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David Sojka, Ph.D. ds1260b70@westpost.net * (505) 336-0505 1077 S. Taylor Ave., St.

Louis, MO 63110, USA Prievidzska 2, 78701, Sumperk, Czech Republic REGULATORY AFFAIRS: EDUCATION AND EXPERIENCE o 2011 University of California San Diego Extension Enrolled in Professional Certificate in Regulatory Affairs (FDA regulatory requirements and compliance) o Theoretical knowledge of the GCP, GMP and GLP guidelines o 2011 BentleyCzech (Bentley Instruments Inc., MN, USA) My position is to serve as intermediary between the regulatory agencies in Russia, the Czech and Slovak Republics and companies based in Western Europe and New Zealand to get approval of their products in Central and Eastern European countries. Other work experience: o 2008 Assistant of the organizer of the European MYORES convention o 2004-05 Computer network administrator, Charles University o 2004-05 Administrative work at Mediatel, Prague, CR o 2003-04 Assistant; www.miga.cz start-up company EDUCATION-SCIENCE 2010-11 WASHINGTON UNIV. ST. LOUIS SCHOOL OF MEDICINE Postdoctoral Research Associate Publications: second author of a paper in demonstrating a novel mechanism of synaptic transmission impairment in a model of mental retardation. I introduced and applied Ca imaging technique for measurement of Ca dynamics in single synaptic terminals in culture 2009 Ph.D. in Physiology, Pain Research (Inst. of Physiology, Academy of Sciences, Prague, Czech Republic 2005 M.Sc. in Physical chemistry (Charles University, Prague) EDUCATION-ECONOMICS 2003-09 University of Economics, Prague, Czech Republic Faculty of Business Administration Selected subjects: Applied Marketing, Advanced Business Administration, Financial Analysis, Microeconomics, Macroeconomics, Management & Cost Accounting, Business Innovations AWARDS o EMBL Training Travel Grant, Germany (2005) 20 PhDs and postdocs out of 500 candidates were selected based on quality of their research proposal o Award for oral research presentation for young investigators at Conference of the Czech and Slovak Physiological Society, Prague, CR (2009) SCIENTIFIC TRAINING o EMBL, Heidelberg, Germany: Microarray. o Palm Microlaser, Bernried, Germany: Laser Microdissection. o Institute of Hematology and Blood Transfusion, Prague: qRT-PCR

o Charles University, Prague, CR: Methods in Pharmacology SKILLS o Highly motivated self-starter who takes initiative with minimal supervision. o Eager to meet challenges and quickly assimilate new concepts. o Highly analytical thinking with demonstrated talent for identifying, scrutinizing, improving, and streamlining complex work processes o Results-driven achiever with exemplary planning and organizational skills, along with a high degree of detail orientation. o Proven relationship-builder and team-player with unsurpassed interpersonal skills. LANGUAGES o Czech (fluent), Spanish, German (intermediate) EXPERIMENTAL TECHNIQUES o behavioral (application of local anesthetics in a model of surgical pain) o histological (intracardial perfusion, spinal cord dissection, cryosectioning) o proteomics (Western blot, low-molecular weight protein electrophoresis, 2D-electrophoresis, dialysis, enzymatic cleavage of glycoproteins) o spectroscopy (fluorescence spectroscopy, TPDS, circular dichroism) o primary cell cultures o imaging (calcium imaging on tissue slices and neurons in culture, in vivo calcium i maging, super-resolution microscopy and measurement of synaptic vesicle dynamics o basic practical experience with patch clamp technique COMPUTER LITERACY o Extensive software proficiency covering wide variety of applications. o Data and Image Analysis: Matlab, Origin, Excel, ImageJ, AIDA. o Statistics: Statgraphics, Statistica, SPSS. o Linux OS, Windows OS, Python programming language. __________________________________________________________ Publications: 1. Changes of Calcium Binding Protein Expression in Spinothalamic Tract Neurons After Peripheral Inflammation. Sojka D. - Zacharova G. - Spicarova D. - Palecek J. (Physiol Res, 59, 2010) 2. The Effect of Unilateral Arthritis on Parvalbumin Expression in Dorsal Horn and Dorsal Root Ganglion Neurons. Zacharova G. - Sojka D. - Palecek J. (Physiol Res, 58, 2009) Submitted: 3. Intracellular Calcium Changes Induced in Spinal Cord Dorsal Horn Neurons by Dorsal Root Stimulation. Sojka D. - Palecek J. (Molecular Pain, submitted 2010) In preparation: 4. In Vitro Model of Central Sensitization. Sojka D. - Palecek J. 5. Second author of a paper from my current postdoc position. Conventions: Oral presentations: 2005 EMBL Microarray Training, Heidelberg, Germany

2005, 2009 Annual Meeting of the Czech and Slovak Physiological Soc. Poster presentations: 2006, 2007, 2008 Annual Meeting of The Society for Neuroscience, USA 2006 FENS Forum Vienna, Austria

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