Significant association of high-risk human papillomavirus (HPV) but not of p53 polymorphisms with oral squamous cell carcinomas

in Malaysia
Article type: Article Abstract: Purpose: The purpose of this study was to evaluate the role of HPV and p53 polymorphisms in oral squamous cell carcinomas (OSCC) affecting Malaysian population. Methods: We analysed frozen samples from 105 OSCC as well as 105 oral specimens derived from healthy individuals. PCR assays targeting two regions of the virus were used. PCR amplification for the analysis of p53 codon 72 arginine/proline alleles was carried out in a separate reaction. Results: HPV DNA was detected in 51.4% OSCC samples, while 24.8% controls were found to be HPV positive. HPV was found to be significantly associated with OSCC (P < 0.001, OR = 4.3 after adjustment for habits) when compared to controls. High-risk HPV was found to be significantly associated with OSCC cases (P < 0.05). Demographic profiles of age, gender, race and habits were not associated with HPV presence in cases and controls. However, significantly less HPV positivity was seen in poorly differentiated compared to welldifferentiated OSCCs. No significant association was found between HPV positivity and p53 polymorphisms in cases and control groups. Additionally, we found no association of codon 72 polymorphism with oral cancer. Conclusions: This study indicates that high-risk HPV infection is one of the contributing factors for OSCCs. HPV 16 was the predominant type found in Malaysian patients with OSCC. Further, we did not find any association between p53 codon 72 polymorphism and HPV infection or between the p53 polymorphism and the risk of oral cancer. Author Saini, R. ; Tang, T. H. ; Zain, R. B. ; Cheong, S. C. ; Musa, K. I. Saini, D. ; Ismail, A. R. ; Abraham, M. T. ; Mustafa, W. M. W. Santhanam, J. Journal of Cancer Research and Clinical Oncology 0171-5216 10.1007/s00432-010-0886-8 137 311-320 2011

Source ISSN DOI Volume Page Year Keyword:

High-risk HPV, Oral squamous cell carcinoma, p53 polymorphism, Oral squamous cell carcinoma, OSCC, lichenoid lesions, lichen planus, oral cancer, oral tumours, pemphigus, traumatic eosinophilic granuloma, aphthous ulcers, oral mucosal lesions, betel chewers mucosa, betel quid related lesions, betel quid, areca quid, tobacco quid, oral cancer screening, training and calibration, early detection, oral cancer awareness,

biobanking, tissue bank, databank, oral cancer, tissue bank, research credibility, research ethics. Please Cite As: SAINI, R., TANG, T. H., ZAIN, R. B., CHEONG, S. C., MUSA, K. I., SAINI, D., ISMAIL, A. R., ABRAHAM, M. T., MUSTAFA, W. M. W. & SANTHANAM, J. 2011. Significant association of high-risk human papillomavirus (HPV) but not of p53 polymorphisms with oral squamous cell carcinomas in Malaysia. Journal of Cancer Research and Clinical Oncology, 137, 311-320. URL: http://www.scopus.com/inward/record.url?eid=2-s2.078751610603&partnerID=40&md5=f40ad8420808cc434bb31b27a7d48a94 http://www.springerlink.com/content/u07501t1n28x2857/ http://cat.inist.fr/?aModele=afficheN&cpsidt=23596605 http://www.ncbi.nlm.nih.gov/pubmed/20419384