Code No: R05222301

Set No. 1

II B.Tech Supplimentary Examinations, Aug/Sep 2008 BIO PROCESS ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Mention about dierent component parts of a fermentation process. 2. (a) What is aseptic operation and containment? (b) Describe a typical aseptic, aerobic fermentation process. (c) What is sparger? Describe dierent spargers used in fermentors. [4+12] [4] [4] [2+6]

3. Explain the factors to be considered for developing medium for animal cell culture. [16] 4. (a) What are the important information required for the design of batch sterilisation process. [8] (b) Dene Del factor. Describe the calculation of Del factor during heating and cooling. [2+6=8] 5. Determine the yield coecients (YX/S andYX/02 ) and total amount of oxygen required in a batch reactor of 10000 liters volume with the growth of yeast on glucose as per the equation given C6 H12 O6 +3O2 +0.48NH3 0.48C6 H10 N03 +4.32H2 O + 3.12CO2 Final yeast concentration of 47 gdw/l is required. [16] 6. Discuss about the respiration chain and the electron transport along the respiratory chain. [16] 7. (a) Enumerate the dierence between the cell growth in batch and continuos cultures (b) Explain the kinetics of microbial growth. [8+8]

8. Discuss in details the product kinetics associated with growth with appropriate examples. [16]

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Code No: R05222301

Set No. 2

II B.Tech Supplimentary Examinations, Aug/Sep 2008 BIO PROCESS ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Mention about the Regulatory constraints of bioprocesses. [6+10]

2. What is meant by solid state fermentation? Explain the industrial application of solid state fermentation indicating the microorganisms, substrates and products. [6+10] 3. (a) Explain the use of water as an important constituent for fermentation. (b) Describe the use of buers for media preparation in fermentation. [8+8]

4. Derive the mathematical expression for Nutrient Quality Criterion (Q) in continuous sterilisation and describe the graphical method to optimise temperature-time regime. [16] 5. Determine coecients a, b, c and d (where RQ=0.66) along with the biomass yield coecient and oxygen yield coecient for aerobic degradation of benzoic acid by a mixed culture of microorganisms as represented by the following overall reaction C6 H5 COOH + aO2 +bNH3 cC5 H7 NO2 +dH2 O + eCO2 [16] 6. Enumerate the aerobic catabolism of glucose with emphasis on energetics. [16]

7. (a) Explain the procedure involved in the determination of cell number density and cell mass concentration. (b) Give a short note on simple unstructured kinetic models for microbial growth. [8+8] 8. Describe how the microbial products can be classied along with the equations. [16]

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Code No: R05222301

Set No. 3

II B.Tech Supplimentary Examinations, Aug/Sep 2008 BIO PROCESS ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Discuss in detail dierent unit operations used in manufacture of enzymes. [16]

2. What is meant by solid state fermentation? Explain the industrial application of solid state fermentation indicating the microorganisms, substrates and products. [6+10] 3. Describe in detail the theory of oxygen requirement and supply in industrial fermentation. [16] 4. Describe Arrhenius plot for the calculation of activation energy and derive an expression for heat sterilisation of a pure culture at a constant temperature. [16] 5. Enumerate the dierence in prediction of yield coecients in anaerobic and anaerobic system with appropriate example. [16] 6. Explain the transfer of bioenery via ATP. [16]

7. (a) Explain the procedure involved in the determination of cell number density and cell mass concentration. (b) Give a short note on simple unstructured kinetic models for microbial growth. [8+8] 8. Explain the optimum environmental conditions required for growth and product formation. [16]

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Code No: R05222301

Set No. 4

II B.Tech Supplimentary Examinations, Aug/Sep 2008 BIO PROCESS ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Discuss in detail about dierent commercial enzymes and its applications. [8+8] 2. In a fed batch culture operating with an intermittent addition of glucose solution, values of the following parameters are given at time t =2hours, when the system is at quasi steady state. Culture volume (V) = 1 litre Substrate concentration (S0 ) = 100 gm glucose / litre Limiting rate constant (KS ) = 0.1 gm glucose / litre Initial t amount of biomass in the reactor (X0 ) = 30 gm Nutrient feed ow rate (F) = dv/dt = 200 ml/hour Maximum specic growth rate = 0.3 h1 Yield coecient (YM ) = x/s 0.5 gm cells / gm glucose Determine: (a) the initial culture of the medium (V0 ). (b) Determine the concentration of the growth limiting substrate in the vessel at quasi steady state. (c) Determine the concentration and total amount of biomass in the vessel at t =2 hour (at quasi steady state). (d) If specic rate of product formation (qp ) = 0.2 gm product/cells, P0 = 0 determine the concentration of the product in the vessel at t=2 hour. [4+4+4+4] 3. Explain the factors to be considered for developing medium for animal cell culture. [16] 4. (a) What are the consequences if a foreign microorganism invade a fermentation process. (b) Explain the methods to avoid this contamination. 5. Discuss the following in detail (a) Stoichiometricallly limiting compounds (b) Growth rate limiting medium. [8+8] 6. What are the major steps in aerobic metabolism of hydrocarbons? What are the end products? [16] 7. Explain the dierence between the aerobic and anaerobic growth. [16] [8+8]

8. Give brief notes on structured models for growth and product formation with relevant examples. [16] 1 of 1