ACTIVATION MUSHROOMS CULTURES BY EHF EMI PROMOTES ANTI-INFLAMMATORY & IMMUNITY ACTIVITY OF EXTRACTS

Inesa A. Avagyan 1, Lia A.Minasbekyan1, Arus G. Zhamgaryan 2 ,Siranush G. Nanagulyan1

1. Faculty of Biology, Yerevan State University, str. A. Manoogian, 1, Yerevan, 0025, Armenia, E-mail: inavag@rocketmail.com , 2. Faculty of Pharmacology, Yerevan State Medical University, str. Korune, 2, Yerevan 0025, Armenia

Antiviral drugs based on fungi consist in the ability to block the viral enzyme, synthesis or adsorption of viral nucleic acid, and the introduction of viruses in the mother cells, as well as indirectly - in the immunostimulatory activity of polysaccharides or other complex molecules. The mushroom is also rich by -glycosidase and various peroxidases, necessary for synthesis and cleavage of lignin and polysaccharides and their derivatives. We have shown that at certain frequencies of extremely high frequencies electromagnetic irradiation (EHF EMI) in diapason 45-53 GHZ, some of the enzymes of mushroom rapidly activated in replay to the outer stress: increasing protein content, level of fermentative activity of peroxidase and glucosidase up to 60%. Obtained differences have diversity directed character and depend from frequency and time of exposition by mm-waves [1, 2]. On the basis of obtained data have been suggest to use, treated by mm- waves mycelial culture, for resolving the problem of receiving cheap pharmacological raw material in the abundant value by easy and accessible methods. We have study on determination of anti-inflammatory activity of the above mentioned extracts of mushroom on model of rat ear acute inflammation, induced by xylol. Then the inflammation of an ear induced by a xylol at a rat by whom it has preliminary been intraperitoneal injection of an extracts both from not irradiated and the irradiated mmwaves culture of a mushroom reduces an inflammatory acute by 85 %. Such effective influence of this extracts may be explain by increase of peroxidase activity in culture of a mushroom on 3 day after an irradiation. By HPLC analyze have been revealed increasing of glutamine in 25 time in composition of acid-soluble protein in treated mushroom`s culture, which is probable responsible for such immune replay of organism.
1. Minasbekyan L.A., Nanagyulyan S.G., Avagyan I.A. Increased activity of -glycosidase Pleurotus ostreatus culture in response to the stress.// Immunopathology, allergology, infectology (http://www.immunopatology.by.ru), 2009, 1, p.26-27. 2. Minasbekyan L.A., Avagyan I.A., Nerkararyan A.V., Nanagulyan S.G. Changes in peroxidase activity of Pleurotus ostreatus mushroom culture treated by mm-waves of EMI.// IMMUNOPATOLOGY, ALLERGOLOGY, INFECTOLOGY (http://www.immunopatology.by.ru), 2010, N2, P.47-54.