Subject: Quotation for Trials for Sugar Cane Juice Sample Some points from J P Uncle: 1.

Experts have claimed that getting a shelf-life of 6 months for Sugarcane juice is a very difficult task, and yet not established. At least not commercially 2. Practically, a shelf-life of 2.5-3 months may be possible 3. Use of Nanotechnology and Irradiation for extending shelf-life are the latest developments, but yet not a preferred options. 4. Side effects of the methods are yet not assessed (similar to the conservative approach against GM foods) 5. Citric acid is the best option to be the preservative. Even in comparison with Ascorbic Acid 6. Potassium meta-bisulphite needs to be checked again (fear of its poisonous effects and legislative norms). Sodium meta-bisulphite could be an option instead. 7. PET bottles are not an option at all for preserved Sugarcane juice. 8. Tetra Pak could be an option, but time-temperature regime needs to be redesigned to suit the juice.

Rough Estimate of Costs involved 1. Sugarcane Juice: a. 48 Litres b. Rate: Rs. 10 per 200 ml c. Total: Rs. 2500 2. Citric a. b. c. Acid: 10 g Rate: Rs 2000 per 150 g Total: Rs 200

3. Sodium Meta-bisulphite: a. 5 g b. Rate: Rs 500 per 400 g c. Total: Rs 20 4. Sample size: a. No. Of Intervals: 8 (0, 10, 20, 30, 45, 60, 75 and 90 Days) b. No. Of Samples per interval: 12 c. Total no. Of Samples: 96

Costing Table: Requirement Sugar Cane Juice Citric Acid Sodium Meta-bisulphite Stand-Up Pouches Labels Muslin Cloth pH meter Brix Refractometer (0-30) Water-Bath Thermometer Weighing balance Refrigeration Box / Cooler Grand Total TUV Lab Quotation
Physio-Chemical Tests Uni t Brix Reduci ng Sugars (%) Microbiologi cal Tests Yeast and Mould Sensory Tests Total (96 Samples )

Total 48000 10000 5000 72 72 24 1 1 1 1 1 1

Unit ml mg mg

Cost 2500 200 20 2000 150 50 1500 2000 7000 400 13100

Particu lar (Per Sample )

Sug ars (%)

Acidit y (%)

pH

Viscosi ty

TPC

Visu al

Flavo ur

Overa ll

Sample Volume Volume for Retentio n Lead Time Cost

ml

250 ml

24 litres

ml

250 ml

24 litres 5 days 3 lakhs

INR

200

600

400

250

200

800

300

400

GeoChem Lab Quotation a. b. c. d. Availability of cold storage for Samples Viscosity and Brix measurement at 30 C (temperature) Lab Tests Rs. 4.2 Lakhs Overall Costing including storage: Rs. 4.5 lakhs
Physio-Chemical Tests Uni t Brix Reduci ng Sugars (%) Microbiologi cal Tests Yeast and Mould Sensory Tests Total (96 Samples )

Particu lar (Per Sample )

Sug ars (%)

Acidit y (%)

pH

Viscosi ty

TPC

Visu al

Flavo ur

Overa ll

Sample Volume Volume for Retentio n Lead Time Cost

ml

250 ml

24 litres

ml

250 ml

24 litres 5 days

INR

200

600

400

250

200

800

300

400

4.2 lakhs

Overall Estimated Costs for 3 month Formulation Trial: 1. Material: Rs. 13,100 2. GeoChem Lab tests: Rs. 4,50,000 3. Grand Total: Rs. 4,65,000