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5/29/2011

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Topic Outline Blood Collection


Reference: HENRY, Harmening

I. II. III. IV. V. VI. VII.

Donor Registration Donor Selection Medical History Basic Qualifications of the Potential Blood Donor Deferral Phlebotomy Donor Reactions

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Donor Registration
q First step q Consent q Personal Information

Donor Selection
Two Broad Questions: 1. Might the procedure be harmful to donor? 2. Might the donors blood be a risk to the recipient?

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Medical History and Miniphysical


q Medical Questionnaire q Physical Examination

Basic Qualifications of the Potential Blood Donor


q General Appearance q Age: 18-59 years old (60-65, for regular donor) 16-17 years old must have parents consent q Temperature: 37oC

5/29/2011

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Basic Qualifications of the Potential Blood Donor


q Body Weight: 110 lb (50kg) for 450mL collection How to compute for 10.5mL basis collection? > Amount of Blood to be drawn:
Donors weight (lb) x 450 mL 110 lb > Amount of Anticoagulant needed: Allowable amount x 14 100 > Amount of anticoagulant to remove: 63 mL anticoagulant (mL) = Allowable amount (mL)

Basic Qualifications of the Potential Blood Donor


q Pulse: 50 to 100 bpm q Blood pressure : between 90-140mmHg (systolic) between 60-100 mmHg (diastolic) q Hemoglobin: 125g/L (12.5g dL)
Allogeneic Donation q greater or equal to 12.5 g/dL (hemoglobin) q greater or equal to 38% (hematocrit Autologous Donation q H/H: greater than or equal 11 g/dL and 33%

= Anticoagulant needed (mL)

= Anticoagulant to remove (mL)

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Deferral (Permanent)
q q q q q q q q Men who have had sex with another man anytime since 1977 Hemophiliacs Intravenous drug abusers (past or present) Persons who have engaged in sex for money or drugs any time since 1977 Positive for AIDS Symtoms of AIDS History of viral hepatitis after age 11 Donor implicated in post-transfusion hepatitis or AIDS case q q q q q

Deferral (Permanent)
Confirmed positive test for hepatitis B surface antigen (HBsAg) Positive for hepatitis B core antibody Confirmed positive test for hepatitis C antibody Confirmed positive test for Human T-cell lymphotropic virus (HTLV) Malignant solid tumors (except for basal cell carcinoma of the skin and
carcinoma in sit of the cervix.)

q Hematologic malignancies q Chronic cardiopulmonary, liver, or renal disease q Serious abnormal bleeding tendencies

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Deferral (Permanent)
q Those who have ever taken the drug etretinate (Tegison) for the treatment of psoriasis. q Donors who received pituitary-derived human growth hormone q Person who received a tissue transplant of cornea or dura mater

Deferral (Temporary)
q Active disease under treatment such as cold, flu, tuberculosis, syphilis, infections; q curable diseases of the heart, lung, kidney, liver, and gastrointestinal tract; q and treatment with antibiotics

5/29/2011

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Deferral (Three Years)


q An immigrant or refugee coming from an area considered endemic for malaria, three years after departure, q or those who have had diagnosis of malaria, three years after becoming asymptomatic q q q q q q q q

Deferral (One Year)


After hepatitis B immune globulin administration, after therapeutic rabies vaccination (after bite from an animal) Rape victims Healthcare workers Percutaneous exposure to blood or body fluids, Close contact with viral hepatitis Tattoo, Sexual contact with a prostitute or other persons in a high-risk group for AIDS

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Deferral (One Year)


q Incarceration in a jail for more than 72 consecutive hours q Transfusion of blood components q Travel to endemic for malaria (with or without prophylactic therapy)

Deferral ( Two Months)


q Recent blood donation

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Deferral (Six Weeks)


q Following delivery of a baby

Deferral (One Month)


q German measles (rubella) vaccination q After cessation of the drug isotretinoin (Accutane) for acne treatment q After cessation of the drug finasteride (Proscar) for treatment of benign prostatic hyperplasia

5/29/2011

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Deferral (Two Weeks)


q After immune reaction to smallpox vaccination q After vaccination with
q q q oral polio, measles (rubeola), Mumps or yellow fever

Deferral (48 hours)


q Whole blood donation deferred after hemapheresis

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

Donor Reaction
q Mild Reactions
q Syncope or fainting, nausea, or vomiting, hyperventilation, twitching and muscle spasm. Loss of consciousness in addition to mild reactions. Presence of convulsions
q q q Cerebral ischemia Marked hyperventilation Epilepsy

q Moderate Reactions
q q

q Severe Reactions

Immunohematology (Blood Banking) Department of Medical Technol ogy, Institute of Arts and Sciences

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