Orochem Technologies Inc.

Our History Orochem Technologies Inc., established in 1996, is a biotech company that serves the bioanalysis, genomics, drug discovery, and the proteomics markets. Orochem Technologies Inc. is dedicated to the development of value-added medical devices that feature unique components, custom surfaces, specialized membranes, and highly selective solid phase sorbents integrated into formats that enable high throughput sample preparation and screening. Moving beyond its established reputation as the pre-eminent supplier of sample preparation tools, the acquisition of Column Engineering Inc in 2006 provided an opportunity for us to service the varied HPLC market. Since then Orochem Technologies has greatly broadened its expertise and manufacturing capabilities in HPLC products and services. From Silica synthesis to specialized bonded phase development, Orochem products service the analytical, semi-prep and preparative needs of pharmaceutical products, contract research, API development and the genomic and proteomics applications. Our goal is to provide the best value to our customers, innovation in integrated processes for analytical and prep chromatography, high quality, low cost, and prompt services. Discover the benefits of dealing directly with the column experts contact us today!

Content

HPLC COLUMNS

Our History Mission Statem ent Ordering Inform ation HPLC Flow Chart Checking a Catalog Item EpitomizeTM Chiral Column GazelleTM 1.7um UHPLC Colum n Matrix TM HPLC Colum n Monitor TM HPLC Colum n Prestige TM HPLC Column Reliachrom TM ReliasilTM HPLC Column OroPureTM HPLC Colum n OroSym TM HPLC Column OroSilTM HPLC Column OroStar TM HPLC Column Nuclesil Guard Cartridges Prep/Sem i-Prep Column Test Mix Orochem Colum ns Cross Referenced w ith Industry Columns Industry Columns Cross Referenced with Orochem Columns Contact Inform ation

1 3 4 5 6 7 10 11 14 18 20 22 26 27 33 35 36 37 44 45 49 54 61

Orochem Technologies Inc.

Mission Statement At Orochem Technologies, the core expertise is in the development and manufacture of customized individual and high throughput sample preparation devices, unique chemistries for columns, analytical, preparatory, and chiral chromatography. We are driven to understand and offer tools for parallel processing, scale-up and process chromatography. Our mission is to offer a one-shop to integrate indigenously developed chemistries for separations into custom plastic molded or machined devices. Knowledge of chemical biochemical processes assay sample preparation system allows us to integrate and support sample prep and chromatography systems into automated robotic workstations.

Ordering Information

MAIL TO:

Orochem Technologies Inc. 331 Eisenhower Ln S Lombard, IL 60148 Tel.: 630.916.0225 Fax: 630.916.0250 Email: asha@orochem.c om Web: www. orochem.com

HPLC COLUMNS

PRICE: SHIPPING: WARRANTY:

Quoted in U.S . Dollars and subject to change without notice UPS Ground or FedE x unless otherwis e specified All products are warranted t o be free from defects in materials and workmanship.

TERMS: RETURNS:

FOB point of shipment, Net 30 days on approval of credit. All returns are subject to 15% re-stoc king f ee. For any damaged goods, OROCHEM TECHNOLOGIES, INC. must be informed within 7 days to receive proper credit or replacement with a R eturn Godds Authorization. Goods must be in their original pac kaging to obtain proper credit. Due t o the nature of pac kaging however, some goods may not be returnable.

Orochem Technologies Inc.

HPLC FLow Chart

SOLUBILITY

MODE

PHASE
Amino, Cyano RELIASIL Silica

PAGE

Organic-soluble only

NORMAL PHASE

RELIASIL, MONITOR

14, 22

Soluble in H2O or organic C18, C8, etc.,

MW < 2000
Aqueous-soluble only

REVERSE PHASE CHROMATOGRAPHY

RELIASIL, MONITOR, MATRIX Phenyl Cyano

11,14,

Strong Cation or Anion Exchanger Aqueous-soluble only

ION-EXCHANGE

RELIASIL

22

Checking a Catalog Item

Check your desired product in the ordering information section Ordering information

Column Size
Phase 3 m Silica 5 m C18 50 x 2.0 mm R3BE-100 R5BE-121 50 x 4.6 mm R3BI-100 R5BI-121

Particle Size

HPLC COLUMNS

Packing Material Type R:Reliasil M: Monitor

Part Number

Orochem Technologies Inc.

EpitomizeTM Chiral CSP1 Columns
EpitomizeTM CSP1 Chiral columns are based on cellulose tris (3,5-dimethylphenylcarbamate) coated on 5um spherical silica with 10um and 20um available upon special request. Typical Applications: Four applications have been chosen to show the excellent chiral separation selectivity of the EpitomizeTM CSP1 material when compared to a popular competitive material. The EpitomizeTM CSP1 Chiral material used for these comparisons was a 10um material and the examples show excellent separations and efficiencies even when compared to the 5um High Efficiency version from the competition. The applications selected are 1) The separation of the isomers of Atropine 2) The separation of the isomers of Proglumide 3) The separation of the isomers of Flavanone 4) The separation of the isomers of Homatropine

Atropine on 5um competitive material

Column Conditions for Atropine Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) Flow Rate: 1.0mL/min Injection Volume: 10um

EpitomizeTMChiral Column

Proglumide on 5um competitive material

HPLC COLUMNS

Column Conditions for Proglumide Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) + 0.1% TFA Flow Rate: 1.0mL/min Injection Volume: 10um

Flavanone on 5um competitive material

Column Conditions for Flavanone Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) Flow Rate: 1.0mL/min Injection Volume: 10um

Orochem Technologies Inc.

Homatropine on 5um competitive materia

Column Conditions for Homatropine Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) Flow Rate: 1.0mL/min Injection Volume: 10um

Ordering Information
Popular Dimensions
Phase Epitomize CSP1 50x2.0 E5BE-927 50x4.6 E5BI-927 150x4.6 E5FI-927 250x 4.6 E5HI- 927

Unique sizes available

Phase Epitomize CSP1

30 x 4.6 mm E5AI-927

100 x 4.6 mm E5DI-927

30 x 3 mm E5AG-927

50 x 3 mm E5BG-927

Phase Epitomize CSP1

100 x 3 mm E5DG-927

125 x 3.0 mm E5EG-927

150 x 3 mm E5FG-927

150 x 2 mm E5FE-927

250 x 3 mm E5HG-927

300 x 3.9 mm E5IS-927

GazelleTM 1.7um UHPLC Column

GazelleTM 1.7um UHPLC Column

GazelleTM is designed by Orochem Technologies, especially to yield higher sensitivity and reproducibility in less run time. Orochem uses a proprietary silica type A manufacturing process that yields a narrow particle size and pore size distribution, which gives you consistent and reproducible results. The hydrophobic spherical surface is also stable over a wide pH range (2-8) to allow for excellent method flexibility. Advantages: Good Resolution Faster Analysis High Sensitivity Low band spreading Saves Solvent

HPLC COLUMNS

Features: Surface Area: 190m2/g Pore Size: 100A Pore Volume: 0.6 pH Range: 2-8 Particle Size: 1.7um

Retention of Non-Polar Compounds

Column: Gazelle 1.7um C18 50 x 2.1 mm Mobile Phase: 60% Acetonitrile/40% Water

Temperature: 30 oC Flow Rate: 0.2 ml/min Detection: 254uv

0.5 1.2 1.7 2.9 3.8

Test Mix 1. Uracil 2. Acetophenone 3. Methyl Benzoate 4. Toluene 5. Naphthalene

Ordering Information
Phase Silica C 18 50 x 2.1 mm G17BQ-930 G17BQ-931 30 x 2.1 mm G17AQ-930 G17AQ-931

10

Orochem Technologies Inc.

Matrix TM HPLC Column
MatrixTM C18 MatrixTM uses base-deactivated ultra-high purity silica, densely bonded to produce an inert material ideally suited for the analysis of basic compounds. The resulting hydrophobic surface is also stable over a wide pH range (2-9) to allow for excellent method flexibility. MatrixTM C18 AQ MatrixTM C18 AQ has been specifically designed for the analysis of polar analytes in high aqueous mobile phases. Matrix TM C18 AQ features the traditional C18 ligand interspersed with hydrophilic end-capping groups. The resulting material is easily wettable, and unlike conventional C18 materials, does not exhibit retention loss in high aqueous conditions.

Surface Area: 300m2/g Pore Size: 100A Pore Volume: 0.95 pH Range: 2-8 Particle Size: 3um, 5um, 10um

Matrix C18 250 x 4.6 mm Mobile Phase: 20% Acetonitrile 80% 0.05M Phosphate Buffer, pH 2.5 Flow Rate: 1.0 mL/min Sample: 1. Pseudoephedrine 2. Lidocaine 3. Pyrilamine 4. Chlorpheniramine

11

MatrixTM HPLC Column

Mobile Phase: 69% Water, 28% MeOH, 3%Acetonitrile Flow Rate: 1.2 mL/min Sample: 1. Acetaminophen 2. Caffeine 3. Aspirin 4. Benzoic Acid

HPLC COLUMNS

Benefits: 1. Ultra-High Purity Silica 2. AQ-C18 Version for high-aqueous conditions 3. Excellent value 4. pH-stable from 2-9 5. Custom Column Geometries available

Ordering Information
Popular Dimensions
Phase 3 m NH2 3 m C8 3 m AQ 3 m C18 50 x 2.0 mm R3BE - 138 R3BE - 135 R3BE - 171 R3BE - 136 50 x 4.6 mm R3BI - 138 R3BI - 135 R3BI - 171 R3BI - 136 150 x 4.6 mm R3FI - 138 R3FI - 135 R3FI - 171 R3FI - 136

Phase 5 m NH2 5 m C8 5 m C18 AQ 5 m C18

50 x 2.0 mm R5BE - 137 R5BE - 130 R5BE - 170 R5BE - 131

50 x 4.6 mm R5BI - 137 R5BI - 130 R5BI - 170 R5BI - 131

150 x 4.6 mm R5FI - 137 R5FI - 130 R5FI - 170 R5FI - 131

250 x 4.6 mm R5HI-137 R5HI-130 R5HI-170 R5HI-131

12

Orochem Technologies Inc.

Unique size available:

Phase 3 m NH2 3 m C8 3 m AQ 3 m C18 30 x 4.6 mm R3AI-1 38 R3AI-1 35 R3AI-1 71 R3AI-1 36 100 x 4.6 mm R3DI-1 38 R3DI-1 35 R3DI-1 71 R3DI-1 36 150 x 2 mm R3FE-138 R3FE-135 R3FE-171 R3FE-136

Phase 3 m NH2 3 m C8 3 m AQ 3 m C18

30 x 3 mm R3AG-138 R3AG-135 R3AG-171 R3AG-136

50 x 3 mm R3BG-138 R3BG-135 R3BG-171 R3BG-136

100 x 3 mm R3DG-138 R3DG-135 R3DG-171 R3DG-136

125 x 3.0 mm R3EG-138 R3EG-135 R3EG-171 R3EG-136

150 x 3 m m R3FG-138 R3FG-135 R3FG-171 R3FG-136

250 x 3 m m R3HG-138 R3HG-135 R3HG-171 R3HG-136

300 x 3.9 mm R3IS-138 R3IS-135 R3IS-171 R3IS-136

Phase 5 m NH2 5 m C8 5 m C18 AQ 5 m C18

30 x 4.6 mm R5AI-137 R5AI-130 R5AI-170 R5AI-131

100 x 4.6 mm R5DI-137 R5DI-130 R5DI-170 R5DI-131

150 x 2 mm R5FE-137 R5FE-130 R5FE-170 R5FE-131

Phase 5 m NH2 5 m C8 5 m C18 AQ 5 m C18

30 x 3 mm R5AG-137 R5AG-130 R5AG-170 R5AG-131

50 x 3 mm R5BG-1 37 R5BG-1 30 R5BG-1 70 R5BG-1 31

100 x 3 mm R5DG-137 R5DG-130 R5DG-170 R5DG-131

125 x 3.0 mm R5EG-1 37 R5EG-1 30 R5EG-1 70 R5EG-1 31

150 x 3 mm R5FG-137 R5FG-130 R5FG-170 R5FG-131

250 x 3 mm R5HG-137 R5HG-130 R5HG-170 R5HG-131

300 x 3.9 mm R5IS-1 37 R5IS-1 30 R5IS-1 70 R5IS-1 31

13

MonitorTM HPLC Column

MonitorTM HPLC Column

MonitorTM is a top-quality phase, providing excellent stability and reproducibility, and minimal secondary interaction. Monitor is a perfect choice for reversed phase separations requiring thoroughly base-deactivated columns, and excels in challenging pharmaceutical and peptide separations. We developed MonitorTM expressly for the chromatography of difficult-to-separate samples. The spherical base silica is made using a two-step hydrolysis of high-purity TEOS under tightly controlled conditions. By using only high purity reactants to manufacture the base gel, excellent base-deactivation characteristics are achieved, and our reaction conditions are optimized to yield smooth pores with a narrow pore size distribution. Elimination of fines in the classification step results in columns of exceptionally low back pressure. Monitor columns have unsurpassed reduced surface activity, and excel in the separation of basic compounds, peptides, and amino acids. Manufactured by Orochem Technologies Inc., using state-of-the-art technology; for reversed phase separations with minimal secondary interactions, Monitor Columns are an excellent alternative for most C18 applications requiring base-deactivated columns, especially for difficult pharmaceutical and peptide applications. Surface Area: 300m2/g Pore Size: 100A Pore Volume: 0.9 pH Range: 2-8 Particle Size: 3um, 5um

HPLC COLUMNS

Pyridine & Phenol Monitor 5m m C18; 150x4.6mmID; 1650psi Zorbax Rx C18; 150x4.6mmID; 1800psi

14

Orochem Technologies Inc.

Inertsil ODS-2: 150x4.6mmID; 2350psi

Symmetry C18; 150x3.9mmID; 2500psi

Kromasil C18; 150x4.6mmID; 1600psi

Mobile Phase: 60% 0.01M KH2PO4 to pH7 with KOH/40% Methanol Flow Rate: 1mL./min. Detection: UV254 Temp.: Ambient All columns packed with 5Mm C18 material as specified. Each horizontal tick mark is equivalent to one minute

15

MonitorTM HPLC Column

Chlorpheniramine Maleate

Mobile Phase: 60:40 50mM KH2PO4, pH3.0:Acetonitrile Column: Monitor 5m m C18, 150x4.6mmID

HPLC COLUMNS

Benefits Improved Peak Shape for Basic and Acidic Compounds Very Reproducible Results Low Back Pressure For Longer Column Life Excellent reproducibility Custom Column Geometries available

Ordering Information
Popular Dimensions:

Phase 3m 100A Silica 3 m 100A C8 3 m 100A C18

50 x 2.0 mm M3BE-003 M3BE-002 M3BE-001

50 x 4.6 mm M3BI-003 M3BI-002 M3BI-001

150 x 4.6 mm M3FI-003 M3FI-002 M3FI-001

Phase 5 m 100A Silica 5 m 100A C8 5 m 100A C18

50 x 2.0 mm M5BE-004 M5BE-006 M5BE-005

50 x 4.6 mm M5BI-004 M5BI-006 M5BI-005

150 x 4.6 mm M5FI-004 M5FI-006 M5FI-005

Microbore/Minibore (in mm) 3 m 100A Silica 3 m 100A C8 3 m 100A C18

50x1.0 M3BD-003 M3BD-002 M3BD-001

100x1.0 M3DD-003 M3DD-003 M3DD-001

150x1.0 M3FD-003 M5FD-002 M3FD-001

16

Orochem Technologies Inc.

Microbore/Minibore (in mm) 5 m 100A Silica 5 m 100A C8 5 m 100A C18

50x1.0 M5BG-004 M5BG-006 M5BG-005

100x1.0 M5DG-004 M5DG-006 M5DG-005

150x1.0 M5FG-004 M5FG-006 M5FG-005

250x1.0 M5HG-004 M5HG-006 M5HG-005

Prep/Se mi-Prep. 5 m 100A S ilica 5 m 100A C8 5 m 100A C18

100x10 mm M5DL-00 4 M5DL-00 6 M5DL-00 5

250x10.0 mm M5HL-004 M5HL-006 M5HL-005

50x20 mm M5BM-004 M5BM-006 M5BM-005

100x21 mm M5DM-004 M5DM-006 M5DM-005

250X2 1 mm M5HM-004 M5HM-006 M5HM-005

250X5 0 mm M5HO-00 4 M5H0-00 6 M5H0-00 5

250x4.6 mm M5HI-004 M5HI-006 M5HI-005

Unique sizes available

Phase 3m 100A Silica 3 m 100A C8 3 m 100A C18

30 x 4.6 mm M3AI-003 M3AI-002 M3AI-001

100 x 4.6 mm M3DI-003 M3DI-002 M3DI-001

150 x 2 mm M3FE-003 M3FE-002 M3FE-001

Phase 3m 100A Silica 3 m 100A C8 3 m 100A C18

30 x 3 mm M3AG-003 M3AG-002 M3AG-001

50 x 3 mm M3BG-003 M3BG-002 M3BG-001

100 x 3 mm M3DG-003 M3DG-002 M3DG-001

125 x 3.0 mm M3EG-003 M3EG-002 M3EG-001

150 x 3 mm M3FG-003 M3FG-002 M3FG-001

250 x 4.6 mm M5HI-004 M5HI-005 M5HI-006

Phase 5 m 100A Silica 5 m 100A C8 5 m 100A C18

30 x 4.6 mm M5AI-004 M5AI-005 M5AI-006

100 x 4.6 mm M5DI-004 M5DI-005 M5DI-006

150 x 2 mm M5FE-004 M5FE-005 M5FE-006

30 x 3 mm M5AG-004 M5AG-005 M5AG-006

50 x 3 mm M5BG-004 M5BG-005 M5BG-006

100 x 3 mm M5DG-004 M5DG-005 M5DG-006

Phase 5 m 100A Silica 5 m 100A C8 5 m 100A C18

125 x 3.0 mm M5EG-004 M5EG-005 M5EG-006

150 x 3 mm M5FG-004 M5FG-005 M5FG-006

250 x 3 mm M5HG-004 M5HG-005 M5HG-006

300 x 4.0 mm M5IS-004 M5IS-005 M5IS-006

250 x 4.6 mm M5HI-004 M5HI-005 M5HI-006

17

PrestigeTM HPLC Column

PrestigeTM HPLC Column

PrestigeTM is Orochem Technologies next-generation of highly base-deactivated sorbents. Double end-capping and narrow particle size range contributes to superior base-deactivation and low backpressure. Surface Area: 320m2/g Pore Size: 100A Pore Volume: 0.9 pH Range: 2-8.5 Particle Size: 3um, 5um, 10um

Ordering Information

HPLC COLUMNS

Popular dimension
Phase 3 m 100A C8 3 m 100A C18 50x2.0 mm P3BE-072 P3BE-073 50x2.0 mm P5BE-071 P5BE-070 50x4.6 mm P3BI-072 P3BI-073 50x4.6 mm P5BI-071 P5BI-070 150x4.6 mm P3FI-072 P3FI-073 150x4.6 mm P5FI-071 P5FI-070 250x4.6 mm P5HI-071 P5HI-070

Phase 5 m 100A C8 5 m 100A C18

Microbore/Minibore (in mm) 3 m 100A C8 3 m 100A C18

50x1.0 P3BD-072 P3BD-073

100x1.0 P3DD-072 P3DD-073

150x1.0 P3FD-072 P3FD-073

100x2.0 P3DE-072 P3DE-073

150x2.0 P3FE-072 P3FE-073

Microbore / Minibore (in mm) 5 m 100A C8 5 m 100A C18

50x1.0 P5BD-071 P5BD-070

100x1.0 P5DD-071 P5DD-070

150x 1.0 P5FD-071 P5FD-070

250x1.0 P5HD-071 P5HD-070

100x 2.0 P5DE-071 P5DE-070

150x2.0 P5DE-071 P5DE-070

250x2.0 P5HE-071 P5HE-070

Standard/Mid Bor e(in mm) 3 m 100A C8 3 m 100A C18

50x3.0 P3BG-072 P3BG-073

100x3.0 P3DG-072 P3DG-073

150x3.0 P3FG-072 P3FG-073

30x4.6 P3AI-072 P3AI-073

100x4.6 P3DI-072 P3DI-073

Standard/Mid Bor e(in mm) 5 m 100A C8 5 m 100A C18

50x3.0 P5BG-071 P5BG-070

100x3.0 P5DG-071 P5DG-070

150x3.0 P5FG-071 P5FG-070

250x3.0 P5HG-071 P5HG-070

30x4.6 P5AI-071 P5AI-070

100x4.6 P5DI-071 P5DI-070

18

Orochem Technologies Inc.

Unique sizes available:

Phase 3 m 100A C8 3 m 100A C18

30 x 4.6 mm P3AI-072 P3AI-073

150 x 2 mm P3FE-072 P3FE-073

30 x 3 mm P3AG-072 P3AG-073

50 x 3 mm P3BG-072 P3BG-073

Phase 3 m 100A C8 3 m 100A C18

100 x 3 mm P3DG-072 P3DG-073

125 x 3.0 mm P3EG-072 P3EG-073

150 x 3 mm P3FG-072 P3FG-073

300 x 3.9 mm P3IS-072 P3IS-073

Phase 5 m 100A C8 5 m 100A C18

30 x 4.6 mm P5AI-071 P5AI-070

150 x 2 mm P5FE-071 P5FE-070

30 x 3 mm P5AG-071 P5AG-070

50 x 3 mm P5BG-071 P5BG-070

Phase 5 m 100A C8 5 m 100A C18

100 x 3 mm P5DG-071 P5DG-070

125 x 3.0 mm P5EG-071 P5EG-070

150 x 3 mm P5FG-071 P5FG-070

250 x 3 mm P5HG-071 P5HG-070

300 x 4.0 mm P5IS-071 P5IS-070

19

ReliachromTM HPLC Column

ReliachromTM
ReliachromTM was designed by Orochem Technologies specifically to yield columns with a high plate count. We use a proprietary type A silica manufacturing process which yields a Gaussian particle distribution, narrow pore-size distribution, and strong resistance to high pH. The result is a material that is perfect for packing high-efficiency columns for general utility. Surface Area: 190m2/g Pore Size: 90A Pore Volume: 0.55 pH Range: 2-8.5 Particle Size: 3um, 5um, 10um

HPLC COLUMNS

Ordering Information
Phase 3 m Silica 3 m 3 m 3 m 3 m 3 m 3 m C1 NH2 SCX SAX Phenyl CN 50 x 2.0 mm RC3BE-930 RC3BE-933 RC3BE-937 RC3BE-940 RC3BE-942 RC3BE-944 RC3BE-946 RC3BE-949 RC3BE-952 50 x 4.6 mm RC3BI-930 RC3BI-933 RC3BI-937 RC3BI-940 RC3BI-942 RC3BI-944 RC3BI-946 RC3BI-949 RC3BI-952 100 x 4.6mm RC3DI-930 RC3DI-933 RC3DI-937 RC3DI-940 RC3DI-942 RC3DI-944 RC3DI-946 RC3DI-949 RC3DI-952 150 x 4.6 mm RC3FI-930 RC3FI-933 RC3FI-937 RC3FI-940 RC3FI-942 RC3FI-944 RC3FI-946 RC3FI-949 RC3FI-952 250 x 4.6 mm RC3HI-930 RC3HI-933 RC3HI-937 RC3HI-940 RC3HI-942 RC3HI-944 RC3HI-946 RC3HI-949 RC3HI-952

3 m C8 3 m C18

Phase 5 m Silica 5 m C1 5 m C4 5 m 5 m 5 m 5 m 5 m 5 m 5 m NH2 SCX SAX Phenyl CN C8 C18

50 x 2.0 mm RC5BE-931 RC5BE-934 RC5BE-935 RC5BE-938 RC5BE-941 RC5BE-943 RC5BE-945 RC5BE-947 RC5BE-950 RC5BE-953

50 x 4.6 mm RC5BI-931 RC5BI-934 RC5BI-935 RC5BI-938 RC5BI-941 RC5BI-943 RC5BI-945 RC5BI-947 RC5BI-950 RC5BI-953

100 x 4.6mm RC5DI-931 RC5DI-934 RC5DI-935 RC5DI-938 RC5DI-941 RC5DI-943 RC5DI-945 RC5DI-947 RC5DI-950 RC5DI-953

150 x 4.6 mm RC5FI-931 RC5FI-934 RC5FI-935 RC5FI-938 RC5FI-941 RC5FI-943 RC5FI-945 RC5FI-947 RC5FI-950 RC5FI-953

250 x 4.6 mm RC5HI-931 RC5HI-934 RC5HI-935 RC5HI-938 RC5HI-941 RC5HI-943 RC5HI-945 RC5HI-947 RC5HI-950 RC5HI-953

20

Orochem Technologies Inc.

Phase 10 m Silica 10 m C4 10 m NH2 10 m CN 10 m C8 10 m C18

100 x 10.0 mm RC9DL-140 RC9DL-141 RC9DL-142 RC9DL-143 RC9DL-145 RC9DL-147

250 x 10.0 RCHL-140 RCHL-141 RCHL-142 RCHL-143 RCHL-145 RCHL-147

100 x 21.2 RC5DM-140 RC5DM-141 RC5DM-142 RC5DM-143 RC5DM-145 RC5DM-147

250 X 21.2 RC9HM-140 RC9HM-141 RC9HM-142 RC9HM-143 RC9HM-145 RC9HM-147

250 X 50 RC9HO-140 RC9HO-141 RC9HO-142 RC9HO-143 RC9HO-145 RC9HO-147

21

ReliasilTM HPLC Column

ReliasilTM HPLC Column

ReliasilTM was designed by Orochem Technologies specifically to yield high plate count. We use a proprietary type A silica manufacturing process which yields a Gaussian particle distribution, narrow pore-size distribution, and strong resistance to high pH. The result is a material that is perfect for packing high-efficiency columns for general utility. Manufactured by Orochem Technologies, Inc., ReliasilTM is an oligomerically derivatized silica gel. It is preferred by many chromatographers for its chemical stability and effectiveness in the reversed phase separations of peptides, pharmaceuticals, and derivatized amino acids.

HPLC COLUMNS
22

Surface Area: 190m2/g Pore Size: 90A Pore Volume: 0.55 pH Range: 2-8.5 Particle Size: 3um, 5um, 10um Benefits 1. Reproducibility 2. Excellent Mechanical/Chemical Stability 3. Superior Efficiency 4. Wide variety of phases 5. Packed to high efficiencies 6. Rugged General-purpose material Available Phases C18 C8 C4 C1 CN (100A and 300A) Hardware Options PEEK Glass-lined Stainless Steel Custom Guard Columns.

Orochem Technologies Inc.

ReliasilTM 5um C18 150 x 4.6 mm

Mobile Phase: CAN/MeOH (7.5:25) Flow Rate: 1.3 ml/min Sample: 1. Vitamin A 2. Vitamin A Acetate 3. Vitamin D2 4. Vitamin D3 5. Vitamin E 6. Vitamin E Acetate 7. Vitamin K1

Ordering Information
Popular Dimensions
Phase 3m Silica 3 m C1 3 m 3 m 3 m 3 m 3 m NH 2 SCX SAX Phenyl CN 50 x 2.0 mm R3BE-100 R3BE-104 R3BE-103 R3BE-107 R3BE-108 R3BE-106 R3BE-105 R3BE-102 R3BE-101 R3BE-118 50 x 4.6 mm R3BI-100 R3BI-104 R3BI-103 R3BI-107 R3BI-108 R3BI-106 R3BI-105 R3BI-102 R3BI-101 R3BI-118 150 x 4.6 mm R3FI-100 R3FI-104 R3FI-103 R3FI-107 R3FI-108 R3FI-106 R3FI-105 R3FI-102 R3FI-101 R3BI-118

3 m C8 3 m C18 3 m ODS-1

23

ReliasilTM HPLC Column

Phase 5 m Silica 5 m C1 5 m C4 5 m NH 2 5 m SCX 5 m SAX 5 m Phenyl

50 x 2.0 mm R5BE-120 R5BE-124 R5BE-123 R5BE-132 R5BE-133 R5BE-134 R5BE-117 R5BE-125 R5BE-122 R5BE-109 R5BE-121 R5BE-119

50 x 4.6 mm R5BI-120 R5BI-124 R5BI-123 R5BI-132 R5BI-133 R5BI-134 R5BI-117 R5BI-125 R5BI-122 R5BI-109 R5BI-121 R5BI-119

150 x 4.6 mm R5FI-120 R5FI-124 R5FI-123 R5FI-132 R5FI-133 R5FI-134 R5FI-117 R5FI-125 R5FI-122 R5FI-109 R5FI-121 R5FI-119

250 x 4.6 mm R5HI-120 R5HI-124 R5HI-123 R5HI-132 R5HI-133 R5HI-134 R5HI-117 R5HI-125 R5HI-122 R5HI-109 R5HI-121 R5HI-119

HPLC COLUMNS

5 m CN 5 m C8 5 m C18 PAH 5 m C18 5 m ODS-1

Phase 10 m Silica 10 m C4 10 m NH2 10 m CN 10 m C8 10 m C18

100 x 10.0 mm R9DL-140 R9DL-141 R9DL-142 R9DL-143 R9DL-145 R9DL-147

250 x 10.0 RHL-140 RHL-141 RHL-142 RHL-143 RHL-145 RHL-147

100 x 21.2 R5DM-140 R5DM-141 R5DM-142 R5DM-143 R5DM-145 R5DM-147

250 X 21.2 R9HM-140 R9HM-141 R9HM-142 R9HM-143 R9HM-145 R9HM-147

250 X 50 R9HO-140 R9HO-141 R9HO-142 R9HO-143 R9HO-145 R9HO-147

Phase 5mm 300A C4 5mm 300A C8 5mm 300A C18

50 x 2.0 R5BE-128 R5BE-127 R5BE-126

50 x 4.6 R5BI-128 R5BI-127 R5BI-126

150 x 4.6 R5FI-128 R5FI-127 R5FI-126

250 x 4.6 R5HI-128 R5HI-127 R5HI-126

24

Orochem Technologies Inc.

Unique sizes available:

P h as e 3 m S ili c a 3 m C1 3 m NH 2 3 m S CX 3 3 3 3 3 3 m m m m m m SA X P he n yl CN C8 C18 O D S -1 3 0 x 4 .6 m m R 3 A I- 10 0 R 3 A I- 10 4 R 3 A I- 10 3 R 3 A I- 10 7 R 3 A I- 10 8 R 3 A I- 10 6 R 3 A I- 10 5 R 3 A I- 10 2 R 3 A I- 10 1 R 3 A I- 11 8 10 0 x 4 .6 m m R 3 D I- 10 0 R 3 D I- 10 4 R 3 D I- 10 3 R 3 D I- 10 7 R 3 D I- 10 8 R 3 D I- 10 6 R 3 D I- 10 5 R 3 D I- 10 2 R 3 D I- 10 1 R 3 D I- 11 8 150 x 2 m m R 3F E - 10 0 R 3F E - 10 4 R 3F E - 10 3 R 3F E - 10 7 R 3F E - 10 8 R 3F E - 10 6 R 3F E - 10 5 R 3F E - 10 2 R 3F E - 10 1 R 3F E - 11 8 30 x 3 mm R 3A G -1 0 0 R 3A G -1 0 4 R 3A G -1 0 3 R 3A G -1 0 7 R 3A G -1 0 8 R 3A G -1 0 6 R 3A G -1 0 5 R 3A G -1 0 2 R 3A G -1 0 1 R 3B G -1 1 8

P h as e 3 m S ili c a 3 m C1 3 m NH 2 3 m S CX 3 m SA X 3 m P he n yl 3 m CN 3 m C8 3 m C18 3 m O D S -1

50 x 3 m m R 3 B G -1 00 R 3 B G -1 04 R 3 B G -1 03 R 3 B G -1 07 R 3 B G -1 08 R 3 B G -1 06 R 3 B G -1 05 R 3 B G -1 02 R 3 B G -1 01 R 3 B G -1 18

1 0 0 x 3 mm R 3 D G -1 00 R 3 D G -1 04 R 3 D G -1 03 R 3 D G -1 07 R 3 D G -1 08 R 3 D G -1 06 R 3 D G -1 05 R 3 D G -1 02 R 3 D G -1 01 R 3 D G -1 18

1 2 5 x 3. 0 m m R 3E G -1 0 0 R 3E G -1 0 4 R 3E G -1 0 3 R 3E G -1 0 7 R 3E G -1 0 8 R 3E G -1 0 6 R 3E G -1 0 5 R 3E G -1 0 2 R 3E G -1 0 1 R 3E G -1 1 8

1 50 x 3 m m R 3 F G -1 00 R 3 F G -1 04 R 3 F G -1 03 R 3 F G -1 07 R 3 F G -1 08 R 3 F G -1 06 R 3 F G -1 05 R 3 F G -1 02 R 3 F G -1 01 R 3 F G -1 18

P h a se 5 m S ilic a 5 m C 1 5 m C 4 5 5 5 5 5 5 5 5 5 m m m m m m m m m N H2 SCX S AX P h e n yl CN C8 C 18 P A H C 18 O D S -1

3 0 x 4. 6 m m R 5 A I- 12 0 R 5 A I- 12 4 R 5 A I- 12 3 R 5 A I- 13 2 R 5 A I- 13 3 R 5 A I- 13 4 R 5 A I- 11 7 R 5 A I- 12 5 R 5 A I- 12 2 R 5 A I- 10 9 R 5 A I- 12 1 R 5 A I- 11 9

1 00 x 4 . 6 m m R 5 D I -1 20 R 5 D I -1 24 R 5 D I -1 23 R 5 D I -1 32 R 5 D I -1 33 R 5 D I -1 34 R 5 D I -1 17 R 5 D I -1 25 R 5 D I -1 22 R 5 D I -1 09 R 5 D I -1 21 R 5 D I -1 19

150 x 2 mm R 5F E - 12 0 R 5F E - 12 4 R R R R R R R R R R 5F E - 12 3 5F E - 13 2 5F E - 13 3 5F E - 13 4 5F E - 11 7 5F E - 12 5 5F E - 12 2 5F E - 10 9 5F E - 12 1 5F E - 11 9

30 x 3 m m R 5A G -1 2 0 R 5A G -1 2 4 R R R R R R R R R R 5A G -1 2 3 5A G -1 3 2 5A G -1 3 3 5A G -1 3 4 5A G -1 1 7 5A G -1 2 5 5A G -1 2 2 5A G -1 0 9 5A G -1 2 1 5A G -1 1 9

P h a se 5 m S ilic a 5 m C 1 5 m C 4 5 5 5 5 5 5 5 5 5 m m m m m m m m m N H2 SCX S AX P h e n yl CN C8 C 18 P A H C 18 O D S -1

50 x 3 mm R 5 B G -1 20 R 5 B G -1 24 R 5 B G -1 23 R 5 B G -1 32 R 5 B G -1 33 R 5 B G -1 34 R 5 B G -1 17 R 5 B G -1 25 R 5 B G -1 22 R 5 B G -1 09 R 5 B G -1 21 R 5 B G -1 19

100 x 3 m m R 5D G - 12 0 R 5D G - 12 4 R 5D G - 12 3 R 5D G - 13 2 R R R R R R R R 5D 5D 5D 5D 5D 5D 5D 5D G - 13 3 G - 13 4 G - 11 7 G - 12 5 G - 12 2 G - 10 9 G - 12 1 G - 11 9

1 2 5 x 3. 0 m m R 5E G -1 2 0 R 5E G -1 2 4 R 5E G -1 2 3 R 5E G -1 3 2 R R R R R R R R 5E G -1 3 3 5E G -1 3 4 5E G -1 1 7 5E G -1 2 5 5E G -1 2 2 5E G -1 0 9 5E G -1 2 1 5E G -1 1 9

1 50 x 3 m m R 5 F G -1 20 R 5 F G -1 24 R 5 F G -1 23 R 5 F G -1 32 R 5 F G -1 33 R 5 F G -1 34 R 5 F G -1 17 R 5 F G -1 25 R 5 F G -1 22 R 5 F G -1 09 R 5 F G -1 21 R 5 F G -1 19

25 0 x 3 m m R 5 H G -1 20 R 5 H G -1 24 R 5 H G -1 23 R 5 H G -1 32 R 5 H G -1 33 R 5 H G -1 34 R 5 H G -1 17 R 5 H G -1 25 R 5 H G -1 22 R 5 H G -1 09 R 5 H G -1 21 R 5 H G -1 19

3 0 0 x 4. 0 m m R 5I S -1 20 R 5I S -1 24 R 5I S -1 23 R 5I S -1 32 R R R R R R R R 5I S -1 33 5I S -1 34 5I S -1 17 5I S -1 25 5I S -1 22 5I S -1 09 5I S -1 21 5I S -1 19

25

OroPureTM HPLC Column

OroPureTM HPLC Column

OroPureTM C18 chemistry is designed on silica where the surface area is relatively low, which makes the retention time shorter. It may be utilized to separate, molecules which have longer retention times. If you routinely use HypersilTM BDS-C18, HypersilTM BDS-C8, ZorbaxTM XDB-C18, and the ZorbaxTM XDB-C8, OroPureTM C18, or OroPureTM C8 respectively will be a good choice to evaluate. Features: - Surface Area: 200m2/g, - Pore Size: 150A, - Particle Size: 5um

HPLC COLUMNS

Ordering Information
OroPureTM
Phase 3m C8 3m C18 50 x 3.0 mm OP3BG-908 OP3BG-906 50 x 4.6 mm OP3BI-908 OP3BI-906 100 x 4.6 mm OP3DI-908 OP3DI-906 150 x 4.6 mm OP3FI-908 OP3FI-906

Phase 5m C8 5m C18

50 x 3.0 mm OP5BG-907 OP5BG-905

50 x 4.6mm OP5BH-907 OP5BH-905

125 x 3.0 mm OP5EH-907 OP5EH-905

250 x 4.6 mm OP5HH-907 OP5HH-905

50 x 4.6mm OP5BI-907 OP5BI-905

150x 4.6 mm OP5FI-907 OP5FI-905

250 x 4.6 mm OP5HI-907 OP5HI-905

OroPureTM DB
Phase 3m C8 3m C18 50 x 2.0 mm OP3BG-912 OP3BG-910 50 x 4.6 mm OP3BI-912 OP3BI-910 150 x 4.6 mm OP3DI-912 OP3DI-910

Phase 5m C8 5m C18

50 x 2.0 mm OP5BG-911 OP5BG-909

50 x 4.6mm OP5BH-911 OP5BH-909

150x 4.6 mm OP5FI-911 OP5FI-909

26

Orochem Technologies Inc.

OroSymTM HPLC Column
OroSymTM C18 column series are bonded to the maximum density to have the highest hydrophobicity and thus the lowest polarity. High C18 moieties result in the lowest interaction with analytes, with silanol, and demonstrate high stability at high pH and the broadest pH range. OroSymTM columns also demonstrate cleaner separation power for isomers, with a near perfect symmetry for basic, acidic and neutral compounds. Features: Surface Area: 45m2/g Pore Size: 130A

Selectivity of Basic, Acidic and Neutral Compounds

1. p-methyl benzoic acid 2. Pyridine 3. Phenol 4. Acetophenone 5. Amino-dimethyl benzene 6. Toluene

150 x 4.6 mm column at 30 oC Mobile Phase: MeOH: AcOH/ NaAc(pH 5.0)= 60 : 40 Flow Rate: 1 mL/min 20uL injection detected at 254 nm

27

OroSymTM HPLC Column

Selectivity of Basic Compounds

HPLC COLUMNS
150 x 4.6 mm column at 30oC Mobile Phase: MeOH: NaH2PO4/ Na2H PO4(pH 7.0)=70 : 30 Flow Rate: 1 mL/min 10uL injection detected at 254 nm

28

Orochem Technologies Inc.

Test of Relative Polarity

Sample: 1. Uracil 2. Pyridine 3. Phenol

Promosil C18 KPyridine/KPhenol = 0.33

MP C18 K Pyridine/KPhenol = 0.45

Luna C18 KPyridine/KPhenol = 0.5

Orosym C18 KPyridine/KPhenol = 0.53

ASB C18 KPyridine/KPhenol = 0.82

150 x 4.6 mm column at 30oC Mobile Phase: MeOH: water = 60:40 Flow Rate: 1ml/min 10uL injection detected at 254nm

29

OroSymTM HPLC Column

Test of Metal Effects

Sample: 1. 2, 7- dihdroxynaphthalene 2. 2, 3- dihydroxynaphthalene

HPLC COLUMNS
150 x 4.6 mm column at 30oC MeOH: water =65 : 35 Flow Rate: 1 mL/min 20uL injection detected at 230 nm

30

Orochem Technologies Inc.

Test of Hydrophobicity
Sample: 1. Urasil 2. Toluene

150 x 4.6 mm column at 25oC MeOH : 25mM KH2PO4 /K2HPO4 (pH 6.0) = 80 : 20 Flow Rate: 1 mL/min u 5uL injection detected at 254 nm u

31

OroSymTM HPLC Column

Test of Lifetime under pH 1.5

Sample: 1. Uracil 2. Phenol 3. Nitrobenzene 4. Naphthalene

HPLC COLUMNS
150 x 4.6 mm column at 50oC MeOH : 0.25% TFA (pH 1.5) = 70 : 30 Flow Rate: 1 mL/min 5uL injection detected at 254 nm

Ordering Information
Phase 3m C8 3m C18 Phase 5m C8 5m C18 100 x 4.6 mm OY3DI-922 OY3DI-920 50 x 3.0 mm OY5BG-921 OY5BG-919 50 x 4.6 mm OY3BI-922 OY3BI-920 50 x 4.6 mm OY5BI-921 OY5BI-919 150 x 2.0 mm OY3FI-922 OY3FI-920 50 x 2.0 mm OY5BH-921 OY5BH-919 50 x 3.0 mm OY3BG-922 OY3BG-920 150 x 4.6 mm OY5FI-921 OY5FI-919 125 x 3.0 mm OY5EH-921 OY5EH-919 250 x 4.6mm OY5HI-921 OY5HI-919

32

Orochem Technologies Inc.

OroSilTM HPLC Column

OroSilTM HPLC columns are uniquely designed for the separation of polar, semi-polar and nonpolar compounds at low to medium pH. The OroSilTM columns are more polar than the OroPureTM columns but are less polar than the OroStarTM HPLC column chemistry. The OroSilTM chemistry provides authentic C18 chemistries, commonly requested and used amongst chromatographers in the industry. With specialized surface treatment, the OroSilTM chemistry makes these columns compatible even with 100% aqueous mobile phase. The OroSilTM columns are known to be stable at pH 1.5-9.0 and are preferentially used against the Waters Symmetry, Inertsil C18 or ODS 3, Zorbax SB, and also the Atlantis dC18 chemistry. Features: Surface Area: 380m2/g, Pore Size: 100A, Particle Size: 5um

Figure 1: Analysis on Inertsil ODS 3 250 x 4.6mm, 5um

Figure 1: Analysis on Inertsil ODS 3 250 x 4.6mm, 5um Figure 1: Analysis on Inertsil ODS 3 250 x 4.6mm, 5um

33

OroSilTM HPLC Column

Ordering Information

Phase 5m C8 5 m C18

50 x 2.0 mm O5BE-923 N/A

50 x3.0 mm O5BG-923 O5BG-903

50 x 4.6 mm O5BI-923 O5BI-903

100 x 10 mm O5DL-923 N/A

100 x 21.2 mm O5DT-923 N/A

125 x 4.0 mm O5EH-923 O5EH-903

Phase

150 x 4.6 mm O5FI-923 O5FI-903

250 x 4.0 mm O5HH-923 O5HH-903

250 x 4.6 mm O5HI-923 O5HI-903

250 x 21.2 mm O5HT-923 N/A

250 x 50 mm O5HO-923 N/A

HPLC COLUMNS
34

5m C8 5 m C18

Orochem Technologies Inc.

OroStarTM HPLC Column
OroStarTM HPLC series of columns are specially designed for the separation of polar compounds at very low to a medium pH value. OroStarTM BS chemistries utilize silica which is end-capped, contains polar groups and in addition have the highest polarity among the OroStarTM columns. These columns are extremely stable at low pH (pH=1) and are alternatives to columns used in the industry. If you use the Waters AtlantisTM, or the ZorbaxTM SB, the OroPureTM C18 or C8 columns may be good alternatives. Features: Surface Area: 200m2/g, Pore Size: 150A Particle Size: 5um

Ordering Information
Phase 3m C8 3m C18 50 x 4.6 mm OT3BI-918 OT3BI-916 100 x 4.6 mm OT3DI-918 OT3DI-916 150 x 4.6 mm OT3FI-918 OT3FI-916 50 x 2.0 mm OT3BG-918 OT3BG-916

Phase 5m C8 5m C18

50 x 2.0 mm OT5BG-917 OT5BG-915

50 x 4.6 mm OR5BH-917 OT5BH-915

125 x 3.0 mm OT5EH-917 OT5EH-915

50 x 4.6 mm OT5BI-917 OT5BI-915

150 x 4.6 mm OT5FI-917 OT5BH-915

35

NuclesilTM HPLC Column

Nucleosil

Ordering Information
Standard Bore
Phase 3mm 100A C8 3mm 100A C18 30x4.6 N3AI-502 N3AI-501 50x4.6 N3BI-502 N3BI-501 100x4.6 N3DI-502 N3DI-501 150x4.6 N3FI-502 N3FI-501 125x4.0 N3EH-502 N3EH-501 250x4.0 N3HH-502 N3HH-501

Microbore/minibore

HPLC COLUMNS

3mm 100A C8 3mm 100A C18

N3BE-502 N3BE-501

N3DE-502 N3DE-501

N3FE-502 N3FE-501

Standard Bore
Phase 5mm 100A NH2 5mm 100A CN 5mm 120A C8 5mm 100A C8 5mm 120A C18 5mm 100A C18 5mm 300A C4 5mm 300A C8 5mm 300A C18 30x4.6 N5AI-526 N5AI-525 N5AI-534 N5AI-523 N5AI-533 N5AI-521 N5AI-538 N5AI-537 N5AI-536 50x4.6 N5BI-526 N5BI-525 N5BI-534 N5BI-523 N5BI-533 N5BI-521 N5BI-538 N5BI-537 N5BI-536 100x4.6 N5DI-526 N5DI-525 N5DI-534 N5DI-523 N5DI-533 N5DI-521 N5DI-538 N5DI-537 N5DI-536 150x4.6 N5FI-526 N5FI-525 N5FI-534 N5FI-523 N5FI-533 N5FI-521 N5FI-538 N5FI-537 N5FI-536 250x4.6 N5HI-526 N5HI-525 N5HI-534 N5HI-523 N5HI-533 N5HI-521 N5HI-538 N5HI-537 N5HI-536 125x4.0 N5EH-526 N5EH-525 N5EH-534 N5EH-523 N5EH-533 N5EH-521 N5EH-538 N5EH-537 N5EH-536 250x4.0 N5HH-526 N5HH-525 N5HH-534 N5HH-523 N5HH-533 N5HH-521 N5HH-538 N5HH-537 N5HH-536

Microbore/minibore
Phase 5mm 100A NH2 5mm 100A CN 5mm 120A C8 5mm 100A C8 5mm 120A C18 5mm 100A C18 5mm 300A C4 5mm 300A C8 5mm 300A C18 50x2.0 N5BE-526 N5BE-525 N5BE-534 N5BE-523 N5BE-533 N5BE-521 N5BE-538 N5BE-537 N5BE-536 100x2 N5DE-526 N5DE-525 N5DE-534 N5DE-523 N5DE-533 N5DE-521 N5DE-538 N5DE-537 N5DE-536 150x2.0 N5FE-526 N5FE-525 N5FE-534 N5FE-523 N5FE-533 N5FE-521 N5FE-538 N5FE-537 N5FE-536 250x2.0 N5HI-526 N5HI-525 N5HI-534 N5HI-523 N5HI-533 N5HI-521 N5HI-538 N5HI-537 N5HI-536

36

Orochem Technologies Inc.

Guard Cartridges

Guard cartridges are protection devices for analytical and preparative HPLC columns. They function as a filter and adsorber of materials that can adversely affect the chemistry of the separation or the lifetime of the column. Their use is highly recommended when injecting anything other than pure filtered standards that elute in a reasonable number (<20) of column volumes. The best way to correctly choose the appropriate guard cartridge is to first consider its objective in the system. There are a multitude of designs and sizes available, and proper choice will provide maximum benefit with minimal effects to the chromatography. Keep in mind that guard cartridges are small columns, and as such they will have backpressure and will contribute to the separation resolution. Because of the effect, even though it is minor, that guard cartridges have on the separation, it is always advised that the stationary phase inside the guard cartridge be the same as that in the analytical column to avoid unexpected results. Typically it is best to use guard cartridges with internal diameters comparable to that of the analytical column. This insures that the linear capacity of the cartridge is adequate for the expected column injection sizes and that the contribution to backpressure is minimal. Frequently chromatographers will choose guard cartridges packed with media of a larger particle size than that in the HPLC column. This also helps to reduce the backpressure contribution from the guard cartridge. Since the length of the guard cartridge packed bed is typically less than 1/10th of the HPLC column, its contribution to the overall efficiency and symmetry is negligible unless the difference in particle sizes between that used in the column and that used in the guard cartridge is greater than an order of magnitude. Since the guard cartridge is like a small HPLC column, proper care must be taken in plumbing it into the system to avoid an increase in extra dead volume. Use minimal tubing lengths from injector to guard cartridge and from guard cartridge to column. The most common question regarding guard cartridges is when they should be changed. If the backpressure increases more than 10% beyond that observed with a fresh guard cartridge, change the cartridge. If a noticeable deterioration of the chromatography occurs after several injections, change the guard cartridge. In summary, guard cartridges are a valuable tool, and with proper use they can help to extend HPLC column life significantly without any adverse effects.

37

Guard Cartridge

Orochem guard columns protect your analytical HPLC column. They remove particulate contaminants and highly adsorptive compounds from samples, prolonging analytical column life. - Guard columns are modular and cartridge replacement is easy. - Guard column cartridge holders are available in stainless steel and PEEK materials. - Both designs are optimized to reduce dead volume and prevent extra column band broadening. - Holders for the cartridges are reusable indefinitely.

Advantages of Using Orochem Guard Column - Higher bed volumes, enhance performance

HPLC COLUMNS

- Virtually no change in chromatography. - Available in analytical, semi-prep and preparative sizes. - Extensive range of chemistries

Analytical 10mm x 2mm ID

Semi-prep 10mm x 10mm ID

10mm x 4.3mm ID

Guard Cartridge Holder

Guard Cartridge Holder

Prep 20mm x 20mm ID

Customized Guard Cartridge Holder

38

Orochem Technologies Inc.

Without Guard Cartridge Ciprofloxacin CiprofloxacinEthylenediamine

With Guard Cartridge Ciprofloxacin CiprofloxacinEthylenediamine Guard Cartridge: Garnet Guard Column
4.3 x 10mm ID Columns: Orosil C18 250mm x 4.6mm

Columns: Orosil C18 250mm x 4.6mm Guard Cartridge: Garnet Guard Column 4.3 x 10 mm ID Acetonitrile: Buffer 13:87 Buffer is 24mM phosphate buffer at pH 3 with triethylamine Flowrate: 1.5ml/min Absorbance: 303nm Acetonitrile: Buffer 13:87 Buffer is 24mM phosphate buffer at pH 3 with Sample: Ciprofloxacin
triethylamine Flowrate: 1.5ml/min Absorbance: 303nm Sample: Ciprofloxacin

39

Guard Cartridge

Matrix
Phase 3 m NH2 3 m C8 3 m AQ 3 m C18 10 x 2 m m R3-138-2 R3-135-2 R3-171-2 R3-136-2 10 x 4 m m R3-138-4 R3-135-4 R3-171-4 R3-136-4 10 x 10 mm R3-138-10 R3-135-10 R3-171-10 R3-136-10 20 x 20 mm R3-138-20 R3-135-20 R3-171-20 R3-136-20

HPLC COLUMNS

Phase 5 m NH2 5 m C8 5 m C 18 5 m C18 AQ 5 m CN

10 x 2 mm R5-137-2 R5-130-2 R5-131-2 R5-170-2 R5-428-2

10 x 4 mm R5-137-4 R5-130-4 R5-131-4 R5-170-4 R5-428-4

10 x 10 mm R5-137-10 R5-130-10 R5-131-10 R5-170-10 R5-428-10

20 x 20 mm R5-137-20 R5-130-20 R5-131-20 R5-170-20 R5-428-20

Phase 10 m NH2 10 m C8 10 m C18 10 m C18 AQ

10 x 2 mm R10-139-2 R10-144-2 R10-146-2 R10-172-2

10 x 4 mm R10-139-4 R10-144-4 R10-146-4 R10-172-4

10 x 10 mm R10-139-10 R10-144-10 R10-146-10 R10-172-10

20 x 20 m m R10-139-20 R10-144-20 R10-148-20 R10-172-10

Monitor
Phase 3 m NH2 3 m C8 3 m C18 3 m CN 3 m Silica 3 m NH2 10 x 2 mm M3 -016-2 M3-002-2 M3-001-2 M3-015-2 M3-003-2 M3-016-2 10 x 4 m m M3-016-4 M3-002-4 M3-001-4 M3-015-4 M3-003-4 M3-016-4 10 x 10 m m M3-016-10 M3-002-10 M3-001-10 M3-015-10 M3-003-10 M3-016-10 20 x 20 mm M3-016-20 M3-002-20 M3-001-20 M3-015-20 M3-003-20 M3-016-20

40

Orochem Technologies Inc.

Phase 5 m NH2 5 m C8 5 m C18 5 m CN 5 m Silica

10 x 2 mm M5-017-2 M5- 006-2 M5 -005-2 M5 -013-2 M5 -004-2

10 x 4 m m M5-017-4 M5-006-4 M5-005-4 M5-013-4 M5-004-4

10 x 10 m m M5-017-10 M5-006-10 M5-005-10 M5-013-10 M5-004-10

20 x 20 mm M5-017-20 M5-006-20 M5-005-20 M5-013-20 M5-004-20

Phase 10 m NH2 10 m C8 10 m C18 10 m CN 10 m Silica

10 x 2 mm M10-018-2 M10-008-2 M10-009-2 M10-020-2 M10-007-2

10 x 4 m m M10-018-4 M10-008-4 M10-009-4 M10-020-4 M10-007-4

10 x 10 mm M10-018-10 M10-008-10 M10-009-10 M10-020-10 M10-007-10

20 x 20 mm M10-018-20 M10-008-20 M10-009-20 M10-020-20 M10-020-20

Prestige

Phase 3 m C8 3 m C18

10 x 2 mm P3-072-2 P3-073-2

10 x 4 m m P3-072-4 P3-073-4

10 x 10 mm P3-072-10 P3-073-10

20 x 20 mm P3-072-20 P3-073-20

Phase 5 m C8 5 m C18

10 x 2 mm P5-071-2 P5-070-2

10 x 4 mm P5-071-4 P5-070-4

10 x 10 mm P5-071-10 P5-070-10

20 x 20 mm P5-071-20 P5-070-20

Phase 10 m C8 10 m C18

10 x 2 mm P10-074-2 P10-075-2

10 x 4 mm P5-074-4 P5-075-4

10 x 10 mm P5-074-10 P5-075-10

20 x 20 mm P5-074-20 P5-075-20

41

Guard Cartridge

Reliasil
Phase 3 m NH2 3 m C8 3 m C18 3 m CN 3 m Silica 3 m SCX 10 x 2 mm R3-103-2 R3-102-2 R3-101-2 R3-105-2 R3-100-2 R3-107-2 10 x 4 m m R3-103-4 R3-102-4 R3-101-4 R3-105-4 R3-100-4 R3-107-4 10 x 10 m m R3-103-10 R3-102-10 R3-101-10 R3-105-10 R3-100-10 R3-107-10 20 x 20 mm R3-103-20 R3-102-20 R3-101-20 R3-105-20 R3-100-20 R3-107-20

HPLC COLUMNS

Phase 5 m NH2 5 m C8 5 m C 18 5 m CN 5 m Silica 5 m SCX

10 x 2 mm R5-132-2 R5-122-2 R5-121-2 R5-125-2 R5-120-2 R5-133-2

10 x 4 m m R5-132-4 R5-122-4 R5-121-4 R5-125-4 R5-120-4 R5-133-4

10 x 10 m m R5-132-10 R5-122-10 R5-121-10 R5-125-10 R5-120-10 R5-133-10

20 x 20 mm R5-132-20 R5-122-20 R5-121-20 R5-125-20 R5-120-20 R5-133-20

Reliachrom
Phase 3 m NH2 3 m C8 3 m C18 3 m CN 3 m Silica 3 m SCX 10 x 2 mm RC3-937-2 RC3-949-2 RC3-952-2 RC3-946-2 RC3-930-2 RC3-940-2 10 x 4 mm RC3-937-4 R3C-949-4 R3C-952-4 R3C-946-4 R3C-930-4 R3C-940-4 10 x 10 mm RC3-937-10 RC3-949-10 RC3-952-10 RC3-946-10 RC3-930-10 RC3-940-10 20 x 20 mm RC3-937-20 RC3-949-20 RC3-952-20 RC3-946-20 RC3-930-20 RC3-940-20

Phase 5 m NH2 5 m C8 5 m C 18 5 m CN 5 m SCX 5 m Silica

10 x 2 mm RC5-938-2 RC5-950-2 RC5-953-2 RC5-947-2 RC5-941-2 RC5-931-2

10 x 4 mm RC5-938-4 RC5-950-4 RC5-953-4 RC5-947-4 RC5-941-4 RC5-931-4

10 x 10 mm RC5-938-10 RC5-950-10 RC5-953-10 RC5-947-10 RC5-941-10 RC5-931-10

20 x 20 mm RC5-938-20 RC5-950-20 RC5-953-20 RC5-947-20 RC5-941-20 RC5-931-20

42

Orochem Technologies Inc.

Phase 10 m NH2 10 m C8 10 m C18 10 m CN 10 m Silica

10 x 2 mm RC10-939-2 RC10-951-2 RC10-954-2 RC10-948-2 RC10-932-2

10 x 4 m m RC10-939-4 RC10-951-4 RC10-954-4 RC10-948-4 RC10-932-4

10 x 10 mm RC10-939-10 RC10-951-10 RC10-954-10 RC10-948-10 RC10-932-10

20 x 20 mm RC10-939-20 RC10-951-20 RC10-954-20 RC10-948-20 RC10-932-20

43

Prep/Semi-prepTM HPLC Column

Prep/semi-prep Columns

Ordering Information
Phase 10 m Silica 10 m C 4 10 m NH2 10 m CN 10 m C 8 10 m C 18 50 x 50 mm R10BO-140 R10BO-141 R10BO-142 R10BO-143 R10BO-145 R10BO-147 100 x 50 mm R10DO-140 R10DO-141 R10DO-142 R10DO-143 R10DO-145 R10DO-147 150 x 50 mm R10FO-140 R10FO-141 R10FO-142 R10FO-143 R10FO-145 R10FO-147 250 x 50 mm R10HO-140 R10HO-141 R10HO-142 R10HO-143 R10HO-145 R10HO-147

HPLC COLUMNS

Phase 10 m Silica 10 m C 4 10 m NH2 10 m CN 10 m C 8 10 m C 18

50x 10 mm R10BL-140 R10BL-141 R10BL-142 R10BL-143 R10BL-145 R10BL-147

100 x 10 mm R10DL-140 R10DL-141 R10DL-142 R10DL-143 R10DL-145 R10DL-147

150 x 10 mm R10FL-140 R10FL-141 R10FL-142 R10FL-143 R10FL-145 R10FL-147

250 x 10 mm R10HL-140 R10HL-141 R10HL-142 R10HL-143 R109HL-145 R10HL-147

Phase 10 m Silica 10 m C 4 10 m NH2 10 m CN 10 m C 8 10 m C 18

50 x 21.2 mm R10BM-140 R10BM-141 R10BM-142 R10BM-143 R10BM-145 R10BM-147

100 x 21.2 mm R10DT-140 R10DT-141 R10DT-142 R10DT-143 R10DT-145 R10DT-147

150 x 20 mm R10FT-140 R10FT-141 R10FT-142 R10FT-143 R10FT-145 R10FT-147

250 x 50 mm R10HO-140 R10HO-141 R10HO-142 R109HO-143 R109HO-145 R10HO-147

Phase 10 m Silica 10 m C 4 10 m NH2 10 m CN 10 m C 8 10 m C 18

50 x 30 mm R10BN-140 R10BN-141 R10BN-142 R10BN-143 R10BN-145 R10BN-147

100 x 30 mm R10DN-140 R10DN-141 R10DN142 R10DN-143 R10DN-145 R10DN-147

150 x 30 mm R10DN-140 R10DN-141 R10DN142 R10DN-143 R10DN-145 R10DN-147

250 x 30 mm R10HN-140 R10HN-141 R10HN142 R10HN-143 R10HN-145 R10HN-147

44

Orochem Technologies Inc.

Test Mix
HPLC column test mixtures are invaluable tools for trouble shooting separation problems. Typically when an HPLC column manufacturer produces a column, the columns efficiency and peak asymmetry is evaluated using a well-characterized test mixture under ideal conditions. Generally these conditions are such that the solutes have reproducible retention with capacity factors in excess of 3, the asymmetry is very close to 1, and the efficiency is at a maximum. The premium HPLC column manufacturers include a sample of text mix with each column sold along with the corresponding test chromatogram. Obviously, testing the column is critical to insure that the column meets specified release criteria. However, the value to the chromatographer can be even greater. When a separation gives unexpected results, it is important to realize that the column may not be the source of the problem. Running the test mixture under prescribed conditions can point to the source of the problem. Some problems are not column-related, such as irreproducible retention times, baseline spikes, and inadequate limits of detection. Problems that may be column-related are: 1. 2. 3. 4. 5. Peak tailing or fronting Overly short or overly long retention times Peak co-elution Poor efficiency High backpressure

Testing the column with the test mix and under release conditions as specified on the release QC chromatogram will reveal whether the problem is due to a poor column or due to poor separation chemistry. Clearly, if the chromatogram obtained from the test mix closely corresponds with the release QC chromatogram, the column is not the source of the problem, and the separation chemistry is the most likely source of the problem. If the chromatogram does not corresponds closely to the release QAC chromatogram, then the column is the source of the problem. Therefore, it is advised that when separation problems arise, simply running the appropriate test mix under prescribed conditions can point to the source of the problem.

45

Test Mix

Test Mix

Reversed Phase Test Mix

Columns C18 C8 CN C6 PHENYL

Solvent

60% Acetonitrile /40% Water

Compone nts Uracil Acetophenone Methyl Benzoate Toluene Naphthalene

Catalog No.

Pr ice Call us!

OCRP5MIX

Test Mix 1. Uracil 2. Acetophenone 3. Methyl Benzoate 4. Naphthalene 5. Toluene Column: ReliaSil C18 5um 250 x 4.6mm Mobile Phase: 60% Acetonitrile / 40% water Flow Rate: 1.0 ml/min Pressure: 1500 psi

HPLC COLUMNS

Test Mix

Columns SAX WAX

Solvent

Components Cytidi ne Adenosine Uridine Guanosine

Catalog No.

Price

Anion Exchange

0.05M KH2PO 4, adjust pH to 3.0 with H 3PO4

OCANEXTM

Call us!

2 1 4

Test Mix 1. Cytidine-5-MonoPhos 2. Adenosine-5-MonoPhos 3. Uridine-5-MonoPhos 4. Guanosine-5-MonoPhos Column: ReliaSil SAX 5um 150 x 4.6mm Mobile Phase: 0.05M KH2PO4/H3PO4 pH3.0 Flow Rate: 1.0 ml/min Pressure: 1000 psi

46

Orochem Technologies Inc.

Test Mix

Columns Silica CN

Solvent

Components Naphthalene Dioctyl Phthalate Diethyl Phthalate Dimethyl Phthalate Cinnamyl Alcohol Naphthalene Dioctyl Phthalate Diethyl Phthalate Dimethyl Phthalate

Catalog No.

Price

Normal Phase-1

3% IPA in Heptane

OCNP1TM

Call us!

Normal Phase-2

Amino

3% IPA in Heptane

OCNP2TM

Call us!

1 2 34

Test Mix 1. Naphthalene 2. Dioctyl Phthalate 3. Diethyl Phthalate 4. Dimethyl Phthalate 5. Cinnamyl Alcohol

Column: ReliaSil Silica 5um 250 x 4.6mm Mobile Phase: 3% Isopropanol in Heptane Flow Rate: 1.0 ml/min Pressure: 700 psi

Test Mix 1. Naphthalene 2. Dioctyl Phthalate 3. Diethyl Phthalate 4. Dimethyl Phthalate

4
Column: ReliaSil Amino 5um 150 x 4.6mm Mobile Phase: 3% Isopropanol in Heptane Flow Rate: 1.0 ml/min Pressure: 400 psi

47

Test Mix

Test Mix

Columns

Solvent

Components Uracil Guanine Cytosine

Catalog No.

Price

Cation Exchange

SCX WCX

0.2M (NH2)H2PO4, adjust pH to 3.5 with H3PO4

OCCTEXTM

Call us!

3
Test Mix 1. Uracil 2. Guanine 3. Cytosine

HPLC COLUMNS

Column: ReliaSil SCX 5um 50 x 4.6mm Mobile Phase: 0,2M (NH4)H2PO4/H3PO4 pH 3.5 Flow Rate: 1.0 ml/min Pressure: 600 psi

General Column Operation Parameters Mobile Phase Solvents/Reagents: Use HPLC grade to avoid problems. Flow Rate/Backpressure: In general, if you keep backpressure under control, flow rate can be whatever you want it to be. Silica-Based HPLC packings used for reversed and normal phase separations can usually withstand pressures higher than what is acceptable by the instrument they are used on. Thus, the pressure guidelines you use should be based on the recommendations given by your HPLC instrument manufacturer. pH Range: General operation parameters for most packings 2.5-7.5 Silica-based materials other than C8 and C18 (ODS) 2.0-8.0 Silica-based monomerically bonded C8 and C18 1.5-9.0 Silica-base polymerically bonded C8 and C18 2.0-13.0 Alumina-based bonded materials Materials adsorbing onto the packing material in your HPLC column typically result in shorter column lifetimes. Guard cartridges/columns increase column lifetime by allowing adsorbtion to occur upstream on the guard cartridge material rather than on the HPLC material. Orochem has wide range of guard columns with extensive range of chemistries.

48

Orochem Technologies Inc.

Orochem Columns Cross Referenced with Industry Columns

Orochem Columns MatrixTM HPLC Columns MatrixTM Industry Columns

Adsorbosphere HS Zorbax Bonus Hyperprep HS Silica PrimeSphere Si PrimeSphere C8 PrimeSphere C8-MC PrimeSphere C8-HC PrimeSphere C4 Basic C4 Cosmosil Vydac201HS C18 Zorbax Rx C18 Betasil C18 BetaBasic C18 Hyperprep HS C18 Betasil C8 Hyperprep HS C8 Aquasil Aqua C18 YMC ODS-AQ ACQUA Atlantis dc 18 Purposher RP 18 (End Cap) Zorbax SB CN Betasil CN Prodigy ODS (2) Prodigy C8 Prodigy ODS(3) Prodigy Phenyl (3)

MatrixTM C18

MatrixTM C8

MatrixTM AQ

MatrixTM CN SB MatrixTM CN MonitorTM HPLC Columns

PrestigeTM HPLC Columns PrestigeTM C18

Hypersil Hypurity ACE C18 Lichrocart Purosphere star RP18e-B-Hi-Q-sil C18 Supersphere RP-18e PhenoSphere-NEXT C18

49

Orochem Columns Cross Referenced with Industry Columns

Orochem Columns PrestigeTM C8 ACE C8

Industry Columns Supersphere, 60 Rp-8 PhenoSphere-NEXT C8

PrestigeTM

PhenoSphere-NEXT Silica PhenoSphere-NEXT CN PhenoSphere-NEXT Phenyl Jsphere Prontosil Vydac, C4

ReliasilTM HPLC Columns ReliasilTM C1

Zorbax TMS Hypersil C1 (SAS) IB-Sil C1 PhenoSphere C1 Ultrasphere Silica Hyperprep Silica IB-Sil Silica Maxsil Silica PhenoSphere Silica SphereClone Silica Spherex Silica Ultremex Silica Novapak Silica Resolve Silica Spherisorb Silica Altima C8 Econosil C8 Econosphere C8 Vydac 208TP C8 ZorBax Rx C8 ZorBax C8 Hyperprep C8 Columbus C8 IB-SIl C8-BD IB-Sil C8 Kingsorb C8 Maxsil C8 PhenoSphere C8 SphereClone C8 Spherex C8 Ultremex C8 Resolve C8 Spherisorb C8 YMC C8 Hypersil C8 (MOS-2)

HPLC COLUMNS
50

ReliasilTM Silica

ReliasilTM C8

ReliasilTM C8 EC

Orochem Technologies Inc.

Orochem Columns ReliasilTM C8 NEC ReliasilTM ODS-1 Industry Columns Hypersil C8 (MOS-1) Hypersil ODS PhenoSphere ODS (1) PhenoSphere ODS (2) SphereClone ODS (1) SphereClone ODS (2) Spherisorb ODS-1 Spherisorb ODS-2 Adsorbosphere C18 Altima C18 Altima Silica Econosil C18 Econosphere C18 RSil C18 RSil C8 Versapak C18 Vydac 218TP C18 Zorbax ODS Hyperprep C18 Columbus C18 IB-Sil C18 IB-Sil C18-BD Kingsorb C18 Maxsil C18 Spherex C18 Spherex C18 IP Ultremex C18 Ultremex C18 IP Jupiter C18 Novapak C18 Resolve C18 HCHROME RP8 Vydac214TP C4 Jupiter C4 ZorBax CN IB-Sil CN PhenoSphere CN Novapak CN Resolve CN Spherisorb CN Hypersil CN (CPS-2) Maxsil CN Hypersil CN (CPS-1)

ReliasilTM C18

ReliasilTM C4

ReliasilTM CN

ReliasilTM CN EC

ReliasilTM CN NEC

51

Orochem Columns Cross Referenced with Industry Columns

Orochem Columns

Industry Columns

ReliasilTM NH2

Hypersil NH2 (APS-2) Maxsil NH2 IB-Sil NH2 SphereClone NH2 Spherex NH2 Spherisorb NH2 Hypersil NH2 (APS-1)

ReliasilTM NH2 NEC

ReliasilTM Phenyl

HPLC COLUMNS

ZorBax Phenyl Hypersil Phenyl Kingsorb Phenyl PhenoSphere Phenyl SphereClone Phenyl Spherex Phenyl Ultremex Phenyl Novapak Phenyl Spherisorb Phenyl YMC Phenyl PhenoSphere SCX Spherisorb SCX PhenoSphere SAX SphereClone SAX Spherex SAX Spherisorb SAX Spherex Diol Hypersil Phenyl-2

ReliasilTM SCX

ReliasilTM SAX

ReliasilTM Diol ReliasilTM Phenyl EC OroPureTM HPLC Columns OroPureTM DB C18

ZorBax XDB C18 Discovery C18 ZorBax XDB C8 Discovery C8

OroPureTM DB C8

OroSilTM HPLC Columns OroSilTM ODS

InertSil ODS2

52

Orochem Technologies Inc.

Orochem Columns Industry Columns InertSil ODS3 OroSymTM HPLC Columns OroSymTM C18

ZorBax Eclipse Plus C18 Luna C18 Symmetry C18 Sunfire C18 Ascentis C18 Luna C8 Ascentis C8

OroSymTM C8

OroStarTM HPLC Columns OroStarTM BS C8

ZorBax SB C8

53

Industry Columns Cross Referenced with Orochem Columns

Industry Columns Cross Referenced with Industry Columns

Industry Columns
Alltech Associates/Vydac/Grace Adsorbosphere C18 Adsorbosphere HS Alltima C8 Alltima C18 Alltima Silica Alphabond C18 Alphabond C8 Alphabond Si Econosil C18 Econosil C8 Econosphere C18 Econosphere C8 Platinum/Platinum EPS Platinum C4/C4 EPS Platinum C8/C8 EPS Platinum C8/C8 EPS Platinum C18/C18 EPS Platinum C18/C18 EPS RSil C18 RSil C8 Versapak C18 Vydac 201TP C18 Vydac 208TP C8 Vydac 218TP C18 Vydac 214TP C4 Vydac 201HS C18

Particle Sizes
S, 5,10 S, 3, 5, 10 S, 5, 10 S, 5, 10 S. 5, 10 I, 5, 10 I, 5, 10 I, 5, 10 I, 5, 10 I, 5, 10 S, 3, 5 S, 3, 5 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 I, 5, 10 I, 5, 10 I, 10 SD, 3, 5, 10 SD, 3, 5, 10 SD, 3, 5, 10 SD, 3, 5, 10 S, 3, 5, 10

Orochem Columns
Reliasil C18 Matrix Reliasil C8 Reliasil C18 Reliasil C18 Simpak C18 Simpak C8 Simpak Reliasil C18 Reliasil C8 Reliasil C18 Reliasil C8 Emerald Emerald C4 Emerald C8 Emerald C8 Emerald C18 Emerald C18 Reliasil C18 Reliasil C18 Reliasil C18 N/A Reliasil C8 Reliasil C18 Reliasil C4 Matrix C18

HPLC COLUMNS

Agilent / Hewlett Packard Zorbax ODS Zorbax C8 Zorbax Rx C8 Zorbax Phenyl Zorbax CN Zorbax TMS Zorbax Rx C18 Zorbax Bonus Zorbax SB C8 Zorbax SB C18 Zorbax SB Phenyl Zorbax SB CN Zorbax XDB C8 Zorbax XDB C18 Zorbax Eclipse Plus C18 Beckman Altex Ultrasphere Silica

S, 5, 7 S, 5, 7 S, 5 S, 3, 5 S, 3, 5, 7 S, 5 S, 5 S, 3.5, 5, 7 S, 3.5, 5, 7 S, 3.5, 5, 7 S, 3.5, 5, 7 S, 3.5, 5, 7 S, 3.5, 5, 7 S, 3.5, 5, 7 S, 3, 5, 10

Reliasil C18 Reliasil C8 Reliasil C8 Reliasil Phenyl Reliasil CN Reliasil C1 Matrix C18 Matrix Orostar BS C8 Orostar BS C18 Matrix Phenyl SB Matrix CN SB Oropure DB C8 Oropure DB C18 Orosym C18

S, 3, 5

Reliasil Silica

Eka Nobel Kromasil Silica

S, 3.5, 5,7,10,13,16

Kromasil Silica

54

Orochem Technologies Inc.

Industry Columns
Kromasil C1 Kromasil C4 Kromasil C8 Kromasil C18 Kromasil NH2 EM Science Lichrosorb Si 60 Lichrosorb Si 100 Lichrosorb RP-18 Lichrosorb RP- 8 Lichrosorb RP-select B Lichrospher Si 60 Lichrospher Si 100 Lichrospher RP-8 Lichrospher RP-8 Endcap Lichrospher RP-18 Lichrospher RP-18 Endcap Lichrospher CN Lichrospher NH2 Lichrospher Diol Lichrospher RP-select B GL Sciences Inertsil Silica Inertsil ODS-2 Inertsil ODS-3 Inertsil ODS-P Inertsil C8 Inertsil C8-3 Inertsil Ph (phenyl) Inertsil Ph-3 Inertsil CN-3 Inertsil C4 Inertsil 80 Inertsil Prep ODS, C8, Si

Particle Sizes
S, 3.5, 5,7,10,13,16 S, 3.5, 5,7,10,13,16 S, 3.5, 5,7,10,13,16 S, 3.5, 5,7,10,13,16 S, 3.5, 5,7,10,13,16

Orochem Columns
Kromasil C1 Kromasil C4 Kromasil C8 Kromasil C18 Kromasil NH2 Lichrosorb Si 60 Lichrosorb Si 100 Lichrosorb RP-18 Lichrosorb RP- 8 Lichrosorb RP-select B Lichrospher Si 60 Lichrospher Si 100 Lichrospher RP-8 Lichrospher RP-8 Endcap Lichrospher RP-18 Lichrospher RP-18 Endcap Lichrospher CN Lichrospher NH2 Lichrospher Diol Lichrospher RP-select B

I, 5, 10 I, 5, 10 I, 5, 10 I, 5, 10 I, 5, 10 S, 3,5,10 S, 5,10 S, 3, 5, 10 S, 3,5,10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10

S, 5 S, 5 S, 3, 5 S, 5 S, 3, 5 S, 5 S, 5 S, 5 S, 5 S, 5 S, 5 S, 10

Orosil ODS Orosil ODS

Hypersil HPLC / Thermo Hypersil ODS Hypersil C8 (MOS-1) Hypersil C8 (MOS-2) Hypersil Phenyl Hypersil Phenyl-2 Hypersil C1 (SAS) Hypersil CN (CPS-1) Hypersil CN (CPS-2) Hypersil NH2 (APS-1) Hypersil NH2 (APS-2) Hypersil BDS C8 Hypersil BDS C18

S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 5 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5 S, 3, 5

Reliasil ODS Reliasil C8 NEC Reliasil C8 EC Reliasil Phenyl Reliasil Phenyl EC Reliasil C1 Reliasil CN NEC Reliasil CN EC Reliasil NH2 NEC Reliasil NH2 EC Emerald C8 Emerald C18

55

Industry Columns Cross Referenced with Orochem Columns

Industry Columns
Hypersil BDS Phenyl Hypersil BDS CN Hypersil Green PAH Hypersil MOS-2 Hypercarb Hyperprep Silica Hyperprep C8 Hyperprep C18 Hyperprep HS Silica Hyperprep HS C8 Hyperprep HS C18 Aquasil Betasil C18 Betasil C8 Betasil CN BetaBasic C18 Deltabond ODS Prism RP (Embedded Polar) Hypersil Hypurity Macherey Nagel Nucleosil Silica Nucleosil AB Nucleosil C18 Nucleosil C8 Nucleosil Phenyl Nucleosil CN Nucleosil NH2 Nucleosil N(CH3)2 Nucleosil SA Nucleosil SB Nucleosil Diol Nucleosil 300 C18 Nucleosil 300 C4 Nucleosil 300 C8 Nucleosil 300 Phenyl Phenomenex Aqua C18 Bondclone Silica Bondclone C18 Columbus C8 Columbus C18 IB-Sil Silica IB-Sil C18 IB-Sil C8 IB-Sil Phenyl IB-Sil C1 IB-Sil CN IB-Sil NH2 IB-Sil C18-BD

Particle Sizes
S, 3, 5 S, 3, 5 S, 4.7 5 S, 7 S, 8, 12, 30 S, 8, 12, 30

Orochem Columns
Emerald Phenyl Emerald CN N/A Reliasil Octyl, 250x4.6, 5um N/A Reliasil Reliasil C8 Reliasil C18 Matrix Matrix C8 Matrix C18 Matrix AQ Matrix C18 Matrix C8 Matrix CN Matrix C18 N/A N/A Prestige C18

HPLC COLUMNS

S, 5 S, 3, 5 S, 3, 5 S, 3, 5 S, 5 S, 5 S, 5 3,5,10

S, (3), 5, 7,10 S, 5 S, (3), 5, 7,10 S, 7 S, 5, 10 S, 5, 10 S, 5, 10 S, 5, 10 S, 7 S, 5, 7, 10 S, 5, 7, 10 S, 5, 7, 10 S,5,7,10

Nucleosil Silica Nucleosil AB Nucleosil C18 Nucleosil C8 Nucleosil Phenyl Nucleosil CN Nucleosil NH2 Nucleosil N(CH3)2 Nucleosil SA Nucleosil SB Nucleosil Diol Nucleosil 300 C18 Nucleosil 300 C4 Nucleosil 300 C8 Nucleosil 300 Phenyl

Spher. 3, 5 Irreg. 10 Irreg. 10 Spher. 5 Spher. 5 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5

Matrix AQ Simpak Simpak Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil

56

Orochem Technologies Inc.

Industry Columns
IB-Sil C8-BD Jupiter C4 Jupiter C5 Jupiter C18 Kingsorb C8 Kingsorb C18 Kingsorb Phenyl Luna Phenyl-Hexyl Luna Silica (2) Luna C5 Luna C8 Luna C8 (2) Luna C18 Luna C18 (2) Luna CN Maxsil Silica Maxsil Alumina Maxsil RP2 Maxsil C3 Maxsil C8 Maxsil C18 Maxsil CN Maxsil NH2 Maxsil NO2 PhenoSphere Si PhenoSphere C1 PhenoSphere C6 PhenoSphere C8 PhenoSphere ODS (1) PhenoSphere ODS (2) PhenoSphere CN PhenoSphere Phenyl PhenoSphere SCX PhenoSphere SAX PhenoSphere CN (2) PhenoSphere-NEXT Silica PhenoSphere-NEXT C8 PhenoSphere-NEXT C18 PhenoSphere-NEXT CN PhenoSphere-NEXT Phenyl Polymerx RP-1 PrimeSphere Si PrimeSphere C8 PrimeSphere C18-MC PrimeSphere C18-HC PrimeSphere C4 PrimeSphere C18-MC Prodigy ODS(2) Prodigy C8 Prodigy ODS (3) Prodigy Phenyl (3)

Particle Sizes
Spher. 3, 5 Spher. 5, 10, 15 Spher. 5, 10, 15 Spher. 5, 10, 15 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5, 10, 15 Spher. 3, 5, 10, 15 Spher. 5, 10 Spher. 5, 10 Spher. 3, 5, 10, 15 Spher. 5, 10 Spher. 3, 5, 10, 15 Spher. 3,5,10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Irreg. 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 5, 10 Spher. 5, 10 Spher. 3, 5, 10 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 3, 5 Spher. 5 Spher. 3, 5, 7, 10 Spher. 5, 10, 15 Spher. 5, 10, 15 Spher. 5, 10 Spher. 5, 10, 15 Spher. 5, 10 Spher. 5, 10 Spher. 5 Spher. 5 Spher. 3, 5, 10 Spher. 5

Orochem Columns
Reliasil Reliasil 300A Reliasil 300A Reliasil 300A Reliasil Reliasil Reliasil N/A No Equivalent at this time No Equivalent at this time Orosym C8 Orosym C8 (2) Orosym C18 Orosym C18(2) No Equivalent at this time Reliasil No Equivalent at this time Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Prestige Prestige Prestige Prestige Prestige N/A Matrix Matrix Matrix Matrix Matrix Matrix Monitor Monitor Monitor Monitor

57

Industry Columns Cross Referenced with Orochem Columns

Industry Columns
Selectosil Silica Selectosil C8 Selectosil C18 Selectosil CN Selectosil SCX Selectosil SAX SphereClone Silica SphereClone C6 SphereClone C8 SphereClone ODS (1) SphereClone ODS (2) SphereClone NH2 SphereClone Phenyl SphereClone SAX Spherex Silica Spherex Phenyl Spherex C8 Spherex C18 Spherex C18 IP Spherex NH2 Spherex Diol Spherex SAX Ultracarb C8 Ultracarb ODS (20) Ultracarb ODS (30) Ultremex Silica Ultremex Phenyl Ultremex C8 Ultremex C18 Ultremex C18 IP

Particle Sizes
Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 5, 10 Spher. 5, 10 Spher. 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 5, 10 Spher. 5, 10 Spher. 5 Spher. 3, 5, 7, 10 Spher. 5, 7, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10 Spher. 3, 5, 10

Orochem Columns
Nucleosil Nucleosil Nucleosil Nucleosil Nucleosil Nucleosil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil Reliasil N/A N/A N/A Reliasil Reliasil Reliasil Reliasil Reliasil

HPLC COLUMNS

Waters Bondapak C18 Bondapak Phenyl Bondapak NH2 Bondapak CN Porasil Silica Novapak C18 Novapak Phenyl Novapak CN Novapak Silica Resolve C18 Resolve C8 Resolve CN Resolve Silica Spherisorb Silica Spherisorb ODS-1 Spherisorb ODS-2 Spherisorb C8

I, 10 I, 10 I, 10 I, 10 I, 10 S, 4 S, 4 S, 4 S, 4 S, 5, 10 S, 5, 10 S, 5, 10 S, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10

Simpak C18 Simpak Phenyl Simpak NH2 Simpak CN Simpak SI Reliasil C18 Reliasil Phenyl Reliasil CN Reliasil Reliasil C18 Reliasil C8 Reliasil CN Reliasil Reliasil Silica Reliasil ODS-1 Reliasil ODS-2 Reliasil C8

58

Orochem Technologies Inc.

Industry Columns
Spherisorb C6 Spherisorb Phenyl Spherisorb CN Spherisorb NH2 Spherisorb SAX Spherisorb SCX Spherisorb PEI Symmetry C18 Symmetry C8 Symmetry Shield Xterra Xbridge Sunfire C18

Particle Sizes
S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 3, 5, 10 S, 5, 10 S, 5, 10 S, 3, 5, 10 S, 3,5,10 S,3, 5, 10 S, 3.5, 5

Orochem Columns
Reliasil C6 Reliasil Phenyl Reliasil CN Reliasil NH2 Reliasil SAX Reliasil SCX Reliasil PEI Orosym C18 Orosym C8 N/A No equivalent No equivalent Orosym C18

S, 3, 5, 10

Whatman Partisil Silica Partisil ODS(3) Partisil ODS(2) Partisil C8 Partisil SAX Partisil SCX YMC ODS-A ODS-A ODS-AM ODS-AQ C8 Phenyl C4 Basic Jsphere ACQUA Supelco Ascentis C18 Ascentis C8 Discovery C18 Discovery C8 ACE C18 HCHROME RP8 ACE C8 ACE PHENYL ACE CN Lichrocart Purosphere star RP18e-BAtlantis dc18 Prontosil Cosmosil

I, 5, 10 I, 5, 10 I, 10 I, 5, 10 I, 10 I, 10

Simpak Simpak C18 Simpak C18 Simpak C8 Reliasil SAX Reliasil SCX

L S, 3,5,7,10,15+ S, 3,5,7,10,15+

S, 4

Reliasil Reliasil Reliasil Matrix AQ Reliasil Reliasil Matrix Matrix Prestige Matrix ODS AQ

S, 3,5,10 S, 3,5,10 S, 3,5,10 S, 3,5,10

Orosym C18 Orosym C8 Oropure DB C18 Oropure DB C8 Prestige C18 Reliasil C18 Prestige C8 Prestige Phenyl Prestige CN Prestige C18 Matrix AQ Prestige Matrix

59

Industry Columns Cross Referenced with Orochem Columns

Industry Columns
Vydac, C4 Purpospher RP18 (End cap Hi-Q-sil C18 Supersphere RP-18e Supersphere, 60 Rp-8

Particle Sizes

Orochem Columns
Prestige Matrix AQ Prestige C18 Prestige C18 Prestige C8

HPLC COLUMNS
60

Orochem Technologies Inc.

Contact Information
Dr. Asha Oroskar President, CEO Tel.: 630.916.0225 E-mail: asha@orochem.com

Dr. Anil Oroskar CTO Tel.: 630.916.0225 E-mail: anil@orochem.com Ved Gulati Operations Director Tel.: 630.916.0225 E-mail: ved@orochem.com Ranjana Gulati Account Manager Tel.: 630.916.0225 E-mail: ranjana@orochem.com

61