SRM UNIVERSITY DEPARTMENT OF BIOTECHNOLOGY SCHOOL OF BIOENGINEERING Subject: FERMENTATION ENGINEERING Subject code: BT0522 LESSON PLAN S.No.

Lecture Hours Topic / Content UNIT I Fermentation Industry 1 2 3 4 5 6 7 8 9 10 1 1 1 1 1 1 1 1 1 1 Overview of fermentation industry Requirements of Fermentation processes Energetic of microbial metabolites Types of fermenter - Classifications Basic configuration of fermenter Agitators Agitators and impellers- Types Physical and chemical parameters that affect the fermentation process Instrumentation in fermenter Control parameters in fermenter Computer Aided control in the fermenter UNIT II Media Formulation Media Formulation Preparation of Complex and synthetic media Preparation of natural media Preparation of synthetic media Technological concerns in Media Design Engg. Selection of components Stoichiometric calculations Batch and continuous heat sterilization of liquid media Application - Design of continuous sterilizer and media Filter sterilization of liquid media Air sterilization Thermal death kinetic studies Thermal death kinetic calculations 1&4 1&4 1&4 1 &4 1&4 2&4 2&4 2&4 2&4 2&4 Book Learning outcomes Semester: II Year: 2011

To learn about the fermentation industry and the fermenters and its components and their controls

11 12 13 14 15 16 17 18 19 20

1 1 1 1 1 1 1 1 1 1

1&7 1&7 7 1&7 5&6 3,4 &5 3,4 3,4 5,6 To learn about the formulation of media, stoichiometric calculations and sterilization kinetics

5,6 UNIT III Inoculum Development & Fermentation 1,7&8 1,7&8 1,7&8 1,7&8 1,7&8 To learn isolation, purification and maintenance of culture and kinetics study of batch, continuous and fed batch culture.

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Isolation of cultures Selection of culture Improvement of cultures-Screening methods and Downstream Processing Culture preservation, Strain improvement Aseptic culture transfer and incubation Inoculum age, size and studies on growth kinetics

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Kinetics of Batch and continuous culture Kinetics of Fed batch culture Industrial manufacture of important biotechnological products

1,7&8 1,7&8 1,7&8

UNIT IV CONSTRUCTION OF BIOREACTORS 29 30 31 32 33 34 35 36 37 38 1 1 1 1 1 1 1 1 1 1 Types of bioreactor configuration Mass transfer analysis of bioreactors Materials of construction for fermentation processes Design and Analysis of bioreactors Oxygen mass transfer in fermentation process Methods for the determination of mass transfer coefficients Scale up criteria for bioreactors based on oxygen transfer Power consumption and impeller tip speed Problems Types of aerators, importance of aeration in fermentation Modes of reactor operation UNIT V Application of Fermentation Technology 39 1 Manufacture of antibiotics Production of penicillin,Streptomycin, Tetracycline 1,2 &4 etc., Solvents 1,2 &4 Ethanol production Acetone, Butanol production 1,2 &4 Production of Amino acids and enzymes (Amylase, Protease) Organic acids (Citric acid) Bio fertilizers, Bio pesticides Biopharmaceuticals 1,2 &4 1,2 &4 1,2 &4 1,2 &4 4 4 3 5 4 4 4 4 4 4 To learn how to construct the bioreactor, analysis of design, Scale up criteria and Heat and Mass transfer methods

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1 1 1 1 1 1

To learn about the production of primary and secondary metabolites in fermentation industries and their applications

References 1. Bailey J.E. and Ollis, D.F. Biochemical Engineering Fundamentals, McGraw Hill, 2. Michael L Shuler and Kargi, Bioprocess engineering, Basic concepts, Prentice Hall, (2004). 3.Biochemical Engineering by James M.Lee , Prentice Hall 1992. 4. Aiba .S., et al Biochemical Engineering, Academic press, London, 1965 5. Pauline M. Doran Bioprocess Engineering Principles, Academic press, 1995 6. Harvey W. Blanch and Douglas S. Clark, Biochemical Engineering, Marcel Dekker (1986). Second

Indian

Reprint

Inc. (1997)

7. Scragg. A.H., Bioreactors in Biotechnology A Practical approach:1991 (1993). Subject in Charge: 1. Mrs. M. PANDIMA DEVI 2. Mr. M. VENKATESH PRABHU 3.Miss M. RAMYAPRIYA 9444145987 9894313473 9865446062 pandimadevim@ktr.srmuniv.ac.in venkateshprabhum@ktr.srmuniv.ac.in ramsbiotech@gmail.com