
926.29
1169.72
0.00
hsa-miR-148b
223.71
49.20
232.68
286.83
245.40
0.00
hsa-miR-149
330.27
267.94
177.26
1177.31
704.51
32.52
hsa-miR-150
7.95
22.21
350.76
232.74
23.03
17.93
hsa-miR-152
490.61
19.04
164.36
685.59
565.02
32.53
hsa-miR-152N
512.91
62.09
220.70
442.64
474.89
26.82
hsa-miR-153
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-155
36.25
2.38
146.31
7.51
552.12
52.39
hsa-miR-146b
hsa-miR-147
hsa-miR-181a
4.37
29.48
564.03
663.50
58.26
115.06
hsa-miR-181b
115.39
153.53
1929.59
2033.60
256.72
223.55
hsa-miR-181c
1.99
4.03
37.81
76.67
17.59
7.03
12.38
hsa-miR-182*
19.92
13.41
24.77
16.20
25.62
hsa-miR-183
427.97
97.01
419.13
914.00
627.03
2.10
hsa-miR-184
0.00
1.48
0.00
0.00
14.35
0.00
Continued
278
hsa-miR-186
1822.96
861.05
5015.29
3939.76
3434.66
hsa-miR-187
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-188
18.45
38.05
47.61
51.40
30.78
32.45
21.57
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-191
3821.39
1563.03
3664.16
5584.09
10469.55
291.06
hsa-miR-192
4407.85
22.58
24270.80
14008.19
933.89
0.00
hsa-miR-193
178.07
26.59
42.27
6.05
38.45
9.63
hsa-miR-194
1218.36
6.71
5196.53
3638.52
292.06
0.00
hsa-miR-195
299.43
28.62
189.76
261.73
375.13
1.81
hsa-miR-196a
1304.06
134.34
2638.79
3410.42
1153.35
228.79
hsa-miR-196b
0.00
0.00
395.26
265.11
16.98
0.00
1241.78
1254.49
1769.50
1904.93
1980.97
292.87
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
9.67
0.00
6.85
8.74
12.62
0.00
hsa-miR-199b
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-197
hsa-miR-200a
3803.53
667.47
14210.01
2946.38
4976.87
hsa-miR-200b
11575.51
599.72
40770.61
10992.02
12333.80
0.00
3800.74
278.08
13412.99
3434.33
3696.94
0.00
hsa-miR-200c
26716.43
4957.90
23494.64
1106.80
35698.53
1.46
hsa-miR-200cN
22284.01
3550.53
24454.01
667.17
39036.64
0.00
hsa-miR-203
9763.52
343.04
3009.16
831.61
6205.45
13.62
hsa-miR-204
0.00
0.00
0.00
921.30
48.75
4.78
hsa-miR-205
0.00
0.00
79.10
13.53
0.00
0.00
hsa-miR-206
3.96
2.07
8.99
1.50
0.00
1.43
0.00
hsa-miR-200bN
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
913.12
116.36
538.88
333.63
858.50
5.70
1498.52
248.57
713.75
490.69
1558.61
29.03
hsa-miR-211
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-212
209.55
186.29
145.47
329.65
203.79
73.62
hsa-miR-213
0.00
2.26
40.38
88.13
2.86
0.00
hsa-miR-214
5.26
6.78
10.12
3.93
0.00
6.06
hsa-miR-215
39.17
0.00
139.59
26.67
15.20
0.00
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
7.64
0.00
0.00
0.00
1.42
0.00
hsa-miR-218
0.00
19.45
0.00
51.08
1.41
0.00
hsa-miR-219
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-210N
hsa-miR-221
1607.73
1101.84
3639.45
3074.69
2441.29
9.91
hsa-miR-222
13748.91
14902.59
19900.07
19247.57
20363.61
730.06
hsa-miR-223
0.00
0.00
0.00
0.00
0.00
21.54
Continued
279
hsa-miR-296
18.71
22.47
5.71
5.32
2.79
94.50
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-301
642.38
201.45
1388.28
842.44
1467.44
0.00
hsa-miR-302a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
7.78
4.95
1.54
6.39
108.25
34.35
hsa-miR-302c
0.00
34.38
0.00
0.00
24.26
0.00
hsa-miR-302c*
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-302d
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-320
4707.69
1193.35
1854.40
7736.92
6465.03
881.54
hsa-miR-321
6516.35
1613.15
18992.34
8820.76
6827.65
505.53
hsa-miR-323
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-324-3p
285.05
127.77
483.67
1513.06
1430.63
122.48
hsa-miR-324-5p
640.68
155.76
449.69
542.14
906.36
8.14
hsa-miR-325
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-326
7.15
2.11
5.08
2.21
9.05
0.00
hsa-miR-328
226.63
135.11
59.95
791.33
178.80
46.52
hsa-miR-330
39.71
15.17
56.23
29.63
61.11
1.76
hsa-miR-331
1673.72
778.22
2668.59
2437.66
2157.33
321.60
hsa-miR-335
351.12
21.95
324.67
1093.08
184.04
0.00
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
0.00
0.00
106.59
0.00
9.19
0.00
10.02
hsa-miR-339
83.50
28.73
78.38
108.15
41.52
hsa-miR-340
124.51
34.50
108.95
180.35
89.00
0.00
hsa-miR-342
0.00
176.07
0.00
13.76
0.00
43.99
hsa-miR-345
723.00
207.45
380.66
765.68
600.24
19.54
hsa-miR-346
10.11
13.85
20.02
33.91
8.20
27.85
hsa-miR-361
242.91
62.41
256.46
596.96
267.64
4.41
hsa-miR-367
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
92.82
80.39
102.63
44.87
57.94
85.25
hsa-miR-371
106.77
0.00
0.00
0.00
507.15
0.00
hsa-miR-372
602.69
99.64
84.52
264.02
2785.91
129.72
hsa-miR-373
31.48
0.00
0.00
3.39
288.91
0.00
hsa-miR-374
3849.70
417.93
5233.17
6911.48
1880.54
0.00
hsa-miR-375
1217.73
124.35
3433.88
23411.10
4933.26
0.00
0.00
0.00
5.90
2.29
7.15
0.00
hsa-miR-376a
hsa-miR-377
5.68
0.00
1.99
9.73
1.87
0.00
hsa-miR-378
102.18
22.53
74.04
36.02
91.66
12.17
hsa-miR-379
1.77
0.00
2.31
0.00
0.00
0.00
Continued
280
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
1.53
0.00
0.00
0.00
0.00
0.00
hsa-miR-381
0.00
7.22
0.00
3.45
0.00
0.00
hsa-miR-382
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-383
0.00
0.00
0.00
0.00
3.58
0.00
hsa-miR-384
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-422b
76.55
26.23
7.23
40.17
44.74
2.56
hsa-miR-423
411.15
194.95
398.49
467.78
470.12
288.90
hsa-miR-425
183.07
55.19
227.40
314.96
759.80
12.15
let-7f(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
miR-129-5p(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
miR-137(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
miR-224(mmu)
8.52
0.00
144.45
7.48
8.60
3.95
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
miRNA
RAMOS
Hematological Cancer
HL60
K562
JURCAT
DAUDI
293T
hsa-let-7a
476.24
1227.07
158.91
1561.33
257.93
576.04
hsa-let-7b
170.17
1327.88
18.92
2422.87
180.41
199.06
hsa-let-7c
395.07
149.41
0.00
143.65
95.66
330.75
hsa-let-7d
117.48
168.89
0.00
201.13
70.05
0.00
hsa-let-7e
0.00
5.02
12.18
20.57
0.00
46.84
hsa-let-7g
3023.14
2037.42
46.67
2713.04
1840.27
546.74
hsa-let-7i
334.50
261.92
36.01
479.18
302.21
70.98
hsa-miR-001
0.00
0.00
0.00
4.28
0.00
41.13
hsa-miR-007
231.19
78.48
83.06
2271.12
501.25
871.54
hsa-miR-009
116.83
30.74
833.69
3556.40
432.26
1892.64
hsa-miR-009*
40.55
3.27
272.19
698.75
166.10
468.84
hsa-miR-010a
0.00
0.00
669.55
0.00
5.80
2487.12
hsa-miR-010b
0.00
0.00
13.70
0.00
0.00
749.90
hsa-miR-015a
1160.95
697.23
252.42
1735.77
231.07
783.29
hsa-miR-015b
8136.28
5490.35
6712.70
9037.28
2819.87
6407.23
100287.15
35183.56
27742.35
86180.90
42795.43
43217.17
hsa-miR-016
hsa-miR-017-3p
209.81
78.37
123.85
96.83
176.82
135.05
hsa-miR-017-5p
2391.84
4252.95
8317.35
6578.31
2424.41
8712.46
hsa-miR-018
748.84
601.23
2138.55
1502.74
541.72
1418.87
hsa-miR-019a
15383.65
10144.15
27202.80
17315.74
11819.43
21531.63
hsa-miR-019b
64653.48
57911.03
105820.80
82056.41
63322.49
88132.29
hsa-miR-020
80036.65
70308.31
140864.52
105847.92
68168.51
73186.00
hsa-miR-021
330.54
349.04
4476.93
699.20
682.04
439.93
hsa-miR-022
6.82
0.00
101.16
29.17
7.67
100.80
Continued
281
hsa-miR-023a
0.00
94.37
25.58
4.68
0.00
hsa-miR-023b
22.05
32.95
23.53
40.98
0.00
28.69
150.68
606.89
668.25
933.75
11.35
1156.84
hsa-miR-024
1205.61
16971.70
7051.43
3361.53
269.19
3258.01
hsa-miR-025
2553.96
2558.49
3451.85
3218.04
722.52
2667.26
hsa-miR-026aN
4750.03
3081.36
5040.04
5105.37
1200.29
4822.47
hsa-miR-026b
1480.03
3230.85
2766.01
3245.93
734.25
2257.01
hsa-miR-023bN
hsa-miR-026bN
15.10
829.46
1418.85
1468.50
1428.07
511.42
1128.64
hsa-miR-027a
54.02
3629.05
1092.28
552.12
34.25
432.68
hsa-miR-027b
0.00
84.69
127.77
206.60
14.87
355.65
1482.78
562.68
476.28
1373.40
279.75
2299.56
hsa-miR-028
201.68
145.03
0.00
647.47
442.10
227.69
hsa-miR-029a
2085.19
525.96
343.58
1043.35
1192.86
422.56
hsa-miR-029b
24.98
32.59
0.00
74.82
24.95
38.42
hsa-miR-029c
276.03
298.80
92.05
2086.16
532.72
272.20
20.97
0.00
2.36
0.00
0.00
762.23
hsa-miR-030c
5574.80
7402.57
6113.83
9477.76
3176.38
7134.64
hsa-miR-030d
1476.87
561.82
1004.18
832.28
1050.14
587.32
hsa-miR-027bN
hsa-miR-030a-3p
hsa-miR-030e-3p
420.02
861.02
876.56
1050.16
354.99
677.80
hsa-miR-031
1.88
0.00
0.00
8.27
38.13
5053.65
hsa-miR-032
359.92
227.02
487.27
934.25
94.47
598.02
hsa-miR-033
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-034a
0.00
0.00
0.00
0.00
0.00
95.07
hsa-miR-034aN
2.24
0.00
11.49
8.76
0.00
939.42
hsa-miR-034b
0.00
0.00
0.00
0.00
0.00
10.87
hsa-miR-034b
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-092N
36634.07
38856.53
76696.29
45585.33
29405.83
49278.04
hsa-miR-093
12102.83
22405.08
16757.50
32796.90
2432.46
18029.98
hsa-miR-095
46.43
0.00
0.00
58.53
57.31
228.99
hsa-miR-096
16954.20
10215.68
15049.70
12537.86
14053.04
15450.54
hsa-miR-098
34.67
167.95
0.00
782.04
7.91
68.43
hsa-miR-099a
630.48
158.16
17.61
10.08
379.06
528.84
hsa-miR-099b
0.00
6.74
714.62
134.99
3.75
659.87
hsa-miR-100
255.47
65.90
11.69
7.33
206.41
318.65
hsa-miR-101
101.93
62.14
109.87
84.69
43.61
100.83
hsa-miR-103
406.14
736.39
478.35
1212.18
189.56
1100.61
hsa-miR-105
0.00
0.00
32.45
0.00
8.16
0.00
hsa-miR-106a
2529.12
3257.09
4641.96
5226.03
2456.19
7592.53
hsa-miR-106b
5421.12
3804.48
3161.85
6202.17
1406.73
3724.92
hsa-miR-107
0.00
18.08
14.58
20.62
0.00
31.43
hsa-miR-108
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-122a
0.00
0.00
0.00
0.00
0.00
0.00
Continued
282
hsa-miR-124a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-125a
0.00
160.74
5429.93
906.74
22.52
3792.74
hsa-miR-125b
710.54
133.81
0.00
0.00
303.84
850.49
hsa-miR-126
0.00
18.49
15649.06
849.30
0.00
1462.15
hsa-miR-126*
4.89
21.32
3037.39
170.97
0.00
253.11
hsa-miR-127
0.00
0.00
35.34
0.00
0.00
12.45
hsa-miR-128a
0.00
0.00
0.00
0.00
0.00
0.00
27.99
hsa-miR-130a
5.15
0.00
54.28
29.04
4.60
20.92
1.52
6.86
46.51
22.27
2.61
495.88
200.07
2751.96
1735.27
294.28
897.40
hsa-miR-132
79.16
45.56
525.33
724.73
563.68
1058.09
hsa-miR-133a
5.19
5.35
0.00
0.00
0.00
8.31
hsa-miR-134
1.82
3.47
20.45
5.54
4.74
7.27
hsa-miR-135a
0.00
8.35
29.26
174.93
0.00
0.00
hsa-miR-130aN
hsa-miR-130b
hsa-miR-135b
0.00
0.00
241.70
3.28
0.00
58.73
hsa-miR-138
52.05
0.00
0.00
5.76
6.46
13.15
hsa-miR-139
0.00
0.00
0.00
11.35
0.00
7.83
hsa-miR-140
2199.51
2571.88
1993.32
1951.24
828.19
1597.67
hsa-miR-141
0.00
5.81
4.53
49.08
0.00
5.98
hsa-miR-142-3p
19725.56
23664.42
12437.70
40066.70
13900.74
15.09
hsa-miR-142-5p
2571.31
3874.12
1784.88
6533.13
1367.77
4.79
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-143
hsa-miR-145
6.10
24.56
51.85
25.44
2.65
6.79
hsa-miR-146
11941.26
1403.02
537.84
892.52
865.85
552.42
286.39
214.77
17729.83
312.77
308.05
470.55
hsa-miR-146b
hsa-miR-147
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-148a
1558.38
917.15
1176.32
2484.78
181.01
2497.30
hsa-miR-148b
94.85
188.80
141.11
242.27
33.68
298.74
hsa-miR-149
7.98
5.61
10.98
176.83
19.14
772.47
hsa-miR-150
992.51
23.18
0.00
5179.88
866.32
12.31
hsa-miR-152
199.34
4.35
47.43
127.16
38.36
275.01
hsa-miR-152N
185.95
29.66
40.18
154.50
66.98
369.07
hsa-miR-153
0.00
0.00
0.00
8.89
0.00
0.00
hsa-miR-154
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-154*
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-155
31.61
434.48
6.13
5.23
337.71
6.95
hsa-miR-181a
1060.60
3933.94
0.00
6586.14
178.40
474.63
hsa-miR-181b
2127.41
6839.53
58.32
17286.88
899.36
938.48
hsa-miR-181c
59.83
163.01
0.00
342.21
15.41
57.96
hsa-miR-182*
2.80
0.00
19.09
22.36
0.00
21.75
hsa-miR-183
82.41
2.48
480.61
687.52
2.16
354.98
hsa-miR-184
0.00
0.00
0.00
86.69
30.59
18.03
Continued
283
hsa-miR-186
1551.68
5441.12
4549.78
6923.61
621.71
7705.67
hsa-miR-187
0.00
0.00
0.00
11.67
0.00
212.58
hsa-miR-188
20.91
27.89
26.29
19.80
21.06
66.72
hsa-miR-190
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-191
9349.85
14701.60
8680.03
16887.18
1467.62
5974.20
hsa-miR-192
259.75
70.63
480.52
575.28
389.65
830.94
hsa-miR-193
10.26
25.09
0.00
42.78
17.20
38.91
260.42
hsa-miR-194
65.60
26.11
143.87
180.91
132.32
hsa-miR-195
926.49
299.42
295.38
724.90
336.23
853.43
hsa-miR-196a
0.00
14.47
167.16
954.39
35.22
4465.57
hsa-miR-196b
0.00
0.00
0.00
0.00
0.00
2007.48
3281.06
hsa-miR-197
638.13
2129.18
2248.85
1245.66
211.17
hsa-miR-199a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-199a*
0.00
327.50
23.91
5.54
19.34
11.94
hsa-miR-199b
0.00
35.34
0.00
0.00
0.00
0.00
hsa-miR-199-s
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-200a
1.97
0.00
0.00
0.00
0.00
7.08
hsa-miR-200b
7.85
8.27
8.68
9.27
0.00
23.33
hsa-miR-200bN
18.19
0.00
3.20
2.44
0.00
4.80
hsa-miR-200c
19.18
68.93
99.69
606.65
28.55
187.68
hsa-miR-200cN
23.80
57.47
75.76
512.11
0.00
157.62
hsa-miR-203
8.44
0.00
0.00
221.64
0.00
18.43
hsa-miR-204
2.20
0.00
3.87
0.00
0.00
121.80
hsa-miR-205
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-206
4.54
0.00
0.00
6.68
3.28
0.00
hsa-miR-208
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-210
569.34
7.26
243.67
314.03
96.74
1119.41
hsa-miR-210N
924.90
46.43
462.86
511.37
250.23
1893.90
hsa-miR-211
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-212
125.79
79.52
215.32
167.87
114.88
217.90
hsa-miR-213
20.63
601.11
0.00
994.57
14.24
34.27
hsa-miR-214
9.22
2.12
4.80
2.85
19.88
14.66
hsa-miR-215
0.00
0.00
38.78
0.00
0.00
11.14
hsa-miR-216
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-217
0.00
0.00
9.58
0.00
0.00
0.00
hsa-miR-218
0.00
6.52
147.02
30.88
0.00
4230.55
hsa-miR-219
0.00
0.00
0.00
0.00
0.00
5.95
hsa-miR-220
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-221
0.00
2604.05
146.04
846.12
0.00
8228.70
hsa-miR-222
15.66
12446.45
928.58
4983.01
377.84
46265.29
hsa-miR-223
14.40
165722.35
73928.30
19663.34
48.10
0.00
hsa-miR-296
361.39
9.52
222.16
957.28
241.33
709.42
Continued
284
hsa-miR-299
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-301
241.26
354.73
737.55
2717.90
133.28
1119.70
hsa-miR-302a
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-302a*
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-302b
63.42
17.29
34.34
27.70
63.59
114.87
hsa-miR-302c
32.21
0.00
0.00
0.00
0.00
0.00
hsa-miR-302c*
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-302d
0.00
0.00
14.45
2.13
0.00
0.00
hsa-miR-320
534.90
1229.68
2515.21
1362.62
839.31
2033.67
hsa-miR-321
9062.26
6436.94
6428.65
9100.69
5068.75
9831.49
hsa-miR-323
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-324-3p
258.23
277.85
444.47
2308.98
403.91
1186.91
hsa-miR-324-5p
41.59
109.83
146.97
950.35
44.15
883.63
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-325
hsa-miR-326
0.00
5.28
2.91
2.79
0.00
4.13
hsa-miR-328
16.30
103.22
186.85
491.61
37.99
698.99
hsa-miR-330
31.56
14.81
21.16
55.82
6.94
38.54
hsa-miR-331
1611.60
1616.46
940.25
4585.69
1808.18
3113.31
hsa-miR-335
119.61
56.60
0.00
0.00
20.73
15.39
hsa-miR-337
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-338
0.00
0.00
0.00
0.00
0.00
15.66
hsa-miR-339
110.36
77.48
70.42
43.05
3.57
112.03
hsa-miR-340
0.00
165.74
217.15
576.43
0.00
219.29
hsa-miR-342
1705.93
2946.96
1070.36
14531.22
690.51
1784.16
hsa-miR-345
103.55
1845.85
243.35
4617.01
31.42
714.86
hsa-miR-346
10.00
1.64
5.89
32.55
22.12
78.19
hsa-miR-361
19.35
357.66
78.53
443.88
6.25
330.99
hsa-miR-367
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-368
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-369
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-370
104.46
60.29
111.53
82.01
90.94
116.97
hsa-miR-371
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-372
122.99
100.71
118.58
110.00
93.90
232.23
hsa-miR-373
0.00
0.00
23.52
0.00
0.00
0.00
hsa-miR-374
1902.86
1720.37
1788.40
3138.22
375.59
4437.92
hsa-miR-375
0.00
0.00
25.16
24.20
0.00
467.00
hsa-miR-376a
0.00
0.00
11.92
0.00
0.00
7.46
hsa-miR-377
1.54
1.79
0.00
3.59
0.00
2.20
hsa-miR-378
117.34
213.12
325.88
74.62
28.10
241.53
hsa-miR-379
4.13
2.17
0.00
2.35
2.09
0.00
hsa-miR-380-3P
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-380-5p
0.00
0.00
0.00
0.00
0.00
0.00
Continued
285
hsa-miR-381
0.00
0.00
0.00
0.00
5.96
0.00
hsa-miR-382
0.00
0.00
7.66
0.00
3.48
0.00
hsa-miR-383
2.98
0.00
0.00
0.00
10.12
0.00
hsa-miR-384
0.00
0.00
0.00
0.00
0.00
0.00
hsa-miR-422b
103.70
144.22
194.45
40.90
26.31
184.74
hsa-miR-423
444.01
455.72
504.97
526.64
305.72
581.65
hsa-miR-425
224.34
564.43
265.32
504.65
10.67
179.91
let-7f(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
miR-129-5p(mmu)
0.00
22.19
0.00
0.00
6.32
0.00
miR-137(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
miR-224(mmu)
0.00
0.00
205.80
0.00
0.00
0.00
miR-424(mmu)
0.00
0.00
0.00
0.00
0.00
0.00
286
miRNA
let-7a-1*
Adipose
Bladder
Brain
Cervix
Colon
Heart
Lung
Liver
0.75
0.69
2.36
3.41
0.63
0.79
3.08
0.82
59.50
77.59
382.34
782.09
94.74
133.12
63.41
12.46
let-7a-3*
7.20
3.69
30.73
24.01
4.68
2.03
22.04
2.19
let-7b*
4.40
3.75
23.77
44.83
7.94
2.61
32.00
3.67
let-7c*
0.00
0.00
10135.72
0.71
0.00
0.00
0.00
0.00
let-7d*
31.17
31.52
52.17
156.60
81.39
28.07
222.35
46.10
let-7e*
2.04
1.17
3.40
5.98
0.89
0.90
4.96
1.26
let-7f-1*
0.00
0.00
0.00
0.00
0.00
0.66
0.00
0.00
let-7f-2*
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
let-7g*
0.00
0.00
1.73
3.05
0.00
6.58
2.86
1.34
let-7i*
0.00
0.00
3.36
22.06
1.85
14.82
2.80
0.00
let-7a-2-P
MIR-001-1
13.47
27.32
0.00
69.07
15.66
593.06
2.30
0.00
MIR-001-2
110.26
70.26
104.13
291.79
100.88
495.91
20.59
0.00
MIR-007-1
20.69
25.44
54.73
37.62
16.10
21.60
50.42
4.98
MIR-007-2
0.60
1.43
4.32
0.00
0.00
2.15
0.00
0.00
1.13
MIR-007-3
8.88
8.30
3159.42
12.83
114.50
5.82
0.00
MIR-009-1-P
25.62
19.76
64.32
37.55
121.07
21.16
4.05
9.72
MIR-009-2-P
19.21
40.38
4016.2
89.78
43.60
8.29
1.40
0.00
MIR-009-3-P
0.00
0.00
26.16
0.00
0.00
3.19
0.00
0.00
MIR-010a,b
4.66
7.73
0.00
32.78
21.20
0.00
12.46
0.00
MIR-015a
13.92
13.92
12.61
60.67
5.19
24.07
43.24
0.00
MIR-015b
31.84
29.94
66.48
66.43
22.14
43.33
169.60
17.31
MIR-016-1
44.78
10.32
32.91
103.59
58.68
62.70
82.10
9.60
MIR-016-2
1.62
0.78
30.46
34.06
20.79
6.13
13.21
0.00
MIR-017-P
34.40
28.13
4.51
127.28
37.37
28.99
60.31
17.94
463.78
527.88
507.12
1213.8
1356.1
1089.7
598.61
468.3
MIR-019a-P
0.99
0.00
1.78
10.99
9.10
6.21
0.90
3.75
MIR-019b-1-P
2.98
0.00
0.00
2.82
14.65
0.00
2.25
0.00
MIR-019b-2-P
0.00
0.00
1.93
0.00
0.00
0.00
0.00
0.00
MIR-018
MIR-020
0.00
1.49
0.00
4.66
0.00
0.00
3.07
0.00
MIR-021
726.86
4845.49
193.11
4116.8
6496.0
5296.2
2805.1
570.9
15.39
MIR-022-P
28.68
1.61
49.54
208.95
77.59
64.91
36.34
MIR-023a
95.83
16.55
29.70
1041.6
187.68
88.61
90.32
20.74
MIR-023b-P
98.35
54.73
167.75
804.29
108.67
150.83
133.93
38.30
MIR-024-1,2
1164.61
1651.96
2069.82
10869.64
3552.47
3825.21
1994.89
1121.87
Continued
Table C.3. microRNA precursor expression in 22 different normal human tissues

and cells. All data was generated using real-time PCR and SYBR green detection
except (*) which was assayed using TaqMan probes. Data is presented as copies of
microRNA per cell. (P) Real-time PCR was performed on the microRNA primary
precursor.
287
MIR-025
13.12
6.58
56.07
59.11
63.11
45.35
32.25
MIR-026a-1,2
0.00
10.06
58.38
52.45
4.14
16.27
15.28
0.00
MIR-026b
5.44
1.98
4.70
7.50
2.85
11.80
2.84
2.09
MIR-027a, b
6.17
37.91
8.20
407.18
30.76
40.89
60.72
10.59
MIR-028
8.95
7.21
0.00
30.84
8.61
6.65
5.48
0.00
30.74
11.75
14.38
95.23
12.30
17.92
17.48
0.00
0.00
MIR-029a,c
MIR-029b-1,2
27.79
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-030a,e
46.08
18.20
34.92
53.74
1.86
52.90
90.60
5.34
MIR-030b
90.44
28.99
72.64
162.46
81.66
215.04
77.58
53.26
MIR-030c-1,2
20.46
23.57
8.63
118.63
62.55
104.76
44.04
0.00
MIR-030d
93.16
13.61
15.84
47.13
23.93
63.52
73.58
0.00
MIR-031-P
16.66
18.93
61.40
63.24
24.67
29.96
5.29
1.82
MIR-032
49.61
12.65
36.33
29.74
34.87
13.77
34.25
5.14
0.00
MIR-033
3.72
0.00
0.00
3.30
4.68
0.00
3.49
MIR-034a
32.17
32.73
16.21
76.58
23.81
31.50
30.02
1.65
MIR-034b
1.66
0.00
23.86
5.67
4.13
9.75
1.96
0.00
MIR-092-1
0.00
0.00
7.31
33.61
0.00
10.08
5.28
2.05
MIR-092-2-P
0.00
0.00
7.84
0.00
3.28
0.00
0.00
0.00
MIR-093
9.89
27.13
22.54
25.63
47.26
26.85
9.15
16.41
MIR-095
11.46
0.00
41.77
10.74
6.99
10.67
0.78
0.00
MIR-096-P
27.63
14.29
84.64
26.31
44.66
65.84
4.95
14.22
1898.10
1058.63
1895.2
3204.8
1773.8
2686.1
833.86
543.0
MIR-098
MIR-099a
0.00
2.67
1.66
2.93
8.56
3.13
4.05
0.00
MIR-099b
181.14
140.46
206.02
572.16
252.30
363.67
181.13
22.74
69.39
454.57
959.06
1267.8
227.85
389.88
264.97
51.60
MIR-100
MIR-101-1
1.64
0.00
1.97
4.08
10.76
0.00
1.63
0.00
MIR-101-2
14.65
13.80
30.85
76.89
18.87
19.25
17.05
64.83
MIR-103-1,2
348.90
182.41
1059.69
1036.13
966.82
1007.07
437.91
245.06
MIR-105-1,2
1.06
1.64
1.00
0.87
5.34
12.00
1.79
0.00
MIR-106a-P
0.00
0.00
0.00
0.00
0.00
4.16
0.00
0.00
82.19
251.39
199.94
382.05
265.91
233.14
150.92
57.76
MIR-107
576.18
678.21
1464.2
1629.8
1841.9
2065.3
713.31
517.7
MIR-108
38.28
33.63
41.75
64.64
19.32
21.85
11.82
10.00
MIR-122a
0.00
0.00
2.96
0.00
0.00
0.00
0.00
0.67
MIR-124a-1,2,3
0.00
6.46
10029.82
18.13
6.02
3.72
4.38
9.11
MIR-125a
55.77
154.82
176.16
286.06
68.36
152.02
70.14
9.10
MIR-125b-1
42.62
131.17
348.57
556.36
58.62
128.66
30.18
1.57
MIR-125b-2
0.00
12.74
34.74
57.96
1.72
12.35
7.60
11.94
MIR-126
1009.52
675.68
265.20
915.89
290.71
903.77
898.90
192.6
MIR-127
77.52
64.04
118.27
144.14
118.64
130.83
28.18
51.12
MIR-128a
45.31
21.71
790.52
183.16
80.10
77.42
24.54
1.29
MIR-106b
Continued
288
MIR-128b
1.02
1.04
31.98
3.08
0.93
0.97
1.50
4.94
MIR-129-2
1.30
14.76
46.20
8.57
4.96
2.81
0.83
3.43
13.43
4.44
47.72
30.76
24.73
5.87
4.44
0.00
MIR-130a-P
MIR-130b
3.18
13.19
15.82
0.59
0.85
13.76
0.00
0.00
MIR-132
10.97
5.28
26.13
40.88
10.28
12.09
16.46
17.87
MIR-133a-1,2
12.25
19.25
55.32
34.69
24.94
158.24
0.00
0.00
MIR-133b
0.65
0.70
1.99
1.39
0.87
3.15
0.00
4.90
MIR-134
4.62
2.18
74.35
53.30
5.84
2.74
6.89
0.00
MIR-135a-1,2
39.70
15.12
64.95
47.51
65.65
1.11
13.94
0.00
MIR-135b-P
0.00
16.11
0.00
3.41
14.18
9.95
0.00
6.41
MIR-136
0.00
8.89
28.78
125.03
11.29
0.00
6.21
1.88
MIR-137*
0.00
0.00
16.33
1.48
0.00
0.00
0.00
0.00
MIR-138-1,2
0.00
0.00
18.71
0.00
0.00
0.00
0.00
0.00
MIR-139
33.48
25.96
9.37
43.23
43.28
40.62
18.43
10.91
MIR-140
1.87
0.00
1.21
1.38
1.84
0.00
1.11
0.00
MIR-141
7.97
3.16
0.00
4.18
15.83
0.00
12.38
2.15
MIR-142-P
33.77
7.39
3.82
30.93
41.94
3.41
22.62
0.00
MIR-143
104.91
277.38
8.82
628.55
161.54
62.42
42.98
2.63
MIR-144
10.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-145-P
525.56
268.21
53.67
3619.5
489.22
178.98
29.97
8.57
MIR-146
0.00
0.00
0.00
0.00
2.41
0.00
0.00
0.00
MIR-147
13.18
13.15
45.86
0.00
11.89
12.60
21.00
11.62
0.00
MIR-148a-P
2.01
0.00
0.00
0.00
0.00
1.09
0.86
MIR-148b-P
17.41
0.00
22.42
53.65
16.64
14.64
6.68
5.47
MIR-149
16.82
23.54
335.85
45.16
7.03
43.43
9.67
0.87
MIR-150
4.22
4.25
4.55
4.30
7.61
4.20
12.49
0.00
MIR-151
7.01
1.01
3.96
3.03
6.84
5.01
2.87
1.24
MIR-152
51.53
31.46
46.43
109.07
17.90
26.29
14.48
18.13
MIR-153-1,2
11.74
1.43
43.73
2.30
5.97
2.73
4.57
0.00
MIR-154
1.06
0.00
36.34
28.47
13.59
12.81
7.73
12.24
MIR-155
184.38
32.00
31.77
54.83
32.05
76.53
72.47
16.20
23.73
33.72
138.14
80.11
74.92
317.05
29.04
9.16
0.00
MIR-181a,c
MIR-181b-1,2
0.87
0.00
2.67
2.08
0.00
0.00
0.00
MIR-182
12.15
16.80
22.60
28.79
11.59
24.78
11.73
8.38
MIR-183
2.41
0.00
0.00
3.81
3.27
4.47
3.85
0.00
MIR-184
7.19
1.57
97.01
7.60
8.61
7.59
1.67
0.00
MIR-185
52.08
36.10
38.94
40.53
35.03
35.28
7.29
20.37
124.97
104.79
346.03
373.86
177.41
107.09
20.83
42.75
MIR-187
0.00
0.00
0.00
1.26
0.00
0.00
1.81
0.00
MIR-188b-P
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-190
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-186-P
Continued
289
MIR-191
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-192
1.32
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-193
31.16
21.52
0.00
23.81
10.55
48.89
5.36
11.17
0.00
MIR-194-1,2
0.00
0.00
0.00
3.62
0.00
0.00
0.00
0.91
MIR-195-P
26.84
0.88
32.63
34.48
13.20
12.65
3.63
0.00
MIR-196a-1
0.00
0.00
0.00
0.00
40.11
0.00
0.00
0.00
0.00
MIR-196a-2
5.99
1.42
2.61
13.71
5.15
0.00
0.00
1575.64
3263.01
0.00
13804.43
2520.04
6.07
29.39
0.00
MIR-197
22.00
9.81
67.67
95.68
83.04
37.20
12.42
16.33
MIR-198
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-199a-1-P
3.47
1.92
0.84
1.55
8.00
5.37
2.20
2.01
MIR-199a-2-P
12.45
5.14
71.30
124.54
12.83
20.45
6.26
14.25
MIR-199b-P
21.25
40.38
28.09
104.56
23.94
26.69
6.34
0.00
MIR-200a-P
60.06
MIR-196b
153.44
152.68
589.43
268.50
405.50
200.99
57.85
MIR-200b
0.00
9.55
1.77
31.39
72.70
0.00
6.09
0.00
MIR-200c
0.00
1.37
1.18
0.00
9.95
0.00
0.00
0.00
MIR-203
0.00
0.00
0.00
17.14
0.00
0.00
0.00
0.00
MIR-204
8.32
6.78
10.97
3.68
16.56
7.39
0.00
0.00
MIR-205
33.37
23.61
0.00
65.63
0.00
10.34
6.31
0.00
MIR-206
16.69
16.52
0.00
17.98
0.00
0.00
0.00
0.00
MIR-208
5.87
2.44
21.67
0.00
0.00
2256.4
0.00
1.73
MIR-210
112.55
174.54
41.88
80.49
9.98
122.63
73.45
20.63
MIR-211
52.17
10.25
23.82
54.99
28.07
31.18
12.56
12.73
MIR-212
186.76
202.37
135.24
717.92
202.71
152.57
80.99
28.90
MIR-213
30.36
13.32
12.71
17.02
16.14
0.00
12.50
2.18
0.00
MIR-214
9.77
14.66
39.65
9.35
8.80
5.03
4.74
MIR-215
18.57
0.00
14.16
40.69
28.00
24.88
8.34
6.06
MIR-216
0.00
0.00
5.23
1.04
1.04
0.00
0.00
0.00
MIR-217
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-218-1,2
94.62
238.06
113.61
991.28
70.39
103.03
166.77
2.08
MIR-219-1,2
0.00
9.60
3.57
0.94
14.92
0.00
3.71
0.00
MIR-220
1.58
8.57
519.70
3.87
1.78
2.25
0.00
0.00
MIR-221
35.50
53.29
27.42
241.34
53.48
25.43
45.50
0.00
MIR-222-P
13.61
13.26
5.65
130.65
23.59
0.00
18.78
18.17
MIR-223
3.16
0.00
5.09
4.41
1.96
3.21
5.32
0.00
MIR-224
18.79
20.36
11.86
117.02
37.94
36.23
10.37
0.00
MIR-296
0.00
2.44
11.84
12.02
0.00
7.84
0.00
0.00
18.74
9.35
7.73
10.49
9.31
0.00
4.43
13.83
MIR-301
0.00
0.00
16.34
11.01
23.07
16.81
13.88
18.67
MIR-302
6.85
6.46
19.22
0.00
19.09
0.00
3.55
0.00
20.25
8.75
13.74
15.59
15.99
13.30
5.78
0.00
MIR-299-P
MIR-302b
Continued
290
MIR-302c
0.00
0.00
0.69
2.33
1.69
0.00
0.59
0.00
MIR-302d
1.30
0.00
0.00
0.00
1.05
0.00
0.00
0.00
MIR-320
4.22
8.04
3.34
11.35
7.75
9.22
10.76
3.61
MIR-321
2026.23
8509.22
10664.52
6486.85
2916.11
11548.94
862.79
3178.97
MIR-323
8.53
5.45
95.13
38.68
10.59
12.65
14.09
2.19
43.01
45.07
37.01
61.30
15.46
77.17
22.75
6.68
3.49
MIR-324-P
MIR-325
444.34
5.85
202.97
8.87
2.24
9.07
3.11
MIR-326-P
4.44
2.57
22.99
6.92
5.23
1.47
7.59
2.31
MIR-328
0.92
2.71
7.70
2.68
2.01
1.69
0.00
1.59
0.00
MIR-330
0.00
0.00
55.77
1.29
0.00
3.59
5.94
27.83
53.55
30.79
132.00
18.73
21.44
42.89
6.38
MIR-335
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-337
0.00
0.00
3.39
15.66
10.99
0.00
0.00
0.00
MIR-331-P
MIR-338
0.00
0.00
50.94
0.00
3.03
6.46
7.40
12.68
MIR-339
347.06
414.19
800.47
597.76
329.61
584.73
181.97
123.1
MIR-340
284.94
307.41
1266.6
438.59
344.28
300.20
158.72
40.21
MIR-342
12.96
14.62
3.99
6.29
43.73
14.89
11.60
19.13
MIR-345
7.98
17.59
23.62
24.49
13.28
17.78
6.65
1.83
MIR-346
81.49
72.61
108.90
84.95
99.27
102.51
37.66
76.24
MIR-361
3.70
1.97
11.80
7.27
2.45
1.40
11.50
2.31
MIR-367
1.74
1.12
1.00
4.80
2.01
0.00
0.86
0.00
MIR-368
1.69
0.00
28.96
20.45
6.05
6.10
0.00
2.21
0.00
MIR-369
0.00
0.00
12.16
9.51
0.00
0.00
0.00
MIR-370
36.15
58.57
491.75
506.66
64.94
23.06
46.88
6.25
MIR-371
0.00
6.08
11.60
0.00
1.81
1.51
0.00
0.00
MIR-372
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-373
0.00
0.00
0.00
0.00
0.00
0.00
0.86
0.00
MIR-374-P
0.77
0.00
1.07
0.00
0.00
0.00
0.00
0.00
MIR-375
3.00
0.00
2.22
9.49
37.45
0.00
10.75
0.00
MIR-376
0.00
3.08
140.06
109.45
23.68
10.45
0.00
0.00
MIR-377
0.00
0.00
11.68
18.39
7.22
0.00
0.00
0.00
MIR-378
0.90
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-379
31.52
40.21
234.52
291.68
52.29
55.40
23.38
6.76
MIR-380
23.30
0.00
183.03
88.59
39.45
22.94
7.63
0.00
MIR-381
0.00
0.89
0.00
0.00
0.00
0.00
0.00
0.00
MIR-382
5.51
8.48
13.29
32.67
4.18
3.65
8.76
0.00
MIR-383
1.40
0.00
14.22
2.56
1.60
0.00
0.00
0.00
MIR-384
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-422b
0.63
0.86
2.74
0.00
0.80
0.00
0.00
MIR-423
10.68
10.89
18.99
16.24
10.71
12.92
5.44
0.00
MIR-424
0.00
10.88
0.00
99.02
2.91
0.00
0.00
0.00
Continued
291
MIR-425
miRNA
let-7a-1*
let-7a-2-P
let-7a-3*
18.94
Kidney
0.00
19.24
Esophagus
13.63
Skeletal
Muscle
2.66
Ovary
24.37
Pancreas
9.42
Placenta
0.00
Prostate
2.67
0.67
0.00
2.80
0.00
4.62
0.99
80.30
27.18
6.55
540.35
5.83
1314.62
62.17
14.78
8.55
3.97
1.68
46.81
2.17
24.25
let-7b*
16.56
5.99
6.16
88.87
5.85
20.91
8.05
let-7c*
0.00
0.00
0.00
0.77
0.00
0.00
0.00
let-7d*
175.14
30.88
97.64
208.96
20.97
83.62
24.85
let-7e*
4.38
1.85
0.00
8.44
0.75
5.22
1.63
let-7f-1*
0.00
0.00
0.00
3.54
0.88
6.73
2.68
let-7f-2*
0.00
0.00
0.00
0.00
0.00
0.00
0.00
let-7g*
1.64
0.00
2.31
24.85
6.30
90.73
9.94
let-7i*
2.00
1.64
11.79
58.97
16.98
43.95
18.59
MIR-001-1
0.00
45.93
789.78
21.24
0.00
0.00
45.50
MIR-001-2
26.67
97.04
963.41
121.68
26.64
90.52
144.03
MIR-007-1
20.21
15.14
2.83
37.16
9.66
27.09
15.61
MIR-007-2
0.00
0.00
0.00
0.00
0.71
0.00
0.00
MIR-007-3
6.23
8.57
0.00
28.99
931.86
0.00
0.00
MIR-009-1-P
29.37
13.90
13.74
24.83
7.53
17.52
38.68
MIR-009-2-P
63.01
33.59
4.70
111.30
34.36
45.60
45.27
MIR-009-3-P
MIR-010a,b
0.00
0.00
0.00
0.00
0.00
0.00
0.00
111.32
9.65
0.00
56.68
12.95
22.86
14.37
MIR-015a
15.82
6.11
10.72
38.63
0.00
135.08
5.37
MIR-015b
38.97
97.67
30.79
59.17
11.55
171.58
68.60
MIR-016-1
85.94
37.83
59.40
122.51
44.15
207.44
576.76
MIR-016-2
1.54
5.30
1.94
11.34
2.58
1.01
1.90
MIR-017-P
36.86
23.12
12.74
156.99
16.25
373.29
112.46
363.43
MIR-018
1424.95
542.80
455.34
1771.70
807.40
1309.32
MIR-019a-P
7.23
0.98
0.00
12.87
0.85
11.20
0.00
MIR-019b-1-P
7.59
0.00
0.00
2.08
0.00
23.18
5.09
MIR-019b-2-P
3.96
0.00
0.00
0.00
0.00
14.00
0.00
MIR-020
0.00
0.00
0.00
1.96
0.00
4.28
1.73
MIR-021
6882.19
5295.46
939.05
1427.79
2624.10
1657.19
1548.93
MIR-022-P
39.80
9.22
21.76
366.13
0.00
180.77
2.75
MIR-023a
48.60
44.38
26.09
382.38
10.42
576.04
52.50
MIR-023b-P
119.19
55.78
68.90
887.18
33.19
389.65
80.58
MIR-024-1,2
3574.82
3505.26
4960.45
3245.22
7158.98
2515.70
8900.50
MIR-025
38.56
12.37
55.15
98.13
8.26
120.23
48.30
MIR-026a-1,2
17.93
1.42
0.00
13.17
22.71
18.64
19.16
Continued
292
MIR-026b
10.16
6.82
4.53
3.36
4.16
14.54
4.06
MIR-027a, b
28.23
38.85
32.60
159.45
0.00
302.83
43.74
1.62
5.92
7.65
6.24
0.00
9.87
0.00
28.40
3.33
2.36
71.31
5.48
75.32
8.71
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-030a,e
117.16
8.78
8.84
49.69
7.42
499.41
36.98
MIR-030b
14.15
MIR-028
MIR-029a,c
MIR-029b-1,2
259.04
29.58
56.53
190.04
56.20
1130.36
MIR-030c-1,2
75.33
24.75
21.56
226.18
16.41
74.84
25.15
MIR-030d
79.50
7.22
0.00
77.36
0.00
843.44
25.51
MIR-031-P
15.21
14.42
4.15
17.36
6.93
24.02
17.28
MIR-032
41.50
16.68
38.30
108.15
21.98
40.95
18.73
MIR-033
0.00
0.00
0.00
0.00
0.00
3.48
0.00
MIR-034a
23.68
14.43
18.64
195.36
25.33
40.06
55.96
MIR-034b
0.00
1.57
0.00
0.00
0.00
35.42
0.00
MIR-092-1
13.07
0.00
0.00
7.63
5.88
25.61
0.00
MIR-092-2-P
17.74
0.00
0.00
0.00
0.00
13.39
0.00
MIR-093
40.09
10.70
0.00
34.32
14.57
28.86
13.17
MIR-095
2.95
10.85
14.85
2.88
0.00
0.00
0.00
49.49
37.35
50.85
26.64
19.61
16.88
14.93
731.19
MIR-096-P
MIR-098
3333.33
742.35
1343.40
5109.21
741.84
849.31
MIR-099a
3.03
1.20
0.00
7.02
1.99
4.86
1.59
MIR-099b
421.87
147.96
77.07
715.83
156.68
751.92
177.90
MIR-100
451.08
844.01
350.45
93.85
2389.21
70.90
2932.21
MIR-101-1
1.41
0.00
0.00
0.00
0.00
0.00
0.00
MIR-101-2
43.12
24.34
57.77
60.48
55.53
14.65
16.67
MIR-103-1,2
1304.78
746.74
790.22
1374.23
254.78
858.16
623.04
MIR-105-1,2
1.52
2.68
1.21
10.67
0.00
1.32
6.03
MIR-106a-P
1.11
0.00
0.00
0.00
0.00
1.05
0.00
291.23
218.75
203.83
625.82
103.98
266.55
187.79
MIR-107
2183.38
1228.07
1078.47
2007.52
376.09
1462.49
763.88
MIR-108
11.87
11.98
7.60
21.02
6.99
47.12
28.87
MIR-122a
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-106b
MIR-124a-1,2,3
71.88
8.38
5.50
11.31
0.00
0.00
35.51
MIR-125a
352.13
79.37
34.63
467.62
48.17
469.15
141.71
MIR-125b-1
102.23
47.98
26.20
702.85
29.93
148.68
67.67
MIR-125b-2
16.23
12.02
0.00
60.55
0.00
0.00
4.15
MIR-126
758.40
466.62
249.11
573.94
171.26
4231.07
429.80
MIR-127
78.63
111.59
49.85
52.50
46.12
157.32
46.62
MIR-128a
98.57
29.06
19.12
87.45
45.76
67.14
33.08
MIR-128b
0.00
16.50
1.85
5.13
0.00
12.65
1.11
MIR-129-2
10.39
8.45
0.00
4.80
7.84
5.67
2.64
Continued
293
MIR-130a-P
1.80
6.01
202.06
37.35
14.08
22.04
9.98
MIR-130b
1.86
1.10
1279.11
0.00
14.15
0.72
0.79
MIR-132
4.01
16.34
0.00
18.33
11.16
8.49
21.54
MIR-133a-1,2
17.13
11.08
0.00
13.69
19.75
34.01
20.07
MIR-133b
12.90
7.00
44.97
0.00
0.00
0.00
22.65
3.34
3.76
20.43
66.74
19.88
116.51
4.08
MIR-135a-1,2
17.58
10.94
14.56
630.15
34.48
21.64
26.10
MIR-135b-P
40.19
0.00
397.07
6.65
0.00
39.79
0.00
MIR-136
13.82
14.83
36.01
44.08
23.67
371.97
4.95
MIR-137*
0.00
0.00
0.00
0.00
0.00
1.55
0.00
MIR-138-1,2
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-139
33.40
56.28
35.44
14.73
33.24
28.84
22.33
MIR-140
1.53
0.00
0.00
0.00
0.00
0.00
0.00
MIR-141
13.21
13.94
1.52
0.00
28.03
20.57
4.51
1.36
1.27
13.71
22.22
10.31
50.83
0.00
55.43
349.42
5.30
190.18
6.14
65.88
121.08
MIR-134
MIR-142-P
MIR-143
MIR-144
0.00
0.00
0.00
0.00
0.00
0.00
0.00
85.34
256.59
5.41
1441.42
11.90
294.34
99.01
MIR-146
0.00
1.45
0.00
1.67
0.00
1.02
0.00
MIR-147
12.14
MIR-145-P
12.85
1.11
9.70
7.87
0.00
18.02
MIR-148a-P
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-148b-P
25.24
2.86
7.37
65.17
4.88
53.62
19.77
MIR-149
13.39
54.49
22.18
40.42
15.19
165.32
113.17
MIR-150
3.63
11.30
3.26
13.69
2.11
7.11
2.03
MIR-151
9.79
11.32
1.83
0.00
1.18
2.54
17.07
MIR-152
80.91
41.82
48.18
83.55
31.52
40.20
74.14
0.00
0.00
0.00
3.70
2.30
9.09
12.08
MIR-154
13.20
13.09
16.93
28.52
37.10
76.59
13.13
MIR-155
63.11
55.89
19.17
104.65
22.00
14.08
56.92
MIR-181a,c
93.44
18.78
20.71
87.43
31.46
64.89
60.17
0.00
0.00
0.00
0.00
0.00
1.61
0.00
MIR-182
139.76
96.09
548.20
11.64
123.54
22.62
71.04
MIR-183
5.37
3.40
1.71
0.00
4.84
0.00
6.09
MIR-184
4.88
5.39
0.91
3.35
15.65
2.59
3.38
MIR-185
42.62
42.39
19.25
25.51
47.78
32.75
18.16
MIR-153-1,2
MIR-181b-1,2
MIR-186-P
175.36
69.91
10.38
431.17
82.93
46.83
198.59
MIR-187
0.00
0.00
0.00
6.13
0.00
0.00
0.00
MIR-188b-P
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-190
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-191
0.78
0.00
0.00
1.17
0.00
0.00
0.00
MIR-192
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Continued
294
MIR-193
10.29
18.59
36.16
38.48
3.20
10.64
17.26
MIR-194-1,2
0.00
0.68
0.00
0.00
0.00
0.00
0.00
MIR-195-P
5.76
17.92
13.81
10.82
3.39
32.50
9.47
1.25
MIR-196a-1
0.92
1.66
0.00
0.00
0.00
0.00
MIR-196a-2
42.51
0.00
15.89
1.80
0.00
11.23
6.36
5283.01
27.73
4189.00
2575.39
0.00
569.86
2739.71
MIR-197
73.06
108.13
25.63
99.49
31.03
57.37
24.73
MIR-198
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-199a-1-P
4.80
5.25
4.28
5.74
2.72
5.06
1.40
MIR-199a-2-P
MIR-196b
25.76
1.59
4.81
348.71
0.00
57.92
3.87
MIR-199b-P
9.21
19.12
18.63
50.74
19.22
22.97
30.51
MIR-200a-P
142.29
167.16
145.97
222.65
74.56
178.21
148.86
MIR-200b
28.17
9.79
2.65
22.50
17.78
0.00
17.43
MIR-200c
0.00
0.00
0.00
0.00
0.00
0.00
5.02
MIR-203
0.78
90.01
0.00
0.00
0.00
0.00
0.00
MIR-204
65.37
9.14
0.00
8.30
5.15
0.00
0.00
MIR-205
7.10
147.52
0.00
0.00
0.00
28.07
107.83
MIR-206
14.04
113.46
2116.83
0.00
0.00
0.00
29.30
MIR-208
2.52
0.00
175.88
0.00
0.00
9.72
11.94
MIR-210
155.54
352.68
28.66
77.17
45.46
247.18
57.23
MIR-211
12.63
35.83
14.33
10.22
2.61
70.58
16.09
MIR-212
95.24
143.92
106.87
255.19
12.72
58.75
99.06
MIR-213
32.79
15.88
0.00
3.04
5.32
40.04
4.67
MIR-214
2.67
4.41
13.66
12.91
3.41
17.47
6.37
MIR-215
12.17
6.69
4.82
39.82
13.37
26.23
9.89
MIR-216
0.00
1.22
0.00
1.90
15.32
2.48
2.35
MIR-217
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-218-1,2
190.38
159.98
19.42
276.72
32.74
109.51
175.15
MIR-219-1,2
11.72
4.64
1.99
4.41
0.00
7.95
2.78
MIR-220
1.76
5.06
2.20
44.12
0.00
0.00
2.08
MIR-221
22.57
77.05
60.47
230.47
0.70
95.65
58.08
MIR-222-P
10.99
12.91
0.00
62.77
0.00
79.72
17.96
MIR-223
0.00
0.00
0.00
1.15
0.00
4.59
0.00
MIR-224
19.57
32.26
15.73
4.90
17.18
231.43
34.64
MIR-296
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-299-P
9.84
8.23
3.83
1.80
18.76
10.37
8.52
MIR-301
31.24
10.83
0.00
47.99
11.44
32.99
11.66
MIR-302
15.31
4.77
0.00
4.21
0.00
11.11
13.53
MIR-302b
25.22
7.94
0.00
27.78
0.00
4.70
16.07
MIR-302c
1.28
0.00
0.00
0.98
0.89
1.31
0.00
MIR-302d
0.00
1.28
0.00
0.00
0.00
0.00
0.00
Continued
295
MIR-320
6.42
9.47
3.11
21.59
6.26
23.10
8.75
MIR-321
4550.20
6835.94
1854.20
2747.62
1464.55
4763.74
24202.07
MIR-323
9.88
3.73
11.18
15.81
35.17
104.00
5.94
MIR-324-P
21.74
17.81
29.72
32.27
18.16
74.18
21.36
MIR-325
0.00
3.05
3.17
5.30
0.00
1.12
0.00
MIR-326-P
2.07
0.89
1.31
5.68
2.17
17.71
5.04
2.02
MIR-328
2.49
8.93
0.00
6.26
42.26
2.48
MIR-330
15.20
2.73
9.94
4.64
6.24
12.76
4.78
MIR-331-P
65.64
36.27
66.82
123.87
0.00
92.79
52.04
MIR-335
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-337
0.00
0.00
0.00
0.00
1.52
31.84
0.00
MIR-338
0.00
0.00
2.47
0.92
0.00
0.00
4.40
MIR-339
284.65
361.44
353.05
616.01
352.49
266.35
452.05
MIR-340
301.38
225.23
199.98
633.11
154.87
145.67
279.34
MIR-342
12.51
13.47
0.00
35.49
17.36
18.67
6.35
MIR-345
11.87
12.13
8.49
18.93
15.16
15.08
7.10
MIR-346
64.53
100.82
96.58
100.12
76.30
69.58
87.84
MIR-361
8.42
3.92
7.40
33.86
2.65
16.99
2.33
MIR-367
0.00
0.92
0.00
1.00
0.91
0.00
0.71
MIR-368
2.39
1.37
0.00
4.35
9.77
54.22
0.00
MIR-369
0.00
0.00
0.00
6.69
8.82
24.90
0.00
MIR-370
17.47
41.69
44.11
277.54
123.46
813.43
129.97
MIR-371
5.60
4.37
0.00
1.99
1.67
0.00
0.00
MIR-372
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-373
0.00
0.00
0.00
0.00
0.00
0.00
1.56
MIR-374-P
0.00
0.00
0.00
0.00
0.00
0.00
1.88
MIR-375
0.00
19.72
1.08
0.00
42.33
0.00
2.68
MIR-376
4.45
3.72
0.00
32.03
26.12
295.93
5.65
MIR-377
10.69
4.28
0.00
13.93
10.84
117.97
16.92
MIR-378
0.00
0.00
1.17
0.00
0.00
0.00
0.00
MIR-379
35.74
24.57
22.77
196.88
164.84
406.09
27.64
MIR-380
9.10
11.44
0.00
76.74
28.55
192.46
12.91
MIR-381
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-382
2.94
0.00
0.00
4.41
0.00
41.93
0.00
MIR-383
0.00
1.77
0.00
2.52
0.00
0.00
2.90
MIR-384
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-422b
0.00
1.38
0.00
0.00
1.48
0.00
0.00
MIR-423
27.54
10.66
3.43
6.96
1.83
13.64
7.97
MIR-424
15.37
0.00
30.63
55.54
0.00
44.63
0.00
MIR-425
9.72
15.61
7.35
14.28
8.43
13.93
0.00
Continued
296
miRNA
let-7a-1*
Spleen
Small
Intestine
Trachea
Thymus
Thyroid
Testes
Normal B
cell
3.76
2.02
4.22
1.03
1.54
1.98
5.58
let-7a-2-P
26.98
15.13
143.89
122.84
68.63
103.55
13.63
let-7a-3*
9.56
15.99
8.80
42.01
6.10
5.28
9.11
let-7b*
8.56
5.37
30.85
6.55
6.52
24.05
9.11
let-7c*
0.00
0.00
0.00
0.00
0.00
0.00
0.00
let-7d*
41.06
15.57
70.48
47.31
74.16
98.78
118.02
let-7e*
0.66
0.00
0.75
0.92
1.10
2.40
0.00
let-7f-1*
4.18
0.00
0.00
0.00
0.00
0.89
10.06
let-7f-2*
0.00
0.00
0.00
0.00
0.00
0.00
1.31
let-7g*
24.78
1.36
0.00
4.44
1.62
7.14
106.34
let-7i*
51.62
1.67
4.43
11.52
19.86
16.06
118.28
MIR-001-1
0.00
4.67
14.73
0.00
22.62
24.42
0.00
MIR-001-2
121.34
87.09
211.46
163.23
105.04
140.26
124.30
MIR-007-1
62.90
44.46
57.11
42.30
33.41
31.67
879.06
MIR-007-2
0.00
0.00
1.25
1.20
0.72
0.00
0.96
MIR-007-3
4.90
116.63
2.41
13.43
0.00
1071.3
10.99
MIR-009-1-P
32.98
17.31
38.14
12.39
20.91
33.38
68.53
MIR-009-2-P
32.40
61.84
46.46
44.75
11.89
57.90
68.33
0.00
MIR-009-3-P
0.00
0.00
0.00
0.00
0.00
0.00
MIR-010a,b
29.48
48.88
12.89
12.43
7.35
44.76
7.17
MIR-015a
187.92
28.53
82.41
17.29
11.00
19.21
576.46
MIR-015b
269.72
47.19
109.93
331.21
43.99
156.56
450.15
MIR-016-1
494.76
107.08
184.33
578.36
34.30
179.48
745.52
MIR-016-2
7.13
48.31
20.17
79.11
4.39
24.40
27.50
MIR-017-P
371.57
33.60
176.83
43.16
33.52
42.23
975.57
MIR-018
2315.1
1424.52
972.14
2457.57
408.89
1534.5
4032.20
MIR-019a-P
24.24
4.00
5.49
1.61
0.93
2.81
80.23
MIR-019b-1-P
19.08
0.00
3.84
0.00
4.52
10.31
77.38
MIR-019b-2-P
0.00
0.00
0.00
0.00
0.00
0.74
5.05
MIR-020
0.00
0.00
4.01
0.00
4.56
0.00
24.15
MIR-021
4264.4
5613.86
3144.56
1882.57
764.66
2173.4
709.24
MIR-022-P
174.22
95.63
267.69
18.31
5.42
24.09
736.45
MIR-023a
132.66
112.12
258.68
40.60
50.93
46.03
352.13
MIR-023b-P
189.68
72.98
317.74
20.83
36.97
47.51
51.81
MIR-024-1,2
2610.1
3192.14
4313.32
1471.94
1017.73
1579.6
894.92
MIR-025
114.28
58.79
64.42
91.20
35.45
74.46
170.41
20.65
28.55
36.90
19.21
10.46
33.74
2.61
MIR-026a-1,2
MIR-026b
MIR-027a, b
15.21
18.21
5.94
5.12
15.10
2.35
12.25
109.63
21.79
125.60
17.08
11.24
41.09
314.92
Continued
297
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-030a,e
1.29
9.70
7.20
3.25
0.00
1.28
29.30
77.39
16.40
56.27
32.23
8.54
44.52
178.18
0.00
0.00
0.00
0.00
0.00
0.00
0.00
44.61
51.39
10.42
52.14
27.41
28.25
22.19
MIR-030b
230.63
153.23
204.49
94.47
35.04
155.84
35.93
MIR-030c-1,2
295.80
92.35
76.67
54.82
0.00
40.00
220.32
MIR-030d
114.20
16.05
123.63
0.00
16.23
19.93
66.64
MIR-031-P
22.82
38.43
83.25
16.58
6.32
34.47
56.06
108.94
83.47
48.01
31.77
51.41
141.25
69.72
70.27
MIR-032
MIR-033
0.00
0.00
4.40
0.00
0.00
0.00
MIR-034a
26.79
24.42
73.96
48.13
21.61
128.14
6.50
MIR-034b
0.00
0.00
13.40
1.54
0.00
20.13
0.00
MIR-092-1
12.85
0.00
3.09
0.00
6.49
5.50
154.46
MIR-092-2-P
35.84
0.00
6.09
0.00
0.00
0.00
0.00
MIR-093
38.33
19.47
26.04
108.38
13.60
53.36
77.21
MIR-095
13.11
0.00
0.00
0.00
6.33
0.00
15.95
MIR-096-P
27.08
58.54
52.25
18.93
20.00
32.74
58.64
2988.6
1546.78
1759.25
1017.56
817.07
2528.8
1946.42
MIR-099a
2.15
4.33
4.78
2.56
0.62
9.21
0.00
MIR-099b
390.87
20.36
267.57
98.02
121.27
513.83
11.88
74.40
183.60
181.89
516.16
290.00
911.31
0.00
0.00
2.14
0.00
0.00
0.00
0.79
8.26
MIR-098
MIR-100
MIR-101-1
MIR-101-2
83.67
36.37
46.66
12.11
6.35
11.27
27.78
MIR-103-1,2
1736.8
1173.50
911.28
1543.24
388.24
2042.4
988.41
MIR-105-1,2
0.00
4.98
1.01
0.00
1.53
1.40
1.66
MIR-106a-P
0.00
0.84
0.00
0.00
0.00
0.00
0.00
MIR-106b
643.23
365.45
155.34
1217.84
112.89
505.86
374.02
MIR-107
2599.9
1795.67
1130.08
2155.88
595.79
3071.9
1141.60
MIR-108
24.67
22.08
29.42
21.71
23.79
24.28
0.00
MIR-122a
0.00
0.00
0.00
0.00
0.00
0.00
0.00
48.37
28.91
9.75
0.84
0.79
133.38
2.36
153.37
43.12
85.41
36.28
94.69
180.34
6.87
MIR-125b-1
34.04
77.81
98.61
65.13
95.08
252.57
10.26
MIR-125b-2
0.00
0.00
0.74
11.66
0.00
13.43
0.00
1919.2
398.62
356.44
391.31
323.62
341.22
20.25
MIR-124a-1,2,3
MIR-125a
MIR-126
MIR-127
58.85
32.27
81.48
13.06
78.19
46.30
170.98
MIR-128a
283.02
74.01
123.25
41.73
23.87
104.80
395.36
MIR-128b
1.72
0.60
1.90
350.06
5.73
2.24
3.68
MIR-129-2
14.43
7.05
14.11
9.73
29.07
19.58
46.35
MIR-130a-P
27.95
19.33
29.53
6.84
14.95
11.74
1.75
0.00
12.74
23.73
12.67
12.33
13.76
5.86
MIR-130b
Continued
298
MIR-132
14.41
25.06
33.16
10.68
17.16
30.97
24.00
MIR-133a-1,2
17.50
32.24
33.68
10.26
16.37
27.26
188.07
0.00
18.66
4.56
4.91
0.00
0.00
77.50
MIR-133b
MIR-134
61.16
9.98
21.90
5.95
2.86
53.70
96.61
MIR-135a-1,2
55.93
22.03
31.35
55.86
16.96
440.41
199.79
MIR-135b-P
18.35
31.83
85.99
13.60
32.54
13.72
24.45
MIR-136
38.94
17.52
15.16
1.90
0.00
113.15
11.63
MIR-137*
0.00
0.00
0.00
0.00
0.00
1.50
0.00
MIR-138-1,2
9.46
0.00
11.27
0.00
0.00
0.00
0.00
MIR-139
33.06
38.28
45.56
51.89
30.19
30.16
20.55
MIR-140
1.28
0.95
2.06
0.78
0.75
0.83
8.87
MIR-141
0.00
55.91
7.05
8.80
2.98
13.43
52.01
MIR-142-P
272.27
22.74
10.50
13.85
56.46
1.27
1067.31
MIR-143
111.53
86.95
74.34
14.06
14.13
74.32
2.21
MIR-144
0.00
12.48
0.00
0.00
0.00
0.00
13.24
542.91
53.48
557.61
19.20
13.76
146.10
2.31
MIR-145-P
MIR-146
0.98
0.00
2.60
0.00
0.00
0.00
0.00
MIR-147
21.87
11.06
20.47
3.33
11.25
1.28
14.02
MIR-148a-P
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-148b-P
4.96
117.13
32.24
73.32
0.00
0.00
29.58
MIR-149
7.60
14.08
35.69
181.88
4.92
115.10
5.76
MIR-150
69.00
12.71
9.17
104.88
1.28
11.57
86.41
MIR-151
23.13
15.29
5.20
1.19
4.71
14.31
21.16
MIR-152
51.36
27.49
41.70
16.87
53.88
238.29
14.63
8.37
4.55
9.29
2.09
0.00
21.57
16.16
MIR-153-1,2
MIR-154
14.18
13.78
13.18
12.76
0.00
24.64
0.00
MIR-155
571.36
76.52
155.47
387.04
16.45
98.76
162.46
MIR-181a,c
229.72
103.91
55.70
270.13
141.25
87.35
10.32
MIR-181b-1,2
1.90
0.00
1.04
5.51
0.00
11.20
0.00
MIR-182
0.00
61.16
108.60
139.94
8.52
709.30
16.64
MIR-183
2.56
1.64
2.64
1.80
5.40
11.11
0.00
MIR-184
4.20
4.00
2.67
2.02
1.40
54.33
8.18
MIR-185
22.82
46.63
33.84
17.61
29.95
43.50
74.87
465.19
192.78
407.65
39.92
37.49
284.16
515.35
MIR-187
0.00
0.00
0.00
0.00
0.00
0.00
2.50
MIR-188b-P
0.00
2.23
0.00
0.00
0.00
0.00
0.00
MIR-190
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-191
2.48
0.00
0.00
0.00
0.00
0.00
15.83
0.00
MIR-186-P
MIR-192
0.00
0.00
2.38
0.00
0.00
0.00
MIR-193
27.32
14.14
26.48
6.42
22.02
10.41
7.84
2.19
0.76
0.78
0.92
0.00
1.30
2.65
MIR-194-1,2
Continued
299
MIR-195-P
14.76
5.11
25.34
11.35
12.76
8.37
45.97
MIR-196a-1
0.00
0.58
0.00
1.95
0.00
3.02
7.77
MIR-196a-2
9.53
0.00
11.48
0.71
8.02
22.46
0.00
MIR-196b
59.01
18.36
72.26
2968.06
0.00
794.75
12.63
MIR-197
170.72
78.22
65.19
24.08
8.50
95.37
456.62
MIR-198
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-199a-1-P
13.40
4.19
8.59
13.66
3.46
5.24
51.66
MIR-199a-2-P
180.20
26.78
45.31
10.04
3.48
24.68
12.75
MIR-199b-P
26.09
3.93
30.15
13.55
11.06
40.21
44.58
MIR-200a-P
493.96
211.03
272.04
374.90
180.80
121.46
279.70
MIR-200b
1.84
56.75
76.41
8.36
15.43
0.00
6.50
MIR-200c
0.00
10.07
10.45
0.00
9.58
0.00
12.83
MIR-203
8.52
20.82
0.62
1.69
16.64
21.52
0.00
MIR-204
20.22
0.00
12.65
1.88
3.91
13.81
1.70
MIR-205
0.00
0.00
228.76
82.69
0.00
4.25
0.00
MIR-206
0.00
0.00
0.00
43.22
0.00
10.70
0.00
MIR-208
3.30
0.00
1.10
0.00
1.96
0.00
4.33
MIR-210
47.39
99.78
196.22
232.78
22.58
196.01
39.17
MIR-211
18.55
5.24
23.11
30.31
18.01
16.89
54.18
MIR-212
84.07
237.55
288.11
202.56
57.46
280.21
20.46
MIR-213
23.78
20.15
0.00
204.50
0.00
25.67
51.34
MIR-214
5.06
2.70
8.20
13.24
2.52
13.29
14.31
MIR-215
28.37
31.00
22.70
30.58
14.29
34.56
125.74
MIR-216
0.00
0.00
0.00
1.75
1.02
0.00
3.41
MIR-217
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-218-1,2
285.05
85.82
164.76
94.98
45.67
243.56
9.99
MIR-219-1,2
1.61
4.87
8.38
6.10
2.38
5.74
8.92
MIR-220
8.98
3.50
20.11
6.10
2.03
133.63
14.91
MIR-221
52.24
23.83
86.11
34.64
37.60
15.59
115.02
MIR-222-P
28.69
0.00
54.51
20.18
10.99
15.30
32.35
MIR-223
20.20
0.00
3.26
0.00
0.00
0.00
33.32
MIR-224
19.96
59.41
24.60
19.73
6.86
27.13
2.10
MIR-296
11.38
0.00
11.37
11.56
0.69
16.04
3.08
7.79
5.04
0.00
9.27
9.65
8.45
52.46
26.16
44.44
20.75
208.64
10.46
34.45
17.26
MIR-299-P
MIR-301
MIR-302
7.47
7.28
5.80
4.20
14.16
12.79
48.90
MIR-302b
15.67
10.36
10.31
21.27
11.14
10.35
28.78
MIR-302c
1.13
0.00
1.69
0.00
0.00
0.00
4.90
MIR-302d
0.00
0.00
0.00
0.00
0.00
1.62
0.00
MIR-320
9.24
32.28
8.39
10.97
9.42
30.28
5.26
MIR-321
3499.4
1971.63
2990.43
1050.89
1658.58
3033.7
577.39
Continued
300
MIR-323
19.49
5.51
8.25
10.28
3.34
42.48
6.52
MIR-324-P
29.07
68.12
53.56
21.95
51.69
52.45
184.79
0.00
10.03
6.66
47.78
7.18
2.35
0.00
MIR-325
MIR-326-P
22.11
8.00
9.19
1.19
0.00
1.37
0.00
MIR-328
6.81
4.06
8.08
2.19
0.62
7.32
18.43
MIR-330
20.40
16.26
7.47
20.44
6.31
4.95
6.41
MIR-331-P
53.83
53.65
9.67
135.36
21.64
103.69
12.88
MIR-335
0.00
10.09
0.00
0.00
0.00
0.00
2.57
MIR-337
0.00
0.00
0.00
7.00
0.00
4.73
0.00
MIR-338
8.69
10.85
1.26
0.00
1.30
0.00
0.00
MIR-339
412.13
324.99
315.69
238.68
269.71
348.19
405.80
MIR-340
482.21
276.93
445.17
154.46
100.26
488.53
174.58
MIR-342
101.21
38.81
20.73
178.57
0.00
9.98
54.56
MIR-345
7.58
13.57
35.72
10.19
13.19
4.70
38.72
MIR-346
89.10
68.15
103.79
77.76
77.72
60.60
123.37
MIR-361
3.91
5.06
12.24
8.23
6.52
5.91
43.85
MIR-367
3.42
1.07
0.97
1.49
2.16
1.09
6.72
MIR-368
2.79
0.00
1.73
5.49
0.00
14.60
0.00
MIR-369
7.58
0.00
0.00
7.95
2.06
3.57
6.25
MIR-370
198.22
55.97
39.50
23.50
12.07
323.43
0.00
MIR-371
7.05
0.00
0.00
0.00
0.00
11.53
8.21
MIR-372
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-373
14.31
0.00
1.50
0.00
1.25
0.00
2.38
MIR-374-P
0.00
0.00
0.95
0.00
0.86
0.65
2.74
MIR-375
0.00
31.53
17.04
0.00
1.30
34.96
0.00
MIR-376
33.75
0.00
8.22
10.55
5.26
36.88
6.08
MIR-377
8.01
0.00
15.80
0.00
0.00
10.59
0.00
MIR-378
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-379
147.88
25.66
69.91
51.20
0.00
276.33
0.00
MIR-380
43.97
11.61
39.43
30.73
0.00
36.36
0.00
MIR-381
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-382
13.42
0.00
2.88
0.00
0.00
8.88
6.15
MIR-383
0.00
0.00
1.80
2.49
1.52
1.69
1.10
MIR-384
0.00
0.00
0.00
0.00
0.00
0.00
0.00
MIR-422b
0.00
2.02
0.60
0.00
0.82
3.37
0.00
MIR-423
16.38
12.02
9.16
8.49
10.03
11.99
32.23
MIR-424
8.90
0.00
0.00
16.17
0.00
59.19
0.00
MIR-425
24.30
15.85
21.78
9.17
0.00
95.41
314.06
301
miRNA
Adipose
Bladder
Brain
Cervix
Colon
Heart
Lung
Liver
hsa-let-7a
hsa-let-7b
hsa-let-7c
hsa-let-7d
hsa-let-7e
mmu-let-7f
hsa-let-7g
hsa-let-7i
hsa-miR-001
hsa-miR-007
hsa-miR-009
hsa-miR-009*
hsa-miR-010a
hsa-miR-010b
hsa-miR-015a
hsa-miR-015b
hsa-miR-016
hsa-miR-017-3p
hsa-miR-017-5p
hsa-miR-018
hsa-miR-019a
hsa-miR-019b
hsa-miR-020
hsa-miR-021
hsa-miR-022
hsa-miR-023a
hsa-miR-023b
hsa-miR-023bN
hsa-miR-024
hsa-miR-025
hsa-miR-026aN
hsa-miR-026b
hsa-miR-026bN
hsa-miR-027a
hsa-miR-027b
hsa-miR-027bN
hsa-miR-028
hsa-miR-029a
hsa-miR-029b
hsa-miR-029c
hsa-miR-030a-3p
hsa-miR-030c
10613.6
53686.2
15171.5
463.0
177.4
0.0
14248.8
1833.3
9109.7
148.0
447.3
175.8
3456.7
1955.6
1469.0
7599.0
23401.5
75.3
907.5
59.6
4266.5
31714.8
39732.0
26741.7
2932.6
504.8
745.7
12333.9
120059.2
3006.7
129641.3
47027.8
35087.9
25238.1
3825.6
40027.2
5384.3
12067.4
155.8
3901.0
11168.8
54329.4
13363.8
58352.8
24047.5
1577.2
870.9
0.0
27606.9
3157.4
63472.5
358.4
1362.2
347.5
2843.5
2675.7
2809.8
13804.4
45700.8
249.2
2031.1
148.6
12744.0
85010.3
95865.6
64947.7
3534.5
969.0
8038.1
61401.4
415038.3
4079.1
252327.8
92977.3
68284.4
43315.8
27048.3
203039.0
18487.0
40038.3
912.4
13766.6
13205.0
96796.9
11789.5
41012.0
18520.0
1288.6
837.0
0.0
18884.3
1586.6
1325.4
6796.9
259711.7
59637.3
178.8
1.6
2106.9
4762.4
22086.4
103.2
1124.0
63.7
5282.6
33748.2
39963.5
4647.8
1178.6
124.3
3460.0
20958.3
87669.7
2281.9
142130.1
26848.6
20878.7
3762.6
6982.0
69936.8
1583.6
20879.1
704.7
5738.9
8839.6
71410.0
19063.4
98751.3
59273.5
1405.7
799.3
0.0
28180.2
3235.7
17524.0
258.3
5183.9
1009.5
7577.7
5923.1
1700.8
10478.2
33104.2
161.9
1920.1
88.5
11758.0
72824.1
67326.7
41063.0
2001.7
796.6
7083.1
52388.3
377404.3
4470.8
346478.5
92781.3
68247.7
39226.1
17730.8
163833.7
11186.5
46124.3
705.0
10038.4
8944.4
82185.6
7767.6
24511.0
4039.5
868.4
445.3
0.0
14598.9
1367.8
19352.9
1548.2
1958.8
467.1
9972.8
3506.6
1911.6
10013.2
23722.6
129.7
3358.2
263.0
18071.8
92091.2
92793.7
55004.4
1482.6
458.8
4230.6
26920.6
205009.3
2686.5
130102.6
37773.2
28734.4
25347.8
10084.8
82223.1
10903.8
19275.5
712.5
12226.1
5340.5
53298.5
8369.8
16205.2
9002.8
1002.0
387.1
0.0
12733.3
1307.6
329801.5
185.4
734.3
141.6
846.7
439.6
1290.5
7506.7
19664.9
57.9
1453.4
77.7
6834.0
38935.5
41833.1
14811.2
4687.4
720.5
6009.6
96699.1
406258.4
1822.5
126069.0
46607.2
31876.8
28601.6
16640.2
122599.9
6515.5
19710.3
682.7
11286.7
16837.6
182162.9
11502.0
46432.0
8229.3
1286.1
424.4
0.0
15906.0
1755.2
1615.6
251.4
287.3
53.7
4004.1
655.5
2697.0
16577.5
34670.9
71.1
1701.9
172.4
10696.8
64131.5
67678.4
72170.0
1309.6
562.6
3244.9
15497.5
229790.8
3062.5
142534.4
46037.5
34435.5
23923.0
5480.0
63230.6
4118.1
22755.2
768.8
10195.7
13483.9
66838.1
3382.1
11378.6
5076.9
269.1
98.8
0.0
5263.4
306.8
110.5
24.9
100.6
32.0
1117.6
84.6
592.9
3021.3
4660.6
33.1
1352.3
83.3
7416.0
43563.2
41971.9
10514.9
1090.6
65.3
2064.0
17540.4
68404.9
1185.3
35995.3
21980.7
15203.7
3136.4
3537.9
40280.4
2529.9
6667.7
235.2
2282.9
3227.8
35011.5
Continued
Table C.4. Mature microRNA expression in 22 different normal human tissues and
cells. All data was generated using real-time PCR and TaqMan microRNA assays
(Applied Biosystems). Data is presented as copies of microRNA per cell.
302
hsa-miR-030d
hsa-miR-030e-3p
hsa-miR-031
hsa-miR-032
hsa-miR-033
hsa-miR-034a
hsa-miR-034aN
hsa-miR-034b
hsa-miR-034b
hsa-miR-092N
hsa-miR-093
hsa-miR-095
hsa-miR-096
hsa-miR-098
hsa-miR-099a
hsa-miR-099b
hsa-miR-100
hsa-miR-101
hsa-miR-103
hsa-miR-105
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-108
hsa-miR-122a
hsa-miR-124a
hsa-miR-125a
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
hsa-miR-127
hsa-miR-128a
mmu-miR-129-2
hsa-miR-130a
hsa-miR-130aN
hsa-miR-130b
hsa-miR-132
hsa-miR-133a
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
mmu-miR-137
hsa-miR-139
hsa-miR-140
hsa-miR-141
hsa-miR-142-3p
8337.8
2010.1
2073.0
984.4
0.0
65.1
1677.3
5.7
0.0
19228.1
15068.3
1564.2
468517.2
613.1
15038.0
3098.7
10345.8
80.4
3061.9
641.3
45983.6
2435.1
5.4
0.0
1.5
0.0
50192.1
47325.8
217325.4
19034.3
1251.4
0.0
0.0
226.4
3544.7
18.2
2478.2
894.8
184.8
28.5
285.6
0.0
598.4
12329.2
140.5
3258.8
15915.5
4862.3
13163.9
2165.0
0.0
420.7
5991.5
13.6
15.8
32751.1
34559.4
1542.3
639331.1
923.3
51060.1
7570.8
32294.3
498.8
4921.9
1024.4
94030.2
7617.3
23.9
0.0
0.0
0.0
93609.2
109389.7
279589.6
32416.8
4901.2
0.0
3.6
2195.3
13294.9
82.4
9980.9
9360.7
448.8
1134.0
682.6
45.2
557.2
26575.0
993.3
18481.3
5934.4
3418.0
5489.7
1389.1
8.0
262.5
2872.9
31.2
39.0
25649.4
18272.4
6938.8
782408.6
2036.6
44826.2
9293.8
27832.9
81.7
9048.6
759.3
105822.5
2762.7
108.4
0.0
0.0
4859.2
90938.5
124707.2
91681.9
9665.8
24597.2
112.5
119.5
711.7
3116.4
78.1
48090.7
259.4
1803.0
4844.5
681.7
3798.6
1700.1
15428.3
7.6
3017.4
11902.9
4468.2
14518.5
1764.9
23.7
448.2
5505.9
72.6
26.3
35311.3
17943.1
1222.8
481715.0
870.1
87244.3
9091.4
50040.3
672.6
5119.5
1125.1
58176.8
5292.2
0.0
0.0
1.7
0.0
126526.5
212464.6
248430.4
33397.8
19442.5
8.5
0.0
5060.9
26032.9
123.2
7490.7
2386.2
1501.0
2949.6
2933.3
93.5
364.6
36322.4
1517.9
12062.2
8795.8
3215.0
24363.7
1608.9
0.0
114.5
1953.6
8.6
33.3
30903.8
27656.5
1460.5
389223.7
563.3
12820.7
3670.2
8073.8
251.4
3163.5
799.4
38763.9
6471.7
29.8
0.0
1.8
0.0
44090.2
27670.1
123410.2
15968.7
3838.5
0.0
0.0
1695.2
6835.0
119.1
7211.4
3162.2
295.9
764.9
1367.2
107.7
255.7
15781.1
5994.4
30539.7
19264.4
11594.2
257.6
1899.1
0.0
194.0
2572.7
0.0
9.9
24967.7
12782.2
2261.2
515124.3
642.2
22632.7
6916.7
13291.2
273.8
3186.7
546.9
66565.9
2673.9
51.3
0.0
0.0
0.0
66642.5
57528.3
278539.3
32268.2
3788.0
0.0
0.0
1792.5
6236.2
102.5
6003.6
84221.7
211.8
638.7
146.5
18.6
288.7
18077.5
0.0
6799.8
18899.8
2399.2
3025.4
2047.1
20.0
637.5
5635.5
785.5
829.7
23609.6
23722.9
1063.6
378240.7
708.8
16840.6
4356.8
11666.9
455.6
4475.3
656.3
59599.1
4415.2
9.7
0.0
0.0
0.0
59930.1
26785.3
445485.7
43612.7
2517.6
0.0
0.0
2534.8
13324.3
93.9
5579.4
242.3
216.4
657.1
5183.0
10.6
121.9
22344.8
3630.2
53195.0
4098.8
2989.7
155.3
1062.9
8.4
143.0
1573.6
0.0
0.0
21977.0
7257.9
272.2
390082.2
198.2
6074.1
568.6
4477.4
181.4
1760.1
564.2
67641.6
1362.8
34.0
0.0
4135.6
0.0
10285.3
11550.6
75287.0
8614.8
1124.4
0.0
0.0
607.2
2273.4
159.3
825.3
29.4
131.8
18.1
165.1
0.0
129.4
4913.8
33.1
7053.9
Continued
303
hsa-miR-142-5p
hsa-miR-143
hsa-miR-145
hsa-miR-146
hsa-miR-146b
hsa-miR-147
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-152
hsa-miR-152N
hsa-miR-153
hsa-miR-154
hsa-miR-154*
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-182
hsa-miR-183
hsa-miR-184
hsa-miR-186
hsa-miR-187
hsa-miR-188b
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196b
hsa-miR-197
hsa-miR-199a
hsa-miR-199a*
hsa-miR-199b
hsa-miR-199-s
hsa-miR-200a
hsa-miR-200b
hsa-miR-200bN
hsa-miR-200c
hsa-miR-200cN
hsa-miR-203
hsa-miR-204
hsa-miR-205
337.0
796.5
2395.0
14186.4
13234.0
1.4
1337.0
292.7
741.5
59978.4
10710.5
7113.8
7.0
0.0
0.0
2127.3
907.5
2516.9
67.1
3.6
47.8
12.2
6979.8
25.6
80.8
180.1
18254.3
875.1
884.6
199.0
49023.9
4767.5
313.3
4561.6
189.0
15167.1
201.6
810.2
853.0
1077.4
1847.4
15013.1
8158.9
9762.5
2878.6
4460.3
2397.5
34165.5
30948.1
14772.1
22446.6
2.9
5520.8
1520.6
2623.2
48226.4
16574.7
12652.8
17.9
0.0
0.0
3234.0
1008.2
2808.2
158.9
11.9
69.7
50.3
14940.1
1229.3
121.6
253.9
30003.3
3543.3
1500.6
932.0
92401.5
1834.0
2195.7
7734.1
331.5
28938.3
289.3
2325.5
3753.7
2117.7
5569.1
21230.7
12275.5
3695.6
7813.0
4632.7
513.7
411.2
991.1
6301.9
9139.1
3.5
518.6
1279.1
35807.8
10449.2
3196.3
2671.9
311.8
2.9
119.4
1011.6
17247.3
55383.1
1172.6
14.6
17.3
312.8
5488.1
205.1
74.6
345.8
30378.0
1810.0
199.6
669.5
20025.7
0.0
0.0
13720.7
27.6
507.1
10.9
13.5
284.4
119.3
358.3
210.1
76.7
1911.0
21112.5
14.8
1605.4
15192.9
19029.5
13472.5
12031.0
4.3
12745.5
1381.7
5439.7
49111.8
21839.2
13593.6
6.2
7.3
42.4
2726.7
2207.4
3941.3
286.2
51.5
103.2
339.6
12654.6
1102.2
138.8
361.1
31012.1
2619.0
1081.9
961.3
128740.6
3141.2
14603.8
11449.0
502.5
44754.8
572.6
2946.8
7563.1
7187.6
10300.1
38007.7
24293.0
19958.2
11857.7
8029.1
2077.5
15694.5
16320.9
19605.1
21328.1
5.0
8256.2
965.1
1646.5
55927.0
7491.9
6786.2
23.4
0.0
15.5
5350.1
1365.9
3022.0
158.5
16.7
98.9
30.6
8355.5
269.8
60.8
208.7
21922.1
244571.9
709.2
94041.3
43277.1
2392.3
2739.1
4787.0
153.2
12253.4
72.2
1060.6
25830.3
41428.6
34706.6
88045.1
74780.9
8302.2
1233.6
3.1
852.7
2589.7
6585.8
7012.8
14911.7
1.4
3829.5
969.6
3171.8
26178.5
11178.7
9783.6
7.7
0.0
0.0
1647.3
1734.9
3878.3
140.9
8.4
6.0
60.4
10295.0
134.8
46.0
291.7
16138.0
933.8
612.6
446.3
29919.6
2.2
0.0
14952.1
91.2
9120.7
41.9
589.0
14.9
7.7
9.8
106.6
17.8
448.1
1945.0
0.0
5154.0
2430.7
2870.3
26077.3
93709.4
3.7
6607.8
1347.7
719.2
67589.9
5510.0
4485.2
0.0
0.0
0.0
8906.0
4932.3
8753.6
292.7
0.0
184.8
517.1
7731.1
224.0
129.7
64.8
23827.8
2124.9
464.6
742.4
36915.9
10.4
0.0
5190.7
110.6
10163.9
157.9
701.0
6584.2
4732.8
6904.3
56393.3
40231.9
9223.2
628.0
1187.9
947.6
367.9
605.9
5830.6
16764.5
3.0
17951.9
567.3
186.5
4750.5
3199.8
2768.0
0.0
0.0
11.0
413.7
206.7
664.4
22.4
4.4
1.8
0.0
3003.7
13.2
67.0
15.5
7258.0
62253.7
418.9
27056.9
6221.7
0.0
0.0
1622.4
20.3
2151.8
11.4
258.1
911.0
691.4
1060.1
595.5
246.4
884.5
964.5
0.0
Continued
304
hsa-miR-206
hsa-miR-208
hsa-miR-210
hsa-miR-210N
hsa-miR-211
hsa-miR-212
hsa-miR-213
hsa-miR-214
hsa-miR-215
hsa-miR-216
hsa-miR-217
hsa-miR-218
hsa-miR-219
hsa-miR-220
hsa-miR-221
hsa-miR-222
hsa-miR-223
mmu-miR-224
hsa-miR-296
hsa-miR-299
hsa-miR-301
hsa-miR-302a
hsa-miR-302a*
hsa-miR-302b
hsa-miR-302c
hsa-miR-302c*
hsa-miR-302d
hsa-miR-320
hsa-miR-321
hsa-miR-323
hsa-miR-324-3p
hsa-miR-324-5p
hsa-miR-325
hsa-miR-326
hsa-miR-328
hsa-miR-330
hsa-miR-331
hsa-miR-335
hsa-miR-337
hsa-miR-338
hsa-miR-339
hsa-miR-340
hsa-miR-342
hsa-miR-345
hsa-miR-346
hsa-miR-361
3763.6
0.0
225.1
929.6
130.6
1185.6
9.2
23094.4
159.3
0.0
0.0
4346.8
0.0
0.0
2923.7
10729.8
44405.6
198.6
302.0
0.0
36.2
0.0
0.0
14.5
0.0
0.0
0.0
11807.7
26330.9
4.9
923.1
777.9
5.2
7.1
4159.3
49.8
6533.2
4309.0
0.0
1.6
98.9
437.8
3234.3
2255.5
26.5
1213.2
4413.2
0.0
1285.8
3261.5
9.0
1823.5
15.7
43803.0
125.7
0.0
7.2
16850.1
0.0
0.0
10950.7
36594.8
103591.4
52.9
825.8
0.0
390.3
0.0
0.0
607.7
0.0
0.0
2.9
22151.8
70277.0
4.3
6344.4
2767.0
5.6
19.0
7195.1
150.4
19854.1
3070.6
0.0
18.5
163.6
499.0
9529.3
2682.4
22.4
2362.1
31.2
0.0
551.0
1640.9
856.2
3837.4
468.9
640.2
4.4
0.0
3.7
22899.5
272.2
0.0
15420.3
47049.5
35812.0
0.0
422.3
0.0
329.8
23.6
0.0
713.5
39.9
0.0
29.1
9290.0
58569.1
4197.3
6447.7
5829.1
9.8
71.2
31722.8
1879.9
22978.2
3439.4
11.3
384.8
88.4
2348.8
33471.6
2340.2
1509.1
2507.0
192.2
9.4
963.5
2903.1
477.4
1208.4
20.6
54476.8
10.8
0.0
0.0
25310.3
0.0
0.0
5815.5
31550.2
77032.5
99.8
1137.2
7.7
365.7
0.0
0.0
554.9
0.0
0.0
5.2
25780.9
41675.5
20.8
5612.4
2047.6
1.8
8.8
7872.9
75.1
12182.3
2706.9
0.0
15.1
160.9
406.4
9353.6
3443.6
68.0
3358.8
95.2
0.0
1275.1
3385.0
7.0
960.1
37.7
19565.1
42464.7
0.0
0.0
5397.8
0.0
0.0
2840.5
12310.6
91189.0
13.4
386.7
0.0
347.3
0.0
0.0
1808.2
0.0
0.0
2.0
13686.5
47108.8
26.0
3059.5
1620.5
7.3
19.2
2438.4
69.9
9275.6
1838.2
0.0
92.7
118.8
299.1
8580.5
1819.1
21.8
1366.4
16.0
80.7
1755.3
4306.0
3.6
1221.4
47.8
13524.7
18.2
0.0
0.0
5478.5
0.0
0.0
11868.7
23973.3
61790.4
99.0
767.8
0.0
395.1
302.1
0.0
1899.7
277.1
0.0
1073.7
11110.1
41927.6
3.4
2398.5
1522.6
16.4
7.5
5631.1
55.8
12259.5
1414.7
3.5
23.1
55.0
524.7
6893.3
2049.9
34.1
1495.7
9.2
0.0
1375.8
3653.1
8.4
1206.9
69.7
12215.7
4.0
0.0
0.0
11158.6
0.0
0.0
3330.3
16077.2
368144.1
44.0
356.2
0.0
456.6
0.0
0.0
1218.9
0.0
0.0
1.7
5840.9
42983.2
4.0
3304.4
1626.2
4.8
39.7
3472.1
83.9
10546.8
3160.1
0.0
68.7
134.1
423.2
17643.4
2116.6
31.4
1108.2
2.6
0.0
485.2
1416.9
11.0
305.3
2.8
3942.7
85.7
0.0
0.0
424.5
0.0
0.0
347.3
1229.9
35692.1
9.5
95.0
0.0
48.6
0.0
0.0
875.2
0.0
0.0
0.0
6033.3
44266.8
0.0
713.4
500.3
2.8
3.4
1121.2
11.0
3279.8
676.8
0.0
10.9
15.9
285.7
3869.9
1055.3
4.7
412.4
Continued
305
hsa-miR-367
hsa-miR-368
hsa-miR-369
hsa-miR-370
hsa-miR-371
hsa-miR-372
hsa-miR-373
hsa-miR-374
hsa-miR-375
hsa-miR-376a
hsa-miR-377
hsa-miR-378
hsa-miR-379
hsa-miR-380-3p
hsa-miR-380-5p
hsa-miR-381
hsa-miR-382
hsa-miR-383
hsa-miR-384
hsa-miR-422b
hsa-miR-423
mmu-miR-424
hsa-miR-425
miRNA
hsa-let-7a
hsa-let-7b
hsa-let-7c
hsa-let-7d
hsa-let-7e
mmu-let-7f
hsa-let-7g
hsa-let-7i
hsa-miR-001
hsa-miR-007
hsa-miR-009
hsa-miR-009*
hsa-miR-010a
hsa-miR-010b
hsa-miR-015a
hsa-miR-015b
hsa-miR-016
hsa-miR-017-3p
hsa-miR-017-5p
hsa-miR-018
0.0
0.0
0.0
279.6
0.0
5.8
0.0
5440.2
487.3
213.6
0.0
852.5
216.9
0.0
0.0
8.9
458.2
66.3
0.0
580.4
455.0
0.0
552.8
0.0
0.0
0.0
551.7
18.4
13.3
0.0
20949.5
1796.3
506.5
4.8
1099.9
286.4
0.0
28.2
50.6
723.6
247.7
0.0
1011.3
1353.7
0.0
1149.0
Kidney
Esophagus
10077.2
29151.7
8611.3
659.4
486.1
0.0
13697.7
1200.0
809.4
197.0
1914.8
439.0
14974.1
12669.9
1268.6
7402.6
15483.3
97.5
2007.9
48.7
10852.6
35202.0
8797.4
1055.9
500.3
0.0
15929.7
1604.7
67661.9
303.1
1596.0
450.5
5618.0
1550.7
1836.0
9439.1
28366.5
126.1
1770.9
137.9
0.0
0.0
26.4
6234.0
1.9
7.9
0.0
9032.8
369.3
1163.4
7.0
129.9
1851.7
3.8
50.7
56.0
4505.6
3430.7
0.0
90.5
1478.0
0.0
1480.2
0.0
20.2
10.4
1451.8
0.0
8.7
0.0
20420.9
5491.6
1518.3
7.9
319.1
1220.9
1.5
15.1
54.8
1447.3
67.3
0.0
355.9
1907.0
1.7
1071.3
0.0
0.0
3.5
429.6
2.3
0.0
0.0
13029.0
26234.0
315.7
2.4
958.6
205.8
0.0
13.2
14.2
360.3
250.3
0.0
898.9
1185.0
0.0
719.5
Skeletal
Muscle
Ovary
13532.3
22384.8
5552.5
1223.3
195.5
0.0
15021.4
1377.8
1279818.8
89.9
450.7
126.7
1356.9
2195.8
2542.1
6141.3
30877.0
59.3
1125.5
39.9
25797.4
112522.3
42372.6
1118.9
943.8
0.0
24498.5
2466.0
10321.6
115.1
3437.1
654.0
5150.6
12014.8
1548.0
13089.9
34598.9
237.2
1708.2
60.9
186.9
8.2
0.0
205.4
1.7
13.1
0.0
12010.1
7.4
380.2
4.2
2385.2
201.8
0.0
0.0
42.1
445.8
0.0
0.0
1149.4
1320.1
0.0
574.5
0.0
0.0
0.0
262.4
0.0
9.1
0.0
12554.7
10114.3
270.6
0.0
224.6
121.1
0.0
0.0
7.0
377.6
0.0
0.0
178.2
1488.0
0.0
786.9
0.0
0.0
0.0
183.5
0.0
7.9
0.0
5028.7
2866.0
147.6
0.0
361.2
90.6
0.0
0.0
14.8
112.6
43.5
0.0
334.2
595.0
0.0
305.8
Pancreas
Placenta
Prostate
6375.2
37467.0
3276.7
293.9
124.0
0.0
6535.3
447.0
591.6
6013.2
443.7
114.0
2578.0
346.2
662.8
3486.1
5415.0
40.1
470.7
60.0
6828.2
9083.4
1701.0
344.8
486.7
0.0
5644.2
764.6
1580.3
438.4
573.4
172.1
776.7
217.8
2343.7
15774.0
35495.9
76.1
2618.5
222.4
11414.2
55437.8
39946.0
1087.9
545.6
0.0
18961.1
2028.6
40853.4
242.0
1950.2
521.1
2557.9
2358.1
1893.9
7981.5
37301.7
149.8
1776.5
72.4
Continued
306
hsa-miR-019a
hsa-miR-019b
hsa-miR-020
hsa-miR-021
hsa-miR-022
hsa-miR-023a
hsa-miR-023b
hsa-miR-023bN
hsa-miR-024
hsa-miR-025
hsa-miR-026aN
hsa-miR-026b
hsa-miR-026bN
hsa-miR-027a
hsa-miR-027b
hsa-miR-027bN
hsa-miR-028
hsa-miR-029a
hsa-miR-029b
hsa-miR-029c
hsa-miR-030a-3p
hsa-miR-030c
hsa-miR-030d
hsa-miR-030e-3p
hsa-miR-031
hsa-miR-032
hsa-miR-033
hsa-miR-034a
hsa-miR-034aN
hsa-miR-034b
hsa-miR-034b
hsa-miR-092N
hsa-miR-093
hsa-miR-095
hsa-miR-096
hsa-miR-098
hsa-miR-099a
hsa-miR-099b
hsa-miR-100
hsa-miR-101
hsa-miR-103
hsa-miR-105
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-108
9996.6
53795.0
64255.4
34629.9
1529.2
432.3
3969.9
28983.4
160481.7
2258.2
151615.3
52285.7
39639.5
18602.1
8606.2
85127.9
3194.7
27559.5
655.2
10731.8
38859.0
232146.8
21134.6
2705.1
10786.1
3166.7
43.2
196.5
2318.0
0.0
0.0
26746.7
12930.1
2089.9
513477.8
992.0
21757.2
7743.0
12638.3
351.7
4380.2
561.1
48954.8
4108.8
191.1
0.0
11367.2
66636.8
85026.6
48966.0
1887.2
688.4
6327.8
48382.4
328445.2
2630.2
167946.2
51517.9
38831.0
40746.0
19131.4
147151.2
17191.3
22459.9
876.6
10086.8
11564.7
77726.7
11119.3
3864.6
20549.9
1862.4
21.6
294.8
4433.9
38.7
21.2
30797.3
21802.2
3895.8
624367.1
666.0
20340.1
4869.8
14558.9
407.2
3324.3
600.7
86632.0
5031.2
18.7
0.0
6829.4
42202.9
49096.9
12954.5
7995.9
638.2
2751.7
40848.5
291812.1
2466.4
179909.3
45431.2
30671.5
37020.8
9358.3
71229.8
5864.5
35949.6
1201.0
12606.5
17926.4
108072.7
19057.6
7609.2
191.1
1760.5
0.0
100.6
1460.1
0.0
0.0
33938.5
11116.9
65316.6
508885.3
946.4
10295.2
1652.5
5442.3
771.3
1566.7
780.9
104687.1
2933.2
24.6
0.0
10807.9
73471.4
78862.7
36211.3
1424.4
560.9
5491.9
38443.1
253145.5
3937.8
354910.3
75479.5
60203.7
19222.5
12034.9
115354.7
7171.9
30619.2
413.5
7969.7
8570.8
77643.7
11524.2
5815.4
5520.9
2262.8
0.0
651.1
6753.0
699.6
564.8
56089.7
19528.7
1276.1
377259.3
979.4
66443.6
8065.5
47843.3
554.2
3877.9
1454.4
66511.4
4837.2
19.8
0.0
5883.2
31443.4
23741.2
14739.6
464.5
85.8
1578.6
8720.7
69317.4
1259.3
49692.7
20646.2
15160.8
5523.9
2810.4
43815.3
1472.6
10146.5
345.7
9136.2
8775.9
35355.1
6671.0
1777.2
1183.5
1217.2
0.0
160.1
2307.6
9.2
0.0
13585.8
3984.0
926.6
866530.1
272.5
4039.7
1309.6
2874.4
183.3
800.8
484.9
123234.3
1629.4
3.8
0.0
10765.5
66866.9
77963.5
43467.8
3404.3
1322.3
3550.7
19340.6
529861.1
3653.9
107719.7
69269.9
57317.5
66255.5
7210.3
77943.7
3582.7
17298.4
618.0
3501.8
21285.2
82507.2
42985.5
1646.9
6564.3
2969.5
0.0
207.0
2615.5
72.9
62.1
22934.4
35180.8
516.1
585776.3
386.0
36189.8
9065.5
31058.1
306.5
5139.5
874.9
75586.7
6720.4
16.1
0.0
10843.4
73188.9
63874.3
67656.1
2888.2
631.5
6281.4
32115.5
403163.3
2536.2
212595.6
72012.2
60006.1
33213.1
23503.6
200770.2
10413.5
32934.6
564.1
13267.5
10377.2
64814.7
12327.1
3516.1
15207.5
1856.0
4.4
640.3
6223.0
23.6
30.5
22135.0
21769.4
2257.0
436806.1
661.6
45368.5
3968.1
29408.7
545.4
3858.6
830.5
48471.2
4213.4
20.1
0.0
Continued
307
hsa-miR-122a
hsa-miR-124a
hsa-miR-125a
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
hsa-miR-127
hsa-miR-128a
mmu-miR-129-2
hsa-miR-130a
hsa-miR-130aN
hsa-miR-130b
hsa-miR-132
hsa-miR-133a
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
mmu-miR-137
hsa-miR-139
hsa-miR-140
hsa-miR-141
hsa-miR-142-3p
hsa-miR-142-5p
hsa-miR-143
hsa-miR-145
hsa-miR-146
hsa-miR-146b
hsa-miR-147
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-152
hsa-miR-152N
hsa-miR-153
hsa-miR-154
hsa-miR-154*
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-182
hsa-miR-183
hsa-miR-184
hsa-miR-186
hsa-miR-187
0.0
0.0
83936.9
29566.1
165237.6
18384.7
2293.7
0.0
0.0
2976.6
9857.1
59.3
6702.0
130.0
183.3
8553.8
4816.5
0.0
252.1
24294.8
2172.7
7731.1
751.4
1425.1
2130.0
14360.1
20360.1
4.1
6108.6
1419.0
1378.5
19990.7
7578.4
5980.9
6.5
0.0
0.0
1773.9
3718.4
6496.3
251.3
3.7
187.5
243.9
5919.9
627.5
1.7
0.0
59566.7
54132.1
185440.2
22526.1
2213.2
0.0
0.0
1998.4
9536.9
68.4
5779.3
16566.8
184.8
381.6
2086.2
53.9
291.0
20568.4
1802.4
15795.7
1486.3
20497.1
34480.2
14523.5
15067.3
3.7
4135.1
1070.6
3223.9
39163.2
10433.1
9830.7
5.0
0.0
0.0
3529.1
1043.0
2433.4
128.1
9.9
228.1
11.7
8622.7
1391.6
0.0
0.0
21498.7
31899.8
175040.4
23500.2
2530.1
55.9
0.0
462.4
2427.9
40.7
4004.6
325436.3
210.4
180.9
45.1
0.0
201.4
19952.9
20.1
6664.7
689.5
1682.6
1870.8
8066.1
6327.2
4.7
2462.4
594.1
815.1
29793.8
12837.4
9782.3
20.0
0.0
4.6
1418.5
764.9
1477.3
59.3
7.8
115.6
63.2
8137.3
0.0
0.0
0.0
127804.7
219811.6
167043.0
19513.5
14586.1
0.0
15.3
3103.2
18680.6
74.9
8949.3
1197.5
1424.4
2348.4
1990.3
17.4
209.3
32248.7
421.4
10370.9
992.2
8121.5
11749.6
6477.8
33648.9
4.3
11106.9
853.0
3076.7
20824.6
9650.3
6787.7
14.1
0.0
10.8
3979.9
1591.5
5190.6
178.1
17.5
17.6
9.7
9926.3
117.3
0.0
0.0
15482.9
13399.2
44896.8
5942.3
2624.8
0.0
18.8
897.6
3362.4
491.6
3059.3
67.3
257.3
993.9
613.2
30.0
87.0
6150.9
13451.8
5628.6
582.5
538.2
857.6
3699.3
15368.4
4.0
68386.1
557.7
477.5
8550.5
3333.6
2093.2
10.0
0.0
23.0
515.6
398.9
1359.8
41.5
6.6
170.5
77.4
2981.2
30.3
3.1
0.0
126209.9
35933.5
242580.8
17413.4
17410.7
0.0
0.0
4264.5
18147.6
303.4
2693.8
263.8
989.5
518.6
13871.2
253.3
141.2
15014.3
6911.1
19655.9
1905.4
2523.0
3829.0
5626.3
5973.1
4.0
3507.9
2028.9
10109.4
7456.5
8476.2
7430.4
0.0
4.0
79.7
897.6
8781.7
5851.4
442.0
7.4
233.8
821.5
6981.5
0.0
0.0
0.0
56673.1
111118.5
160471.4
20759.2
6928.7
0.0
9.3
3658.9
20919.7
96.0
9394.4
9766.0
672.5
5918.2
1899.3
53.0
282.9
21493.0
2594.3
12231.4
2023.4
19912.3
15745.3
13006.3
20290.1
4.1
7622.4
1342.0
5656.0
24593.9
13787.5
11156.5
2.5
0.0
13.3
2597.6
813.9
2906.4
181.2
15.5
238.7
88.3
13496.8
1554.1
Continued
308
hsa-miR-188b
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196b
hsa-miR-197
hsa-miR-199a
hsa-miR-199a*
hsa-miR-199b
hsa-miR-199-s
hsa-miR-200a
hsa-miR-200b
hsa-miR-200bN
hsa-miR-200c
hsa-miR-200cN
hsa-miR-203
hsa-miR-204
hsa-miR-205
hsa-miR-206
hsa-miR-208
hsa-miR-210
hsa-miR-210N
hsa-miR-211
hsa-miR-212
hsa-miR-213
hsa-miR-214
hsa-miR-215
hsa-miR-216
hsa-miR-217
hsa-miR-218
hsa-miR-219
hsa-miR-220
hsa-miR-221
hsa-miR-222
hsa-miR-223
mmu-miR-224
hsa-miR-296
hsa-miR-299
hsa-miR-301
hsa-miR-302a
hsa-miR-302a*
hsa-miR-302b
93.2
955.9
18363.5
74764.3
564.7
33424.2
24047.2
5600.2
1018.1
4631.5
105.0
7946.2
18.0
681.0
27531.9
20632.3
22674.2
26304.8
15344.7
2843.2
150543.5
90.7
107.1
0.0
1134.9
3026.5
238.0
907.8
48.3
10752.2
110.3
0.0
16.1
9104.3
0.0
0.0
4475.8
15962.5
27882.7
13.4
230.0
0.0
443.8
0.0
0.0
545.0
88.4
113.5
17658.8
2593.8
1020.3
931.4
48198.7
97.3
0.0
4747.1
121.6
11030.4
85.6
827.6
5606.7
7950.4
6163.1
35266.9
24488.4
104075.3
1114.1
23002.5
16885.0
0.0
3106.9
6970.2
5.7
842.9
20.7
18963.3
174.5
0.0
5.8
10346.9
0.0
0.0
4872.9
17110.8
83327.5
53.7
406.2
0.0
270.2
0.0
0.0
808.2
100.4
155.4
11737.2
1471.9
608.9
583.5
23501.7
6403.9
619.3
6150.8
45.9
6974.5
85.6
382.7
54.4
22.9
60.6
226.5
71.8
110.9
1135.6
22.8
468142.2
5.1
777.8
1610.5
2.8
838.7
0.0
8707.0
18.2
0.0
8.1
2029.4
0.0
0.0
1830.1
6970.3
36094.5
13.5
789.9
0.0
94.2
17.6
0.0
1018.0
109.9
117.9
31599.3
3087.0
825.2
1130.3
123747.4
3241.0
78.3
7819.8
394.6
38622.2
142.5
3010.3
2694.3
5605.7
7725.5
17442.3
14630.4
2067.3
12368.8
0.0
97.0
0.0
445.9
1316.1
6.9
1811.5
11.2
49907.9
9.7
0.0
0.0
7246.4
0.0
0.0
5095.0
35996.9
55742.6
20.6
661.4
0.0
216.0
0.0
0.0
959.5
77.0
73.5
6565.2
25192.4
405.1
4766.0
10053.2
35.9
0.0
1989.1
40.5
3480.8
56.6
300.4
14205.7
7348.0
13569.1
96773.8
59080.5
564.5
1363.1
9.8
15.8
0.0
159.2
752.5
2.3
651.9
1.9
4655.7
365.1
999.5
179.8
2279.8
2.9
0.0
975.3
2972.8
28922.8
50.6
211.9
0.0
276.2
0.0
0.0
460.7
92.5
96.7
34049.3
4889.5
617.4
1846.8
8734.2
800.0
333.8
3278.5
233.6
12258.2
118.1
948.2
125.4
37.8
578.7
100754.4
73356.0
17358.7
2951.2
3353.5
12.2
0.0
1676.4
4835.9
46.8
575.7
151.8
9765.2
5.5
0.0
16.2
6245.7
0.0
0.0
15978.7
41450.2
161905.5
422.0
336.7
8.2
624.7
0.0
0.0
701.3
82.8
98.6
22783.2
6287.3
1182.6
1577.5
60740.4
1884.4
2756.1
5382.6
216.4
23259.0
134.5
1590.9
9940.1
11594.3
17601.6
49800.7
28981.4
4062.3
8591.4
15420.9
27381.7
15.7
720.9
2448.9
11.0
1716.9
11.2
33002.8
240.5
0.0
0.0
12398.5
0.0
0.0
12052.3
35669.2
65951.0
76.0
496.1
3.6
281.5
0.0
0.0
759.8
Continued
309
hsa-miR-302c
hsa-miR-302c*
hsa-miR-302d
hsa-miR-320
hsa-miR-321
hsa-miR-323
hsa-miR-324-3p
hsa-miR-324-5p
hsa-miR-325
hsa-miR-326
hsa-miR-328
hsa-miR-330
hsa-miR-331
hsa-miR-335
hsa-miR-337
hsa-miR-338
hsa-miR-339
hsa-miR-340
hsa-miR-342
hsa-miR-345
hsa-miR-346
hsa-miR-361
hsa-miR-367
hsa-miR-368
hsa-miR-369
hsa-miR-370
hsa-miR-371
hsa-miR-372
hsa-miR-373
hsa-miR-374
hsa-miR-375
hsa-miR-376a
hsa-miR-377
hsa-miR-378
hsa-miR-379
hsa-miR-380-3p
hsa-miR-380-5p
hsa-miR-381
hsa-miR-382
hsa-miR-383
hsa-miR-384
hsa-miR-422b
hsa-miR-423
mmu-miR-424
hsa-miR-425
0.0
0.0
3.8
7752.2
31941.4
0.0
2956.4
2372.4
5.3
4.9
5976.3
61.1
12206.4
1374.9
0.0
12.1
118.5
301.9
5755.4
2649.5
13.1
1397.2
0.0
13.9
0.0
225.2
0.0
10.5
12.9
13420.1
305.0
197.2
2.6
432.7
67.3
0.0
0.0
16.1
163.5
3.4
0.0
352.7
1400.0
0.0
635.4
0.0
0.0
9.1
15311.9
67090.6
2.8
3860.1
1886.1
6.2
14.9
4604.5
75.6
9100.6
790.5
0.0
43.8
114.8
358.9
7442.7
1851.5
26.4
1506.8
0.0
0.0
3.8
420.5
0.0
2.3
0.0
13060.8
4180.2
230.1
0.0
980.3
184.5
0.0
12.2
15.4
291.5
38.3
0.0
496.4
1245.5
0.0
667.5
0.0
0.0
11.1
6563.2
53878.2
2.5
2558.7
1014.0
3.1
7.6
6492.9
21.9
18822.6
1248.8
15.6
2.7
60.3
464.4
4682.1
3362.1
26.5
1679.6
14.5
0.0
3.7
387.3
2.8
20.0
0.0
13121.2
46.6
280.3
0.0
7679.2
206.0
0.0
0.0
12.8
236.5
56.7
0.0
2022.1
1513.1
0.0
335.4
0.0
0.0
3.1
18948.2
14942.5
36.3
3734.3
1215.6
2.4
9.2
11300.5
43.1
10784.7
2915.5
0.0
12.3
243.3
403.0
10006.2
3539.3
41.3
2573.1
0.0
0.0
0.0
1183.1
8.0
37.5
2.2
16767.3
1342.3
1039.9
0.0
276.6
944.6
0.0
0.0
46.4
1555.9
2147.4
0.0
263.8
1914.2
0.0
1157.6
0.0
0.0
7.1
4799.1
13477.7
10.2
530.8
375.7
15.8
2.9
1722.7
36.7
3495.0
1794.1
0.0
32.9
87.4
135.5
1623.6
503.6
9.6
427.6
0.0
0.0
0.0
210.7
3.1
0.0
0.0
4290.1
219706.0
226.4
0.0
89.1
131.5
0.0
0.0
11.9
397.8
28.3
0.0
0.0
562.9
0.0
0.0
0.0
0.0
10.6
40129.9
57550.3
387.2
2493.2
2468.5
2.1
6.7
1595.9
126.2
10122.7
15039.1
10.9
0.0
109.5
323.3
4974.7
792.1
13.9
1437.2
0.0
9.6
56.5
2499.9
769.2
2795.4
246.2
14582.2
384.2
5411.4
10.5
194.8
1511.9
3.2
64.4
51.8
4824.9
7.8
0.0
183.1
918.5
0.0
1096.4
10.0
0.0
15.2
15806.6
60924.3
22.0
5522.6
2597.0
4.2
23.0
4192.2
107.4
9716.4
1882.7
1.8
0.0
156.8
226.7
8559.6
2745.3
16.0
2023.6
0.0
0.0
8.5
762.3
1.5
7.9
0.0
14597.9
19148.5
868.2
0.0
442.2
365.3
0.0
40.4
64.9
829.5
454.8
0.0
464.4
1224.5
0.0
1270.6
Continued
310
miRNA
Spleen
hsa-let-7a
hsa-let-7b
hsa-let-7c
hsa-let-7d
hsa-let-7e
mmu-let-7f
hsa-let-7g
hsa-let-7i
hsa-miR-001
hsa-miR-007
hsa-miR-009
hsa-miR-009*
hsa-miR-010a
hsa-miR-010b
hsa-miR-015a
hsa-miR-015b
hsa-miR-016
hsa-miR-017-3p
hsa-miR-017-5p
hsa-miR-018
hsa-miR-019a
hsa-miR-019b
hsa-miR-020
hsa-miR-021
hsa-miR-022
hsa-miR-023a
hsa-miR-023b
hsa-miR-023bN
hsa-miR-024
hsa-miR-025
hsa-miR-026aN
hsa-miR-026b
hsa-miR-026bN
hsa-miR-027a
hsa-miR-027b
hsa-miR-027bN
hsa-miR-028
hsa-miR-029a
hsa-miR-029b
hsa-miR-029c
hsa-miR-030a-3p
hsa-miR-030c
hsa-miR-030d
hsa-miR-030e-3p
hsa-miR-031
7188.0
29589.8
1544.5
717.8
338.6
0.0
15392.2
1758.4
802.7
243.6
151.4
57.8
5052.3
2819.1
2003.3
17069.3
35112.5
134.4
1643.0
142.5
11025.7
59523.0
53992.0
33698.3
1344.1
329.1
2001.0
10430.2
135165.6
3283.9
91386.4
42712.6
35342.5
14311.5
2035.7
28056.9
3673.5
18027.7
421.5
12558.2
4403.2
42349.1
6646.3
2521.8
446.7
Small
Intestine
4012.1
16506.7
1785.7
614.8
185.8
0.0
10093.1
924.0
6667.4
1766.9
1744.0
367.5
5795.6
889.3
1259.6
8944.3
11330.4
49.8
1641.7
148.8
12603.2
65503.5
53027.9
36337.4
1336.1
193.9
2147.4
15618.8
113191.7
2823.5
53577.6
28077.1
21028.5
13071.9
2786.8
32865.5
6089.0
15160.2
308.6
5621.1
5209.2
39768.1
5914.5
2166.9
51594.6
Trachea
6029.4
26143.2
8367.4
552.2
220.0
0.0
11957.5
1304.5
4256.5
226.7
667.8
128.7
2596.3
966.0
1495.3
6324.8
17248.6
65.4
1107.6
77.0
6000.6
40849.2
36868.9
30273.4
1148.1
397.0
2506.5
15565.6
149177.4
2001.6
94348.5
32056.6
25032.8
13803.0
3603.9
47071.3
3615.0
16732.3
346.1
6628.4
6913.0
40682.7
6200.8
2369.9
13128.3
Thymus
5546.6
23383.8
8127.5
727.0
210.4
0.0
15582.5
1401.5
604.4
443.0
290.8
58.8
962.7
1072.1
3869.9
29926.0
51925.3
147.1
6085.3
361.3
20954.7
109027.2
113277.0
15321.4
548.0
216.7
905.1
4528.1
57463.7
8960.0
92098.5
26702.4
21607.4
6635.1
879.5
14822.8
2916.1
13944.0
199.6
7423.8
2683.5
42625.7
5862.9
2983.6
2934.0
Normal
B cell
Thyroid
Testes
10310.4
27150.6
12071.4
1532.3
325.8
0.0
18600.4
6719.3
55121.3
12161.6
206.8
93.0
1277.9
808.7
1295.6
8386.4
27178.6
109.7
909.7
51.0
5815.0
38453.6
42535.0
19286.2
1274.8
338.1
2398.2
11160.2
140186.3
2232.6
116772.8
46444.2
35599.8
11155.8
2855.4
57955.7
2028.8
27718.2
461.2
10471.9
9835.1
65687.9
10111.4
1955.5
7032.7
8138.0
40081.9
17833.5
685.4
503.3
0.0
272.7
996.6
190.0
30.8
1477.7
437.8
2002.1
5993.6
194.0
15926.4
8.1
19.1
423.3
171.8
12254.9
75554.6
61647.5
16718.0
1458.3
11.3
2442.6
19383.9
126345.7
2860.6
95968.8
31198.9
21765.8
15002.0
4397.4
37981.5
3071.1
19602.0
412.0
5415.1
7825.5
47202.7
5147.6
4032.3
8456.3
707.8
2611.6
84.3
227.8
3.3
0.0
113.2
281.8
0.0
0.0
25.5
0.0
16.0
0.0
64.7
5266.7
6.3
4.1
60.9
54.0
1590.5
12453.0
12383.0
951.4
12.6
0.0
23.0
255.0
5594.1
1394.8
13895.1
4599.0
4788.9
332.2
16.6
320.9
948.6
6143.3
18.2
3734.3
65.6
12270.8
1624.4
780.9
47.5
Continued
311
hsa-miR-032
hsa-miR-033
hsa-miR-034a
hsa-miR-034aN
hsa-miR-034b
hsa-miR-034b
hsa-miR-092N
hsa-miR-093
hsa-miR-095
hsa-miR-096
hsa-miR-098
hsa-miR-099a
hsa-miR-099b
hsa-miR-100
hsa-miR-101
hsa-miR-103
hsa-miR-105
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-108
hsa-miR-122a
hsa-miR-124a
hsa-miR-125a
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
hsa-miR-127
hsa-miR-128a
mmu-miR-129-2
hsa-miR-130a
hsa-miR-130aN
hsa-miR-130b
hsa-miR-132
hsa-miR-133a
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
mmu-miR-137
hsa-miR-139
hsa-miR-140
hsa-miR-141
hsa-miR-142-3p
hsa-miR-142-5p
hsa-miR-143
hsa-miR-145
2132.0
1.9
102.7
1435.5
0.0
8.3
25844.3
14986.0
8834.0
582403.0
479.0
2407.8
3347.5
2104.1
293.4
4569.3
803.3
54220.8
4713.2
4.8
0.0
0.0
0.0
41162.0
6653.0
306194.7
38173.2
4796.2
0.0
0.0
1917.0
6911.0
68.5
4483.9
80.5
539.0
56.6
767.0
0.0
1834.1
17959.5
30.3
146286.3
4233.6
1584.7
2557.1
1688.4
0.0
120.2
1480.6
9.8
0.0
17971.4
16394.2
846.4
696132.5
242.9
5332.0
2103.5
4611.9
209.6
2530.3
601.3
84168.8
4221.2
30.5
0.0
0.0
0.0
27795.2
12497.4
88518.2
11128.4
2027.7
0.0
0.0
1101.0
4431.9
134.5
4945.8
1198.4
184.7
118.9
1925.7
146.1
188.0
11373.3
6699.2
25459.5
1913.4
6454.6
7942.2
1025.0
2.5
249.6
2334.7
1360.3
1229.8
17433.3
10689.2
921.3
573402.1
327.2
18839.4
2669.4
10789.2
181.4
2331.1
535.2
64727.1
2809.5
5.3
0.0
0.0
0.0
36905.6
36477.7
131469.8
14712.6
3171.5
0.0
0.0
790.0
4738.3
41.6
4855.4
813.4
338.1
485.6
4563.4
2.5
101.2
204.3
3278.0
29780.7
2503.2
2963.4
4413.5
2375.5
17.5
203.5
2293.3
36.7
59.2
49898.4
35764.6
997.9
396347.7
393.5
24320.9
1868.4
16583.7
303.0
2785.6
1534.7
46545.4
9199.3
16.5
0.0
0.0
0.0
28228.3
44440.7
94212.0
11552.4
2861.9
7.7
0.0
1908.7
9862.4
284.9
7259.9
130.0
198.4
402.8
565.6
21.0
161.3
13010.9
1627.4
158461.1
8236.6
695.2
1115.7
1514.2
0.0
230.2
2557.4
0.0
0.0
15906.2
11547.8
5282.3
837447.8
670.1
27472.4
3480.2
16845.5
233.8
2637.6
454.2
109061.8
2872.2
45.9
0.0
0.0
0.0
48935.3
56981.0
201081.6
18110.1
796.8
0.0
0.0
2000.5
15310.3
97.6
2621.9
9296.2
77.8
49202.8
20418.0
20.3
163.0
9317.8
2922.3
4822.3
632.2
1085.5
1238.0
1657.4
67.8
500.8
5580.1
967.2
870.1
26066.3
10777.9
750.4
367914.0
446.4
438.5
4864.6
17508.3
246.1
3257.8
870.0
30324.5
296.9
14.1
0.0
3.4
0.0
67233.4
73863.3
98601.5
12545.3
16842.7
0.0
14.9
1752.8
7786.1
44.9
7860.5
921.1
2017.9
4748.0
12368.7
0.0
163.7
19716.7
528.5
7443.5
810.8
3651.9
5101.9
113.8
4.6
3.7
14.9
0.0
0.0
19970.3
2854.1
116.5
471842.4
31.4
0.0
31.3
0.0
49.9
549.2
567.4
52471.3
76.2
0.0
0.0
0.0
0.0
421.7
17.5
1735.0
203.3
18.0
0.0
0.0
26.9
107.8
2.6
131.0
4.6
4.0
5.6
15.8
0.0
9.7
1962.3
6.7
22691.4
3165.1
0.0
13.9
Continued
312
hsa-miR-146
hsa-miR-146b
hsa-miR-147
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-152
hsa-miR-152N
hsa-miR-153
hsa-miR-154
hsa-miR-154*
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-182
hsa-miR-183
hsa-miR-184
hsa-miR-186
hsa-miR-187
hsa-miR-188b
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196b
hsa-miR-197
hsa-miR-199a
hsa-miR-199a*
hsa-miR-199b
hsa-miR-199-s
hsa-miR-200a
hsa-miR-200b
hsa-miR-200bN
hsa-miR-200c
hsa-miR-200cN
hsa-miR-203
hsa-miR-204
hsa-miR-205
hsa-miR-206
hsa-miR-208
hsa-miR-210
31506.8
24546.0
5.5
2890.7
732.6
608.3
185337.1
2905.4
2548.7
27.1
0.0
10.3
9587.2
2864.2
6888.1
203.0
14.9
4.2
0.0
5712.4
47.1
66.9
80.9
18796.1
1999.3
406.1
873.4
25739.8
29.2
0.0
6054.1
109.7
8462.3
9.0
656.1
40.8
25.4
93.3
388.2
277.4
37.0
4203.4
0.0
0.0
0.0
96.3
8747.9
14637.0
9.3
5330.2
702.0
1041.6
38465.6
3528.6
2686.0
47.1
0.0
0.0
3444.0
679.7
2880.2
69.8
12.7
84.5
0.0
5493.7
623.2
76.1
81.4
11890.3
334215.6
397.8
145110.3
20908.2
81.3
0.0
3795.1
59.2
5443.0
43.9
429.8
19355.7
15116.7
19970.8
85635.8
54586.0
1843.8
419.8
0.0
6.1
0.0
628.7
34051.8
31232.0
1.7
11556.6
669.9
1527.8
73851.0
6634.1
4606.5
7.9
0.0
9.2
5880.1
434.6
2632.8
61.8
4.0
94.0
11.7
5090.0
360.9
66.3
45.7
13267.9
1016.8
334.5
382.5
24917.6
54.7
0.0
3340.2
60.9
10428.7
73.0
799.8
18843.7
23077.8
27034.9
87730.1
57431.8
2203.8
1698.9
6503.6
900.7
0.0
292.1
28862.9
20135.9
0.0
6657.1
613.6
9367.8
212825.0
4173.9
2716.9
2.7
0.0
0.0
17280.7
25794.1
30230.0
1060.0
30.4
922.1
85.1
5504.8
9.0
109.0
42.3
21718.1
1976.3
403.5
759.0
17580.3
526.5
462.3
5434.1
51.6
4698.0
60.2
334.3
9651.6
5989.6
13968.8
18637.4
10550.2
6554.3
2377.3
20956.2
1740.0
0.0
805.6
8255.7
13911.8
3.1
4848.1
1487.7
311.0
18399.5
8561.5
6390.5
10.2
0.0
0.0
2354.8
3552.4
7682.4
303.4
15.6
228.7
17.0
3782.9
24.2
81.9
106.5
16439.0
3082.5
443.8
1052.3
21127.0
23.3
0.0
2102.4
47.0
5608.8
61.3
399.7
7837.4
5223.9
11593.9
78343.7
53186.4
5302.9
3387.7
64.5
14906.2
0.0
211.4
7288.2
15510.6
0.0
6089.4
932.2
3450.1
10549.0
7568.6
5576.3
3.8
0.0
4.2
156.7
10.2
3314.3
0.0
18.1
0.0
74.9
7323.1
924.7
82.4
74.7
2852.0
2958.3
283.7
1117.2
4664.0
1263.9
4.3
3514.2
242.9
1111.8
4.4
250.9
371.5
1139.1
103.1
7330.2
556.0
115.7
26781.3
654.9
340.1
0.0
19.0
8308.8
1115.6
0.0
35.1
55.3
8.5
427307.7
116.1
59.3
0.0
0.0
0.0
653.1
2.8
977.0
0.0
24.8
0.0
0.0
684.5
0.0
55.8
0.0
1060.2
323.3
97.7
173.4
34.1
0.0
0.0
1348.1
0.0
0.0
0.0
0.0
2.0
13.6
3.2
503.1
37.1
0.0
0.0
0.0
0.0
0.0
1.7
Continued
313
hsa-miR-210N
hsa-miR-211
hsa-miR-212
hsa-miR-213
hsa-miR-214
hsa-miR-215
hsa-miR-216
hsa-miR-217
hsa-miR-218
hsa-miR-219
hsa-miR-220
hsa-miR-221
hsa-miR-222
hsa-miR-223
mmu-miR-224
hsa-miR-296
hsa-miR-299
hsa-miR-301
hsa-miR-302a
hsa-miR-302a*
hsa-miR-302b
hsa-miR-302c
hsa-miR-302c*
hsa-miR-302d
hsa-miR-320
hsa-miR-321
hsa-miR-323
hsa-miR-324-3p
hsa-miR-324-5p
hsa-miR-325
hsa-miR-326
hsa-miR-328
hsa-miR-330
hsa-miR-331
hsa-miR-335
hsa-miR-337
hsa-miR-338
hsa-miR-339
hsa-miR-340
hsa-miR-342
hsa-miR-345
hsa-miR-346
hsa-miR-361
hsa-miR-367
hsa-miR-368
hsa-miR-369
372.0
5.8
1037.6
43.9
13523.0
7.9
0.0
0.0
9912.9
0.0
0.0
1540.5
5126.6
552278.1
11.2
272.8
0.0
250.8
0.0
0.0
2120.8
29.2
0.0
5.6
7978.0
26118.3
15.3
2292.7
948.8
11.3
22.5
2818.1
68.1
5606.3
3121.3
0.0
17.2
99.5
387.8
14736.1
1717.8
4.8
933.3
0.0
6.9
15.2
2714.2
0.0
961.9
7.7
9057.7
81909.2
0.0
1.6
3506.1
0.0
0.0
2277.8
8085.8
36160.2
44.0
176.6
0.0
218.1
0.0
0.0
530.9
0.0
0.0
8.6
8170.9
46330.9
5.1
2218.2
695.9
16.3
30.0
1591.3
48.1
4158.5
1186.7
3.5
33.8
48.1
211.4
6954.1
1539.2
22.6
599.5
0.0
0.0
2.0
1394.3
11.0
1154.0
5.2
12764.9
8.2
0.0
0.0
6353.6
0.0
0.0
2233.2
9507.8
136543.5
21.6
376.7
0.0
121.6
0.0
0.0
1635.4
21.3
0.0
11.7
9180.7
38108.7
0.0
2019.2
614.3
5.7
6.2
2808.0
47.1
5257.8
1749.5
0.0
4.4
81.6
227.8
6739.3
2590.8
26.8
714.9
0.0
0.0
13.4
1963.6
165.4
1281.4
568.4
7316.9
12.1
0.0
0.0
3903.7
0.0
0.0
2185.1
8978.5
56461.9
39.5
544.0
0.0
454.8
0.0
0.0
1051.2
0.0
0.0
0.0
7904.8
31798.8
5.4
2665.2
787.0
1.4
18.7
3789.6
73.5
5986.9
1489.8
0.0
14.9
58.4
136.1
28735.9
2503.0
24.5
928.0
0.0
0.0
3.0
1000.4
0.0
446.8
37.1
10088.8
3.7
0.0
0.0
4072.5
0.0
0.0
1679.7
7707.1
40258.8
44.1
505.2
0.0
112.7
0.0
0.0
1298.5
18.8
0.0
7.0
7391.1
27618.0
0.0
3361.8
965.5
7.0
4.4
3425.7
43.8
8418.7
1427.7
0.0
4.3
48.9
202.2
3235.3
7157.1
66.2
762.2
0.0
0.0
3.6
433.2
0.0
1135.8
3.2
7671.3
122.3
0.0
3.0
3232.2
0.0
0.0
2093.5
6393.4
24194.0
21.9
544.0
10.1
309.3
0.0
0.0
721.9
0.0
0.0
0.0
7702.4
29289.2
146.9
2869.6
803.9
3.9
9.7
5468.0
37.9
6567.2
2173.8
2.2
0.0
84.9
266.7
5622.7
2666.1
33.8
1149.3
0.0
4.7
15.1
33.2
0.0
301.9
0.0
0.0
0.0
0.0
0.0
20.5
0.0
0.0
254.0
1345.5
59716.9
0.0
102.5
0.0
34.4
0.0
0.0
727.2
17.3
0.0
0.0
3927.5
4207.4
0.0
402.0
17.3
16.1
0.0
574.9
21.6
1511.1
48.3
0.0
0.0
83.4
31.3
9338.8
281.1
4.1
179.4
0.0
0.0
0.0
Continued
314
hsa-miR-370
hsa-miR-371
hsa-miR-372
hsa-miR-373
hsa-miR-374
hsa-miR-375
hsa-miR-376a
hsa-miR-377
hsa-miR-378
hsa-miR-379
hsa-miR-380-3p
hsa-miR-380-5p
hsa-miR-381
hsa-miR-382
hsa-miR-383
hsa-miR-384
hsa-miR-422b
hsa-miR-423
mmu-miR-424
hsa-miR-425
1160.8
13.5
3.8
0.0
13252.1
65.4
698.0
0.0
132.8
288.2
0.0
11.2
3.2
1029.7
9.3
0.0
77.1
1292.8
0.0
607.3
235.8
3.5
2.6
0.0
9719.9
28817.5
168.8
0.0
452.8
111.6
0.0
0.0
15.8
326.3
114.8
0.0
660.5
878.4
0.0
456.4
435.0
1.7
6.4
0.0
9234.4
23824.7
293.7
0.0
233.2
196.7
0.0
0.0
19.5
439.4
102.2
0.0
141.5
1008.1
0.0
548.1
315
391.2
9.2
7.7
0.0
8584.8
1911.2
188.7
0.0
176.6
143.3
0.0
0.0
33.6
298.5
1070.1
0.0
99.6
1552.9
0.0
446.2
98.7
7.5
2.7
0.0
9448.3
775.9
111.0
0.0
425.3
53.9
0.0
1.9
1.9
133.5
4.6
0.0
142.8
1181.7
0.0
571.9
1563.8
98.6
487.7
54.0
11283.3
1793.2
930.3
13.5
78.9
979.2
0.0
49.0
38.3
1690.1
947.8
0.0
59.5
1304.3
19.1
581.8
99.2
0.0
3.8
0.0
1060.6
0.0
8.1
0.0
67.2
0.0
0.0
0.0
0.0
0.0
0.0
0.0
9.3
677.1
0.0
127.2
mature
hsa-let-7a
hsa-let-7a
hsa-let-7a
hsa-let-7b
hsa-let-7c
hsa-let-7d
hsa-let-7e
mmu-let-7f
mmu-let-7f
hsa-let-7g
hsa-let-7i
hsa-miR-001
hsa-miR-001
hsa-miR-007
hsa-miR-007
hsa-miR-007
hsa-miR-009
hsa-miR-009
hsa-miR-009
hsa-miR-009*
hsa-miR-009*
hsa-miR-009*
hsa-miR-010a
hsa-miR-015a
hsa-miR-015b
hsa-miR-016
hsa-miR-016
hsa-miR-017-3p
hsa-miR-017-5p
hsa-miR-018
hsa-miR-019a
hsa-miR-019b
hsa-miR-019b
hsa-miR-020
hsa-miR-021
hsa-miR-022
hsa-miR-023a
hsa-miR-023b
hsa-miR-023bN
hsa-miR-024
hsa-miR-025
hsa-miR-026aN
precursor
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-023b-P
MIR-024-1,2
MIR-025
MIR-026a-1,2
Normal tissues
pearson
p-value
-0.101
0.334
0.465
0.735
0.069
0.213
0.683
0.000
0.000
-0.448
-0.100
0.914
0.933
-0.098
0.379
0.434
0.294
1.000
0.992
0.294
1.000
0.992
0.774
-0.320
0.492
0.112
0.116
-0.220
-0.215
0.257
-0.328
-0.325
-0.097
-0.393
0.452
-0.286
0.403
0.395
0.244
0.644
0.189
0.182
0.654
0.128
0.029
< 0.0001
0.759
0.341
0.000
1.000
1.000
0.037
0.656
< 0.0001
< 0.0001
0.664
0.082
0.044
0.184
< 0.0001
< 0.0001
0.184
< 0.0001
< 0.0001
< 0.0001
0.146
0.020
0.620
0.608
0.326
0.336
0.249
0.136
0.140
0.667
0.071
0.035
0.197
0.063
0.069
0.275
0.001
0.399
0.416
Cell lines
pearson
p-value
0.4
-0.125
0.356
0.739
0.963
0.331
0.478
0
0
0.541
0.292
-0.014
-0.041
0.19
0.15
0.64
0.081
-0.008
0.046
0.079
0.077
0.123
0.574
0.403
0.253
0.58
0.297
0.728
0.404
0.754
0.808
0.219
0.131
0.394
0.522
-0.13
0.151
0.256
0.213
0.445
0.479
0.373
0.014
0.462
0.031
< 0.0001
< 0.0001
0.046
0.003
1
1
0.001
0.08
0.936
0.809
0.26
0.375
< 0.0001
0.635
0.961
0.785
0.641
0.649
0.47
0
0.013
0.13
0
0.074
< 0.0001
0.013
< 0.0001
< 0.0001
0.193
0.44
0.016
0.001
0.444
0.372
0.126
0.206
0.006
0.003
0.023
Continued
Table C.5. Correlation between mature and precursor miRNA expression in

Normal tissues and Cell lines.
316
hsa-miR-026b
hsa-miR-026bN
hsa-miR-027a
hsa-miR-027b
hsa-miR-027bN
hsa-miR-028
hsa-miR-029a
hsa-miR-029b
hsa-miR-029c
hsa-miR-030a-3p
hsa-miR-030c
hsa-miR-030d
hsa-miR-030e-3p
hsa-miR-031
hsa-miR-032
hsa-miR-033
hsa-miR-034a
hsa-miR-034aN
hsa-miR-034b
hsa-miR-092N
hsa-miR-092N
hsa-miR-093
hsa-miR-095
hsa-miR-096
hsa-miR-098
hsa-miR-099a
hsa-miR-099b
hsa-miR-100
hsa-miR-101
hsa-miR-101
hsa-miR-103
hsa-miR-105
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-108
hsa-miR-122a
hsa-miR-124a
hsa-miR-125a
hsa-miR-125b
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
hsa-miR-127
hsa-miR-128a
mmu-miR-129-2
MIR-026b
MIR-026b
MIR-027a, b
MIR-027a, b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-029a,c
MIR-030a,e
MIR-030c-1,2
MIR-030d
MIR-030a,e
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034a
MIR-034b
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-126
MIR-127
MIR-128a
MIR-129-2
-0.104
-0.071
0.328
0.058
0.102
0.170
-0.127
0.000
-0.333
0.435
-0.074
0.777
-0.185
0.220
-0.008
-0.088
0.646
0.632
0.268
-0.134
-0.083
0.222
0.282
0.219
0.273
0.081
0.721
0.742
-0.225
0.263
0.179
0.140
-0.035
0.487
0.207
0.000
0.178
1.000
0.884
0.909
0.889
0.470
0.315
0.298
0.720
0.565
0.644
0.753
0.136
0.797
0.653
0.450
0.575
1.000
0.130
0.043
0.744
< 0.0001
0.411
0.325
0.973
0.697
0.001
0.002
0.228
0.552
0.713
0.320
0.204
0.328
0.219
0.719
0.000
< 0.0001
0.315
0.236
0.424
0.534
0.877
0.022
0.355
1.000
0.429
< 0.0001
< 0.0001
< 0.0001
< 0.0001
0.027
0.153
0.177
0.000
0.006
0.116
0.175
0.07
0.159
0.111
0.16
0.774
0.024
0.178
0.547
0.181
0.736
0.515
0.173
0.413
0.419
-0.113
-0.046
0.176
0.757
0.27
0.163
0.33
0.291
0.357
0.258
0.071
0.52
0.342
0.443
0.136
0.08
0.416
0.415
-0.001
0
0
0
0.717
0.395
0.235
0.835
0.806
0.121
0
0.098
0.495
0.299
0.68
0.348
0.512
0.345
< 0.0001
0.887
0.291
0
0.282
< 0.0001
0.001
0.306
0.011
0.01
0.506
0.788
0.298
< 0.0001
0.106
0.336
0.046
0.081
0.03
0.123
0.675
0.001
0.038
0.006
0.421
0.638
0.01
0.011
0.997
1
1
1
< 0.0001
0.016
0.162
< 0.0001
< 0.0001
0.476
1
0.565
Continued
317
hsa-miR-130a
hsa-miR-130aN
hsa-miR-130b
hsa-miR-132
hsa-miR-133a
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
mmu-miR-137
hsa-miR-139
hsa-miR-140
hsa-miR-141
hsa-miR-142-3p
hsa-miR-142-5p
hsa-miR-143
hsa-miR-145
hsa-miR-146b
hsa-miR-147
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-152
hsa-miR-152N
hsa-miR-153
hsa-miR-154
hsa-miR-154*
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-182
hsa-miR-183
hsa-miR-184
hsa-miR-186
hsa-miR-187
hsa-miR-188b
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196a
hsa-miR-196b
MIR-130a-P
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-137*
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-142-P
MIR-143
MIR-145-P
MIR-146
MIR-147
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-152
MIR-152
MIR-153-1,2
MIR-154
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-181a,c
MIR-182
MIR-183
MIR-184
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196a-2
MIR-196b
-0.191
-0.165
-0.152
0.212
-0.021
0.563
-0.051
-0.051
0.991
-0.207
-0.385
0.467
0.126
0.203
0.672
0.327
0.117
0.088
-0.157
0.053
0.932
0.874
0.293
0.229
0.785
0.576
0.732
0.693
0.503
0.282
0.466
-0.013
0.016
0.131
-0.083
-0.147
-0.097
0
-0.39
-0.125
0.359
-0.107
0.002
0.069
0.594
0.911
0.395
0.464
0.500
0.343
0.926
0.006
0.823
0.823
< 0.0001
0.356
0.077
0.028
0.578
0.365
0.001
0.137
0.604
0.696
0.485
0.813
< 0.0001
< 0.0001
0.186
0.305
< 0.0001
0.005
0
0
0.017
0.203
0.029
0.953
0.943
0.561
0.713
0.515
0.668
1
0.072
0.578
0.1
0.636
0.993
0.76
0.004
< 0.0001
0.34
0.294
0.613
0.274
-0.086
0.082
0.028
0.227
0.689
-0.033
0.084
0.667
0.327
0.278
-0.163
0.136
0.09
0
0.135
0.186
0.159
0.413
0.716
0.712
0.417
0
0
0.784
0.099
0.717
0.165
0.239
0.091
-0.065
0.418
-0.016
-0.061
0
0.208
-0.158
0.024
-0.078
0.094
-0.115
0.114
0.192
0.039
0.078
< 0.0001
0.101
0.612
0.631
0.867
0.176
< 0.0001
0.847
0.62
< 0.0001
0.049
0.096
0.335
0.422
0.595
1
0.427
0.269
0.347
0.011
< 0.0001
< 0.0001
0.01
1
1
< 0.0001
0.561
< 0.0001
0.329
0.154
0.594
0.702
0.01
0.924
0.72
1
0.217
0.351
0.888
0.647
0.58
0.499
0.503
0.256
Continued
318
hsa-miR-197
hsa-miR-199a
hsa-miR-199a
hsa-miR-199a*
hsa-miR-199a*
hsa-miR-199b
hsa-miR-200b
hsa-miR-200bN
hsa-miR-200c
hsa-miR-200cN
hsa-miR-203
hsa-miR-204
hsa-miR-205
hsa-miR-206
hsa-miR-208
hsa-miR-210
hsa-miR-210N
hsa-miR-211
hsa-miR-212
hsa-miR-213
hsa-miR-214
hsa-miR-215
hsa-miR-216
hsa-miR-217
hsa-miR-218
hsa-miR-219
hsa-miR-220
hsa-miR-221
hsa-miR-222
hsa-miR-223
mmu-miR-224
hsa-miR-296
hsa-miR-299
hsa-miR-301
hsa-miR-302a
hsa-miR-302a*
hsa-miR-302b
hsa-miR-302c
hsa-miR-302c*
hsa-miR-302d
hsa-miR-320
hsa-miR-321
hsa-miR-323
hsa-miR-324-3p
hsa-miR-324-5p
hsa-miR-325
MIR-197
MIR-199a-1-P
MIR-199a-2-P
MIR-199a-1-P
MIR-199a-2-P
MIR-199b-P
MIR-200b
MIR-200b
MIR-200c
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
MIR-210
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302
MIR-302b
MIR-302c
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-324-P
MIR-325
-0.177
-0.294
0.513
-0.276
0.548
0.728
0.888
0.894
0.412
0.426
0.905
0.915
0.712
0.998
0.976
0.584
0.579
0.204
0.209
0.782
-0.12
0.064
0.911
0
0.705
-0.067
0
0.095
0.364
0.427
0.848
0.324
-0.036
0.311
-0.189
0
-0.023
-0.103
0
-0.109
0.3
0.538
0.663
-0.239
-0.203
-0.044
0.432
0.184
0.015
0.213
0.008
0
< 0.0001
< 0.0001
0.057
0.048
< 0.0001
< 0.0001
0
< 0.0001
< 0.0001
0.004
0.005
0.363
0.352
< 0.0001
0.595
0.779
< 0.0001
1
0
0.769
1
0.674
0.096
0.047
< 0.0001
0.141
0.874
0.158
0.4
1
0.919
0.65
1
0.629
0.175
0.01
0.001
0.285
0.365
0.846
-0.031
0
0
0.052
-0.152
0.093
0.823
0.822
0.378
0.383
0.342
-0.083
0.95
0.02
0
0.205
0.212
-0.048
0.053
0.711
0.015
-0.051
0
0.147
0.083
-0.102
0
0.5
0.253
0.519
0.462
0.097
0
0.565
0.003
0
0.322
-0.011
0
0.101
0.239
0.455
0.343
0.081
0.02
-0.104
0.856
1
1
0.759
0.368
0.585
< 0.0001
< 0.0001
0.021
0.019
0.038
0.625
< 0.0001
0.908
1
0.224
0.208
0.779
0.756
< 0.0001
0.932
0.762
1
0.385
0.626
0.548
1
0.002
0.131
0.001
0.004
0.567
1
0
0.988
1
0.052
0.948
1
0.553
0.154
0.005
0.038
0.633
0.907
0.542
Continued
319
hsa-miR-326
hsa-miR-328
hsa-miR-330
hsa-miR-331
hsa-miR-335
hsa-miR-337
hsa-miR-338
hsa-miR-339
hsa-miR-340
hsa-miR-342
hsa-miR-345
hsa-miR-346
hsa-miR-361
hsa-miR-367
hsa-miR-368
hsa-miR-369
hsa-miR-370
hsa-miR-371
hsa-miR-372
hsa-miR-373
hsa-miR-374
hsa-miR-375
hsa-miR-376a
hsa-miR-377
hsa-miR-378
hsa-miR-379
hsa-miR-380-3p
hsa-miR-380-5p
hsa-miR-381
hsa-miR-382
hsa-miR-383
hsa-miR-384
hsa-miR-422b
hsa-miR-423
mmu-miR-424
hsa-miR-425
hsa-miR-010b
hsa-miR-030b
hsa-miR-138
hsa-miR-151
hsa-miR-200a
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
MIR-010a,b
MIR-030b
MIR-138-1,2
MIR-151
MIR-200a-P
0.608
-0.073
0.852
0.177
-0.151
0.323
0.919
0.413
0.839
0.499
-0.092
0.279
-0.037
-0.213
0.425
0.809
0.722
-0.093
0
-0.092
-0.445
0.617
0.945
0.518
0.772
0.87
0.931
0.674
0.264
0.734
0.862
1
-0.179
0.05
0.431
-0.338
320
0.003
0.747
< 0.0001
0.43
0.503
0.142
< 0.0001
0.056
< 0.0001
0.018
0.682
0.208
0.87
0.341
0.049
< 0.0001
0
0.679
1
0.684
0.038
0.002
< 0.0001
0.014
< 0.0001
< 0.0001
< 0.0001
0.001
0.236
0
< 0.0001
< 0.0001
0.424
0.826
0.045
0.124
-0.161
0.142
0.189
0.349
-0.127
0
-0.136
-0.068
0.591
0.027
0.097
0.057
-0.014
0.089
0
0
0.313
-0.027
-0.005
0.059
-0.263
0.508
0.974
0.064
0.278
0.741
0
0.285
0.116
0.107
0.34
0
0.161
0.412
0.000
0.006
0.334
0
0.104
0
0.286
0.341
0.401
0.262
0.035
0.453
1
0.421
0.69
0
0.875
0.568
0.738
0.935
0.599
1
1
0.059
0.873
0.978
0.73
0.116
0.001
< 0.0001
0.709
0.096
< 0.0001
1
0.087
0.495
0.53
0.04
1
0.34
0.011
1
0.972
0.043
1
0.54
1
0.086
mature
hsa-let-7a
hsa-let-7a
hsa-let-7a
hsa-let-7b
hsa-let-7c
hsa-let-7d
hsa-let-7e
hsa-let-7f
hsa-let-7f
hsa-let-7g
hsa-let-7i
hsa-miR-001
hsa-miR-001
hsa-miR-007
hsa-miR-007
hsa-miR-007
hsa-miR-009
hsa-miR-009
hsa-miR-009
hsa-miR-009*
hsa-miR-009*
hsa-miR-009*
hsa-miR-010a
hsa-miR-010b
hsa-miR-015a
hsa-miR-015b
hsa-miR-016
hsa-miR-016
hsa-miR-017-3p
hsa-miR-017-5p
hsa-miR-018
hsa-miR-019a
hsa-miR-019b
hsa-miR-019b
hsa-miR-020
hsa-miR-021
hsa-miR-022
hsa-miR-023a
hsa-miR-023b
hsa-miR-023bN
hsa-miR-024
hsa-miR-025
precursor
let-7a-1*
let-7a-2-P
let-7a-3*
let-7b*
let-7c*
let-7d*
let-7e*
let-7f-1*
let-7f-2*
let-7g*
let-7i*
MIR-001-1
MIR-001-2
MIR-007-1
MIR-007-2
MIR-007-3
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-009-1-P
MIR-009-2-P
MIR-009-3-P
MIR-010a,b
MIR-010a,b
MIR-015a
MIR-015b
MIR-016-1
MIR-016-2
MIR-017-P
MIR-017-P
MIR-018
MIR-019a-P
MIR-019b-1-P
MIR-019b-2-P
MIR-020
MIR-021
MIR-022-P
MIR-023a
MIR-023b-P
MIR-023b-P
MIR-024-1,2
MIR-025
Pancreatic tissues
pearson
p-value
0.377
0.925
0.696
0.772
0.623
0.859
0.773
0.909
0.648
0.854
0.647
0.044
0.607
-0.122
0.383
0.904
-0.322
0.129
-0.025
-0.444
0.076
-0.3
0.897
0.774
0.871
0.868
0.829
-0.582
-0.255
-0.391
0.879
0.061
-0.54
-0.342
0.213
0.94
0.477
0.959
0.688
0.631
0.972
0.533
0.15
< 0.0001
0.003
0
0.01
< 0.0001
0
< 0.0001
0.007
< 0.0001
0.007
0.871
0.013
0.654
0.143
< 0.0001
0.224
0.633
0.927
0.085
0.78
0.26
< 0.0001
0
< 0.0001
< 0.0001
< 0.0001
0.018
0.341
0.134
< 0.0001
0.823
0.031
0.195
0.429
< 0.0001
0.062
< 0.0001
0.003
0.009
< 0.0001
0.033
Liver tissues
pearson
p-value
-0.18250
-0.25177
-0.05620
0.05478
0.00000
0.51078
0.86061
0.36040
0.00000
0.18968
0.27647
-0.38425
0.41642
0.34160
-0.20180
0.03250
-0.28065
-0.26464
-0.25489
-0.29059
-0.27327
-0.26322
0.04191
0.28917
-0.05809
0.64628
0.71353
-0.15797
-0.23060
-0.27363
0.84429
0.87615
-0.37460
-0.26528
-0.13270
0.84599
-0.05671
-0.41451
-0.01457
0.11443
0.68072
-0.32747
0.24936
0.17344
0.41812
0.42015
0.50000
0.02160
< 0.0001
0.08515
0.50000
0.24084
0.14997
0.07086
0.05431
0.09767
0.22678
0.45245
0.14620
0.16097
0.17037
0.13745
0.15290
0.16232
0.43876
0.13868
0.41539
0.00342
0.00096
0.27950
0.19510
0.15257
< 0.0001
< 0.0001
0.07643
0.16035
0.31209
< 0.0001
0.41738
0.05521
0.47864
0.33652
0.00185
0.10784
Continued
Table C.6. Correlation between mature and precursor miRNA expression in

pancreas tissues and liver tissues.
321
hsa-miR-026aN
hsa-miR-026b
hsa-miR-026bN
hsa-miR-027a
hsa-miR-027b
hsa-miR-027bN
hsa-miR-028
hsa-miR-029a
hsa-miR-029b
hsa-miR-029c
hsa-miR-030a-3p
hsa-miR-030c
hsa-miR-030d
hsa-miR-030e-3p
hsa-miR-031
hsa-miR-032
hsa-miR-033
hsa-miR-034a
hsa-miR-034aN
hsa-miR-034b
hsa-miR-034c
hsa-miR-034cN
hsa-miR-092N
hsa-miR-092N
hsa-miR-093
hsa-miR-095
hsa-miR-096
hsa-miR-098
hsa-miR-099a
hsa-miR-099b
hsa-miR-100
hsa-miR-101
hsa-miR-101
hsa-miR-103
hsa-miR-105
hsa-miR-106a
hsa-miR-106b
hsa-miR-107
hsa-miR-108
hsa-miR-122a
hsa-miR-124a
hsa-miR-125a
hsa-miR-125b
hsa-miR-125b
hsa-miR-126
hsa-miR-126*
MIR-026a-1,2
MIR-026b
MIR-026b
MIR-027a, b
MIR-027a, b
MIR-027a, b
MIR-028
MIR-029a,c
MIR-029b-1,2
MIR-029a,c
MIR-030a,e
MIR-030c-1,2
MIR-030d
MIR-030a,e
MIR-031-P
MIR-032
MIR-033
MIR-034a
MIR-034a
MIR-034b
MIR-034c
MIR-034c
MIR-092-1
MIR-092-2-P
MIR-093
MIR-095
MIR-096-P
MIR-098
MIR-099a
MIR-099b
MIR-100
MIR-101-1
MIR-101-2
MIR-103-1,2
MIR-105-1,2
MIR-106a-P
MIR-106b
MIR-107
MIR-108
MIR-122a
MIR-124a-1,2,3
MIR-125a
MIR-125b-1
MIR-125b-2
MIR-126
MIR-126
0.747
-0.698
-0.693
0.943
0.886
0.845
0.683
0.876
-0.279
0.643
0.224
0.567
0.491
0.354
-0.412
0.451
0.183
0.755
0.727
-0.588
0
0
0.233
-0.39
0.446
0.404
0.298
0.854
0.585
0.386
0.941
-0.184
-0.135
0.914
-0.16
0.239
0.9
0.547
-0.071
-0.217
0
0.595
0.799
0.718
0.821
0.816
0.001
0.003
0.003
< 0.0001
< 0.0001
< 0.0001
0.004
< 0.0001
0.295
0.007
0.405
0.022
0.053
0.178
0.113
0.079
0.498
0.001
0.001
0.017
1
1
0.384
0.135
0.083
0.12
0.261
< 0.0001
0.017
0.14
< 0.0001
0.496
0.619
< 0.0001
0.554
0.372
< 0.0001
0.028
0.793
0.42
1
0.015
0
0.002
< 0.0001
0
-0.10979
-0.20663
-0.19477
-0.50024
-0.23204
-0.27257
0.57878
0.37490
-0.06469
0.12518
-0.51751
-0.28793
0.31596
-0.18962
-0.08562
0.66974
-0.02136
0.13836
-0.14061
-0.12820
0.00000
0.00000
-0.21403
-0.14858
-0.20052
-0.48102
0.24300
0.51564
-0.26063
-0.53726
0.89033
-0.02577
0.68827
0.48609
-0.21435
0.09714
0.79738
0.73696
0.00000
0.50642
0.00000
-0.26899
0.35778
0.00205
0.78681
0.82973
0.34282
0.22130
0.23489
0.02423
0.19358
0.15355
0.00941
0.07625
0.40593
0.32207
0.02003
0.13976
0.11660
0.24092
0.37628
0.00227
0.46872
0.30467
0.30175
0.31805
0.50000
0.50000
0.21303
0.29144
0.22825
0.02963
0.18225
0.02046
0.16480
0.01593
< 0.0001
0.46227
0.00160
0.02813
0.21268
0.36022
0.00011
0.00056
0.50000
0.02266
0.50000
0.15687
0.08682
0.49699
0.00015
< 0.0001
Continued
322
hsa-miR-127
hsa-miR-128a
mmu-miR-129-2
hsa-miR-130a
hsa-miR-130aN
hsa-miR-130b
hsa-miR-132
hsa-miR-133a
hsa-miR-134
hsa-miR-135a
hsa-miR-135b
mmu-miR-137
hsa-miR-139
hsa-miR-140
hsa-miR-141
hsa-miR-142-3p
hsa-miR-142-5p
hsa-miR-143
hsa-miR-145
hsa-miR-146
hsa-miR-146b
hsa-miR-148a
hsa-miR-148b
hsa-miR-149
hsa-miR-150
hsa-miR-152
hsa-miR-153
hsa-miR-154
hsa-miR-154*
hsa-miR-155
hsa-miR-181a
hsa-miR-181b
hsa-miR-181c
hsa-miR-183
hsa-miR-184
hsa-miR-186
hsa-miR-187
hsa-miR-188
hsa-miR-190
hsa-miR-191
hsa-miR-192
hsa-miR-193
hsa-miR-194
hsa-miR-195
hsa-miR-196a
hsa-miR-196b
MIR-127
MIR-128a
MIR-129-2
MIR-130a-P
MIR-130a-P
MIR-130b
MIR-132
MIR-133a-1,2
MIR-134
MIR-135a-1,2
MIR-135b-P
MIR-137*
MIR-139
MIR-140
MIR-141
MIR-142-P
MIR-142-P
MIR-143
MIR-145-P
MIR-146
MIR-146
MIR-148a-P
MIR-148b-P
MIR-149
MIR-150
MIR-152
MIR-153-1,2
MIR-154
MIR-154
MIR-155
MIR-181a,c
MIR-181b-1,2
MIR-181a,c
MIR-183
MIR-184
MIR-186-P
MIR-187
MIR-188b-P
MIR-190
MIR-191
MIR-192
MIR-193
MIR-194-1,2
MIR-195-P
MIR-196a-1
MIR-196b
-0.827
0
-0.281
-0.574
-0.498
0.13
0.843
-0.217
-0.292
0.755
0.852
0.371
-0.024
-0.321
0.43
-0.571
-0.567
0.759
0.939
-0.167
-0.154
0.087
0.679
-0.492
-0.171
0.873
0.483
-0.073
0.276
0.936
0.946
0.311
0.926
0.576
-0.186
0.755
-0.128
-0.008
-0.003
-0.508
-0.4
0.029
-0.436
-0.531
0.214
0.662
< 0.0001
1
0.292
0.02
0.05
0.63
< 0.0001
0.419
0.272
0.001
< 0.0001
0.158
0.93
0.225
0.097
0.021
0.022
0.001
< 0.0001
0.535
0.57
0.75
0.004
0.053
0.525
< 0.0001
0.058
0.789
0.3
< 0.0001
< 0.0001
0.24
< 0.0001
0.019
0.491
0.001
0.637
0.976
0.991
0.045
0.125
0.916
0.091
0.034
0.425
0.005
-0.39102
0.00000
0.00000
-0.33159
-0.32663
-0.14030
-0.00694
-0.41368
-0.19614
-0.21921
-0.30854
0.67248
0.57834
-0.02641
0.05419
-0.01849
-0.16703
-0.10653
-0.12427
-0.10739
-0.27217
-0.13131
-0.02773
0.34013
-0.05861
-0.13685
-0.33352
0.83900
0.89514
0.79543
0.72170
-0.13210
0.66344
0.33014
-0.20374
0.33770
0.37749
0.37749
-0.48602
-0.10806
-0.29203
0.25668
-0.33974
-0.37283
0.13614
0.68659
0.06712
0.50000
0.50000
0.10481
0.10846
0.30214
0.48983
0.05560
0.23329
0.20734
0.12248
0.00216
0.00947
0.46132
0.42101
0.47291
0.26819
0.34728
0.32328
0.34610
0.15392
0.31393
0.45940
0.09870
0.41464
0.30664
0.10341
< 0.0001
< 0.0001
0.00012
0.00080
0.31289
0.00254
0.10586
0.22458
0.10041
0.07473
0.07473
0.02815
0.34519
0.13621
0.16862
0.09897
0.07748
0.30757
0.00165
Continued
323
hsa-miR-197
hsa-miR-199a
hsa-miR-199b
hsa-miR-200a
hsa-miR-200b
hsa-miR-210
hsa-miR-210N
hsa-miR-211
hsa-miR-212
hsa-miR-213
hsa-miR-214
hsa-miR-215
hsa-miR-216
hsa-miR-217
hsa-miR-218
hsa-miR-219
hsa-miR-220
hsa-miR-221
hsa-miR-222
hsa-miR-223
mmu-miR-224
hsa-miR-296
hsa-miR-299-3p
hsa-miR-301
hsa-miR-302a
hsa-miR-302a*
hsa-miR-302b
hsa-miR-302c
hsa-miR-302c*
hsa-miR-302d
hsa-miR-320
hsa-miR-321
hsa-miR-323
hsa-miR-324-3p
hsa-miR-324-5p
hsa-miR-325
hsa-miR-326
hsa-miR-328
hsa-miR-330
hsa-miR-331
hsa-miR-335
hsa-miR-337
hsa-miR-338
hsa-miR-339
hsa-miR-340
hsa-miR-342
MIR-197
MIR-199a-2-P
MIR-199b-P
MIR-200a-P
MIR-200b
MIR-210
MIR-210
MIR-211
MIR-212
MIR-213
MIR-214
MIR-215
MIR-216
MIR-217
MIR-218-1,2
MIR-219-1,2
MIR-220
MIR-221
MIR-222-P
MIR-223
MIR-224
MIR-296
MIR-299-P
MIR-301
MIR-302
MIR-302
MIR-302b
MIR-302c
MIR-302c
MIR-302d
MIR-320
MIR-321
MIR-323
MIR-324-P
MIR-324-P
MIR-325
MIR-326-P
MIR-328
MIR-330
MIR-331-P
MIR-335
MIR-337
MIR-338
MIR-339
MIR-340
MIR-342
-0.508
0.241
-0.559
-0.408
0.36
-0.24
-0.245
-0.289
0.754
0.784
-0.7
0.088
0.176
0.653
0.937
0.543
0
0.871
0.794
0.54
0.574
-0.406
-0.403
0.855
0
0
0.207
-0.382
0
-0.073
-0.129
0.624
0.281
-0.511
-0.253
-0.297
-0.197
-0.423
0.215
-0.31
-0.16
-0.166
-0.472
0.211
0.785
0.278
0.044
0.368
0.024
0.117
0.171
0.37
0.36
0.277
0.001
0
0.003
0.745
0.515
0.006
< 0.0001
0.03
1
< 0.0001
0
0.031
0.02
0.118
0.122
< 0.0001
1
1
0.441
0.144
1
0.787
0.635
0.01
0.292
0.043
0.344
0.264
0.464
0.102
0.424
0.243
0.554
0.539
0.065
0.433
0
0.297
-0.31801
0.50014
0.13411
-0.45630
-0.33746
0.42991
0.44788
0.10396
0.05598
-0.02345
-0.26505
0.16477
0.00000
-0.19435
0.26708
0.48877
0.00000
0.13328
-0.46476
-0.23955
-0.39486
-0.40501
0.09685
0.34673
-0.21158
0.00000
0.48377
-0.13736
0.57278
0.00000
-0.29220
0.36584
0.22682
-0.39547
-0.49403
0.00000
-0.26311
-0.46686
-0.43491
-0.27300
-0.18301
0.00000
-0.30799
-0.35844
0.85806
-0.44967
0.11501
0.02425
0.31024
0.03782
0.10058
0.04826
0.04095
0.35080
0.41843
0.46566
0.16058
0.27099
0.50000
0.23538
0.15866
0.02736
0.50000
0.31134
0.03485
0.18578
0.06506
0.05983
0.36061
0.09414
0.21575
0.50000
0.02881
0.30598
0.01020
0.50000
0.13606
0.08173
0.19912
0.06474
0.02589
0.50000
0.16242
0.03414
0.04614
0.15315
0.24875
0.50000
0.12292
0.08640
< 0.0001
0.04027
Continued
324
hsa-miR-345
hsa-miR-346
hsa-miR-361
hsa-miR-367
hsa-miR-368
hsa-miR-369
hsa-miR-370
hsa-miR-371
hsa-miR-372
hsa-miR-373
hsa-miR-374
hsa-miR-375
hsa-miR-376a
hsa-miR-377
hsa-miR-378
hsa-miR-379
hsa-miR-380-3P
hsa-miR-380-5p
hsa-miR-381
hsa-miR-382
hsa-miR-383
hsa-miR-384
hsa-miR-422b
hsa-miR-423
mmu-miR-424
hsa-miR-425
hsa-miR-020b
hsa-miR-147
hsa-miR-152N
hsa-miR-182*
hsa-miR-196a
hsa-miR-199a
hsa-miR-199a*
hsa-miR-199-s
hsa-miR-199a*
hsa-miR-199-s
hsa-miR-200bN
hsa-miR-200c
hsa-miR-200cN
hsa-miR-203
hsa-miR-204
hsa-miR-205
hsa-miR-206
hsa-miR-208
MIR-345
MIR-346
MIR-361
MIR-367
MIR-368
MIR-369
MIR-370
MIR-371
MIR-372
MIR-373
MIR-374-P
MIR-375
MIR-376
MIR-377
MIR-378
MIR-379
MIR-380
MIR-380
MIR-381
MIR-382
MIR-383
MIR-384
MIR-422b
MIR-423
MIR-424
MIR-425
MIR-020
MIR-147
MIR-152
MIR-182
MIR-196a-2
MIR-199a-1-P
MIR-199a-1-P
MIR-199a-1-P
MIR-199a-2-P
MIR-199a-2-P
MIR-200b
MIR-200c
MIR-200c
MIR-203
MIR-204
MIR-205
MIR-206
MIR-208
-0.599
0.407
-0.588
0.167
-0.278
0.364
0.304
0.347
-0.388
-0.087
0.465
0.56
0.841
0.464
-0.488
0.792
-0.055
0.477
-0.129
0.096
0.078
0
-0.547
-0.565
0
0.444
0.014
0.117
0.017
0.536
0.297
0.166
0.252
0.188
0.137
0.749
0.07
0.024
< 0.0001
0.07
0.055
0
0.839
0.062
0.634
0.725
0.773
1
0.028
0.022
1
0.085
0.389
-0.027
-0.023
0.646
-0.281
0.903
0.268
0
0.136
0.92
0.933
0.007
0.292
< 0.0001
0.316
1
325
-0.33898
0.04167
-0.36522
-0.32884
0.28113
0.35733
0.95425
0.04957
-0.23649
-0.14928
0.41782
0.19890
0.99230
-0.18468
0.17028
0.99176
0.98244
0.43793
-0.26214
-0.00827
-0.06868
0.00000
0.28046
-0.53373
0.00000
-0.02134
0.12952
-0.02312
-0.12697
0.00336
0.17554
-0.49145
-0.52976
-0.51851
0.43724
0.46991
0.09951
0.43911
0.08212
0.10683
0.14576
0.08711
< 0.0001
0.42767
0.18894
0.29055
0.05366
0.23011
< 0.0001
0.24676
0.26419
< 0.0001
< 0.0001
0.04489
0.16335
0.48788
0.40023
0.50000
0.14636
0.01661
0.50000
0.46874
0.31630
0.46613
0.31969
0.49508
0.25776
0.02660
0.01741
0.01981
0.04517
0.03313

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EXPRESSION AND FUNCTION OF MICRORNA IN HUMAN CANCER

Presented in Partial Fulfillment of the Requirements for the

The Ohio State University

Dr. James T. Dalton

microRNAs (miRNA) are functional 22 nt noncoding RNAs that negatively

I would like to express sincere appreciation and gratitude to my advisor, Dr.

Christine Esau for support of antisense oligonucleotides and expertise on antisense

November 29, 1975... Born in Ulsan, South Korea

inhibitor, DA-8159, using an equilibrium dialysis technique. Biopharmaceutics &

2. EXPRESSION PROFILING IDENTIFIES MICRORNA SIGNATURE IN

3. ANTISENSE INHIBITION OF microRNA REDUCES CELL VIABILITY

3.4 DISCUSSION .................................................................................................62

Clinical data and tumor pathology ........................................................................46

Top 20 aberrantly expressed miRNA precursors in pancreatic adenocarcinoma .47

Sequence and chemical modifications to antisense oligonucleotides ...................73

Antiproliferative activity parameters of ASO against miR-21, -100, -212, -221

microRNA that display tissue-specific or tissue enriched expression ................115

Relative expression levels of mature miRNA and miRNA forms ....................116

miRNA biogenesis ................................................................................................19

Chemical modification of antisense oligonucleotides ..........................................20

18S rRNA expression in pancreatic tissue ............................................................38

miRNA precursor expression in pancreatic samples ............................................39

Three dimensional expression terrain map ...........................................................40

Estimated probabilities for the training and test data ............................................41

miRNA expression by Northern blotting ..............................................................42

Validation of precursor and mature miRNA levels ..............................................44

Histologic and molecular analysis of pancreatic cancer for microRNA

Structures of gemcitabine and deoxycytidine .......................................................65

Comparison of three antisense oligonucleotides ...................................................66

The effect of antisense oligonucleotide and control oligonucleotide in pancreatic

Effect of control oligonucleotides on cell proliferation ........................................69

ASO pretreatment sensitized the antiproliferative effects of gemcitabine ...........71

Flow cytometric analysis of the effect of antisense oligonucleotide (ASO) on cell

Flow cytometric analysis of apoptosis in HS766T cells .......................................93

Target validation by luciferase assay and FOXO3a western blotting ...................96

microRNAs with good correlation in expression levels of precursor and

Expression of miR-128a in 22 normal tissues and cells .....................................111

Lack of microRNA processing in cancer cell lines ............................................112

miR-31 expression in cancer cell lines ...............................................................113

Pearson correlation among the various samples studied .....................................114

Pancreatic cancer progression model and genetic alteration .............................123

1.1 miRNA DISCOVERY

The lin-4 gene

produced ~ 22 nt noncoding RNA that binds to sequences in the 3 untranslated region

The interaction between lin-4 and lin-14 caused

They discovered that double-stranded RNA (dsRNA)

sequence-specific mRNA degradation during RNA interefence (RNAi).

1.2 miRNA BIOGENESIS

Human miRNAs are distributed in all chromosomes except the Y chromosome.

Most miRNAs are transcribed by RNA polymerase II.

Argonaute protein (Ago 2) to form the RNA-induced silencing complex (RISC)

One control mechanism is

transcriptional regulation. let-7 is regulated by a transcriptional enhancer element in C.

Another mechanism is post-transcriptional regulation.

1.3 miRNA TARGET IDENTIFICATION

miRNAs control gene expression by binding to the 3 UTR of target mRNA. It is

Further studies (46, 47)

If the 5 sequence has weaker binding, then the 3

compensatory binding is required. However, extensive binding at the 3 site without 5

complementarity is not enough to have miRNA function (46).

complementary sequence is important to predict the target gene, 3 complementary site is

(http://www.targetscan.org) (45, 47, 51) is one program used to predict mammalian

(http://pictar.bio.nyu.edu) (52-54) is used to identify miRNA target genes in C. elegans,